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Research paper thumbnail of Two endodontics techniques analysis in primary molars with fistula

RGO - Revista Gaúcha de Odontologia, Jun 1, 2015

Objective The purpose of this research was to follow the radiolucent area (pixels) in the furcati... more Objective The purpose of this research was to follow the radiolucent area (pixels) in the furcation area and the behavior of the endodontically treated deciduous molars with different simplified echniques. Methods The treatments were performed without the duct instrumentation using relative isolation and two drugs were applied, as follows: G1: Guedes-Pinto paste (GP) and G2: CTZ paste (CTZ) for the pulp chamber filling. The radiolucent areas in the furcation region of deciduous molars were obtained from previous x-rays, after 6 and 12 months of the endodontic treatment and was analysed by l Image Tool 3.0 by two blinded examiners. In order to analyse the calibration between the examiners, Kappa test was used and for comparison of the initial radiographic image, analysis of variance was used after 6 and 12 months of each group and Tukey spost-hoc test. T-Student test was used for comparison between groups. Results There was a reduction of radiolucent area after 6 (p<0.01) and 12 months (p<0.01) in the teeth treated with CTZ paste. In the teeth treated with PG there was not reduction of radiolucent area after 12 months. (p= 0,09). Conclusion Although the clinical signs were similar in both groups after 12 months of follow up, only teeth treated with CTZ showed reduction of radiolucent area in the furca area after 6 and 12 months.

Research paper thumbnail of Performance of fluorescence-based and conventional methods of occlusal caries detection in primary molars - anin vitrostudy

International Journal of Paediatric Dentistry, 2012

This in vitro study aimed to test the performance of fluorescence-based methods in detecting occl... more This in vitro study aimed to test the performance of fluorescence-based methods in detecting occlusal caries lesions in primary molars compared to conventional methods. Two examiners assessed 113 sites on 77 occlusal surfaces of primary molars using three fluorescence devices: DIAGNOdent (LF), DIAGNOdent pen (LFpen), and fluorescence camera (VistaProof-FC). Visual inspection (ICDAS) and radiographic methods were also evaluated. One examiner repeated the evaluations after one month. As reference standard method, the lesion depth was determined after sectioning and evaluation in stereomicroscope. The area under the ROC curve (Az), sensitivity, specificity, and accuracy of the methods were calculated at enamel (D1) and dentine caries (D3) lesions thresholds. The intra and interexaminer reproducibility were calculated using the intraclass correlation coefficient (ICC) and kappa statistics. At D1, visual inspection presented higher sensitivities (0.97-0.99) but lower specificities (0.18-0.25). At D3, all the methods demonstrated similar performance (Az values around 0.90). Visual and radiographic methods showed a slightly higher specificity (values higher than 0.96) than the fluorescence based ones (values around 0.88). In general, all methods presented high reproducibility (ICC higher than 0.79). Although fluorescence-based and conventional methods present similar performance in detecting occlusal caries lesions in primary teeth, visual inspection alone seems to be sufficient to be used in clinical practice.

Research paper thumbnail of New methodology to assess activity status of occlusal caries in primary teeth using laser fluorescence device

Journal of Biomedical Optics, 2010

An in vivo study was conducted to verify the ability of laser fluorescence ͑LF͒ to assess the act... more An in vivo study was conducted to verify the ability of laser fluorescence ͑LF͒ to assess the activity status of occlusal caries in primary teeth, using different air-drying times. Occlusal sites ͑707͒ were examined using LF ͑DIAGNOdent͒ after air-drying for 3 s and 15 s, and the difference between readings ͑DIF15 s-3 s͒ was calculated. For concurrent validation of LF, visual criteria-Nyvad ͑NY͒ and Lesion Activity Assessment associated with the International Caries Detection and Assessment System ͑LAA-ICDAS͒-were the reference standards for lesion activity. Histological exam using a pH-indicator dye ͑0.1% methyl red͒ was performed in 46 exfoliated/extracted teeth for criterion validation. LF readings and DIF15 s-3 s were compared using Kruskall-Wallis and Mann-Whitney tests. Receiver operating characteristic analyses were performed and validity parameters calculated, considering the caries activity assessment. Using NY, active lesions ͑3 s: 30.0± 29.3; 15 s: 34.2± 30.6͒ presented higher LF readings than inactive lesions ͑3 s: 17.0± 16.3; 15 s: 19.2± 17.3; p Ͻ 0.05͒, different from LAA-ICDAS. Active cavitated caries resulted in higher LF readings ͑3 s: 50.3± 3.5; 15 s: 54.7± 30.2͒ than inactive cavitated caries ͑3 s: 19.9± 16.3; 15 s: 22.8± 16.8͒. Therefore, LF can distinguish cavitated active and inactive lesions classified by NY, but not by LAA-ICDAS; however, this difference might be related to the visual system rather than to LF. The air-drying time could be an alternative to improve the caries activity assessment; however, longer air-drying time is suggested to be tested subsequently.

