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Papers by Mohamad Gadelmoula

Research paper thumbnail of MP-16.09: Galectin-3 is overexpressed in renal cell carcinoma and it regulates immune suppression through the induction of apoptosis of cytotoxic T cells

Urology, 2007

Introduction: To investigate the role of epigenetic gene silencing in the pathogenesis of renal c... more Introduction: To investigate the role of epigenetic gene silencing in the pathogenesis of renal cell carcinoma (RCC), we determined their methylation profile and analyzed its correlation with clinical outcomes. Methods: Genomic DNA and RNA were extracted from RCC tissue samples with their corresponding normal kidney samples. Promoter methylation at RASSF1A, DUTT1, SLIT 2, HGF activator inhibitor type 2 (HAI-2), was analyzed by methylation-specific PCR and the results were verified by DNA sequencing. Results: RASSF1A promoter methylation was detected in 23% (32 of 138) of primary clear cell RCC tumors and 44 % (12 of 27) of papillary RCC tumours. DUTT1 promoter methylation was detected in 18 % (8 of 44) of clear cell RCC tumours. SLIT 2 promoter methylation was detected in 25% (12 of 48) of clear cell RCC tumours. HAI-2 promoter methylation was detected in 30 % (19 of 64) of clear cell RCC and 40 % (15 of 38) of papillary RCC. Kaplan-Meier analysis revealed that only the hypermethylation of DUTT1 correlated with disease specific survival. Conclusion: These results indicate that the promoter methylation of these candidate tumor suppressor genes may play an important role in tumour genesis in RCC and some of them could predict the prognosis of RCC patients. MP-16.10 Nitrogen-containing bisphosphonates inhibit the growth of renal cell carcinoma cells

Research paper thumbnail of MP-06.04: Hem-o-lok polymer ligating clip for vascular control of the renal pedicle: points of controversy

Research paper thumbnail of POS-02.15: The expression of claudin-7 in transitional cell carcinoma of the urinary bladder: a quantitative reverse transcriptase-polymerase chain reaction analysis

Urology, 2007

The aim of the present study was to evaluate the status of HER2 protein expression in patients wi... more The aim of the present study was to evaluate the status of HER2 protein expression in patients with muscle-invasive urothelial carcinomas of bladder treated with radical cystectomy and correlate it with clinical outcome. Material and Methods: We retrospectively analyzed the data of 90 patients who underwent cystectomy for invasive transitional cell carcinoma of urinary bladder. Immunohistochemical analysis for Her2/neu was done on paraffin-fixed tissues with CB11 antibodies (BioGenex, San Ramon, CA). Sections with grade 2 and 3 staining were considered positive for Her2/neu. Results: Over a median follow-up of 46 months (24-96 months) 46 patients are living without disease recurrence and 6 with recurrent disease either at local site or with distant metastasis. The remaining 38 patients have died. The median overall survival (OAS) was 50 months and median disease free survival (DFS) was 40 months. The Her2/neu status was significantly related to the tumor stage (pϭ0.02), lymphnode involvement (77% in Nϩ vs 23% in N0; pϭ0.01) and the grade of the disease (32% of grade 2 vs 71% of grade 3; pϭ0.042). In addition to tumor stage and lymphnode status Kaplan-Meier curves showed a significantly worse disease related survival (pϭ0.001) in patients with Her-2 overexpressing tumors compared to those without overexpression.On multivariate analysis her2/neu overexpression was found to be an independent predictive factor of disease recurrence. Conclusion: These results suggest that Her2 expression might provide additional prognostic information in patients with muscle-invasive bladder cancer. Future studies on Her2 expression with chemosensitivity and the efficacy of Her2 targeted therapies in urothelial carcinomas are warranted.

