Mustafa Zeyadi - Academia.edu (original) (raw)

Papers by Mustafa Zeyadi

Research paper thumbnail of Effect of organic additives on storage stability of camel liver catalase against environmental conditions

Main Group Chemistry

The storage stability of catalase is very low under practical reaction conditions. Therefore, thi... more The storage stability of catalase is very low under practical reaction conditions. Therefore, this study is aimed to evaluate the storage stability of catalase from camel liver by treated it with polyethylene glycol (PEG), glycerol, bovine serum albumin (BSA) and glucose against different storage conditions. The effect of some additives as stabilizers on the storage stability of camel liver catalase at 4°C and 30°C after 30 and 90 days was studied. The enzyme with additives at 5%concentration was more stable at 4 and 30°C for 90 days of incubation than native enzyme. The activity of catalase was more thermal stable in presence of 5%additive. The activity of enzyme with 5%BSA and glucose was retained 60%of its activity at 60°C. At pH 11 the catalase with glucose retained 30%of its activity. The native enzyme lost 80%of its activity in presence of 5 mM β-mercaptoethanol, where the enzyme with additive retained 30–46%of its activity. At 8 M urea, the native enzyme and enzyme with 5%of ...

Research paper thumbnail of Pathobiological Relationship of Excessive Dietary Intake of Choline/L-Carnitine: A TMAO Precursor-Associated Aggravation in Heart Failure in Sarcopenic Patients

Nutrients

The microecological environment of the gastrointestinal tract is altered if there is an imbalance... more The microecological environment of the gastrointestinal tract is altered if there is an imbalance between the gut microbiota phylases, resulting in a variety of diseases. Moreover, progressive age not only slows down physical activity but also reduces the fat metabolism pathway, which may lead to a reduction in the variety of bacterial strains and bacteroidetes’ abundance, promoting firmicutes and proteobacteria growth. As a result, dysbiosis reduces physiological adaptability, boosts inflammatory markers, generates ROS, and induces the destruction of free radical macromolecules, leading to sarcopenia in older patients. Research conducted at various levels indicates that the microbiota of the gut is involved in pathogenesis and can be considered as the causative agent of several cardiovascular diseases. Local and systematic inflammatory reactions are caused in patients with heart failure, as ischemia and edema are caused by splanchnic hypoperfusion and enable both bacterial metaboli...

Research paper thumbnail of Comparative Study of the Efficacy of Prebiotics and Probiotics as Dietary Supplements in Rats with Gastric Ulcer

Journal of Pharmaceutical Research International

Gastric ulcer is considered as main problem of gastrointestinal disease, presenting one of the mo... more Gastric ulcer is considered as main problem of gastrointestinal disease, presenting one of the most important health issues in all societies. This comparative study designed to explore the anti-ulcerogenic role of probiotics and prebiotics and to evaluate the most potent effect against gastric ulcer. Sixty rats were distributed into 6 groups : G1 Healthy control; G2 gastric ulcer (GU), ulcer was induced by four oral doses of Aspirin 200 mg/kg b.w./week ;G3 Prebiotics (PreB) administered orally group ( 0.5 g/day); G4 Probiotics (ProB) administered orally group ( 0.5 mg/day); G5 Prebiotics (Therapeutic): prebiotics was administered orally at dose ( 0.5g/day) for 30 days after induction of gastric ulcer (GU+PreB); G6 Probiotics (Therapeutic): probiotics was administered orally at dose ( 0.5mg/day) for 30 days after induction of gastric ulcer (GU+ProB). Oxidative stress parameters and inflammatory markers were measured. Results revealed that: aspirin caused gastric ulcer associated with...

Research paper thumbnail of Recombinantl-glutaminase obtained fromGeobacillus thermodenitrificansDSM-465: characterization andin silicoelucidation of conserved structural domains

RSC Advances

Glutaminase (GLS) is an enzyme essential for amino acid metabolism; in particular, it acts as a c... more Glutaminase (GLS) is an enzyme essential for amino acid metabolism; in particular, it acts as a catalyst in glutaminolysis, a reaction exploited by the malignant cells to meet the nutrient requirements for their accelerated growth and proliferation.

Research paper thumbnail of Polio Elimination in North-West Pakistan Faces Setbacks in War-Affected Areas

Asia Pacific Journal of Public Health

Research paper thumbnail of A novel peroxidase from Ziziphus jujuba fruit: purification, thermodynamics and biochemical characterization properties

Scientific Reports

stage, a corresponding electron donor reduces Cpd I to Cpd II and to a free radical (2). Then the... more stage, a corresponding electron donor reduces Cpd I to Cpd II and to a free radical (2). Then the second substrate further lowers Cpd II to restore the enzyme's resting state and a radical one (3) 13 .

