Mine Taskin - Academia.edu (original) (raw)

Papers by Mine Taskin

The aim of the study is to investigate the effects of the interleukin-6 (IL-6) blocker tocilizuma... more The aim of the study is to investigate the effects of the interleukin-6 (IL-6) blocker tocilizumab in a hyperstimulated rat model and compare it with ranibizumab, a gonadotropin-releasing hormone antagonist (GnRHA), and cabergoline. Forty-seven rats were randomly divided into the following seven groups: Group 1: OHS; Group 2: OHS+ GnRHA; Group 3: OHS + ranibizumab; Group 4: OHS + cabergoline; Group 5: OHS + low-dose tocilizumab (TL); Group 6: OHS + high-dose tocilizumab (TH); Group 7: sham. Ovarian weight was significantly lower only in the ranibizumab group than in the OHS group. Estrogen levels were significantly lower in the GnRHA group than in the OHS and the treatment groups. Progesterone levels were significantly lower in the ranibizumab, cabergoline, and TL groups than in the OHS group. Among the treatment groups, corpus luteum counts were lower than in the OHS group. Corpus luteum counts were lowest in the tocilizumab groups. IL-6 intensity was lower in all treatment groups ...

Gynecology Obstetrics and Reproductive Medicine, 2016

Endometriosis is a benign, estrogen-dependent, chronic gynecological disorder commonly associated... more Endometriosis is a benign, estrogen-dependent, chronic gynecological disorder commonly associated with pelvic pain and infertility. The prevalence of pelvic endometriosis is high, affecting approximately 6- 10% of women of reproductive age. Although endometriosis has been associated with the occurence of menstrual cycles, it may affect between 2-5% of postmenopausal women. In this presentation, we would like to draw attention to such cases of postmenopausal endometriosis. A 56-year-old woman was presented with postmenopausal vaginal bleeding. Her menopausal status occured at 46 years of age. She had neither personal history of endometriosis and a prior history of pelvic pain nor previous hormone use. A pelvic ultrasound scan revealed a right ovarian cystic mass of approximately 2.6x2.1cm in size and a left ovarian multiple lobated hemorrhagic cystic mass approximately 5.0x2.8 cm in size. Cancer antigen 25 (CA 125) level appeared to be within the normal range. The patient was then su...

uterine rupture in early pregnancy is a very rare complication and it occurs usually in scarred u... more uterine rupture in early pregnancy is a very rare complication and it occurs usually in scarred uteri. Here we present a case of spontaneous uterine rupture at 13 weeks of gestation in a 32-year-old woman. Our preoperative diagnosis was also uterine rupture. Emergency laparotomy confirmed our initial diagnosis. Clinical signs of uterine rupture in early pregnancy are nonspecific and must be distinguished from those of acute abdominal emergencies, espe - cially ectopic pregnancy. Although spontaneous uterine rupture is thought to be a life-threatening obstetrical emergency carrying a high risk for the mother and the fetus because of hemoperitoneum risk, herein we report a patient with a 13 week missed pregnancy after uterine rupture without serious bleeding.

Lenfanjiomlar lenfatik sistemin benign proliferasyonlaridir; endotelle cevrili dilate lenf kanall... more Lenfanjiomlar lenfatik sistemin benign proliferasyonlaridir; endotelle cevrili dilate lenf kanallarindan olusan yuzeyel bir vaskuler malformasyondur. Lenfanjiomlarin 3 tipi vardir: sirkumskriptum, kavernoz ve kistik. Lenfanjioma sirkumskriptum (LS), kutanoz lenfanjiomlarin en sik gorulenidir, konjenital olabilecegi gibi, lenfatik akimin bozulmasina bagli eriskin yasta da ortaya cikabilir. En fazla gorulen lokalizasyonlar aksilla, oral kavite, ust ekstremiteler, omuzlar, kasik, makat, gibi lenfatik akimi yogun olan bolgelerdir. Vulva oldukca nadir gorulen tutulum bolgelerinden biridir. Makroskobik gorunumu ince duvarli, gruplar halinde, degisik boyutlardaki vezikuler gorunumlu lezyonlarla karakterizedir. Alt ekstremitede asimetrik odemin eslik ettigi vakalar da literaturde bulunmaktadir. Mikroskobisinde dermiste, lenfatik damarlarin proteinoz sivi ile dolu duzensiz kistik dilatasyonlari ve proliferasyonlari izlenir. Tanida biopsi altin standarttir. Ayirici tanida vulvar herpes zoster...

