Ms. Sneha Nayak NMAMIT BT (original) (raw)
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Papers by Ms. Sneha Nayak NMAMIT BT
The manual selection of transformed bacterial colonies from non-transformed ones grown on agar pl... more The manual selection of transformed bacterial colonies from non-transformed ones grown on agar plate post-blue-white screening – despite chromogenic difference – is cumbersome, owing to their small size and large cell number. The present study offers a lucrative solution to this problem by the design of an automated system that is not only fast and less laborious but also low priced and user-friendly. The image masking technique was used to distinguish plated transformed colonies. This method uses computer vision techniques to detect the number of blue and white colonies post bacterial transformation on agar plates and calculate the transformation efficiency. To assess the proposed model with the manual counting method, we have validated the model by comparing the manual counting of colonies with the automated system count. Hence, a model was developed that would be an added advantage to biotechnologists as it would minimize the time required for counting and help in productive rese...
BioNanoScience
Nowadays, biological materials are explored widely for green synthesis of nanoparticles because o... more Nowadays, biological materials are explored widely for green synthesis of nanoparticles because of its ease in scaling up when compared with conventional approaches. In this paper, we report the biosynthesis of bimetallic nanoparticles (FeCuNPs) from their precursors FeSO4 and CuSO4, using Cyclea peltata leaf extract. Biosynthesized nanoparticles were characterized by UV-visible spectroscopy, particle size analyser, FESEM, EDAX, XRD and FTIR. UV-visible spectroscopic investigation confirmed the production of bimetallic core shell nanoparticles at 250 nm, where the colour change in the solution indicated the formation of nanoparticles. The synthesized FeCuNPs were tested for their methyl green dye degradation and degradation kinetics. Results indicated that bimetallic nanoparticles could effectively degrade methyl green dye up to 82% within 105 min, which followed pseudo second order kinetics with R2 of 0.9862. Hence time-dependent reduction in methyl green absorption maxima, obtained from UV-Spectrophotometric analysis and LCMS spectra of degraded dye, confirmed that the green synthesized bimetallic nanoparticles from the leaf extract of Cyclea peltata has the potential of dye degradation.
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects
The manual selection of transformed bacterial colonies from non-transformed ones grown on agar pl... more The manual selection of transformed bacterial colonies from non-transformed ones grown on agar plate post-blue-white screening – despite chromogenic difference – is cumbersome, owing to their small size and large cell number. The present study offers a lucrative solution to this problem by the design of an automated system that is not only fast and less laborious but also low priced and user-friendly. The image masking technique was used to distinguish plated transformed colonies. This method uses computer vision techniques to detect the number of blue and white colonies post bacterial transformation on agar plates and calculate the transformation efficiency. To assess the proposed model with the manual counting method, we have validated the model by comparing the manual counting of colonies with the automated system count. Hence, a model was developed that would be an added advantage to biotechnologists as it would minimize the time required for counting and help in productive rese...
BioNanoScience
Nowadays, biological materials are explored widely for green synthesis of nanoparticles because o... more Nowadays, biological materials are explored widely for green synthesis of nanoparticles because of its ease in scaling up when compared with conventional approaches. In this paper, we report the biosynthesis of bimetallic nanoparticles (FeCuNPs) from their precursors FeSO4 and CuSO4, using Cyclea peltata leaf extract. Biosynthesized nanoparticles were characterized by UV-visible spectroscopy, particle size analyser, FESEM, EDAX, XRD and FTIR. UV-visible spectroscopic investigation confirmed the production of bimetallic core shell nanoparticles at 250 nm, where the colour change in the solution indicated the formation of nanoparticles. The synthesized FeCuNPs were tested for their methyl green dye degradation and degradation kinetics. Results indicated that bimetallic nanoparticles could effectively degrade methyl green dye up to 82% within 105 min, which followed pseudo second order kinetics with R2 of 0.9862. Hence time-dependent reduction in methyl green absorption maxima, obtained from UV-Spectrophotometric analysis and LCMS spectra of degraded dye, confirmed that the green synthesized bimetallic nanoparticles from the leaf extract of Cyclea peltata has the potential of dye degradation.
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects