Muhammad Anees Aslam - Academia.edu (original) (raw)
Papers by Muhammad Anees Aslam
Land
Climate change is likely to have serious social, economic, and environmental impacts on farmers w... more Climate change is likely to have serious social, economic, and environmental impacts on farmers whose subsistence depends on nature. Land Use Land Cover (LULC) changes were examined as a significant tool for assessing changes at diverse temporal and spatial scales. Normalized Difference Vegetation Index (NDVI) has the potential ability to signify the vegetation structures of various eco-regions and provide valuable information as a remote sensing tool in studying vegetation phenology cycles. In this study, we used remote sensing and Geographical Information System (GIS) techniques with Maximum Likelihood Classification (MLC) to identify the LULC changes for 40 years in the Sahiwal District. Later, we conducted 120 questionnaires administered to local farmers which were used to correlate climate changes with NDVI. The LULC maps were prepared using MLC and training sites for the years 1981, 2001, and 2021. Regression analysis (R2) was performed to identify the relationship between tem...
Complexity, 2021
The need for smart e-learning environments is resulting in new challenges for researchers and pra... more The need for smart e-learning environments is resulting in new challenges for researchers and practitioners to develop intelligent systems that can be used to automate the Higher Education (HE) activities in an intelligent way. Some common examples of such activities are “analyzing, finding, and ranking the right resource to teach a course,” “analyzing and finding the people with common research interests to start joint research projects,” and “using data analytics and machine reasoning techniques for conducting the exams with different levels of complexities.” Ontological reasoning and smart data analytics can play an important role in analyzing and automating these HE activities and processes. In this paper, we present a framework named as Higher Education Activities and Processes Automation Framework (HEAPAF). The HEAPAF framework can be used to identify, extract, process, and produce the semantically enriched data in machine understandable format from different educational resou...
International Journal of Peptide Research and Therapeutics, 2022
Antimicrobial peptides (AMPs) are endogenous polypeptides produced by multicellular organisms in ... more Antimicrobial peptides (AMPs) are endogenous polypeptides produced by multicellular organisms in order to protect a host from pathogenic microbes. AMPs are also defined as host defense peptides because of their essential role in constituting the innate immunity system. The major mechanism of actions against microbial pathogens is attributed to perturbation of cell membrane of pathogens. In addition to the antimicrobial function, AMPs also act as effector molecules in wound healing, inflammation, and immune activation. AMPs have been considered as a potential therapeutic source due to its different mechanism of actions and broad-spectrum properties in comparison with the conventionally used antibiotics. As a new generation antibiotic, the AMPs have considerable benefits. AMPs are receiving great attention as promising alternatives to conventional antibiotics to overcome the current drug resistance crisis. In this review, we describe the classes, structural information about AMPs and their therapeutic role as novel antibiotic agents.
Information is one of the most important resources for organizations as well as for individuals. ... more Information is one of the most important resources for organizations as well as for individuals. Keeping the information (e.g. credit card information, images that represent organizational internal processes, organizational strategic documents etc.) secured from unauthorized resources is becoming one of the key challenges of the current age, especially when information is being exchanged very frequently over the Internet. For many years people are concern with privacy and security but with this revelation of information and the social networks privacy became real concern. One of the main reasons is that when we share the information (in the form of images or documents) with our known contacts then actually we are also sharing the information with the telecommunication companies and the company that provide the software. In this paper we present a symmetric key encryption based solution for information privacy and security. Our approach is based on Advanced Encryption Standard (AES) ...
El presente ensayo expone los complejos procesos de reinvencion que llevan a cabo los grupos indi... more El presente ensayo expone los complejos procesos de reinvencion que llevan a cabo los grupos indigenas estadounidenses desplazados a las ciudades. Nos interesa particularmente la negociacion de fronteras etnicas que se efectua en el contexto de los powwows, celebraciones de ritualidad secular que originalmente se circunscribian a las reservaciones de las planicies, pero que hoy se realizan tambien en muchas ciudades. Se toma como ejemplo de caso el powwow celebrado anualmente en las instalaciones de la YMCA de la ciudad de Nueva York, en el que participan miembros de diferentes tribus, pero mayoritariamente gente no indigena que tiene diferentes niveles de identificacion con esos grupos. Dos preguntas fundamentales dirigen estas reflexiones; ?que papel juegan los powwows en el proceso de mantener, imaginar y reinventar a la comunidad indigena? ?Como se negocian las fronteras etnicas durante el performance o ejecucion de un powwow, y que conflictos internos puede generar se...