INTRODUCTION Studies investigating the relationship between sex hormones, inflammatory mediators ... more INTRODUCTION Studies investigating the relationship between sex hormones, inflammatory mediators and joint disorders have reported that sex hormones affect the pathogenesis of joint disorders. We aimed to investigate temporomandibular joint disorder (TJD) in polycystic ovary syndrome (PCOS) and the possible role of systemic mediators and sex hormones in TJD pathogenesis. MATERIAL AND METHODS PCOS patients (n = 45) and controls (n = 30) aged 20-40 years, were enrolled in this case-control study. TJD diagnosis was performed by the specialist and blood samples were tested in the early follicular phase and on the 21 st (midluteal) day to investigate the levels of estrogen, progesterone, matrix metalloproteinase (MMP) 1-8-9, interleukin (IL)-1ß and Tumor necrosis factor (TNF)-α. RESULTS TJD incidence was significantly higher in PCOS than the control group (51.1% and 6.9% respectively, p < 0.01). Midluteal progesterone (p < 0.01) was lower in PCOS group than the controls (p < 0.0...

Special hormonal and immunological changes are required for normal pregnancy continuation. To esc... more Special hormonal and immunological changes are required for normal pregnancy continuation. To escape from rejection by the maternal immune system, pregnancy needs an optimum environment with the integration and the balance of immune factors. As an immunologically unique site that permits allogenic fetus to be tolerated by mother, the maternal–fetal interface has a vital role. Microorganisms may trigger innate immune responses at the maternal–fetal interface and this may have a significant impact on the success of pregnancy. While the presence of inflammatory markers are slightly increased in healthy pregnancies, their significant increase in preeclampsia suggests that the balance between the inflammatory and antiinflammatory mechanisms may be disrupted by a shift towards inflammation. Based on these immunological observations, we aimed to review the literature for the link between the inflammatory response and preeclampsia since its etiology has not yet been clarified.

Women with polycystic ovary syndrome (PCOS) are more likely to suffer from obesity, insulin resis... more Women with polycystic ovary syndrome (PCOS) are more likely to suffer from obesity, insulin resistance, and chronic low-grade inflammation than other women. Cyclophilin A (CyPA) is known as an inflammatory mediator that is secreted by various types of cells in response to inflammatory stimuli. Previous studies have shown that immunohistochemical expressions and/or circulating levels of CyPA are high in many diseases that cause inflammatory conditions in the body. This study aimed to evaluate serum levels of CyPA and their correlation with clinical and biochemical parameters of women with PCOS. In the study population, we analyzed 49 consecutive patients with PCOS and 30 age and body mass index (BMI)-matched non-PCOS healthy volunteers (Control group). PCOS was diagnosed using Rotterdam criteria. Serum CypA levels were measured using a CyPA ELISA Kit. The relationship between serum CyPA levels and the clinicopathological variables of PCOS were also evaluated. Average levels of CyPA w...

Therapeutic Advances in Endocrinology and Metabolism

Background: Polycystic ovary syndrome is known to be the most common hormonal disorder in women o... more Background: Polycystic ovary syndrome is known to be the most common hormonal disorder in women of reproductive age. Current evidence shows that regulatory proteins secreted from the adipose tissue called adipokines may have a role in polycystic ovary syndrome. We planned to investigate the role of endotrophin that has never been researched in polycystic ovary syndrome before and its correlation with other metabolic parameters and adipokines such as adiponectin and ghrelin in patients with polycystic ovary syndrome. Methods: Forty-three women ( n: 43) with polycystic ovary syndrome and 43 ( n: 43) women as a control group were enrolled in this cross-sectional study. Serum levels of endotrophin, adiponectin, and ghrelin levels were measured with the enzyme-linked immunosorbent assay method. High-density lipoprotein cholesterol, low-density lipoprotein cholesterol, total cholesterol levels, luteinizing hormone/follicle-stimulating hormone ratio, total testosterone, and triglyceride le...

