Murat Aral - Academia.edu (original) (raw)

Papers by Murat Aral

Annals of Medical and Health Sciences Research, 2019

Journal of Surgical Research, Mar 1, 2020

BACKGROUND Reactive oxygen species-induced cell injury has been considered to be one of the main ... more BACKGROUND Reactive oxygen species-induced cell injury has been considered to be one of the main etiologic factors in ischemia-reperfusion injury (IRI). As a potential antioxidant agent, epigallocatechin gallate (EGCG) was examined in skeletal muscle of the rats after IRI with or without treatment. MATERIALS AND METHODS Tourniquet application applied to the rats' hind limbs was selected as the appropriate IRI method. Animals were randomly distributed to one of the following groups: (1) sham control + SF (saline) (10 mg/kg/i.p.) (SC-SF), (2) IRI (4 + 2 h) + SF (10 mg/kg/i.p.) (IRI-SF), (3) IRI and EGCG (25 mg/kg/i.p.) (IRI-EG25), and (4) IRI and EGCG (50 mg/kg/i.p) (IRI-EG50). In another set of experiments with identical groups, the only difference was that the reperfusion period was 24 h. A number of different parameters relating to the damage seen in the skeletal muscles, lungs, kidneys, and liver and particular cytokines were measured by proper analytical methods. RESULTS In comparison with the SC-SF group, IRI (4 + 2 h) induced an increase in the total oxidative status of skeletal muscle (10.17 ± 0.61 versus 15.74 ± 1.10) and blood creatine phosphokinase (CPK) (669.88 ± 50.23 versus 7202.38 ± 766.13) and lactate dehydrogenase levels (686.00 ± 67.48 versus 1343.00 ± 113.01). Although 25 mg/kg EGCG could not reverse these parameters to their normal levels, the higher dose of EGCG, that is, 50 mg/kg, was sufficient to prevent the increases seen in total oxidative status (8.55 ± 0.85) and CPK levels (4741.63 ± 339.40). In addition, reduced total antioxidant status of skeletal muscle in the IRI-SF group (0.50 ± 0.06) was elevated by the administration of EGCG (50 mg/kg) (0.85 ± 0.04). Regarding remote organ injury, only alanine transaminase (ALT) and aspartate transaminase (AST) levels were found to be increased, showing a slight damage in liver tissue. However, neither dose of EGCG was able to prevent this deleterious effect. As for cytokines (interleukin-1β, IL-6, IL-8, tumor necrosis factor-α, and monocyte chemotactic protein-1), there were no differences between the study groups. In regard to long-term IRI (i.e., 4 + 24 h), statistically significantly elevated parameters in the IRI-SF group were as follows: CPK, lactate dehydrogenase, creatinine (Cr), and blood urea nitrogen. On the other hand, none of them were influenced by either dose of EGCG. According to the results, EGCG demonstrates a considerable protective effect toward IRI (4 + 2 h) of skeletal muscle. CONCLUSIONS Although oxidative stress seems to play a significant role both in the pathogenesis of IRI and in the mechanism of action of EGCG, there is no evidence that inflammatory cytokines are, at least in our model, crucial mediators regarding the former events.

Flora infeksiyon hastalıkları ve klinik mikrobiyoloji dergisi, 2017

Sakarya Medical Journal

Amaç: Bu çalışmada, 2020 yılı içerisinde hastanemizin yoğun bakım üniteleri ve yataklı servisleri... more Amaç: Bu çalışmada, 2020 yılı içerisinde hastanemizin yoğun bakım üniteleri ve yataklı servislerinden mikrobiyoloji laboratuvarına gönderilen çeşitli kültür örneklerinden izole edilen gram negatif bakteriler ve antibiyotik dirençlerinin retrospektif olarak değerlendirilmesi amaçlanmıştır. Metod: Ocak 2020-Aralık 2020 tarihleri arasında yoğun bakım üniteleri ve yataklı servislerden mikrobiyoloji laboratuvarına gönderilen çeşitli hasta örneklerinden sık izole edilen Gram negatif bakteriler çalışmaya dahil edilmiştir. Bakteri tanımlanması ve antibiyotik duyarlılık testleri konvansiyonel yöntemler ve otomatize sistemler kullanılarak yapılmıştır Bulgular: Bir yıllık süreçte yoğun bakım ünitelerindeki hastalardan sırasıyla %49.7’u endotrakeal aspirat kültürlerinden izole edilen 761 Acinetobacter baumanii, %51.7’si idrar kültüründen izole edilen 478 E. coli, %31.2’si endotrakeal aspirat kültürlerinden izole edilen 417 Klebsiella pneumoniae, %51.5’i endotrakeal aspirat kültürlerinden izole ...

ANKEM dergisi, Nov 30, 2012

Sonuç olarak P.aeruginosa suşları en düşük direnci aminoglikozidlere göstermiştir. P.aeruginosa s... more Sonuç olarak P.aeruginosa suşları en düşük direnci aminoglikozidlere göstermiştir. P.aeruginosa suşlarının neden olduğu infeksiyonların tedavisinde aminoglikozidler halen tercih edilen antibiyotikler olmasına rağmen, bu grup ve diğer grup antibiyotiklerin büyük çoğunluğuna karşı artan direnç oranlarına dikkat edilmelidir. Bu nedenle rasyonel olmayan ilaç kullanımını önlemek için alınan önlemler dikkatle incelenmeli ve direnç gelişimi düzenli olarak takip edilmelidir.