PeerJ Computer Science, 2021
Background Results of scientific experiments and research work, either conducted by individuals o... more Background Results of scientific experiments and research work, either conducted by individuals or organizations, are published and shared with scientific community in different types of scientific publications such as books, chapters, journals, articles, reference works and reference works entries. One aspect of these documents is their contents and the other is metadata. Metadata of scientific documents could be used to increase mutual cooperation, find people with common interest and research work, and to find scientific documents in the matching domains. The major issue in getting these benefits from metadata of scientific publications is availability of these data in unstructured (or semi-structured) format so that it can not be used to ask smart queries that can help in computing and performing different types of analysis on scientific publications data. Also, acquisition and smart processing of publications data is a complicated as well as time and resource consuming task. Me...
Sustainability, 2020
Due to the wide-ranging development of data-oriented sustainable systems in the government and th... more Due to the wide-ranging development of data-oriented sustainable systems in the government and the public sectors, the development of such sustainable systems is replete with potential. The ultimate focus of developing these sustainable systems is to provide citizens with transparency, accountability, awareness as well as a single point of query for asking integrated and smart queries. In view of these benefits, the Saudi government has taken the initiative to publish and develop sustainable open data-oriented information systems. However some major challenges in the Saudi Government Open Data are that the (1) data are published and available in different formats such as Excel sheets, CSV files (Comma Separated Values), images, scanned documents and social media sources such as Twitter, (2) datasets from different government departments are not linked with each other or to existing datasets in Linked Open Data Cloud (even though they have strong links with each other), and (3) there...
Sustainability, 2020
An emerging technology with a secure and a decentralized nature, blockchain has the potential to ... more An emerging technology with a secure and a decentralized nature, blockchain has the potential to transform conventional practices in an efficient and dynamic manner. However, migrating to blockchain can be challenging due to the complexity of its infrastructure and processes. The complexity of building applications on blockchain has been highlighted by many studies, thus stressing the need to investigate practical solutions further. A commonly known software engineering concept, software design pattern contributes to the acceleration of software development. It offers a holistic reusable solution for commonly occurring problems in a given context. It helps to identify problems that occur repetitively and describes best practices to address them. The present study is one of the first investigations to inquire into design patterns for blockchain application. Seeking to reduce the complexity in understanding and building applications on blockchain, this paper identifies a design patter...
International Journal of Information Engineering and Electronic Business, 2018
Scientific Reports, 2019
Atypical parkinsonian disorders (APDs) comprise a group of neurodegenerative diseases with hetero... more Atypical parkinsonian disorders (APDs) comprise a group of neurodegenerative diseases with heterogeneous clinical and pathological features. Most APDs are sporadic, but rare familial forms have also been reported. Epidemiological and post-mortem studies associated APDs with oxidative stress and cellular protein aggregates. Identifying molecular mechanisms that translate stress into toxic protein aggregation and neurodegeneration in APDs is an active area of research. Recently, ribonucleic acid (RNA) stress granule (SG) pathways were discussed to be pathogenically relevant in several neurodegenerative disorders including APDs. Using whole genome sequencing, mRNA expression analysis, transfection assays and cell imaging, we investigated the genetic and molecular basis of a familial neurodegenerative atypical parkinsonian disorder. We investigated a family with six living members in two generations exhibiting clinical symptoms consistent with atypical parkinsonism. Two affected family ...
Sustainability, 2019
With the advancement of technology, academics and curriculum developers are always under pressure... more With the advancement of technology, academics and curriculum developers are always under pressure to provide students with skills that match the market’s requirements. A systematic and continuous examination of the market is needed, to stay up to date with the required skills, and then to update the curriculum to train the students with required market skills. In this article, we present a framework referred to as Align My Curriculum (AMC). The AMC framework aims to facilitate alignment between acquired university curriculum outcomes and required market skills. It can be used to classify, compare and visualize the data of a university curriculum and job vacancies in the market. The presented framework benefits academics and curriculum developers by improving the courses and therefore bridging the skills gap. Stakeholders from both academia and industry can gain insights into the predominant required and acquired skills. In addition, it may be useful for analysts, students, and job a...