Research paper thumbnail of Tratamento Pulpar Indireto em Molares Decíduos em Sessão Única: Estudo Retrospectivo

… e Clínica Integrada, 2010

Research paper thumbnail of Comparing the reliability of a new fluorescence camera with conventional laser fluorescence devices in detecting caries lesions in occlusal and smooth surfaces of primary teeth

Lasers in Medical Science, 2011

The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera ... more The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera (FC; VistaProof) with that of two other laser fluorescence devices (DIAGNOdent and DIAGNOdent pen) in assessing smooth and occlusal caries lesions in primary teeth and (2) to determine the influence of the spacer, the protective cover and air drying on the FC readings. We selected 129 primary molars which were divided into two groups: smooth surfaces (n=68) and occlusal surfaces (n=61). Two trained examiners performed the evaluations using the FC and the other laser fluorescence devices separately. One examiner repeated all assessments after 1 week. Inter- and intraexaminer reproducibility was calculated from the absolute values derived from the devices using intraclass correlation coefficient (ICC) and Bland-Altman analysis. Pearson correlation was performed among the values obtained with the different devices. For aim 2, only teeth from the occlusal surface group were used. Another exa...

Research paper thumbnail of Comparing the reliability of a new fluorescence camera with conventional laser fluorescence devices in detecting caries lesions in occlusal and smooth surfaces of primary teeth

Lasers in Medical Science, 2011

The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera ... more The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera (FC; VistaProof) with that of two other laser fluorescence devices (DIAGNOdent and DIAGNOdent pen) in assessing smooth and occlusal caries lesions in primary teeth and (2) to determine the influence of the spacer, the protective cover and air drying on the FC readings. We selected 129 primary molars which were divided into two groups: smooth surfaces (n=68) and occlusal surfaces (n=61). Two trained examiners performed the evaluations using the FC and the other laser fluorescence devices separately. One examiner repeated all assessments after 1 week. Inter- and intraexaminer reproducibility was calculated from the absolute values derived from the devices using intraclass correlation coefficient (ICC) and Bland-Altman analysis. Pearson correlation was performed among the values obtained with the different devices. For aim 2, only teeth from the occlusal surface group were used. Another exa...

Research paper thumbnail of Two endodontics techniques analysis in primary molars with fistula

RGO - Revista Gaúcha de Odontologia, Jun 1, 2015

Objective The purpose of this research was to follow the radiolucent area (pixels) in the furcati... more Objective The purpose of this research was to follow the radiolucent area (pixels) in the furcation area and the behavior of the endodontically treated deciduous molars with different simplified echniques. Methods The treatments were performed without the duct instrumentation using relative isolation and two drugs were applied, as follows: G1: Guedes-Pinto paste (GP) and G2: CTZ paste (CTZ) for the pulp chamber filling. The radiolucent areas in the furcation region of deciduous molars were obtained from previous x-rays, after 6 and 12 months of the endodontic treatment and was analysed by l Image Tool 3.0 by two blinded examiners. In order to analyse the calibration between the examiners, Kappa test was used and for comparison of the initial radiographic image, analysis of variance was used after 6 and 12 months of each group and Tukey spost-hoc test. T-Student test was used for comparison between groups. Results There was a reduction of radiolucent area after 6 (p<0.01) and 12 months (p<0.01) in the teeth treated with CTZ paste. In the teeth treated with PG there was not reduction of radiolucent area after 12 months. (p= 0,09). Conclusion Although the clinical signs were similar in both groups after 12 months of follow up, only teeth treated with CTZ showed reduction of radiolucent area in the furca area after 6 and 12 months.

Research paper thumbnail of Performance of fluorescence-based and conventional methods of occlusal caries detection in primary molars - anin vitrostudy

International Journal of Paediatric Dentistry, 2012

This in vitro study aimed to test the performance of fluorescence-based methods in detecting occl... more This in vitro study aimed to test the performance of fluorescence-based methods in detecting occlusal caries lesions in primary molars compared to conventional methods. Two examiners assessed 113 sites on 77 occlusal surfaces of primary molars using three fluorescence devices: DIAGNOdent (LF), DIAGNOdent pen (LFpen), and fluorescence camera (VistaProof-FC). Visual inspection (ICDAS) and radiographic methods were also evaluated. One examiner repeated the evaluations after one month. As reference standard method, the lesion depth was determined after sectioning and evaluation in stereomicroscope. The area under the ROC curve (Az), sensitivity, specificity, and accuracy of the methods were calculated at enamel (D1) and dentine caries (D3) lesions thresholds. The intra and interexaminer reproducibility were calculated using the intraclass correlation coefficient (ICC) and kappa statistics. At D1, visual inspection presented higher sensitivities (0.97-0.99) but lower specificities (0.18-0.25). At D3, all the methods demonstrated similar performance (Az values around 0.90). Visual and radiographic methods showed a slightly higher specificity (values higher than 0.96) than the fluorescence based ones (values around 0.88). In general, all methods presented high reproducibility (ICC higher than 0.79). Although fluorescence-based and conventional methods present similar performance in detecting occlusal caries lesions in primary teeth, visual inspection alone seems to be sufficient to be used in clinical practice.

