Miloud Slimani - Academia.edu (original) (raw)

Papers by Miloud Slimani

Research paper thumbnail of Evaluation De L'Effet Toxique Du Plomb Sur Certains Parametres De Croissance Du Radis " Raphanus Sativus L

Research paper thumbnail of Antimicrobial and Antiochratoxic Activities of Mentha spicata Essential Oil

Phytothérapie, 2021

The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the e... more The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the essential oil of Mentha spicata (EOM). This oil was obtained by hydrodistillation with a yield of 0.3%. EOM was screened for its possible antibacterial activity in vitro against five strains of pathogenic bacteria, using the solid disc diffusion method and the microdilution method. The extract reacted positively to the bacterial strains tested. The results of the antifungal activity show an inhibition of mycelial growth; it is total on Aspergillus ochraceus, Aspergillus flavus, Aspergillus niger, Penicillium viridicatum, and on Rhizopus stolonifer and Alternaria alternate at a concentration of 12.5 μl/ml. Tests carried out on yeasts mainly referenced as Candida albicans IP444 and Candida albicans 2679 showed minimal inhibition (MIC) of the order of 7.81 μl/ml and 0.97 μl/ml, respectively and theMICis of the order of 0.48 μl/ml for Candida albicans 10 231. In addition, the EOM antiochratox...

Research paper thumbnail of Evaluation of anti-oxidative effects of Mentha spicata essential oils in rats at the period of gestation and lactation co-exposed to lead and manganese

South Asian Journal of Experimental Biology, 2019

The mechanisms in the origin of lead and manganese poisoning (Pb-Mn) are multiple, and potentiall... more The mechanisms in the origin of lead and manganese poisoning (Pb-Mn) are multiple, and potentially touch all the body cells. For this purpose, we are interested in oxidative stress induced by Pb-Mn at the erythrocytic level at a dose of 0.2% (Pb) and 4.79 mg/ml (Mn) during the period of gestation and lactation in wistars rats and the ability of mint essential oil, Mentha spicata (MEO) to restore or not this state of stress. The extraction of MEO “HEM” via steam distillation allows us to obtain MEO with an output of 0.49%. Moreo-ver, the analysis of the anti-oxidativeerythrocytic status showed that the co-exposure to Pb-Mn significantly increases enzymes activity of catalase (CAT) and superoxide dismutase (SOD), and significantly reduces the enzyme activity of glutathione peroxidase (GPx), by causing a dysfunction in the anti-oxidative defense system. In contrast, the administration of MEO via intra-peritoneal (IP) during a period of 21 days in the previously Pb-Mn intoxicated rats, ...

Research paper thumbnail of Preventive effect of phytic acid against lead toxicity in growing rats: biochemical assays and measuring of blood and brain lead levels

South Asian Journal of Experimental Biology, 2019

Phytic acid (Inositol hexaphosphate; IP6) is a natural plant antioxidant, it is found in most gra... more Phytic acid (Inositol hexaphosphate; IP6) is a natural plant antioxidant, it is found in most grain and legumes. Although much attention has been focused on the preventive effect during critical periods of development in lead poi-soned rats. Phytic acid (1%) was administered to Wistar rats during the ges-tation period and then exposed them to lead acetate (0.2%) during the lacta-tion period. We observed a decrease in body weight in the lead intoxicated group. Phytic acid treatment reduces blood lead levels and brain lead (P<0.001) compared to untreated poisoned rats. The group exposed to lead and not treated with IP6 showed an increase in liver enzyme activity (ALAT, ASAT and PAL) and electrolyte levels (Na+, K+, Cl¯). While the values of fere-mia and calcium levels decreased compared to those of control rats, batches treated with IP6 showed a significant increase in serum iron and calcium levels, whereas the value of serum Mg+2 was unchanged compared to that of the intoxicated b...

Research paper thumbnail of Viral Infection of the Reproductive System in Times of COVID-19

Handbook of Research on Pathophysiology and Strategies for the Management of COVID-19, 2022

Coronavirus (SARS-COV2) caused several deaths worldwide. This virus infects the target cell by bi... more Coronavirus (SARS-COV2) caused several deaths worldwide. This virus infects the target cell by binding to angiotensin-converting enzymes 2 (ACE2) receptor through its receptor-binding domain (RBD) and replicates. Thus, a high level of ACE2 expression is detected in the testicular cells so that the testis is believed to count as a potential target for direct damage by COVID-19. Moreover, the possibility of testicular damage may be caused by either direct viral invasion through interaction with ACE2 receptors or because of inflammatory response. Similarly, in women, literature reported the distribution and function of ACE2 in the female reproductive system, which is widely expressed in the ovary, uterus, vagina, and placenta. It regulates follicular development and ovulation, modulates luteal angiogenesis and degeneration, and influences regular changes in endometrial tissue and embryo development. Taking these functions into account, COVID-19 may disturb the female reproductive funct...

