Midori Fukaya - Academia.edu (original) (raw)
Papers by Midori Fukaya
Rosalia batesi Harold (Cerambycidae) is a hardwood boring species endemic to Japan. We investigat... more Rosalia batesi Harold (Cerambycidae) is a hardwood boring species endemic to Japan. We investigated the adult mating behavior of this species in the field and laboratory. Most males appeared on mating sites before noon, significantly earlier than females did, in field observations. The female approached and contacted the male; the male responded and started the successive mating sequence, comprising mounting, copulation, and appeasement behavior. Before the encounter, the male raised its fore and mid legs and bent the abdominal tip ventrally. Next, a peculiarly structured bifurcate tip was exposed with opening and closing motion, which can be observed in the entire family Cerambycidae and is thought to be associated with the emission of volatile male sex pheromones. Male and female orientation toward conspecifics was examined using T-shaped olfactometers in four combinations (male–male, female–male, female–female, male–female). Males exclusively attracted females, indicating the exi...
Longhorn beetles (Coleoptera: Cerambycidae) utilize pheromones to find and recognize their mates.... more Longhorn beetles (Coleoptera: Cerambycidae) utilize pheromones to find and recognize their mates. Long-range attractant pheromones have recently been chemically identified from a number of cerambycid species. In contrast, knowledge on the contact pheromones in cerambycids, which are essential for the recognition of mates, is limited. In this chapter, we first overview the volatile pheromones in Cerambycidae. We then describe the identification of contact pheromones in two cerambycid species, the yellow-spotted longicorn beetle Psacothea hilaris and the white-spotted longicorn beetle Anoplophora malasiaca. The contact pheromone of the former species consists of a single hydrocarbon, whereas that of the latter species is a markedly complex mixture of 15 components (8 hydrocarbons, 4 aliphatic ketones, and 3 lactones), the identification of which was achieved through research spanning a quarter of century. Lastly, difficulties and problems in the studies of contact pheromones in ceramb...
Juntendo Medical Journal, 2021
Oncology Letters
The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to di... more The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to diagnose colorectal cancer using blood samples. The system uses the 'polymeric CTC-chip,' (CTC-chip), which is a microfluidic device that is used for CTC isolation. CTCs are considered sensitive diagnostic biomarkers. However, their concentration in the peripheral blood is low and requires highly sensitive and specific capturing techniques. The capture efficiency of the polymeric CTC-chip was first assessed using cell suspensions of the colorectal cancer cell line HCT-116, which was reported as 90.9% in a phosphate-buffered saline suspension and 65.0% in the blood. The CTC-chip was then used to detect CTCs in blood samples obtained from 13 patients with stage II-IV colorectal cancer. On average, the CTCs/ml was lower in patients with stages II and III colorectal cancer (3.3±2.3) than in those with stage IV (7.0±6.2). In patients with stages II-IV, 92% had ≥1 CTC per ml, which was significantly higher than the positive rate (15%) detected using the carbohydrate antigen 19-9 test (CA19-9). Furthermore, CTCs were detected in all patients with stage II and III colorectal cancer, including a number of patients with negative results for the carcinoembryonic antigen (CEA) and CA19-9 tests. With the polymeric CTC-chip detection system, CTCs can be effective cancer markers, particularly for patients with stage II and III colorectal cancer who often exhibit negative conventional serum marker test results. The CTC-chip system may also facilitate the detection of cancer progression based on CTC concentration.
