Mitsuo Sekikawa - Academia.edu (original) (raw)

Papers by Mitsuo Sekikawa

Journal of Nutritional Science and Vitaminology, 2005

We examined the effects of Amylomyces rouxii, which is a mold found in some fermented foods in In... more We examined the effects of Amylomyces rouxii, which is a mold found in some fermented foods in Indonesia, on serum cholesterol and hepatic LDL receptor mRNA in rats. Rats were fed a 0.5% cholesterol-enriched diet with (A. rouxii group) or without (control group) 30 g/kg A. rouxii for 4 wk. There were no significant differences in the body weight, food intake or liver weight among the groups. However, the weight of the cecum in the A. rouxii-fed group was significantly higher than that in the control group. The cecal pH in the A. rouxii-fed group was significantly lower than that in the control group. Cecal acetic acid, propionic acid and total SCFA concentrations in the A. rouxii-fed group were significantly higher than those in the control group. The serum total cholesterol and VLDL+intermediate density lipoprotein (IDL)+LDL-cholesterol concentrations in the control group were significantly higher than those in the A. rouxii-fed group at the end of the 4-wk feeding period. There were no significant differences in the HDL-cholesterol or triglyceride concentrations between the groups. The hepatic LDL receptor and cholesterol 7alpha-hydroxylase mRNA levels in the A. rouxii-fed group were significantly higher than those in the control group. The results of this study demonstrate that feeding of A. rouxii lowers the serum total cholesterol level by enhancement of the cecal SCFA concentration and the hepatic LDL receptor mRNA.

Food Science and Biotechnology, Jun 1, 2007

Nihon Chikusan Gakkaiho, 2004

$Research paper thumbnail of Resistant starch fraction prepared from kintoki bean affects gene expression of genes associated with cholesterol metabolism in rats$

Experimental Biology and Medicine, Sep 1, 2004

Feeding rats beans with resistant starch reduces the serum cholesterol concentration; however, th... more Feeding rats beans with resistant starch reduces the serum cholesterol concentration; however, the mechanism is not fully understood. We examined the effects of resistant starch of kintoki (Phaseolus vulgaris, variety) bean on serum cholesterol and hepatic mRNAs in rats. Male F344/Du Crj rats were fed a cholesterol-free diet either with 5 g of cellulose powder (control)/100 g or 5 g of pancreatin-resistant fraction prepared from kintoki bean (kintoki)/100 g diet for 4 weeks. There were no differences in the body weight gain, food intake, liver weight, and mass of cecum contents between the groups. Serum total cholesterol, very low density lipoprotein (VLDL) + intermediate density lipoprotein (IDL) + low density lipoprotein (LDL)-cholesterol, and high density lipoprotein (HDL)-cholesterol levels in the kintoki group were significantly (at least P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) lower than in the control group throughout the feeding period. There was no difference in the serum triglyceride concentration between two groups throughout the feeding period. Total hepatic cholesterol in the control group was significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01) lower than in the kintoki groups. Fecal bile acid, cecal acetate, propionate and n-butyrate concentrations in the kintoki group all were significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) higher than in the control group. Likewise, hepatic cholesterol 7alpha-hydroxylase, LDL receptor, and SR-B1 mRNA levels in the kintoki group were significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) higher than in the control group. The results suggest that resistant starch of kintoki bean reduces serum cholesterol level by increasing hepatic LDL receptor, SR-B1, and cholesterol 7alpha-hydroxylase mRNAs.

Nihon Chikusan Gakkaiho, 2005

Journal of Nutritional Science and Vitaminology, 2007

We examined the effects of different types of buckwheat sprouts on the plasma cholesterol concent... more We examined the effects of different types of buckwheat sprouts on the plasma cholesterol concentration, fecal steroid excretion and hepatic mRNA expression related to cholesterol metabolism in rats. Rats were fed a cholesterol-free diet with 5 g of Kitawasesoba common buckwheat sprout powder (KS)/100 g, 5 g of Hokkai T no. 8 tartary buckwheat sprout powder (HS-8)/100 g or 5 g of Hokkai T no. 9 tartary buckwheat sprout powder (HS-9)/100 g of diet for 4 wk. Control rats were fed a diet with alpha-cornstarch instead of sprout powder for 4 wk. There were no significant differences in food intake, body weight, liver weight or cecal contents among the groups. Plasma total cholesterol concentrations in the HS-8 and HS-9 groups were significantly lower than in the control group, whereas there was no significant difference between the KS and control groups. Fecal bile acid excretion and cecal short-chain fatty acid concentrations in the KS, HS-8 and HS-9 groups were significantly greater than in the control group. Furthermore, fecal matter excretion in the KS, HS-8 and HS-9 groups tended to be increased compared to the control group, with that in the HS-8 group being significantly higher than in the control group. Hepatic cholesterol 7alpha-hydroxylase mRNA expression in the KS, HS-8 and HS-9 groups and hepatic HMG-CoA reductase mRNA expression in the HS-9 group were significantly higher than in the control group. The results suggest that tartary buckwheat sprout powder has a serum cholesterol-lowering function by enhancing fecal bile acid excretion through increased fecal matter excretion or the upregulation of hepatic cholesterol 7alpha-hydroxylase mRNA expression in rats.

Bioscience Biotechnology and Biochemistry, Jun 1, 2007

We examined the effects of red potato flakes (RPF) on serum antioxidant potential and hepatic mRN... more We examined the effects of red potato flakes (RPF) on serum antioxidant potential and hepatic mRNA in rats. The serum thiobarbituric acid-reactive substances concentration and hepatic superoxide dismutase mRNA level in rats fed RPF were significantly lower and higher respectively than those in control rats. These results suggest that RPF might improve the antioxidant system by enhancing hepatic SOD mRNA.