Research paper thumbnail of Combined Mathieu and Snodgrass urethroplasty for hypospadias repair: A prospective randomized study

International Journal of Urology, 2010

Objectives: To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal h... more Objectives: To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias repair and to compare them with the two techniques separately. Methods: Between January 2006 and February 2009, patients with distal hypospadias were prospectively randomized to undergo one of the three following urethroplasty techniques: Mathieu urethroplasty, Snodgrass urethroplasty or a combination of the two. Operative time, intraoperative, early and late postoperative complications were reported for each procedure. Results: 101 patients were included in this study. The Mathieu technique was used for 30 patients, Snodgrass repair was carried out in 37 patients and 34 patients underwent the combined technique. Operative time ranged from 43 to 120 min. Eight patients developed urethrocutaneous fistulae. Meatal stenosis was encountered in five cases. Thirty-seven patients had rounded meatus, while a slit-like urethral opening was found in 64 cases. Conclusions: In our hands, the combined Mathieu and Snodgrass urethroplasty technique provided a better cosmetic outcome than the Mathieu technique with no incidence of meatal stenosis as seen with the Snodgrass technique.

Research paper thumbnail of Deferred endoscopic urethral realignment: Role in management of traumatic posterior urethral disruption

African Journal of Urology, 2014

The aim of our study is to assess the value of deferred endoscopic urethral realignment after tra... more The aim of our study is to assess the value of deferred endoscopic urethral realignment after traumatic posterior urethral disruption. Patients and methods: Between June 2001 and August 2011, we evaluated 28 patients who presented 3-6 weeks (mean 27 ± 6 days) after experiencing traumatic posterior urethral disruptions and pelvic fractures; immediate and early realignment were overdue in these cases. Patient variables included mode of presentation, mechanism of trauma, type of pelvic fracture, and Abbreviated Injury Scale (AIS). Under fluoroscopic guidance, a guidewire was passed into the injured urethral segment from the distal to proximal injured ends using a long Chiba needle, and realignment was performed using endoscopic urethrotomy. The follow-up period ranged from 18 to 98 months (mean 43 ± 22.5 months).

Research paper thumbnail of Laparoscopy for impalpable testis: classification-based management

Surgical Endoscopy and Other Interventional Techniques, 2007

Background The undescended testis represents one of the most common disorders of childhood. The ... more Background The undescended testis represents one of the most common disorders of childhood. The authors evaluated the safety and efficacy of laparoscopy for the abdominal testis and present a classification of the laparoscopic diagnostic findings to facilitate decision making. Methods Between 2000 and 2005, 95 patients (22 bilateral and 73 unilateral testes, for a total of 117 impalpable testes) with a mean

Research paper thumbnail of 1328: Laparoscopic Ureterolithotomy: Bi-Center Experience of 74 Cases

Research paper thumbnail of 586: Laparoscopy for Impalpable Testis and Intersex: Report of 103 Cases

Research paper thumbnail of VP-037 Phalloplasty with Forearm Free Flap : Single Center Experience with 6 Cases of Male Pseudo-hermaphrodite

The Japanese Journal of Urology

Research paper thumbnail of OP-260 Laparoscopic Ureterolithotomy

The Japanese Journal of Urology, 2007

Research paper thumbnail of Loss of the Tight Junction Protein CLAUDIN-7 Correlates with Carcinogenesis and Progression of the Transitional Cell Carcinoma of the Urinary Bladder

The Journal of Urology, 2009

Research paper thumbnail of Laparoscopic Nephropexy

Journal of Laparoendoscopic & Advanced Surgical Techniques, 2008

We evaluated the safety and efficacy of laparoscopic nephropexy for symptomatic nephrotosis. Betw... more We evaluated the safety and efficacy of laparoscopic nephropexy for symptomatic nephrotosis. Between May 2000 and December 2005, 6 women (mean age, 43.1 years) underwent laparoscopic nephropexy of 8 kidneys (6 right, 2 left). Mean body mass index was 19.6 kg/m(2). Two patients displayed ureteropelvic junction obstruction (UPJO). Preoperative evaluation was performed by intravenous urography (IVU) and isotope renography in both the supine and erect positions. Four patients (6 renal units) underwent retroperitoneoscopic nephropexy, with a dismembered pyeloplasty in 1 patient (1 renal unit). The transperitoneal approach was performed in 2 patients, the first case and another with UPJO due to an aberrant vessel. The kidney was mobilized, then fixed to the back muscles with nonabsorbable sutures. Mean operative time was 139.4 minutes (range, 95-200), and mean blood loss was 30 mL (range, 10-60). No perioperative complications were encountered. Mean hospital stay was 6.4 days. Postoperative IVU and isotope renography correctly confirmed a fixed kidney and no obstruction in the UPJO cases. All patients reported a symptomatic improvement during follow-up (range, 11-81 months). As pain is the presenting symptom in these cases, a laparoscopic approach seems to represent the gold standard for treatment owing to low morbidity and good cosmetic results.