Program, 2017
Purpose Tags are used to annotate resources on social media platforms. Most tag recommendation me... more Purpose Tags are used to annotate resources on social media platforms. Most tag recommendation methods use popular tags, but in the case of new resources that are as yet untagged (the cold start problem), popularity-based tag recommendation methods fail to work. The purpose of this paper is to propose a novel model for tag recommendation called multi-feature space latent Dirichlet allocation (MFS-LDA) for cold start problem. Design/methodology/approach MFS-LDA is a novel latent Dirichlet allocation (LDA)-based model which exploits multiple feature spaces (title, contents, and tags) for recommending tags. Exploiting multiple feature spaces allows MFS-LDA to recommend tags even if data from a feature space is missing (the cold start problem). Findings Evaluation of a publicly available data set consisting of around 20,000 Wikipedia articles that are tagged on a social bookmarking website shows a significant improvement over existing LDA-based tag recommendation methods. Originality/va...
Sarhad Journal of Agriculture, 2016
Web-Age Information Management, 2016
There is a noticeable increase in the number of scientific publications. These publications are b... more There is a noticeable increase in the number of scientific publications. These publications are being published by different publishers. Springer is one of those publishers which has published more than nine million scientific documents. SpringerLink is the portal providing the gateway to searching and accessing these published scientific documents. The structure, as well as the way, the contents are presented on the portal, provides valuable information about documents metadata such as author, ISBN, references, articles, chapters. However, this metadata is understandable by human in such a way that it facilitates the keyword-based searches through SpringerLink portal. At the same time this huge data about scientific documents is in silence as it is neither open nor linked to other datasets. To address these issues, we have created a semantics based repository called SPedia which consists of semantically enriched data about documents published by Springer. Currently, SPedia datasets consist of more than 300 million RDF triples. In this paper we describe SPedia and examine the quality of its extracted data by performing semantically enriched queries. The results show that SPedia facilitates the users to put sophisticated queries by employing semantic Web techniques instead of keyword-based searches. In addition, SPedia datasets can be utilized to link to other datasets available in the Linked Open Data (LOD) cloud.
Nóesis. Revista de Ciencias Sociales y Humanidades, 2015
British journal of cancer, Jan 13, 2010
Primary radiotherapy (RT) is a mainstay of treatment for laryngeal squamous cell carcinoma (LSCC)... more Primary radiotherapy (RT) is a mainstay of treatment for laryngeal squamous cell carcinoma (LSCC). Although the cure rates for early (T1) vocal cord tumours are high, RT proves ineffective in up to a third of T3 carcinomas. Moreover, RT is associated with debilitating early- and late-treatment-related toxicity, thus finding means to de-escalate therapy, while retaining/augmenting therapeutic effectiveness, is highly desirable. p53 is a key mediator of radiation responses; we therefore investigated whether Nutlin-3, a small-molecule inhibitor of MDM2 (mouse double minute 2; an essential negative regulator of p53), might radiosensitise LSCC cells. We performed clonogenic assays to measure radiosensitivity in a panel of LSCC cell lines (for which we determined p53 mutational status) in the presence and absence of Nutlin-3. LSCC cells harbouring wild-type p53 were significantly radiosensitised by Nutlin-3 (P<0.0001; log-rank scale), and displayed increased cell cycle arrest and signi...
Business Process Management Workshops, 2006
Global Journal of Health Science, 2011
Public Health Genomics, 2008
We report cascade testing of a large Pakistani family for beta-thalassemia alleles. The family wa... more We report cascade testing of a large Pakistani family for beta-thalassemia alleles. The family was still practicing consanguineous marriages and was at risk of having more affected births. The objective of this study was to show that identification of disease carriers in families with index cases in order to create awareness about disease and provide genetic counseling would result in reduction of the frequency of beta-thalassemia in Pakistan. In this large family with an index case, 27 available living members were tested for beta-thalassemia. Carriers of the disease were detected by measuring hemoglobin indices, and amplification refractory mutation system polymerase chain reaction was used for mutation analysis. Genetic counseling was provided to members of this family. There were already 3 marriages between the carrier members and 1 between a carrier and noncarrier in this large family; 12 (44.4%) members were found to carry the mutant gene, representing a very high carrier rate compared to the 5.4% carrier frequency of beta-thalassemia in the general population of Pakistan. The family was counseled for prevention of affected births. The initially reluctant family gradually became cooperative and seriously attended the genetic counseling sessions. Cascade testing is more practical than general population screening in a country with limited health facilities where consanguineous marriages are practiced. This report emphasizes the need of extensive testing within families with index cases to identify the carriers of beta-thalassemia in order to reduce disease occurrence through awareness and genetic counseling.
