Mrityunjoy Mahato - Academia.edu (original) (raw)

Papers by Mrityunjoy Mahato

Research paper thumbnail of Effect of Salt on the Formation of Alcohol-Dehydrogenease Monolayer: A Study by the Langmuir−Blodgett Technique

The Journal of Physical Chemistry B, 2009

We report here the effect of salt (KCl) on the interfacial surface activity of yeast alcohol dehy... more We report here the effect of salt (KCl) on the interfacial surface activity of yeast alcohol dehydogenease (ADH) at air/water interface using the Langmuir-Blodgett technique. Effect of salt content in the water subphase on ADH structure has been studied. The change of area/molecule, compressibility, rigidity, and unfolding of ADH are insignificant up to 10 mM KCl concentration. The significant changes are observed above 0.1 M KCl concentrations. Observations are explained in the context of DLVO theory. FTIR study of amide band together with AFM imaging of ADH monolayer indicate that KCl perturbs the ADH monolayer by the increment of-structure resulting into larger unfolding and intermolecular aggregates at high salt concentration.

Research paper thumbnail of Mn-doped SrTiO3 and SrTiO3–Fe2O3 composite perovskites: photocatalytic dye degradation and energy storage application

Journal of Materials Science: Materials in Electronics

Research paper thumbnail of Studies on the interaction and complexation of pyrrole compound with hemoglobin

Department of Chemistry, School of Basic and Applied Sciences, Career Point University, Hamirpur-... more Department of Chemistry, School of Basic and Applied Sciences, Career Point University, Hamirpur-176 041, Himachal Pradesh, India E-mail : sanjitmahato@gmail.com Department of Chemical Engineering, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA), South Africa Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi-835 215, Jharkhand, India Physics Division, Department of Basic Sciences and Social Sciences, School of Technology, North Eastern Hill University, Shillong-793 022, Meghalaya, India <em>E-mail</em> : mrityunjoyphy@gmail.com DST/CSIR Innovation Centre, National Centre for Nanostructured Materials, CSIR Material Science and Manufacturing, Building-19B, PO Box 395, Pretoria-0001, South Africa Currently Research Scientist at TCG Lifesciences Pvt. Ltd., Kolkata-700 091, India <em>Manuscript received 23 June 2017, accepted 15 July 2017</em> Alkaloids such as pyrrole, tetrap...

Research paper thumbnail of Biosensors for detection of prostate cancer: a review

Research paper thumbnail of Studies on protein conformation in water and air water interface by Langmuir Blodgett technique

This thesis deals with some multi disciplinary work involving the combination of different techni... more This thesis deals with some multi disciplinary work involving the combination of different techniques on the basic study of development and characterization of protein and protein based bioactive thin film at air/water interface and subsequent transfer onto solid substrate.

Research paper thumbnail of Waste plastic-derived NiO-MWCNT composite as visible light photocatalyst for degradation of methylene blue dye

Nanotechnology for Environmental Engineering

Research paper thumbnail of Recent Advances on Polymer Nanocomposite-Based Radiation Shielding Materials for Medical Science

Nowadays, radiation is an integral part of our lives and economy by means of natural and human ac... more Nowadays, radiation is an integral part of our lives and economy by means of natural and human activity such as UV radiation (forensic analysis, medical imaging) (www.sciencing.com), microwave radiation (mobile phone, radar) (www.ffden-2.phys.uaf.edu), X-ray (radiology, security scan, material analysis) (www.labmate-online.com), and alpha, beta, gamma radiations (nuclear medicine, radiotherapy) (www.bbc.co.uk). The unwanted exposure to these radiations can lead to health disorder (cancer, birth defect) and other kinds of accidents. Therefore, protection of our health and environment from the radiation is necessary, and development of radiation protection nanocomposite is now an emergent field of research. Usually, a high-density material lead is used to protect in general from any kind of radiation and in particular from nuclear radiation. However, due to high atomic weight and high toxicity of lead, it has limited applications, and researchers are encouraged to look for a lightweig...