Research paper thumbnail of Laparoscopic ureterolithotomy: Our experience with 74 cases

International Journal of Urology, 2008

We retrospectively evaluated our experience with a relatively uncommon procedure, the laparoscopi... more We retrospectively evaluated our experience with a relatively uncommon procedure, the laparoscopic ureterolithotomy, for the treatment of ureteral stones. Between April 2002 and October 2006, a total of 74 patients (56 males, 18 females) with upper (54 cases), middle (18 cases) and lower (two cases) ureteral stones underwent laparoscopic ureterolithomy. The mean age was 39.4 years (range, 19-74). The stones were in the right side in 44 cases (59.5%) and in the left side in 30 (40.5%) cases. The mean stone size was 1.8 cm (range 1.5-2.8). The procedure was retroperitoneal in 66 cases (89.2%) and transperitoneal in eight (10.8%) cases. Laparoscopic guided flexible ureterorenoscopic extraction of kidney stone was carried out in one case as an adjuvant procedure. The ureter was stented and not sutured in 64 cases (86.5%). The procedure was successfully completed in 94.6% of cases and an open conversion was carried out in four (5.4%) patients. The mean operative time was 58.7 min, and the mean blood loss was 90.6 mL. No major complications were encountered. Prolonged urinary leakage occurred in one patient. The mean hospital stay was 6.4 days. One patient developed ureteral stricture during follow up and was treated by endoscopic dilatation and stenting. In our experience laparoscopic ureterolithotomy represents a safe and effective treatment option for ureteral stones either as primary for large impacted stones or as a salvage procedure after failed shock wave lithotripsy or ureteroscopy. This procedure fulfills the advantages of minimal blood loss and analgesia requirements, good cosmetic appearance, short hospital stay and convalescence period.

Research paper thumbnail of Laparoscopy and intersex: report of 5 cases of male pseudohermaphroditism

The Journal of …, 2008

Laparoscopy was recently introduced as a method of evaluation and treatment of intersex patients ... more Laparoscopy was recently introduced as a method of evaluation and treatment of intersex patients pre-sented by impalpable testes. Besides its advantage as a minimally invasive approach, its better cosmetic result is an important factor for those patients with psychological and/or ...

Research paper thumbnail of Combined Mathieu and Snodgrass urethroplasty for hypospadias repair: A prospective randomized study

International Journal of Urology, 2010

To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias r... more To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias repair and to compare them with the two techniques separately. Methods: Between January 2006 and February 2009, patients with distal hypospadias were prospectively randomized to undergo one of the three following urethroplasty techniques: Mathieu urethroplasty, Snodgrass urethroplasty or a combination of the two. Operative time, intraoperative, early and late postoperative complications were reported for each procedure. Results: 101 patients were included in this study. The Mathieu technique was used for 30 patients, Snodgrass repair was carried out in 37 patients and 34 patients underwent the combined technique. Operative time ranged from 43 to 120 min. Eight patients developed urethrocutaneous fistulae. Meatal stenosis was encountered in five cases. Thirty-seven patients had rounded meatus, while a slit-like urethral opening was found in 64 cases. Conclusions: In our hands, the combined Mathieu and Snodgrass urethroplasty technique provided a better cosmetic outcome than the Mathieu technique with no incidence of meatal stenosis as seen with the Snodgrass technique.

