Mohammad Faranoush - Academia.edu (original) (raw)

Papers by Mohammad Faranoush

Despite multi-modal therapies for patients with malignant brain tumors, their median survival is ... more Despite multi-modal therapies for patients with malignant brain tumors, their median survival is < 2 year. Recently, NK cells provided to cancer immune surveillance through their direct natural cytotoxicity, by modulating dendritic cells to enhance the presentation of tumor antigens and regulating T-cell mediated antitumor responses. However, the success of this treatment modality in brain tumors is unclear. The main reasons are the brain tumor microenvironment, approach of NK cell preparation and administration and the donor selection. Our, previous study showed that intracranial injection of activated haploidentical NK cells resulted in the eradication of glioblastoma tumor masses in the animal model. The animals had no evidence of tumor recurrence after treatment, and all tumor-related complications resolved after treatment. Therefore, in the present study, we evaluated the safety of intra surgical cavity or intra cerebrospinal fluid (CSF) Injection of ex vivo activated haploi...

Scientific Journal of Iran Blood Transfus Organ, 2006

Background and Objectives Diversity of heavy chain immunoglobulin (IgH) and T-cell receptor (TCR)... more Background and Objectives Diversity of heavy chain immunoglobulin (IgH) and T-cell receptor (TCR) molecules is generated during Band T-lymphocyte differentiation through the rearrangement of variable (V), diversity (D), junction (J) and constant (C) gene segments. Additionally, random insertion and deletions of nucleotides between gene segments make unique sequences which are cell or clone specific. Lymphoid leukemia cells are similar to normal precursors and have rearranged IgH, Igκ and TCR (cross-lineage rearrangement) genes which can be used as a marker of clonality and evaluation of minimal residual disease (MRD). The purpose of this study is to evaluate the pattern of TCRδ/γ gene rearrangements using polymerase chain reaction (PCR) in B-precursor acute lymphoblastic leukemia (ALL) in Iranian children. Materials and Methods In our prospective study, bone marrow aspirates of 183 children including B-precursor ALLs and with diagnosis of acute leukemia were collected at admission b...

Phytotherapy Research, 2020

Berberine is a natural isoquinoline alkaloid that has been shown to inhibit the proliferation and... more Berberine is a natural isoquinoline alkaloid that has been shown to inhibit the proliferation and induce apoptosis in a wide variety of tumor cells. However, the action mechanism of berberine in CLL cells is unknown. The previous study has shown that berberine leads to reduced viability and elevated levels of apoptosis in PBMCs of CLL patients. CLL cells are characterized by remarkable expression of Bcl‐2 and ROR1 which leads to activation and survival and increases disease progression in patients. High‐level expression of miR‐21 in patients with CLL is associated with a higher risk of death. Here we investigated the anticancer effects of berberine upon peripheral blood mononuclear cells (PBMCs) of CLL patients. To evaluate the expression of anti‐apoptotic proteins and ROR1 using flow cytometry and western blot, PBMCs were treated with 25 μM of berberine for 24 hr. The expression levels of mir‐21 were evaluated by real‐time PCR. Examination of treated cells demonstrated that berberi...

Journal of Cellular Physiology, 2020

Chronic lymphocyte leukemia (CLL) is a B‐cell malignancy resisted to apoptosis. Recently, some st... more Chronic lymphocyte leukemia (CLL) is a B‐cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL‐27) can reduce B‐cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL‐27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL‐27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL‐27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL‐27 and IL‐27 receptor were determined using flow cytometry and real‐time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL‐27 on B cells of patients with CLL, Annexin V‐FITC and 7‐AAD (BioLegend) fluorescent dyes were used. In addition, the IL‐27 effect on...

Pediatric Hematology and Oncology, 2018

Background: The scarcity of information on pediatric ependymoma in Iran motivated this study. Our... more Background: The scarcity of information on pediatric ependymoma in Iran motivated this study. Our main objectives were to determine outcomes, identify clinical management challenges at a nongovernment hospital in Iran, and devise guidelines for improving care. Procedure: A retrospective chart review was performed for pediatric patients with ependymoma who were younger than 15 years and treated at MPCTRC between 2007 and 2015. Records included patient demographics, treatment regimens used, duration of follow-up, and outcomes. Clinical outcomes [ie, 3-year overall survival (OS) and progression-free survival (PFS)] were determined based on the age at diagnosis (younger or older than 3 years) by using the Kaplan-Meier method. Results: In total, 73 eligible patients were enrolled; 20 patients were in the younger group, and 53 were in the older group. The majority (91.8%, n = 67) of patients underwent initial gross-total or partial surgical resection, and 6 (8.2%) had a biopsy. Twenty-one patients experienced ependymoma recurrence. The median time to relapse was 1 year. The median duration of follow-up and PFS were 25 and 17 months, respectively. The 3-year OS and PFS were 61% and 59.5%, respectively. At the time of this project, 27 patients had died, and 35 were alive with no evidence of disease. Conclusion: Our study demonstrated inferior outcomes of Iranian children with ependymoma. To improve our care for these children, a paradigm shift must occur that includes radiation therapy as standard of care, second-look surgery, a multidisciplinary team approach, and potentially twinning initiatives.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery, Jan 6, 2017

