Mladen Boban - Academia.edu (original) (raw)

Papers by Mladen Boban

Journal of Clinical Medicine

COVID-19-associated vascular disease complications are primarily associated with endothelial dysf... more COVID-19-associated vascular disease complications are primarily associated with endothelial dysfunction; however, the consequences of disease on vascular structure and function, particularly in the long term (>7 weeks post-infection), remain unexplored. Individual pre- and post-infection changes in arterial stiffness as well as central and systemic hemodynamic parameters were measured in patients diagnosed with mild COVID-19. As part of in-laboratory observational studies, baseline measurements were taken up to two years before, whereas the post-infection measurements were made 2–3 months after the onset of COVID-19. We used the same measurement protocol throughout the study as well as linear and mixed-effects regression models to analyze the data. Patients (N = 32) were predominantly healthy and young (mean age ± SD: 36.6 ± 12.6). We found that various parameters of arterial stiffness and central hemodynamics—cfPWV, AIx@HR75, and cDBP as well as DBP and MAP—responded to a mild ...

Frontiers in Cardiovascular Medicine

BackgroundLarge longitudinal studies with repeated pulse wave velocity (PWV) measurements, a dire... more BackgroundLarge longitudinal studies with repeated pulse wave velocity (PWV) measurements, a direct measure of arterial stiffness, are required to realize the full potential of arterial stiffness in clinical practice. To facilitate such studies it is important to increase the power of a study by reducing within-subject variability of PWV, and to ease the use of a PWV device in clinical settings by minimizing PWV measurement difficulties.MethodsWe systematically investigated experimental setting and meteorological conditions, as well as physiological factors and participant characteristics, to determine whether and to what extent they affected: between- and within-subjects variability of PWV recordings, and measurement difficulties of a particular device. We conducted a 2-week longitudinal block-randomized cross-over study with two blinded observers and two commonly used devices: applanation tonometry SphygmoCor CvMS and oscillometric Arteriograph to assess carotid-femoral (cfPWV) or...

Analizom 128 povijesti bolesti radnika tvornice "Jugovinil" koji su u desetogodisnjem r... more Analizom 128 povijesti bolesti radnika tvornice "Jugovinil" koji su u desetogodisnjem razdoblju lijeceni na Klinici za unutarnje bolesti KBC "Firule" - Split, utvrđeno je da je njih 32 (25%) lijeceno zbog hepatopatije, a po svojoj je prilici posljedica hepatotokickog ucinka vinilklorid monomera (VCM), kome su ovi radnici nerijetko eksponirani u visokoj koncentraciji. Prikazani su histoloski nalazi biopsije koji se povezuje s VCM ostecenjem: toksicni hepatitis fibroza, ciroza, angiosarkom i hemangiopericitom. Posebna je pažnja posvecena ovim posljednjim entitetima: angiosarkomu zbog vrlo nepovoljne prognoze, a hemangiopericitomu jos zbog toga sto je njegova povezanost s ekspozicijom VCM-u sada jos prvi put uocena.

We tested the effects of some phenolic acid derivatives of red wine on the contraction of rat aor... more We tested the effects of some phenolic acid derivatives of red wine on the contraction of rat aortic and porcine coronary rings. Wine as a mixture of different drugs relaxes all blood vessels, and most of the substances used in our study relax the isolated vessels, too.

The content of Pharmacology curricula for the academic year 2000/2001 have been collected from in... more The content of Pharmacology curricula for the academic year 2000/2001 have been collected from individual Medical Schools in Croatia and analysed by using method developed by a Committee of essential knowledge base in Pharmacology, Association for Medical School Pharmacology, USA (URL adress: http: farmr4.uth.tmc.edu/druglist/, network distribution by G.C. Rosenfeld, Department of Pharmacology, University of Texas Medical School, Huston, Texas, USA, 1993). Medical Pharmacology curricula in Croatia are recorded along two dimensions: 1) number of subjects covered and 2) time allocated to individual subject as a fraction (%) of total teaching hours. The "average" Pharmacology curriculum in Croatian Medical Schools (as a mean % total teaching hours of all courses) has been designed and results presented below. N= 4 Mean total teaching hours = 136 (130-148) Subjects Mean % total teaching hours hours range 1) General principles 15.4 (14-27) 2) CNS and peripheral somatic n.s. 11....

