Muhammad Arif Lodhi - Academia.edu (original) (raw)
Papers by Muhammad Arif Lodhi
Phytochem Lett, 2008
Three new benzylated glycosides, locoracemosides A, B and C (1–3) were isolated from the bark of ... more Three new benzylated glycosides, locoracemosides A, B and C (1–3) were isolated from the bark of the stem of Symplocos racemosa Roxb. Their structures were determined by spectroscopic and chemical evidences. They displayed in vitro inhibitory activity against α-chymotrypsin.Locoracemosides A, B and C, three benzylated glycosides were isolated from the bark of the stem of Symplocos racemosa which displayed inhibitory potential against α-chymotrypsin enzyme.
Frontiers in Endocrinology, 2016
α-Glucosidase (extinction coefficient 3.2.1.20) is a primary carbohydrate metabolizing enzyme tha... more α-Glucosidase (extinction coefficient 3.2.1.20) is a primary carbohydrate metabolizing enzyme that acts on the 1-4 associated α-glucose residues. The inhibition of α-glucosidase slows down the process of carbohydrate digestion and avoids postprandial hyperglycemia, which is a major cause of chronic diabetes-associated complication. This study was designed to evaluate the binding capacity of isolated alkaloids with targeted receptor. For this purpose, the three-dimensional tertiary structure of the α-glucosidase was generated by using the Molecular Operating Environment (MOE). The generated model was then validated by using the RAMPAGE and ERRAT server. The molecular docking of 37 alkaloids along with standard acarbose and miglitol reported as a α-glucosidase inhibitor was performed via MOE-Dock implemented in MOE software to find the binding modes of these inhibitors. The results showed that compound 17 (oriciacridone F) and 24 (O-methylmahanine) demonstrated marked interaction with active residues and were comparable to standard inhibitors. In short, this study provided computational background to the reported α-glucosidase inhibitors and thus further detail studies could lead to novel effective compounds.
Medicinal Chemistry Research, 2012
Lett Drug Des Discov, 2008
Journal of Enzyme Inhibition and Medicinal Chemistry, May 1, 2006
Page 1. ORIGINAL RESEARCH Synthesis and anti-inflammatory activity of some pyrazole derivatives S... more Page 1. ORIGINAL RESEARCH Synthesis and anti-inflammatory activity of some pyrazole derivatives Samir M. El-Moghazy Flora F. Barsoum Hamdy M. Abdel-Rahman Adel A. Marzouk Received: 31 December 2010 / Accepted ...
Genome, Sep 1, 1995
Genetic linkage maps of Vitis (2n = 38) have been constructed from a single interspecific hybrid ... more Genetic linkage maps of Vitis (2n = 38) have been constructed from a single interspecific hybrid grape population (60 seedlings) of 'Cayuga White' × 'Aurore'. The maps were primarily based on 422 RAPD markers but also included 16 RFLP and isozyme markers. These maps had an average distance of 6.1 cM between markers and were developed using a double-pseudotestcross strategy. The 'Cayuga White' map had 214 markers covering 1196 cM and that of 'Aurore' spanned over 1477 cM with 225 markers. The 'Cayuga White' map consisted of 20 linkage groups, whereas 22 linkage groups comprised the 'Aurore' map. The number of groups reduced to 19, as in some instances two or more groups from one parent showed homology with a single group from the other parent on the basis of markers heterozygous in both parents. Each linkage group ranged in size from 14 to 135 cM in 'Aurore' and from 14 to 124 cM in 'Cayuga White'. These maps provide enough coverage of the genome to allow quantitative trait locus analysis and map-based gene cloning.Key words: grape genome, pseudotestcross, RAPD, recombination frequency, DNA content.