The clinical management of pediatric medulloblastoma requires a multidisciplinary approach, which... more The clinical management of pediatric medulloblastoma requires a multidisciplinary approach, which can be challenging, especially in low- and middle-income countries. The aim of this study was to identify current challenges and describe the treatment and outcomes of Iranian pediatric patients with medulloblastoma who were referred to our center in Tehran, Iran. Our retrospective review included 126 patient records from April 2007 to May 2015. The records were analyzed for epidemiologic features, treatment modalities, overall survival, and progression-free survival. Data were analyzed using SPSS 22.0 software. Median age at diagnosis was 6 years (male:female ratio, 2.3:1). At the time of diagnosis, 7 patients were 2 years or younger, and 76 (60.3%) were categorized as having high-risk disease. Overall, 100 patients had gross or near-total surgical resection. Cerebral spinal fluid involvement was detected in 22.2% of the patients tested, and spinal involvement was detected in 25% of th...

Blood Coagulation & Fibrinolysis, 2017

Health-related quality of life (HRQOL) assessment should be part of the regular clinical assessme... more Health-related quality of life (HRQOL) assessment should be part of the regular clinical assessment of persons with hemophilia. This study assessed quality of life of severe hemophilia patients (Type A) in Tehran comprehensive hemophilia care center. This cross-sectional study was done in 2016 and 84 men aged over 20 years with severe hemophilia (Type A) were assessed. All patients have been treated over 10 years at the comprehensive hemophilia care center, Iran. The HRQOL assessment includes the A36 Hemophilia-QOL questionnaire, sociodemographical and clinical characteristics. The overall HRQOL mean (WSD) in the present sample was 69.1 W 26.0 (A36Hemofilia-QoL Global Index). The mean (WSD) scores according the domains were: physical health (15.0 W 5.7), daily activities (6.7 W 3.8), joint damage (4.2 W 2.9), pain (3.0 W 1.9), social activities and relationships (10.7 W 5.8) and emotional function (9.2 W 5.0) classified moderate-to-poor state. In contrast with treatment satisfaction (4.8 W 1.6), treatment difficulties (9.5 W 3.4) and mental health (6.2 W 3.3), those were all in moderate-to-good state. Patients with higher education levels had better quality of life in the area of anxiety (P U 0.034), mental health (P U 0.007), social activities and personal relationships (P U 0.002). In general, poor quality of life of patients was 13.1%, 42.9% weak-tomoderate, 38.1% moderate-to-good and 6% good. It was shown that over 50% of patients had low-to-moderate HRQOL in the domains, such as physical health, daily activities, joint damage, pain, social activities and personal relationships. Efforts are necessary to improve their HRQOL.

Pediatric Hematology and Oncology, 2008

Thalassemia is common in Iran. Appropriate therapy for this disease includes a regular blood tran... more Thalassemia is common in Iran. Appropriate therapy for this disease includes a regular blood transfusion and chelation therapy. However, in this approach patients will inevitably experience side effects, particularly iron overloads in critical organs, including heart, ductless glands, and liver. This study attempted to determine prevalence of adenoidal abnormality between Iranian thalassemia patients for prediagnosis and to offer necessary medical measures. This is a descriptive nonrandomized study and included all the patients suffering from thalassemia major referring to medical centers linked with the Iranian blood transfusion organization from January 2004 to January 2005. All patients were sampled for CBC, FBS, 2-h BS, HbAlC, liver function, renal function, and endocrine disease. Initially, reports of adenoidal experiments as well as other associated parameters were provided from medical records. A total of 437 patients enrolled in the study: 5.4% suffered from diabetes, 1% had hypothyroid, and 1 person showed hypoparathyroidism. The mean levels of ferritin in diabetic and nondiabetic groups and hypothyroid and nonhypothyroid groups were not significantly different. The mean levels of ferritin among various age groups also were not significantly different. Results of a comparison between present research and similar studies conducted throughout Iran and those performed abroad on adenoidal abnormalities in thalassemia patients show that subject patients of this study statistically suffered from fewer difficulties than diabetes patients in other studies.