Journal of Obstetrics and Gynaecology

Abstract The chronic course of endometriosis suggests that the immune system may play a role in i... more Abstract The chronic course of endometriosis suggests that the immune system may play a role in its aetiology. There may be resistance to cell lysis, as well as an immune defect underlying endometriosis. Granzyme B is a serine protease that is secreted by Natural Killer (NK) cells and cytotoxic T lymphocytes during a cellular immune response and can induce apoptosis. The aim of this study was to evaluate the relationship between both Granzyme B levels and Granzyme B gene polymorphisms in endometriosis patients. Women between the ages of 20 – 45 were included in the study. The patients were divided into two groups: those diagnosed with endometriosis and those who had not been diagnosed with endometriosis. In the blood samples, Granzyme B gene polymorphisms and serum levels of Granzyme B were studied. There was no difference between the groups in terms of median Granzyme B levels and the presence of AA, AG, and GG genotypes. There was a difference in median granzyme levels for the control group; the GG genotype was found at a lower frequency. The immune defect within endometriosis-related immune cells may not be exclusively due to Granzyme B. Other mediators that are secreted from immune cells may have additive effects. IMPACT STATEMENT What is already known on this subject? NK cells are cytotoxic and inhibit the implantation of autologous endometrial cells that are spilled into the peritoneum by retrograde menstruation. Thus, a reduction in NK cell activity may facilitate the progression of endometriosis. The literature review reveals that there are studies suggesting that NK cell activity may be insufficient in endometriosis. Granzyme B is a serine protease that is secreted by NK cells and cytotoxic T lymphocytes during a cellular immune response. What do the results of this study add? Granzyme B is one of the cytotoxic granules in NK and cytotoxic T lymphocyte cells and its genetic polymorphisms were tested in endometriosis. We found that median Granzyme B levels were significantly different in patients with the GG genotype in the control group, compared to those with the AA and AG genotype. However, this difference was not detected between the control and endometriosis groups. What are the implications of these findings for clinical practice and/or further research? Our results contribute to uncovering the pathogenesis of endometriosis since there are no previous studies in the literature regarding this topic. Although we did not find a difference, our results will inform further studies made on this topic. Studies with different molecules and an increased number of patients are needed. The immune defect of endometriosis may not be due exclusively to Granzyme B. Other mediators that are secreted from immune cells may have mutual effects and interactions.

Medicine Science | International Medical Journal, 2017

Russell bodies are prominent eosinophilic inclusions seen in the cytoplasm of plasma cells and so... more Russell bodies are prominent eosinophilic inclusions seen in the cytoplasm of plasma cells and sometimes outside the cell. There are very few publications available in the English literature on Russell Body Cervicitis. Our case is a 40 year old female patient with a positive screening test for HPV DNA. The cervical surface epithelium in biopsy specimens obtained from the cervix completely have eroded intense vesicular eccentric nuclei and large eosinophilic cytoplasm containing focal Russell Body. The infiltration of dense plasma cells were observed. Our case is the fourth case in the English literature. The case is rare and the etiology has not yet been elucidated.

Advances in Clinical and Experimental Medicine

Background. Apart from the role of progesterone in reproductive physiology, the protective role o... more Background. Apart from the role of progesterone in reproductive physiology, the protective role of exogenously administered progesterone was observed in various injuries, such as neurologic defects and acute kidney injury. Objectives. The aim of the present study was to investigate the effects of progesterone therapy on the immunoexpression of anti-Müllerian hormone (AMH) and the number of apoptotic cells in ovarian damage induced with cisplatin, a chemotherapeutic agent, in an experimental rat model. Material and methods. Forty rats were randomly divided into 4 groups; the control group (the saline group), the cisplatin-treated group (rats were injected with 5 mg/kg/week cisplatin intraperitoneally (i.p.)), the cisplatin + progesterone-treated group (the rats were pretreated with 8 mg/kg progesterone intramuscularly (i.m.) (8 mg/kg) before they were injected with 5 mg/kg/week cisplatin i.p.), and the progesteronetreated group (the rats were treated with 8 mg/kg progesterone i.m.). The ovaries were removed from the rats in all groups 5 days after the final injection of cisplatin. Results. Histopathologic examination and follicle counting were performed. The immunoreactivity intensity of AMH and apoptosis were compared. Histological analysis of the ovaries treated with cisplatin showed ovarian damage. Immunohistochemical analysis showed that the immunoreactivity intensity of AMH, a biomarker that discriminates the degree of ovarian damage, was lower in the cisplatin-treated groups than in other groups. Terminal deoxynucleotide transferase-mediated 20-deoxyuridine 50-triphosphate nick endlabeling (TUNEL) assays showed that the increase in the number of apoptotic cells was statistically significant in the cisplatin-treated group compared to the control group (p < 0.05). Progesterone administration with cisplatin resulted in decreases in TUNEL-positive cells. The decrease in the number of apoptotic cells was statistically significant in the cisplatin + progesterone-treated group compared to the control group (p < 0.001). Conclusions. Our results showed that using progesterone as an adjuvant agent against ovarian damage in patients undergoing cancer chemotherapy with cisplatin is beneficial.

