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Research paper thumbnail of Effect of dietary chromium supplementation on productive and reproductive performance of early lactating dairy cows under heat stress

Journal of Animal Physiology and Animal Nutrition, 2010

One hundred and twenty early lactating Holstein dairy cows were assigned to investigate the effec... more One hundred and twenty early lactating Holstein dairy cows were assigned to investigate the effect of dietary chromium (Cr) supplementation (0 or 6 mg Cr/head/day from organic preparation) on the productive and reproductive performance as well as on some blood serum parameters under heat stress (35-40°C). Cows received treatment from 3 weeks pre-partum through 12 weeks post-partum. Chromium supplemented diet had lower body weight loss and improved dry matter intake with consequent reduction in the energy balance deficits during the first period after calving when compared with the control group. Chromium supplementation increased (p < 0.05) milk yield by 6.7%, 12.3% and 16.5% at 4, 8 and 12 weeks post-partum, respectively, whereas milk composition and milk to feed ratio were unaffected (p > 0.05) when compared with the control. Moreover, dietary Cr supplementation had no effect (p > 0.05) on blood serum glucose, calcium and phosphorus concentrations. A reduction (p < 0.05) of non-esterified fatty acids at 1 week pre-partum, 2 and 4 weeks post-partum was also observed. Serum insulin concentration increased whereas cortisol concentration decreased, when compared with the control group, throughout the whole experimental period. Also Cr supplementation showed a trend towards improving reproductive performance as indicated by increased percentage of pregnant cows in the first 28 days of breeding. It could be concluded that dietary Cr supplementation at level of 6 mg/head/day may offer a potential protective management practice to lessen the effect of heat stress in dairy cattle.

Research paper thumbnail of Selection for wingbone breaking strength in cage-reared broilers /

Research paper thumbnail of Neurobehavioural Effect of Zinc Oxide Nanoparticles and its Conventional Form on Adult Male Rats and Their Pups

Alexandria Journal of Veterinary Sciences, 2020

Zinc is involved in many protein structures, which had a role in some neurological functions. The... more Zinc is involved in many protein structures, which had a role in some neurological functions. The present study was designed to investigate the effect of zinc oxide nanoparticles and its conventional one on memory and learning ability of adult male rats and their offspring. Thirty adult male rats were randomly distributed into 5 groups (6 rats, each). First group (control group) was injected with Tween 80 (10%), second and third groups, received two doses of Zinc oxide nanoparticles (ZnONPs), 5 and 10 mg/kg bwt respectively. Fourth and fifth groups, administrated two doses of conventional Zinc oxide (CZnO), 5 and 10 mg/kg bwt, respectively. Rats in all groups were injected intraperitoneally by 1ml/rat for 16 days period, every other day. A series of neurobehavioural tests including open field, elevated plus maze and Morris water maze test were conducted. The obtained results indicated that the ZnONPs lower dose improved locomotor behaviour during open field test and had an anxiolytic effect in the elevated plus maze, but higher doses were not beneficial. It can be concluded that the zinc oxide nanoparticles are better than conventional zinc oxide.

Research paper thumbnail of Bee pollen and propolis as dietary supplements for rabbit: Effect on reproductive performance of does and on immunological response of does and their offspring

Journal of Animal Physiology and Animal Nutrition, 2019

To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 6... more To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 64 nulliparous NZW rabbits does were distributed among eight groups (eight animals/group). One unsupplemented group was the control; the other seven groups were supplemented, respectively, with zinc bacitracin (ZnB) at 100 mg, BP at 150 and 300 mg, Pro at 150 and 300 mg, BP+Pro at 150 and 300 mg of each three times/week, day after day continuously along eight parities. The BP300, Pro300 and BP+Pro150 groups had higher body weight of litter at birth and number of kids born alive. The BP supplementation at 150 mg increased plasma total protein and albumin than the control group. The BP or Pro at 150 mg decreased plasma T3 than the other groups except for BP+Pro150. The ZnB group had significantly greater T3 /T4 ratio compared to BP, Pro and BP+Pro at 150 mg. The BP+Pro150 group had less ALT than the control; BP300 and Pro 300 mg resulted in lower plasma AST than the groups Pro150 with or without BP and the control group. The plasma alkaline phosphatase of BP at 150 or 300 mg and BP+Pro150 was significantly greater than that of the Pro150 group. The BP+Pro300 group had higher WBCs than the other groups. In contrast, the lymphocytes were greater in the Pro and BP+Pro300 groups than in BP, Pro and BP+Pro at 150 mg. The groups supplemented with BP and BP+Pro at 150 and 300 mg had significantly greater SRBCs of doe rabbits and their offspring compared to the control and the ZnB group. The BP at 300 mg increased the serum albumin and α1 -globulin than the control group. The Pro300 group had greater serum α2 -globulin and β-globulin than the control group. The total globulin was significantly greater for the 300 mg propolis-supplemented groups than the control.

