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Papers by Mutia Hariani Nurjanah
Jurnal Biosains Pascasarjana, Jan 2, 2023
Salmonelosis adalah penyakit yang disebabkan bakteri Salmonella sp, ditularkan melalui makanan da... more Salmonelosis adalah penyakit yang disebabkan bakteri Salmonella sp, ditularkan melalui makanan dan minuman yang menyebabkan penurunan ekspresi CD4 +. Ekstrak buah anggur hitam memiliki kandungan polifenol, vitamin C, prosianidin, quercetin, resveratrol, antosianidin, flavonoid yang dapat meningkatkan sistem imunitas melalui proliferasi limfosit T CD4+/CD8+. Tujuan penelitian ini adalah untuk mengevaluasi aktivitas ekstrak anggur hitam terhadap ekspresi CD4 + dan CD8 + pada mencit balb/C terinfeksi Salmonella typhimurium. Metode penelitian ini adalah pembuatan ekstrak etanol 96% buah anggur hitam, infeksi bakteri Salmonella typhimurium 10 8 pada mencit, evaluasi bakterimia menggunakan pewarnaan giemsa, terapi ekstrak buah anggur hitam, pemeriksaan flowcytometeri, serta uji statistika menggunakan One Way Anova dengan uji lanjtuan tuckey 95%. Hasil analisa statistika One Way Anova menunjukkan bahwa ekspresi sel T CD4 + (0,72>0,05) dan sel T CD8 + (1,84>0,05) tidak terjadi perbedaan yang nyata. Namun secara biologis, terjadi peningkatan ekspresi CD4 + dan CD8 + pada dosis 100 mg/KgBB jika dibandingkan kelompok lain. Kemudian, terjadi penurunan ekspresi CD4 + dan CD8 + pada dosis 200 mg/KgBB dan 400 mg/KgBB jika dibandingkan dengan dosis 100 mg/KgBB. Kesimpulannya, secara biologis, ekstrak buah anggur hitam dapat meningkatkan ekspresi CD4 + dan CD8 + pada dosis rendah, serta dapat menurunkan ekspresi CD4 + dan CD8 + pada dosis sedang sampai tinggi.
Jurnal Biosains Pascasarjana, Jan 2, 2023
Infeksi Brucella Abortus pada sapi merupakan infeksi yang memiliki efek strategis pada masyarakat... more Infeksi Brucella Abortus pada sapi merupakan infeksi yang memiliki efek strategis pada masyarakat khususnya sector ekonomi. Penyakit ini disebabkan oleh bakteri gram negative yaitu Brucella Abortus. Penanganan Infeksi Brucella Abortus pada sapi masih belum efektif. Terapi berdasarkan respon imun yang ditimbulkan dari Infeksi Brucella Abortus terutama respon sitokin menjadi pilihan efektif mengingat setiap jenis sapi memiliki respon imun yang berbeda dalam menghadapi Infeksi Brucella Abortus. Tujuan penulisan literature review ini untuk mengidentifikasi sitokin-sitokin yang berperan dalam Infeksi Brucella Abortus pada sapi. Metode : pencarian otomatis pada data base PUBMED dengan menggunakan kata kunci "Brucella Abortus Bovine", "Cytokine", dan "Brucellosis". Hasil: 11 artikel yang relevan dan sesuai kriteria inklusi untuk menganalisa sitokin yang berperan dalam Infeksi Brucella Abortus pada sapi. Kesimpulan : Sitokin yang berperan dalam infeksi Brucella Abortus pada sapi umumnya adalah IL-1, IL-6, IL-12, IL-8, TNF-α, dan IFN-γ.
Jurnal Biosains Pascasarjana
Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression.... more Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression. Black grape can boost the immune system through CD4+/CD8+ proliferation. The purpose of this study was to evaluate the activity of black grape extract to CD4+ and CD8+ expression in mice infected with Salmonella typhimurium. Research method is extract of black grapes, Salmonella typhimurium 108 infection, bacterial evaluation, extract therapy, flowcytometery examination, and 95% Anova test. The results of Anova test showed that the expression of CD4+ and CD8+ is not different. Biologically, an increase in CD4+ and CD8+ expression at doses of 100 mg/KgBB. A decrease in CD4+ and CD8+ expression at doses of 200 mg/KgBB and 400 mg/KgBB. Conclusion, biologically, black grape extract can increase the expression of CD4+ and CD8+ at low doses, as well as may decrease the expression of CD4+ and CD8+ at moderate to high doses.