Research paper thumbnail of Herbal Nanocarriers for Cancer Therapy

Cancer is a group of around 100 diseases that has been tormenting mankind since ancient time. Due... more Cancer is a group of around 100 diseases that has been tormenting mankind since ancient time. Due to cancer, estimated 8.2 million people died globally in 2012, and the toll is expected to reach 13 million in 2030. Despite the improvement of conventional therapeutic modalities, the outcome of cancer patients has not improved significantly. So, alternative therapeutic modalities and new effective anticancer drugs are highly sought for. Different parts of plants and their extracts have been used to cure many diseases and relive from physical agony since ancient times. In the traditional system of medicine, herbal products have been used for treating different types of diseases and alignments globally. Active compounds from herbal medicine, such as curcumin, are found to be effective against cancer. Despite their excellent therapeutic ability, the potential of these herbal compounds or phytochemicals is limited due to their low water solubility and poor bioavailability.

Research paper thumbnail of Measurement of elastic modulus of CNT composites: a nondestructive study

Fullerenes, Nanotubes and Carbon Nanostructures

CNT based nanocomposites are known to have superior mechanical properties, such as elastic modulu... more CNT based nanocomposites are known to have superior mechanical properties, such as elastic modulus (E), tensile strength (σ), etc., compared to other nanocomposite materials. Traditionally E of the...

Research paper thumbnail of Paper-Based Sensors for Biomedical Applications

Biomedical Engineering and its Applications in Healthcare

Paper-based sensor is a new analytical technique applicable in healthcare monitoring, environment... more Paper-based sensor is a new analytical technique applicable in healthcare monitoring, environmental monitoring, food quality control, etc. Currently, these technologies are drawing great attention due to ease of fabrication, cost-effectiveness, and their simple, portable, and disposable nature. The inherent qualities of paper, such as availability in large quantities, cheap, lightweight, and biodegradability, are being exploited to develop sustainable devices. The application of paper as a substrate material in litmus paper was dated back to seventeenth century. The semiquantitative detection of glucose in urine and immune chromatographic paper test strips for pregnancy test kits are examples of paper-based sensing devices. Today, microfluidic paper-based sensors with advanced designs involving complex 3D geometrics for handling multiples analytes are under investigation. It is foreseen that advancements in the fabrication and analytical techniques will help in improving the accuracy and sensitivity of paper-based sensors. Different types of detection approaches used in microfluidic paper-based devices are colorimetry, luminescence, electrochemical detection, fluorescence, and surface-enhanced Raman scattering.

Research paper thumbnail of Metalloprotein-Inspired Ruthenium Polymeric Complex: A Highly Efficient Catalyst in Parts per Million Level for 1,3-Dipolar Huisgen’s Reaction in Aqueous Medium at Room Temperature

Industrial & Engineering Chemistry Research, 2017

Research paper thumbnail of Waste derived low cost PbO-Carbon nanocomposite and its energy storage application

Materials Today: Proceedings

Research paper thumbnail of Preparation, characterization and some electrochemical study of waste derived iron Oxide-Carbon nanocomposite

Materials Today: Proceedings

Research paper thumbnail of Conversion of Solid Waste into Functional Carbon Materials: A Review

Energy and Environment Focus

Research paper thumbnail of Biomineralized Nanocrystal of Calcium Carbonate in Protein Langmuir Blodgett Monolayer

Materials Today: Proceedings

Research paper thumbnail of Formation of hemoglobin assisted graphene oxide biocomposite film

