Mitchell Gershten MD - Academia.edu (original) (raw)
Papers by Mitchell Gershten MD
Journal of Nervous and Mental Disease, May 1, 2003
... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. ... more ... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. Papakostas, and David ... Effect Management, and Drug-Drug Interactions with Natural Psychotropic Medications and Acupuncture 321 David Mischoulon and Christina M. Dording ...
Life Sciences, 1983
3H-Dopamine (DA) accumulation by storage vesicles from whole rat brain was significantly stablize... more 3H-Dopamine (DA) accumulation by storage vesicles from whole rat brain was significantly stablized in a buffer system based upon the membrane-impermeant D-potassium tartrate. 3H-DA uptake saturated by twenty minutes (Km 2.1 X 10(-5)M) and remained stable for periods of 40-60 minutes. Accumulated DA was rapidly exchangeable with exogenous DA. Total levels of accumulation (pmol/mg protein) were 41.7 +/- 2.9 (37 degrees), 11.9 +/- 2.5 (4 degrees), 31.3 +/- 1.8 (absence of ATP), 26.3 +/- 2.7 (reserpine, 10(-6)M), 26.1 +/- 0.67 (no ATP + reserpine 10(-6), and 14.6 +/- 2.4 (carbonylcyanide-p-triflouromethoxyphenylhydrazone, FCCP, 10(-6)M). Depletion of endogenous DA levels by pretreatment of the animals with alpha-methyl-p-tyrosine greatly diminished the reserpine-insensitive DA accumulation. After depletion of endogenous DA, ATP-independent uptake was significantly retarded, but eventually reached near-control levels. This uptake was abolished in the presence of FCCP (10(-6)M). The results suggest that endogenous levels of DA and ATP contribute to the reserpine- and ATP-insensitive DA accumulation observed in vesicles from untreated animals. HPLC analysis demonstrated no conversion of DA to norepinephrine (NE) in the course of the experiments.
The Journal of Nervous and Mental Disease, 2003
... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. ... more ... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. Papakostas, and David ... Effect Management, and Drug-Drug Interactions with Natural Psychotropic Medications and Acupuncture 321 David Mischoulon and Christina M. Dording ...
![Research paper thumbnail of Uptake of L-[3H] glutamic acid by crude and purified synaptic vesicles from rat brain](https://attachments.academia-assets.com/72424045/thumbnails/1.jpg)
](Biochemical and Biophysical Research Communications, 1982
Rat brain synaptic vesicles suspended in a yiun crnnprised of potassium tartrate displayed satura... more Rat brain synaptic vesicles suspended in a yiun crnnprised of potassium tartrate displayed saturable accumulation of L[ H] glutamic acid at 37" (Km 2.0 x 10JtM; 311*13 pmol/mg protein), 60 min. which was stable for periods up to The accumulation was temperature sensitive and partially ATP-dependent, uptake levels being reduced to 18.7tO.8 ~pnol/xg protein at 4", and to 14124 pnol/nq protein in the absence of ATP. Fractionation of a crude vesicle preparation on a discontinuous sucrose gradient demonstrated the accumulation to be specifically associated with the synaptic vesicle fraction.
Journal of Nervous and Mental Disease, May 1, 2003
... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. ... more ... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. Papakostas, and David ... Effect Management, and Drug-Drug Interactions with Natural Psychotropic Medications and Acupuncture 321 David Mischoulon and Christina M. Dording ...
Life Sciences, 1983
3H-Dopamine (DA) accumulation by storage vesicles from whole rat brain was significantly stablize... more 3H-Dopamine (DA) accumulation by storage vesicles from whole rat brain was significantly stablized in a buffer system based upon the membrane-impermeant D-potassium tartrate. 3H-DA uptake saturated by twenty minutes (Km 2.1 X 10(-5)M) and remained stable for periods of 40-60 minutes. Accumulated DA was rapidly exchangeable with exogenous DA. Total levels of accumulation (pmol/mg protein) were 41.7 +/- 2.9 (37 degrees), 11.9 +/- 2.5 (4 degrees), 31.3 +/- 1.8 (absence of ATP), 26.3 +/- 2.7 (reserpine, 10(-6)M), 26.1 +/- 0.67 (no ATP + reserpine 10(-6), and 14.6 +/- 2.4 (carbonylcyanide-p-triflouromethoxyphenylhydrazone, FCCP, 10(-6)M). Depletion of endogenous DA levels by pretreatment of the animals with alpha-methyl-p-tyrosine greatly diminished the reserpine-insensitive DA accumulation. After depletion of endogenous DA, ATP-independent uptake was significantly retarded, but eventually reached near-control levels. This uptake was abolished in the presence of FCCP (10(-6)M). The results suggest that endogenous levels of DA and ATP contribute to the reserpine- and ATP-insensitive DA accumulation observed in vesicles from untreated animals. HPLC analysis demonstrated no conversion of DA to norepinephrine (NE) in the course of the experiments.
The Journal of Nervous and Mental Disease, 2003
... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. ... more ... of Depression: Roles of S-Adenosyl-I.-Methionine and Folate 68 Jonathan E. Alpees, George I. Papakostas, and David ... Effect Management, and Drug-Drug Interactions with Natural Psychotropic Medications and Acupuncture 321 David Mischoulon and Christina M. Dording ...
Biochemical and Biophysical Research Communications, 1982
Rat brain synaptic vesicles suspended in a yiun crnnprised of potassium tartrate displayed satura... more Rat brain synaptic vesicles suspended in a yiun crnnprised of potassium tartrate displayed saturable accumulation of L[ H] glutamic acid at 37" (Km 2.0 x 10JtM; 311*13 pmol/mg protein), 60 min. which was stable for periods up to The accumulation was temperature sensitive and partially ATP-dependent, uptake levels being reduced to 18.7tO.8 ~pnol/xg protein at 4", and to 14124 pnol/nq protein in the absence of ATP. Fractionation of a crude vesicle preparation on a discontinuous sucrose gradient demonstrated the accumulation to be specifically associated with the synaptic vesicle fraction.