Miladis Camacho - Academia.edu (original) (raw)

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Papers by Miladis Camacho

Current Nutrition & Food Science, 2022

Background: Mushroom-based products can serve as superior nutraceuticals in both preventing and a... more Background: Mushroom-based products can serve as superior nutraceuticals in both preventing and alleviating human diseases. Dehydrated powder of Pleurotus ostreatus grown in Cuba has a rich nutritional composition and a large number of therapeutic properties. However, technological parameters (e.g. rheological), as well as biochemical and microbiological quality control standards for its use as a raw material in the manufacture of solid dosage forms with nutraceutical and biopharmaceutical applications have not been established yet. Objective: The study aims to determine the technological, biochemical and microbiological quality parameters of dehydrated Pleurotus ostreatus powder. Methods: Rheological properties (Carr´s index, Hausner´s ratio, angle of repose and flow rate) were determined in seven batches of mushroom powder. Biochemical and microbiological analyses were performed using standard methods. Other physicochemical properties (mean particle size, pH and moisture) were also determined. Results: Technical parameters showed a mean particle size of 224.08±7.96 µm, Carr´s compressibility index (23.08-26.58%), Hausner´s ratio (1.30-1.36), angle of repose (36.6-41.90°) and flow rate (3.69-6.93 g cm-2 s-1), typical of materials with poor flowability. Biochemical analyses indicated significant nutraceutical composition: protein (25.0-30.4%), carbohydrate (45-60%), ash (6.1-9.8%), fiber (5-9%), β-D-Glucans (1.47-1.61%), iron (21-50 mg/100 g), zinc (3.8-8.0 mg/100 g), and polyphenols (123-160 mg/100 g). Moisture content was ≤3.50% and microbiological analyses were in agreement with ISO standards. Conclusion: Dehydrated P. ostreatus powder exhibits good nutraceutical composition and microbiological quality. Technological parameters evidenced poor flow properties, suggesting the need to include excipients to improve the rheological properties for applications as an active ingredient in solid dosage forms for health benefits.

Summary. Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluy... more Summary. Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianus CCEBI 2011. The aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. Simplex sequential optimization showed that after 15 runs the optimal conditions were: pH, 4.6; temperature, 31ºC; total reducing sugars (TRS), 125 g/l; (NH 4 ) 2 SO 4 , 2.48 g/l; (NH 4 ) 2 HPO 4 , 2.73 g/l; CaCl 2 , 0.33 g/l and MgSO 4 ·7H 2 O, 0.54 g/l. Under these conditions, the ethanol concentration was 47.6 g/l and endoPG concentration was 9.8 U/ml, which represented increases of 22% and 10%, respectively, over the concentrations obtained under suboptimal conditions. Temperature and (NH 4 ) 2 SO 4 supplementation were the most significant factors influencing the co-production process. [Int Microbiol 2...

Innovative Food Science & Emerging Technologies, 2015

ABSTRACT The study examined the in vitro antimicrobial and the complement/macrophage stimulating ... more ABSTRACT The study examined the in vitro antimicrobial and the complement/macrophage stimulating effects of a hot-water extract from mycelium of the oyster mushroom Pleurotus sp. The extract activated the microbial autolytic system of eight strains: seven autolyzing strains with intensity values (IS) ranging from 2.7% in Candida sp. to 36.1% in Saccharomyces cerevisiae, while autolysis was of 1.8% in one non-autolyzing strain (Bacillus cereus). The activation of the alternative pathway of the complement (APC) was dose and time dependent as judged by the lysis of rabbit red blood cells. Two main polysaccharide fractions of the extract significantly bind human immunoglobulin G (IgG) which could result in activation of the classical pathway of the complement (CPC). The extract (5-100 μg/well) enhanced the acid phosphatase activity in murine peritoneal macrophages to 133-184% compared to controls. The findings introduce a novel “bifunctional” approach (antimicrobial-immunomodulatory) to the nutraceutical potential of the Pleurotus hot-water mycelial extract.

International Microbiology, 2011

Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces... more Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianus CCEBI 2011. The aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. Simplex sequential optimization showed that after 15 runs the optimal conditions were: pH, 4.6; temperature, 31ºC; total reducing sugars (TRS), 125 g/l; (NH 4 ) 2 SO 4 , 2.48 g/l; (NH 4 ) 2 HPO 4 , 2.73 g/l; CaCl 2 , 0.33 g/l and MgSO 4 ·7H 2 O, 0.54 g/l. Under these conditions, the ethanol concentration was 47.6 g/l and endoPG concentration was 9.8 U/ml, which represented increases of 22% and 10%, respectively, over the concentrations obtained under suboptimal conditions. Temperature and (NH 4 ) 2 SO 4 supplementation were the most significant factors influencing the co-production process. [Int Microbiol 2011; 14(1):41-49]

