Mykola Zhelavskyi - Academia.edu (original) (raw)
articles by Mykola Zhelavskyi
Scienceline Publication, 2024
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
Macropod progressive periodontal disease (MPPD), known as Lumpy Jaw, poses a persistent and poten... more Macropod progressive periodontal disease (MPPD), known as Lumpy Jaw, poses a persistent and potentially fatal threat in Western gray kangaroos when they are kept in captivity. Such a condition leads to the development of osteomyelitis and sepsis in Western gray kangaroos (Macropus fuliginosus). This case study presented the inaugural examination of hematological and biochemical aspects of MPPD with a progression toward sepsis in a captive environment. The primary objective of this research was to pinpoint hematological and biochemical indicators associated with severe MPPD in a Western gray kangaroo held in captivity. The study employed various methods, including clinical, radiographical, hematological, and biochemical analyses, as well as microbiological study methods. The case was a 2.5-year-old male wild Western gray kangaroo with fever (39.7 °C), dehydration, dyspnea, tachycardia, and involuntary jaw clenching due to stress and agitation. The kangaroo had a history of lethargy, anorexia, swelling of the soft tissues of the lower jaw on the left side, and tenderness during palpation. A radiograph of the head revealed mandible proliferative lesions. The hematological and biochemical examinations indicated an increase in the total count of leucocytes, level of neutrophils, number of erythrocytes, hematocrit level, and lymphopenia. Increased activity of alkaline phosphatase, amylase, and creatinine elevated azotemia. There was a decrease in the content of albumin, glucose, and total bilirubin. The bacteria, consisting of Fusobacteriaceae spp., Porphyromonadaceae spp., and Bacteroidaceae spp., were found and identified in all samples. However, this comprehensive diagnosis of MPPD based on clinical signs, radiography, and especially hematological and biochemical parameters of the septic process can be helpful in diagnosis and treatment.
World's Veterinary Journal, 2023
Discoid lupus erythematosus is an autoimmune disease that affects the skin in dogs. 6-year-old ma... more Discoid lupus erythematosus is an autoimmune disease that affects the skin in dogs. 6-year-old male German Shepherd weighing 38 kg was taken to the Small Animal Hospital at the University in Kyiv, Ukraine, with a history of progressive skin lesions. The indications of discoid lupus erythematosus in dogs manifested as red, scaly macules or papules on the skin's surface. These gradually develop into follicular plugging, disc-shaped plaques with adherent scales, and peripheral hyperpigmentation. The oral hydroxychloroquine was used for medication, and the prescribed dosage was 5.0 mg per kilogram of the dog's weight, administered once daily. The hydroxychloroquine was gradually reduced and discontinued within a month. Concurrent with the hydroxychloroquine treatment, the application of tacrolimus cream (Protopic ® 0.03%) was initiated. The veterinary physician also advised the dog owner to limit sun exposure to avoid any adverse effects. After a four-week period, there was a decrease in pruritus and erythema, and plaques had flattened although the skin still had some patchy hyperpigmentation. Approximately 3-4 weeks later, the veterinarian determined that the dog had achieved clinical remission as all the skin lesions had become completely flattened. The use of deproteinized calf blood extract gel (Solcoseril ® Gel for external 4.15 mg/1 g, Legacy led to the complete disappearance of the initial redness and prevented the appearance of new skin lesions. These results can be considered as a safe and effective alternative to conventional treatment methods.
Papers by Mykola Zhelavskyi
World's veterinary journal, Mar 25, 2024
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
World's Veterinary Journal, 2023
Discoid lupus erythematosus is lupus in dogs an autoimmune disease that affects the skin. A 6-yea... more Discoid lupus erythematosus is lupus in dogs an autoimmune disease that affects the skin. A 6-year-old, 38 kg, male German Shepherd dog was taken to the Small Animal Hospital at the University in Kyiv, Ukraine with a history of progressive skin lesions. The indications of discoid lupus erythematosus in dogs manifested as red, scaly macules or papules on the skin's surface. These gradually develop into follicular plugging, disc-shaped plaques with adherent scales, and peripheral hyperpigmentation. The oral hydroxychloroquine was used for medication and the prescribed dosage was 5.0 mg per kilogram of the dog's weight, administered once daily. The hydroxychloroquine was gradually reduced and discontinued within a month. Concomitant with the hydroxychloroquine treatment, the application of tacrolimus cream (Protopic® 0.03%) was initiated. The veterinary physician also advised the dog owner to limit sun exposure to avoid any adverse effects. After a four-week period, there was a...