Journal of Chemical Ecology
The elytra of females of the white-spotted longhorn beetle, Anoplophora malasiaca (Coleoptera: Ce... more The elytra of females of the white-spotted longhorn beetle, Anoplophora malasiaca (Coleoptera: Cerambycidae), are coated with a contact sex pheromone, which was previously shown to be composed of at least three chemical groups. Individually, the chemical groups had little pheromonal activity, but a blend of all three exhibited activity equal to that of the crude female extract. Two groups are female-specific aliphatic hydrocarbons and ketones, which were previously synthesized and confirmed to elicit mating behavior. The third group consists of three lactones, gomadalactones A, B, and C, whose chemical structures were previously identified. These have now been synthesized, and the contact sex pheromone activities of synthetic gomadalactones A, B, and C, and the diastereomer of gomadalactone C, were tested in bioassays in this study. When tested in combination with synthetic hydrocarbons and ketones at the same doses as found in female elytra extract, the individual gomadalactones and a blend showed potent pheromonal activity equivalent to that of the crude extract of the elytra of female beetles. This completes the identification of the essential components of the contact sex pheromone of A. malasiaca. Redundancy of components in the hydrocarbon and ketone groups required to elicit mating behavior was observed previously, and this was also true for the gomadalactones.
Applied Entomology and Zoology
The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerg... more The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerging invader in Japan and has become a major pest of cherry blossom trees, peaches, prunes and Japanese apricots. The male-produced aggregation-sex pheromone, recently identified as (E)-2-cis-6,7-epoxynonenal, has two enantiomers. Here, we determined which enantiomer the male produces and tested the responses of beetle antennae to the enantiomers using electroantennography. The male produced only the (6R,7S)-enantiomer, but both enantiomers elicited responses from the antennae of female beetles. Antennae of males also responded to both enantiomers. In field trials, the (6R,7S)-enantiomer and the racemic mixture were equally attractive to both sexes of the beetle, whereas the unnatural (6S,7R)-enantiomer did not attract any beetles.
Zoological Letters
Background: Vibrational senses are vital for plant-dwelling animals because vibrations transmitte... more Background: Vibrational senses are vital for plant-dwelling animals because vibrations transmitted through plants allow them to detect approaching predators or conspecifics. Little is known, however, about how coleopteran insects detect vibrations. Results: We investigated vibrational responses of the Japanese pine sawyer beetle, Monochamus alternatus, and its putative sense organs. This beetle showed startle responses, stridulation, freezing, and walking in response to vibrations below 1 kHz, indicating that they are able to detect low-frequency vibrations. For the first time in a coleopteran species, we have identified the sense organ involved in the freezing behavior. The femoral chordotonal organ (FCO), located in the mid-femur, contained 60-70 sensory neurons and was distally attached to the proximal tibia via a cuticular apodeme. Beetles with operated FCOs did not freeze in response to low-frequency vibrations during walking, whereas intact beetles did. These results indicate that the FCO is responsible for detecting lowfrequency vibrations and mediating the behavioral responses. We discuss the behavioral significance of vibrational responses and physiological functions of FCOs in M. alternatus. Conclusions: Our findings revealed that substrate vibrations mediate behavioral responses via femoral chordotonal organs in M. alternatus.
Scientific reports, Jan 4, 2017
The longhorned beetle Aromia bungii (Coleoptera: Cerambycidae) is a major pest of stone fruit tre... more The longhorned beetle Aromia bungii (Coleoptera: Cerambycidae) is a major pest of stone fruit trees in the genus Prunus, including cherries, apricots, and peaches. Its native range includes China, Korea, Mongolia, and eastern Russia, but it has recently invaded and become established in several countries in Europe, and Japan, and it has been intercepted in shipments coming into North America and Australia. Here, we report the identification of its male-produced aggregation pheromone as the novel compound (E)-2-cis-6,7-epoxynonenal. In field trials in its native range in China, and in recently invaded areas of Japan, the pheromone attracted both sexes of the beetle. Thus, the pheromone should find immediate use in worldwide quarantine surveillance efforts to detect the beetle in incoming shipments. The pheromone will also be a crucial tool in ongoing efforts to eradicate the beetle from regions of the world that it has already invaded.