Land
Climate change is likely to have serious social, economic, and environmental impacts on farmers w... more Climate change is likely to have serious social, economic, and environmental impacts on farmers whose subsistence depends on nature. Land Use Land Cover (LULC) changes were examined as a significant tool for assessing changes at diverse temporal and spatial scales. Normalized Difference Vegetation Index (NDVI) has the potential ability to signify the vegetation structures of various eco-regions and provide valuable information as a remote sensing tool in studying vegetation phenology cycles. In this study, we used remote sensing and Geographical Information System (GIS) techniques with Maximum Likelihood Classification (MLC) to identify the LULC changes for 40 years in the Sahiwal District. Later, we conducted 120 questionnaires administered to local farmers which were used to correlate climate changes with NDVI. The LULC maps were prepared using MLC and training sites for the years 1981, 2001, and 2021. Regression analysis (R2) was performed to identify the relationship between tem...
Complexity, 2021
The need for smart e-learning environments is resulting in new challenges for researchers and pra... more The need for smart e-learning environments is resulting in new challenges for researchers and practitioners to develop intelligent systems that can be used to automate the Higher Education (HE) activities in an intelligent way. Some common examples of such activities are “analyzing, finding, and ranking the right resource to teach a course,” “analyzing and finding the people with common research interests to start joint research projects,” and “using data analytics and machine reasoning techniques for conducting the exams with different levels of complexities.” Ontological reasoning and smart data analytics can play an important role in analyzing and automating these HE activities and processes. In this paper, we present a framework named as Higher Education Activities and Processes Automation Framework (HEAPAF). The HEAPAF framework can be used to identify, extract, process, and produce the semantically enriched data in machine understandable format from different educational resou...
International Journal of Peptide Research and Therapeutics, 2022
Antimicrobial peptides (AMPs) are endogenous polypeptides produced by multicellular organisms in ... more Antimicrobial peptides (AMPs) are endogenous polypeptides produced by multicellular organisms in order to protect a host from pathogenic microbes. AMPs are also defined as host defense peptides because of their essential role in constituting the innate immunity system. The major mechanism of actions against microbial pathogens is attributed to perturbation of cell membrane of pathogens. In addition to the antimicrobial function, AMPs also act as effector molecules in wound healing, inflammation, and immune activation. AMPs have been considered as a potential therapeutic source due to its different mechanism of actions and broad-spectrum properties in comparison with the conventionally used antibiotics. As a new generation antibiotic, the AMPs have considerable benefits. AMPs are receiving great attention as promising alternatives to conventional antibiotics to overcome the current drug resistance crisis. In this review, we describe the classes, structural information about AMPs and their therapeutic role as novel antibiotic agents.
Information is one of the most important resources for organizations as well as for individuals. ... more Information is one of the most important resources for organizations as well as for individuals. Keeping the information (e.g. credit card information, images that represent organizational internal processes, organizational strategic documents etc.) secured from unauthorized resources is becoming one of the key challenges of the current age, especially when information is being exchanged very frequently over the Internet. For many years people are concern with privacy and security but with this revelation of information and the social networks privacy became real concern. One of the main reasons is that when we share the information (in the form of images or documents) with our known contacts then actually we are also sharing the information with the telecommunication companies and the company that provide the software. In this paper we present a symmetric key encryption based solution for information privacy and security. Our approach is based on Advanced Encryption Standard (AES) ...
El presente ensayo expone los complejos procesos de reinvencion que llevan a cabo los grupos indi... more El presente ensayo expone los complejos procesos de reinvencion que llevan a cabo los grupos indigenas estadounidenses desplazados a las ciudades. Nos interesa particularmente la negociacion de fronteras etnicas que se efectua en el contexto de los powwows, celebraciones de ritualidad secular que originalmente se circunscribian a las reservaciones de las planicies, pero que hoy se realizan tambien en muchas ciudades. Se toma como ejemplo de caso el powwow celebrado anualmente en las instalaciones de la YMCA de la ciudad de Nueva York, en el que participan miembros de diferentes tribus, pero mayoritariamente gente no indigena que tiene diferentes niveles de identificacion con esos grupos. Dos preguntas fundamentales dirigen estas reflexiones; ?que papel juegan los powwows en el proceso de mantener, imaginar y reinventar a la comunidad indigena? ?Como se negocian las fronteras etnicas durante el performance o ejecucion de un powwow, y que conflictos internos puede generar se...