Indian Journal of Physics

Graphene oxide is a one-atom thick layer of graphite and has evolved as a promising precursor for... more Graphene oxide is a one-atom thick layer of graphite and has evolved as a promising precursor for preparing graphene-based composite materials with wide range of applications. Nowadays studies on the binding of biomolecules and nanoparticles with graphene oxide have become an interesting subject to researchers. In this paper, we report the interaction of graphene oxide with hemoglobin at the air-water interface by Langmuir-Blodgett and spectroscopic techniques. Study of the surface pressure growth kinetics reveals that an increased amount of graphene oxide in water subphase enhances surface activity of hemoglobin. The composite monolayers have been transferred onto glass substrate for characterizations by scanning electron microscopy, atomic force microscopy, Raman spectroscopy, UV-vis absorption and emission spectroscopy and circular dichroism. Studies show the decrement of a-helix with increasing concentration of graphene oxide with no major change in b-sheet, which suggests a modification of secondary structure of hemoglobin due to formation of hemoglobin graphene oxide complex. Hemoglobin changes its conformation in presence of the micro hydrophobic environment of graphene oxide sheet, thus forming the said complex at air-water interface.

Research paper thumbnail of A Review on Protein Based Nanobiocomposite for Biosensor Application

Reviews in Advanced Sciences and Engineering

Research paper thumbnail of Incorporation of pepsin within zwitterionic, anionic, and cationic lipid monolayers: A comparative study

RSC Advances, 2011

The incorporation of water-soluble surface-active enzyme pepsin (PEP) within insoluble zwitterion... more The incorporation of water-soluble surface-active enzyme pepsin (PEP) within insoluble zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and anionic stearic acid (SA) monolayer is studied. Furthermore, the results are compared with cationic octadecylamine (ODA). Adsorption of PEP is found to be higher in ODA as compared to that of DPPC and SA. PEP adsorption kinetics in lipids monolayer follows two-step process: diffusion and unfolding. The unfolding of PEP is lower in the case of DPPC than in SA and ODA. p-A isotherm together with high-resolution field emission scanning electron microscope (FE-SEM) images indicate that at higher pressure, PEP molecules tend to squeeze out from the lipids monolayer. PEP forms larger irregular intermolecular aggregates by increment of b-component on SA and ODA monolayer. However, in zwitterionic (DPPC) matrix the a-helix increases with smaller intermolecular aggregates. The overall results specify that zwitterionic (DPPC) monolayer is better choice to obtain protein lipid mixed film than anionic (SA) and cationic (ODA) monolayer.

Research paper thumbnail of Studies on Protein Conformation in Water and Air/Water Interface by Langmuir Blodgett Technique

This thesis deals with some multi disciplinary work involving the combination of different techni... more This thesis deals with some multi disciplinary work involving the combination of different techniques on the basic study of development and characterization of protein and protein based bioactive thin film at air/water interface and subsequent transfer onto solid substrate.

Research paper thumbnail of Hemoglobin−Silver Interaction and Bioconjugate Formation: A Spectroscopic Study

The Journal of Physical Chemistry B, 2010

In this article, we report the results of the extent of interaction as well as the formation of a... more In this article, we report the results of the extent of interaction as well as the formation of a bioconjugate of human hemoglobin (Hb) with silver (Ag). The complexation process and conformational changes are characterized using different spectroscopic and microscopic techniques. The UV-vis study demonstrates the perturbation of the soret/heme band and generates conformational heterogeneity within the heme group in the presence of silver. A fluorescence study suggests that the Tryptophan (Trp) residues of Hb are in a more polar environment after conjugation. Initial fluorescence enhancement with addition of silver is due to metal-enhanced fluorescence. Moreover, the fluorescence quenching after the formation of the Hb-Ag bioconjugate follows the modified Stern-Volmer (S-V) plot. The S-V plot along with the time-resolved fluorescence study indicates the presence of both static and dynamic types of quenching. In addition, the reduction potential values of the entities (Hb-heme, Ag(+), and Trp) indicate the possible electron transfer. The secondary structure calculation from CD and FTIR spectra indicate alpha-helix to beta-sheet conversion, and unfolding of Hb is also responsible for the bioconjugate formation. In addition, FE-SEM, phase contrast inverted microscopy (PCIM) images demonstrate the formation of the silver-protein bioconjugate. The overall data show that there is a change in the secondary as well as the tertiary structure of Hb after conjugation with silver.