![Research paper thumbnail of Convergent synthesis of new N -substituted 2-{[5-(1H -indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides as suitable therapeutic agents](https://attachments.academia-assets.com/73910794/thumbnails/1.jpg)
](Brazilian Journal of Pharmaceutical Sciences, 2015
A series of N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides (8... more A series of N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides (8a-w) was synthesized in three steps. The first step involved the sequential conversion of 2-(1H-indol-3-yl)acetic acid (1) to ester (2) followed by hydrazide (3) formation and finally cyclization in the presence of CS2 and alcoholic KOH yielded 5-(1H-indole-3-yl-methyl)-1,3,4-oxadiazole-2-thiol (4). In the second step, aryl/aralkyl amines (5a-w) were reacted with 2-bromoacetyl bromide (6) in basic medium to yield 2-bromo-N-substituted acetamides (7a-w). In the third step, these electrophiles (7a-w) were reacted with 4 to afford the target compounds (8a-w). Structural elucidation of all the synthesized derivatives was done by 1H-NMR, IR and EI-MS spectral techniques. Moreover, they were screened for antibacterial and hemolytic activity. Enzyme inhibition activity was well supported by molecular docking results, for example, compound 8q exhibited better inhibitory potential against α-gluco...
J Enzym Inhib Med Chem, 2008
Journal- Chemical Society of Pakistan
ABSTRACT
Nursing Research, 2015
Little research demonstrating the association between nurse continuity and patient outcomes exist... more Little research demonstrating the association between nurse continuity and patient outcomes exists despite an intuitive belief that continuity makes a difference in care outcomes. The aim of this study was to examine the association of nurse continuity with the prevention of hospital-acquired pressure ulcers (HAPU). A secondary use of data from the Hands on Automated Nursing Data System (HANDS) was performed for this comparative study. The HANDS is a nursing plan of care data set containing 42,403 episodes documented by 787 nurses, on nine units, in four hospitals and includes nurse staffing and patient characteristics. The HANDS data set resides in a "big data" relational database consisting of 89 tables and 747 columns of data. Via data mining, we created an analytic data set of 840 care episodes, 210 with and 630 without HAPUs, matched by nursing unit, patient age, and patient characteristics. Logistic regression analysis determined the association of nurse continuity and additional nurse-staffing variables on HAPU occurrence. Poor nurse continuity (unit mean continuity index = .21-.42 [1.0 = optimal continuity]) was noted on all nine study units. Nutrition, mobility, perfusion, hydration, and skin problems on admission, as well as patient age, were associated with HAPUs (p < .001). Controlling for patient characteristics, nurse continuity, and the interactions between nurse continuity and other nurse-staffing variables were not significantly associated with HAPU development. Patient characteristics including nutrition, mobility, and perfusion were associated with HAPUs, but nurse continuity was not. We demonstrated a high level of variation in the degree of continuity between patient episodes in the HANDS data, showing that it offers rich potential for future study of nurse continuity and its effect on patient outcomes.
Journal- Chemical Society of Pakistan
The mechanism of inhibition of jack bean and Bacillus pasteurii ureases was investigated by sympl... more The mechanism of inhibition of jack bean and Bacillus pasteurii ureases was investigated by symplocomoside (1) which is a phenolic glycoside of salirepin series and has been isolated from Symplocos racemosa Roxb. Lineweaver-Burk, Dixon plots and their secondary replots showed that 1 was a non-competitive inhibitor of these enzymes. Ki values were found to be 77.60 ± 1.22 µM and 63.47 ± 0.92 µM against jack bean and Bacillus pasteurii ureases respectively.
Completion of the human genome has resulted in a paradigm shift in the way biological queries are... more Completion of the human genome has resulted in a paradigm shift in the way biological queries are addressed today. Things that were considered far-fetched only a few years ago, such as personalized medicines, seem to be within our reach. New informatics and research tools have been devised to handle and analyze the influx of a huge amount of data, such as gene chips and microarrays or newly introduced nanoarrays. These devices are used to monitor biochemical and molecular interactions in a highly parallel and automated fashion. Such applications are also multiplexed on microspheres for diagnostic purposes. Because of the advantages of high throughput and high sensitivity, arrays and microspheres are fast becoming indispensable tools in diagnostics, drug discovery, clinical trials and other areas. Surface chemistry is one of the key factors to consider in successfully making sensitive and robust devices. Surface not only controls the anchoring of biomolecules, but also affects molecu...