Türk hijiyen ve deneysel biyoloji dergisi, 2011

Method: Sheep blood agar and eosin methylene-blue (EMB) agar were used for culturing. At the end ... more Method: Sheep blood agar and eosin methylene-blue (EMB) agar were used for culturing. At the end of 18-24 hours incubation at 37°C, Gram-positive cocci colonies with negative catalase test and positive PYR test were obtained. Species-level identification of these strains, were identified by VITEK 2 (bioMerieux, France) method. The antibiotic susceptibility of isolated strains are defined according to of Clinical and Laboratory Standards Institute (CLSI) standards. Multiple isolates from the same patient has not been tested. Results: Total of 158 strains were isolated and, 96 of them were E.faecium and 62 were E.faecalis. E.faecalis and E.faecium strains isolated from different samples have got similar rates which were very close to each other.

ANKEM Dergisi, 2010

... ARAŞTIRILMASI Murat ARAL, Serpil DOĞAN, Nuriye İsmihan Ece PAKÖZ ... Gülhan ve ark(12)'n... more ... ARAŞTIRILMASI Murat ARAL, Serpil DOĞAN, Nuriye İsmihan Ece PAKÖZ ... Gülhan ve ark(12)'nın yaptıkları çalışmada, 2004 ve 2006 yıl-ları arasındaki antibiyotik direnç değişimleri göz önüne alındığında meropenem ve siproflok-sasin için istatistiksel olarak anlamlı direnç artışı ...

Sakarya Medical Journal, 2017

Amaç Bu çalımada HBV DNA ve HCV RNA'sı pozitif olan hastalarda, Anti-nükleer antikor (ANA), Anti-... more Amaç Bu çalımada HBV DNA ve HCV RNA'sı pozitif olan hastalarda, Anti-nükleer antikor (ANA), Anti-Double Stranded DNA (dsDNA), Anti-single stranded DNA (ssDNA), Anti-SM Antikor, Anti-tiroglobulin Antikor(a-TG), Anti-düz kas antikor(ASMA), Anti-mitokondriyal antikor M2(AMA-M2), Karaciğer böbrek mikrozomal antikor-1 (LKM-1) otoantikorlarının seroprevelansını saptamak ve değerlerin kendi aralarında ve normal populasyon değerleriyle karşılaştırılması planlanmıştır. Gereç ve Yöntemler: 30 HBV DNA'sı pozitif, 30 HCV RNA 'sı pozitif Kronik hepatit B ve C tanılı hasta serumları ve 30 sağlıklı kontrol grubu serum örnekleri kullanılarak yapılmıştır.Çalışma sırasında serumlar oda sıcaklığında eritilmesinin ardından ELİSA yöntemi ile çalışılmıştır. Bulgular: Hepatit B hasta grubunda 3 olguda ANA(%10), 10 olguda ASMA (%33.3), 1 olguda LKM-1(%3.3), 16 olguda AMA-M2(%53.3),1 olguda anti-TG(%3.3), 3 olguda anti-ds DNA(%10), 3 olguda anti-ss DNA(%10) antikor pozitifliği olmak üzere 20 HBV-DNA pozitif hastada toplam 37 otoantikor varlığı belirlenmiştir. Kronik hapatit C grubunda, 4 olguda ANA (%13.3), 14 olguda ASMA (46.7), 2 olguda LKM-1(%6.7), 12 olguda AMA-M2 (%40), 5 olguda anti-TG(%16.7), 4 olguda anti-ds DNA (%13.3), 4 olguda anti-ss DNA (%13.3) antikor pozitifliği olmak üzere 23 HCV-RNA pozitif hastada toplam 45 otoantikor varlığı belirlenmiştir. Kontrol grubunda ise 2 olguda ANA (%6.7), 5 olguda ASMA(%16.7), 8 olguda AMA-M2(%26.7), 3 olguda anti-TG(%10), 2 olguda anti-ds DNA (%6.7), 2 olguda anti-ss DNA (%6.7), antikor pozitifliği olmak üzere 14 sağlıklı hastada toplam 22 otoantikor varlığı belirlenmiştir.

KSÜ Tıp Fakültesi dergisi, 2005

KSÜ Tıp Fakültesi dergisi, Mar 21, 2022

Objective: The aim of this study is to observe the current situation in the hospital medical micr... more Objective: The aim of this study is to observe the current situation in the hospital medical microbiology laboratory, to determine the waste situations, to evaluate the applicability of lean production systems in the hospital medical microbiology laboratory, to provide training on lean management, to determine the points that can be improved with the use of these techniques and to present improvement suggestions. Materials and Methods: In the medical microbiology laboratory of our hospital; value flow mapping technique for improvements in the bacteriology unit, the 5S technique for improvements in the laboratory warehouse area, the fishbone technique for the improvement of warehouse-stock controls, and the Kaizen technique while improving the barcode problems of the sample tubes. Results: When the results were examined in blood culture, the time of 2094 minutes in the blood culture device of the samples was reduced to 1242 minutes. Even if the targeted time could not be reached, 47.4% of the target was achieved and the improvement was achieved as 40.6%. Mandatory 63 steps for samples in blood culture tests were reduced to 56 steps, saving 7 steps (11%) for each sample. It is ensured that the stock on the laboratory system and the real stock are the same. The laboratory warehouse and working areas have been arranged and the working environment has been made clean and tidy. The barcodes were returned to the purchased company and replaced with new barcodes that adhere better to the tube and do not cause these problems. Conclusions: Institutions in the health sector need to use resources better and provide more efficient service by evaluating them with a lean perspective. It has been seen with our study that more effective results can be obtained by planning a longer and more comprehensive study.

