Dr. Popat Mohite - Academia.edu (original) (raw)
Papers by Dr. Popat Mohite
This a preprint and has not been peer reviewed. Data may be preliminary.
IJCRT - International Journal of Creative Research Thoughts (IJCRT), Mar 1, 2021
A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liqu... more A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Abacavir (ABAC) and Lamivudine (LAMI) in bulk and tablet dosage forms. Methods: The quantification was carried out using Symmetry Premsil C18 (250 mm × 4.6 mm, 5 μm) column run in isocratic way using mobile phase comprising methanol: water (0.05% orthophosphoric acid with pH 3) 83:17 v/v and a detection wavelength of 245 nm and injection volume of 20 μl, with a flow rate of 1 ml/min. Results: In the developed method, the retention times of ABAC and LAMI were found to be 3.5 min and 7.4 min, respectively. The method was validated in terms of linearity, precision, accuracy, limits of detection, limits of quantitation, and robustness in accordance with the International Conference on Harmonization guidelines. Conclusion: The assay of the proposed method was found to be 99%-101%. The recovery studies were also carried out and mean % recovery was found to be 99%-101%. The % relative standard deviation from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of ABAC and LAMI in bulk and in tablet dosage form.
Hydrogels are three-dimensional, hydrophilic, polymeric networks capable of absorbing large amoun... more Hydrogels are three-dimensional, hydrophilic, polymeric networks capable of absorbing large amounts of water or biological fluids. Due to their high water content, porosity and soft consistency, they closely simulate natural living tissue, more so than any other class of synthetic biomaterials. Skin is the largest organ of human body and drug delivery through this route is called transdermal drug delivery system. This route of drug administration is used for local as well assystemic delivery of drug. In this review article an attempt has been made to explain the advantages, disadvantages, classification of hydrogels, methods of preparation and applications and future challenges in hydrogel based drug delivery system
Eurasian Journal of Analytical Chemistry, 2015
Combined dose tablet formulation containing Paracetamol, Caffeine and Carisoprodol is used for th... more Combined dose tablet formulation containing Paracetamol, Caffeine and Carisoprodol is used for the treatment of low back pain, post traumatic muscle spasm, sprains, strains and tenosynovitis. In this study a simple, specific, precise and accurate reverse phase high performance liquid chromatographic (RPHPLC) methods has been developed for simultaneous estimation of paracetamol (PAR), caffeine (CAF) and carisoprodol (CAR) in tablet dosage form. In the proposed chromatographic method separation was achieved by HiQ silC-18HS column (250 mm× 4.6 mm), with mobile phase containing Acetonitrile: Buffer (0.1mol L -1 Orthophosphoric Acid) (30:70 v/v) and the pH of the Buffer was adjusted to 3.1 by triethylamine. The flow rate was 1.0 ml min -1 and effluent was monitored at 232.2 nm. The retention time of CAF, PAR and CAR were 2.804 min, 4.815 min and 6.718 min respectively. The linearity for PAR, CAF and CAR were in the range of 5-25 µg mL -1 , 5-25 µg mL -1 and 10-50 µg mL -1 respectively. ...
Different tetrazole derivatives containing isoxazole has been synthesized. 5-phenyl tetrazoles (1... more Different tetrazole derivatives containing isoxazole has been synthesized. 5-phenyl tetrazoles (1) was cyclized using sodium azide and ammonium chloride and benzonitrile. The 5-phenyl tetrazoles on treatment with acetic anhydride forms 5-phenyl 1-acetyl Tetrazole (2) which on reaction with different aromatic aldehydes forms chalcones (3ah).The chalcones further undergo cyclisation with hydroxylamine hydrochloride in presence of KOH to form 5-phenyl-1-(5-substituted phenyl isoxazol-3-yl)-1H-tetrazole (4a-h).The chemical structures were confirmed by means of FT-IR, H-NMR and elemental analysis. Among the synthesized tetrazole derivatives, eight compounds have been selected and evaluated for their anticancer activity at the National Cancer Institute for testing against a panel of approximately 60 different human tumor cell lines derived from nine neoplastic cancer types. Relations between structure and activity are discussed, the most efficient anticancer compound (4b) was found to be ...