Entomological Science, Jun 25, 2000
Photochemistry and photobiology
Plant-dwelling mites are potentially exposed to solar ultraviolet-B (UVB) radiation that causes d... more Plant-dwelling mites are potentially exposed to solar ultraviolet-B (UVB) radiation that causes deleterious and often lethal effects, leading most mites to inhabit the lower (underside) leaf surfaces. However, in species of spider mite belonging to the Genus Panonychus, a substantial portion of individuals occur on upper leaf surfaces. We investigated whether the upper leaf surfaces of citrus trees are favorable for P. citri, and to what extent they are tolerant to UVB radiation. If eggs are not adequately protected from UVB damage, females may avoid ovipositing on the upper surfaces of sunny leaves. To test this, we conducted laboratory experiments using a UVB lamp, and semioutdoor manipulative experiments. As a result, P. citri eggs are tolerant to UVB. Field studies revealed that the ratio of eggs and adult females on upper leaf surfaces were larger for shaded than for sunny leaves. However, 64-89% of eggs hatched successfully even on sunny upper leaf surfaces. Nutritional evalua...
![Research paper thumbnail of Gomadalactones A, B, and C: novel 3-oxabicyclo[3.3.0]octane compounds in the contact sex pheromone of the white-spotted longicorn beetle, Anoplophora malasiaca](https://attachments.academia-assets.com/80079605/thumbnails/1.jpg)
](Tetrahedron Letters, 2007
Physiological Entomology, 2008
Physiological Entomology, 2010
ABSTRACT Adults of the white grub beetle Dasylepida ishigakiensis Niijima et Kinoshita (Coleopter... more ABSTRACT Adults of the white grub beetle Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scarabaeidae) emerge from the soil around dusk for mating on subtropical islands. The present study examines the factors controlling the emergence of males in the laboratory. There are two steps involved. Standby behaviour (i.e. insect head appears at the soil surface) can be observed for several hours before the beetles actually emerge for mating. The standby behaviour is facilitated by warm conditions, although the proportion of standby individuals is influenced not only by the temperature on that day, but also by that on the previous day. Experiments in which beetles are exposed to photoperiod and thermoperiod combinations, in and out of phase, show that temperature is more important in inducing standby and emerging behaviour than light alone. For the second step, factors such as temperature, light and the presence of the female sex pheromone determine whether males will leave the standby position and emerge onto the ground. The female sex pheromone stimulates standby beetles to exhibit emerging and wing vibration behaviours, although the effect depends on when it is presented to beetles. Beetles burrow back into the soil; this behaviour is influenced by illumination and time of the day but not by temperature. The results suggest that D. ishigakiensis possesses a sophisticated mechanism controlling male emergence from the soil.
Journal of Insect Conservation, 2008
... Midori-no-Tokuhon 43:811816 (in Japanese) Someya T (1998) The present state of the damselfly... more ... Midori-no-Tokuhon 43:811816 (in Japanese) Someya T (1998) The present state of the damselfly, Mortonagrion hirosei. Nature Insects 33(10):48 (in Japanese) Southwood TRE, Henderson PA (2000) Ecological methods, 3rd edn. ...
Journal of Ethology, 2012
ABSTRACT In order to understand the visual orientation mechanisms of the male black chafer Holotr... more ABSTRACT In order to understand the visual orientation mechanisms of the male black chafer Holotrichia loochooana loochooana to females, we examined male behavioral response to colored objects (lures) when female sex pheromone was present. In the field, males landed more frequently on darker lures than on lighter ones of the same color. Among lures of different colors but with the same brightness, males preferred landing on black, blue, or red ones to green or grey ones. The reflectance intensities of preferred lures were weaker than those of green and grey in the range of 540–580 nm. This range is considered to affect choice by males. When females perch on the leaf edges of the food plant to adopt a calling posture, the dominant background color is that of the leaves (i.e., green). This means that the landing target forms a silhouette (dark spot) which is prominent against a background of green leaves, providing maximum visual acuity for mating.
Journal of Chemical Ecology, 1996
major component of female contact sex pheromone of the yellow-spotted longicorn beetle, Psacothea... more major component of female contact sex pheromone of the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe), was isolated from the elytra and identified as (Z)-21-methyt-8-pentatriacontene. The synthetic compound released the typical mating behavior incIuding holding, mounting, and abdominal bending in males, although its activity was considerably lower than the extract of female elytra when treated on a gelatin capsule as an artificial female model.