Scienceline Publication, 2024
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
Macropod progressive periodontal disease (MPPD), known as Lumpy Jaw, poses a persistent and poten... more Macropod progressive periodontal disease (MPPD), known as Lumpy Jaw, poses a persistent and potentially fatal threat in Western gray kangaroos when they are kept in captivity. Such a condition leads to the development of osteomyelitis and sepsis in Western gray kangaroos (Macropus fuliginosus). This case study presented the inaugural examination of hematological and biochemical aspects of MPPD with a progression toward sepsis in a captive environment. The primary objective of this research was to pinpoint hematological and biochemical indicators associated with severe MPPD in a Western gray kangaroo held in captivity. The study employed various methods, including clinical, radiographical, hematological, and biochemical analyses, as well as microbiological study methods. The case was a 2.5-year-old male wild Western gray kangaroo with fever (39.7 °C), dehydration, dyspnea, tachycardia, and involuntary jaw clenching due to stress and agitation. The kangaroo had a history of lethargy, anorexia, swelling of the soft tissues of the lower jaw on the left side, and tenderness during palpation. A radiograph of the head revealed mandible proliferative lesions. The hematological and biochemical examinations indicated an increase in the total count of leucocytes, level of neutrophils, number of erythrocytes, hematocrit level, and lymphopenia. Increased activity of alkaline phosphatase, amylase, and creatinine elevated azotemia. There was a decrease in the content of albumin, glucose, and total bilirubin. The bacteria, consisting of Fusobacteriaceae spp., Porphyromonadaceae spp., and Bacteroidaceae spp., were found and identified in all samples. However, this comprehensive diagnosis of MPPD based on clinical signs, radiography, and especially hematological and biochemical parameters of the septic process can be helpful in diagnosis and treatment.
World's Veterinary Journal, 2023
Discoid lupus erythematosus is an autoimmune disease that affects the skin in dogs. 6-year-old ma... more Discoid lupus erythematosus is an autoimmune disease that affects the skin in dogs. 6-year-old male German Shepherd weighing 38 kg was taken to the Small Animal Hospital at the University in Kyiv, Ukraine, with a history of progressive skin lesions. The indications of discoid lupus erythematosus in dogs manifested as red, scaly macules or papules on the skin's surface. These gradually develop into follicular plugging, disc-shaped plaques with adherent scales, and peripheral hyperpigmentation. The oral hydroxychloroquine was used for medication, and the prescribed dosage was 5.0 mg per kilogram of the dog's weight, administered once daily. The hydroxychloroquine was gradually reduced and discontinued within a month. Concurrent with the hydroxychloroquine treatment, the application of tacrolimus cream (Protopic ® 0.03%) was initiated. The veterinary physician also advised the dog owner to limit sun exposure to avoid any adverse effects. After a four-week period, there was a decrease in pruritus and erythema, and plaques had flattened although the skin still had some patchy hyperpigmentation. Approximately 3-4 weeks later, the veterinarian determined that the dog had achieved clinical remission as all the skin lesions had become completely flattened. The use of deproteinized calf blood extract gel (Solcoseril ® Gel for external 4.15 mg/1 g, Legacy led to the complete disappearance of the initial redness and prevented the appearance of new skin lesions. These results can be considered as a safe and effective alternative to conventional treatment methods.
World's veterinary journal, Mar 25, 2024
Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, p... more Feline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×10 9 /L), their absolute content, and an increase in the percentage of neutrophils (48.2 %) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps.
World's Veterinary Journal, 2023
Discoid lupus erythematosus is lupus in dogs an autoimmune disease that affects the skin. A 6-yea... more Discoid lupus erythematosus is lupus in dogs an autoimmune disease that affects the skin. A 6-year-old, 38 kg, male German Shepherd dog was taken to the Small Animal Hospital at the University in Kyiv, Ukraine with a history of progressive skin lesions. The indications of discoid lupus erythematosus in dogs manifested as red, scaly macules or papules on the skin's surface. These gradually develop into follicular plugging, disc-shaped plaques with adherent scales, and peripheral hyperpigmentation. The oral hydroxychloroquine was used for medication and the prescribed dosage was 5.0 mg per kilogram of the dog's weight, administered once daily. The hydroxychloroquine was gradually reduced and discontinued within a month. Concomitant with the hydroxychloroquine treatment, the application of tacrolimus cream (Protopic® 0.03%) was initiated. The veterinary physician also advised the dog owner to limit sun exposure to avoid any adverse effects. After a four-week period, there was a...