A simple, accurate and reproducible spectrophotometric method has been developed for the estimati... more A simple, accurate and reproducible spectrophotometric method has been developed for the estimation of Tranexamic acid in bulk and pharmaceutical dosage formulations. The developed method is based on the formation of condensation product of drug with ferric chloride and potassium ferricynide, which shows absorbance maximum at 710 nm. The proposed method showed linearity in the range of 2–20 ug/ml. Results were statistically validated and were found to be reproducible. Recovery studies indicate that the method is accurate.
In attempt to find new pharmacologically active molecules, we report here the synthesis and in vi... more In attempt to find new pharmacologically active molecules, we report here the synthesis and in vitro anti-inflammatory activity of various 1-[5-(substituted phenyl)-1-phenyl-4,5dihydro-1H-pyrazol-3-yl]-5-phenyl-1H-tetrazole (IV a-h).The anti-inflammatory activity of title compounds were examined by denaturation of proteins method. All the compounds exhibited weak to potent anti-inflammatory activity. Some derivatives bearing a methoxy group exhibited very good anti-inflammatory activity.
Journal of Traditional and Complementary Medicine, 2021
Background and Aim Despite advances in modern medicine, the development and growth of calculi con... more Background and Aim Despite advances in modern medicine, the development and growth of calculi continues to be a source of concern for mankind, as there is no effective treatment for kidney stones. In the present study we investigated antiurolithiatic activity of Bryophyllum pinnatum Lam against sodium oxalate (NaOx) induced urolithiasis in rats. Experimental procedure In rats with renal calculi caused by sodium oxalate (NaOx, 70 mg/kg, i.p.); the antiurolithiatic action of Bryophyllum pinnatum hydroalcoholic extract (BPHE) was studied. BPHE was given every day orally at doses of 50, 200 mg/kg for 14 days to rats to examine activity against sodium oxalate (NaOx) mediated urolithiasis, with Cystone (500 mg/kg, p.o.) as a reference standard. The effect of the extract on urine oxalate, creatinine and phosphate retention and excretion in the kidney, as well as serum and biochemical analysis of kidney homogenate and histopathological examinations were studied. Results and conclusion Oral administration of BPHE at doses of 50,100, and 200 mg/kg to rats with sodium oxalate-mediated renal calculi showed dose-dependent substantial (P<0.05) antiurolithiatic potential, with notable reversal of NaOx-induced ion excretion and urinary CaOx concentration. These findings justify the traditional use of Bryophyllum pinnatum hydroalcoholic extract (BPHE) in the treatment of renal calculi.
Iranian Journal of Analytical Chemistry, Apr 15, 2015
Eurasian Journal of …, 2010
Advanced pharmaceutical bulletin, 2012
We report here the antimycobacterial activity of some already synthesized tetrazole derivatives c... more We report here the antimycobacterial activity of some already synthesized tetrazole derivatives containing tetrazole against Mycobacterium tuberculosis strain H37Rv. In vitro evaluation of the antitubercular activity was carried out within the Tuberculosis Antimicrobial Acquisition & Coordinating Facility (TAACF) screening program for the discovery of novel drugs for the treatment of tuberculosis. Under the direction of the US National Institute of Allergy and Infectious Diseases (NIAID), Southern Research Institute that coordinates the overall program. The method of TAACF was followed for evaluation of activity. This new structural class of compounds showed high activity against the bacilli. The activity depends on the substituent's present in azatidinone core. Compounds having a 4-MeOC6H4 4-N(CH3)2C6H4 group as the substituent on β-lactam ring were active. The highest activity was registered for compounds having 4-MeOC6H4 as substituent. The new compounds showed high potency a...