Rosalia batesi Harold (Cerambycidae) is a hardwood boring species endemic to Japan. We investigat... more Rosalia batesi Harold (Cerambycidae) is a hardwood boring species endemic to Japan. We investigated the adult mating behavior of this species in the field and laboratory. Most males appeared on mating sites before noon, significantly earlier than females did, in field observations. The female approached and contacted the male; the male responded and started the successive mating sequence, comprising mounting, copulation, and appeasement behavior. Before the encounter, the male raised its fore and mid legs and bent the abdominal tip ventrally. Next, a peculiarly structured bifurcate tip was exposed with opening and closing motion, which can be observed in the entire family Cerambycidae and is thought to be associated with the emission of volatile male sex pheromones. Male and female orientation toward conspecifics was examined using T-shaped olfactometers in four combinations (male–male, female–male, female–female, male–female). Males exclusively attracted females, indicating the exi...
Longhorn beetles (Coleoptera: Cerambycidae) utilize pheromones to find and recognize their mates.... more Longhorn beetles (Coleoptera: Cerambycidae) utilize pheromones to find and recognize their mates. Long-range attractant pheromones have recently been chemically identified from a number of cerambycid species. In contrast, knowledge on the contact pheromones in cerambycids, which are essential for the recognition of mates, is limited. In this chapter, we first overview the volatile pheromones in Cerambycidae. We then describe the identification of contact pheromones in two cerambycid species, the yellow-spotted longicorn beetle Psacothea hilaris and the white-spotted longicorn beetle Anoplophora malasiaca. The contact pheromone of the former species consists of a single hydrocarbon, whereas that of the latter species is a markedly complex mixture of 15 components (8 hydrocarbons, 4 aliphatic ketones, and 3 lactones), the identification of which was achieved through research spanning a quarter of century. Lastly, difficulties and problems in the studies of contact pheromones in ceramb...
Juntendo Medical Journal, 2021
Oncology Letters
The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to di... more The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to diagnose colorectal cancer using blood samples. The system uses the 'polymeric CTC-chip,' (CTC-chip), which is a microfluidic device that is used for CTC isolation. CTCs are considered sensitive diagnostic biomarkers. However, their concentration in the peripheral blood is low and requires highly sensitive and specific capturing techniques. The capture efficiency of the polymeric CTC-chip was first assessed using cell suspensions of the colorectal cancer cell line HCT-116, which was reported as 90.9% in a phosphate-buffered saline suspension and 65.0% in the blood. The CTC-chip was then used to detect CTCs in blood samples obtained from 13 patients with stage II-IV colorectal cancer. On average, the CTCs/ml was lower in patients with stages II and III colorectal cancer (3.3±2.3) than in those with stage IV (7.0±6.2). In patients with stages II-IV, 92% had ≥1 CTC per ml, which was significantly higher than the positive rate (15%) detected using the carbohydrate antigen 19-9 test (CA19-9). Furthermore, CTCs were detected in all patients with stage II and III colorectal cancer, including a number of patients with negative results for the carcinoembryonic antigen (CEA) and CA19-9 tests. With the polymeric CTC-chip detection system, CTCs can be effective cancer markers, particularly for patients with stage II and III colorectal cancer who often exhibit negative conventional serum marker test results. The CTC-chip system may also facilitate the detection of cancer progression based on CTC concentration.