Current Nutrition & Food Science, 2022

Background: Mushroom-based products can serve as superior nutraceuticals in both preventing and a... more Background: Mushroom-based products can serve as superior nutraceuticals in both preventing and alleviating human diseases. Dehydrated powder of Pleurotus ostreatus grown in Cuba has a rich nutritional composition and a large number of therapeutic properties. However, technological parameters (e.g. rheological), as well as biochemical and microbiological quality control standards for its use as a raw material in the manufacture of solid dosage forms with nutraceutical and biopharmaceutical applications have not been established yet. Objective: The study aims to determine the technological, biochemical and microbiological quality parameters of dehydrated Pleurotus ostreatus powder. Methods: Rheological properties (Carr´s index, Hausner´s ratio, angle of repose and flow rate) were determined in seven batches of mushroom powder. Biochemical and microbiological analyses were performed using standard methods. Other physicochemical properties (mean particle size, pH and moisture) were also determined. Results: Technical parameters showed a mean particle size of 224.08±7.96 µm, Carr´s compressibility index (23.08-26.58%), Hausner´s ratio (1.30-1.36), angle of repose (36.6-41.90°) and flow rate (3.69-6.93 g cm-2 s-1), typical of materials with poor flowability. Biochemical analyses indicated significant nutraceutical composition: protein (25.0-30.4%), carbohydrate (45-60%), ash (6.1-9.8%), fiber (5-9%), β-D-Glucans (1.47-1.61%), iron (21-50 mg/100 g), zinc (3.8-8.0 mg/100 g), and polyphenols (123-160 mg/100 g). Moisture content was ≤3.50% and microbiological analyses were in agreement with ISO standards. Conclusion: Dehydrated P. ostreatus powder exhibits good nutraceutical composition and microbiological quality. Technological parameters evidenced poor flow properties, suggesting the need to include excipients to improve the rheological properties for applications as an active ingredient in solid dosage forms for health benefits.

Summary. Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluy... more Summary. Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianus CCEBI 2011. The aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. Simplex sequential optimization showed that after 15 runs the optimal conditions were: pH, 4.6; temperature, 31ºC; total reducing sugars (TRS), 125 g/l; (NH 4 ) 2 SO 4 , 2.48 g/l; (NH 4 ) 2 HPO 4 , 2.73 g/l; CaCl 2 , 0.33 g/l and MgSO 4 ·7H 2 O, 0.54 g/l. Under these conditions, the ethanol concentration was 47.6 g/l and endoPG concentration was 9.8 U/ml, which represented increases of 22% and 10%, respectively, over the concentrations obtained under suboptimal conditions. Temperature and (NH 4 ) 2 SO 4 supplementation were the most significant factors influencing the co-production process. [Int Microbiol 2...

Innovative Food Science & Emerging Technologies, 2015

ABSTRACT The study examined the in vitro antimicrobial and the complement/macrophage stimulating ... more ABSTRACT The study examined the in vitro antimicrobial and the complement/macrophage stimulating effects of a hot-water extract from mycelium of the oyster mushroom Pleurotus sp. The extract activated the microbial autolytic system of eight strains: seven autolyzing strains with intensity values (IS) ranging from 2.7% in Candida sp. to 36.1% in Saccharomyces cerevisiae, while autolysis was of 1.8% in one non-autolyzing strain (Bacillus cereus). The activation of the alternative pathway of the complement (APC) was dose and time dependent as judged by the lysis of rabbit red blood cells. Two main polysaccharide fractions of the extract significantly bind human immunoglobulin G (IgG) which could result in activation of the classical pathway of the complement (CPC). The extract (5-100 μg/well) enhanced the acid phosphatase activity in murine peritoneal macrophages to 133-184% compared to controls. The findings introduce a novel “bifunctional” approach (antimicrobial-immunomodulatory) to the nutraceutical potential of the Pleurotus hot-water mycelial extract.

International Microbiology, 2011

Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces... more Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianus CCEBI 2011. The aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. Simplex sequential optimization showed that after 15 runs the optimal conditions were: pH, 4.6; temperature, 31ºC; total reducing sugars (TRS), 125 g/l; (NH 4 ) 2 SO 4 , 2.48 g/l; (NH 4 ) 2 HPO 4 , 2.73 g/l; CaCl 2 , 0.33 g/l and MgSO 4 ·7H 2 O, 0.54 g/l. Under these conditions, the ethanol concentration was 47.6 g/l and endoPG concentration was 9.8 U/ml, which represented increases of 22% and 10%, respectively, over the concentrations obtained under suboptimal conditions. Temperature and (NH 4 ) 2 SO 4 supplementation were the most significant factors influencing the co-production process. [Int Microbiol 2011; 14(1):41-49]