A new class of Schiff bases of 4-(3-methyl-1H-1,2,4-triazol-5-yl) pyridine was synthesized to mee... more A new class of Schiff bases of 4-(3-methyl-1H-1,2,4-triazol-5-yl) pyridine was synthesized to meet structural requirements essential for the antimicrobial activity. 4-(3-methyl-1H-1,2,4-triazol-5-yl)pyridine was reacted with ethyl chloroacetate to form ethyl [3-methyl-5-(pyridin-4-yl)-1H-1,2,4-triazol-1-yl] acetate (2). Compound 2 was treated with hydrazine hydrate in methanol to yield 2-[3-methyl-5-(pyridin-4-yl)-1H-1,2,4-triazol-1-yl] acetohydrazide (3). The condensation of compound 3 with various aromatic aldehydes yielded the corresponding substituted N'-[arylidene]-2-(3-methyl-5-pyridin-4-yl-1H-1,2,4-triazol-1-yl) acetohydrazide (4a-j). The structural assessment of the newly synthesized Schiff bases (4a-j) was carried out by FT-IR, 1 H-NMR, Mass spectra and elemental analysis. The target compounds 4a-j were screened for their in vitro antimicrobial activity and the Minimum Inhibitory Concentration of each compound was determined by liquid broth method. From newly synthesize...
Pregabalin (PRG) is a new antiepileptic drug and Aceclofenac (ACE) is a potent non-steroidal anti... more Pregabalin (PRG) is a new antiepileptic drug and Aceclofenac (ACE) is a potent non-steroidal anti-inflammatory drug. These drugs in combination are used for treatment of partial seizures and neuropathic pains. A simple and precise assay method by RP-HPLC was developed and validated for estimation of PRG and ACE in Acenac-N tablet. Analyses of commercial tablet, Acenac-N were performed using JASCO Isocratic HPLC system at 248 nm on a reverse phase column HiQ sil C18HS (4.6 × 250 mm, 5μm), a mobile phase; methanol: phosphate buffer (70:30 v/v, PH 3). The validation aspects were selectivity, linearity, precision, accuracy and quantification limit. Linearity, 5-25 μg/mL for PRG and ACE respectively, provided determination coefficients (R 2 ) of 0.998 and 0.994 respectively, for PRG and ACE and proved precise since the RSD% was less than 2%. The recoveries obtained ranged from 99.10% to 100.90% for both of the drugs with RSD% less than 2%. The LOD for PRG and ACE was found to be 0.270 μg...
Orbital: The Electronic Journal of Chemistry, 2011
Some new 3-chloro-4-(substituted phenyl)-1-{[2-oxo-2-(5-phenyl-1Htetrazol1-yl) ethyl] amino} azet... more Some new 3-chloro-4-(substituted phenyl)-1-{[2-oxo-2-(5-phenyl-1Htetrazol1-yl) ethyl] amino} azetidin-2-one have been synthesized from Schiff bases of 5-phenyltetrazole. The Schiff bases were obtained by condensation 2-(5-phenyl-1H-tetrazol-1-yl) acetohydrazide with various aromatic aldehydes. Cyclocondensation of Schiff’s bases with chloroacetylchloride in the presence of triethylamine results in azetidinone derivatives. The structures of the newly synthesized azatidinones were confirmed by FT-IR, 1H NMR, 13C NMR and mass spectral data. All the synthesized compounds have exhibited significant activity against the bacteria and fungi tested.
Two simple, precise and economical spectrophotometricmethods have been developed for the estimati... more Two simple, precise and economical spectrophotometricmethods have been developed for the estimation of Ofloxacin in bulk and pharmaceutical formulations. Ofloxacin shows a sharp peak at 302.0 nmin first order derivative spectrum with n =1(MethodA). Ofloxacin also shows a zero absorbance at wavelength 320.0 nm, which is, based on zero crossingmethod (Method B). The drug follows Beer-LambertÂÂs lawin the concentration range of 2-20iÂg/ ml in both the methods. Results of the analysis were validated statistically and are found satisfactory.