Research paper thumbnail of Impact of essential oils of clove Syzygium aromaticum in rats exposed to stress by nickel

Notulae Scientia Biologicae, 2020

The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in t... more The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in the body. For this purpose, we were interested in the oxidative stress induced by the Ni at the erythrocyte level at a dose of 2 g/l during the gestation and lactation period in the Wistar rats and the capacity of the clove essential oil, Syzygium aromaticum, (CEO) to restore or not this state of stress. Extraction of the CEO by hydro-distillation allowed us to obtain a CEO with a yield of 10.60% and the characterization of this essential oil by GC-MS indicates that the major components of this oil are: Eugenol (80.95%), eugenyl acetate (10.48%), β-caryophyllene (7.21%) and α-humulene (0.87%). The antioxidant activity of the CEO made in vitro showed a free radical scavenging capacity DPPH with an IC 50 of the order of 25.60 μg/ml. In addition, analysis of the erythrocyte antioxidant status indicated that Ni significantly increased the enzymatic activities of catalase (CAT) and superoxide ...

Research paper thumbnail of Assessment of Oxytetracycline Residue in Cooked and Raw Meat of Chicken Broilers Before and After the End of Official Withdrawal Period

Food Analytical Methods, 2018

The aim of this study was to determine and to compare the presence of oxytetracycline (OTC) resid... more The aim of this study was to determine and to compare the presence of oxytetracycline (OTC) residues in raw and cooked (boiled) meat before and after withdrawal period with the Maximum Residue Limits (MRL) using biological and physicochemical techniques. In animal building of 2000 broiler chickens (Gallus domesticus, breed ISA F15) and under the same conditions, 12 healthy adult chickens were selected and divided in two groups (n = 6 in each group). The sample was treated by Oxytetracycline® 20% (0.4 g/kg) during 3 days. Juices and muscles tissues were sampled twice for this assay: first, the broiler chickens were slaughtered (n = 6) 24 h after the third day of treatment. However, the second sampling (n = 6) was performed 24 h after the end of the withdrawal period. For each portions of sample, the juice meat and muscle tissues samples were collected after cooking at 80 °C during 45 min and after freezing/defrosting for the raw meat. Biological methods consisted of using four plates test (FPT) to pre-screening and Premi®Test to screening. Finally, to confirm and to quantify the accurate level of OTC in positive samples, ultra-high-performance liquid chromatographytandem mass spectrometry (UHPLC-MS/MS) measurements were conducted. The FPT test showed that all samples were positive. While the Premi®Test analysis showed negative results for raw meat tested and positive results for juice of meat cooked in both samples. The obtained results using UHPLC-MS/MS methods confirmed the previously results. Thus, the presence of such toxic substances in our diet constitutes a major health risk for consumers, requiring the establishment of an adequate monitoring system. There is a paucity of official withdrawal periods for veterinary medicinal products in cooking meat; a relationship between the concentrations of residues of antibiotics after waiting period and cooking is not established since meat is always cooked before consumption.

Research paper thumbnail of Neuro beneficial effects of Pimpinella anisum against lead exposure

International Journal of Green Pharmacy, 2013

Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine t... more Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine to treat a variety of diseases, including some neurological disorders. Aims: This study was aimed to test, in vivo, the possible anxiolytic and antidepressant effects, of the essential oil of Pimpinella anisum against chronic lead acetate (0.2%) intoxication during the gestation and lactation period, in Wistar rat pups. Settings and Design: Wistar rat pups were exposed to lead via their dams’ drinking water from postnatal day (PND) 1 to (PND) 21. After weaning, the lead‑exposed rats received injections of essential oil of Pimpinella anisum (0.5 ml/kg) for 15 days. The level of anxiety, depression and locomotor activity were studied. Materials and Methods: The behaviours evaluated were: Locomotor activity (open‑field test), anxiety (dark and light compartment and elevated plus maze tests), and depression (forced swimming test). Statistical Analysis: The data were analysed by two‑way analyses of variance (ANOVAs). When a significant difference was found, the Student‑Newman‑Keuls post‑hoc test was conducted. For all analyses, the difference was considered to be significant at P ≤ 0.05. Results: The results of the present study demonstrate that developmental lead exposure induces, on the one hand, impairments of body (P < 0.001) and brain weight (P < 0.05), respectively, and on the other hand, increases the level of anxiety (P < 0.001), depression (P < 0.001) and locomotor hyperactivity (P < 0.001), compared to control rats. Administration of the essential oil of Pimpinella anisum entrains reduction in the level of anxiety (P < 0.001), depression (P < 0.001) and correct locomotor hyperactivity (P < 0.001) in rats exposed to lead beforehand. Conclusion: In conclusion, our results demonstrate that developmental lead exposure induces significant perturbation of emotional reactivity that can be improved by treatment with the essential oil of Pimpinella anisum. Further evaluation of the use of anise oil in the treatment of neurological disorders is suggested. Key words: Anxiety, depression, lead acetate, locomotor activity, Pimpinella anisum

Research paper thumbnail of Nephroprotective effect of aqueous extract of aerial parts ofHypericum scabrumL

Toxicological & Environmental Chemistry, 2012

Research paper thumbnail of Evaluation of the Toxic Effect of Lead on Some Parameters of Growth Radish Plant (Raphanus sativus L.)