The aim of this study was to evaluate effects of hyperbaric oxygen therapy (HBOT) on vascular fun... more The aim of this study was to evaluate effects of hyperbaric oxygen therapy (HBOT) on vascular function in healthy and streptozocin-induced diabetic rats (DM) and to elucidate the mechanisms by which HBOT might protect vascular relaxation. Fifty-five male Sprague Dawley rats were randomized into five groups: (A) rats treated with citric acid buffer, (B) healthy controls, (C) DM+HBOT, (D) DM and (E) healthy controls + HBOT. Vascular responses to cumulative concentration of acetylcholine or SNP in PE-precontracted aortic rings were measured with/without presence of indomethacin, L-NAME or clotrimazol. Plasma antioxidative capacity was measured with FRAP assays and the systemic oxidative stress with TBARS assay. The data were analyzed with two-way ANOVA RM and Bonferroni posttest and considered significant if p<0.05. Vascular relaxation to 10-7M of ACh, in absence of a any inhibitor, was significantly improved (p<0, 001) in healthy + HBOT rats ; furthermore the same group showed e...

Introduction: Red wine is antioxidant and direct, endothelium-dependent vasodilator. Diabetes mel... more Introduction: Red wine is antioxidant and direct, endothelium-dependent vasodilator. Diabetes mellitus (DM) alters vascular responsiveness as a result of oxidative stress and chronic high blood glucose. The present study was designed to examine red wine vasodilatory activity in the rats with streptozotocin-induced type 1 DM. Materials and methods: DM was induced with intaperitoneal injection of streptozotocin (55 mg/kg) to male Sprague-Dawley rats. The DM rats were treated once a week with long-lasting insulin therapy. 14 months after induction of DM, control (C, non- diabetic, 14 months old) and diabetic rats were anesthetized and thoracic aortas excised (N=15 per group). The isolated aortic rings were precontracted with noradrenaline (10-7 M) and randomly exposed to red wine (“Zlatan Plavac” 2008, Plenkovic), acetylcholine or sodium nitroprusside. Vasodilation was measured, dose- response curves analyzed. Results: Red wine induced vasodilation in control and diabetic aorta. DM did...

Phenolic compounds are naturally occurring substances in fruit, vegetables, nuts, seeds, flowers,... more Phenolic compounds are naturally occurring substances in fruit, vegetables, nuts, seeds, flowers, and some herb beverages, and are integral part of human diet. Several studies have indicated that the antioxidant activities of some fruits and vegetables were highly correlated to their total phenolic contents. Hertog et al. [1] found an epidemiological link between the intake of polyphenols and the decreased risk of cardiovascular disease in human. Other studies have shown evidence of in vivo antioxidant effects after ingestion of polyphenol-rich beverages, such as tea and red wine [2]. It seems, according to the present state of knowledge, that positive health effect of these beverages can be connected with the presence of polyphenols catechins. The aim of this study was to investigate the antioxidant effectiveness of pure polyphenolic compound (+)-catechin (flavan-3-ol) and red and white wines using ferric reducing/antioxidant power (FRAP) assay, a rapid direct test of antioxidant c...

Rural and Remote Health, 2013

Can genetics aggravate the health of isolated and remote populations? The case of gout, hyperuric... more Can genetics aggravate the health of isolated and remote populations? The case of gout, hyperuricaemia and osteoarthritis in Dalmatia

Journal of Endocrinological Investigation, 2017

Respiration, 1999

Background: Abnormalities in connective tissue and spirometric disorders have previously been fou... more Background: Abnormalities in connective tissue and spirometric disorders have previously been found in women with genital descensus. Objective: To evaluate the association of descensus and respiratory function. Methods: The blood gases and sex hormones were measured in 130 women scheduled for surgical correction of descensus and 60 matched women without descensus. All subjects were nonsmokers and without past or present cardiorespiratory disease. Results: Women with descensus had a lower pH (7.39 ± 0.04 vs. 7.41 ± 0.04, p = 0.01), lower arterial tensions of oxygen (12.7 ± 12. vs. 14.1 ± 0.9 kPa, p = 0.003) and carbon dioxide (5.1 ± 0.4 vs. 5.3 ± 0.3 kPa) but a higher hemoglobin concentration (141 ± 11 vs. 132 ± 9 g/l) and a higher serum progesterone in the follicular phase of the cycle (3.1 ± 4 vs. 1.5 ± 1 ng/ml, p = 0.03). In 39 (30%) women with descensus, the arterial carbon dioxide tension was below 4.9 kPa. All subjects ventilated more in the luteal compared to the follicular ph...