No doubt, effective administration and management plays a crucial role in strengthening an organi... more No doubt, effective administration and management plays a crucial role in strengthening an organization, association or institution. Therefore, the study was conducted to compare the contemporary and former district educational management systems at elementary and secondary education department in Khyber Pukhtunkhwa (Pakistan). The main objectives of the study were to compare the contemporary and former district educational management systems in elementary and secondary education of Khyber Pukhtunkhwa, and to find out the proper ways and means for the effective management and administration at district level. All the EDOs, DOs, DDOs, ADOs, principals, headmasters and teachers serving in elementary and secondary education department of Khyber Pukhtunkhwa comprised the population of the study. In order to ensure adequate sample, Comparative Study of the Contemporary and Former Educational Management Systems at Elementary and Secondary Education at District Level in Khyber Pukhtunkhwa ...
Palliative & supportive care, Jan 25, 2015
Objective: Electronic health records (EHRs) may contain infomarkers that identify patients near t... more Objective: Electronic health records (EHRs) may contain infomarkers that identify patients near the end of life for whom it would be appropriate to shift care goals to palliative care. Discovery and use of such infomarkers could be used to conduct effectiveness research that ultimately could help to reduce the monumental cost of caring for the dying. The aim of our study was to identify changes in the plans of care that represent infomarkers, which signal a transition of care goals from nonpalliative care ones to those consistent with palliative care. Method: Using an existing electronic health record database generated during a two-year longitudinal study of nine diverse medical-surgical units from four Midwest hospitals and a known group approach, we evaluated patient care episodes for 901 patients who died (mean age = 74.5 ± 14.6 years). We used ANOVA and Tukey's post-hoc tests to compare patient groups. Results: We identified 11 diagnoses, including Death Anxiety and Anticip...
Letters in Drug Design & Discovery, 2008
Earthquake-Resistant Structures - Design, Assessment and Rehabilitation, 2012
Bioinformation, 2013
NS3/4A protease is an important emerging target for the cure of hepatitis C. There are many inhib... more NS3/4A protease is an important emerging target for the cure of hepatitis C. There are many inhibitors of HCV NS3/4A protease that are passing through the clinical improvement indicating momentous reduction in the viral infection rate of patients. In this study molecular docking via MOE-Dock program was used to evaluate binding interactions of ligands with HCV NS3/4A protease. The docking and experimental results were found in good correlation. The best conformations of ligands were analyzed for binding interactions with the residues of binding cavity of NS3/4A protease. The valuable binding interactions and docking scores were observed for compounds 01,
Phytochem Lett, 2008
Three new benzylated glycosides, locoracemosides A, B and C (1–3) were isolated from the bark of ... more Three new benzylated glycosides, locoracemosides A, B and C (1–3) were isolated from the bark of the stem of Symplocos racemosa Roxb. Their structures were determined by spectroscopic and chemical evidences. They displayed in vitro inhibitory activity against α-chymotrypsin.Locoracemosides A, B and C, three benzylated glycosides were isolated from the bark of the stem of Symplocos racemosa which displayed inhibitory potential against α-chymotrypsin enzyme.
Frontiers in Endocrinology, 2016
α-Glucosidase (extinction coefficient 3.2.1.20) is a primary carbohydrate metabolizing enzyme tha... more α-Glucosidase (extinction coefficient 3.2.1.20) is a primary carbohydrate metabolizing enzyme that acts on the 1-4 associated α-glucose residues. The inhibition of α-glucosidase slows down the process of carbohydrate digestion and avoids postprandial hyperglycemia, which is a major cause of chronic diabetes-associated complication. This study was designed to evaluate the binding capacity of isolated alkaloids with targeted receptor. For this purpose, the three-dimensional tertiary structure of the α-glucosidase was generated by using the Molecular Operating Environment (MOE). The generated model was then validated by using the RAMPAGE and ERRAT server. The molecular docking of 37 alkaloids along with standard acarbose and miglitol reported as a α-glucosidase inhibitor was performed via MOE-Dock implemented in MOE software to find the binding modes of these inhibitors. The results showed that compound 17 (oriciacridone F) and 24 (O-methylmahanine) demonstrated marked interaction with active residues and were comparable to standard inhibitors. In short, this study provided computational background to the reported α-glucosidase inhibitors and thus further detail studies could lead to novel effective compounds.