ALGERIAN JOURNAL OF NATURAL PRODUCTS, 2016

The lead pollution has become a serious problem threatening our ecosystems with detrimental effec... more The lead pollution has become a serious problem threatening our ecosystems with detrimental effects on crop production and biodiversity, so much research is currently focused on new environmentally friendly methods such as phytoremediation .The work undertaken registers with a view to study the physiological, biochemical behavior of the plant radish ( Raphanus sativus L. ). In this context, the dose (250, 500 and 1000 mg / l) of lead acetate were applied to radish ( Raphanus sativus L. ) relative to a control (absence of the Lead), for a period of two months. The results show apparent changes in various parameters studied in stressed plants compared to controls. Macroscopic (inhibition of germination rate, decreased biomass and size reduction), physiological (disruption of water status, decreased chlorophyll pigments and carotenoids content) were observed. Histological changes at the level of the rods (deformation of the walls of the medullary parenchyma cells), and roots (occlusion...

Research paper thumbnail of Etudes pharmacologiques et fonctionnelles des systèmes de capture synaptosomaux et vésiculaires des neurones dopaminergiques cérébraux

Http Www Theses Fr, 1988

Internalisation de la dopamine sous differentes conditions experimentales par des vesicules prepa... more Internalisation de la dopamine sous differentes conditions experimentales par des vesicules preparees a partir du striatum de rat. Les differents processus de capture vesiculaires (ATP-Mg dependant, dependant ou independant du delta pH) sont diriges par un meme transporteur sensible a la tetrabenzine mais fonctionnant avec des forces motrices differentes. Etude pharmacologique comparee a revele que les systemes de capture neuronaux et vesiculaires different par leur sensibilite aux agents pharmacologiques. Des derives de la piperazine se comportent comme de puissants inhibiteurs de la capture synapotosomale de la dopamine de GK13 derive de la phencyclidine, inhibe la capture neuronale de la dopamine et induit sur liberation du neuromediateur accumule

Research paper thumbnail of Lead in Water: Neurotoxicityand Stressful Effect on Wistar Rat

The fauna and flora are severely threatened by discharges of industrial pollutants such as heav y... more The fauna and flora are severely threatened by discharges of industrial pollutants such as heav y metals (lead zinc cadmium arsenic…) which are highly dangerous. The study covers the identification of sites fixing mechanism lead to the brain, in rats Wistar according to several approaches: phenotypic, behavioural, immunohistochemical and histological. The rats are permanently acetate lead the dose of 250mg / l and 500mg/l diluted in distilled water for a period of 12 weeks compared to witnesses who receive only distilled water. On the phenotypic we found that in rats treated, lead causes exophthalmoses, a decrease in muscle tone, body weight, the intake and falling hair all over his body: anorectic effect and a decrease in water intake. The administration of the lead acetate oral causes a very significant decrease in the conduct of exploratio n (locomotors activity) and the stereotypical behaviour like (grooming, sniffing bite) compared to control group rats with rats. The experienc...

Research paper thumbnail of Hepatoprotective effects of the decoction and macerated leaves of Rhamnus alaternus L. on rats exposed to carbon tetrachloride

Journal of Pharmacognosy and Phytotherapy, Oct 31, 2015

The plant of Ramnus alaternus L. (Rhamnaceae) has been object of many therapeutic indications for... more The plant of Ramnus alaternus L. (Rhamnaceae) has been object of many therapeutic indications for traditional medicine. Many studies have showed a hepatoprotective activity of R. alaternus L. The objective of this study was to compare the hepatoprotective activities of decoction and macerated R. Alaternus L. leaves extracts on rats initially exposed to carbon tetrachloride CCl 4 (1 ml/kg). The macerated leaves (250 mg/kg body weight) showed a highly significant hepatoprotective activity (p < 0.01) expressed by a significant decrease in enzymatic biochemical markers such as total bilirubin, alkaline phosphatase (ALP) and transaminases (GOT and GPT). Mean serum biochemical marker levels were 4.5 ± 0.9 mg/dL, 143 ± 3.7, 43.5 ± 9.2 and 32.2 ± 5.1 U/L, respectively. These results illustrate the dominance of the hepatoprotective pharmacological activity of macerated R. alaternus L. leaves.