Research paper thumbnail of Effect of Salt on the Formation of Alcohol-Dehydrogenease Monolayer: A Study by the Langmuir−Blodgett Technique

The Journal of Physical Chemistry B, 2009

We report here the effect of salt (KCl) on the interfacial surface activity of yeast alcohol dehy... more We report here the effect of salt (KCl) on the interfacial surface activity of yeast alcohol dehydogenease (ADH) at air/water interface using the Langmuir-Blodgett technique. Effect of salt content in the water subphase on ADH structure has been studied. The change of area/molecule, compressibility, rigidity, and unfolding of ADH are insignificant up to 10 mM KCl concentration. The significant changes are observed above 0.1 M KCl concentrations. Observations are explained in the context of DLVO theory. FTIR study of amide band together with AFM imaging of ADH monolayer indicate that KCl perturbs the ADH monolayer by the increment of-structure resulting into larger unfolding and intermolecular aggregates at high salt concentration.

Research paper thumbnail of Mn-doped SrTiO3 and SrTiO3–Fe2O3 composite perovskites: photocatalytic dye degradation and energy storage application

Journal of Materials Science: Materials in Electronics

Research paper thumbnail of Studies on the interaction and complexation of pyrrole compound with hemoglobin

Department of Chemistry, School of Basic and Applied Sciences, Career Point University, Hamirpur-... more Department of Chemistry, School of Basic and Applied Sciences, Career Point University, Hamirpur-176 041, Himachal Pradesh, India E-mail : sanjitmahato@gmail.com Department of Chemical Engineering, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA), South Africa Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi-835 215, Jharkhand, India Physics Division, Department of Basic Sciences and Social Sciences, School of Technology, North Eastern Hill University, Shillong-793 022, Meghalaya, India <em>E-mail</em> : mrityunjoyphy@gmail.com DST/CSIR Innovation Centre, National Centre for Nanostructured Materials, CSIR Material Science and Manufacturing, Building-19B, PO Box 395, Pretoria-0001, South Africa Currently Research Scientist at TCG Lifesciences Pvt. Ltd., Kolkata-700 091, India <em>Manuscript received 23 June 2017, accepted 15 July 2017</em> Alkaloids such as pyrrole, tetrap...

Research paper thumbnail of Biosensors for detection of prostate cancer: a review

Research paper thumbnail of Studies on protein conformation in water and air water interface by Langmuir Blodgett technique

This thesis deals with some multi disciplinary work involving the combination of different techni... more This thesis deals with some multi disciplinary work involving the combination of different techniques on the basic study of development and characterization of protein and protein based bioactive thin film at air/water interface and subsequent transfer onto solid substrate.

Research paper thumbnail of Waste plastic-derived NiO-MWCNT composite as visible light photocatalyst for degradation of methylene blue dye

Nanotechnology for Environmental Engineering

Research paper thumbnail of Recent Advances on Polymer Nanocomposite-Based Radiation Shielding Materials for Medical Science

Nowadays, radiation is an integral part of our lives and economy by means of natural and human ac... more Nowadays, radiation is an integral part of our lives and economy by means of natural and human activity such as UV radiation (forensic analysis, medical imaging) (www.sciencing.com), microwave radiation (mobile phone, radar) (www.ffden-2.phys.uaf.edu), X-ray (radiology, security scan, material analysis) (www.labmate-online.com), and alpha, beta, gamma radiations (nuclear medicine, radiotherapy) (www.bbc.co.uk). The unwanted exposure to these radiations can lead to health disorder (cancer, birth defect) and other kinds of accidents. Therefore, protection of our health and environment from the radiation is necessary, and development of radiation protection nanocomposite is now an emergent field of research. Usually, a high-density material lead is used to protect in general from any kind of radiation and in particular from nuclear radiation. However, due to high atomic weight and high toxicity of lead, it has limited applications, and researchers are encouraged to look for a lightweig...

