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Papers by Mylarrao Bansinath

Research paper thumbnail of An in vivo method for the quantitative evaluation of local anesthetics

Journal of Pharmacological and Toxicological Methods, 2000

We describe a mouse model for evaluation of skin anesthesia after infiltration of local anestheti... more We describe a mouse model for evaluation of skin anesthesia after infiltration of local anesthetic. The method involves subcutaneous injection of the anesthetic over the abdomen, and monitoring the vocalization response to electrical stimulus as a measure of analgesia. Prior to drug injection, the vocalization threshold was determined. Mice that vocalized at 8 mA were included in the study. The model was tested using representative agents of the two classes of local anesthetics, bupivacaine, an amide, and chloroprocaine, an ester. The time course and dose response were assessed after injection. The median analgesic time was 15, 40, and 55 min for 0.015%, 0.0625%, and 0.25% bupivacaine and 30, 50, and 55 min for 0.125%, 0.25%, and 2.0% chloroprocaine, respectively. Statistical analysis of the data showed that this method is sufficiently sensitive to detect differences between the dose and duration of local anesthesia ( p < 0.05, by log rank test of the survival curves). To further validate the model, we compared the duration of anesthesia between the 0.5% bupivacaine and a new long -acting liposomal formulation of 2% bupivacaine. The results showed that the new formulation significantly prolonged the duration of anesthesia ( p < 0.05). This simple and reliable method may facilitate research on the pharmacology of infiltration anesthesia and the development of new local anesthetics and / or formulations. D

Research paper thumbnail of Reconciling Differences between In Vitro and In Vivo Effects of Propofol

Research paper thumbnail of Room E, 10/17/2000 9: 00 AM - 11: 00 AM (PS) Mitogen Activated Protein Kinase Pathway Is Involved in Morphine-Ketamine Interactions

Research paper thumbnail of Naloxone-Morphine Synergism

Anesthesia & Analgesia, 1986

Research paper thumbnail of Spinal Anesthesia by Local Anesthetics Stimulates the Enzyme Protein Kinase C and Induces the Expression of an Immediate Early Oncogene, c-Fos

Anesthesia and Analgesia, Sep 1, 1996

To understand the biochemical mechanisms involved in spinal anesthesia, we measured protein kinas... more To understand the biochemical mechanisms involved in spinal anesthesia, we measured protein kinase C (PKC) activity and expression of immediate early oncogene protein, c-Fos, in the spinal cord. Spinal anesthesia was induced in mice using intrathecal injection of either 10 PL procaine or tetracaine (0.067 M/=2%).

Research paper thumbnail of An in vivo method for the quantitative evaluation of local anesthetics

Journal of Pharmacological and Toxicological Methods, 2000

We describe a mouse model for evaluation of skin anesthesia after infiltration of local anestheti... more We describe a mouse model for evaluation of skin anesthesia after infiltration of local anesthetic. The method involves subcutaneous injection of the anesthetic over the abdomen, and monitoring the vocalization response to electrical stimulus as a measure of analgesia. Prior to drug injection, the vocalization threshold was determined. Mice that vocalized at 8 mA were included in the study. The model was tested using representative agents of the two classes of local anesthetics, bupivacaine, an amide, and chloroprocaine, an ester. The time course and dose response were assessed after injection. The median analgesic time was 15, 40, and 55 min for 0.015%, 0.0625%, and 0.25% bupivacaine and 30, 50, and 55 min for 0.125%, 0.25%, and 2.0% chloroprocaine, respectively. Statistical analysis of the data showed that this method is sufficiently sensitive to detect differences between the dose and duration of local anesthesia ( p < 0.05, by log rank test of the survival curves). To further validate the model, we compared the duration of anesthesia between the 0.5% bupivacaine and a new long -acting liposomal formulation of 2% bupivacaine. The results showed that the new formulation significantly prolonged the duration of anesthesia ( p < 0.05). This simple and reliable method may facilitate research on the pharmacology of infiltration anesthesia and the development of new local anesthetics and / or formulations. D

Research paper thumbnail of Reconciling Differences between In Vitro and In Vivo Effects of Propofol

Research paper thumbnail of Room E, 10/17/2000 9: 00 AM - 11: 00 AM (PS) Mitogen Activated Protein Kinase Pathway Is Involved in Morphine-Ketamine Interactions

Research paper thumbnail of Naloxone-Morphine Synergism

Anesthesia & Analgesia, 1986

Research paper thumbnail of Spinal Anesthesia by Local Anesthetics Stimulates the Enzyme Protein Kinase C and Induces the Expression of an Immediate Early Oncogene, c-Fos

Anesthesia and Analgesia, Sep 1, 1996

To understand the biochemical mechanisms involved in spinal anesthesia, we measured protein kinas... more To understand the biochemical mechanisms involved in spinal anesthesia, we measured protein kinase C (PKC) activity and expression of immediate early oncogene protein, c-Fos, in the spinal cord. Spinal anesthesia was induced in mice using intrathecal injection of either 10 PL procaine or tetracaine (0.067 M/=2%).