Research paper thumbnail of Evaluation Effect of the Essential Oil of Green Anis (Pimpinella Anisum) on The Liver Function of Young Rats Following Exposure to Mercury Chloride

Egyptian Academic Journal of Biological Sciences. C, Physiology & Molecular Biology, Mar 11, 2023

Research paper thumbnail of Ameliorative Effect of The Essential Oil of Syzygium aromaticum in Wistars Rats Exposed to Aluminum Chloride

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication... more The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication, on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied metal with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Chronic exposure to aluminum chloride at a concentration of 100mg/kg in rats decreased body and organ weights (Liver, Kidney) compared to controls. The intoxication also revealed a disruption of various biochemical parameters, including liver (TGO, TGP), and kidney (urea, creatinine) biochemical assays, Thus, the concentration of aluminium in the intoxicated group is high in the blood. Also, the results show a correction in the values of these parameters following the administration of CEO compared to those of exposed animals. CEO also corrected the shape of the affected blood cells after intoxication to aluminium.

Research paper thumbnail of Neurobenifical Effect of Anised In Rats Exposed to Mercury

Arab Gulf Journal of Scientific Research

Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However,... more Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However, Green anis is a plant widely used in the medical field for its various therapeutic virtues. The aim of this study is to evaluate the effect of subchonic exposure to mercury chloride (HgCl2) on the neurobehavioral status of young wistar rats as well as the neurocorrective effect of essential oil of green anis. Methods: Extraction of essential oil by hydrodisillation. Exposure of developing rats to a dose of (100mg/L). Then, a group of the intoxicated animals underwent therapy with one dose (0.25 ml/kg) for 21 days intraperitoneally. In addition, the realization of a behavior test battery namely; the forced swimming test to assess the state of despair of the pups, the open field to assess the locomotor activity as well as the exploratory capacities followed by a histopathological study of the brain. Data were analyzed by two-way analysis of variance (ANOVA). Results: The results of this s...

Research paper thumbnail of Evaluation of the Therapeutic Effect of Syzygium aromaticum Essential Oil in Wistar Rats Infected with Candida albicans - Biochemical and Hematological Study

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2021

The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infec... more The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infection (10 6 CFU per 0.2 ml of intravenous inoculum) on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied yeast with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Infection of adult rats with Candida albicans by intravenous injection at a concentration of 106 CFU per 0.2 ml of inoculum increased hepatic and renal fungal load. The infection also revealed a disruption of various biochemical parameters, including liver (AST, ALT), kidney (urea, creatinine) biochemical assays, and hematological (white and red blood cells, platelets...); also, the results show a correction in the values of these parameters followi...

Research paper thumbnail of Prophylactic effect of the aqueous extract of Pimpinella anisum on the behavior of Wistar rats exposed to mercury

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2 - exposed rats compared to control rats. However, preventive and curative aniseed-based treatment...

Research paper thumbnail of Neuroprotective effect of Foeniculum vulgare essential oil in developing Wistar rats exposed to manganese chloride

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 2021

The present study was carried out in order to evaluate, on the one hand, the modifications induce... more The present study was carried out in order to evaluate, on the one hand, the modifications induced by manganese chloride according to a neurobehavioral, biochemical and histological approach in developing Wistar rats and, on the other hand, to test the effectiveness of Foeniculum vulgare (fennel) essential oil (FEO) in restoring or not the harmful effects of manganese chloride (MnCl2). The characterization of this essential oil by gas chromatography showed that the major component is E-anethole (34.7907%). The administration of FEO corrected the depressive state, anxiety and locomotor hypoactivity respectively observed in rats exposed to MnCl2, thus, the FEO restored the activity of the various antioxidant enzymes with a clear improvement of the cerebral tissue architecture in intoxicated rats treated with FEO. In conclusion, the FEO has a beneficial effect on the nervous system of rats intoxicated with MnCl2 which justifies the importance of this oil in traditional medicine.

Research paper thumbnail of Behavior and Glutamate Transaminase Changes in Rat Exposed to Lead and Treated by Wormwood Extract

International Journal of Pharmacy and Pharmaceutical Sciences, 2016

Objective : Lead poisoning induced severe behavioral abnormalities and impaired cognitive functio... more Objective : Lead poisoning induced severe behavioral abnormalities and impaired cognitive functions in experimental animals. The aim of the present study is to investigate the detrimental effects of lead exposure on the behavior of rats and its association with altered neurochemistry. Methods : Twenty-four young male Wistar rats were divided into 4 groups: G1: a control group receiving drinking water. G2: intoxicated group (Pb) exposed to lead acetate (1000 ppm in drinking water). G3: receives Wormwood aqueous (A. Ab) extract at a dose of 300 mg/l in drinking water. G4: rats are receiving Pb+A. Ab mixture for 4 additional weeks after intoxication for 8 w. In the present study, locomotors activity in rats was assessed by open field test (OFT) while anxiety and depressive behavior were monitored by elevated plus maze (EPM) and the forced swim test (FST), the evaluation of glutamate metabolizing enzymes in whole brain and lipid peroxidation was carried out in all groups. Results : our ...