In this book, the dry emulsion of Olmesartan medoxomil is prepared by spray drying method to impr... more In this book, the dry emulsion of Olmesartan medoxomil is prepared by spray drying method to improve the dissolution of poorly water soluble drug. Olmesartan medoxomil is a poorly soluble drug useful in the treatment of hypertension, absorption window of drug is stomach and upper part of small intestine.Dry emulsions can be prepared by spray drying, lyophilization and rotary evaporation. Dry emulsion was prepared by using castor oil in which drug is highly soluble, poloxamer188 as water soluble carrier and aerosil 200 as an adsorbent. The preferred oil was castor oil, olive oil, soybean oil and isopropyle myristate and polymers was PEG400, Eudragit EPO and Poloxamer 188. Stable milky formulation was formed by using surfactant (Tween 80 and span 80). Dry emulsion was evaluated for drug content, percentage moisture content, solubility. They were characterized by particle size determination, Differential Scanning Calorimetry (DSC), Powder X-ray Diffractometry (PXRD) and Scanning Electr...
methyl-2,4-dihydro-3H-pyrazol-3-one and 5-phenyl-1H-tetrazole moieties fused with substituted ary... more methyl-2,4-dihydro-3H-pyrazol-3-one and 5-phenyl-1H-tetrazole moieties fused with substituted aryl 1, 2, 4-triazoles in a single molecular frame work were designed and synthesized to meet structural requirements essential for analgesic properties. 5-methyl-2-((5-substituted aryl-4H-1, 2, 4-triazol-3-yl)methyl)-2,4- dihydro-3H-pyrazol-3-one (4a-g) and 5-phenyl-1-((5-substituted aryl-4H-1, 2, 4-triazol-3-y l)methyl)-1H-tetrazole(8a-g) derivatives were synthesized. The chemical structures of target compounds were confirmed by FT-IR, H 1 -NMR, Mass spectra and elemental analysis. The target copmpunds 4a-g and 8a-g were screened for analgesic activity by acetic acid induced writhing method and hot plate method. Functional groups like -Cl, -OCH 3 and -NO 2 containing derivatives found to be promising analgesic compounds from newly synthesized series. Synthesis of ethyl (3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl) ac- etate (2) Mixture of 5-methyl-2, 4-dihydro-3H-pyrazol-3-one (0.05 mol) i...
This a preprint and has not been peer reviewed. Data may be preliminary.
IJCRT - International Journal of Creative Research Thoughts (IJCRT), Mar 1, 2021
A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liqu... more A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Abacavir (ABAC) and Lamivudine (LAMI) in bulk and tablet dosage forms. Methods: The quantification was carried out using Symmetry Premsil C18 (250 mm × 4.6 mm, 5 μm) column run in isocratic way using mobile phase comprising methanol: water (0.05% orthophosphoric acid with pH 3) 83:17 v/v and a detection wavelength of 245 nm and injection volume of 20 μl, with a flow rate of 1 ml/min. Results: In the developed method, the retention times of ABAC and LAMI were found to be 3.5 min and 7.4 min, respectively. The method was validated in terms of linearity, precision, accuracy, limits of detection, limits of quantitation, and robustness in accordance with the International Conference on Harmonization guidelines. Conclusion: The assay of the proposed method was found to be 99%-101%. The recovery studies were also carried out and mean % recovery was found to be 99%-101%. The % relative standard deviation from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of ABAC and LAMI in bulk and in tablet dosage form.