Research paper thumbnail of Impact of Caffeic Acid Phenylester on Nitric Oxide Synthase and Arginase in Rats Intoxicated with Nitrites

Journal of King Saud University - Science

Research paper thumbnail of Potential Antioxidant and Anti-Proliferative Activities of Biologically Active Marine Algae Extracts

Journal of Pharmaceutical Research International

Background: Marine microorganisms such as algae were identified a rich sources of biologically ac... more Background: Marine microorganisms such as algae were identified a rich sources of biologically active compounds. This study was designed to explore the in vitro antioxidant, anti-apoptotic and anti proliferative activities of different algae [Gracilaria dendroides (GD), Dictyota ciliolata (DC) and Cladophora socialis (CS)] extracts obtained from Red sea at Jeddah. Materials and Methods: Algae extracts (ethanol, chloroform and water) were tested for antioxidant activity for free radical scavenging and secondly as anti-apoptotic and anti-proliferative activity against different cell lines. Antioxidant capacity was detected by evaluation superoxide anion radicals, reducing potential and assay of phenolic and flavonoid content in extracts. Results: Chloroform extract of DC (CEDC) was found to have a better free radical scavenging activity (IC50= 310.58 g/ml) than methanol extract (MEDC). MEDC (IC50= 522 g/ml). The phenol level in CEDC and MEDC were 50.1 and 38.18 mg, while flavonoids were 65.58and 41.74 /mg.EDC and EDC were showed ant proliferative against MC7 and HALA 1. Conclusion: The solvents extracts exhibited promising biological activity as antioxidants and ant proliferative action.

Research paper thumbnail of Purification and characterization of peroxidase from date palm cv. Agwa fruits

International Journal of Food Properties

Purification of peroxidase POII from date palm cv. Agwa fruits were carried out. The molecular ma... more Purification of peroxidase POII from date palm cv. Agwa fruits were carried out. The molecular mass of POII was 43 kDa. The broad pH optimum of POII at pH's 6.0-7.0 was detected. The maximum activity of POII was detected at 60°C and the enzyme was thermally stable up to 60°C. POII oxidized phenolic compounds such as guaiacol, o-phenylenediamine, o-dianosidine, p-aminoantipyrine, pyrogallol and ABTS in presence of H 2 O 2 as second substrate. The Km values of POII were found to be 6 mM for H 2 O 2 and 25 mM for guaiacol. The enzyme activity of POII was enhanced by Fe +3 , Cu +2 , and Co +2. In conclusion, POII oxidized some of phenolic compounds, which caused the browning color in tamer stage of date cv. Agwa.

Research paper thumbnail of Zika Virus Targeting by Screening Inhibitors against NS2B/NS3 Protease

BioMed Research International

Zika flavivirus is suspected to cause Guillain-Barre syndrome in adults and microcephaly, along w... more Zika flavivirus is suspected to cause Guillain-Barre syndrome in adults and microcephaly, along with other congenital abnormalities in infants. Presently, no vaccines or therapeutics are available. Here, we report novel compounds identified by high-throughput virtual screening of Maybridge chemical database and molecular docking studies. We selected viral enzyme NS2B/NS3 serine protease as the therapeutic target because of its important role in viral replication. We selected seven potential compounds as antiviral drug candidates because of their high GOLD fitness score, high AutoDock Vina score, or X-Score binding energy and analyzed the strength of molecular interactions between the active site amino acids and selected compounds. Our study also provides a foundation for similar studies for the search of novel therapeutics against Zika virus.

Research paper thumbnail of In vitro Antiglycation, Antioxidant and Antiproliferative Properties of Peptides Derived from Tryptic Hydrolysis of Soya Bean

Journal of Pharmaceutical Research International

Aims: Bioactive peptides are acknowledged for their vital contributions to health promotion. This... more Aims: Bioactive peptides are acknowledged for their vital contributions to health promotion. This study aims to evaluate antiglycation, antioxidant and antiproliferative ability of peptides derived from tryptic hydrolysis of Soya bean. Methodology: Hydrolysis profile was qualified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Reducing sugars-trapping ability of peptides was examined. 0.5{\%} methacrylamido phenylboronic acid (MPBA) gel was used to investigate the advanced glycation end products (AGEs) inhibition. The in vitro antioxidant activity of peptides were tested by 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay. MCF-7, PC3, and HepG2 cell lines were used to explore the antiproliferative potency of peptides. Results: Peptide fractions trapped reducing sugars and reduced AGEs formation, and exhibited well DPPH scavenging ability with IC 50 8.96 mg/mL. Peptide fractions showed dose-dependent toxicity to the used cell lines. Conclusion: Released bioactive peptides emphasized the value of Soya bean proteins to be used as a functional food or alternative supplementary to diseases.