Sakarya tıp dergisi, Sep 30, 2017

Amaç Bu çalışmada, S.aureus izolatlarının metisilin, yüksek düzey mupirosin ve fusidik asite karş... more Amaç Bu çalışmada, S.aureus izolatlarının metisilin, yüksek düzey mupirosin ve fusidik asite karşı antibiyotik direnç özelliklerinin fenotipik ve genotipik olarak açığa çıkarılması amaçlanmıştır. Gereç ve Yöntemler: Antibiyotik direnç özelliği bilinmeyen 100 farklı Staphylococcus.aureus (S.aureus) izolatı klinik örneklerden saflaştırılmış ve bu izolatların metisilin, yüksek düzey mupirosin ve fusidik asit antbiyotik direnci ve plazmit içerikleri belirlenmiştir. Metisilin, yüksek düzey mupirosin ve fusidik asit duyarlılıkları phoenixsistemi ile belirlenmiştir. Ayrıca Metisilin direnç özelliği 30 µg sefoksitin diski kullanılarak disk difüzyon yöntemi ile de araştırılmıştır. İzolatlardaki antibiyotik direnç özelliği ile ilgili mecA, ileS-2, fusA, fusB ve fusC genlerinin varlığı ise PCR amplifikasyonu ile araştırılmıştır. Bulgular: Disk difüzyon ve phoenix analiz sonuçlarına göre tanımlanan S. aureus izolatlarınının sefoksitin direnci %19 olarak tespit edilmiştir. Phoenix otomotize sistem ile yüksek düzey mupirosin ve fusidik asit direnci tespit edilmemiştir. Moleküler analiz sonuçlarına göre ise mecA, fusA ve fusC genlerinin izolatlardaki frekansı sırası ile %19, %98 ve %1 olarak belirlenmiş fakat ileS-2 ve fusB genleri amplifiye edilmemiştir. Antibiyotik direnç ve plazmit varlığı arasındaki ilişkinin belirlenebilmesi için izolatların plazmit varlığı belirlenmiş ve izolatların %79'unda farklı sayılarda plazmit içerdiği tespit edilmiştir. Sonuç: Fenotipik ve genotipik yöntemle elde edilenlere sonuçlar benzerlik göstermesine karşın benzer olmayan bazı sonuçları da içermektedir. Stafilokok enfeksiyonlarının tedavisinde, antibiyotik direnç genlerinin doğru ve hızlı bir şekilde teşhisi, enfeksiyonların yayılmasının önlenmesinde çok önemlidir. Antibiyotik dirençinin doğru saptanması için fenotipik yöntemler ile birlikte PCR bazlı moleküler yöntemler de tercih edilmelidir.

PubMed, Sep 1, 2004

Aim of the study: To investigate the pathogenicity and antibiotic resistance of coagulase-negativ... more Aim of the study: To investigate the pathogenicity and antibiotic resistance of coagulase-negative staphylococci (CNS) isolated from the maxillary and ethmoid sinuses of patients undergoing endoscopic sinus surgery for chronic sinusitis. Patients and methods: Ninety-three patients (63 males, 30 females) aged between 19 - 68 years, who had undergone functional endoscopic sinus surgery (FESS) for chronic sinusitis, were included in the study. Nasal mucosa, skin and adjacent structures were cleansed with povidone-iodine solution before surgery to prevent a probable contamination. In all patients, nasal swabs were taken before and after the application of povidone-iodine solution. Colonies isolated and identified as Staphylococci in cultures were further investigated for pathogenicity and antibiotic susceptibility. Slime test was used to determine the pathogenicity of CNS. The relationship between antibiotic resistance of pathogenic and non-pathogenic CNS was compared by chi2 analysis. Results: While bacterial growth rate was 62.3% in nasal swab cultures taken before the application of povidone-iodine solution, it decreased to 12.9% after the application of solution. Microorganisms were isolated in 95.6% of cultures taken from maxillary sinuses and in 91.3% of cultures obtained from ethmoid sinuses during the FESS. The most frequently isolated microorganism in each of the sinuses was CNS. Slime test was carried out in 30 CNS isolated. Twelve of these were slime positive and 18 were slime negative. While 83.3% of CNS isolated was resistant to penicilin, all of CNS were sensitive to vancomycin and teikoplanine. The difference between slime positive and slime negative CNS for gentamicin and ciprofloxacin resistance was statistically significant (p<0.05). Conclusion: We consider that the pathogenicity tests like slime production and antimicrobial susceptibilities of CNS frequently isolated from the patients with chronic sinusitis should be investigated and also these microorganisms should be kept in mind in the selection of empiric treatment.