ABSTRACT BACKGROUND : Anemia is a major nutritional problem of immense public health affecting pe... more ABSTRACT BACKGROUND : Anemia is a major nutritional problem of immense public health affecting persons of all ages , sex and economic group .Iron deficiency anemia (IDA ) is a most common hematologic problem of infancy and childhood . IDA affects on growth and intelectual development of children . Thus diagnosis and treatment of this disease is very important . RBC counts in IDA are usually normal but in thalassemia syndrome that is higher than normal . The purpose of this study was to evaluate frequency of high RBC count ( RBC count &gt; 5*10 6 /mcl ) in infants and young childrenwith IDA . METHOD and MATERIALS : A total of 53 infant and young children aged 6.0 to 72.0 months with nutritional IDA without a history of any bleeding disorder were the subject of this study .The patients were divided into three groups according to the severity of anemia .Group A1 children had Hb values 8 gr /dl or less ( severe anemia ) , Group A2 8.1 to 10.0 gr / dl ( moderate anemia ) and group A3 10.1 to 11.0 gr / dl ( mild anemia ) .All cases received oral iron ( 3-6 mg / kg/ day ) for 12 weeks . complete blood counts (CBC ) were done weekly for 2 weeks and monthly during rest of treatment . RESULTS : Atotal of 25 of the 53 patients (47.1%) had a high erythrocyte count. Of the 53 patients 24 in group A1 ( Mean of Hb 6.63 gr/dl ) ,15 in A2 (Mean Hb 9.36 gr/dl ) and 14 in A3 (Mean Hb 10.7 gr/dl ) . The frequency of high erythrocyte count was 33.3% , 60% and 57.1% in groups A1 , A2 and A3 repectively . The patients in high erythrocyte count had significantly higher Hb and Hct ( group A1 compare to A2 and A3 ) . In normal RBC counts ( Mean 4.24 *106/mcl and Hb 9.04 gr/dl ) was compared to high RBC count (Mean 5.4 * 10 6/mcl and Hb 9.8 gr/dl ) significant differences between MCV (57.7 vs 62.1 fl ) (p&lt;0.05).After 12wk treatment with oral iron were occurred significant differences in MCHC , Hb , Hct , MCH ( P&lt; 0.05 ). CONCLUSION : A high erythrocyte count is common feature of IDA in infants and young children with an increasing frequency from severe to moderate to mild anemia . High erythrocyte count cannot be regardedas a reliable preliminary parameter in differetiating IDA from thalassemia in infant and children aged up to 72 months . KEY WORDS : Iron deficiency anemia , Infancy , Childhood , Erythrocyte

Page 1. ﻲﻤﻠﻋ ﻪﻠﺠﻣ نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد - ﺪﻠﺟ 9 هرﺎﻤﺷ ، 4) ﻲﭘﺎﻴﭘ 28( ، نﺎﺘﺴﺑﺎﺗ 1387 329 ﻞﻣاﻮﻋ ... more Page 1. ﻲﻤﻠﻋ ﻪﻠﺠﻣ نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد - ﺪﻠﺟ 9 هرﺎﻤﺷ ، 4) ﻲﭘﺎﻴﭘ 28( ، نﺎﺘﺴﺑﺎﺗ 1387 329 ﻞﻣاﻮﻋ طﺎﺒﺗرا ﻲﺳرﺮﺑ يﺰﻳﺮﻧﻮﺧ تﺪﺷ ﺎﺑ ﻚﻴﻠﻴﻓﻮﺒﻣوﺮﺗ ﻪﺑ ﻼﺘﺒﻣ نارﺎﻤﻴﺑ رد ﻲﻠﻴﻓﻮﻤﻫ A ﺪﻳﺪﺷ شﻮﻧاﺮﻓ ﺪﻤﺤﻣ 1 (MD) ﻲﻳﺎﻧاد ﺪﻳﻮﻧ ، 1* (MD) ﻲﻧﺎـﺑﺮﻗ ﺐـﻫار ، 2 (Ph.D) ﻲﻧﺎـﻤﺣر ﻪـﺑﻮﺒﺤﻣ ، 3 (MD) ، ﻲﺑذﺎـﺟ ﺪـﻤﺤﻣ 3 (M.Sc) ، ...