Jurnal Biosains Pascasarjana
Brucella Abortus infection in cattle is an infection that has a strategic effect on society, espe... more Brucella Abortus infection in cattle is an infection that has a strategic effect on society, especially the economic sector. This disease is caused by gram-negative bacteria, namely Brucella abortion. Treatment of Brucella Abortus Infection in cattle is still not effective. Therapy based on the immune response caused by Brucella Abortus Infection, especially the cytokine response, is an effective choice considering that each type of cow has a different immune response in dealing with Brucella Abortus Infection. The purpose of writing this literature review is to identify cytokines that play a role in Brucella Abortus Infection in cattle. Method: automated PUBMED database search using keywords “Brucella Abortus Bovine”, “Cytokine”, and “Brucellosis”. Results: 11 articles that are relevant and meet the inclusion criteria to analyze the cytokines that play a role in Brucella Abortus Infection in cattle. Conclusion: Cytokines that play a role in Brucella Abortus infection in cattle are ...
Jurnal Biosains Pascasarjana
Salmonella typhi is a gram-negative, intracellular facultative bacterium that can live and even r... more Salmonella typhi is a gram-negative, intracellular facultative bacterium that can live and even reproduce in macrophages, resistant to lysosomal enzymes, which has the ability to prevent phagosome-lysosome fusion, making it difficult to kill. stimulates the immune response in the host (Abbas et alll, 2016; Tores et alll, 2000). White turmeric (Curcuma zedoaria) is one of the herbal plants used as an immunomodulator. Immodulators in white turmeric substances function to increase the function of phagocytosis in macrophages to destroy Salmonella typhimurium bacteria. The purpose of this study was to determine the difference between white turmeric extract as an immunomodulator on the number of CD4+ T lymphocytes in BALB/c mice infected with Salmonella typhimurium. This research is a true experimental research with a posttest only control group design. Mice were divided into 5 groups consisting of negative control group, positive control and test group. Analysis of CD4+ lymphocyte data s...
Jurnal Biosains Pascasarjana
Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged ... more Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged hyperglycemia and elevated HbA1c levels as a standard test for diabetes mellitus. HbA1c describes the level of sugar in the blood for the last 3 months. One of the complications of diabetes mellitus is kidney damage. The state of kidney function can be described from the GFR (Glomerular Filtration Rate) examination as a standard examination for abnormalities in kidney disease. The purpose of this study was to determine the relationship between HbA1c levels and eGFR values in people with diabetes mellitus. The design of this study was a cross-sectional approach to 30 samples of DM patients who took Prolanis at the Ultra Medica Tulungagung Clinical Laboratory. The results showed that the average HbA1c level in all samples was 6.2% and the average eGFR value was 88.7 ml/min/1, 73m2. The Pearson correlation test obtained a ρ value = 0.000 so that it can be concluded that there is a relatio...
Medicra (Journal of Medical Laboratory Science/Technology), 2020
Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants... more Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 m...
Jurnal Biosains Pascasarjana, Jan 2, 2023
Salmonelosis adalah penyakit yang disebabkan bakteri Salmonella sp, ditularkan melalui makanan da... more Salmonelosis adalah penyakit yang disebabkan bakteri Salmonella sp, ditularkan melalui makanan dan minuman yang menyebabkan penurunan ekspresi CD4 +. Ekstrak buah anggur hitam memiliki kandungan polifenol, vitamin C, prosianidin, quercetin, resveratrol, antosianidin, flavonoid yang dapat meningkatkan sistem imunitas melalui proliferasi limfosit T CD4+/CD8+. Tujuan penelitian ini adalah untuk mengevaluasi aktivitas ekstrak anggur hitam terhadap ekspresi CD4 + dan CD8 + pada mencit balb/C terinfeksi Salmonella typhimurium. Metode penelitian ini adalah pembuatan ekstrak etanol 96% buah anggur hitam, infeksi bakteri Salmonella typhimurium 10 8 pada mencit, evaluasi bakterimia menggunakan pewarnaan giemsa, terapi ekstrak buah anggur hitam, pemeriksaan flowcytometeri, serta uji statistika menggunakan One Way Anova dengan uji lanjtuan tuckey 95%. Hasil analisa statistika One Way Anova menunjukkan bahwa ekspresi sel T CD4 + (0,72>0,05) dan sel T CD8 + (1,84>0,05) tidak terjadi perbedaan yang nyata. Namun secara biologis, terjadi peningkatan ekspresi CD4 + dan CD8 + pada dosis 100 mg/KgBB jika dibandingkan kelompok lain. Kemudian, terjadi penurunan ekspresi CD4 + dan CD8 + pada dosis 200 mg/KgBB dan 400 mg/KgBB jika dibandingkan dengan dosis 100 mg/KgBB. Kesimpulannya, secara biologis, ekstrak buah anggur hitam dapat meningkatkan ekspresi CD4 + dan CD8 + pada dosis rendah, serta dapat menurunkan ekspresi CD4 + dan CD8 + pada dosis sedang sampai tinggi.
