Mohammed M U S T A F A Asaad (original) (raw)

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Papers by Mohammed M U S T A F A Asaad

Research paper thumbnail of Line Maze Solver Robot

Research paper thumbnail of Improving Business Marketing Using Logo Detection

Research paper thumbnail of Differential Isoniazid Response Pattern Between Active and Dormant Mycobacterium tuberculosis

Microbial Drug Resistance, 2021

Isoniazid (isonicotinic acid hydrazide, INH) is an effective frontline antituberculosis drug. INH... more Isoniazid (isonicotinic acid hydrazide, INH) is an effective frontline antituberculosis drug. INH targets several Mycobacterium tuberculosis processes, including mycolic acid biosynthesis, DNA synthesis, and redox potential. M. tuberculosis responds to INH stress by altering the expression level of crucial genes involved in various pathways. In this study, we summarize the induced gene expression pattern of active M. tuberculosis upon INH exposure. Most genes triggered by INH are involved in processes such as mycolic acid biosynthesis, a compensatory response, stress response, and drug efflux. These patterns are absent in dormant M. tuberculosis. The differential INH response pattern can inform future novel measures against M. tuberculosis.

Research paper thumbnail of Mycobacterium tuberculosis PPE10 (Rv0442c) alters host cell apoptosis and cytokine profile via linear ubiquitin chain assembly complex HOIP-NF-κB signaling axis

International Immunopharmacology, 2021

Tuberculosis caused by Mycobacterium tuberculosis infection remains one of the top ten causes of ... more Tuberculosis caused by Mycobacterium tuberculosis infection remains one of the top ten causes of deaths worldwide. M. tuberculosis genome devoted 10% capacity for highly repeated PE/PPE genes family. To explore the role of PPE10 in host-pathogen interaction, PPE10 encoding gene Rv0442c was heterologously expressed in the nonpathogenic M. smegmatis strain. PPE10 altered the bacterial cell surface properties, colony morphology, and biofilm formation. Ms_PPE10 showed more resistance to stress conditions such as diamide, and low pH, as well as higher survival within the macrophage. Moreover, the host's cell apoptosis was regulated via decreased expression of caspases, IL-1, IL-6, and TNF-α through the Linear Ubiquitin Chain Assembly Complex (LUBAC) HOIP-NF-κB signaling axis. The study revealed novel insights into the mechanism of action of the PPE family.

Research paper thumbnail of Mycobacterium tuberculosis Rv0580c Impedes the Intracellular Survival of Recombinant Mycobacteria, Manipulates the Cytokines, and Induces ER Stress and Apoptosis in Host Macrophages via NF-κB and p38/JNK Signaling

Pathogens, 2021

The Mycobacterium tuberculosis (M. tb) genome encodes a large number of hypothetical proteins, wh... more The Mycobacterium tuberculosis (M. tb) genome encodes a large number of hypothetical proteins, which need to investigate their role in physiology, virulence, pathogenesis, and host interaction. To explore the role of hypothetical protein Rv0580c, we constructed the recombinant Mycobacterium smegmatis (M. smegmatis) strain, which expressed the Rv0580c protein heterologously. We observed that Rv0580c expressing M. smegmatis strain (Ms_Rv0580c) altered the colony morphology and increased the cell wall permeability, leading to this recombinant strain becoming susceptible to acidic stress, oxidative stress, cell wall-perturbing stress, and multiple antibiotics. The intracellular survival of Ms_Rv0580c was reduced in THP-1 macrophages. Ms_Rv0580c up-regulated the IFN-γ expression via NF-κB and JNK signaling, and down-regulated IL-10 expression via NF-κB signaling in THP-1 macrophages as compared to control. Moreover, Ms_Rv0580c up-regulated the expression of HIF-1α and ER stress marker ge...

Research paper thumbnail of Estimation of fatality rate in Africa through the behavior of COVID-19 in Italy relevance to age profiles

The emergence and pandemic of COVID-19 has rapidly become a global concern. In Italy, on 27 March... more The emergence and pandemic of COVID-19 has rapidly become a global concern. In Italy, on 27 March 2020, there were 8165 deaths and 80539 confirmed cases of COVID-19. Demographic situations, like age profiles is reported to be the cause of high case fatality rate (CFR) in Italy. In Africa, the COVID-19 pandemic has not yet grasped epic proportion, but the estimation of CFR is still needed. We compared the CFR observed in Italy with the age profiles in 46 Africa countries and 2 territories which are already confirmed COVID-19 case. The estimation of the CFR in Africa ranges between (1.0%-5.4%) while in Italy is 10.1%. The five highest CFR countries and territories in Africa are Reunion (5.4%), Mauritius (5.1 %), Tunisia (3.9%), Seychelles (3.8%) and Morocco (3.3%). The last three countries with low CFR are Uganda (1.0%), Zambia (1.1%) and Angola (1.1%). The observed difference is related to the age profiles.