Research paper thumbnail of Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits

Journal of Animal Physiology and Animal Nutrition, 2019

Sixty-four nulliparous female rabbits were distributed among eight groups (eight animals/group). ... more Sixty-four nulliparous female rabbits were distributed among eight groups (eight animals/group). Group one was the unsupplemented control group; the other seven groups were supplemented with zinc bacitracin (ZnB) at 100 mg, or bee pollen (BP) and/or propolis (Pro) at 150 and 300 mg in a capsulated form, three times a week, day after day, continuously all over the experimental period. The experiment was run for eight parties; at each parity, 28 kids of each doe group (a total of 224 rabbits) were divided into two subgroups weaned, respectively, at 24 and 30 days of age. Thus, for each parity, there were 16 groups (eight does treatments × two weaning age, 14 rabbits per group). The growing rabbits fed the standard diets without supplements. The growth performance, the carcass traits, the liver and the spleen histology of rabbits were checked up to 90 days of age to find possible carryover effects of the supplements. The supplements had no significant effect on most of the growth performance at 90 days of age, but BP150 and BP+Pro300 increased the growth rate in comparison with ZnB group. The liver weight in the control, BP300 and Pro300 groups was higher than the ZnB one. The spleen weight was higher in the groups ZnB, BP150, Pro300 and BP+Pro300, followed by the control, BP300 and BP+Pro150 and thus Pro150. The heart % in the BP150 and Pro300 groups was higher than ZnB and BP+Pro150 groups. A lymphoid hyperplasia of splenic white pulp was observed in the BP+Pro groups, while propolis alone showed a mild activation of lymphobiosis. The Pro and BP groups showed the same picture of the control group exhibiting a hydropic degeneration of mostly hepatic cells, while the ZnB group exhibited adverse effect on the bile ducts featuring portal periductal inflammatory cells infiltration with epithelial hyperplasia reflecting chronic cholangitis. K E Y W O R D S bee pollen, carryover effects, growing rabbits, propolis How to cite this article: Attia YA, Bovera F, Abd-Elhamid AEE, et al. Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits. J

Research paper thumbnail of Effect of Feeding Graded Levels of Urea on Growing New Zealand White Rabbit Performance

Forty New Zealand white rabbits of about 1340 g live weight were used to evaluate the influence o... more Forty New Zealand white rabbits of about 1340 g live weight were used to evaluate the influence of diet supplemented with urea at different levels on growth performance, blood cellular and biochemical parameters. The rabbits were assigned into four dietary treatments control, 3 urea-supplemented groups (0.5%, 1% and 1.5% urea) for two months, at the Agriculture and Veterinary Research Station, King Faisal University. Rabbits supplied with ration containing 1.5% urea had significant increase in body weight after 4 weeks than those fed 1% urea (1995±60 vs. 1829± 53 g) and 6 weeks than the 0.5% group (3175± 125 vs. 2235±50 g). Additionally, no significant trend was detected for the hemogram parameters, except for the RBC count of 0.5% urea group (6.48±0.21 x106) (P<0.05) and monocyte% for the 1% group (8.56±0.82x103) and 1.5% group (4.99±1.38x103) (P<0.05). there were significant differences (P<0.05) among treatment groups 1.5% (5.75±0.57 g/dl) and 1% (4.13±0.57 g/dl) for tota...