Research paper thumbnail of Evaluation De L'Effet Toxique Du Plomb Sur Certains Parametres De Croissance Du Radis " Raphanus Sativus L

Research paper thumbnail of Antimicrobial and Antiochratoxic Activities of Mentha spicata Essential Oil

Phytothérapie, 2021

The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the e... more The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the essential oil of Mentha spicata (EOM). This oil was obtained by hydrodistillation with a yield of 0.3%. EOM was screened for its possible antibacterial activity in vitro against five strains of pathogenic bacteria, using the solid disc diffusion method and the microdilution method. The extract reacted positively to the bacterial strains tested. The results of the antifungal activity show an inhibition of mycelial growth; it is total on Aspergillus ochraceus, Aspergillus flavus, Aspergillus niger, Penicillium viridicatum, and on Rhizopus stolonifer and Alternaria alternate at a concentration of 12.5 μl/ml. Tests carried out on yeasts mainly referenced as Candida albicans IP444 and Candida albicans 2679 showed minimal inhibition (MIC) of the order of 7.81 μl/ml and 0.97 μl/ml, respectively and theMICis of the order of 0.48 μl/ml for Candida albicans 10 231. In addition, the EOM antiochratox...

Research paper thumbnail of Evaluation of anti-oxidative effects of Mentha spicata essential oils in rats at the period of gestation and lactation co-exposed to lead and manganese

South Asian Journal of Experimental Biology, 2019

The mechanisms in the origin of lead and manganese poisoning (Pb-Mn) are multiple, and potentiall... more The mechanisms in the origin of lead and manganese poisoning (Pb-Mn) are multiple, and potentially touch all the body cells. For this purpose, we are interested in oxidative stress induced by Pb-Mn at the erythrocytic level at a dose of 0.2% (Pb) and 4.79 mg/ml (Mn) during the period of gestation and lactation in wistars rats and the ability of mint essential oil, Mentha spicata (MEO) to restore or not this state of stress. The extraction of MEO “HEM” via steam distillation allows us to obtain MEO with an output of 0.49%. Moreo-ver, the analysis of the anti-oxidativeerythrocytic status showed that the co-exposure to Pb-Mn significantly increases enzymes activity of catalase (CAT) and superoxide dismutase (SOD), and significantly reduces the enzyme activity of glutathione peroxidase (GPx), by causing a dysfunction in the anti-oxidative defense system. In contrast, the administration of MEO via intra-peritoneal (IP) during a period of 21 days in the previously Pb-Mn intoxicated rats, ...

Research paper thumbnail of Preventive effect of phytic acid against lead toxicity in growing rats: biochemical assays and measuring of blood and brain lead levels

South Asian Journal of Experimental Biology, 2019

Phytic acid (Inositol hexaphosphate; IP6) is a natural plant antioxidant, it is found in most gra... more Phytic acid (Inositol hexaphosphate; IP6) is a natural plant antioxidant, it is found in most grain and legumes. Although much attention has been focused on the preventive effect during critical periods of development in lead poi-soned rats. Phytic acid (1%) was administered to Wistar rats during the ges-tation period and then exposed them to lead acetate (0.2%) during the lacta-tion period. We observed a decrease in body weight in the lead intoxicated group. Phytic acid treatment reduces blood lead levels and brain lead (P<0.001) compared to untreated poisoned rats. The group exposed to lead and not treated with IP6 showed an increase in liver enzyme activity (ALAT, ASAT and PAL) and electrolyte levels (Na+, K+, Cl¯). While the values of fere-mia and calcium levels decreased compared to those of control rats, batches treated with IP6 showed a significant increase in serum iron and calcium levels, whereas the value of serum Mg+2 was unchanged compared to that of the intoxicated b...

Research paper thumbnail of Viral Infection of the Reproductive System in Times of COVID-19

Handbook of Research on Pathophysiology and Strategies for the Management of COVID-19, 2022

Coronavirus (SARS-COV2) caused several deaths worldwide. This virus infects the target cell by bi... more Coronavirus (SARS-COV2) caused several deaths worldwide. This virus infects the target cell by binding to angiotensin-converting enzymes 2 (ACE2) receptor through its receptor-binding domain (RBD) and replicates. Thus, a high level of ACE2 expression is detected in the testicular cells so that the testis is believed to count as a potential target for direct damage by COVID-19. Moreover, the possibility of testicular damage may be caused by either direct viral invasion through interaction with ACE2 receptors or because of inflammatory response. Similarly, in women, literature reported the distribution and function of ACE2 in the female reproductive system, which is widely expressed in the ovary, uterus, vagina, and placenta. It regulates follicular development and ovulation, modulates luteal angiogenesis and degeneration, and influences regular changes in endometrial tissue and embryo development. Taking these functions into account, COVID-19 may disturb the female reproductive funct...

