Mohammad Badrud Duza - Academia.edu (original) (raw)
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Papers by Mohammad Badrud Duza
Indo American Journal of Pharmaceutical Research, 2015
International Journal of Applied Sciences and Biotechnology, 2015
The present study was carried out to demonstrate the optimization of growth conditions of bacteri... more The present study was carried out to demonstrate the optimization of growth conditions of bacteria with high cellulase activity. Cellulose degrading bacteria were isolated from soil samples collected from different areas of Guntur district, A.P. The bacteria were isolated using serial dilution and pour plate methods. The isolated bacteria were identified by morphological, biochemical and molecular procedures. The isolated bacterial species were screened for cellulase production in sub-merged fermentation process. The two tested bacterial species showed maximum yield for cellulase production. These two bacteria were identified as Bacillus anthracis and Ochrobactrum anthropi (YZ1). Supplementation of glucose, peptone, tyrosine and EDTA to the fermentation medium is favoured enzyme secretion. The optimum pH and temperature for the activity of crude enzyme was 8 and 45°C, respectively for Ochrobactrum anthropi (YZ1) while for Bacillus anthracis, it was 8 and 4°C, respectively.14% of ino...
IOSR Journal of Pharmacy and Biological Sciences, 2014
Aim: The aim of the present study is to estimate the optimum growth conditions of bacterial isola... more Aim: The aim of the present study is to estimate the optimum growth conditions of bacterial isolates TS11BP and TS2MCN having lipolytic activity. Materials and methods: Oil degrading bacteria were isolated from soil sample using serial dilution and pour plate method. Bacteria were further identified by morphological and biochemical tests and subjected to lipase production in 250 ml Erlenmeyer flask using Rhodamine olive oil medium and Tween 80 medium for 48h of fermentation period at 37 0 C with agitation speed of 150rpm in rotator shaker. Results: Different bacterial strains were isolated and screened for lipase production in submerged fermentation process. Among these two bacterial strains gave positive test for lipase production. The two strains were further characterized by biochemical and morphological tests and identified as Bacillus thuringiensis (TS11BP) and Achromobacter xylosoxidans (TS2MCN). Supplementation of various bacterial growth components such as Carbon, Nitrogen, Vitamin and Amino acids increased enzyme production. The optimum pH and temperature for the lipase activity of crude enzyme was 8 and 45 0 C for both the bacterial isolates. Maximum lipase production was recorded during 96hr of incubation period and 14% of inoculums level.
Acta Chromatographica, 2008
International Current Pharmaceutical Journal, Oct 3, 2012
A method for the determination of omeprazole in bulk and capsule dosage form by reverse phase hig... more A method for the determination of omeprazole in bulk and capsule dosage form by reverse phase high performance liquid chromatography has been developed. This is a simple, rapid, precise and an accurate method. The method was developed on a Novapak C18, (250 x 4.6 mm, 5µ) column using phosphate buffer (pH 7.4) and acetonitrile in the ratio of 60:40, v/v as a mobile phase which was pumped at a flow rate of 1.0 ml/min and detection was done at 302 nm. The retention time of the drug was found to be 7.71 min. The method was validated for accuracy, precision, linearity, specificity, robustness. The linearity was observed in the range of 20-60 ppm. The results of recovery studies indicated that the method was accurate. Hence the developed method was found to be suitable for the estimation of omeprazole in bulk and capsule dosage forms.
Indo American Journal of Pharmaceutical Research, 2015
International Journal of Applied Sciences and Biotechnology, 2015
The present study was carried out to demonstrate the optimization of growth conditions of bacteri... more The present study was carried out to demonstrate the optimization of growth conditions of bacteria with high cellulase activity. Cellulose degrading bacteria were isolated from soil samples collected from different areas of Guntur district, A.P. The bacteria were isolated using serial dilution and pour plate methods. The isolated bacteria were identified by morphological, biochemical and molecular procedures. The isolated bacterial species were screened for cellulase production in sub-merged fermentation process. The two tested bacterial species showed maximum yield for cellulase production. These two bacteria were identified as Bacillus anthracis and Ochrobactrum anthropi (YZ1). Supplementation of glucose, peptone, tyrosine and EDTA to the fermentation medium is favoured enzyme secretion. The optimum pH and temperature for the activity of crude enzyme was 8 and 45°C, respectively for Ochrobactrum anthropi (YZ1) while for Bacillus anthracis, it was 8 and 4°C, respectively.14% of ino...
IOSR Journal of Pharmacy and Biological Sciences, 2014
Aim: The aim of the present study is to estimate the optimum growth conditions of bacterial isola... more Aim: The aim of the present study is to estimate the optimum growth conditions of bacterial isolates TS11BP and TS2MCN having lipolytic activity. Materials and methods: Oil degrading bacteria were isolated from soil sample using serial dilution and pour plate method. Bacteria were further identified by morphological and biochemical tests and subjected to lipase production in 250 ml Erlenmeyer flask using Rhodamine olive oil medium and Tween 80 medium for 48h of fermentation period at 37 0 C with agitation speed of 150rpm in rotator shaker. Results: Different bacterial strains were isolated and screened for lipase production in submerged fermentation process. Among these two bacterial strains gave positive test for lipase production. The two strains were further characterized by biochemical and morphological tests and identified as Bacillus thuringiensis (TS11BP) and Achromobacter xylosoxidans (TS2MCN). Supplementation of various bacterial growth components such as Carbon, Nitrogen, Vitamin and Amino acids increased enzyme production. The optimum pH and temperature for the lipase activity of crude enzyme was 8 and 45 0 C for both the bacterial isolates. Maximum lipase production was recorded during 96hr of incubation period and 14% of inoculums level.
Acta Chromatographica, 2008
International Current Pharmaceutical Journal, Oct 3, 2012
A method for the determination of omeprazole in bulk and capsule dosage form by reverse phase hig... more A method for the determination of omeprazole in bulk and capsule dosage form by reverse phase high performance liquid chromatography has been developed. This is a simple, rapid, precise and an accurate method. The method was developed on a Novapak C18, (250 x 4.6 mm, 5µ) column using phosphate buffer (pH 7.4) and acetonitrile in the ratio of 60:40, v/v as a mobile phase which was pumped at a flow rate of 1.0 ml/min and detection was done at 302 nm. The retention time of the drug was found to be 7.71 min. The method was validated for accuracy, precision, linearity, specificity, robustness. The linearity was observed in the range of 20-60 ppm. The results of recovery studies indicated that the method was accurate. Hence the developed method was found to be suitable for the estimation of omeprazole in bulk and capsule dosage forms.