Wilms tumor is the most common childhood renal tumor accounting for about 6% of pediatric maligna... more Wilms tumor is the most common childhood renal tumor accounting for about 6% of pediatric malignant disease. Most patients with Wilms tumor can be cured with treatment and subsequently lead normal life. The multidisciplinary management of Wilms tumor has resulted striking improvement in survival of more than 85% nowadays and has become a paradigm for successful cancer therapy. We describe the results of patients treated according to National Wilms Tumor studies (NWTS) 3-5, with surgical staging, Central pathology review and multimodality treatment. This is a historical cohort study on the all patients who had Wilms tumor. We used the existing files of all patients who had admitted to Ali Asghar Children's hospital with Wilms tumor in the years of 1990-2003. The patients evaluated for age, sex, histologic type of cancer, metastasis, outlook of relapse and outcome after 5 years from diagnosis. We analyzed 175 files of Wilms tumor. They are 49.7% are male and 50.3% female. Mean age (±S.D.) of patients at diagnosis was 3.8±0.4 year. The 5-year survival rate of these patients was approximately 76±4%. History of cancer in first degree of relative was 11.5% and family marriage was 36.4%. Tumor involvement were 45.3% in right kidney, 51.5% in left kidney and both kidney involvement in 3.2%. Congenital anomalies in association with Wilms tumor were urologic problem (1.5%), hemihypertrophy (0.5%), sporadic aniridia (0.5%) and without abnormalities (97.5%). Histologic type of tumor were 32.6% favorable, 65.2% unfavorable and 2.2% intermediate. Stage II and III were the most common (35.4%, 32.4%, respectively). Tumor relapse were occurred in 25.4%.

Hematology/Oncology Clinics of North America, 2013

Considerable progress has been made in recent years in understanding of the genetic basis for con... more Considerable progress has been made in recent years in understanding of the genetic basis for congenital neutropenia syndromes. With the advent of high-throughput genomic analyzing technologies, the underlying genetic causes of other congenital neutropenia syndromes are expected to be resolved in the near future. This knowledge will provide the foundation for genotype-phenotype correlations for infection susceptibility, response to therapy, and risk of malignant transformation, enabling optimal care for individual patients depending on their molecular pathophysiology. It is hoped that these investigations will enable the development of tailored molecular therapies to specifically correct the aberrant signaling cascades.

Iranian Biomedical Journal, 2021

Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unc... more Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of HCMV and EBV infections in patients with GBM. Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.

For people with Bombay phenotype due to the expression of strong IgM Anti H in their sera, transf... more For people with Bombay phenotype due to the expression of strong IgM Anti H in their sera, transfusion with ABO group O blood will cause acute hemolytic reactions. Therefore, it is important to detect them. Case Following O blood type transfusion to a blood group O Rh positive patient with multiple trauma, acute hemolytic transfusion reaction happened. The reaction was recognized by the trained nurse in the haemovigilance system. In serologic studies, a high level of anti-H, anti-A, and anti-B was detected in the patient blood. Conclusions This case increased the number of Bombay phenotypes in Iran to 16. Therefore, using antibody screening test and existence of trained nurses and physicians with haemovigilance system in order exact management of transfusion reactions should be considered necessary.

Background: Activated normal platelets undergo many biochemical and morphological changes, some o... more Background: Activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. Platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. This study was conducted to determine microparticles formation and platelet shrinkage in Glanzmann thrombasthenia upon activation. Patients and Methods: Platelets from twelve unrelated type I Glanzmann thrombasthenia patients were examined as washed platelets. Calcium ionophore A23187 was used as agonist to activate the platelets. Flow cytometry was applied to measure platelet-derived micro particles (forward scatter; events <1.0 µm size), and platelet shrinkage (mean-FSC). Anti-CD42b was used as platelet specific marker to distinguish platelets from other likely particles. Annexin A5 Alexa Fluor was used to determine phosphatidylserine exposure and confirm platelet activati...

Journal of AIDS & Clinical Research, 2013

Background and Objectives Several hereditary and acquired risk factors for thrombosis are known. ... more Background and Objectives Several hereditary and acquired risk factors for thrombosis are known. Among the genetic factors, PAI-1 4G/5G polymorphism can be noted. This study was done to investigate the association of 4G/5G polymorphism in PAI-1 gene and thrombosis in coronary arteries. Materials and Methods Sixty one patients with the history of thrombosis in coronary arteries and 92 healthy blood donors participated as the control in our study. After DNA extraction from leucocytes based on the selective detergent-mediated DNA precipitation from crude lysate, PCR was performed using ARMS technique. Single and multivariate analyses were applied to adjust for potentially confounding factors using SPSS 19 software. The data were also compared with those of the other similar studies. Results The results showed 61 patients with history of coronary artery thrombosis for PAI-1 with the values of 24.6%, 45.9%, and 29.5% for 4G/4G, 4G/5G, and 5G/5G, respectively; the values for 92 healthy blood donors were evaluated to be 20.7%, 42.2%, and 37% in order. The polymorphism studied was not significantly different between cases and controls. Single and multivariate analyses show a significant difference for the conventional risk factors for coronary artery disease between patients and healthy controls (P value: 0.001). Conclusions We found no association between arterial thrombosis and the 4G/4G genotype for PAI-1 gene in Iranian population of the current study.