Research paper thumbnail of Impact of essential oils of clove Syzygium aromaticum in rats exposed to stress by nickel

Notulae Scientia Biologicae, 2020

The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in t... more The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in the body. For this purpose, we were interested in the oxidative stress induced by the Ni at the erythrocyte level at a dose of 2 g/l during the gestation and lactation period in the Wistar rats and the capacity of the clove essential oil, Syzygium aromaticum, (CEO) to restore or not this state of stress. Extraction of the CEO by hydro-distillation allowed us to obtain a CEO with a yield of 10.60% and the characterization of this essential oil by GC-MS indicates that the major components of this oil are: Eugenol (80.95%), eugenyl acetate (10.48%), β-caryophyllene (7.21%) and α-humulene (0.87%). The antioxidant activity of the CEO made in vitro showed a free radical scavenging capacity DPPH with an IC 50 of the order of 25.60 μg/ml. In addition, analysis of the erythrocyte antioxidant status indicated that Ni significantly increased the enzymatic activities of catalase (CAT) and superoxide ...

Research paper thumbnail of Assessment of Oxytetracycline Residue in Cooked and Raw Meat of Chicken Broilers Before and After the End of Official Withdrawal Period

Food Analytical Methods, 2018

The aim of this study was to determine and to compare the presence of oxytetracycline (OTC) resid... more The aim of this study was to determine and to compare the presence of oxytetracycline (OTC) residues in raw and cooked (boiled) meat before and after withdrawal period with the Maximum Residue Limits (MRL) using biological and physicochemical techniques. In animal building of 2000 broiler chickens (Gallus domesticus, breed ISA F15) and under the same conditions, 12 healthy adult chickens were selected and divided in two groups (n = 6 in each group). The sample was treated by Oxytetracycline® 20% (0.4 g/kg) during 3 days. Juices and muscles tissues were sampled twice for this assay: first, the broiler chickens were slaughtered (n = 6) 24 h after the third day of treatment. However, the second sampling (n = 6) was performed 24 h after the end of the withdrawal period. For each portions of sample, the juice meat and muscle tissues samples were collected after cooking at 80 °C during 45 min and after freezing/defrosting for the raw meat. Biological methods consisted of using four plates test (FPT) to pre-screening and Premi®Test to screening. Finally, to confirm and to quantify the accurate level of OTC in positive samples, ultra-high-performance liquid chromatographytandem mass spectrometry (UHPLC-MS/MS) measurements were conducted. The FPT test showed that all samples were positive. While the Premi®Test analysis showed negative results for raw meat tested and positive results for juice of meat cooked in both samples. The obtained results using UHPLC-MS/MS methods confirmed the previously results. Thus, the presence of such toxic substances in our diet constitutes a major health risk for consumers, requiring the establishment of an adequate monitoring system. There is a paucity of official withdrawal periods for veterinary medicinal products in cooking meat; a relationship between the concentrations of residues of antibiotics after waiting period and cooking is not established since meat is always cooked before consumption.

Research paper thumbnail of Neuro beneficial effects of Pimpinella anisum against lead exposure

International Journal of Green Pharmacy, 2013

Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine t... more Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine to treat a variety of diseases, including some neurological disorders. Aims: This study was aimed to test, in vivo, the possible anxiolytic and antidepressant effects, of the essential oil of Pimpinella anisum against chronic lead acetate (0.2%) intoxication during the gestation and lactation period, in Wistar rat pups. Settings and Design: Wistar rat pups were exposed to lead via their dams’ drinking water from postnatal day (PND) 1 to (PND) 21. After weaning, the lead‑exposed rats received injections of essential oil of Pimpinella anisum (0.5 ml/kg) for 15 days. The level of anxiety, depression and locomotor activity were studied. Materials and Methods: The behaviours evaluated were: Locomotor activity (open‑field test), anxiety (dark and light compartment and elevated plus maze tests), and depression (forced swimming test). Statistical Analysis: The data were analysed by two‑way analyses of variance (ANOVAs). When a significant difference was found, the Student‑Newman‑Keuls post‑hoc test was conducted. For all analyses, the difference was considered to be significant at P ≤ 0.05. Results: The results of the present study demonstrate that developmental lead exposure induces, on the one hand, impairments of body (P < 0.001) and brain weight (P < 0.05), respectively, and on the other hand, increases the level of anxiety (P < 0.001), depression (P < 0.001) and locomotor hyperactivity (P < 0.001), compared to control rats. Administration of the essential oil of Pimpinella anisum entrains reduction in the level of anxiety (P < 0.001), depression (P < 0.001) and correct locomotor hyperactivity (P < 0.001) in rats exposed to lead beforehand. Conclusion: In conclusion, our results demonstrate that developmental lead exposure induces significant perturbation of emotional reactivity that can be improved by treatment with the essential oil of Pimpinella anisum. Further evaluation of the use of anise oil in the treatment of neurological disorders is suggested. Key words: Anxiety, depression, lead acetate, locomotor activity, Pimpinella anisum

Research paper thumbnail of Nephroprotective effect of aqueous extract of aerial parts ofHypericum scabrumL

Toxicological & Environmental Chemistry, 2012

Research paper thumbnail of Evaluation of the Toxic Effect of Lead on Some Parameters of Growth Radish Plant (Raphanus sativus L.)

