Mirza Hasnain - Academia.edu (original) (raw)

Papers by Mirza Hasnain

Research paper thumbnail of Usefulness of Serial Multiorgan Point-of-Care Ultrasound in Acute Heart Failure: Results from a Prospective Observational Cohort

Background and Objectives: Acute Heart Failure (AHF) is a common disease and a cause of high morb... more Background and Objectives: Acute Heart Failure (AHF) is a common disease and a cause of high morbidity and mortality, constituting a major health problem. The main purpose of this study was to determine the impact of multiorgan ultrasound in identifying the pulmonary hypertension (PH) in patients admitted due to AHF, predict the evolution of the disease during hospitalization and identify areas of improvement in the care of patients with AHF. Materials and Methods: Patients were evaluated with a standard exam of lung ultrasound, echocardiography, inferior vena cava (IVC) and femoral, renal, hepatic, portal venous Doppler flow patterns at admission and on the day of discharge. Results: Thirty patients were enrolled during November 2021. The mean age was seventy-nine years (Standard Deviation – SD 13,4). Seven patients (23.3%) had a renal function worsening. Regarding ultrasound findings, venous excess ultrasonography score (VExUS) score was calculated at admission and at discharge, s...

Research paper thumbnail of Next-generation sequencing in clinical virology: A review of its applications

Pure and Applied Biology, 2020

Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detectio... more Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detection of infection-causing pathogens. Owing to the increasing demand for cost-effective and quick DNA sequencing protocols, many research groups have reported the use of NGS for lab research and experimentation. NGS has revolutionized the field of DNA sequencing by helping sequence big data and overcomes the limitations associated with Sangers sequencing. In the current manuscript, we have aimed to review the feasibility of using NGS in public and clinical health laboratories for disease diagnostics. We believe that this review will add to the available literature by highlighting the important aspects of NGS in the characterization of various pathogens which cannot be grown using culture media and other conventional approaches.

Research paper thumbnail of A Modified Method for the Quantitative Estimation of Diphenyl in Citrus Fruits

Journal of AOAC INTERNATIONAL, 1957

Во трудот поимот социјален работник се однесува на сите профили вработени во социјалните институц... more Во трудот поимот социјален работник се однесува на сите профили вработени во социјалните институции, што е практика во многу земји.

Research paper thumbnail of Computational Analysis of Functional Single Nucleotide Polymorphisms Associated with the CYP11B2 Gene

PLoS ONE, 2014

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variations in humans a... more Single nucleotide polymorphisms (SNPs) are the most common type of genetic variations in humans and play a major role in the genetics of human phenotype variation and the genetic basis of human complex diseases. Recently, there is considerable interest in understanding the possible role of the CYP11B2 gene with corticosterone methyl oxidase deficiency, primary aldosteronism, and cardio-cerebro-vascular diseases. Hence, the elucidation of the function and molecular dynamic behavior of CYP11B2 mutations is crucial in current genomics. In this study, we investigated the pathogenic effect of 51 nsSNPs and 26 UTR SNPs in the CYP11B2 gene through computational platforms. Using a combination of SIFT, PolyPhen, I-Mutant Suite, and ConSurf server, four nsSNPs (F487V, V129M, T498A, and V403E) were identified to potentially affect the structure, function, and activity of the CYP11B2 protein. Furthermore, molecular dynamics simulation and structure analyses also confirmed the impact of these nsSNPs on the stability and secondary properties of the CYP11B2 protein. Additionally, utilizing the UTRscan, MirSNP, PolymiRTS and miRNASNP, three SNPs in the 39UTR region were predicted to exhibit a pattern change in the upstream open reading frames (uORF), and eight microRNA binding sites were found to be highly affected due to 39UTR SNPs. This cataloguing of deleterious SNPs is essential for narrowing down the number of CYP11B2 mutations to be screened in genetic association studies and for a better understanding of the functional and structural aspects of the CYP11B2 protein.