Research paper thumbnail of Methylation in Mycobacterium-host interaction and implications for novel control measures

Infection, Genetics and Evolution, 2020

Methylation epigenetically regulates many pivotal biological processes. Mycobacterium tuberculosi... more Methylation epigenetically regulates many pivotal biological processes. Mycobacterium tuberculosis, the pathogen of tuberculosis, can modulate host methylome. The methylated genes, sites, signaling pathway, chromatin remodeling, especially the immune related genes such as cytokines and chemokines, drug resistance and vaccines efficacy relevant genes were summarized in this paper. The results showed that methylation plays important roles in immune evasion, pathogenesis, persistence, disease progression, active, drug responder and non-responder. This will inform better practice for the development of new drugs and vaccines to eradicate tuberculosis.

Research paper thumbnail of Influence of Extract Derived In-Vitro Cell Suspension Cultures of Echinacea purpurea Against Some Immunosuppressive Effects

Research Journal of Pharmaceutical, Biological and Chemical Sciences

The present study investigates the therapeutic properties of in vitro E .purpurea cell suspension... more The present study investigates the therapeutic properties of in vitro E .purpurea cell suspension cultures against immunosuppressive effects of cyclophosphamide. Rats were assigned to four equal groups: normal control group, immune-suppressed groups: 2, 3 and 4 injected with cyclophosamide (50 mg/kg /day) i.m. for 3 consecutive days; group 2 kept as control positive, and the other two groups (3 & 4) were administered E.purpurea extract at doses 100 mg and 200 mg/kg, p.o., respectively & daily for 21 successive days. Blood samples were withdrawn twice; at the day 11and the 21 for blood cells count and the second for determination of cytokines level, γ-globulin, and MTT-value. Cyclophosphamide treated group showed significant decrease in total leucocytic count, anemia, and thrombocytopenia as well as humoral and cellular immunity markers; γ-globulin and MTT. E.purpurea treatment normalized the reduced blood cell elements in dose dependent manner. The extract inhibited production of TN...

Research paper thumbnail of Finite Groups with Quasinormal Subgroups of Prime Power Order

Acta Mathematica Hungarica, 2000

Let G be a finite group. A subgroup H is quasinormal in G if H permutes with every subgroup of G.... more Let G be a finite group. A subgroup H is quasinormal in G if H permutes with every subgroup of G. We examine the structure of G when certain abelian subgroups of prime power order are quasinormal in G.

Research paper thumbnail of On Generalized Hypercenter of a Finite Group

Communications in Algebra, 2001

Let G be a finite group. In this paper, we introduce the concept of super generalized supersolvab... more Let G be a finite group. In this paper, we introduce the concept of super generalized supersolvably embedded subgroup of a group G and give a new characterization of the generalized hypercenter of G .

Research paper thumbnail of Line Maze Solver Robot

Research paper thumbnail of Improving Business Marketing Using Logo Detection

Research paper thumbnail of Differential Isoniazid Response Pattern Between Active and Dormant Mycobacterium tuberculosis

Microbial Drug Resistance, 2021

Isoniazid (isonicotinic acid hydrazide, INH) is an effective frontline antituberculosis drug. INH... more Isoniazid (isonicotinic acid hydrazide, INH) is an effective frontline antituberculosis drug. INH targets several Mycobacterium tuberculosis processes, including mycolic acid biosynthesis, DNA synthesis, and redox potential. M. tuberculosis responds to INH stress by altering the expression level of crucial genes involved in various pathways. In this study, we summarize the induced gene expression pattern of active M. tuberculosis upon INH exposure. Most genes triggered by INH are involved in processes such as mycolic acid biosynthesis, a compensatory response, stress response, and drug efflux. These patterns are absent in dormant M. tuberculosis. The differential INH response pattern can inform future novel measures against M. tuberculosis.

Research paper thumbnail of Mycobacterium tuberculosis PPE10 (Rv0442c) alters host cell apoptosis and cytokine profile via linear ubiquitin chain assembly complex HOIP-NF-κB signaling axis

International Immunopharmacology, 2021

Tuberculosis caused by Mycobacterium tuberculosis infection remains one of the top ten causes of ... more Tuberculosis caused by Mycobacterium tuberculosis infection remains one of the top ten causes of deaths worldwide. M. tuberculosis genome devoted 10% capacity for highly repeated PE/PPE genes family. To explore the role of PPE10 in host-pathogen interaction, PPE10 encoding gene Rv0442c was heterologously expressed in the nonpathogenic M. smegmatis strain. PPE10 altered the bacterial cell surface properties, colony morphology, and biofilm formation. Ms_PPE10 showed more resistance to stress conditions such as diamide, and low pH, as well as higher survival within the macrophage. Moreover, the host's cell apoptosis was regulated via decreased expression of caspases, IL-1, IL-6, and TNF-α through the Linear Ubiquitin Chain Assembly Complex (LUBAC) HOIP-NF-κB signaling axis. The study revealed novel insights into the mechanism of action of the PPE family.