Medicinal Chemistry Research, 2012
Lett Drug Des Discov, 2008
Journal of Enzyme Inhibition and Medicinal Chemistry, May 1, 2006
Page 1. ORIGINAL RESEARCH Synthesis and anti-inflammatory activity of some pyrazole derivatives S... more Page 1. ORIGINAL RESEARCH Synthesis and anti-inflammatory activity of some pyrazole derivatives Samir M. El-Moghazy Flora F. Barsoum Hamdy M. Abdel-Rahman Adel A. Marzouk Received: 31 December 2010 / Accepted ...
Genome, Sep 1, 1995
Genetic linkage maps of Vitis (2n = 38) have been constructed from a single interspecific hybrid ... more Genetic linkage maps of Vitis (2n = 38) have been constructed from a single interspecific hybrid grape population (60 seedlings) of 'Cayuga White' × 'Aurore'. The maps were primarily based on 422 RAPD markers but also included 16 RFLP and isozyme markers. These maps had an average distance of 6.1 cM between markers and were developed using a double-pseudotestcross strategy. The 'Cayuga White' map had 214 markers covering 1196 cM and that of 'Aurore' spanned over 1477 cM with 225 markers. The 'Cayuga White' map consisted of 20 linkage groups, whereas 22 linkage groups comprised the 'Aurore' map. The number of groups reduced to 19, as in some instances two or more groups from one parent showed homology with a single group from the other parent on the basis of markers heterozygous in both parents. Each linkage group ranged in size from 14 to 135 cM in 'Aurore' and from 14 to 124 cM in 'Cayuga White'. These maps provide enough coverage of the genome to allow quantitative trait locus analysis and map-based gene cloning.Key words: grape genome, pseudotestcross, RAPD, recombination frequency, DNA content.
Brazilian Journal of Pharmaceutical Sciences, 2015
A series of N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides (8... more A series of N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides (8a-w) was synthesized in three steps. The first step involved the sequential conversion of 2-(1H-indol-3-yl)acetic acid (1) to ester (2) followed by hydrazide (3) formation and finally cyclization in the presence of CS2 and alcoholic KOH yielded 5-(1H-indole-3-yl-methyl)-1,3,4-oxadiazole-2-thiol (4). In the second step, aryl/aralkyl amines (5a-w) were reacted with 2-bromoacetyl bromide (6) in basic medium to yield 2-bromo-N-substituted acetamides (7a-w). In the third step, these electrophiles (7a-w) were reacted with 4 to afford the target compounds (8a-w). Structural elucidation of all the synthesized derivatives was done by 1H-NMR, IR and EI-MS spectral techniques. Moreover, they were screened for antibacterial and hemolytic activity. Enzyme inhibition activity was well supported by molecular docking results, for example, compound 8q exhibited better inhibitory potential against α-gluco...
J Enzym Inhib Med Chem, 2008
Journal- Chemical Society of Pakistan
ABSTRACT
Nursing Research, 2015
Little research demonstrating the association between nurse continuity and patient outcomes exist... more Little research demonstrating the association between nurse continuity and patient outcomes exists despite an intuitive belief that continuity makes a difference in care outcomes. The aim of this study was to examine the association of nurse continuity with the prevention of hospital-acquired pressure ulcers (HAPU). A secondary use of data from the Hands on Automated Nursing Data System (HANDS) was performed for this comparative study. The HANDS is a nursing plan of care data set containing 42,403 episodes documented by 787 nurses, on nine units, in four hospitals and includes nurse staffing and patient characteristics. The HANDS data set resides in a "big data" relational database consisting of 89 tables and 747 columns of data. Via data mining, we created an analytic data set of 840 care episodes, 210 with and 630 without HAPUs, matched by nursing unit, patient age, and patient characteristics. Logistic regression analysis determined the association of nurse continuity and additional nurse-staffing variables on HAPU occurrence. Poor nurse continuity (unit mean continuity index = .21-.42 [1.0 = optimal continuity]) was noted on all nine study units. Nutrition, mobility, perfusion, hydration, and skin problems on admission, as well as patient age, were associated with HAPUs (p < .001). Controlling for patient characteristics, nurse continuity, and the interactions between nurse continuity and other nurse-staffing variables were not significantly associated with HAPU development. Patient characteristics including nutrition, mobility, and perfusion were associated with HAPUs, but nurse continuity was not. We demonstrated a high level of variation in the degree of continuity between patient episodes in the HANDS data, showing that it offers rich potential for future study of nurse continuity and its effect on patient outcomes.