Research paper thumbnail of Maximum power point tracking of partially shaded solar photovoltaic arrays

Solar Energy Materials and Solar Cells, 2010

The paper presents the simulation and hardware implementation of maximum power point (MPP) tracki... more The paper presents the simulation and hardware implementation of maximum power point (MPP) tracking of a partially shaded solar photovoltaic (PV) array using a variant of Particle Swarm Optimization known as Adaptive Perceptive Particle Swarm Optimization (APPSO). Under partially shaded conditions, the photovoltaic (PV) array characteristics get more complex with multiple maxima in the power-voltage characteristic. The paper presents an algorithmic technique to accurately track the maximum power point (MPP) of a PV array using an APPSO. The APPSO algorithm has also been validated in the current work. The proposed technique uses only one pair of sensors to control multiple PV arrays. This result in lower cost and higher accuracy of 97.7% compared to earlier obtained accuracy of 96.41% using Particle Swarm Optimization. The proposed tracking technique has been mapped onto a MSP430FG4618 microcontroller for tracking and control purposes. The whole system based on the proposed has been realized on a standard two stage power electronic system configuration.

Research paper thumbnail of Insilico modeling and analysis of small molecules binding to the PHLPP1 protein by molecular dynamics simulation

Pakistan journal of pharmaceutical sciences, 2020

The PHLPP (Pleckstrin homology domain leucine-rich repeat protein phosphatases) is a newly discov... more The PHLPP (Pleckstrin homology domain leucine-rich repeat protein phosphatases) is a newly discovered group of genes which includes PHLPP1 and PHLPP2 and plays an integral part in several cellular processes like apoptosis, cell signaling cell survival, and cell proliferation etc. Both the activation and deactivation of these genes can have vital role in several ailments like heart diseases, circadian rhythm and most importantly the cancer, hence encouraging the growth of novel therapeutic elements. To give new directions into the development of PHLPP1- targeting drugs, the interaction mechanism between PHLPP1 and five important ligands 4IP, B39, 635, ATP and GTA were investigated through docking and Molecular Dynamics Simulation. It is also noteworthy to be mentioned here that there is no previous crystal structure of PHLPP1 available. The in-silico results can provide potential base for advancements in development of new therapeutic elements targeting different diseases, mainly can...

Research paper thumbnail of Usefulness of Serial Multiorgan Point-of-Care Ultrasound in Acute Heart Failure: Results from a Prospective Observational Cohort

Background and Objectives: Acute Heart Failure (AHF) is a common disease and a cause of high morb... more Background and Objectives: Acute Heart Failure (AHF) is a common disease and a cause of high morbidity and mortality, constituting a major health problem. The main purpose of this study was to determine the impact of multiorgan ultrasound in identifying the pulmonary hypertension (PH) in patients admitted due to AHF, predict the evolution of the disease during hospitalization and identify areas of improvement in the care of patients with AHF. Materials and Methods: Patients were evaluated with a standard exam of lung ultrasound, echocardiography, inferior vena cava (IVC) and femoral, renal, hepatic, portal venous Doppler flow patterns at admission and on the day of discharge. Results: Thirty patients were enrolled during November 2021. The mean age was seventy-nine years (Standard Deviation – SD 13,4). Seven patients (23.3%) had a renal function worsening. Regarding ultrasound findings, venous excess ultrasonography score (VExUS) score was calculated at admission and at discharge, s...

Research paper thumbnail of Next-generation sequencing in clinical virology: A review of its applications

Pure and Applied Biology, 2020

Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detectio... more Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detection of infection-causing pathogens. Owing to the increasing demand for cost-effective and quick DNA sequencing protocols, many research groups have reported the use of NGS for lab research and experimentation. NGS has revolutionized the field of DNA sequencing by helping sequence big data and overcomes the limitations associated with Sangers sequencing. In the current manuscript, we have aimed to review the feasibility of using NGS in public and clinical health laboratories for disease diagnostics. We believe that this review will add to the available literature by highlighting the important aspects of NGS in the characterization of various pathogens which cannot be grown using culture media and other conventional approaches.