Nature Genetics, 2012

This paper has been peer-reviewed but dos not include the final publisher proof-corrections or jo... more This paper has been peer-reviewed but dos not include the final publisher proof-corrections or journal pagination.

Journal of the American Society of Nephrology, 2013

Nature genetics, Jan 13, 2012

Recent genome-wide association studies have described many loci implicated in type 2 diabetes (T2... more Recent genome-wide association studies have described many loci implicated in type 2 diabetes (T2D) pathophysiology and β-cell dysfunction but have contributed little to the understanding of the genetic basis of insulin resistance. We hypothesized that genes implicated in insulin resistance pathways might be uncovered by accounting for differences in body mass index (BMI) and potential interactions between BMI and genetic variants. We applied a joint meta-analysis approach to test associations with fasting insulin and glucose on a genome-wide scale. We present six previously unknown loci associated with fasting insulin at P…

Human Molecular Genetics, 2012

In conducting genome-wide association studies (GWAS), analytical approaches leveraging biological... more In conducting genome-wide association studies (GWAS), analytical approaches leveraging biological information may further understanding of the pathophysiology of clinical traits. To discover novel associations with estimated glomerular filtration rate (eGFR), a measure of kidney function, we developed a strategy for integrating prior biological knowledge into the existing GWAS data for eGFR from the CKDGen Consortium. Our strategy focuses on single nucleotide polymorphism (SNPs) in genes that are connected by functional evidence, determined by literature mining and gene ontology (GO) hierarchies, to genes near previously validated eGFR associations. It then requires association thresholds consistent with multiple testing, and finally evaluates novel candidates by independent replication. Among the samples of European ancestry, we identified a genome-wide significant SNP in FBXL20 (P 5 5.6 3 10 29) in meta-analysis of all available data, and additional SNPs at the INHBC, LRP2, PLEKHA1, SLC3A2 and SLC7A6 genes meeting multiple-testing corrected significance for replication and overall P-values of 4.5 3 10 24-2.2 3 10 27. Neither the novel PLEKHA1 nor FBXL20 associations, both further supported by association with eGFR among African Americans and with transcript abundance, would have been implicated by eGFR candidate gene approaches. LRP2, encoding the megalin receptor, was identified through connection with the previously known eGFR gene DAB2 and extends understanding of the megalin system in kidney function. These findings highlight integration of existing genome-wide association data with independent biological knowledge to uncover novel candidate eGFR associations, including candidates lacking known connections to kidney-specific pathways. The strategy may also be applicable to other clinical phenotypes, although more testing will be needed to assess its potential for discovery in general.

5th Croatian congress of …, 2007

Pregled bibliografske jedinice broj: 306887. Zbornik radova. Autori: Musić, Ivana; Modun, Darko; ... more Pregled bibliografske jedinice broj: 306887. Zbornik radova. Autori: Musić, Ivana; Modun, Darko; Vuković, Jonatan; Brizić, Ivica; Katalinić, Višnja; Kozina, Bernard; Boban, Mladen. Naslov: Antioxidative and vasodilatatory effects of phenolic acids from wine. ...

5th Croatian congress …, 2007

... Bibliographic record number: 306881. Journal. Authors: Brizić, Ivica; Lukšić, Boris; Modun, D... more ... Bibliographic record number: 306881. Journal. Authors: Brizić, Ivica; Lukšić, Boris; Modun, Darko; Vuković, Jonatan; Musić, Ivana; Boban, Mladen. Title: Cardioprotective effects of 2, 3-butanendione monoxime against the vipera ...

2nd Congress of the …, 2006

... Bibliographic record number: 277773. Journal. Authors: Modun, Darko; Musić, Ivana; Vuković, J... more ... Bibliographic record number: 277773. Journal. Authors: Modun, Darko; Musić, Ivana; Vuković, Jonatan; Brizić, Ivica; Katalinić, Višnja; Boban, Mladen. Title: Mechanisms Of Red Wine Antioxidative Effects In Humans. Source: Book ...

