Monica Deming - Academia.edu (original) (raw)

Papers by Monica Deming

Archives For Dermatological Research, Apr 8, 2009

Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at... more Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.

Investigative Radiology, 2009

Nephrogenic systemic fibrosis (NSF) is a clinical syndrome linked with exposure in renal failure ... more Nephrogenic systemic fibrosis (NSF) is a clinical syndrome linked with exposure in renal failure patients to gadolinium-based magnetic resonance imaging contrast agents (GBCAs). The pathogenesis of the disease is largely unknown. The present study addresses potential pathophysiological mechanisms. Here, we have examined human skin in organ culture and human dermal fibroblasts in monolayer culture for responses to GBCA stimulation. Treatment of normal human skin in organ culture with Omniscan had no significant effect on type I procollagen but increased both matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1. At the histologic level, many interstitial cells demonstrated cytologic features characteristic of activation (ie, light staining, oblong, plump nuclei). Omniscan, as well as 3 other magnetic resonance imaging contrast agents (Magnevist, Multihance, and Prohance), increased proliferation of human dermal fibroblasts in monolayer culture. Increased proliferation was accompanied by an increase in production of both matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1 but no increase in type I procollagen. Concentrations required for effects differed among the 4 agents (Omniscan < Magnevist and Multihance < Prohance). In contrast to its effects on fibroblast function, Omniscan did not stimulate human epidermal keratinocyte proliferation when examined over a wide range of concentrations. These data provide evidence that GBCA exposure in ex vivo skin from healthy individuals increases fibroblast proliferation and has effects on the enzyme/inhibitor system that regulates collagen turnover in the skin.

Investigative Radiology, 2010

Objective-Nephrogenic systemic fibrosis (NSF) is a clinical syndrome occurring in a small subset ... more Objective-Nephrogenic systemic fibrosis (NSF) is a clinical syndrome occurring in a small subset of patients with end stage renal disease (ESRD). Exposure to certain of the gadoliniumbased contrast agents (GBCAs) during magnetic resonance imaging appears to be a trigger. The pathogenesis of the disease is largely unknown. The present study addresses potential pathophysiological mechanisms.

Archives of Dermatological Research, 2009

Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at... more Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.

In states where climate change is often portrayed as unsound or unsettled science, teaching about... more In states where climate change is often portrayed as unsound or unsettled science, teaching about weather, climate, and climate change in the classroom can be dicey. For the past two years, the Oklahoma Climatological Survey in conjunction with Oklahomans for Excellence in Science Education has hosted a 3-day workshop at the University of Oklahoma Biostation at Lake Texoma in southern Oklahoma to address this problem. This workshop is designed to make Oklahoma and Texas K-12 teachers more knowledgeable about weather and climate in general and to help them navigate the tricky subject of teaching climate change in a classroom. The current political climate in both Oklahoma and Texas was one of the driving factors in the establishment of this atmospheric climate workshop. Over the past two years, more than 60 Oklahoma scientists and educators collaborated to revamp the state standards for science to replace the old Oklahoma Priority Academic Student Skills for Science (PASS) that many ...

Archives For Dermatological Research, Apr 8, 2009

Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at... more Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.

Investigative Radiology, 2009

Nephrogenic systemic fibrosis (NSF) is a clinical syndrome linked with exposure in renal failure ... more Nephrogenic systemic fibrosis (NSF) is a clinical syndrome linked with exposure in renal failure patients to gadolinium-based magnetic resonance imaging contrast agents (GBCAs). The pathogenesis of the disease is largely unknown. The present study addresses potential pathophysiological mechanisms. Here, we have examined human skin in organ culture and human dermal fibroblasts in monolayer culture for responses to GBCA stimulation. Treatment of normal human skin in organ culture with Omniscan had no significant effect on type I procollagen but increased both matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1. At the histologic level, many interstitial cells demonstrated cytologic features characteristic of activation (ie, light staining, oblong, plump nuclei). Omniscan, as well as 3 other magnetic resonance imaging contrast agents (Magnevist, Multihance, and Prohance), increased proliferation of human dermal fibroblasts in monolayer culture. Increased proliferation was accompanied by an increase in production of both matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1 but no increase in type I procollagen. Concentrations required for effects differed among the 4 agents (Omniscan < Magnevist and Multihance < Prohance). In contrast to its effects on fibroblast function, Omniscan did not stimulate human epidermal keratinocyte proliferation when examined over a wide range of concentrations. These data provide evidence that GBCA exposure in ex vivo skin from healthy individuals increases fibroblast proliferation and has effects on the enzyme/inhibitor system that regulates collagen turnover in the skin.

Investigative Radiology, 2010

Objective-Nephrogenic systemic fibrosis (NSF) is a clinical syndrome occurring in a small subset ... more Objective-Nephrogenic systemic fibrosis (NSF) is a clinical syndrome occurring in a small subset of patients with end stage renal disease (ESRD). Exposure to certain of the gadoliniumbased contrast agents (GBCAs) during magnetic resonance imaging appears to be a trigger. The pathogenesis of the disease is largely unknown. The present study addresses potential pathophysiological mechanisms.

Archives of Dermatological Research, 2009

Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at... more Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.

In states where climate change is often portrayed as unsound or unsettled science, teaching about... more In states where climate change is often portrayed as unsound or unsettled science, teaching about weather, climate, and climate change in the classroom can be dicey. For the past two years, the Oklahoma Climatological Survey in conjunction with Oklahomans for Excellence in Science Education has hosted a 3-day workshop at the University of Oklahoma Biostation at Lake Texoma in southern Oklahoma to address this problem. This workshop is designed to make Oklahoma and Texas K-12 teachers more knowledgeable about weather and climate in general and to help them navigate the tricky subject of teaching climate change in a classroom. The current political climate in both Oklahoma and Texas was one of the driving factors in the establishment of this atmospheric climate workshop. Over the past two years, more than 60 Oklahoma scientists and educators collaborated to revamp the state standards for science to replace the old Oklahoma Priority Academic Student Skills for Science (PASS) that many ...