Journal of Chemical Ecology
The elytra of females of the white-spotted longhorn beetle, Anoplophora malasiaca (Coleoptera: Ce... more The elytra of females of the white-spotted longhorn beetle, Anoplophora malasiaca (Coleoptera: Cerambycidae), are coated with a contact sex pheromone, which was previously shown to be composed of at least three chemical groups. Individually, the chemical groups had little pheromonal activity, but a blend of all three exhibited activity equal to that of the crude female extract. Two groups are female-specific aliphatic hydrocarbons and ketones, which were previously synthesized and confirmed to elicit mating behavior. The third group consists of three lactones, gomadalactones A, B, and C, whose chemical structures were previously identified. These have now been synthesized, and the contact sex pheromone activities of synthetic gomadalactones A, B, and C, and the diastereomer of gomadalactone C, were tested in bioassays in this study. When tested in combination with synthetic hydrocarbons and ketones at the same doses as found in female elytra extract, the individual gomadalactones and a blend showed potent pheromonal activity equivalent to that of the crude extract of the elytra of female beetles. This completes the identification of the essential components of the contact sex pheromone of A. malasiaca. Redundancy of components in the hydrocarbon and ketone groups required to elicit mating behavior was observed previously, and this was also true for the gomadalactones.
Applied Entomology and Zoology
The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerg... more The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerging invader in Japan and has become a major pest of cherry blossom trees, peaches, prunes and Japanese apricots. The male-produced aggregation-sex pheromone, recently identified as (E)-2-cis-6,7-epoxynonenal, has two enantiomers. Here, we determined which enantiomer the male produces and tested the responses of beetle antennae to the enantiomers using electroantennography. The male produced only the (6R,7S)-enantiomer, but both enantiomers elicited responses from the antennae of female beetles. Antennae of males also responded to both enantiomers. In field trials, the (6R,7S)-enantiomer and the racemic mixture were equally attractive to both sexes of the beetle, whereas the unnatural (6S,7R)-enantiomer did not attract any beetles.
Zoological Letters
Background: Vibrational senses are vital for plant-dwelling animals because vibrations transmitte... more Background: Vibrational senses are vital for plant-dwelling animals because vibrations transmitted through plants allow them to detect approaching predators or conspecifics. Little is known, however, about how coleopteran insects detect vibrations. Results: We investigated vibrational responses of the Japanese pine sawyer beetle, Monochamus alternatus, and its putative sense organs. This beetle showed startle responses, stridulation, freezing, and walking in response to vibrations below 1 kHz, indicating that they are able to detect low-frequency vibrations. For the first time in a coleopteran species, we have identified the sense organ involved in the freezing behavior. The femoral chordotonal organ (FCO), located in the mid-femur, contained 60-70 sensory neurons and was distally attached to the proximal tibia via a cuticular apodeme. Beetles with operated FCOs did not freeze in response to low-frequency vibrations during walking, whereas intact beetles did. These results indicate that the FCO is responsible for detecting lowfrequency vibrations and mediating the behavioral responses. We discuss the behavioral significance of vibrational responses and physiological functions of FCOs in M. alternatus. Conclusions: Our findings revealed that substrate vibrations mediate behavioral responses via femoral chordotonal organs in M. alternatus.
Scientific reports, Jan 4, 2017
The longhorned beetle Aromia bungii (Coleoptera: Cerambycidae) is a major pest of stone fruit tre... more The longhorned beetle Aromia bungii (Coleoptera: Cerambycidae) is a major pest of stone fruit trees in the genus Prunus, including cherries, apricots, and peaches. Its native range includes China, Korea, Mongolia, and eastern Russia, but it has recently invaded and become established in several countries in Europe, and Japan, and it has been intercepted in shipments coming into North America and Australia. Here, we report the identification of its male-produced aggregation pheromone as the novel compound (E)-2-cis-6,7-epoxynonenal. In field trials in its native range in China, and in recently invaded areas of Japan, the pheromone attracted both sexes of the beetle. Thus, the pheromone should find immediate use in worldwide quarantine surveillance efforts to detect the beetle in incoming shipments. The pheromone will also be a crucial tool in ongoing efforts to eradicate the beetle from regions of the world that it has already invaded.