Research paper thumbnail of New methodology to assess activity status of occlusal caries in primary teeth using laser fluorescence device

Journal of Biomedical Optics, 2010

An in vivo study was conducted to verify the ability of laser fluorescence ͑LF͒ to assess the act... more An in vivo study was conducted to verify the ability of laser fluorescence ͑LF͒ to assess the activity status of occlusal caries in primary teeth, using different air-drying times. Occlusal sites ͑707͒ were examined using LF ͑DIAGNOdent͒ after air-drying for 3 s and 15 s, and the difference between readings ͑DIF15 s-3 s͒ was calculated. For concurrent validation of LF, visual criteria-Nyvad ͑NY͒ and Lesion Activity Assessment associated with the International Caries Detection and Assessment System ͑LAA-ICDAS͒-were the reference standards for lesion activity. Histological exam using a pH-indicator dye ͑0.1% methyl red͒ was performed in 46 exfoliated/extracted teeth for criterion validation. LF readings and DIF15 s-3 s were compared using Kruskall-Wallis and Mann-Whitney tests. Receiver operating characteristic analyses were performed and validity parameters calculated, considering the caries activity assessment. Using NY, active lesions ͑3 s: 30.0± 29.3; 15 s: 34.2± 30.6͒ presented higher LF readings than inactive lesions ͑3 s: 17.0± 16.3; 15 s: 19.2± 17.3; p Ͻ 0.05͒, different from LAA-ICDAS. Active cavitated caries resulted in higher LF readings ͑3 s: 50.3± 3.5; 15 s: 54.7± 30.2͒ than inactive cavitated caries ͑3 s: 19.9± 16.3; 15 s: 22.8± 16.8͒. Therefore, LF can distinguish cavitated active and inactive lesions classified by NY, but not by LAA-ICDAS; however, this difference might be related to the visual system rather than to LF. The air-drying time could be an alternative to improve the caries activity assessment; however, longer air-drying time is suggested to be tested subsequently.

Research paper thumbnail of Tratamento Pulpar Indireto em Molares Decíduos em Sessão Única: Estudo Retrospectivo

… e Clínica Integrada, 2010

Research paper thumbnail of Comparing the reliability of a new fluorescence camera with conventional laser fluorescence devices in detecting caries lesions in occlusal and smooth surfaces of primary teeth

Lasers in Medical Science, 2011

The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera ... more The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera (FC; VistaProof) with that of two other laser fluorescence devices (DIAGNOdent and DIAGNOdent pen) in assessing smooth and occlusal caries lesions in primary teeth and (2) to determine the influence of the spacer, the protective cover and air drying on the FC readings. We selected 129 primary molars which were divided into two groups: smooth surfaces (n=68) and occlusal surfaces (n=61). Two trained examiners performed the evaluations using the FC and the other laser fluorescence devices separately. One examiner repeated all assessments after 1 week. Inter- and intraexaminer reproducibility was calculated from the absolute values derived from the devices using intraclass correlation coefficient (ICC) and Bland-Altman analysis. Pearson correlation was performed among the values obtained with the different devices. For aim 2, only teeth from the occlusal surface group were used. Another exa...

Research paper thumbnail of Comparing the reliability of a new fluorescence camera with conventional laser fluorescence devices in detecting caries lesions in occlusal and smooth surfaces of primary teeth

Lasers in Medical Science, 2011

The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera ... more The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera (FC; VistaProof) with that of two other laser fluorescence devices (DIAGNOdent and DIAGNOdent pen) in assessing smooth and occlusal caries lesions in primary teeth and (2) to determine the influence of the spacer, the protective cover and air drying on the FC readings. We selected 129 primary molars which were divided into two groups: smooth surfaces (n=68) and occlusal surfaces (n=61). Two trained examiners performed the evaluations using the FC and the other laser fluorescence devices separately. One examiner repeated all assessments after 1 week. Inter- and intraexaminer reproducibility was calculated from the absolute values derived from the devices using intraclass correlation coefficient (ICC) and Bland-Altman analysis. Pearson correlation was performed among the values obtained with the different devices. For aim 2, only teeth from the occlusal surface group were used. Another exa...