Research paper thumbnail of MP-16.09: Galectin-3 is overexpressed in renal cell carcinoma and it regulates immune suppression through the induction of apoptosis of cytotoxic T cells

Urology, 2007

Introduction: To investigate the role of epigenetic gene silencing in the pathogenesis of renal c... more Introduction: To investigate the role of epigenetic gene silencing in the pathogenesis of renal cell carcinoma (RCC), we determined their methylation profile and analyzed its correlation with clinical outcomes. Methods: Genomic DNA and RNA were extracted from RCC tissue samples with their corresponding normal kidney samples. Promoter methylation at RASSF1A, DUTT1, SLIT 2, HGF activator inhibitor type 2 (HAI-2), was analyzed by methylation-specific PCR and the results were verified by DNA sequencing. Results: RASSF1A promoter methylation was detected in 23% (32 of 138) of primary clear cell RCC tumors and 44 % (12 of 27) of papillary RCC tumours. DUTT1 promoter methylation was detected in 18 % (8 of 44) of clear cell RCC tumours. SLIT 2 promoter methylation was detected in 25% (12 of 48) of clear cell RCC tumours. HAI-2 promoter methylation was detected in 30 % (19 of 64) of clear cell RCC and 40 % (15 of 38) of papillary RCC. Kaplan-Meier analysis revealed that only the hypermethylation of DUTT1 correlated with disease specific survival. Conclusion: These results indicate that the promoter methylation of these candidate tumor suppressor genes may play an important role in tumour genesis in RCC and some of them could predict the prognosis of RCC patients. MP-16.10 Nitrogen-containing bisphosphonates inhibit the growth of renal cell carcinoma cells

Research paper thumbnail of MP-06.04: Hem-o-lok polymer ligating clip for vascular control of the renal pedicle: points of controversy

Research paper thumbnail of POS-02.15: The expression of claudin-7 in transitional cell carcinoma of the urinary bladder: a quantitative reverse transcriptase-polymerase chain reaction analysis

Urology, 2007

The aim of the present study was to evaluate the status of HER2 protein expression in patients wi... more The aim of the present study was to evaluate the status of HER2 protein expression in patients with muscle-invasive urothelial carcinomas of bladder treated with radical cystectomy and correlate it with clinical outcome. Material and Methods: We retrospectively analyzed the data of 90 patients who underwent cystectomy for invasive transitional cell carcinoma of urinary bladder. Immunohistochemical analysis for Her2/neu was done on paraffin-fixed tissues with CB11 antibodies (BioGenex, San Ramon, CA). Sections with grade 2 and 3 staining were considered positive for Her2/neu. Results: Over a median follow-up of 46 months (24-96 months) 46 patients are living without disease recurrence and 6 with recurrent disease either at local site or with distant metastasis. The remaining 38 patients have died. The median overall survival (OAS) was 50 months and median disease free survival (DFS) was 40 months. The Her2/neu status was significantly related to the tumor stage (pϭ0.02), lymphnode involvement (77% in Nϩ vs 23% in N0; pϭ0.01) and the grade of the disease (32% of grade 2 vs 71% of grade 3; pϭ0.042). In addition to tumor stage and lymphnode status Kaplan-Meier curves showed a significantly worse disease related survival (pϭ0.001) in patients with Her-2 overexpressing tumors compared to those without overexpression.On multivariate analysis her2/neu overexpression was found to be an independent predictive factor of disease recurrence. Conclusion: These results suggest that Her2 expression might provide additional prognostic information in patients with muscle-invasive bladder cancer. Future studies on Her2 expression with chemosensitivity and the efficacy of Her2 targeted therapies in urothelial carcinomas are warranted.