Entomological Science, Jun 25, 2000
Photochemistry and photobiology
Plant-dwelling mites are potentially exposed to solar ultraviolet-B (UVB) radiation that causes d... more Plant-dwelling mites are potentially exposed to solar ultraviolet-B (UVB) radiation that causes deleterious and often lethal effects, leading most mites to inhabit the lower (underside) leaf surfaces. However, in species of spider mite belonging to the Genus Panonychus, a substantial portion of individuals occur on upper leaf surfaces. We investigated whether the upper leaf surfaces of citrus trees are favorable for P. citri, and to what extent they are tolerant to UVB radiation. If eggs are not adequately protected from UVB damage, females may avoid ovipositing on the upper surfaces of sunny leaves. To test this, we conducted laboratory experiments using a UVB lamp, and semioutdoor manipulative experiments. As a result, P. citri eggs are tolerant to UVB. Field studies revealed that the ratio of eggs and adult females on upper leaf surfaces were larger for shaded than for sunny leaves. However, 64-89% of eggs hatched successfully even on sunny upper leaf surfaces. Nutritional evalua...
Tetrahedron Letters, 2007
Physiological Entomology, 2008
Physiological Entomology, 2010
ABSTRACT Adults of the white grub beetle Dasylepida ishigakiensis Niijima et Kinoshita (Coleopter... more ABSTRACT Adults of the white grub beetle Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scarabaeidae) emerge from the soil around dusk for mating on subtropical islands. The present study examines the factors controlling the emergence of males in the laboratory. There are two steps involved. Standby behaviour (i.e. insect head appears at the soil surface) can be observed for several hours before the beetles actually emerge for mating. The standby behaviour is facilitated by warm conditions, although the proportion of standby individuals is influenced not only by the temperature on that day, but also by that on the previous day. Experiments in which beetles are exposed to photoperiod and thermoperiod combinations, in and out of phase, show that temperature is more important in inducing standby and emerging behaviour than light alone. For the second step, factors such as temperature, light and the presence of the female sex pheromone determine whether males will leave the standby position and emerge onto the ground. The female sex pheromone stimulates standby beetles to exhibit emerging and wing vibration behaviours, although the effect depends on when it is presented to beetles. Beetles burrow back into the soil; this behaviour is influenced by illumination and time of the day but not by temperature. The results suggest that D. ishigakiensis possesses a sophisticated mechanism controlling male emergence from the soil.
Journal of Insect Conservation, 2008
... Midori-no-Tokuhon 43:811816 (in Japanese) Someya T (1998) The present state of the damselfly... more ... Midori-no-Tokuhon 43:811816 (in Japanese) Someya T (1998) The present state of the damselfly, Mortonagrion hirosei. Nature Insects 33(10):48 (in Japanese) Southwood TRE, Henderson PA (2000) Ecological methods, 3rd edn. ...
Journal of Ethology, 2012
ABSTRACT In order to understand the visual orientation mechanisms of the male black chafer Holotr... more ABSTRACT In order to understand the visual orientation mechanisms of the male black chafer Holotrichia loochooana loochooana to females, we examined male behavioral response to colored objects (lures) when female sex pheromone was present. In the field, males landed more frequently on darker lures than on lighter ones of the same color. Among lures of different colors but with the same brightness, males preferred landing on black, blue, or red ones to green or grey ones. The reflectance intensities of preferred lures were weaker than those of green and grey in the range of 540–580 nm. This range is considered to affect choice by males. When females perch on the leaf edges of the food plant to adopt a calling posture, the dominant background color is that of the leaves (i.e., green). This means that the landing target forms a silhouette (dark spot) which is prominent against a background of green leaves, providing maximum visual acuity for mating.
Journal of Chemical Ecology, 1996
major component of female contact sex pheromone of the yellow-spotted longicorn beetle, Psacothea... more major component of female contact sex pheromone of the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe), was isolated from the elytra and identified as (Z)-21-methyt-8-pentatriacontene. The synthetic compound released the typical mating behavior incIuding holding, mounting, and abdominal bending in males, although its activity was considerably lower than the extract of female elytra when treated on a gelatin capsule as an artificial female model.