Research paper thumbnail of Thymoquinone induces cell proliferation inhibition and apoptosis in acute myeloid leukemia cells: role of apoptosis-related WT1 and BCL2 genes

European Journal of Cell Science

Acute myeloid leukemia (AML) is an aggressive and heterogeneous disease characterized by an abnor... more Acute myeloid leukemia (AML) is an aggressive and heterogeneous disease characterized by an abnormal proliferation and impaired differentiation of the myeloid precursor cells. The outcome for most AML patients remains poor with high relapse rates and chemotherapy remains the first line treatment for AML. The Wilms tumor wt1 and the anti-apoptotic BCL2 genes are upregulated in AML and are known to be involved in apoptosis inhibition. In the present study we evaluated the molecular mechanisms underlie the anti-proliferative and pro-apoptotic activities exerted by thymoquinone (TQ), the major biologically active compound of the black seed oil on acute myeloid leukemia (AML) cell line-HL60. Cell proliferation was determined by WST-1 assay and apoptosis rate was assessed by flow cytometry using annexin-V/7AAD staining. The expression of target genes was analyzed by real-time RT–PCR analysis. TQ significantly reduced HL60 cell viability and induced apoptosis in a dose and time-dependent m...

Research paper thumbnail of The human papillomavirus, cervical cancer and screening strategies: an update

Biomedical Research

Cervical cancer, the most common gynaecological malignancy has been strongly correlated with the ... more Cervical cancer, the most common gynaecological malignancy has been strongly correlated with the infection of human papillomavirus (HPV). Different risk factors exaggerate the onset and progression of cervical cancer to malignant and metastatic state. Recent outcomes for the identification of the molecular pathways involved in cervical cancer provide clues about novel bio or oncogenic markers that are proved to be helpful in monitoring the detection of lesion with a high risk of progression in cytological smears and histological specimens. Sexual health programs and public awareness with developing strategies is the ultimate requirement for combating cervical cancer worldwide. The current review discusses mechanism of HPV that links with cervical cancer, various risk factors for the progression of cervical pathologies including smoking, oral contraceptives and sexual factors as well as diagnostic and screening strategies for cervical cancer.

Research paper thumbnail of Middle East respiratory syndrome: pathogenesis and therapeutic developments

Future Virology

The first case of Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in the y... more The first case of Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in the year 2012, which spread rapidly and increased to more than 2200 in 2018. This highly pathogenic virus with high mortality rate is among one of the major public health concerns. Saudi Arabia remains to be the most affected region with the majority of MERS-CoV cases, and currently, no effective drugs and vaccines are available for prevention and treatment. A large amount of information is now available regarding the virus, its structure, route of transmission and its pathophysiology. Therefore, this review summarizes the current understanding of MERS-CoV's pathogenesis, treatment options and recent scientific advancements in vaccine and other therapeutic developments, and the major steps taken for MERS prevention control.

Research paper thumbnail of Synthesis and in vitro antitumor activity of novel acylspermidine derivative N-(4-aminobutyl)-N-(3-aminopropyl)-8-hydroxy-dodecanamide (AAHD) against HepG2 cells

Research paper thumbnail of Antiglycation and Fatty Acids Profiling in Response to Phyocyanin Extracted from Chlorophyta Ulva lactuca Algae Loaded on Albumin Nano-particles (ULANP) in Diabetic Rats

Journal of Pharmaceutical Research International

Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae ... more Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae loaded on albumin nano-particles (ULANP) on diabetic rats. Materials and Methods: Fifty albino rats were divided into 5 goups. GPI: control and GPII: rats were injected with alloxan (75 mg /kg) i.p for six consecutive days for induction of diabetes. This group was subdivided into 4 subgroups: GP IIa: (Untreated diabetic): GP IIb: rats were given with ULANP (100 mg/kg).GP IIc: Rats were given ULANP (200 mg/kg) i.p. GP IId: Rats were given insulin (100 unit/ day). Serum NO, interleukin-6 glucose, AGEs and fatty acids profile was determined. Results: Analysis of ULANP by FTIR showed the characteristic band (2100 cm-1~ 3700 cm-1) that is indicated mainly from -COO, – CO and conjugated double bond. These bonds showed spectral bands peak 2985 cm-1 and 2860 cm-1, 2986 cm-1. Administration of ULANP in diabetic rats exerted an anti-inflammatory by lowering NO and IL-6 levels and hypoglycemic e...