European Journal of Therapeutics

The aim of this study is to find out the HBV seroprevalance in the people admitted to Kahramanmar... more The aim of this study is to find out the HBV seroprevalance in the people admitted to Kahramanmaraş Sütçü İmam University Medical Faculty Check- up clinics. Demographic features and HBV vaccine status of 377 cases who admitted to the check-up clinics of Kahramanmaraş Sütçü İmam University Medical Faculty Hospital, were questioned and the blood samples of each patient were collected. Serum HbsAg and Anti-HBs parameters were evaluated by Access IEIA (Immunoenzymatic ""sandwich"" Immunoassay) method. The cases with positive HbsAg were encountered as HBV carrier. 221 of the cases (58.6%) who enrolled in the study were female, 156 (41.4%) were male. Mean age was 39.2±12.5. Three hundred and two (96.1%) were having social insurance coverage. Numbers of the known hepatitis B carrier as HbsAg positive were 16 (4.2%) and immunized (Anti-HBs positive) ones were 50 (13.3%). Twelve cases (3.2%) had the history of hepatitis B vaccination. All the cases who had vaccination wer...

Türk Mikrobiyoloji Cemiyeti Dergisi

Tez (Yüksek Lisans) -- İstanbul Teknik Üniversitesi, Fen Bilimleri Enstitüsü, 2006Thesis (M.Sc.) ... more Tez (Yüksek Lisans) -- İstanbul Teknik Üniversitesi, Fen Bilimleri Enstitüsü, 2006Thesis (M.Sc.) -- İstanbul Technical University, Institute of Science and Technology, 2006Bu çalışmada, çelik liflerin mekanik özellikleri ve kompozitlerin özelliklerini incelemek için kancalı ve/veya kancasız 3 farklı çelik tel kullanıldı. Her çelik lif hacim oranı değişkendi fakat toplam hacim % 2’de sabit tutuldu. Kısa kancasız düz çelik lifler,yüksek dayanımlı tunç kaplı olup, 6 mm. boyunda ve 0,16 mm. çapındadır. Normal dayanımlı çelik liflerin narinlikleri 55 ve 65 dir ve dayanımları aynıdır (1150 MPa). Düz kesimli çelik liflerin çekme dayanımları 2250 MPa dır. Farklı lifli toplam lifli ve lifsiz 12 beton karışımı üretildi. Karma lifli ve/veya lifsiz çelik tel içeren betonların basınç dayanımları, elastisite modülleri, net eğilme dayanımları, yarmada çekme dayanımları ve kırılma enerjileri yalın betonunkilerle karşılaştırıldı. Kısa liflerin,mikro çatlakların önlemesinde köprü görevi gördüğü ve bu...

Viral Hepatitis Journal

Objectives: Chronic hepatitis C virus (HCV) infection still continues to be a significant health ... more Objectives: Chronic hepatitis C virus (HCV) infection still continues to be a significant health problem in the entire world. In addition to this, knowing about the existing genotypes in a region is highly important in terms of guiding antiviral treatment and understanding the epidemiology in that region. In our study, we examined the varying genotype distribution in our region and affecting factors. Materials and Methods: To determine the HCV genotype distribution in Kahramanmaraş in Turkey, patients who were determined to be HCV-positive in the last 4.5 years and whose genotypes were studied retrospectively searched from records and included in the study. Results: Genotype 1 was the most prevalent genotype (47%) in Kahramanmaraş. The second most prevalent (45%) genotype was genotype 3. Additionally, genotypes 2 and 4 were seen at the rates of respectively 2% and 6%. While there was male dominance in genotypes 1, 2, and 3, genotype 4 had female dominance (69%). There was a very high male dominance in genotype 3 (95%), and the mean age of the patients was 26.4. Conclusion: The epidemiology of HCV may show serious variations at locations that receive intense migration and where increased drug usage is observed. The main point in preventing HCV infection should be the identification and elimination of risk factors.

KSÜ Tıp Fakültesi dergisi, 2005

Türk mikrobiyoloji cemiyeti dergisi, 2017

Eastern Journal Of Medicine, 2019

In the patients who have perioperative renal failure risk, anesthetical substances should be choo... more In the patients who have perioperative renal failure risk, anesthetical substances should be choosen with caution to protect the function of kidneys. Ketamine, an anesthetic induction agent, is generally used in patients with severe hypotension or respiratory depression. We aimed to evaluate the different doses of ketamine's effects on ischemia/reperfusion (I/R) damage mediated by free radicals in rats. Materials and methods: In this study, 42 Wistar albino male rats were splitted randomly into 7 different groups. In the ketamine group, ketamine was applied intraperitoneally (IP) in different doses (3 mg kg‫-‬ 1 , 10 mg kg‫-‬ 1 , 30 mg kg‫-‬ 1 , 60 mg kg‫-‬ 1 , 80 mg kg‫-‬ 1) on the 45 th minutes. Clamps were opened at the end of 60 minutes ischemia period. At the end of the reperfusion period, renal tissue and blood sample s were taken from the rats. In the plasma samples, pro-inflammatory biomarkers [Interleukin (IL)-1β, IL-6, tumour necrosis factor alpha (TNF-α)] were analysed. In renal tissue samples, antioxidating activities [Superoxide dismutase (SOD), glutathione perox idase (GPx) and nitric oxide (NO)] and lipid peroxidation product "Malondialdehyde (MDA)" levels were studied biochemically. Renal tissue damage was evaluated histopathologically. There were no differences among the beneficial effects of ketamine given gro ups (10-30-60-80 mg kg‫-‬ 1 doses) before reperfusion in the way of antioxidant activities, pro-inflammatory markers and lipid peroxidation product. When ketamin was applied in 3 mg kg‫-‬ 1 there were beneficial effects on tissues in the way of SOD, GPx, NO, MDA values and histopathologically (p<0.05). Some studies have shown that ketamine has little anti-inflammatory properties. This animal study has shown that ketamine in low doses significantly reduces the I/R injury in rats (p<0.05).