Despite multi-modal therapies for patients with malignant brain tumors, their median survival is ... more Despite multi-modal therapies for patients with malignant brain tumors, their median survival is < 2 year. Recently, NK cells provided to cancer immune surveillance through their direct natural cytotoxicity, by modulating dendritic cells to enhance the presentation of tumor antigens and regulating T-cell mediated antitumor responses. However, the success of this treatment modality in brain tumors is unclear. The main reasons are the brain tumor microenvironment, approach of NK cell preparation and administration and the donor selection. Our, previous study showed that intracranial injection of activated haploidentical NK cells resulted in the eradication of glioblastoma tumor masses in the animal model. The animals had no evidence of tumor recurrence after treatment, and all tumor-related complications resolved after treatment. Therefore, in the present study, we evaluated the safety of intra surgical cavity or intra cerebrospinal fluid (CSF) Injection of ex vivo activated haploi...

Scientific Journal of Iran Blood Transfus Organ, 2006

Background and Objectives Diversity of heavy chain immunoglobulin (IgH) and T-cell receptor (TCR)... more Background and Objectives Diversity of heavy chain immunoglobulin (IgH) and T-cell receptor (TCR) molecules is generated during Band T-lymphocyte differentiation through the rearrangement of variable (V), diversity (D), junction (J) and constant (C) gene segments. Additionally, random insertion and deletions of nucleotides between gene segments make unique sequences which are cell or clone specific. Lymphoid leukemia cells are similar to normal precursors and have rearranged IgH, Igκ and TCR (cross-lineage rearrangement) genes which can be used as a marker of clonality and evaluation of minimal residual disease (MRD). The purpose of this study is to evaluate the pattern of TCRδ/γ gene rearrangements using polymerase chain reaction (PCR) in B-precursor acute lymphoblastic leukemia (ALL) in Iranian children. Materials and Methods In our prospective study, bone marrow aspirates of 183 children including B-precursor ALLs and with diagnosis of acute leukemia were collected at admission b...

Phytotherapy Research, 2020

Berberine is a natural isoquinoline alkaloid that has been shown to inhibit the proliferation and... more Berberine is a natural isoquinoline alkaloid that has been shown to inhibit the proliferation and induce apoptosis in a wide variety of tumor cells. However, the action mechanism of berberine in CLL cells is unknown. The previous study has shown that berberine leads to reduced viability and elevated levels of apoptosis in PBMCs of CLL patients. CLL cells are characterized by remarkable expression of Bcl‐2 and ROR1 which leads to activation and survival and increases disease progression in patients. High‐level expression of miR‐21 in patients with CLL is associated with a higher risk of death. Here we investigated the anticancer effects of berberine upon peripheral blood mononuclear cells (PBMCs) of CLL patients. To evaluate the expression of anti‐apoptotic proteins and ROR1 using flow cytometry and western blot, PBMCs were treated with 25 μM of berberine for 24 hr. The expression levels of mir‐21 were evaluated by real‐time PCR. Examination of treated cells demonstrated that berberi...

Journal of Cellular Physiology, 2020

Chronic lymphocyte leukemia (CLL) is a B‐cell malignancy resisted to apoptosis. Recently, some st... more Chronic lymphocyte leukemia (CLL) is a B‐cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL‐27) can reduce B‐cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL‐27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL‐27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL‐27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL‐27 and IL‐27 receptor were determined using flow cytometry and real‐time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL‐27 on B cells of patients with CLL, Annexin V‐FITC and 7‐AAD (BioLegend) fluorescent dyes were used. In addition, the IL‐27 effect on...

Pediatric Hematology and Oncology, 2018

Background: The scarcity of information on pediatric ependymoma in Iran motivated this study. Our... more Background: The scarcity of information on pediatric ependymoma in Iran motivated this study. Our main objectives were to determine outcomes, identify clinical management challenges at a nongovernment hospital in Iran, and devise guidelines for improving care. Procedure: A retrospective chart review was performed for pediatric patients with ependymoma who were younger than 15 years and treated at MPCTRC between 2007 and 2015. Records included patient demographics, treatment regimens used, duration of follow-up, and outcomes. Clinical outcomes [ie, 3-year overall survival (OS) and progression-free survival (PFS)] were determined based on the age at diagnosis (younger or older than 3 years) by using the Kaplan-Meier method. Results: In total, 73 eligible patients were enrolled; 20 patients were in the younger group, and 53 were in the older group. The majority (91.8%, n = 67) of patients underwent initial gross-total or partial surgical resection, and 6 (8.2%) had a biopsy. Twenty-one patients experienced ependymoma recurrence. The median time to relapse was 1 year. The median duration of follow-up and PFS were 25 and 17 months, respectively. The 3-year OS and PFS were 61% and 59.5%, respectively. At the time of this project, 27 patients had died, and 35 were alive with no evidence of disease. Conclusion: Our study demonstrated inferior outcomes of Iranian children with ependymoma. To improve our care for these children, a paradigm shift must occur that includes radiation therapy as standard of care, second-look surgery, a multidisciplinary team approach, and potentially twinning initiatives.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery, Jan 6, 2017