Research paper thumbnail of In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

Journal of Pharmaceutical Research International

Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that ... more Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that lead to oxidative damage of biologically active molecules, finally lead to different disease and initiate carcinogenesis. The drug discovery using natural products as medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extract obtained from Red sea at Jeddah. Materials and Methods: Aqueousand methanol extracts ofDictyotaciliolata(DC) were tested on HCT-116 and HepG2 cell lines using WST-1. Aqueous extract (AEDC) and methanol extract (MEDC) at doses 0.05, 0.1, 0.5, 1 mg/ml and positive control 0.3% H2O2 at doses 0.5 mg/ml for 24,48 and 72 h (for two cell lines). Results: AEDC showed the mosteffective antitumor activity against HCT116 and HePG2, the IC50 dose for HCT116 cells was 0.05 mg/ml at 72 h, while for the HePG2 it was 0.01 mg/ml at 72 h. These results showed that HePG2cells was more sensitive to the...

Research paper thumbnail of Modulation the Neuro-toxicity Induced by Aluminum Chloride in Rats Using Beetroots and Broccli Extracts

Journal of Pharmaceutical Research International

Backgound: The generation of oxidative stress can be referred to Aluminium toxic effect in animal... more Backgound: The generation of oxidative stress can be referred to Aluminium toxic effect in animals and humans. This study aimed to evaluate the role of broccoli (Br) and beetroot (Be) extarcts as antioxidant that prevents oxidative stress that associated with aluminum toxicity. Materials and Methods: Fifty Wister female rats were grouped into five groups (each 10 rats): Group 1: control group, administered drinking water only. Group 2: (Neurogenerative) which were induced by oral administration of aluminum chloride (20 mg/kg b.w) daily for one month. Group 3: Rats given aluminum chloride were treated with Rivastigmine (Ri) (1 mg/kg b.w) as a reference drug daily for five weeks. Group 4: Rats given aluminum chloride were treated with beet root extract (50 mg/kg b.w) daily for six weeks. Group 5. Rats given aluminum chloride were treated with broccoli extract (50 mg/kg b.w) daily for five weeks. Results: (AlCl3) group showed a significant increase in Ach level (P<0.05) and a non-si...

Research paper thumbnail of Lipidomic Profiling Disturbance as Good Follow up Index in Obese Diabetic Patients

Journal of Pharmaceutical Research International

Research paper thumbnail of Inter-relationship of circulating biochemical markers of oxidative stress and comorbid condition in polycystic ovary syndrome

Biomedical Research

Introduction: Polycystic Ovarian Syndrome (PCOS), commonly found in 5% of all women of reproducti... more Introduction: Polycystic Ovarian Syndrome (PCOS), commonly found in 5% of all women of reproductive age is a heterogeneous endocrine disorder in which woman's levels of estrogen and progesterone are imbalance leading to the growth of benign masses in the ovaries (ovarian cysts). Rotterdam criteria used to diagnose PCOS include two of three clinical features: 1) oligo-or anovulation, 2) hyperandroginism e.g., hirsutism, acne etc. and 3) sonographic morphologic evidence of polycystic ovaries. Aim: To investigate the relationship between circulating biomarkers and their heterogeneity in PCOS. Material and methods: A prospective case control study was conducted including 70 females of age ranging from 20 to 35 y out of which 20 served as healthy controls and 50 were patients of PCOS. Lipid levels, renal profile and antioxidative status of Malondialdehyde (MDA), Catalase (CAT), Superoxide dismutase (SOD) and Reduced glutathione (GSH) was analysed quantitatively. Serum electrolytes (Na + , K + , Mg 2+ , PO 4-3), anti-oxidants vitamins C and E and steroidal hormones like Follicle Stimulating Hormone (FSH), Sex Hormone Binding Globulin (SHBG) were measured by the commercially available kits. Results: A total of 70 patients were enrolled to the study. Serum electrolytes level like potassium (K) and Phosphorus (P) were significantly increased while antioxidants were decreased in the study group as compared to the control group. Oxidative stress was also increased with higher levels of MDA. Follicle Stimulating Hormone (FSH) and Sex Hormone Binding Globulin (SHBG) were decreased in the study group. The PCOS patients showed statistically significant correlation among the circulating biomarkers like FSH vs. prolactin (r=0.400 **).