Annals of Medical and Health Sciences Research, 2019

Journal of Surgical Research, Mar 1, 2020

BACKGROUND Reactive oxygen species-induced cell injury has been considered to be one of the main ... more BACKGROUND Reactive oxygen species-induced cell injury has been considered to be one of the main etiologic factors in ischemia-reperfusion injury (IRI). As a potential antioxidant agent, epigallocatechin gallate (EGCG) was examined in skeletal muscle of the rats after IRI with or without treatment. MATERIALS AND METHODS Tourniquet application applied to the rats' hind limbs was selected as the appropriate IRI method. Animals were randomly distributed to one of the following groups: (1) sham control + SF (saline) (10 mg/kg/i.p.) (SC-SF), (2) IRI (4 + 2 h) + SF (10 mg/kg/i.p.) (IRI-SF), (3) IRI and EGCG (25 mg/kg/i.p.) (IRI-EG25), and (4) IRI and EGCG (50 mg/kg/i.p) (IRI-EG50). In another set of experiments with identical groups, the only difference was that the reperfusion period was 24 h. A number of different parameters relating to the damage seen in the skeletal muscles, lungs, kidneys, and liver and particular cytokines were measured by proper analytical methods. RESULTS In comparison with the SC-SF group, IRI (4 + 2 h) induced an increase in the total oxidative status of skeletal muscle (10.17 ± 0.61 versus 15.74 ± 1.10) and blood creatine phosphokinase (CPK) (669.88 ± 50.23 versus 7202.38 ± 766.13) and lactate dehydrogenase levels (686.00 ± 67.48 versus 1343.00 ± 113.01). Although 25 mg/kg EGCG could not reverse these parameters to their normal levels, the higher dose of EGCG, that is, 50 mg/kg, was sufficient to prevent the increases seen in total oxidative status (8.55 ± 0.85) and CPK levels (4741.63 ± 339.40). In addition, reduced total antioxidant status of skeletal muscle in the IRI-SF group (0.50 ± 0.06) was elevated by the administration of EGCG (50 mg/kg) (0.85 ± 0.04). Regarding remote organ injury, only alanine transaminase (ALT) and aspartate transaminase (AST) levels were found to be increased, showing a slight damage in liver tissue. However, neither dose of EGCG was able to prevent this deleterious effect. As for cytokines (interleukin-1β, IL-6, IL-8, tumor necrosis factor-α, and monocyte chemotactic protein-1), there were no differences between the study groups. In regard to long-term IRI (i.e., 4 + 24 h), statistically significantly elevated parameters in the IRI-SF group were as follows: CPK, lactate dehydrogenase, creatinine (Cr), and blood urea nitrogen. On the other hand, none of them were influenced by either dose of EGCG. According to the results, EGCG demonstrates a considerable protective effect toward IRI (4 + 2 h) of skeletal muscle. CONCLUSIONS Although oxidative stress seems to play a significant role both in the pathogenesis of IRI and in the mechanism of action of EGCG, there is no evidence that inflammatory cytokines are, at least in our model, crucial mediators regarding the former events.

Flora infeksiyon hastalıkları ve klinik mikrobiyoloji dergisi, 2017

Sakarya Medical Journal

Amaç: Bu çalışmada, 2020 yılı içerisinde hastanemizin yoğun bakım üniteleri ve yataklı servisleri... more Amaç: Bu çalışmada, 2020 yılı içerisinde hastanemizin yoğun bakım üniteleri ve yataklı servislerinden mikrobiyoloji laboratuvarına gönderilen çeşitli kültür örneklerinden izole edilen gram negatif bakteriler ve antibiyotik dirençlerinin retrospektif olarak değerlendirilmesi amaçlanmıştır. Metod: Ocak 2020-Aralık 2020 tarihleri arasında yoğun bakım üniteleri ve yataklı servislerden mikrobiyoloji laboratuvarına gönderilen çeşitli hasta örneklerinden sık izole edilen Gram negatif bakteriler çalışmaya dahil edilmiştir. Bakteri tanımlanması ve antibiyotik duyarlılık testleri konvansiyonel yöntemler ve otomatize sistemler kullanılarak yapılmıştır Bulgular: Bir yıllık süreçte yoğun bakım ünitelerindeki hastalardan sırasıyla %49.7’u endotrakeal aspirat kültürlerinden izole edilen 761 Acinetobacter baumanii, %51.7’si idrar kültüründen izole edilen 478 E. coli, %31.2’si endotrakeal aspirat kültürlerinden izole edilen 417 Klebsiella pneumoniae, %51.5’i endotrakeal aspirat kültürlerinden izole ...

ANKEM dergisi, Nov 30, 2012

Sonuç olarak P.aeruginosa suşları en düşük direnci aminoglikozidlere göstermiştir. P.aeruginosa s... more Sonuç olarak P.aeruginosa suşları en düşük direnci aminoglikozidlere göstermiştir. P.aeruginosa suşlarının neden olduğu infeksiyonların tedavisinde aminoglikozidler halen tercih edilen antibiyotikler olmasına rağmen, bu grup ve diğer grup antibiyotiklerin büyük çoğunluğuna karşı artan direnç oranlarına dikkat edilmelidir. Bu nedenle rasyonel olmayan ilaç kullanımını önlemek için alınan önlemler dikkatle incelenmeli ve direnç gelişimi düzenli olarak takip edilmelidir.