The clinical management of pediatric medulloblastoma requires a multidisciplinary approach, which... more The clinical management of pediatric medulloblastoma requires a multidisciplinary approach, which can be challenging, especially in low- and middle-income countries. The aim of this study was to identify current challenges and describe the treatment and outcomes of Iranian pediatric patients with medulloblastoma who were referred to our center in Tehran, Iran. Our retrospective review included 126 patient records from April 2007 to May 2015. The records were analyzed for epidemiologic features, treatment modalities, overall survival, and progression-free survival. Data were analyzed using SPSS 22.0 software. Median age at diagnosis was 6 years (male:female ratio, 2.3:1). At the time of diagnosis, 7 patients were 2 years or younger, and 76 (60.3%) were categorized as having high-risk disease. Overall, 100 patients had gross or near-total surgical resection. Cerebral spinal fluid involvement was detected in 22.2% of the patients tested, and spinal involvement was detected in 25% of th...

Blood Coagulation & Fibrinolysis, 2017

Health-related quality of life (HRQOL) assessment should be part of the regular clinical assessme... more Health-related quality of life (HRQOL) assessment should be part of the regular clinical assessment of persons with hemophilia. This study assessed quality of life of severe hemophilia patients (Type A) in Tehran comprehensive hemophilia care center. This cross-sectional study was done in 2016 and 84 men aged over 20 years with severe hemophilia (Type A) were assessed. All patients have been treated over 10 years at the comprehensive hemophilia care center, Iran. The HRQOL assessment includes the A36 Hemophilia-QOL questionnaire, sociodemographical and clinical characteristics. The overall HRQOL mean (WSD) in the present sample was 69.1 W 26.0 (A36Hemofilia-QoL Global Index). The mean (WSD) scores according the domains were: physical health (15.0 W 5.7), daily activities (6.7 W 3.8), joint damage (4.2 W 2.9), pain (3.0 W 1.9), social activities and relationships (10.7 W 5.8) and emotional function (9.2 W 5.0) classified moderate-to-poor state. In contrast with treatment satisfaction (4.8 W 1.6), treatment difficulties (9.5 W 3.4) and mental health (6.2 W 3.3), those were all in moderate-to-good state. Patients with higher education levels had better quality of life in the area of anxiety (P U 0.034), mental health (P U 0.007), social activities and personal relationships (P U 0.002). In general, poor quality of life of patients was 13.1%, 42.9% weak-tomoderate, 38.1% moderate-to-good and 6% good. It was shown that over 50% of patients had low-to-moderate HRQOL in the domains, such as physical health, daily activities, joint damage, pain, social activities and personal relationships. Efforts are necessary to improve their HRQOL.

Pediatric Hematology and Oncology, 2008

Thalassemia is common in Iran. Appropriate therapy for this disease includes a regular blood tran... more Thalassemia is common in Iran. Appropriate therapy for this disease includes a regular blood transfusion and chelation therapy. However, in this approach patients will inevitably experience side effects, particularly iron overloads in critical organs, including heart, ductless glands, and liver. This study attempted to determine prevalence of adenoidal abnormality between Iranian thalassemia patients for prediagnosis and to offer necessary medical measures. This is a descriptive nonrandomized study and included all the patients suffering from thalassemia major referring to medical centers linked with the Iranian blood transfusion organization from January 2004 to January 2005. All patients were sampled for CBC, FBS, 2-h BS, HbAlC, liver function, renal function, and endocrine disease. Initially, reports of adenoidal experiments as well as other associated parameters were provided from medical records. A total of 437 patients enrolled in the study: 5.4% suffered from diabetes, 1% had hypothyroid, and 1 person showed hypoparathyroidism. The mean levels of ferritin in diabetic and nondiabetic groups and hypothyroid and nonhypothyroid groups were not significantly different. The mean levels of ferritin among various age groups also were not significantly different. Results of a comparison between present research and similar studies conducted throughout Iran and those performed abroad on adenoidal abnormalities in thalassemia patients show that subject patients of this study statistically suffered from fewer difficulties than diabetes patients in other studies.