ALGERIAN JOURNAL OF NATURAL PRODUCTS, 2016

The lead pollution has become a serious problem threatening our ecosystems with detrimental effec... more The lead pollution has become a serious problem threatening our ecosystems with detrimental effects on crop production and biodiversity, so much research is currently focused on new environmentally friendly methods such as phytoremediation .The work undertaken registers with a view to study the physiological, biochemical behavior of the plant radish ( Raphanus sativus L. ). In this context, the dose (250, 500 and 1000 mg / l) of lead acetate were applied to radish ( Raphanus sativus L. ) relative to a control (absence of the Lead), for a period of two months. The results show apparent changes in various parameters studied in stressed plants compared to controls. Macroscopic (inhibition of germination rate, decreased biomass and size reduction), physiological (disruption of water status, decreased chlorophyll pigments and carotenoids content) were observed. Histological changes at the level of the rods (deformation of the walls of the medullary parenchyma cells), and roots (occlusion...

Research paper thumbnail of Etudes pharmacologiques et fonctionnelles des systèmes de capture synaptosomaux et vésiculaires des neurones dopaminergiques cérébraux

Http Www Theses Fr, 1988

Internalisation de la dopamine sous differentes conditions experimentales par des vesicules prepa... more Internalisation de la dopamine sous differentes conditions experimentales par des vesicules preparees a partir du striatum de rat. Les differents processus de capture vesiculaires (ATP-Mg dependant, dependant ou independant du delta pH) sont diriges par un meme transporteur sensible a la tetrabenzine mais fonctionnant avec des forces motrices differentes. Etude pharmacologique comparee a revele que les systemes de capture neuronaux et vesiculaires different par leur sensibilite aux agents pharmacologiques. Des derives de la piperazine se comportent comme de puissants inhibiteurs de la capture synapotosomale de la dopamine de GK13 derive de la phencyclidine, inhibe la capture neuronale de la dopamine et induit sur liberation du neuromediateur accumule

Research paper thumbnail of Lead in Water: Neurotoxicityand Stressful Effect on Wistar Rat

The fauna and flora are severely threatened by discharges of industrial pollutants such as heav y... more The fauna and flora are severely threatened by discharges of industrial pollutants such as heav y metals (lead zinc cadmium arsenic…) which are highly dangerous. The study covers the identification of sites fixing mechanism lead to the brain, in rats Wistar according to several approaches: phenotypic, behavioural, immunohistochemical and histological. The rats are permanently acetate lead the dose of 250mg / l and 500mg/l diluted in distilled water for a period of 12 weeks compared to witnesses who receive only distilled water. On the phenotypic we found that in rats treated, lead causes exophthalmoses, a decrease in muscle tone, body weight, the intake and falling hair all over his body: anorectic effect and a decrease in water intake. The administration of the lead acetate oral causes a very significant decrease in the conduct of exploratio n (locomotors activity) and the stereotypical behaviour like (grooming, sniffing bite) compared to control group rats with rats. The experienc...

Research paper thumbnail of Hepatoprotective effects of the decoction and macerated leaves of Rhamnus alaternus L. on rats exposed to carbon tetrachloride

Journal of Pharmacognosy and Phytotherapy, Oct 31, 2015

The plant of Ramnus alaternus L. (Rhamnaceae) has been object of many therapeutic indications for... more The plant of Ramnus alaternus L. (Rhamnaceae) has been object of many therapeutic indications for traditional medicine. Many studies have showed a hepatoprotective activity of R. alaternus L. The objective of this study was to compare the hepatoprotective activities of decoction and macerated R. Alaternus L. leaves extracts on rats initially exposed to carbon tetrachloride CCl 4 (1 ml/kg). The macerated leaves (250 mg/kg body weight) showed a highly significant hepatoprotective activity (p < 0.01) expressed by a significant decrease in enzymatic biochemical markers such as total bilirubin, alkaline phosphatase (ALP) and transaminases (GOT and GPT). Mean serum biochemical marker levels were 4.5 ± 0.9 mg/dL, 143 ± 3.7, 43.5 ± 9.2 and 32.2 ± 5.1 U/L, respectively. These results illustrate the dominance of the hepatoprotective pharmacological activity of macerated R. alaternus L. leaves.