Journal of Clinical Medicine

COVID-19-associated vascular disease complications are primarily associated with endothelial dysf... more COVID-19-associated vascular disease complications are primarily associated with endothelial dysfunction; however, the consequences of disease on vascular structure and function, particularly in the long term (>7 weeks post-infection), remain unexplored. Individual pre- and post-infection changes in arterial stiffness as well as central and systemic hemodynamic parameters were measured in patients diagnosed with mild COVID-19. As part of in-laboratory observational studies, baseline measurements were taken up to two years before, whereas the post-infection measurements were made 2–3 months after the onset of COVID-19. We used the same measurement protocol throughout the study as well as linear and mixed-effects regression models to analyze the data. Patients (N = 32) were predominantly healthy and young (mean age ± SD: 36.6 ± 12.6). We found that various parameters of arterial stiffness and central hemodynamics—cfPWV, AIx@HR75, and cDBP as well as DBP and MAP—responded to a mild ...

Frontiers in Cardiovascular Medicine

BackgroundLarge longitudinal studies with repeated pulse wave velocity (PWV) measurements, a dire... more BackgroundLarge longitudinal studies with repeated pulse wave velocity (PWV) measurements, a direct measure of arterial stiffness, are required to realize the full potential of arterial stiffness in clinical practice. To facilitate such studies it is important to increase the power of a study by reducing within-subject variability of PWV, and to ease the use of a PWV device in clinical settings by minimizing PWV measurement difficulties.MethodsWe systematically investigated experimental setting and meteorological conditions, as well as physiological factors and participant characteristics, to determine whether and to what extent they affected: between- and within-subjects variability of PWV recordings, and measurement difficulties of a particular device. We conducted a 2-week longitudinal block-randomized cross-over study with two blinded observers and two commonly used devices: applanation tonometry SphygmoCor CvMS and oscillometric Arteriograph to assess carotid-femoral (cfPWV) or...

Analizom 128 povijesti bolesti radnika tvornice "Jugovinil" koji su u desetogodisnjem r... more Analizom 128 povijesti bolesti radnika tvornice "Jugovinil" koji su u desetogodisnjem razdoblju lijeceni na Klinici za unutarnje bolesti KBC "Firule" - Split, utvrđeno je da je njih 32 (25%) lijeceno zbog hepatopatije, a po svojoj je prilici posljedica hepatotokickog ucinka vinilklorid monomera (VCM), kome su ovi radnici nerijetko eksponirani u visokoj koncentraciji. Prikazani su histoloski nalazi biopsije koji se povezuje s VCM ostecenjem: toksicni hepatitis fibroza, ciroza, angiosarkom i hemangiopericitom. Posebna je pažnja posvecena ovim posljednjim entitetima: angiosarkomu zbog vrlo nepovoljne prognoze, a hemangiopericitomu jos zbog toga sto je njegova povezanost s ekspozicijom VCM-u sada jos prvi put uocena.

We tested the effects of some phenolic acid derivatives of red wine on the contraction of rat aor... more We tested the effects of some phenolic acid derivatives of red wine on the contraction of rat aortic and porcine coronary rings. Wine as a mixture of different drugs relaxes all blood vessels, and most of the substances used in our study relax the isolated vessels, too.

The content of Pharmacology curricula for the academic year 2000/2001 have been collected from in... more The content of Pharmacology curricula for the academic year 2000/2001 have been collected from individual Medical Schools in Croatia and analysed by using method developed by a Committee of essential knowledge base in Pharmacology, Association for Medical School Pharmacology, USA (URL adress: http: farmr4.uth.tmc.edu/druglist/, network distribution by G.C. Rosenfeld, Department of Pharmacology, University of Texas Medical School, Huston, Texas, USA, 1993). Medical Pharmacology curricula in Croatia are recorded along two dimensions: 1) number of subjects covered and 2) time allocated to individual subject as a fraction (%) of total teaching hours. The "average" Pharmacology curriculum in Croatian Medical Schools (as a mean % total teaching hours of all courses) has been designed and results presented below. N= 4 Mean total teaching hours = 136 (130-148) Subjects Mean % total teaching hours hours range 1) General principles 15.4 (14-27) 2) CNS and peripheral somatic n.s. 11....