ABSTRACT BACKGROUND : Anemia is a major nutritional problem of immense public health affecting pe... more ABSTRACT BACKGROUND : Anemia is a major nutritional problem of immense public health affecting persons of all ages , sex and economic group .Iron deficiency anemia (IDA ) is a most common hematologic problem of infancy and childhood . IDA affects on growth and intelectual development of children . Thus diagnosis and treatment of this disease is very important . RBC counts in IDA are usually normal but in thalassemia syndrome that is higher than normal . The purpose of this study was to evaluate frequency of high RBC count ( RBC count &gt; 5*10 6 /mcl ) in infants and young childrenwith IDA . METHOD and MATERIALS : A total of 53 infant and young children aged 6.0 to 72.0 months with nutritional IDA without a history of any bleeding disorder were the subject of this study .The patients were divided into three groups according to the severity of anemia .Group A1 children had Hb values 8 gr /dl or less ( severe anemia ) , Group A2 8.1 to 10.0 gr / dl ( moderate anemia ) and group A3 10.1 to 11.0 gr / dl ( mild anemia ) .All cases received oral iron ( 3-6 mg / kg/ day ) for 12 weeks . complete blood counts (CBC ) were done weekly for 2 weeks and monthly during rest of treatment . RESULTS : Atotal of 25 of the 53 patients (47.1%) had a high erythrocyte count. Of the 53 patients 24 in group A1 ( Mean of Hb 6.63 gr/dl ) ,15 in A2 (Mean Hb 9.36 gr/dl ) and 14 in A3 (Mean Hb 10.7 gr/dl ) . The frequency of high erythrocyte count was 33.3% , 60% and 57.1% in groups A1 , A2 and A3 repectively . The patients in high erythrocyte count had significantly higher Hb and Hct ( group A1 compare to A2 and A3 ) . In normal RBC counts ( Mean 4.24 *106/mcl and Hb 9.04 gr/dl ) was compared to high RBC count (Mean 5.4 * 10 6/mcl and Hb 9.8 gr/dl ) significant differences between MCV (57.7 vs 62.1 fl ) (p&lt;0.05).After 12wk treatment with oral iron were occurred significant differences in MCHC , Hb , Hct , MCH ( P&lt; 0.05 ). CONCLUSION : A high erythrocyte count is common feature of IDA in infants and young children with an increasing frequency from severe to moderate to mild anemia . High erythrocyte count cannot be regardedas a reliable preliminary parameter in differetiating IDA from thalassemia in infant and children aged up to 72 months . KEY WORDS : Iron deficiency anemia , Infancy , Childhood , Erythrocyte

Page 1. ﻲﻤﻠﻋ ﻪﻠﺠﻣ نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد - ﺪﻠﺟ 9 هرﺎﻤﺷ ، 4) ﻲﭘﺎﻴﭘ 28( ، نﺎﺘﺴﺑﺎﺗ 1387 329 ﻞﻣاﻮﻋ ... more Page 1. ﻲﻤﻠﻋ ﻪﻠﺠﻣ نﺎﻨﻤﺳ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد - ﺪﻠﺟ 9 هرﺎﻤﺷ ، 4) ﻲﭘﺎﻴﭘ 28( ، نﺎﺘﺴﺑﺎﺗ 1387 329 ﻞﻣاﻮﻋ طﺎﺒﺗرا ﻲﺳرﺮﺑ يﺰﻳﺮﻧﻮﺧ تﺪﺷ ﺎﺑ ﻚﻴﻠﻴﻓﻮﺒﻣوﺮﺗ ﻪﺑ ﻼﺘﺒﻣ نارﺎﻤﻴﺑ رد ﻲﻠﻴﻓﻮﻤﻫ A ﺪﻳﺪﺷ شﻮﻧاﺮﻓ ﺪﻤﺤﻣ 1 (MD) ﻲﻳﺎﻧاد ﺪﻳﻮﻧ ، 1* (MD) ﻲﻧﺎـﺑﺮﻗ ﺐـﻫار ، 2 (Ph.D) ﻲﻧﺎـﻤﺣر ﻪـﺑﻮﺒﺤﻣ ، 3 (MD) ، ﻲﺑذﺎـﺟ ﺪـﻤﺤﻣ 3 (M.Sc) ، ...