Research paper thumbnail of Body Condition Scores at Calving and Their Association with Dairy Cow Performance and Health in Semiarid Environment Under Two Cooling Systems

Italian Journal of Animal Science, 2015

Research paper thumbnail of The acute and long-term safety evaluation of aqueous, methanolic and ethanolic extracts of Achillea fragrantissima

African Journal of Pharmacy and Pharmacology

This study was designed to explore the safety and side effects of the different extracts (water, ... more This study was designed to explore the safety and side effects of the different extracts (water, ethanolic and methanolic) of Achillea fragrantissima given acutely or on repeated doses (125 and 250 mg/kg) in rats. Acute and subchronic toxicity, as well as reproductive (fertility, embryotoxicity and teratogenecity, peri-and postnatal study) effects were recorded on treated and control rats. Daily administration of the plant extract revealed no significant changes on the body weights, heart rates, and other physiological parameters. The plant extract induced a significant increase in total proteins and globulins in rats. It did not induce any abnormal liver and kidney functional changes as demonstrated by serum biochemical analysis in rats. Interestingly, the plant extract induced a significant decrease in alkaline phosphatase (ALP), urea and creatinine. Significant decrease in blood glucose level was detected in animals receiving 250 mg/kg of the extract. The plant extract did not affect fertility. Dosed males showed comparable data with the controls when dosed at 250 mg/kg b.wt. It did not cause any embryotoxic, teratogenic or any deleterious effects on the dosed females and their offspring. Litter size, survival rate and weight gain were comparable between groups. In conclusion, A. fragrantissima extract is a well tolerated substance and had a wide safety margin. The tested plant extracts did not induce any toxic effects even on repeated administration in rats for 2 months. Additionally, no evidences of impaired fertility, or teratogenic potentials at higher doses up to several times the recommended maximum human doses were detected.

Research paper thumbnail of An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues

5th International Poultry Conference …, 2009

... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet hum... more ... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet human needs” An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues Tarik Rabie 1 , Mohamed Mandour 2 , Hassan Zeweil 3 ...

Research paper thumbnail of An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues

5th International Poultry Conference …, 2009

... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet hum... more ... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet human needs” An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues Tarik Rabie 1 , Mohamed Mandour 2 , Hassan Zeweil 3 ...

Research paper thumbnail of Effect of dietary chromium supplementation on productive and reproductive performance of early lactating dairy cows under heat stress

Journal of Animal Physiology and Animal Nutrition, 2010

One hundred and twenty early lactating Holstein dairy cows were assigned to investigate the effec... more One hundred and twenty early lactating Holstein dairy cows were assigned to investigate the effect of dietary chromium (Cr) supplementation (0 or 6 mg Cr/head/day from organic preparation) on the productive and reproductive performance as well as on some blood serum parameters under heat stress (35-40°C). Cows received treatment from 3 weeks pre-partum through 12 weeks post-partum. Chromium supplemented diet had lower body weight loss and improved dry matter intake with consequent reduction in the energy balance deficits during the first period after calving when compared with the control group. Chromium supplementation increased (p < 0.05) milk yield by 6.7%, 12.3% and 16.5% at 4, 8 and 12 weeks post-partum, respectively, whereas milk composition and milk to feed ratio were unaffected (p > 0.05) when compared with the control. Moreover, dietary Cr supplementation had no effect (p > 0.05) on blood serum glucose, calcium and phosphorus concentrations. A reduction (p < 0.05) of non-esterified fatty acids at 1 week pre-partum, 2 and 4 weeks post-partum was also observed. Serum insulin concentration increased whereas cortisol concentration decreased, when compared with the control group, throughout the whole experimental period. Also Cr supplementation showed a trend towards improving reproductive performance as indicated by increased percentage of pregnant cows in the first 28 days of breeding. It could be concluded that dietary Cr supplementation at level of 6 mg/head/day may offer a potential protective management practice to lessen the effect of heat stress in dairy cattle.