Jurnal Biosains Pascasarjana, Jan 2, 2023
Infeksi Brucella Abortus pada sapi merupakan infeksi yang memiliki efek strategis pada masyarakat... more Infeksi Brucella Abortus pada sapi merupakan infeksi yang memiliki efek strategis pada masyarakat khususnya sector ekonomi. Penyakit ini disebabkan oleh bakteri gram negative yaitu Brucella Abortus. Penanganan Infeksi Brucella Abortus pada sapi masih belum efektif. Terapi berdasarkan respon imun yang ditimbulkan dari Infeksi Brucella Abortus terutama respon sitokin menjadi pilihan efektif mengingat setiap jenis sapi memiliki respon imun yang berbeda dalam menghadapi Infeksi Brucella Abortus. Tujuan penulisan literature review ini untuk mengidentifikasi sitokin-sitokin yang berperan dalam Infeksi Brucella Abortus pada sapi. Metode : pencarian otomatis pada data base PUBMED dengan menggunakan kata kunci "Brucella Abortus Bovine", "Cytokine", dan "Brucellosis". Hasil: 11 artikel yang relevan dan sesuai kriteria inklusi untuk menganalisa sitokin yang berperan dalam Infeksi Brucella Abortus pada sapi. Kesimpulan : Sitokin yang berperan dalam infeksi Brucella Abortus pada sapi umumnya adalah IL-1, IL-6, IL-12, IL-8, TNF-α, dan IFN-γ.
Jurnal Biosains Pascasarjana
Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression.... more Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression. Black grape can boost the immune system through CD4+/CD8+ proliferation. The purpose of this study was to evaluate the activity of black grape extract to CD4+ and CD8+ expression in mice infected with Salmonella typhimurium. Research method is extract of black grapes, Salmonella typhimurium 108 infection, bacterial evaluation, extract therapy, flowcytometery examination, and 95% Anova test. The results of Anova test showed that the expression of CD4+ and CD8+ is not different. Biologically, an increase in CD4+ and CD8+ expression at doses of 100 mg/KgBB. A decrease in CD4+ and CD8+ expression at doses of 200 mg/KgBB and 400 mg/KgBB. Conclusion, biologically, black grape extract can increase the expression of CD4+ and CD8+ at low doses, as well as may decrease the expression of CD4+ and CD8+ at moderate to high doses.
Jurnal Biosains Pascasarjana
Brucella Abortus infection in cattle is an infection that has a strategic effect on society, espe... more Brucella Abortus infection in cattle is an infection that has a strategic effect on society, especially the economic sector. This disease is caused by gram-negative bacteria, namely Brucella abortion. Treatment of Brucella Abortus Infection in cattle is still not effective. Therapy based on the immune response caused by Brucella Abortus Infection, especially the cytokine response, is an effective choice considering that each type of cow has a different immune response in dealing with Brucella Abortus Infection. The purpose of writing this literature review is to identify cytokines that play a role in Brucella Abortus Infection in cattle. Method: automated PUBMED database search using keywords “Brucella Abortus Bovine”, “Cytokine”, and “Brucellosis”. Results: 11 articles that are relevant and meet the inclusion criteria to analyze the cytokines that play a role in Brucella Abortus Infection in cattle. Conclusion: Cytokines that play a role in Brucella Abortus infection in cattle are ...
Jurnal Biosains Pascasarjana
Salmonella typhi is a gram-negative, intracellular facultative bacterium that can live and even r... more Salmonella typhi is a gram-negative, intracellular facultative bacterium that can live and even reproduce in macrophages, resistant to lysosomal enzymes, which has the ability to prevent phagosome-lysosome fusion, making it difficult to kill. stimulates the immune response in the host (Abbas et alll, 2016; Tores et alll, 2000). White turmeric (Curcuma zedoaria) is one of the herbal plants used as an immunomodulator. Immodulators in white turmeric substances function to increase the function of phagocytosis in macrophages to destroy Salmonella typhimurium bacteria. The purpose of this study was to determine the difference between white turmeric extract as an immunomodulator on the number of CD4+ T lymphocytes in BALB/c mice infected with Salmonella typhimurium. This research is a true experimental research with a posttest only control group design. Mice were divided into 5 groups consisting of negative control group, positive control and test group. Analysis of CD4+ lymphocyte data s...
Jurnal Biosains Pascasarjana
Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged ... more Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged hyperglycemia and elevated HbA1c levels as a standard test for diabetes mellitus. HbA1c describes the level of sugar in the blood for the last 3 months. One of the complications of diabetes mellitus is kidney damage. The state of kidney function can be described from the GFR (Glomerular Filtration Rate) examination as a standard examination for abnormalities in kidney disease. The purpose of this study was to determine the relationship between HbA1c levels and eGFR values in people with diabetes mellitus. The design of this study was a cross-sectional approach to 30 samples of DM patients who took Prolanis at the Ultra Medica Tulungagung Clinical Laboratory. The results showed that the average HbA1c level in all samples was 6.2% and the average eGFR value was 88.7 ml/min/1, 73m2. The Pearson correlation test obtained a ρ value = 0.000 so that it can be concluded that there is a relatio...
Medicra (Journal of Medical Laboratory Science/Technology), 2020
Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants... more Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 m...