Wilms tumor is the most common childhood renal tumor accounting for about 6% of pediatric maligna... more Wilms tumor is the most common childhood renal tumor accounting for about 6% of pediatric malignant disease. Most patients with Wilms tumor can be cured with treatment and subsequently lead normal life. The multidisciplinary management of Wilms tumor has resulted striking improvement in survival of more than 85% nowadays and has become a paradigm for successful cancer therapy. We describe the results of patients treated according to National Wilms Tumor studies (NWTS) 3-5, with surgical staging, Central pathology review and multimodality treatment. This is a historical cohort study on the all patients who had Wilms tumor. We used the existing files of all patients who had admitted to Ali Asghar Children's hospital with Wilms tumor in the years of 1990-2003. The patients evaluated for age, sex, histologic type of cancer, metastasis, outlook of relapse and outcome after 5 years from diagnosis. We analyzed 175 files of Wilms tumor. They are 49.7% are male and 50.3% female. Mean age (±S.D.) of patients at diagnosis was 3.8±0.4 year. The 5-year survival rate of these patients was approximately 76±4%. History of cancer in first degree of relative was 11.5% and family marriage was 36.4%. Tumor involvement were 45.3% in right kidney, 51.5% in left kidney and both kidney involvement in 3.2%. Congenital anomalies in association with Wilms tumor were urologic problem (1.5%), hemihypertrophy (0.5%), sporadic aniridia (0.5%) and without abnormalities (97.5%). Histologic type of tumor were 32.6% favorable, 65.2% unfavorable and 2.2% intermediate. Stage II and III were the most common (35.4%, 32.4%, respectively). Tumor relapse were occurred in 25.4%.

Hematology/Oncology Clinics of North America, 2013

Considerable progress has been made in recent years in understanding of the genetic basis for con... more Considerable progress has been made in recent years in understanding of the genetic basis for congenital neutropenia syndromes. With the advent of high-throughput genomic analyzing technologies, the underlying genetic causes of other congenital neutropenia syndromes are expected to be resolved in the near future. This knowledge will provide the foundation for genotype-phenotype correlations for infection susceptibility, response to therapy, and risk of malignant transformation, enabling optimal care for individual patients depending on their molecular pathophysiology. It is hoped that these investigations will enable the development of tailored molecular therapies to specifically correct the aberrant signaling cascades.

Iranian Biomedical Journal, 2021

Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unc... more Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of HCMV and EBV infections in patients with GBM. Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.

For people with Bombay phenotype due to the expression of strong IgM Anti H in their sera, transf... more For people with Bombay phenotype due to the expression of strong IgM Anti H in their sera, transfusion with ABO group O blood will cause acute hemolytic reactions. Therefore, it is important to detect them. Case Following O blood type transfusion to a blood group O Rh positive patient with multiple trauma, acute hemolytic transfusion reaction happened. The reaction was recognized by the trained nurse in the haemovigilance system. In serologic studies, a high level of anti-H, anti-A, and anti-B was detected in the patient blood. Conclusions This case increased the number of Bombay phenotypes in Iran to 16. Therefore, using antibody screening test and existence of trained nurses and physicians with haemovigilance system in order exact management of transfusion reactions should be considered necessary.

Background: Activated normal platelets undergo many biochemical and morphological changes, some o... more Background: Activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. Platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. This study was conducted to determine microparticles formation and platelet shrinkage in Glanzmann thrombasthenia upon activation. Patients and Methods: Platelets from twelve unrelated type I Glanzmann thrombasthenia patients were examined as washed platelets. Calcium ionophore A23187 was used as agonist to activate the platelets. Flow cytometry was applied to measure platelet-derived micro particles (forward scatter; events <1.0 µm size), and platelet shrinkage (mean-FSC). Anti-CD42b was used as platelet specific marker to distinguish platelets from other likely particles. Annexin A5 Alexa Fluor was used to determine phosphatidylserine exposure and confirm platelet activati...

Journal of AIDS & Clinical Research, 2013

Background and Objectives Several hereditary and acquired risk factors for thrombosis are known. ... more Background and Objectives Several hereditary and acquired risk factors for thrombosis are known. Among the genetic factors, PAI-1 4G/5G polymorphism can be noted. This study was done to investigate the association of 4G/5G polymorphism in PAI-1 gene and thrombosis in coronary arteries. Materials and Methods Sixty one patients with the history of thrombosis in coronary arteries and 92 healthy blood donors participated as the control in our study. After DNA extraction from leucocytes based on the selective detergent-mediated DNA precipitation from crude lysate, PCR was performed using ARMS technique. Single and multivariate analyses were applied to adjust for potentially confounding factors using SPSS 19 software. The data were also compared with those of the other similar studies. Results The results showed 61 patients with history of coronary artery thrombosis for PAI-1 with the values of 24.6%, 45.9%, and 29.5% for 4G/4G, 4G/5G, and 5G/5G, respectively; the values for 92 healthy blood donors were evaluated to be 20.7%, 42.2%, and 37% in order. The polymorphism studied was not significantly different between cases and controls. Single and multivariate analyses show a significant difference for the conventional risk factors for coronary artery disease between patients and healthy controls (P value: 0.001). Conclusions We found no association between arterial thrombosis and the 4G/4G genotype for PAI-1 gene in Iranian population of the current study.