Türk hijiyen ve deneysel biyoloji dergisi, 2011

Method: Sheep blood agar and eosin methylene-blue (EMB) agar were used for culturing. At the end ... more Method: Sheep blood agar and eosin methylene-blue (EMB) agar were used for culturing. At the end of 18-24 hours incubation at 37°C, Gram-positive cocci colonies with negative catalase test and positive PYR test were obtained. Species-level identification of these strains, were identified by VITEK 2 (bioMerieux, France) method. The antibiotic susceptibility of isolated strains are defined according to of Clinical and Laboratory Standards Institute (CLSI) standards. Multiple isolates from the same patient has not been tested. Results: Total of 158 strains were isolated and, 96 of them were E.faecium and 62 were E.faecalis. E.faecalis and E.faecium strains isolated from different samples have got similar rates which were very close to each other.

ANKEM Dergisi, 2010

... ARAŞTIRILMASI Murat ARAL, Serpil DOĞAN, Nuriye İsmihan Ece PAKÖZ ... Gülhan ve ark(12)'n... more ... ARAŞTIRILMASI Murat ARAL, Serpil DOĞAN, Nuriye İsmihan Ece PAKÖZ ... Gülhan ve ark(12)'nın yaptıkları çalışmada, 2004 ve 2006 yıl-ları arasındaki antibiyotik direnç değişimleri göz önüne alındığında meropenem ve siproflok-sasin için istatistiksel olarak anlamlı direnç artışı ...

Sakarya Medical Journal, 2017

Amaç Bu çalımada HBV DNA ve HCV RNA'sı pozitif olan hastalarda, Anti-nükleer antikor (ANA), Anti-... more Amaç Bu çalımada HBV DNA ve HCV RNA'sı pozitif olan hastalarda, Anti-nükleer antikor (ANA), Anti-Double Stranded DNA (dsDNA), Anti-single stranded DNA (ssDNA), Anti-SM Antikor, Anti-tiroglobulin Antikor(a-TG), Anti-düz kas antikor(ASMA), Anti-mitokondriyal antikor M2(AMA-M2), Karaciğer böbrek mikrozomal antikor-1 (LKM-1) otoantikorlarının seroprevelansını saptamak ve değerlerin kendi aralarında ve normal populasyon değerleriyle karşılaştırılması planlanmıştır. Gereç ve Yöntemler: 30 HBV DNA'sı pozitif, 30 HCV RNA 'sı pozitif Kronik hepatit B ve C tanılı hasta serumları ve 30 sağlıklı kontrol grubu serum örnekleri kullanılarak yapılmıştır.Çalışma sırasında serumlar oda sıcaklığında eritilmesinin ardından ELİSA yöntemi ile çalışılmıştır. Bulgular: Hepatit B hasta grubunda 3 olguda ANA(%10), 10 olguda ASMA (%33.3), 1 olguda LKM-1(%3.3), 16 olguda AMA-M2(%53.3),1 olguda anti-TG(%3.3), 3 olguda anti-ds DNA(%10), 3 olguda anti-ss DNA(%10) antikor pozitifliği olmak üzere 20 HBV-DNA pozitif hastada toplam 37 otoantikor varlığı belirlenmiştir. Kronik hapatit C grubunda, 4 olguda ANA (%13.3), 14 olguda ASMA (46.7), 2 olguda LKM-1(%6.7), 12 olguda AMA-M2 (%40), 5 olguda anti-TG(%16.7), 4 olguda anti-ds DNA (%13.3), 4 olguda anti-ss DNA (%13.3) antikor pozitifliği olmak üzere 23 HCV-RNA pozitif hastada toplam 45 otoantikor varlığı belirlenmiştir. Kontrol grubunda ise 2 olguda ANA (%6.7), 5 olguda ASMA(%16.7), 8 olguda AMA-M2(%26.7), 3 olguda anti-TG(%10), 2 olguda anti-ds DNA (%6.7), 2 olguda anti-ss DNA (%6.7), antikor pozitifliği olmak üzere 14 sağlıklı hastada toplam 22 otoantikor varlığı belirlenmiştir.

KSÜ Tıp Fakültesi dergisi, 2005

KSÜ Tıp Fakültesi dergisi, Mar 21, 2022

Objective: The aim of this study is to observe the current situation in the hospital medical micr... more Objective: The aim of this study is to observe the current situation in the hospital medical microbiology laboratory, to determine the waste situations, to evaluate the applicability of lean production systems in the hospital medical microbiology laboratory, to provide training on lean management, to determine the points that can be improved with the use of these techniques and to present improvement suggestions. Materials and Methods: In the medical microbiology laboratory of our hospital; value flow mapping technique for improvements in the bacteriology unit, the 5S technique for improvements in the laboratory warehouse area, the fishbone technique for the improvement of warehouse-stock controls, and the Kaizen technique while improving the barcode problems of the sample tubes. Results: When the results were examined in blood culture, the time of 2094 minutes in the blood culture device of the samples was reduced to 1242 minutes. Even if the targeted time could not be reached, 47.4% of the target was achieved and the improvement was achieved as 40.6%. Mandatory 63 steps for samples in blood culture tests were reduced to 56 steps, saving 7 steps (11%) for each sample. It is ensured that the stock on the laboratory system and the real stock are the same. The laboratory warehouse and working areas have been arranged and the working environment has been made clean and tidy. The barcodes were returned to the purchased company and replaced with new barcodes that adhere better to the tube and do not cause these problems. Conclusions: Institutions in the health sector need to use resources better and provide more efficient service by evaluating them with a lean perspective. It has been seen with our study that more effective results can be obtained by planning a longer and more comprehensive study.