The aim of this study was to evaluate effects of hyperbaric oxygen therapy (HBOT) on vascular fun... more The aim of this study was to evaluate effects of hyperbaric oxygen therapy (HBOT) on vascular function in healthy and streptozocin-induced diabetic rats (DM) and to elucidate the mechanisms by which HBOT might protect vascular relaxation. Fifty-five male Sprague Dawley rats were randomized into five groups: (A) rats treated with citric acid buffer, (B) healthy controls, (C) DM+HBOT, (D) DM and (E) healthy controls + HBOT. Vascular responses to cumulative concentration of acetylcholine or SNP in PE-precontracted aortic rings were measured with/without presence of indomethacin, L-NAME or clotrimazol. Plasma antioxidative capacity was measured with FRAP assays and the systemic oxidative stress with TBARS assay. The data were analyzed with two-way ANOVA RM and Bonferroni posttest and considered significant if p<0.05. Vascular relaxation to 10-7M of ACh, in absence of a any inhibitor, was significantly improved (p<0, 001) in healthy + HBOT rats ; furthermore the same group showed e...

Introduction: Red wine is antioxidant and direct, endothelium-dependent vasodilator. Diabetes mel... more Introduction: Red wine is antioxidant and direct, endothelium-dependent vasodilator. Diabetes mellitus (DM) alters vascular responsiveness as a result of oxidative stress and chronic high blood glucose. The present study was designed to examine red wine vasodilatory activity in the rats with streptozotocin-induced type 1 DM. Materials and methods: DM was induced with intaperitoneal injection of streptozotocin (55 mg/kg) to male Sprague-Dawley rats. The DM rats were treated once a week with long-lasting insulin therapy. 14 months after induction of DM, control (C, non- diabetic, 14 months old) and diabetic rats were anesthetized and thoracic aortas excised (N=15 per group). The isolated aortic rings were precontracted with noradrenaline (10-7 M) and randomly exposed to red wine (“Zlatan Plavac” 2008, Plenkovic), acetylcholine or sodium nitroprusside. Vasodilation was measured, dose- response curves analyzed. Results: Red wine induced vasodilation in control and diabetic aorta. DM did...

Phenolic compounds are naturally occurring substances in fruit, vegetables, nuts, seeds, flowers,... more Phenolic compounds are naturally occurring substances in fruit, vegetables, nuts, seeds, flowers, and some herb beverages, and are integral part of human diet. Several studies have indicated that the antioxidant activities of some fruits and vegetables were highly correlated to their total phenolic contents. Hertog et al. [1] found an epidemiological link between the intake of polyphenols and the decreased risk of cardiovascular disease in human. Other studies have shown evidence of in vivo antioxidant effects after ingestion of polyphenol-rich beverages, such as tea and red wine [2]. It seems, according to the present state of knowledge, that positive health effect of these beverages can be connected with the presence of polyphenols catechins. The aim of this study was to investigate the antioxidant effectiveness of pure polyphenolic compound (+)-catechin (flavan-3-ol) and red and white wines using ferric reducing/antioxidant power (FRAP) assay, a rapid direct test of antioxidant c...

Rural and Remote Health, 2013

Can genetics aggravate the health of isolated and remote populations? The case of gout, hyperuric... more Can genetics aggravate the health of isolated and remote populations? The case of gout, hyperuricaemia and osteoarthritis in Dalmatia

Journal of Endocrinological Investigation, 2017

Respiration, 1999

Background: Abnormalities in connective tissue and spirometric disorders have previously been fou... more Background: Abnormalities in connective tissue and spirometric disorders have previously been found in women with genital descensus. Objective: To evaluate the association of descensus and respiratory function. Methods: The blood gases and sex hormones were measured in 130 women scheduled for surgical correction of descensus and 60 matched women without descensus. All subjects were nonsmokers and without past or present cardiorespiratory disease. Results: Women with descensus had a lower pH (7.39 ± 0.04 vs. 7.41 ± 0.04, p = 0.01), lower arterial tensions of oxygen (12.7 ± 12. vs. 14.1 ± 0.9 kPa, p = 0.003) and carbon dioxide (5.1 ± 0.4 vs. 5.3 ± 0.3 kPa) but a higher hemoglobin concentration (141 ± 11 vs. 132 ± 9 g/l) and a higher serum progesterone in the follicular phase of the cycle (3.1 ± 4 vs. 1.5 ± 1 ng/ml, p = 0.03). In 39 (30%) women with descensus, the arterial carbon dioxide tension was below 4.9 kPa. All subjects ventilated more in the luteal compared to the follicular ph...