Research paper thumbnail of Effect of organic additives on storage stability of camel liver catalase against environmental conditions

Main Group Chemistry

The storage stability of catalase is very low under practical reaction conditions. Therefore, thi... more The storage stability of catalase is very low under practical reaction conditions. Therefore, this study is aimed to evaluate the storage stability of catalase from camel liver by treated it with polyethylene glycol (PEG), glycerol, bovine serum albumin (BSA) and glucose against different storage conditions. The effect of some additives as stabilizers on the storage stability of camel liver catalase at 4°C and 30°C after 30 and 90 days was studied. The enzyme with additives at 5%concentration was more stable at 4 and 30°C for 90 days of incubation than native enzyme. The activity of catalase was more thermal stable in presence of 5%additive. The activity of enzyme with 5%BSA and glucose was retained 60%of its activity at 60°C. At pH 11 the catalase with glucose retained 30%of its activity. The native enzyme lost 80%of its activity in presence of 5 mM β-mercaptoethanol, where the enzyme with additive retained 30–46%of its activity. At 8 M urea, the native enzyme and enzyme with 5%of ...

Research paper thumbnail of Pathobiological Relationship of Excessive Dietary Intake of Choline/L-Carnitine: A TMAO Precursor-Associated Aggravation in Heart Failure in Sarcopenic Patients

Nutrients

The microecological environment of the gastrointestinal tract is altered if there is an imbalance... more The microecological environment of the gastrointestinal tract is altered if there is an imbalance between the gut microbiota phylases, resulting in a variety of diseases. Moreover, progressive age not only slows down physical activity but also reduces the fat metabolism pathway, which may lead to a reduction in the variety of bacterial strains and bacteroidetes’ abundance, promoting firmicutes and proteobacteria growth. As a result, dysbiosis reduces physiological adaptability, boosts inflammatory markers, generates ROS, and induces the destruction of free radical macromolecules, leading to sarcopenia in older patients. Research conducted at various levels indicates that the microbiota of the gut is involved in pathogenesis and can be considered as the causative agent of several cardiovascular diseases. Local and systematic inflammatory reactions are caused in patients with heart failure, as ischemia and edema are caused by splanchnic hypoperfusion and enable both bacterial metaboli...

Research paper thumbnail of Comparative Study of the Efficacy of Prebiotics and Probiotics as Dietary Supplements in Rats with Gastric Ulcer

Journal of Pharmaceutical Research International

Gastric ulcer is considered as main problem of gastrointestinal disease, presenting one of the mo... more Gastric ulcer is considered as main problem of gastrointestinal disease, presenting one of the most important health issues in all societies. This comparative study designed to explore the anti-ulcerogenic role of probiotics and prebiotics and to evaluate the most potent effect against gastric ulcer. Sixty rats were distributed into 6 groups : G1 Healthy control; G2 gastric ulcer (GU), ulcer was induced by four oral doses of Aspirin 200 mg/kg b.w./week ;G3 Prebiotics (PreB) administered orally group ( 0.5 g/day); G4 Probiotics (ProB) administered orally group ( 0.5 mg/day); G5 Prebiotics (Therapeutic): prebiotics was administered orally at dose ( 0.5g/day) for 30 days after induction of gastric ulcer (GU+PreB); G6 Probiotics (Therapeutic): probiotics was administered orally at dose ( 0.5mg/day) for 30 days after induction of gastric ulcer (GU+ProB). Oxidative stress parameters and inflammatory markers were measured. Results revealed that: aspirin caused gastric ulcer associated with...

Research paper thumbnail of Recombinantl-glutaminase obtained fromGeobacillus thermodenitrificansDSM-465: characterization andin silicoelucidation of conserved structural domains

RSC Advances

Glutaminase (GLS) is an enzyme essential for amino acid metabolism; in particular, it acts as a c... more Glutaminase (GLS) is an enzyme essential for amino acid metabolism; in particular, it acts as a catalyst in glutaminolysis, a reaction exploited by the malignant cells to meet the nutrient requirements for their accelerated growth and proliferation.

Research paper thumbnail of Polio Elimination in North-West Pakistan Faces Setbacks in War-Affected Areas

Asia Pacific Journal of Public Health

Research paper thumbnail of A novel peroxidase from Ziziphus jujuba fruit: purification, thermodynamics and biochemical characterization properties

Scientific Reports

stage, a corresponding electron donor reduces Cpd I to Cpd II and to a free radical (2). Then the... more stage, a corresponding electron donor reduces Cpd I to Cpd II and to a free radical (2). Then the second substrate further lowers Cpd II to restore the enzyme's resting state and a radical one (3) 13 .