Journal- Chemical Society of Pakistan
The mechanism of inhibition of jack bean and Bacillus pasteurii ureases was investigated by sympl... more The mechanism of inhibition of jack bean and Bacillus pasteurii ureases was investigated by symplocomoside (1) which is a phenolic glycoside of salirepin series and has been isolated from Symplocos racemosa Roxb. Lineweaver-Burk, Dixon plots and their secondary replots showed that 1 was a non-competitive inhibitor of these enzymes. Ki values were found to be 77.60 ± 1.22 µM and 63.47 ± 0.92 µM against jack bean and Bacillus pasteurii ureases respectively.
Completion of the human genome has resulted in a paradigm shift in the way biological queries are... more Completion of the human genome has resulted in a paradigm shift in the way biological queries are addressed today. Things that were considered far-fetched only a few years ago, such as personalized medicines, seem to be within our reach. New informatics and research tools have been devised to handle and analyze the influx of a huge amount of data, such as gene chips and microarrays or newly introduced nanoarrays. These devices are used to monitor biochemical and molecular interactions in a highly parallel and automated fashion. Such applications are also multiplexed on microspheres for diagnostic purposes. Because of the advantages of high throughput and high sensitivity, arrays and microspheres are fast becoming indispensable tools in diagnostics, drug discovery, clinical trials and other areas. Surface chemistry is one of the key factors to consider in successfully making sensitive and robust devices. Surface not only controls the anchoring of biomolecules, but also affects molecu...
No doubt, effective administration and management plays a crucial role in strengthening an organi... more No doubt, effective administration and management plays a crucial role in strengthening an organization, association or institution. Therefore, the study was conducted to compare the contemporary and former district educational management systems at elementary and secondary education department in Khyber Pukhtunkhwa (Pakistan). The main objectives of the study were to compare the contemporary and former district educational management systems in elementary and secondary education of Khyber Pukhtunkhwa, and to find out the proper ways and means for the effective management and administration at district level. All the EDOs, DOs, DDOs, ADOs, principals, headmasters and teachers serving in elementary and secondary education department of Khyber Pukhtunkhwa comprised the population of the study. In order to ensure adequate sample, Comparative Study of the Contemporary and Former Educational Management Systems at Elementary and Secondary Education at District Level in Khyber Pukhtunkhwa ...
Palliative & supportive care, Jan 25, 2015
Objective: Electronic health records (EHRs) may contain infomarkers that identify patients near t... more Objective: Electronic health records (EHRs) may contain infomarkers that identify patients near the end of life for whom it would be appropriate to shift care goals to palliative care. Discovery and use of such infomarkers could be used to conduct effectiveness research that ultimately could help to reduce the monumental cost of caring for the dying. The aim of our study was to identify changes in the plans of care that represent infomarkers, which signal a transition of care goals from nonpalliative care ones to those consistent with palliative care. Method: Using an existing electronic health record database generated during a two-year longitudinal study of nine diverse medical-surgical units from four Midwest hospitals and a known group approach, we evaluated patient care episodes for 901 patients who died (mean age = 74.5 ± 14.6 years). We used ANOVA and Tukey's post-hoc tests to compare patient groups. Results: We identified 11 diagnoses, including Death Anxiety and Anticip...
Letters in Drug Design & Discovery, 2008
Earthquake-Resistant Structures - Design, Assessment and Rehabilitation, 2012
Bioinformation, 2013
NS3/4A protease is an important emerging target for the cure of hepatitis C. There are many inhib... more NS3/4A protease is an important emerging target for the cure of hepatitis C. There are many inhibitors of HCV NS3/4A protease that are passing through the clinical improvement indicating momentous reduction in the viral infection rate of patients. In this study molecular docking via MOE-Dock program was used to evaluate binding interactions of ligands with HCV NS3/4A protease. The docking and experimental results were found in good correlation. The best conformations of ligands were analyzed for binding interactions with the residues of binding cavity of NS3/4A protease. The valuable binding interactions and docking scores were observed for compounds 01,