Research paper thumbnail of Combined Mathieu and Snodgrass urethroplasty for hypospadias repair: A prospective randomized study

International Journal of Urology, 2010

Objectives: To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal h... more Objectives: To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias repair and to compare them with the two techniques separately. Methods: Between January 2006 and February 2009, patients with distal hypospadias were prospectively randomized to undergo one of the three following urethroplasty techniques: Mathieu urethroplasty, Snodgrass urethroplasty or a combination of the two. Operative time, intraoperative, early and late postoperative complications were reported for each procedure. Results: 101 patients were included in this study. The Mathieu technique was used for 30 patients, Snodgrass repair was carried out in 37 patients and 34 patients underwent the combined technique. Operative time ranged from 43 to 120 min. Eight patients developed urethrocutaneous fistulae. Meatal stenosis was encountered in five cases. Thirty-seven patients had rounded meatus, while a slit-like urethral opening was found in 64 cases. Conclusions: In our hands, the combined Mathieu and Snodgrass urethroplasty technique provided a better cosmetic outcome than the Mathieu technique with no incidence of meatal stenosis as seen with the Snodgrass technique.

Research paper thumbnail of Deferred endoscopic urethral realignment: Role in management of traumatic posterior urethral disruption

African Journal of Urology, 2014

The aim of our study is to assess the value of deferred endoscopic urethral realignment after tra... more The aim of our study is to assess the value of deferred endoscopic urethral realignment after traumatic posterior urethral disruption. Patients and methods: Between June 2001 and August 2011, we evaluated 28 patients who presented 3-6 weeks (mean 27 ± 6 days) after experiencing traumatic posterior urethral disruptions and pelvic fractures; immediate and early realignment were overdue in these cases. Patient variables included mode of presentation, mechanism of trauma, type of pelvic fracture, and Abbreviated Injury Scale (AIS). Under fluoroscopic guidance, a guidewire was passed into the injured urethral segment from the distal to proximal injured ends using a long Chiba needle, and realignment was performed using endoscopic urethrotomy. The follow-up period ranged from 18 to 98 months (mean 43 ± 22.5 months).

Research paper thumbnail of Laparoscopy for impalpable testis: classification-based management

Surgical Endoscopy and Other Interventional Techniques, 2007

Background The undescended testis represents one of the most common disorders of childhood. The ... more Background The undescended testis represents one of the most common disorders of childhood. The authors evaluated the safety and efficacy of laparoscopy for the abdominal testis and present a classification of the laparoscopic diagnostic findings to facilitate decision making. Methods Between 2000 and 2005, 95 patients (22 bilateral and 73 unilateral testes, for a total of 117 impalpable testes) with a mean

Research paper thumbnail of 1328: Laparoscopic Ureterolithotomy: Bi-Center Experience of 74 Cases

Research paper thumbnail of 586: Laparoscopy for Impalpable Testis and Intersex: Report of 103 Cases

Research paper thumbnail of VP-037 Phalloplasty with Forearm Free Flap : Single Center Experience with 6 Cases of Male Pseudo-hermaphrodite

The Japanese Journal of Urology

Research paper thumbnail of OP-260 Laparoscopic Ureterolithotomy

The Japanese Journal of Urology, 2007

Research paper thumbnail of Loss of the Tight Junction Protein CLAUDIN-7 Correlates with Carcinogenesis and Progression of the Transitional Cell Carcinoma of the Urinary Bladder

The Journal of Urology, 2009

Research paper thumbnail of Laparoscopic Nephropexy

Journal of Laparoendoscopic & Advanced Surgical Techniques, 2008

We evaluated the safety and efficacy of laparoscopic nephropexy for symptomatic nephrotosis. Betw... more We evaluated the safety and efficacy of laparoscopic nephropexy for symptomatic nephrotosis. Between May 2000 and December 2005, 6 women (mean age, 43.1 years) underwent laparoscopic nephropexy of 8 kidneys (6 right, 2 left). Mean body mass index was 19.6 kg/m(2). Two patients displayed ureteropelvic junction obstruction (UPJO). Preoperative evaluation was performed by intravenous urography (IVU) and isotope renography in both the supine and erect positions. Four patients (6 renal units) underwent retroperitoneoscopic nephropexy, with a dismembered pyeloplasty in 1 patient (1 renal unit). The transperitoneal approach was performed in 2 patients, the first case and another with UPJO due to an aberrant vessel. The kidney was mobilized, then fixed to the back muscles with nonabsorbable sutures. Mean operative time was 139.4 minutes (range, 95-200), and mean blood loss was 30 mL (range, 10-60). No perioperative complications were encountered. Mean hospital stay was 6.4 days. Postoperative IVU and isotope renography correctly confirmed a fixed kidney and no obstruction in the UPJO cases. All patients reported a symptomatic improvement during follow-up (range, 11-81 months). As pain is the presenting symptom in these cases, a laparoscopic approach seems to represent the gold standard for treatment owing to low morbidity and good cosmetic results.