Research paper thumbnail of Mycobacterium tuberculosis Rv0580c Impedes the Intracellular Survival of Recombinant Mycobacteria, Manipulates the Cytokines, and Induces ER Stress and Apoptosis in Host Macrophages via NF-κB and p38/JNK Signaling

Pathogens, 2021

The Mycobacterium tuberculosis (M. tb) genome encodes a large number of hypothetical proteins, wh... more The Mycobacterium tuberculosis (M. tb) genome encodes a large number of hypothetical proteins, which need to investigate their role in physiology, virulence, pathogenesis, and host interaction. To explore the role of hypothetical protein Rv0580c, we constructed the recombinant Mycobacterium smegmatis (M. smegmatis) strain, which expressed the Rv0580c protein heterologously. We observed that Rv0580c expressing M. smegmatis strain (Ms_Rv0580c) altered the colony morphology and increased the cell wall permeability, leading to this recombinant strain becoming susceptible to acidic stress, oxidative stress, cell wall-perturbing stress, and multiple antibiotics. The intracellular survival of Ms_Rv0580c was reduced in THP-1 macrophages. Ms_Rv0580c up-regulated the IFN-γ expression via NF-κB and JNK signaling, and down-regulated IL-10 expression via NF-κB signaling in THP-1 macrophages as compared to control. Moreover, Ms_Rv0580c up-regulated the expression of HIF-1α and ER stress marker ge...

Research paper thumbnail of Estimation of fatality rate in Africa through the behavior of COVID-19 in Italy relevance to age profiles

The emergence and pandemic of COVID-19 has rapidly become a global concern. In Italy, on 27 March... more The emergence and pandemic of COVID-19 has rapidly become a global concern. In Italy, on 27 March 2020, there were 8165 deaths and 80539 confirmed cases of COVID-19. Demographic situations, like age profiles is reported to be the cause of high case fatality rate (CFR) in Italy. In Africa, the COVID-19 pandemic has not yet grasped epic proportion, but the estimation of CFR is still needed. We compared the CFR observed in Italy with the age profiles in 46 Africa countries and 2 territories which are already confirmed COVID-19 case. The estimation of the CFR in Africa ranges between (1.0%-5.4%) while in Italy is 10.1%. The five highest CFR countries and territories in Africa are Reunion (5.4%), Mauritius (5.1 %), Tunisia (3.9%), Seychelles (3.8%) and Morocco (3.3%). The last three countries with low CFR are Uganda (1.0%), Zambia (1.1%) and Angola (1.1%). The observed difference is related to the age profiles.

Research paper thumbnail of Methylation in Mycobacterium-host interaction and implications for novel control measures

Infection, Genetics and Evolution, 2020

Methylation epigenetically regulates many pivotal biological processes. Mycobacterium tuberculosi... more Methylation epigenetically regulates many pivotal biological processes. Mycobacterium tuberculosis, the pathogen of tuberculosis, can modulate host methylome. The methylated genes, sites, signaling pathway, chromatin remodeling, especially the immune related genes such as cytokines and chemokines, drug resistance and vaccines efficacy relevant genes were summarized in this paper. The results showed that methylation plays important roles in immune evasion, pathogenesis, persistence, disease progression, active, drug responder and non-responder. This will inform better practice for the development of new drugs and vaccines to eradicate tuberculosis.

Research paper thumbnail of Influence of Extract Derived In-Vitro Cell Suspension Cultures of Echinacea purpurea Against Some Immunosuppressive Effects

Research Journal of Pharmaceutical, Biological and Chemical Sciences

The present study investigates the therapeutic properties of in vitro E .purpurea cell suspension... more The present study investigates the therapeutic properties of in vitro E .purpurea cell suspension cultures against immunosuppressive effects of cyclophosphamide. Rats were assigned to four equal groups: normal control group, immune-suppressed groups: 2, 3 and 4 injected with cyclophosamide (50 mg/kg /day) i.m. for 3 consecutive days; group 2 kept as control positive, and the other two groups (3 & 4) were administered E.purpurea extract at doses 100 mg and 200 mg/kg, p.o., respectively & daily for 21 successive days. Blood samples were withdrawn twice; at the day 11and the 21 for blood cells count and the second for determination of cytokines level, γ-globulin, and MTT-value. Cyclophosphamide treated group showed significant decrease in total leucocytic count, anemia, and thrombocytopenia as well as humoral and cellular immunity markers; γ-globulin and MTT. E.purpurea treatment normalized the reduced blood cell elements in dose dependent manner. The extract inhibited production of TN...

Research paper thumbnail of Finite Groups with Quasinormal Subgroups of Prime Power Order

Acta Mathematica Hungarica, 2000

Let G be a finite group. A subgroup H is quasinormal in G if H permutes with every subgroup of G.... more Let G be a finite group. A subgroup H is quasinormal in G if H permutes with every subgroup of G. We examine the structure of G when certain abelian subgroups of prime power order are quasinormal in G.

Research paper thumbnail of On Generalized Hypercenter of a Finite Group

Communications in Algebra, 2001

Let G be a finite group. In this paper, we introduce the concept of super generalized supersolvab... more Let G be a finite group. In this paper, we introduce the concept of super generalized supersolvably embedded subgroup of a group G and give a new characterization of the generalized hypercenter of G .