Research paper thumbnail of Selection for wingbone breaking strength in cage-reared broilers /

Research paper thumbnail of Neurobehavioural Effect of Zinc Oxide Nanoparticles and its Conventional Form on Adult Male Rats and Their Pups

Alexandria Journal of Veterinary Sciences, 2020

Zinc is involved in many protein structures, which had a role in some neurological functions. The... more Zinc is involved in many protein structures, which had a role in some neurological functions. The present study was designed to investigate the effect of zinc oxide nanoparticles and its conventional one on memory and learning ability of adult male rats and their offspring. Thirty adult male rats were randomly distributed into 5 groups (6 rats, each). First group (control group) was injected with Tween 80 (10%), second and third groups, received two doses of Zinc oxide nanoparticles (ZnONPs), 5 and 10 mg/kg bwt respectively. Fourth and fifth groups, administrated two doses of conventional Zinc oxide (CZnO), 5 and 10 mg/kg bwt, respectively. Rats in all groups were injected intraperitoneally by 1ml/rat for 16 days period, every other day. A series of neurobehavioural tests including open field, elevated plus maze and Morris water maze test were conducted. The obtained results indicated that the ZnONPs lower dose improved locomotor behaviour during open field test and had an anxiolytic effect in the elevated plus maze, but higher doses were not beneficial. It can be concluded that the zinc oxide nanoparticles are better than conventional zinc oxide.

Research paper thumbnail of Bee pollen and propolis as dietary supplements for rabbit: Effect on reproductive performance of does and on immunological response of does and their offspring

Journal of Animal Physiology and Animal Nutrition, 2019

To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 6... more To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 64 nulliparous NZW rabbits does were distributed among eight groups (eight animals/group). One unsupplemented group was the control; the other seven groups were supplemented, respectively, with zinc bacitracin (ZnB) at 100 mg, BP at 150 and 300 mg, Pro at 150 and 300 mg, BP+Pro at 150 and 300 mg of each three times/week, day after day continuously along eight parities. The BP300, Pro300 and BP+Pro150 groups had higher body weight of litter at birth and number of kids born alive. The BP supplementation at 150 mg increased plasma total protein and albumin than the control group. The BP or Pro at 150 mg decreased plasma T3 than the other groups except for BP+Pro150. The ZnB group had significantly greater T3 /T4 ratio compared to BP, Pro and BP+Pro at 150 mg. The BP+Pro150 group had less ALT than the control; BP300 and Pro 300 mg resulted in lower plasma AST than the groups Pro150 with or without BP and the control group. The plasma alkaline phosphatase of BP at 150 or 300 mg and BP+Pro150 was significantly greater than that of the Pro150 group. The BP+Pro300 group had higher WBCs than the other groups. In contrast, the lymphocytes were greater in the Pro and BP+Pro300 groups than in BP, Pro and BP+Pro at 150 mg. The groups supplemented with BP and BP+Pro at 150 and 300 mg had significantly greater SRBCs of doe rabbits and their offspring compared to the control and the ZnB group. The BP at 300 mg increased the serum albumin and α1 -globulin than the control group. The Pro300 group had greater serum α2 -globulin and β-globulin than the control group. The total globulin was significantly greater for the 300 mg propolis-supplemented groups than the control.

Research paper thumbnail of Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits

Journal of Animal Physiology and Animal Nutrition, 2019

Sixty-four nulliparous female rabbits were distributed among eight groups (eight animals/group). ... more Sixty-four nulliparous female rabbits were distributed among eight groups (eight animals/group). Group one was the unsupplemented control group; the other seven groups were supplemented with zinc bacitracin (ZnB) at 100 mg, or bee pollen (BP) and/or propolis (Pro) at 150 and 300 mg in a capsulated form, three times a week, day after day, continuously all over the experimental period. The experiment was run for eight parties; at each parity, 28 kids of each doe group (a total of 224 rabbits) were divided into two subgroups weaned, respectively, at 24 and 30 days of age. Thus, for each parity, there were 16 groups (eight does treatments × two weaning age, 14 rabbits per group). The growing rabbits fed the standard diets without supplements. The growth performance, the carcass traits, the liver and the spleen histology of rabbits were checked up to 90 days of age to find possible carryover effects of the supplements. The supplements had no significant effect on most of the growth performance at 90 days of age, but BP150 and BP+Pro300 increased the growth rate in comparison with ZnB group. The liver weight in the control, BP300 and Pro300 groups was higher than the ZnB one. The spleen weight was higher in the groups ZnB, BP150, Pro300 and BP+Pro300, followed by the control, BP300 and BP+Pro150 and thus Pro150. The heart % in the BP150 and Pro300 groups was higher than ZnB and BP+Pro150 groups. A lymphoid hyperplasia of splenic white pulp was observed in the BP+Pro groups, while propolis alone showed a mild activation of lymphobiosis. The Pro and BP groups showed the same picture of the control group exhibiting a hydropic degeneration of mostly hepatic cells, while the ZnB group exhibited adverse effect on the bile ducts featuring portal periductal inflammatory cells infiltration with epithelial hyperplasia reflecting chronic cholangitis. K E Y W O R D S bee pollen, carryover effects, growing rabbits, propolis How to cite this article: Attia YA, Bovera F, Abd-Elhamid AEE, et al. Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits. J