Hydrogels are three-dimensional, hydrophilic, polymeric networks capable of absorbing large amoun... more Hydrogels are three-dimensional, hydrophilic, polymeric networks capable of absorbing large amounts of water or biological fluids. Due to their high water content, porosity and soft consistency, they closely simulate natural living tissue, more so than any other class of synthetic biomaterials. Skin is the largest organ of human body and drug delivery through this route is called transdermal drug delivery system. This route of drug administration is used for local as well assystemic delivery of drug. In this review article an attempt has been made to explain the advantages, disadvantages, classification of hydrogels, methods of preparation and applications and future challenges in hydrogel based drug delivery system
Eurasian Journal of Analytical Chemistry, 2015
Combined dose tablet formulation containing Paracetamol, Caffeine and Carisoprodol is used for th... more Combined dose tablet formulation containing Paracetamol, Caffeine and Carisoprodol is used for the treatment of low back pain, post traumatic muscle spasm, sprains, strains and tenosynovitis. In this study a simple, specific, precise and accurate reverse phase high performance liquid chromatographic (RPHPLC) methods has been developed for simultaneous estimation of paracetamol (PAR), caffeine (CAF) and carisoprodol (CAR) in tablet dosage form. In the proposed chromatographic method separation was achieved by HiQ silC-18HS column (250 mm× 4.6 mm), with mobile phase containing Acetonitrile: Buffer (0.1mol L -1 Orthophosphoric Acid) (30:70 v/v) and the pH of the Buffer was adjusted to 3.1 by triethylamine. The flow rate was 1.0 ml min -1 and effluent was monitored at 232.2 nm. The retention time of CAF, PAR and CAR were 2.804 min, 4.815 min and 6.718 min respectively. The linearity for PAR, CAF and CAR were in the range of 5-25 µg mL -1 , 5-25 µg mL -1 and 10-50 µg mL -1 respectively. ...
Different tetrazole derivatives containing isoxazole has been synthesized. 5-phenyl tetrazoles (1... more Different tetrazole derivatives containing isoxazole has been synthesized. 5-phenyl tetrazoles (1) was cyclized using sodium azide and ammonium chloride and benzonitrile. The 5-phenyl tetrazoles on treatment with acetic anhydride forms 5-phenyl 1-acetyl Tetrazole (2) which on reaction with different aromatic aldehydes forms chalcones (3ah).The chalcones further undergo cyclisation with hydroxylamine hydrochloride in presence of KOH to form 5-phenyl-1-(5-substituted phenyl isoxazol-3-yl)-1H-tetrazole (4a-h).The chemical structures were confirmed by means of FT-IR, H-NMR and elemental analysis. Among the synthesized tetrazole derivatives, eight compounds have been selected and evaluated for their anticancer activity at the National Cancer Institute for testing against a panel of approximately 60 different human tumor cell lines derived from nine neoplastic cancer types. Relations between structure and activity are discussed, the most efficient anticancer compound (4b) was found to be ...
A simple, accurate and reproducible spectrophotometric method has been developed for the estimati... more A simple, accurate and reproducible spectrophotometric method has been developed for the estimation of Tranexamic acid in bulk and pharmaceutical dosage formulations. The developed method is based on the formation of condensation product of drug with ferric chloride and potassium ferricynide, which shows absorbance maximum at 710 nm. The proposed method showed linearity in the range of 2–20 ug/ml. Results were statistically validated and were found to be reproducible. Recovery studies indicate that the method is accurate.
In attempt to find new pharmacologically active molecules, we report here the synthesis and in vi... more In attempt to find new pharmacologically active molecules, we report here the synthesis and in vitro anti-inflammatory activity of various 1-[5-(substituted phenyl)-1-phenyl-4,5dihydro-1H-pyrazol-3-yl]-5-phenyl-1H-tetrazole (IV a-h).The anti-inflammatory activity of title compounds were examined by denaturation of proteins method. All the compounds exhibited weak to potent anti-inflammatory activity. Some derivatives bearing a methoxy group exhibited very good anti-inflammatory activity.
Journal of Traditional and Complementary Medicine, 2021
Background and Aim Despite advances in modern medicine, the development and growth of calculi con... more Background and Aim Despite advances in modern medicine, the development and growth of calculi continues to be a source of concern for mankind, as there is no effective treatment for kidney stones. In the present study we investigated antiurolithiatic activity of Bryophyllum pinnatum Lam against sodium oxalate (NaOx) induced urolithiasis in rats. Experimental procedure In rats with renal calculi caused by sodium oxalate (NaOx, 70 mg/kg, i.p.); the antiurolithiatic action of Bryophyllum pinnatum hydroalcoholic extract (BPHE) was studied. BPHE was given every day orally at doses of 50, 200 mg/kg for 14 days to rats to examine activity against sodium oxalate (NaOx) mediated urolithiasis, with Cystone (500 mg/kg, p.o.) as a reference standard. The effect of the extract on urine oxalate, creatinine and phosphate retention and excretion in the kidney, as well as serum and biochemical analysis of kidney homogenate and histopathological examinations were studied. Results and conclusion Oral administration of BPHE at doses of 50,100, and 200 mg/kg to rats with sodium oxalate-mediated renal calculi showed dose-dependent substantial (P<0.05) antiurolithiatic potential, with notable reversal of NaOx-induced ion excretion and urinary CaOx concentration. These findings justify the traditional use of Bryophyllum pinnatum hydroalcoholic extract (BPHE) in the treatment of renal calculi.