PeerJ Computer Science, 2021
Background Results of scientific experiments and research work, either conducted by individuals o... more Background Results of scientific experiments and research work, either conducted by individuals or organizations, are published and shared with scientific community in different types of scientific publications such as books, chapters, journals, articles, reference works and reference works entries. One aspect of these documents is their contents and the other is metadata. Metadata of scientific documents could be used to increase mutual cooperation, find people with common interest and research work, and to find scientific documents in the matching domains. The major issue in getting these benefits from metadata of scientific publications is availability of these data in unstructured (or semi-structured) format so that it can not be used to ask smart queries that can help in computing and performing different types of analysis on scientific publications data. Also, acquisition and smart processing of publications data is a complicated as well as time and resource consuming task. Me...
Sustainability, 2020
Due to the wide-ranging development of data-oriented sustainable systems in the government and th... more Due to the wide-ranging development of data-oriented sustainable systems in the government and the public sectors, the development of such sustainable systems is replete with potential. The ultimate focus of developing these sustainable systems is to provide citizens with transparency, accountability, awareness as well as a single point of query for asking integrated and smart queries. In view of these benefits, the Saudi government has taken the initiative to publish and develop sustainable open data-oriented information systems. However some major challenges in the Saudi Government Open Data are that the (1) data are published and available in different formats such as Excel sheets, CSV files (Comma Separated Values), images, scanned documents and social media sources such as Twitter, (2) datasets from different government departments are not linked with each other or to existing datasets in Linked Open Data Cloud (even though they have strong links with each other), and (3) there...
Sustainability, 2020
An emerging technology with a secure and a decentralized nature, blockchain has the potential to ... more An emerging technology with a secure and a decentralized nature, blockchain has the potential to transform conventional practices in an efficient and dynamic manner. However, migrating to blockchain can be challenging due to the complexity of its infrastructure and processes. The complexity of building applications on blockchain has been highlighted by many studies, thus stressing the need to investigate practical solutions further. A commonly known software engineering concept, software design pattern contributes to the acceleration of software development. It offers a holistic reusable solution for commonly occurring problems in a given context. It helps to identify problems that occur repetitively and describes best practices to address them. The present study is one of the first investigations to inquire into design patterns for blockchain application. Seeking to reduce the complexity in understanding and building applications on blockchain, this paper identifies a design patter...
International Journal of Information Engineering and Electronic Business, 2018
Scientific Reports, 2019
Atypical parkinsonian disorders (APDs) comprise a group of neurodegenerative diseases with hetero... more Atypical parkinsonian disorders (APDs) comprise a group of neurodegenerative diseases with heterogeneous clinical and pathological features. Most APDs are sporadic, but rare familial forms have also been reported. Epidemiological and post-mortem studies associated APDs with oxidative stress and cellular protein aggregates. Identifying molecular mechanisms that translate stress into toxic protein aggregation and neurodegeneration in APDs is an active area of research. Recently, ribonucleic acid (RNA) stress granule (SG) pathways were discussed to be pathogenically relevant in several neurodegenerative disorders including APDs. Using whole genome sequencing, mRNA expression analysis, transfection assays and cell imaging, we investigated the genetic and molecular basis of a familial neurodegenerative atypical parkinsonian disorder. We investigated a family with six living members in two generations exhibiting clinical symptoms consistent with atypical parkinsonism. Two affected family ...
Sustainability, 2019
With the advancement of technology, academics and curriculum developers are always under pressure... more With the advancement of technology, academics and curriculum developers are always under pressure to provide students with skills that match the market’s requirements. A systematic and continuous examination of the market is needed, to stay up to date with the required skills, and then to update the curriculum to train the students with required market skills. In this article, we present a framework referred to as Align My Curriculum (AMC). The AMC framework aims to facilitate alignment between acquired university curriculum outcomes and required market skills. It can be used to classify, compare and visualize the data of a university curriculum and job vacancies in the market. The presented framework benefits academics and curriculum developers by improving the courses and therefore bridging the skills gap. Stakeholders from both academia and industry can gain insights into the predominant required and acquired skills. In addition, it may be useful for analysts, students, and job a...