Research paper thumbnail of Effect of Feeding Graded Levels of Urea on Growing New Zealand White Rabbit Performance

Forty New Zealand white rabbits of about 1340 g live weight were used to evaluate the influence o... more Forty New Zealand white rabbits of about 1340 g live weight were used to evaluate the influence of diet supplemented with urea at different levels on growth performance, blood cellular and biochemical parameters. The rabbits were assigned into four dietary treatments control, 3 urea-supplemented groups (0.5%, 1% and 1.5% urea) for two months, at the Agriculture and Veterinary Research Station, King Faisal University. Rabbits supplied with ration containing 1.5% urea had significant increase in body weight after 4 weeks than those fed 1% urea (1995±60 vs. 1829± 53 g) and 6 weeks than the 0.5% group (3175± 125 vs. 2235±50 g). Additionally, no significant trend was detected for the hemogram parameters, except for the RBC count of 0.5% urea group (6.48±0.21 x106) (P<0.05) and monocyte% for the 1% group (8.56±0.82x103) and 1.5% group (4.99±1.38x103) (P<0.05). there were significant differences (P<0.05) among treatment groups 1.5% (5.75±0.57 g/dl) and 1% (4.13±0.57 g/dl) for tota...

Research paper thumbnail of Body Condition Scores at Calving and Their Association with Dairy Cow Performance and Health in Semiarid Environment Under Two Cooling Systems

Italian Journal of Animal Science, 2015

Research paper thumbnail of The acute and long-term safety evaluation of aqueous, methanolic and ethanolic extracts of Achillea fragrantissima

African Journal of Pharmacy and Pharmacology

This study was designed to explore the safety and side effects of the different extracts (water, ... more This study was designed to explore the safety and side effects of the different extracts (water, ethanolic and methanolic) of Achillea fragrantissima given acutely or on repeated doses (125 and 250 mg/kg) in rats. Acute and subchronic toxicity, as well as reproductive (fertility, embryotoxicity and teratogenecity, peri-and postnatal study) effects were recorded on treated and control rats. Daily administration of the plant extract revealed no significant changes on the body weights, heart rates, and other physiological parameters. The plant extract induced a significant increase in total proteins and globulins in rats. It did not induce any abnormal liver and kidney functional changes as demonstrated by serum biochemical analysis in rats. Interestingly, the plant extract induced a significant decrease in alkaline phosphatase (ALP), urea and creatinine. Significant decrease in blood glucose level was detected in animals receiving 250 mg/kg of the extract. The plant extract did not affect fertility. Dosed males showed comparable data with the controls when dosed at 250 mg/kg b.wt. It did not cause any embryotoxic, teratogenic or any deleterious effects on the dosed females and their offspring. Litter size, survival rate and weight gain were comparable between groups. In conclusion, A. fragrantissima extract is a well tolerated substance and had a wide safety margin. The tested plant extracts did not induce any toxic effects even on repeated administration in rats for 2 months. Additionally, no evidences of impaired fertility, or teratogenic potentials at higher doses up to several times the recommended maximum human doses were detected.

Research paper thumbnail of An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues

5th International Poultry Conference …, 2009

... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet hum... more ... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet human needs” An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues Tarik Rabie 1 , Mohamed Mandour 2 , Hassan Zeweil 3 ...

Research paper thumbnail of An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues

5th International Poultry Conference …, 2009

... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet hum... more ... “Utilisation of diversity in land use systems: Sustainable and organic approaches to meet human needs” An Affordable and Effortless Method for High-Throughput DNA Extraction from Animal Tissues Tarik Rabie 1 , Mohamed Mandour 2 , Hassan Zeweil 3 ...