Michael Wolgin - Academia.edu (original) (raw)
Papers by Michael Wolgin
European Journal of Dental Education, 2015
Journal of dental education, 2015
The aims of this study were to develop an endodontic simulation model able to implement the elect... more The aims of this study were to develop an endodontic simulation model able to implement the electronic method of working length determination (electronic apex locators, EALs) in a dental school, to evaluate the practicality of this tool for dental students, and to compare the accuracy of working length measurements achieved by the EAL and the radiographic method. A new simulation model was constructed by embedding extracted human teeth in a self-cured resin, along with a conductive medium. After radiographic and electronic working length determinations, root canal instrumentation was performed by students at a dental school in Austria according to the working lengths obtained from the EAL. Subsequently, root apices (n=44) were longitudinally sectioned using a diamond coated bur. Measurements of the distance between the anatomical root apex (ARA) and the apical constriction (AC) as well as between ARA and the ascertained apical point of endodontic instrumentation were performed using...
Case Reports in Dentistry, 2015
This report describes a clinical case with an atypical intraoral sinus tract formation from diagn... more This report describes a clinical case with an atypical intraoral sinus tract formation from diagnosis and treatment to short-term outcome and definitive prosthetic rehabilitation. In detail, the patient underwent conservative nonsurgical root canal treatment followed by guided bone augmentation of the regions involved in periapical inflammation and sinus tract formation. The removal of the inflammatory source of the lesion as well as the affected tissue clearly led to a healing of the surrounding bone tissues. Subsequently, the tooth was reconstructed using a fibreglass post and a metal-ceramic crown; an implant was successfully placed in the previously inflamed bone region.
Objectives: The impact of nicotine on the local innate immune response in the oral cavity is not ... more Objectives: The impact of nicotine on the local innate immune response in the oral cavity is not clearly understood. The aim of the present pilot study was to evaluate the possible effects of nicotine and TNF-α on gene expression of human beta-defensine-1 and -2 in HaCaT keratinocytes. Methods: HaCaTs were cultured in DMEM medium supplemented with 10% FBS, and then split using six-well plates in equal groups. In two groups cells were pretreated either with nicotine (10 μg/ml; well 5) or TNF-α (50 ng/ml; well 6) for 12 h each. The cells in the other wells (1-4) were stimulated for 12 h: negative control (well 1), positive control (TNF-α; 50 ng/ml; well 2), nicotine (10 μg/ml; well 3), TNF-α (50 ng/ml) and nicotine (10 μg/ml; well 4). The pretreated wells 5 and 6 were stimulated with TNF-α (10 μg/ml) and nicotine (50 ng/ml) for 12 h. Total cell RNA was extracted followed by RT-reaction and cDNA synthesis. Samples were analyzed by real-time PCR. Genes of interest were: human beta-defen...
Quintessence international (Berlin, Germany : 1985), 2010
Hyposalivation is represented by a reduced salivary flow rate and can be caused by etiologic fact... more Hyposalivation is represented by a reduced salivary flow rate and can be caused by etiologic factors such as systemic diseases and intake of various medications or by radiotherapy following head and neck cancer. The aim of this review was to compile data about the qualitative and quantitative changes of salivary components during hyposalivation, and to summarize their consequences for oral health. A Medline/PubMed/Scopus search was conducted to identify and summarize articles published in English and German that reported on etiology of hyposalivation and changes in the salivary composition due to hyposalivation of different origins. The search revealed 94 articles, 71 of which were original articles. Apart from the reduction of the salivary flow rate, the quality of saliva is strongly altered because of systemic diseases, medications, and radiotherapy, including increased viscosity and pH shift to more acidic values and changes in salivary protein compositions. Furthermore, hyposali...
Journal of endodontics, 2009
Human beta-defensins (hBDs) are antimicrobial peptides that play an important role in the innate ... more Human beta-defensins (hBDs) are antimicrobial peptides that play an important role in the innate host defense against bacterial invasion, contribute to promotion of adaptive immune responses, and show chemotactic activities. The aim of this study was to compare the gene expression of hBD-1, -2, -3, and -4 in healthy teeth and teeth with pulpitis. Samples of healthy and inflamed dental pulps were obtained from extracted third molars and during treatment of teeth with pulpitis. Gene expression was assessed by using reverse transcriptase reaction and real-time polymerase chain reaction. HBD-2 and hBD -3 were only weakly expressed in healthy and inflamed pulps. In contrast, the expression of hBD-1 and hBD -4 was significantly increased in inflamed compared with healthy pulps. These results suggest that hBD-1 and hBD-4 might play a role in the pulpal host defense.
Clinical oral investigations, Jan 9, 2014
The objectives of this study were to evaluate ex vivo the effects of resin infiltration on the ar... more The objectives of this study were to evaluate ex vivo the effects of resin infiltration on the areal surface roughness of natural non-cavitated proximal subsurface lesions with or without previous deproteinization and to determine differences between E2 and D1 lesions or between premolars and molars. Forty premolars and 40 molars with proximal carious lesions and macroscopically intact surfaces (International Caries Detection and Assessment System (ICDAS) II; code 2) were radiologically assessed and randomly allocated to four groups (with 20 E2 and 20 D1 lesions, respectively). In each group, 10 lesions were deproteinized (NaOCl; 1 %) before etching (HCl; 15 %) and resin infiltration (Icon). Areal surface roughness (Sa) at the most demineralized lesion part (DIAGNOdent) was evaluated topometrically before and after deproteinization, after etching, and after infiltration using focus variation 3D scanning microscopy. Pretreatment with NaOCl (n = 40) had no significant effects on Sa (p...
Journal of Dentistry, 2012
This article appeared in a journal published by Elsevier. The attached copy is furnished to the a... more This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.
Archives of Oral Biology, 2012
Quintessence Publicacion Internacional De Odontologia, 2012
European Journal of Dental Education, 2015
Journal of dental education, 2015
The aims of this study were to develop an endodontic simulation model able to implement the elect... more The aims of this study were to develop an endodontic simulation model able to implement the electronic method of working length determination (electronic apex locators, EALs) in a dental school, to evaluate the practicality of this tool for dental students, and to compare the accuracy of working length measurements achieved by the EAL and the radiographic method. A new simulation model was constructed by embedding extracted human teeth in a self-cured resin, along with a conductive medium. After radiographic and electronic working length determinations, root canal instrumentation was performed by students at a dental school in Austria according to the working lengths obtained from the EAL. Subsequently, root apices (n=44) were longitudinally sectioned using a diamond coated bur. Measurements of the distance between the anatomical root apex (ARA) and the apical constriction (AC) as well as between ARA and the ascertained apical point of endodontic instrumentation were performed using...
Case Reports in Dentistry, 2015
This report describes a clinical case with an atypical intraoral sinus tract formation from diagn... more This report describes a clinical case with an atypical intraoral sinus tract formation from diagnosis and treatment to short-term outcome and definitive prosthetic rehabilitation. In detail, the patient underwent conservative nonsurgical root canal treatment followed by guided bone augmentation of the regions involved in periapical inflammation and sinus tract formation. The removal of the inflammatory source of the lesion as well as the affected tissue clearly led to a healing of the surrounding bone tissues. Subsequently, the tooth was reconstructed using a fibreglass post and a metal-ceramic crown; an implant was successfully placed in the previously inflamed bone region.