Research paper thumbnail of Evaluation Effect of the Essential Oil of Green Anis (Pimpinella Anisum) on The Liver Function of Young Rats Following Exposure to Mercury Chloride

Egyptian Academic Journal of Biological Sciences. C, Physiology & Molecular Biology, Mar 11, 2023

Research paper thumbnail of Ameliorative Effect of The Essential Oil of Syzygium aromaticum in Wistars Rats Exposed to Aluminum Chloride

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication... more The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication, on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied metal with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Chronic exposure to aluminum chloride at a concentration of 100mg/kg in rats decreased body and organ weights (Liver, Kidney) compared to controls. The intoxication also revealed a disruption of various biochemical parameters, including liver (TGO, TGP), and kidney (urea, creatinine) biochemical assays, Thus, the concentration of aluminium in the intoxicated group is high in the blood. Also, the results show a correction in the values of these parameters following the administration of CEO compared to those of exposed animals. CEO also corrected the shape of the affected blood cells after intoxication to aluminium.

Research paper thumbnail of Neurobenifical Effect of Anised In Rats Exposed to Mercury

Arab Gulf Journal of Scientific Research

Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However,... more Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However, Green anis is a plant widely used in the medical field for its various therapeutic virtues. The aim of this study is to evaluate the effect of subchonic exposure to mercury chloride (HgCl2) on the neurobehavioral status of young wistar rats as well as the neurocorrective effect of essential oil of green anis. Methods: Extraction of essential oil by hydrodisillation. Exposure of developing rats to a dose of (100mg/L). Then, a group of the intoxicated animals underwent therapy with one dose (0.25 ml/kg) for 21 days intraperitoneally. In addition, the realization of a behavior test battery namely; the forced swimming test to assess the state of despair of the pups, the open field to assess the locomotor activity as well as the exploratory capacities followed by a histopathological study of the brain. Data were analyzed by two-way analysis of variance (ANOVA). Results: The results of this s...

Research paper thumbnail of Evaluation of the Therapeutic Effect of Syzygium aromaticum Essential Oil in Wistar Rats Infected with Candida albicans - Biochemical and Hematological Study

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2021

The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infec... more The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infection (10 6 CFU per 0.2 ml of intravenous inoculum) on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied yeast with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Infection of adult rats with Candida albicans by intravenous injection at a concentration of 106 CFU per 0.2 ml of inoculum increased hepatic and renal fungal load. The infection also revealed a disruption of various biochemical parameters, including liver (AST, ALT), kidney (urea, creatinine) biochemical assays, and hematological (white and red blood cells, platelets...); also, the results show a correction in the values of these parameters followi...

Research paper thumbnail of Prophylactic effect of the aqueous extract of Pimpinella anisum on the behavior of Wistar rats exposed to mercury

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2 - exposed rats compared to control rats. However, preventive and curative aniseed-based treatment...

Research paper thumbnail of Neuroprotective effect of Foeniculum vulgare essential oil in developing Wistar rats exposed to manganese chloride

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 2021

The present study was carried out in order to evaluate, on the one hand, the modifications induce... more The present study was carried out in order to evaluate, on the one hand, the modifications induced by manganese chloride according to a neurobehavioral, biochemical and histological approach in developing Wistar rats and, on the other hand, to test the effectiveness of Foeniculum vulgare (fennel) essential oil (FEO) in restoring or not the harmful effects of manganese chloride (MnCl2). The characterization of this essential oil by gas chromatography showed that the major component is E-anethole (34.7907%). The administration of FEO corrected the depressive state, anxiety and locomotor hypoactivity respectively observed in rats exposed to MnCl2, thus, the FEO restored the activity of the various antioxidant enzymes with a clear improvement of the cerebral tissue architecture in intoxicated rats treated with FEO. In conclusion, the FEO has a beneficial effect on the nervous system of rats intoxicated with MnCl2 which justifies the importance of this oil in traditional medicine.

Research paper thumbnail of Behavior and Glutamate Transaminase Changes in Rat Exposed to Lead and Treated by Wormwood Extract

International Journal of Pharmacy and Pharmaceutical Sciences, 2016

Objective : Lead poisoning induced severe behavioral abnormalities and impaired cognitive functio... more Objective : Lead poisoning induced severe behavioral abnormalities and impaired cognitive functions in experimental animals. The aim of the present study is to investigate the detrimental effects of lead exposure on the behavior of rats and its association with altered neurochemistry. Methods : Twenty-four young male Wistar rats were divided into 4 groups: G1: a control group receiving drinking water. G2: intoxicated group (Pb) exposed to lead acetate (1000 ppm in drinking water). G3: receives Wormwood aqueous (A. Ab) extract at a dose of 300 mg/l in drinking water. G4: rats are receiving Pb+A. Ab mixture for 4 additional weeks after intoxication for 8 w. In the present study, locomotors activity in rats was assessed by open field test (OFT) while anxiety and depressive behavior were monitored by elevated plus maze (EPM) and the forced swim test (FST), the evaluation of glutamate metabolizing enzymes in whole brain and lipid peroxidation was carried out in all groups. Results : our ...