Research paper thumbnail of Herbal Nanocarriers for Cancer Therapy

Cancer is a group of around 100 diseases that has been tormenting mankind since ancient time. Due... more Cancer is a group of around 100 diseases that has been tormenting mankind since ancient time. Due to cancer, estimated 8.2 million people died globally in 2012, and the toll is expected to reach 13 million in 2030. Despite the improvement of conventional therapeutic modalities, the outcome of cancer patients has not improved significantly. So, alternative therapeutic modalities and new effective anticancer drugs are highly sought for. Different parts of plants and their extracts have been used to cure many diseases and relive from physical agony since ancient times. In the traditional system of medicine, herbal products have been used for treating different types of diseases and alignments globally. Active compounds from herbal medicine, such as curcumin, are found to be effective against cancer. Despite their excellent therapeutic ability, the potential of these herbal compounds or phytochemicals is limited due to their low water solubility and poor bioavailability.

Research paper thumbnail of Measurement of elastic modulus of CNT composites: a nondestructive study

Fullerenes, Nanotubes and Carbon Nanostructures

CNT based nanocomposites are known to have superior mechanical properties, such as elastic modulu... more CNT based nanocomposites are known to have superior mechanical properties, such as elastic modulus (E), tensile strength (σ), etc., compared to other nanocomposite materials. Traditionally E of the...

Research paper thumbnail of Paper-Based Sensors for Biomedical Applications

Biomedical Engineering and its Applications in Healthcare

Paper-based sensor is a new analytical technique applicable in healthcare monitoring, environment... more Paper-based sensor is a new analytical technique applicable in healthcare monitoring, environmental monitoring, food quality control, etc. Currently, these technologies are drawing great attention due to ease of fabrication, cost-effectiveness, and their simple, portable, and disposable nature. The inherent qualities of paper, such as availability in large quantities, cheap, lightweight, and biodegradability, are being exploited to develop sustainable devices. The application of paper as a substrate material in litmus paper was dated back to seventeenth century. The semiquantitative detection of glucose in urine and immune chromatographic paper test strips for pregnancy test kits are examples of paper-based sensing devices. Today, microfluidic paper-based sensors with advanced designs involving complex 3D geometrics for handling multiples analytes are under investigation. It is foreseen that advancements in the fabrication and analytical techniques will help in improving the accuracy and sensitivity of paper-based sensors. Different types of detection approaches used in microfluidic paper-based devices are colorimetry, luminescence, electrochemical detection, fluorescence, and surface-enhanced Raman scattering.

Research paper thumbnail of Metalloprotein-Inspired Ruthenium Polymeric Complex: A Highly Efficient Catalyst in Parts per Million Level for 1,3-Dipolar Huisgen’s Reaction in Aqueous Medium at Room Temperature

Industrial & Engineering Chemistry Research, 2017

Research paper thumbnail of Waste derived low cost PbO-Carbon nanocomposite and its energy storage application

Materials Today: Proceedings

Research paper thumbnail of Preparation, characterization and some electrochemical study of waste derived iron Oxide-Carbon nanocomposite

Materials Today: Proceedings

Research paper thumbnail of Conversion of Solid Waste into Functional Carbon Materials: A Review

Energy and Environment Focus

Research paper thumbnail of Biomineralized Nanocrystal of Calcium Carbonate in Protein Langmuir Blodgett Monolayer

Materials Today: Proceedings

Research paper thumbnail of Formation of hemoglobin assisted graphene oxide biocomposite film