Iranian Journal of Analytical Chemistry, Apr 15, 2015
Eurasian Journal of …, 2010
Advanced pharmaceutical bulletin, 2012
We report here the antimycobacterial activity of some already synthesized tetrazole derivatives c... more We report here the antimycobacterial activity of some already synthesized tetrazole derivatives containing tetrazole against Mycobacterium tuberculosis strain H37Rv. In vitro evaluation of the antitubercular activity was carried out within the Tuberculosis Antimicrobial Acquisition & Coordinating Facility (TAACF) screening program for the discovery of novel drugs for the treatment of tuberculosis. Under the direction of the US National Institute of Allergy and Infectious Diseases (NIAID), Southern Research Institute that coordinates the overall program. The method of TAACF was followed for evaluation of activity. This new structural class of compounds showed high activity against the bacilli. The activity depends on the substituent's present in azatidinone core. Compounds having a 4-MeOC6H4 4-N(CH3)2C6H4 group as the substituent on β-lactam ring were active. The highest activity was registered for compounds having 4-MeOC6H4 as substituent. The new compounds showed high potency a...
A new class of Schiff bases of 4-(3-methyl-1H-1,2,4-triazol-5-yl) pyridine was synthesized to mee... more A new class of Schiff bases of 4-(3-methyl-1H-1,2,4-triazol-5-yl) pyridine was synthesized to meet structural requirements essential for the antimicrobial activity. 4-(3-methyl-1H-1,2,4-triazol-5-yl)pyridine was reacted with ethyl chloroacetate to form ethyl [3-methyl-5-(pyridin-4-yl)-1H-1,2,4-triazol-1-yl] acetate (2). Compound 2 was treated with hydrazine hydrate in methanol to yield 2-[3-methyl-5-(pyridin-4-yl)-1H-1,2,4-triazol-1-yl] acetohydrazide (3). The condensation of compound 3 with various aromatic aldehydes yielded the corresponding substituted N'-[arylidene]-2-(3-methyl-5-pyridin-4-yl-1H-1,2,4-triazol-1-yl) acetohydrazide (4a-j). The structural assessment of the newly synthesized Schiff bases (4a-j) was carried out by FT-IR, 1 H-NMR, Mass spectra and elemental analysis. The target compounds 4a-j were screened for their in vitro antimicrobial activity and the Minimum Inhibitory Concentration of each compound was determined by liquid broth method. From newly synthesize...
Pregabalin (PRG) is a new antiepileptic drug and Aceclofenac (ACE) is a potent non-steroidal anti... more Pregabalin (PRG) is a new antiepileptic drug and Aceclofenac (ACE) is a potent non-steroidal anti-inflammatory drug. These drugs in combination are used for treatment of partial seizures and neuropathic pains. A simple and precise assay method by RP-HPLC was developed and validated for estimation of PRG and ACE in Acenac-N tablet. Analyses of commercial tablet, Acenac-N were performed using JASCO Isocratic HPLC system at 248 nm on a reverse phase column HiQ sil C18HS (4.6 × 250 mm, 5μm), a mobile phase; methanol: phosphate buffer (70:30 v/v, PH 3). The validation aspects were selectivity, linearity, precision, accuracy and quantification limit. Linearity, 5-25 μg/mL for PRG and ACE respectively, provided determination coefficients (R 2 ) of 0.998 and 0.994 respectively, for PRG and ACE and proved precise since the RSD% was less than 2%. The recoveries obtained ranged from 99.10% to 100.90% for both of the drugs with RSD% less than 2%. The LOD for PRG and ACE was found to be 0.270 μg...
