Larry Milner - Academia.edu (original) (raw)

Papers by Larry Milner

Review of Scientific Instruments, Apr 1, 1971

A vacuum manifold apparatus is described which allows rapid ion exchange chromatography of multip... more A vacuum manifold apparatus is described which allows rapid ion exchange chromatography of multiple samples. The apparatus is especially applicable to the assay of enzymes by radioisotopic tracer methods.

Analytical Biochemistry, Oct 1, 1970

American Journal of Cardiology, Apr 1, 1994

Archives of Biochemistry and Biophysics, Jun 1, 1969

A number of compounds whose metabolism might be affected by vitamin Blz were tested for their abi... more A number of compounds whose metabolism might be affected by vitamin Blz were tested for their ability to spare the vitamin requirement for growth in Euglena gracilis. Although none were successful in this regard it was observed that n-methionine, at 2 X 10-S M, completely inhibited growth of the organism in a glutamic acid medium. n-Homocysteine, n-cysteine, and, to a lesser extent, L-threonine at 5 X 10V3M also inhibited growth. This inhibition of growth by n-methionine was not seen when the organism was grown in a medium utilizing acetate as the primary carbon source. Data from radioactive amino acid uptake experiments indicate that L-methionine inhibits the uptake of glutamic acid by the organism.

Proceedings of the National Academy of Sciences of the United States of America, Mar 1, 1970

Phospholipase D (cabbage) inhibits the vectorial phosphorylation of a-methylglucoside by isolated... more Phospholipase D (cabbage) inhibits the vectorial phosphorylation of a-methylglucoside by isolated membrane preparations from Escherichia coli NIL 308-225 without increasing the efflux of intramembranal a-methylglucoside-P. This effect is shown to be related to the ability of phospholipase D to hydrolyze membrane phosphatidylglycerol specifically. After treatment with phospholipase D, the membranes resynthesize phosphatidylglycerol with a return in their ability to take up a-methylglucoside. Since proline uptake by the same preparations is only slightly inhibited by phospholipase D, the data indicate that phosphatidylglycerol is. required specifically for transport processes which are mediated by the P-enolpyruvate-P-transferase system.

Proceedings of the National Academy of Sciences of the United States of America, Jul 1, 1970

The conversion of D-lactate to pyruvate in isolated membrane preparations of E. coli ML 308-225 m... more The conversion of D-lactate to pyruvate in isolated membrane preparations of E. coli ML 308-225 markedly stimulates the transport of proline, glutamic acid, aspartic acid, asparagine, tryptophan, lysine, serine, alanine, and glycine. The uptake of histidine, phenylalanine, tyrosine, leucine, isoleucine, and valine by the membranes is also markedly stimulated by this conversion, although these amino acids are taken up much less effectively than those mentioned previously. The uptake of arginine, methionine, cystine, and cysteine is enhanced only about twofold in the presence of D-(-)-lactate, and these amino acids are not concentrated well by the membranes. With the exception of glutamate, aspartate, asparagine, and methionine, which are converted to other metabolites to varying extents in the intramembranal pool, each of the other amino acids was recovered from the membranes as the unchanged amino acid. Succinate, L-(+)-lactate, D,L-a-hydroxybutyrate, and DPNH partially replace D-(-)-lactate but are less effective. Previous work from this laboratory" 2 demonstrated that isolated membrane preparations from E. coli W6, in the absence of soluble proteins and nucleic acids, catalyzed the concentrative uptake of proline, whereas membranes prepared from E. coli W157, a proline transport mutant,3 did not. Proline uptake by these preparations was stimulated by glucose and was inhibited by anaerobiosis and by a variety of compounds known to uncouple oxidative phosphorylation or inhibit electron transport. Studies recently made with membranes prepared from E. coli 1\IL 308-225 have defined the energetics of the proline uptake system in much greater detail.4 D-(-)-Lactate markedly stimulates proline uptake with a 20to 30-fold increase over baseline levels. Of all the metabolites and cofactors tested, only succinate, L-(+)-lactate, D,L-a-hydroxybutyrate, and DPNH replace D-(-)-lactate to any extent whatsoever. Succinate stimulates proline uptake 8to 10-fold, and L-(+)-lactate, D,L-a-hydroxybutyrate, and DPNH stimulate only 3to 4-10(8

chapter 1 Introduction chapter 2 Historical Background - Antiquity chapter 3 Historical Backgroun... more chapter 1 Introduction chapter 2 Historical Background - Antiquity chapter 3 Historical Background - Influx of Religion chapter 4 Historical Background - Middle Ages chapter 5 Historical Background - Renaissance chapter 6 Historical Background - Modern Times chapter 7 Tribes chapter 8 Exposure chapter 9 Female Infanticide chapter 10 Punishment chapter 11 Shame chapter 12 Sacrifice chapter 13 Poverty & Famine chapter 14 Revenge chapter 15 Money chapter 16 Depression & Insanity chapter 17 Euthanasia chapter 18 Power chapter 19 Twins chapter 20 Love of Another chapter 21 Population Control chapter 22 Infant Image chapter 23 Conclusion chapter 24 Bibliography

Loyola University of Chicago Law Journal, 1987

Association developed a plan recommending, among other items, a federal incentive program to enco... more Association developed a plan recommending, among other items, a federal incentive program to encourage state tort reforms. Under this proposal, states would be given federal grants to help undertake liability reform. Id. 10. Wagner, Liability Insurance Crisis: Coming to Grips With Long Tails and Deep Pockets, 8 ILL. ISSUES 1 (1986). According to the Illinois Coalition on Insurance Crisis, the insurance industry desires changes that will hold down the costs of civil cases, including ceilings on liability awards, limits on contingency fees for plaintiffs' lawyers, and the elimination of joint and several liability. Id. at 5. Organizations supporting limits on damage judgments include the following:

Arch Biochem Biophys, 1969

Review of Scientific Instruments, 1971

Proceedings of the National Academy of Sciences, 1970

Archives of Biochemistry and Biophysics, 1969

The repression of several enzymes involved in methionine synthesis in Escherichia coli by either ... more The repression of several enzymes involved in methionine synthesis in Escherichia coli by either met,hionine or vitamin B,z has been investigated. L-Methionine, at 10m2 M in the growth medium, yielded the following results: 83(y0 repression of the non-Blz t,ransmethylase; 77/j; repression of h '5,'0-methylene-H4-folate reductase; and 75% repression of P-cystathionase. Vitamin B,s, at 10m9 M, showed 99O/o repression of the non-B12 transmethylase, 787, repression of pli '5,'0-methvleIle-Ha-folate reductase, and no repression of p-cystathionase, The significantly higher repression of the non-Blz transmethylase by vitamin B1? when compared to methionine, in conjunction with the lack of p-cystat,hionase repression by the vitamin, suggested that repression by the vitamin was not due to an increase in intracellular methionine formed. This was corroborated by direct, assay of intracellular methionine levels which showed no increase after growth of the organism in the presence of the vitamin. However, the studies with other cobamides suggested that repression of the ~lon-B,n transmethylase by vitamin B12 was dependent on formation of the BI2 transmethylase holoenzymc. These data indicate that the vitamin and methionine may cause repression of the non-Bl? enzyme by different. mechanisms.