Journal of Obstetrics and Gynaecology, 2016

Polycystic ovary syndrome (PCOS) is a multifactorial disease characterised by chronic inflammatio... more Polycystic ovary syndrome (PCOS) is a multifactorial disease characterised by chronic inflammation. We aimed to investigate an association between IL-1A and IL-6 gene polymorphisms and both hormonal/biochemical parameters and levels of IL-1A and IL-6. A total of 103 women diagnosed with PCOS according to ESHRE/ASRM criteria were investigated. The patients were divided into two groups as obese and non-obese. IL-1A and IL-6 genes polymorphisms as well as hormonal/biochemical parameters and levels of IL-1A and IL-6 were analysed in the same groups. Serum IL-1A and IL-6 levels were found to increase both in obese and non-obese groups. However, there was no association between IL-1A level and IL-1A polymorphism. A relationship was detected between H score, FSH, LH, total testosterone, HDL-C and TG levels and CG + GG genotypes of IL-6. Furthermore, an association was found between IL-6 levels and CC genotype of IL-6 in the obese PCOS patients. The abnormalities in hormonal/biochemical parameters detected in Turkish PCOS patients may be related with IL-6 gene polymorphism rather than IL-1A.

Cukurova Medical Journal, 2016

Öz Endometriosis that is defined as the presence of functional endometrial tissue outside the ute... more Öz Endometriosis that is defined as the presence of functional endometrial tissue outside the uterine cavity is seen 5-10% of reproductive age women. Endometriosis can be seen

Balıkesır Health Sciences Journal, 2015

Reproductive Sciences, 2015

The aim of this study was to investigate the efficacy of anti-interleukin 6 (IL-6) therapy in the... more The aim of this study was to investigate the efficacy of anti-interleukin 6 (IL-6) therapy in the treatment of endometriosis in a rat model. After the peritoneal implantation of autologous endometrial tissue, 22 Wistar female rats were divided to create 2 intervention groups: the tocilizumab group (n = 13) and the control group (n = 9). After measuring implant volume, saline was administered to the rats in the control group and 8 mg/kg tocilizumab was administered intraperitoneally to the rats in the tocilizumab-treated group every 2 weeks. After a 4-week treatment period, the volumes and histopathological properties of the implants were evaluated. A scoring system was used to evaluate the preservation of epithelia. Fibrosis score was assessed between the groups. Ectopic and eutopic endometrium were evaluated immunohistochemically for IL-6 and vascular endothelial growth factor (VEGF). There was a significant difference between the volumes of implants before and after treatment in the tocilizumab group (P &amp;lt; .05). The posttreatment volumes of lesions were smaller in the tocilizumab group than in the control group. Histologic and fibrosis scores were lower in the tocilizumab group than in the control group. Immunoreactivity intensity for VEGF was significantly decreased in the tocilizumab group for ectopic and eutopic endometrium (P &amp;lt; .05). Interleukin 6 levels and endometrial thickness for ectopic and eutopic endometrium were similar between the groups. Tocilizumab treatment had a regressive effect on the endometriotic implants.

Pakistan Journal of Medical Sciences, 1969

Round ligament varicosity (RLV) is rare and almost all cases are pregnant women. RLV appears as a... more Round ligament varicosity (RLV) is rare and almost all cases are pregnant women. RLV appears as a unilateral or bilateral groin swelling. Pain and tenderness may present. Clinical evaluation is inadequate for exact diagnosis because inguinal hernia has similar findings. Ultrasonography (US) is essential when a groin swelling is detected in a pregnant woman. We present gray scale US and colour Doppler US findings of a 32week pregnant woman with bilateral RLVs at the inguinal canal, parauterine area and in the myometrium.

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, Jan 15, 2015

Nesfatin-1, encoded by the nucleobindin-2 (NUCB2) gene, is an anorexigenic protein related to ene... more Nesfatin-1, encoded by the nucleobindin-2 (NUCB2) gene, is an anorexigenic protein related to energy metabolism, obesity, and insulin resistance. The aim of this study was to evaluate NUCB2 gene polymorphism (rs757081) and its association with serum levels of nesfatin-1 in obese and non-obese women with polycystic ovary syndrome (PCOS). In the study population, we analyzed 60 patients with PCOS and 26 age-matched healthy women as controls. The patients with PCOS were divided into two groups based on body mass index (BMI): obese group (n = 28) or non-obese group (n = 32). NUCB2 was genotyped using the polymerase chain reaction-restriction (PCR) technique. Serum nesfatin-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Nesfatin-1 levels in the obese PCOS group were significantly lower than those in the non-obese PCOS and control groups (p < 0.001). There was no statistically significant difference in the distribution of NUCB2 genotypes among the groups (p > 0.0...