Research paper thumbnail of Impact of Caffeic Acid Phenylester on Nitric Oxide Synthase and Arginase in Rats Intoxicated with Nitrites

Journal of King Saud University - Science

Research paper thumbnail of Potential Antioxidant and Anti-Proliferative Activities of Biologically Active Marine Algae Extracts

Journal of Pharmaceutical Research International

Background: Marine microorganisms such as algae were identified a rich sources of biologically ac... more Background: Marine microorganisms such as algae were identified a rich sources of biologically active compounds. This study was designed to explore the in vitro antioxidant, anti-apoptotic and anti proliferative activities of different algae [Gracilaria dendroides (GD), Dictyota ciliolata (DC) and Cladophora socialis (CS)] extracts obtained from Red sea at Jeddah. Materials and Methods: Algae extracts (ethanol, chloroform and water) were tested for antioxidant activity for free radical scavenging and secondly as anti-apoptotic and anti-proliferative activity against different cell lines. Antioxidant capacity was detected by evaluation superoxide anion radicals, reducing potential and assay of phenolic and flavonoid content in extracts. Results: Chloroform extract of DC (CEDC) was found to have a better free radical scavenging activity (IC50= 310.58 g/ml) than methanol extract (MEDC). MEDC (IC50= 522 g/ml). The phenol level in CEDC and MEDC were 50.1 and 38.18 mg, while flavonoids were 65.58and 41.74 /mg.EDC and EDC were showed ant proliferative against MC7 and HALA 1. Conclusion: The solvents extracts exhibited promising biological activity as antioxidants and ant proliferative action.

Research paper thumbnail of Purification and characterization of peroxidase from date palm cv. Agwa fruits

International Journal of Food Properties

Purification of peroxidase POII from date palm cv. Agwa fruits were carried out. The molecular ma... more Purification of peroxidase POII from date palm cv. Agwa fruits were carried out. The molecular mass of POII was 43 kDa. The broad pH optimum of POII at pH's 6.0-7.0 was detected. The maximum activity of POII was detected at 60°C and the enzyme was thermally stable up to 60°C. POII oxidized phenolic compounds such as guaiacol, o-phenylenediamine, o-dianosidine, p-aminoantipyrine, pyrogallol and ABTS in presence of H 2 O 2 as second substrate. The Km values of POII were found to be 6 mM for H 2 O 2 and 25 mM for guaiacol. The enzyme activity of POII was enhanced by Fe +3 , Cu +2 , and Co +2. In conclusion, POII oxidized some of phenolic compounds, which caused the browning color in tamer stage of date cv. Agwa.

Research paper thumbnail of Zika Virus Targeting by Screening Inhibitors against NS2B/NS3 Protease

BioMed Research International

Zika flavivirus is suspected to cause Guillain-Barre syndrome in adults and microcephaly, along w... more Zika flavivirus is suspected to cause Guillain-Barre syndrome in adults and microcephaly, along with other congenital abnormalities in infants. Presently, no vaccines or therapeutics are available. Here, we report novel compounds identified by high-throughput virtual screening of Maybridge chemical database and molecular docking studies. We selected viral enzyme NS2B/NS3 serine protease as the therapeutic target because of its important role in viral replication. We selected seven potential compounds as antiviral drug candidates because of their high GOLD fitness score, high AutoDock Vina score, or X-Score binding energy and analyzed the strength of molecular interactions between the active site amino acids and selected compounds. Our study also provides a foundation for similar studies for the search of novel therapeutics against Zika virus.

Research paper thumbnail of In vitro Antiglycation, Antioxidant and Antiproliferative Properties of Peptides Derived from Tryptic Hydrolysis of Soya Bean

Journal of Pharmaceutical Research International

Aims: Bioactive peptides are acknowledged for their vital contributions to health promotion. This... more Aims: Bioactive peptides are acknowledged for their vital contributions to health promotion. This study aims to evaluate antiglycation, antioxidant and antiproliferative ability of peptides derived from tryptic hydrolysis of Soya bean. Methodology: Hydrolysis profile was qualified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Reducing sugars-trapping ability of peptides was examined. 0.5{\%} methacrylamido phenylboronic acid (MPBA) gel was used to investigate the advanced glycation end products (AGEs) inhibition. The in vitro antioxidant activity of peptides were tested by 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay. MCF-7, PC3, and HepG2 cell lines were used to explore the antiproliferative potency of peptides. Results: Peptide fractions trapped reducing sugars and reduced AGEs formation, and exhibited well DPPH scavenging ability with IC 50 8.96 mg/mL. Peptide fractions showed dose-dependent toxicity to the used cell lines. Conclusion: Released bioactive peptides emphasized the value of Soya bean proteins to be used as a functional food or alternative supplementary to diseases.