Indian Journal of Physics

Graphene oxide is a one-atom thick layer of graphite and has evolved as a promising precursor for... more Graphene oxide is a one-atom thick layer of graphite and has evolved as a promising precursor for preparing graphene-based composite materials with wide range of applications. Nowadays studies on the binding of biomolecules and nanoparticles with graphene oxide have become an interesting subject to researchers. In this paper, we report the interaction of graphene oxide with hemoglobin at the air-water interface by Langmuir-Blodgett and spectroscopic techniques. Study of the surface pressure growth kinetics reveals that an increased amount of graphene oxide in water subphase enhances surface activity of hemoglobin. The composite monolayers have been transferred onto glass substrate for characterizations by scanning electron microscopy, atomic force microscopy, Raman spectroscopy, UV-vis absorption and emission spectroscopy and circular dichroism. Studies show the decrement of a-helix with increasing concentration of graphene oxide with no major change in b-sheet, which suggests a modification of secondary structure of hemoglobin due to formation of hemoglobin graphene oxide complex. Hemoglobin changes its conformation in presence of the micro hydrophobic environment of graphene oxide sheet, thus forming the said complex at air-water interface.

Research paper thumbnail of A Review on Protein Based Nanobiocomposite for Biosensor Application

Reviews in Advanced Sciences and Engineering

Research paper thumbnail of Incorporation of pepsin within zwitterionic, anionic, and cationic lipid monolayers: A comparative study

RSC Advances, 2011

The incorporation of water-soluble surface-active enzyme pepsin (PEP) within insoluble zwitterion... more The incorporation of water-soluble surface-active enzyme pepsin (PEP) within insoluble zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and anionic stearic acid (SA) monolayer is studied. Furthermore, the results are compared with cationic octadecylamine (ODA). Adsorption of PEP is found to be higher in ODA as compared to that of DPPC and SA. PEP adsorption kinetics in lipids monolayer follows two-step process: diffusion and unfolding. The unfolding of PEP is lower in the case of DPPC than in SA and ODA. p-A isotherm together with high-resolution field emission scanning electron microscope (FE-SEM) images indicate that at higher pressure, PEP molecules tend to squeeze out from the lipids monolayer. PEP forms larger irregular intermolecular aggregates by increment of b-component on SA and ODA monolayer. However, in zwitterionic (DPPC) matrix the a-helix increases with smaller intermolecular aggregates. The overall results specify that zwitterionic (DPPC) monolayer is better choice to obtain protein lipid mixed film than anionic (SA) and cationic (ODA) monolayer.

Research paper thumbnail of Studies on Protein Conformation in Water and Air/Water Interface by Langmuir Blodgett Technique

This thesis deals with some multi disciplinary work involving the combination of different techni... more This thesis deals with some multi disciplinary work involving the combination of different techniques on the basic study of development and characterization of protein and protein based bioactive thin film at air/water interface and subsequent transfer onto solid substrate.

Research paper thumbnail of Hemoglobin−Silver Interaction and Bioconjugate Formation: A Spectroscopic Study

The Journal of Physical Chemistry B, 2010

In this article, we report the results of the extent of interaction as well as the formation of a... more In this article, we report the results of the extent of interaction as well as the formation of a bioconjugate of human hemoglobin (Hb) with silver (Ag). The complexation process and conformational changes are characterized using different spectroscopic and microscopic techniques. The UV-vis study demonstrates the perturbation of the soret/heme band and generates conformational heterogeneity within the heme group in the presence of silver. A fluorescence study suggests that the Tryptophan (Trp) residues of Hb are in a more polar environment after conjugation. Initial fluorescence enhancement with addition of silver is due to metal-enhanced fluorescence. Moreover, the fluorescence quenching after the formation of the Hb-Ag bioconjugate follows the modified Stern-Volmer (S-V) plot. The S-V plot along with the time-resolved fluorescence study indicates the presence of both static and dynamic types of quenching. In addition, the reduction potential values of the entities (Hb-heme, Ag(+), and Trp) indicate the possible electron transfer. The secondary structure calculation from CD and FTIR spectra indicate alpha-helix to beta-sheet conversion, and unfolding of Hb is also responsible for the bioconjugate formation. In addition, FE-SEM, phase contrast inverted microscopy (PCIM) images demonstrate the formation of the silver-protein bioconjugate. The overall data show that there is a change in the secondary as well as the tertiary structure of Hb after conjugation with silver.