Research paper thumbnail of A Modified Method for the Quantitative Estimation of Diphenyl in Citrus Fruits

Journal of AOAC INTERNATIONAL, 1957

Во трудот поимот социјален работник се однесува на сите профили вработени во социјалните институц... more Во трудот поимот социјален работник се однесува на сите профили вработени во социјалните институции, што е практика во многу земји.

Research paper thumbnail of Computational Analysis of Functional Single Nucleotide Polymorphisms Associated with the CYP11B2 Gene

PLoS ONE, 2014

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variations in humans a... more Single nucleotide polymorphisms (SNPs) are the most common type of genetic variations in humans and play a major role in the genetics of human phenotype variation and the genetic basis of human complex diseases. Recently, there is considerable interest in understanding the possible role of the CYP11B2 gene with corticosterone methyl oxidase deficiency, primary aldosteronism, and cardio-cerebro-vascular diseases. Hence, the elucidation of the function and molecular dynamic behavior of CYP11B2 mutations is crucial in current genomics. In this study, we investigated the pathogenic effect of 51 nsSNPs and 26 UTR SNPs in the CYP11B2 gene through computational platforms. Using a combination of SIFT, PolyPhen, I-Mutant Suite, and ConSurf server, four nsSNPs (F487V, V129M, T498A, and V403E) were identified to potentially affect the structure, function, and activity of the CYP11B2 protein. Furthermore, molecular dynamics simulation and structure analyses also confirmed the impact of these nsSNPs on the stability and secondary properties of the CYP11B2 protein. Additionally, utilizing the UTRscan, MirSNP, PolymiRTS and miRNASNP, three SNPs in the 39UTR region were predicted to exhibit a pattern change in the upstream open reading frames (uORF), and eight microRNA binding sites were found to be highly affected due to 39UTR SNPs. This cataloguing of deleterious SNPs is essential for narrowing down the number of CYP11B2 mutations to be screened in genetic association studies and for a better understanding of the functional and structural aspects of the CYP11B2 protein.

Research paper thumbnail of Maximum power point tracking of partially shaded solar photovoltaic arrays

Solar Energy Materials and Solar Cells, 2010

The paper presents the simulation and hardware implementation of maximum power point (MPP) tracki... more The paper presents the simulation and hardware implementation of maximum power point (MPP) tracking of a partially shaded solar photovoltaic (PV) array using a variant of Particle Swarm Optimization known as Adaptive Perceptive Particle Swarm Optimization (APPSO). Under partially shaded conditions, the photovoltaic (PV) array characteristics get more complex with multiple maxima in the power-voltage characteristic. The paper presents an algorithmic technique to accurately track the maximum power point (MPP) of a PV array using an APPSO. The APPSO algorithm has also been validated in the current work. The proposed technique uses only one pair of sensors to control multiple PV arrays. This result in lower cost and higher accuracy of 97.7% compared to earlier obtained accuracy of 96.41% using Particle Swarm Optimization. The proposed tracking technique has been mapped onto a MSP430FG4618 microcontroller for tracking and control purposes. The whole system based on the proposed has been realized on a standard two stage power electronic system configuration.

Research paper thumbnail of Insilico modeling and analysis of small molecules binding to the PHLPP1 protein by molecular dynamics simulation

Pakistan journal of pharmaceutical sciences, 2020

The PHLPP (Pleckstrin homology domain leucine-rich repeat protein phosphatases) is a newly discov... more The PHLPP (Pleckstrin homology domain leucine-rich repeat protein phosphatases) is a newly discovered group of genes which includes PHLPP1 and PHLPP2 and plays an integral part in several cellular processes like apoptosis, cell signaling cell survival, and cell proliferation etc. Both the activation and deactivation of these genes can have vital role in several ailments like heart diseases, circadian rhythm and most importantly the cancer, hence encouraging the growth of novel therapeutic elements. To give new directions into the development of PHLPP1- targeting drugs, the interaction mechanism between PHLPP1 and five important ligands 4IP, B39, 635, ATP and GTA were investigated through docking and Molecular Dynamics Simulation. It is also noteworthy to be mentioned here that there is no previous crystal structure of PHLPP1 available. The in-silico results can provide potential base for advancements in development of new therapeutic elements targeting different diseases, mainly can...