Objectives: The impact of nicotine on the local innate immune response in the oral cavity is not ... more Objectives: The impact of nicotine on the local innate immune response in the oral cavity is not clearly understood. The aim of the present pilot study was to evaluate the possible effects of nicotine and TNF-α on gene expression of human beta-defensine-1 and -2 in HaCaT keratinocytes. Methods: HaCaTs were cultured in DMEM medium supplemented with 10% FBS, and then split using six-well plates in equal groups. In two groups cells were pretreated either with nicotine (10 μg/ml; well 5) or TNF-α (50 ng/ml; well 6) for 12 h each. The cells in the other wells (1-4) were stimulated for 12 h: negative control (well 1), positive control (TNF-α; 50 ng/ml; well 2), nicotine (10 μg/ml; well 3), TNF-α (50 ng/ml) and nicotine (10 μg/ml; well 4). The pretreated wells 5 and 6 were stimulated with TNF-α (10 μg/ml) and nicotine (50 ng/ml) for 12 h. Total cell RNA was extracted followed by RT-reaction and cDNA synthesis. Samples were analyzed by real-time PCR. Genes of interest were: human beta-defen...
Quintessence international (Berlin, Germany : 1985), 2010
Hyposalivation is represented by a reduced salivary flow rate and can be caused by etiologic fact... more Hyposalivation is represented by a reduced salivary flow rate and can be caused by etiologic factors such as systemic diseases and intake of various medications or by radiotherapy following head and neck cancer. The aim of this review was to compile data about the qualitative and quantitative changes of salivary components during hyposalivation, and to summarize their consequences for oral health. A Medline/PubMed/Scopus search was conducted to identify and summarize articles published in English and German that reported on etiology of hyposalivation and changes in the salivary composition due to hyposalivation of different origins. The search revealed 94 articles, 71 of which were original articles. Apart from the reduction of the salivary flow rate, the quality of saliva is strongly altered because of systemic diseases, medications, and radiotherapy, including increased viscosity and pH shift to more acidic values and changes in salivary protein compositions. Furthermore, hyposali...
Journal of endodontics, 2009
Human beta-defensins (hBDs) are antimicrobial peptides that play an important role in the innate ... more Human beta-defensins (hBDs) are antimicrobial peptides that play an important role in the innate host defense against bacterial invasion, contribute to promotion of adaptive immune responses, and show chemotactic activities. The aim of this study was to compare the gene expression of hBD-1, -2, -3, and -4 in healthy teeth and teeth with pulpitis. Samples of healthy and inflamed dental pulps were obtained from extracted third molars and during treatment of teeth with pulpitis. Gene expression was assessed by using reverse transcriptase reaction and real-time polymerase chain reaction. HBD-2 and hBD -3 were only weakly expressed in healthy and inflamed pulps. In contrast, the expression of hBD-1 and hBD -4 was significantly increased in inflamed compared with healthy pulps. These results suggest that hBD-1 and hBD-4 might play a role in the pulpal host defense.
Clinical oral investigations, Jan 9, 2014
The objectives of this study were to evaluate ex vivo the effects of resin infiltration on the ar... more The objectives of this study were to evaluate ex vivo the effects of resin infiltration on the areal surface roughness of natural non-cavitated proximal subsurface lesions with or without previous deproteinization and to determine differences between E2 and D1 lesions or between premolars and molars. Forty premolars and 40 molars with proximal carious lesions and macroscopically intact surfaces (International Caries Detection and Assessment System (ICDAS) II; code 2) were radiologically assessed and randomly allocated to four groups (with 20 E2 and 20 D1 lesions, respectively). In each group, 10 lesions were deproteinized (NaOCl; 1 %) before etching (HCl; 15 %) and resin infiltration (Icon). Areal surface roughness (Sa) at the most demineralized lesion part (DIAGNOdent) was evaluated topometrically before and after deproteinization, after etching, and after infiltration using focus variation 3D scanning microscopy. Pretreatment with NaOCl (n = 40) had no significant effects on Sa (p...
Journal of Dentistry, 2012
This article appeared in a journal published by Elsevier. The attached copy is furnished to the a... more This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.
Archives of Oral Biology, 2012
Quintessence Publicacion Internacional De Odontologia, 2012