Nihon Chikusan Gakkaiho, 2005

Http Dx Doi Org 10 1271 Bbb 60097, May 22, 2014

The protective effects of red potato extract (RPE) as to liver damage were determined in D-galact... more The protective effects of red potato extract (RPE) as to liver damage were determined in D-galactosamine (GalN)-intoxicated rats. Increases in serum aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase activities, all of which were induced by GalN injection, decreased in RPE administered rats, suggesting that RPE acts as a functional food showing anti-hepatotoxicity.

Bioscience Biotechnology and Biochemistry, Jul 23, 2011

The Journal of Anthropological Society of Nippon, 1990

ABSTRACT The occlusal surfaces of deciduous upper second molars were photographed by moire contou... more ABSTRACT The occlusal surfaces of deciduous upper second molars were photographed by moire contourography using the standard trigonal plane. The ridges of a cusp, omprising a central ridge and the mesial and distal accessory ridges, were identified by the patterns of the moire fringes. The central ridge was present on all cusps except for the hypocone in both sexes. Frequencies of the two accessory ridges on trigonal cusps exceeded 60% except for the distal accessory ridge on the etacone. The mesial accessory ridge on the metacone was significantly more common in females than in males, but the running pattern of the cuspal ridges showed little difference between sexes. The oblique ridge of the deciduous molar was well developed compared with that of the permanent first molar. This ridge was observed in all subjects, but its height and structural components varied remarkably. A small tubercle on the oblique ridge was sometimes observed, usually located on the metacone side. This tubercle was similar to the metaconule in its position and morphology. Intercuspal distances showed larger mean values in the molars with a metaconule than those without. This implied that the overall crown size was affected by the degree of development of some crown components, such as the ridges and tubercles.

Jinruigaku zasshi. The Journal of the Anthropological Society of Nippon

The Journal of Anthropological Society of Nippon, 1985

ABSTRACT Cusp areas of the mandibular first molar were measured in five groups of three racial po... more ABSTRACT Cusp areas of the mandibular first molar were measured in five groups of three racial populations: Japanese male and female, Dutch, and Australian Aboriginals male and female. Total values of five cusp areas were the largest in Aboriginals, 114.9mm2, and the smallest in Dutch, 96.1 mm2. Percent cusp areas (% of total area) do not show population differences. However, there was a tendency that the proportion of these five areas in Aboriginals approximates to those of fossil hominids having large teeth. Correlations between percent cusp areas and the total value show deviations from isometry to allometry. Then, correlation coefficients, regression slope and standard error of slope were obtained from log cusp areas and log total area in order to obtain allometric coefficients. Results of the test for isometry of regression slope show that hypoconid except for Dutch, and protoconid and metaconid except for Dutch and Japanese female have significant positive and negative allometry, respectively. The reason why Japanese female and Dutch do not show allometric trend may due to small size of the tooth and low frequency of 6th cusp. If the statistical allometric trend in the present study is reasonable analogues for allometric relationship within the fossil hominid taxa, the hypothetical reduction of the trigonid in the 'robust' Australopithecus may be an allometric phenomenon.

Japanese Journal of Oral Biology, 1988

ABSTRACT The occlusal surfaces of deciduous upper second molars in the Japanese population were m... more ABSTRACT The occlusal surfaces of deciduous upper second molars in the Japanese population were measured with the aid of moiré contourography. The mean values of the cusp heights and intercuspal distances were larger in females than in males, although these differences were insignificant except for the distance between the paracone and metacone. When the results obtained were compared with those permanent first molars, the crown size was smaller in deciduous than in permanent molars, especially. the height of the hypocone and the intercuspal distances in the buccolingual direction. Low correlations between the cusp heights and the intercusal distances in the permanent molara were also found in deciduous molars, suggesting that the cusp height of the human deciduous molar is independent from the transverse size of the crown.

Journal of Oleo Science, 2005

Nihon Chikusan Gakkaiho, 2005

$Research paper thumbnail of Resistant starch fraction prepared from kintoki bean affects gene expression of genes associated with cholesterol metabolism in rats$

Experimental biology and medicine (Maywood, N.J.), 2004

British Journal of Nutrition, 2006

Anthocyanins from various vegetables and fruits have antioxidant activities, however, the bioacti... more Anthocyanins from various vegetables and fruits have antioxidant activities, however, the bioactivities of coloured potato anthocyanins are not well studied. We examined the antioxidant capacities of pigmented fractions from purple potato flakes in vitro, and the antioxidant potentials of purple potato flakes in vivo. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of the pigmented fraction from Hokkai no. 92 (H92) potato flakes was higher than that from Kitamurasaki (KM) potato flakes. Extracts equivalent to 600 microg pigmented fractions from KM and H92 potato flakes inhibited linoleic acid oxidation in the order trolox&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;H92&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; or =KM&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;control. Rats were fed 25% KM or H92 potato flake diets for 4 weeks. The major anthocyanin was identified as petanin. Control rats were fed a diet with cornstarch instead of potato flakes for 4 weeks. The serum antioxidant potential level in the H92 group was significantly higher than that in the control group. The degree of hepatic lipid peroxidation in the H92 group was significantly lower than that in the control group. Hepatic Cu/Zn-superoxide dismutase (SOD), Mn-SOD and glutathione peroxidase (GSH-Px) mRNA levels in the H92 group were significantly higher than those in the control group. Similar significant differences in Cu/Zn-SOD and Mn-SOD mRNA levels between the KM and control groups were found. The present results suggest that purple potato flakes have antioxidant functions with regard to radical scavenging activity and inhibition of linoleic acid oxidation, and that they improve the antioxidant potentials in rats by enhancing hepatic Mn-SOD, Cu/Zn-SOD and GSH-Px mRNA expression.