Research paper thumbnail of Thymoquinone induces cell proliferation inhibition and apoptosis in acute myeloid leukemia cells: role of apoptosis-related WT1 and BCL2 genes

European Journal of Cell Science

Acute myeloid leukemia (AML) is an aggressive and heterogeneous disease characterized by an abnor... more Acute myeloid leukemia (AML) is an aggressive and heterogeneous disease characterized by an abnormal proliferation and impaired differentiation of the myeloid precursor cells. The outcome for most AML patients remains poor with high relapse rates and chemotherapy remains the first line treatment for AML. The Wilms tumor wt1 and the anti-apoptotic BCL2 genes are upregulated in AML and are known to be involved in apoptosis inhibition. In the present study we evaluated the molecular mechanisms underlie the anti-proliferative and pro-apoptotic activities exerted by thymoquinone (TQ), the major biologically active compound of the black seed oil on acute myeloid leukemia (AML) cell line-HL60. Cell proliferation was determined by WST-1 assay and apoptosis rate was assessed by flow cytometry using annexin-V/7AAD staining. The expression of target genes was analyzed by real-time RT–PCR analysis. TQ significantly reduced HL60 cell viability and induced apoptosis in a dose and time-dependent m...

Research paper thumbnail of The human papillomavirus, cervical cancer and screening strategies: an update

Biomedical Research

Cervical cancer, the most common gynaecological malignancy has been strongly correlated with the ... more Cervical cancer, the most common gynaecological malignancy has been strongly correlated with the infection of human papillomavirus (HPV). Different risk factors exaggerate the onset and progression of cervical cancer to malignant and metastatic state. Recent outcomes for the identification of the molecular pathways involved in cervical cancer provide clues about novel bio or oncogenic markers that are proved to be helpful in monitoring the detection of lesion with a high risk of progression in cytological smears and histological specimens. Sexual health programs and public awareness with developing strategies is the ultimate requirement for combating cervical cancer worldwide. The current review discusses mechanism of HPV that links with cervical cancer, various risk factors for the progression of cervical pathologies including smoking, oral contraceptives and sexual factors as well as diagnostic and screening strategies for cervical cancer.

Research paper thumbnail of Middle East respiratory syndrome: pathogenesis and therapeutic developments

Future Virology

The first case of Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in the y... more The first case of Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in the year 2012, which spread rapidly and increased to more than 2200 in 2018. This highly pathogenic virus with high mortality rate is among one of the major public health concerns. Saudi Arabia remains to be the most affected region with the majority of MERS-CoV cases, and currently, no effective drugs and vaccines are available for prevention and treatment. A large amount of information is now available regarding the virus, its structure, route of transmission and its pathophysiology. Therefore, this review summarizes the current understanding of MERS-CoV's pathogenesis, treatment options and recent scientific advancements in vaccine and other therapeutic developments, and the major steps taken for MERS prevention control.

Research paper thumbnail of Synthesis and in vitro antitumor activity of novel acylspermidine derivative N-(4-aminobutyl)-N-(3-aminopropyl)-8-hydroxy-dodecanamide (AAHD) against HepG2 cells

Research paper thumbnail of Antiglycation and Fatty Acids Profiling in Response to Phyocyanin Extracted from Chlorophyta Ulva lactuca Algae Loaded on Albumin Nano-particles (ULANP) in Diabetic Rats

Journal of Pharmaceutical Research International

Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae ... more Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae loaded on albumin nano-particles (ULANP) on diabetic rats. Materials and Methods: Fifty albino rats were divided into 5 goups. GPI: control and GPII: rats were injected with alloxan (75 mg /kg) i.p for six consecutive days for induction of diabetes. This group was subdivided into 4 subgroups: GP IIa: (Untreated diabetic): GP IIb: rats were given with ULANP (100 mg/kg).GP IIc: Rats were given ULANP (200 mg/kg) i.p. GP IId: Rats were given insulin (100 unit/ day). Serum NO, interleukin-6 glucose, AGEs and fatty acids profile was determined. Results: Analysis of ULANP by FTIR showed the characteristic band (2100 cm-1~ 3700 cm-1) that is indicated mainly from -COO, – CO and conjugated double bond. These bonds showed spectral bands peak 2985 cm-1 and 2860 cm-1, 2986 cm-1. Administration of ULANP in diabetic rats exerted an anti-inflammatory by lowering NO and IL-6 levels and hypoglycemic e...