Journal of the Chinese Medical Association, 2015

Background: Carbonic anhydrases (CAs), a group of metalloenzymes, are involved in numerous physio... more Background: Carbonic anhydrases (CAs), a group of metalloenzymes, are involved in numerous physiological and pathological processes such as acidebase balance, gluconeogenesis, lipogenesis, ureagenesis, electrolyte secretion in various tissues, bone resorption and calcification, and tumorigenicity. In the current study, we aimed to determine and compare possible alterations in the activity of carbonic anhydrase I (CA I) and carbonic anhydrase II (CA II) isozymes by using estrogens and progestagens in female smokers and nonsmokers. Methods: Blood samples from 30 smoker and 30 nonsmoker volunteers were drawn after obtaining informed consent. The blood samples were centrifuged to separate the plasma and erythrocytes. Thereafter, hemolysate was prepared from the red cells. CA I and CA II were purified from human erythrocytes with a simple one-step procedure using Sepharose 4B-l-tyrosine-sulfonamide affinity column. CAI and CA II isozymes were treated with estrogen and progesterone-containing drugs, after which the inhibition or activation of the enzyme was determined. Results: CA I and CA II enzyme activity was observed to be increased in female smokers. The results of this study show that dienogest is the most effective inhibitor for human erythrocytes CA I when compared with micronized progesterone, hydroxyprogesterone caproate, estradiol valerate, and estradiol hemihydrate in both female smokers and nonsmokers. All active ingredients have been shown to have a stronger inhibition in smokers than nonsmokers for CA I activity. Additionally, estradiol valerate and hydroxyprogesterone caproate have stronger inhibition against CA II enzyme activity in women who smoke. Conclusion: The results of the current study provide important information to clinicians about how to consider the possible adverse effects of these drugs which are produced as a result of inhibition of CA I and CA II enzyme. Clinicians should take into consideration the side effects caused by CA I and CA II enzyme inhibition when prescribing these drugs in the treatment of different clinical conditions, especially in women who smoke.

International Journal of Surgery Case Reports, 2015

INTRODUCTION: Accessory Spleen (AS) is a very rare entity and usually near the spleen's hilum and... more INTRODUCTION: Accessory Spleen (AS) is a very rare entity and usually near the spleen's hilum and in the tail of the pancreas. Pelvis reported as an atypical and a rare localization. AS may be formed during embryonic life, they rise from the left side of the dorsal mesogastrium as a result of imperfect fusion of separate splenic masses. PRESENTATION OF CASE: We report a case of an AS presenting as an left adnexal mass in a middle-aged woman. Transvaginal ultrasonography and magnetic resonance imaging (MRI) revealed a left adnexial mass. Laparatomy was performed, and histological examination revealed that resected mass was splenic tissue. DISCUSSION: An AS is an incidental finding of no clinical significance in most patients. AS are generally determined during radiological investigations or during open or laparoscopic surgeries. When, the AS settle in the adnexal area; the differential diagnosis could include the causes of adnexal masses like enlarged lymph nodes, subserous fibroid, ovarian tumors, organized hematoma, tuboovarian abscess. CONCLUSION: Althought pelvic accessory spleen is a rare condition, should be considered in the differential diagnosis of adnexal masses.

European journal of obstetrics, gynecology, and reproductive biology, 2014

To determine the effects of different intra-abdominal pressure values on visceral pain following ... more To determine the effects of different intra-abdominal pressure values on visceral pain following gynecologic laparoscopic surgery in the Trendelenburg position. This randomized, controlled prospective trial was conducted at a tertiary education hospital and included 150 patients who underwent gynecologic laparoscopy with different abdominal insufflation pressures. There were 54 patients in the 8 mmHg low pressure group (LPG), 45 in the 12 mmHg standard pressure group (SPG), and 51 in the 15 mmHg high pressure group (HPG). We assessed mean age, body mass index (BMI), duration of surgery, analgesic consumption, length of hospital stay, amount of CO2 expended and volume of hemorrhage. Visceral pain and referred visceral pain were assessed 6, 12, and 24 h postoperatively using a visual analog scale (VAS). There was no significant difference in age, BMI, analgesic consumption or length of hospital stay among groups. The mean operative time and total CO2 expended during surgery were highe...