Program, 2017
Purpose Tags are used to annotate resources on social media platforms. Most tag recommendation me... more Purpose Tags are used to annotate resources on social media platforms. Most tag recommendation methods use popular tags, but in the case of new resources that are as yet untagged (the cold start problem), popularity-based tag recommendation methods fail to work. The purpose of this paper is to propose a novel model for tag recommendation called multi-feature space latent Dirichlet allocation (MFS-LDA) for cold start problem. Design/methodology/approach MFS-LDA is a novel latent Dirichlet allocation (LDA)-based model which exploits multiple feature spaces (title, contents, and tags) for recommending tags. Exploiting multiple feature spaces allows MFS-LDA to recommend tags even if data from a feature space is missing (the cold start problem). Findings Evaluation of a publicly available data set consisting of around 20,000 Wikipedia articles that are tagged on a social bookmarking website shows a significant improvement over existing LDA-based tag recommendation methods. Originality/va...
Sarhad Journal of Agriculture, 2016
Web-Age Information Management, 2016
There is a noticeable increase in the number of scientific publications. These publications are b... more There is a noticeable increase in the number of scientific publications. These publications are being published by different publishers. Springer is one of those publishers which has published more than nine million scientific documents. SpringerLink is the portal providing the gateway to searching and accessing these published scientific documents. The structure, as well as the way, the contents are presented on the portal, provides valuable information about documents metadata such as author, ISBN, references, articles, chapters. However, this metadata is understandable by human in such a way that it facilitates the keyword-based searches through SpringerLink portal. At the same time this huge data about scientific documents is in silence as it is neither open nor linked to other datasets. To address these issues, we have created a semantics based repository called SPedia which consists of semantically enriched data about documents published by Springer. Currently, SPedia datasets consist of more than 300 million RDF triples. In this paper we describe SPedia and examine the quality of its extracted data by performing semantically enriched queries. The results show that SPedia facilitates the users to put sophisticated queries by employing semantic Web techniques instead of keyword-based searches. In addition, SPedia datasets can be utilized to link to other datasets available in the Linked Open Data (LOD) cloud.
Nóesis. Revista de Ciencias Sociales y Humanidades, 2015
British journal of cancer, Jan 13, 2010
Primary radiotherapy (RT) is a mainstay of treatment for laryngeal squamous cell carcinoma (LSCC)... more Primary radiotherapy (RT) is a mainstay of treatment for laryngeal squamous cell carcinoma (LSCC). Although the cure rates for early (T1) vocal cord tumours are high, RT proves ineffective in up to a third of T3 carcinomas. Moreover, RT is associated with debilitating early- and late-treatment-related toxicity, thus finding means to de-escalate therapy, while retaining/augmenting therapeutic effectiveness, is highly desirable. p53 is a key mediator of radiation responses; we therefore investigated whether Nutlin-3, a small-molecule inhibitor of MDM2 (mouse double minute 2; an essential negative regulator of p53), might radiosensitise LSCC cells. We performed clonogenic assays to measure radiosensitivity in a panel of LSCC cell lines (for which we determined p53 mutational status) in the presence and absence of Nutlin-3. LSCC cells harbouring wild-type p53 were significantly radiosensitised by Nutlin-3 (P<0.0001; log-rank scale), and displayed increased cell cycle arrest and signi...
Business Process Management Workshops, 2006
Global Journal of Health Science, 2011
Public Health Genomics, 2008
We report cascade testing of a large Pakistani family for beta-thalassemia alleles. The family wa... more We report cascade testing of a large Pakistani family for beta-thalassemia alleles. The family was still practicing consanguineous marriages and was at risk of having more affected births. The objective of this study was to show that identification of disease carriers in families with index cases in order to create awareness about disease and provide genetic counseling would result in reduction of the frequency of beta-thalassemia in Pakistan. In this large family with an index case, 27 available living members were tested for beta-thalassemia. Carriers of the disease were detected by measuring hemoglobin indices, and amplification refractory mutation system polymerase chain reaction was used for mutation analysis. Genetic counseling was provided to members of this family. There were already 3 marriages between the carrier members and 1 between a carrier and noncarrier in this large family; 12 (44.4%) members were found to carry the mutant gene, representing a very high carrier rate compared to the 5.4% carrier frequency of beta-thalassemia in the general population of Pakistan. The family was counseled for prevention of affected births. The initially reluctant family gradually became cooperative and seriously attended the genetic counseling sessions. Cascade testing is more practical than general population screening in a country with limited health facilities where consanguineous marriages are practiced. This report emphasizes the need of extensive testing within families with index cases to identify the carriers of beta-thalassemia in order to reduce disease occurrence through awareness and genetic counseling.