Research paper thumbnail of Laparoscopic ureterolithotomy: Our experience with 74 cases

International Journal of Urology, 2008

We retrospectively evaluated our experience with a relatively uncommon procedure, the laparoscopi... more We retrospectively evaluated our experience with a relatively uncommon procedure, the laparoscopic ureterolithotomy, for the treatment of ureteral stones. Between April 2002 and October 2006, a total of 74 patients (56 males, 18 females) with upper (54 cases), middle (18 cases) and lower (two cases) ureteral stones underwent laparoscopic ureterolithomy. The mean age was 39.4 years (range, 19-74). The stones were in the right side in 44 cases (59.5%) and in the left side in 30 (40.5%) cases. The mean stone size was 1.8 cm (range 1.5-2.8). The procedure was retroperitoneal in 66 cases (89.2%) and transperitoneal in eight (10.8%) cases. Laparoscopic guided flexible ureterorenoscopic extraction of kidney stone was carried out in one case as an adjuvant procedure. The ureter was stented and not sutured in 64 cases (86.5%). The procedure was successfully completed in 94.6% of cases and an open conversion was carried out in four (5.4%) patients. The mean operative time was 58.7 min, and the mean blood loss was 90.6 mL. No major complications were encountered. Prolonged urinary leakage occurred in one patient. The mean hospital stay was 6.4 days. One patient developed ureteral stricture during follow up and was treated by endoscopic dilatation and stenting. In our experience laparoscopic ureterolithotomy represents a safe and effective treatment option for ureteral stones either as primary for large impacted stones or as a salvage procedure after failed shock wave lithotripsy or ureteroscopy. This procedure fulfills the advantages of minimal blood loss and analgesia requirements, good cosmetic appearance, short hospital stay and convalescence period.

Research paper thumbnail of Laparoscopy and intersex: report of 5 cases of male pseudohermaphroditism

The Journal of …, 2008

Laparoscopy was recently introduced as a method of evaluation and treatment of intersex patients ... more Laparoscopy was recently introduced as a method of evaluation and treatment of intersex patients pre-sented by impalpable testes. Besides its advantage as a minimally invasive approach, its better cosmetic result is an important factor for those patients with psychological and/or ...

Research paper thumbnail of Combined Mathieu and Snodgrass urethroplasty for hypospadias repair: A prospective randomized study

International Journal of Urology, 2010

To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias r... more To evaluate the outcomes of combined Mathieu and Snodgrass urethroplasty for distal hypospadias repair and to compare them with the two techniques separately. Methods: Between January 2006 and February 2009, patients with distal hypospadias were prospectively randomized to undergo one of the three following urethroplasty techniques: Mathieu urethroplasty, Snodgrass urethroplasty or a combination of the two. Operative time, intraoperative, early and late postoperative complications were reported for each procedure. Results: 101 patients were included in this study. The Mathieu technique was used for 30 patients, Snodgrass repair was carried out in 37 patients and 34 patients underwent the combined technique. Operative time ranged from 43 to 120 min. Eight patients developed urethrocutaneous fistulae. Meatal stenosis was encountered in five cases. Thirty-seven patients had rounded meatus, while a slit-like urethral opening was found in 64 cases. Conclusions: In our hands, the combined Mathieu and Snodgrass urethroplasty technique provided a better cosmetic outcome than the Mathieu technique with no incidence of meatal stenosis as seen with the Snodgrass technique.