Sakarya tıp dergisi, Sep 30, 2017

Amaç Bu çalışmada, S.aureus izolatlarının metisilin, yüksek düzey mupirosin ve fusidik asite karş... more Amaç Bu çalışmada, S.aureus izolatlarının metisilin, yüksek düzey mupirosin ve fusidik asite karşı antibiyotik direnç özelliklerinin fenotipik ve genotipik olarak açığa çıkarılması amaçlanmıştır. Gereç ve Yöntemler: Antibiyotik direnç özelliği bilinmeyen 100 farklı Staphylococcus.aureus (S.aureus) izolatı klinik örneklerden saflaştırılmış ve bu izolatların metisilin, yüksek düzey mupirosin ve fusidik asit antbiyotik direnci ve plazmit içerikleri belirlenmiştir. Metisilin, yüksek düzey mupirosin ve fusidik asit duyarlılıkları phoenixsistemi ile belirlenmiştir. Ayrıca Metisilin direnç özelliği 30 µg sefoksitin diski kullanılarak disk difüzyon yöntemi ile de araştırılmıştır. İzolatlardaki antibiyotik direnç özelliği ile ilgili mecA, ileS-2, fusA, fusB ve fusC genlerinin varlığı ise PCR amplifikasyonu ile araştırılmıştır. Bulgular: Disk difüzyon ve phoenix analiz sonuçlarına göre tanımlanan S. aureus izolatlarınının sefoksitin direnci %19 olarak tespit edilmiştir. Phoenix otomotize sistem ile yüksek düzey mupirosin ve fusidik asit direnci tespit edilmemiştir. Moleküler analiz sonuçlarına göre ise mecA, fusA ve fusC genlerinin izolatlardaki frekansı sırası ile %19, %98 ve %1 olarak belirlenmiş fakat ileS-2 ve fusB genleri amplifiye edilmemiştir. Antibiyotik direnç ve plazmit varlığı arasındaki ilişkinin belirlenebilmesi için izolatların plazmit varlığı belirlenmiş ve izolatların %79'unda farklı sayılarda plazmit içerdiği tespit edilmiştir. Sonuç: Fenotipik ve genotipik yöntemle elde edilenlere sonuçlar benzerlik göstermesine karşın benzer olmayan bazı sonuçları da içermektedir. Stafilokok enfeksiyonlarının tedavisinde, antibiyotik direnç genlerinin doğru ve hızlı bir şekilde teşhisi, enfeksiyonların yayılmasının önlenmesinde çok önemlidir. Antibiyotik dirençinin doğru saptanması için fenotipik yöntemler ile birlikte PCR bazlı moleküler yöntemler de tercih edilmelidir.

PubMed, Sep 1, 2004

Aim of the study: To investigate the pathogenicity and antibiotic resistance of coagulase-negativ... more Aim of the study: To investigate the pathogenicity and antibiotic resistance of coagulase-negative staphylococci (CNS) isolated from the maxillary and ethmoid sinuses of patients undergoing endoscopic sinus surgery for chronic sinusitis. Patients and methods: Ninety-three patients (63 males, 30 females) aged between 19 - 68 years, who had undergone functional endoscopic sinus surgery (FESS) for chronic sinusitis, were included in the study. Nasal mucosa, skin and adjacent structures were cleansed with povidone-iodine solution before surgery to prevent a probable contamination. In all patients, nasal swabs were taken before and after the application of povidone-iodine solution. Colonies isolated and identified as Staphylococci in cultures were further investigated for pathogenicity and antibiotic susceptibility. Slime test was used to determine the pathogenicity of CNS. The relationship between antibiotic resistance of pathogenic and non-pathogenic CNS was compared by chi2 analysis. Results: While bacterial growth rate was 62.3% in nasal swab cultures taken before the application of povidone-iodine solution, it decreased to 12.9% after the application of solution. Microorganisms were isolated in 95.6% of cultures taken from maxillary sinuses and in 91.3% of cultures obtained from ethmoid sinuses during the FESS. The most frequently isolated microorganism in each of the sinuses was CNS. Slime test was carried out in 30 CNS isolated. Twelve of these were slime positive and 18 were slime negative. While 83.3% of CNS isolated was resistant to penicilin, all of CNS were sensitive to vancomycin and teikoplanine. The difference between slime positive and slime negative CNS for gentamicin and ciprofloxacin resistance was statistically significant (p<0.05). Conclusion: We consider that the pathogenicity tests like slime production and antimicrobial susceptibilities of CNS frequently isolated from the patients with chronic sinusitis should be investigated and also these microorganisms should be kept in mind in the selection of empiric treatment.

European Journal of Therapeutics

The aim of this study is to find out the HBV seroprevalance in the people admitted to Kahramanmar... more The aim of this study is to find out the HBV seroprevalance in the people admitted to Kahramanmaraş Sütçü İmam University Medical Faculty Check- up clinics. Demographic features and HBV vaccine status of 377 cases who admitted to the check-up clinics of Kahramanmaraş Sütçü İmam University Medical Faculty Hospital, were questioned and the blood samples of each patient were collected. Serum HbsAg and Anti-HBs parameters were evaluated by Access IEIA (Immunoenzymatic ""sandwich"" Immunoassay) method. The cases with positive HbsAg were encountered as HBV carrier. 221 of the cases (58.6%) who enrolled in the study were female, 156 (41.4%) were male. Mean age was 39.2±12.5. Three hundred and two (96.1%) were having social insurance coverage. Numbers of the known hepatitis B carrier as HbsAg positive were 16 (4.2%) and immunized (Anti-HBs positive) ones were 50 (13.3%). Twelve cases (3.2%) had the history of hepatitis B vaccination. All the cases who had vaccination wer...