Nature Genetics, 2012

This paper has been peer-reviewed but dos not include the final publisher proof-corrections or jo... more This paper has been peer-reviewed but dos not include the final publisher proof-corrections or journal pagination.

Journal of the American Society of Nephrology, 2013

Nature genetics, Jan 13, 2012

Recent genome-wide association studies have described many loci implicated in type 2 diabetes (T2... more Recent genome-wide association studies have described many loci implicated in type 2 diabetes (T2D) pathophysiology and β-cell dysfunction but have contributed little to the understanding of the genetic basis of insulin resistance. We hypothesized that genes implicated in insulin resistance pathways might be uncovered by accounting for differences in body mass index (BMI) and potential interactions between BMI and genetic variants. We applied a joint meta-analysis approach to test associations with fasting insulin and glucose on a genome-wide scale. We present six previously unknown loci associated with fasting insulin at P…

Human Molecular Genetics, 2012

In conducting genome-wide association studies (GWAS), analytical approaches leveraging biological... more In conducting genome-wide association studies (GWAS), analytical approaches leveraging biological information may further understanding of the pathophysiology of clinical traits. To discover novel associations with estimated glomerular filtration rate (eGFR), a measure of kidney function, we developed a strategy for integrating prior biological knowledge into the existing GWAS data for eGFR from the CKDGen Consortium. Our strategy focuses on single nucleotide polymorphism (SNPs) in genes that are connected by functional evidence, determined by literature mining and gene ontology (GO) hierarchies, to genes near previously validated eGFR associations. It then requires association thresholds consistent with multiple testing, and finally evaluates novel candidates by independent replication. Among the samples of European ancestry, we identified a genome-wide significant SNP in FBXL20 (P 5 5.6 3 10 29) in meta-analysis of all available data, and additional SNPs at the INHBC, LRP2, PLEKHA1, SLC3A2 and SLC7A6 genes meeting multiple-testing corrected significance for replication and overall P-values of 4.5 3 10 24-2.2 3 10 27. Neither the novel PLEKHA1 nor FBXL20 associations, both further supported by association with eGFR among African Americans and with transcript abundance, would have been implicated by eGFR candidate gene approaches. LRP2, encoding the megalin receptor, was identified through connection with the previously known eGFR gene DAB2 and extends understanding of the megalin system in kidney function. These findings highlight integration of existing genome-wide association data with independent biological knowledge to uncover novel candidate eGFR associations, including candidates lacking known connections to kidney-specific pathways. The strategy may also be applicable to other clinical phenotypes, although more testing will be needed to assess its potential for discovery in general.

5th Croatian congress of …, 2007

Pregled bibliografske jedinice broj: 306887. Zbornik radova. Autori: Musić, Ivana; Modun, Darko; ... more Pregled bibliografske jedinice broj: 306887. Zbornik radova. Autori: Musić, Ivana; Modun, Darko; Vuković, Jonatan; Brizić, Ivica; Katalinić, Višnja; Kozina, Bernard; Boban, Mladen. Naslov: Antioxidative and vasodilatatory effects of phenolic acids from wine. ...

5th Croatian congress …, 2007

... Bibliographic record number: 306881. Journal. Authors: Brizić, Ivica; Lukšić, Boris; Modun, D... more ... Bibliographic record number: 306881. Journal. Authors: Brizić, Ivica; Lukšić, Boris; Modun, Darko; Vuković, Jonatan; Musić, Ivana; Boban, Mladen. Title: Cardioprotective effects of 2, 3-butanendione monoxime against the vipera ...

2nd Congress of the …, 2006

... Bibliographic record number: 277773. Journal. Authors: Modun, Darko; Musić, Ivana; Vuković, J... more ... Bibliographic record number: 277773. Journal. Authors: Modun, Darko; Musić, Ivana; Vuković, Jonatan; Brizić, Ivica; Katalinić, Višnja; Boban, Mladen. Title: Mechanisms Of Red Wine Antioxidative Effects In Humans. Source: Book ...