Research paper thumbnail of In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

Journal of Pharmaceutical Research International

Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that ... more Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that lead to oxidative damage of biologically active molecules, finally lead to different disease and initiate carcinogenesis. The drug discovery using natural products as medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extract obtained from Red sea at Jeddah. Materials and Methods: Aqueousand methanol extracts ofDictyotaciliolata(DC) were tested on HCT-116 and HepG2 cell lines using WST-1. Aqueous extract (AEDC) and methanol extract (MEDC) at doses 0.05, 0.1, 0.5, 1 mg/ml and positive control 0.3% H2O2 at doses 0.5 mg/ml for 24,48 and 72 h (for two cell lines). Results: AEDC showed the mosteffective antitumor activity against HCT116 and HePG2, the IC50 dose for HCT116 cells was 0.05 mg/ml at 72 h, while for the HePG2 it was 0.01 mg/ml at 72 h. These results showed that HePG2cells was more sensitive to the...

Research paper thumbnail of Modulation the Neuro-toxicity Induced by Aluminum Chloride in Rats Using Beetroots and Broccli Extracts

Journal of Pharmaceutical Research International

Backgound: The generation of oxidative stress can be referred to Aluminium toxic effect in animal... more Backgound: The generation of oxidative stress can be referred to Aluminium toxic effect in animals and humans. This study aimed to evaluate the role of broccoli (Br) and beetroot (Be) extarcts as antioxidant that prevents oxidative stress that associated with aluminum toxicity. Materials and Methods: Fifty Wister female rats were grouped into five groups (each 10 rats): Group 1: control group, administered drinking water only. Group 2: (Neurogenerative) which were induced by oral administration of aluminum chloride (20 mg/kg b.w) daily for one month. Group 3: Rats given aluminum chloride were treated with Rivastigmine (Ri) (1 mg/kg b.w) as a reference drug daily for five weeks. Group 4: Rats given aluminum chloride were treated with beet root extract (50 mg/kg b.w) daily for six weeks. Group 5. Rats given aluminum chloride were treated with broccoli extract (50 mg/kg b.w) daily for five weeks. Results: (AlCl3) group showed a significant increase in Ach level (P<0.05) and a non-si...

Research paper thumbnail of Lipidomic Profiling Disturbance as Good Follow up Index in Obese Diabetic Patients

Journal of Pharmaceutical Research International

Research paper thumbnail of Inter-relationship of circulating biochemical markers of oxidative stress and comorbid condition in polycystic ovary syndrome

Biomedical Research

Introduction: Polycystic Ovarian Syndrome (PCOS), commonly found in 5% of all women of reproducti... more Introduction: Polycystic Ovarian Syndrome (PCOS), commonly found in 5% of all women of reproductive age is a heterogeneous endocrine disorder in which woman's levels of estrogen and progesterone are imbalance leading to the growth of benign masses in the ovaries (ovarian cysts). Rotterdam criteria used to diagnose PCOS include two of three clinical features: 1) oligo-or anovulation, 2) hyperandroginism e.g., hirsutism, acne etc. and 3) sonographic morphologic evidence of polycystic ovaries. Aim: To investigate the relationship between circulating biomarkers and their heterogeneity in PCOS. Material and methods: A prospective case control study was conducted including 70 females of age ranging from 20 to 35 y out of which 20 served as healthy controls and 50 were patients of PCOS. Lipid levels, renal profile and antioxidative status of Malondialdehyde (MDA), Catalase (CAT), Superoxide dismutase (SOD) and Reduced glutathione (GSH) was analysed quantitatively. Serum electrolytes (Na + , K + , Mg 2+ , PO 4-3), anti-oxidants vitamins C and E and steroidal hormones like Follicle Stimulating Hormone (FSH), Sex Hormone Binding Globulin (SHBG) were measured by the commercially available kits. Results: A total of 70 patients were enrolled to the study. Serum electrolytes level like potassium (K) and Phosphorus (P) were significantly increased while antioxidants were decreased in the study group as compared to the control group. Oxidative stress was also increased with higher levels of MDA. Follicle Stimulating Hormone (FSH) and Sex Hormone Binding Globulin (SHBG) were decreased in the study group. The PCOS patients showed statistically significant correlation among the circulating biomarkers like FSH vs. prolactin (r=0.400 **).