Türk Mikrobiyoloji Cemiyeti Dergisi

Tez (Yüksek Lisans) -- İstanbul Teknik Üniversitesi, Fen Bilimleri Enstitüsü, 2006Thesis (M.Sc.) ... more Tez (Yüksek Lisans) -- İstanbul Teknik Üniversitesi, Fen Bilimleri Enstitüsü, 2006Thesis (M.Sc.) -- İstanbul Technical University, Institute of Science and Technology, 2006Bu çalışmada, çelik liflerin mekanik özellikleri ve kompozitlerin özelliklerini incelemek için kancalı ve/veya kancasız 3 farklı çelik tel kullanıldı. Her çelik lif hacim oranı değişkendi fakat toplam hacim % 2’de sabit tutuldu. Kısa kancasız düz çelik lifler,yüksek dayanımlı tunç kaplı olup, 6 mm. boyunda ve 0,16 mm. çapındadır. Normal dayanımlı çelik liflerin narinlikleri 55 ve 65 dir ve dayanımları aynıdır (1150 MPa). Düz kesimli çelik liflerin çekme dayanımları 2250 MPa dır. Farklı lifli toplam lifli ve lifsiz 12 beton karışımı üretildi. Karma lifli ve/veya lifsiz çelik tel içeren betonların basınç dayanımları, elastisite modülleri, net eğilme dayanımları, yarmada çekme dayanımları ve kırılma enerjileri yalın betonunkilerle karşılaştırıldı. Kısa liflerin,mikro çatlakların önlemesinde köprü görevi gördüğü ve bu...

Viral Hepatitis Journal

Objectives: Chronic hepatitis C virus (HCV) infection still continues to be a significant health ... more Objectives: Chronic hepatitis C virus (HCV) infection still continues to be a significant health problem in the entire world. In addition to this, knowing about the existing genotypes in a region is highly important in terms of guiding antiviral treatment and understanding the epidemiology in that region. In our study, we examined the varying genotype distribution in our region and affecting factors. Materials and Methods: To determine the HCV genotype distribution in Kahramanmaraş in Turkey, patients who were determined to be HCV-positive in the last 4.5 years and whose genotypes were studied retrospectively searched from records and included in the study. Results: Genotype 1 was the most prevalent genotype (47%) in Kahramanmaraş. The second most prevalent (45%) genotype was genotype 3. Additionally, genotypes 2 and 4 were seen at the rates of respectively 2% and 6%. While there was male dominance in genotypes 1, 2, and 3, genotype 4 had female dominance (69%). There was a very high male dominance in genotype 3 (95%), and the mean age of the patients was 26.4. Conclusion: The epidemiology of HCV may show serious variations at locations that receive intense migration and where increased drug usage is observed. The main point in preventing HCV infection should be the identification and elimination of risk factors.

KSÜ Tıp Fakültesi dergisi, 2005

Türk mikrobiyoloji cemiyeti dergisi, 2017

Eastern Journal Of Medicine, 2019

In the patients who have perioperative renal failure risk, anesthetical substances should be choo... more In the patients who have perioperative renal failure risk, anesthetical substances should be choosen with caution to protect the function of kidneys. Ketamine, an anesthetic induction agent, is generally used in patients with severe hypotension or respiratory depression. We aimed to evaluate the different doses of ketamine's effects on ischemia/reperfusion (I/R) damage mediated by free radicals in rats. Materials and methods: In this study, 42 Wistar albino male rats were splitted randomly into 7 different groups. In the ketamine group, ketamine was applied intraperitoneally (IP) in different doses (3 mg kg‫-‬ 1 , 10 mg kg‫-‬ 1 , 30 mg kg‫-‬ 1 , 60 mg kg‫-‬ 1 , 80 mg kg‫-‬ 1) on the 45 th minutes. Clamps were opened at the end of 60 minutes ischemia period. At the end of the reperfusion period, renal tissue and blood sample s were taken from the rats. In the plasma samples, pro-inflammatory biomarkers [Interleukin (IL)-1β, IL-6, tumour necrosis factor alpha (TNF-α)] were analysed. In renal tissue samples, antioxidating activities [Superoxide dismutase (SOD), glutathione perox idase (GPx) and nitric oxide (NO)] and lipid peroxidation product "Malondialdehyde (MDA)" levels were studied biochemically. Renal tissue damage was evaluated histopathologically. There were no differences among the beneficial effects of ketamine given gro ups (10-30-60-80 mg kg‫-‬ 1 doses) before reperfusion in the way of antioxidant activities, pro-inflammatory markers and lipid peroxidation product. When ketamin was applied in 3 mg kg‫-‬ 1 there were beneficial effects on tissues in the way of SOD, GPx, NO, MDA values and histopathologically (p<0.05). Some studies have shown that ketamine has little anti-inflammatory properties. This animal study has shown that ketamine in low doses significantly reduces the I/R injury in rats (p<0.05).