Orbital: The Electronic Journal of Chemistry, 2011
Some new 3-chloro-4-(substituted phenyl)-1-{[2-oxo-2-(5-phenyl-1Htetrazol1-yl) ethyl] amino} azet... more Some new 3-chloro-4-(substituted phenyl)-1-{[2-oxo-2-(5-phenyl-1Htetrazol1-yl) ethyl] amino} azetidin-2-one have been synthesized from Schiff bases of 5-phenyltetrazole. The Schiff bases were obtained by condensation 2-(5-phenyl-1H-tetrazol-1-yl) acetohydrazide with various aromatic aldehydes. Cyclocondensation of Schiff’s bases with chloroacetylchloride in the presence of triethylamine results in azetidinone derivatives. The structures of the newly synthesized azatidinones were confirmed by FT-IR, 1H NMR, 13C NMR and mass spectral data. All the synthesized compounds have exhibited significant activity against the bacteria and fungi tested.
Two simple, precise and economical spectrophotometricmethods have been developed for the estimati... more Two simple, precise and economical spectrophotometricmethods have been developed for the estimation of Ofloxacin in bulk and pharmaceutical formulations. Ofloxacin shows a sharp peak at 302.0 nmin first order derivative spectrum with n =1(MethodA). Ofloxacin also shows a zero absorbance at wavelength 320.0 nm, which is, based on zero crossingmethod (Method B). The drug follows Beer-LambertÂÂs lawin the concentration range of 2-20iÂg/ ml in both the methods. Results of the analysis were validated statistically and are found satisfactory.
In this book, the dry emulsion of Olmesartan medoxomil is prepared by spray drying method to impr... more In this book, the dry emulsion of Olmesartan medoxomil is prepared by spray drying method to improve the dissolution of poorly water soluble drug. Olmesartan medoxomil is a poorly soluble drug useful in the treatment of hypertension, absorption window of drug is stomach and upper part of small intestine.Dry emulsions can be prepared by spray drying, lyophilization and rotary evaporation. Dry emulsion was prepared by using castor oil in which drug is highly soluble, poloxamer188 as water soluble carrier and aerosil 200 as an adsorbent. The preferred oil was castor oil, olive oil, soybean oil and isopropyle myristate and polymers was PEG400, Eudragit EPO and Poloxamer 188. Stable milky formulation was formed by using surfactant (Tween 80 and span 80). Dry emulsion was evaluated for drug content, percentage moisture content, solubility. They were characterized by particle size determination, Differential Scanning Calorimetry (DSC), Powder X-ray Diffractometry (PXRD) and Scanning Electr...
methyl-2,4-dihydro-3H-pyrazol-3-one and 5-phenyl-1H-tetrazole moieties fused with substituted ary... more methyl-2,4-dihydro-3H-pyrazol-3-one and 5-phenyl-1H-tetrazole moieties fused with substituted aryl 1, 2, 4-triazoles in a single molecular frame work were designed and synthesized to meet structural requirements essential for analgesic properties. 5-methyl-2-((5-substituted aryl-4H-1, 2, 4-triazol-3-yl)methyl)-2,4- dihydro-3H-pyrazol-3-one (4a-g) and 5-phenyl-1-((5-substituted aryl-4H-1, 2, 4-triazol-3-y l)methyl)-1H-tetrazole(8a-g) derivatives were synthesized. The chemical structures of target compounds were confirmed by FT-IR, H 1 -NMR, Mass spectra and elemental analysis. The target copmpunds 4a-g and 8a-g were screened for analgesic activity by acetic acid induced writhing method and hot plate method. Functional groups like -Cl, -OCH 3 and -NO 2 containing derivatives found to be promising analgesic compounds from newly synthesized series. Synthesis of ethyl (3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl) ac- etate (2) Mixture of 5-methyl-2, 4-dihydro-3H-pyrazol-3-one (0.05 mol) i...