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Xanthomonas campestris pv. viticola (Xcv) e o agente causal do cancro bacteriano da videira. No S... more Xanthomonas campestris pv. viticola (Xcv) e o agente causal do cancro bacteriano da videira. No Submedio do Vale Sao Francisco, no nordeste do Brasil, o cancro bacteriano e uma das doencas mais importantes dessa cultura, sendo responsavel por grandes prejuizos, o que representa um serio risco para a viticultura brasileira. Os objetivos deste estudo foram: a) avaliar a capacidade de sete isolados de Xcv para aderir e formar biofilmes in vitro e determinar a influencia do meio de cultura e das superficies na formacao de biofilme, alem de investigar a arquitetura do biofilme e motilidade swarming; e b) otimizar um protocolo para extracao de proteinas para analise da proteomica de Xcv, comparando a eficiencia de quatro metodos com base no perfil de eletroforese em gel bidimensional (2D-PAGE). Todos os isolados aderiram aos pocos da placa de microtitulacao de poliestireno e formaram biofilmes nos meios de cultura liquidos testados (caldo nutriente-dextrose-extrato de levedura, extrato de...

Revista em Agronegócio e Meio Ambiente, 2019

O objetivo desse trabalho foi avaliar seis genótipos de milho com características promissoras par... more O objetivo desse trabalho foi avaliar seis genótipos de milho com características promissoras para a produção de minimilho e adaptados à região da Zona da Mata de Pernambuco, região Nordeste do Brasil. O experimento foi conduzido no campus experimental da Fitotecnia na UFRPE, entre 2014/2015. Foram avaliados seis genótipos, quanto a dez variáveis agronômicas. Na análise de variância pode ser observado que as variáveis significativas para produção do minimilho foram: número de espigas, início para a diferenciação da panícula, altura da planta aos 30 dias após a emergência, peso da espiga e peso da parte aérea. As correlações genotípicas foram mais elevadas do que as fenotípicas, porém de mesmo sinal, indicando menor influência do ambiente na expressão dos caracteres. Na análise de trilha ficou demonstrado que mais de 96% (R2) de influência das variáveis sobre número de espigas e com um baixo efeito residual 0,17, confirmando que as variáveis analisadas influenciam na produção do núme...

A couve-chinesa (Brassica pekinensis L.) destaca-se na olericultura brasileira pelo valor nutrici... more A couve-chinesa (Brassica pekinensis L.) destaca-se na olericultura brasileira pelo valor nutricional e aumento da produtividade. Nas regioes da Zona da Mata e do Agreste Pernambucano esta hortalica e intensamente cultivada no sistema convencional tendo sua producao limitada entre outros fatores pela ocorrencia da podridao mole. Avaliou-se a acao de oleos essenciais no controle da podridao mole em couve-chinesa e sua influencia na colorimetria e caracteristicas fisico-quimicas da hortalica. Em testes preliminares de fitotoxidez foram selecionados 11 oleos. Em casa de vegetacao, plantas da cv. Natsume foram pulverizadas com os oleos de bergamota, capim limao, copaiba, eucalipto citriodora, eucalipto globulus, funcho de erva-doce, gengibre, hortela, laranja doce, limao e salvia esclareia (0,5%) e o antibiotico agricola Mycoshield® (3g L-1), sendo inoculadas com Pectobacterium carotovorum subsp. carotovorum (Pcc-c) apos 72 h. Avaliou-se a severidade da doenca a cada seis horas ate 48 h...

Journal of Environmental Analysis and Progress, 2018

Phytopathogenic bacteria are responsible for causing several losses in agriculture economic world... more Phytopathogenic bacteria are responsible for causing several losses in agriculture economic world. Biofilm formation presents itself as an important structure related to bacterial virulence. The objective of this study was to investigate the biofilm formation by Ralstonia solanacearum and Acidovorax citrulli isolates. Quantification of biofilm formation was performed by the crystal violet method, using NYD as the standard medium for both bacteria, TZC as a specific medium for R. solanacearum and YDC for A. citrulli. The biofilm was observed in SEM and CLSM. Under the tested conditions, B5-5, CGH8, CGM10 and CGH26 R. solanacearum isolates and Aac1.43 and Aac1.73 A. citrulli isolate formed moderately or strongly biofilm on both media tested. However, the amount of biofilm produced by R. solanacearum was higher than those produced by A. citrulli. The SEM and CLSM revealed structurally distinct biofilms among isolates of R. solanacearum, which did not occur for A. citrulli isolates. We ...

Tropical Plant Pathology, 2017

Biofilms are dense surface-associated communities formed by microorganisms. Formation of these st... more Biofilms are dense surface-associated communities formed by microorganisms. Formation of these structures by the plant pathogenic bacterium Xanthomonas campestris pv. viticola (bacterial canker of grapevine) had not previously been studied. The ability of seven strains of this bacterium to adhere to abiotic surfaces and to form biofilms in vitro was evaluated under different conditions. The surfaces tested were polystyrene and glass using microtiter plates and tubes, respectively. Four liquid culture media were used, nutrientdextrose-yeast extract (NYD), yeast extract-dextrose-calcium carbonate (YDC), Kado 523 (KADO) and Luria-Bertani (LB). The biofilm architecture was examined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Seven strains adhered to polystyrene in the microtiter plates and formed biofilms in all culture media at weak, moderate, and strong levels. In glass tubes, only strains Xcv229 and Xcv158 formed biofilms. SEM of Xcv229 and Xcv158 revealed typical biofilm architectures. CLSM showed that only Xcv229 formed an initial matrix structure characteristic of biofilms. The X. campestris pv. viticola strains exhibited different levels of biofilm formation in different culture media, of which LB and KADO were the best. Therefore, bacterial growth in polystyrene microtiter plates using LB and KADO media is a good qualitative method for the detection of biofilms of this pathogen. Keywords Vitis vinifera. Bacterial canker disease. CLSM. SEM Xanthomonas campestris pv. viticola (Nayudu) Dye is the causal agent of bacterial canker of grapevine, which is the main grapevine bacteriosis in irrigated areas of the São Francisco river in the Pernambuco and Bahia states located in the Brazilian northeastern region. These states account for 99% of annual exports of the Brazilian table grape to Holland, Germany, England, France, Spain and the United States (Lazzarotto and Fioravanço 2013). Because there is no single most efficient method for the control of bacterial canker of grapevine, several preventive and eradication measures are recommended, making the disease control more difficult. This is mainly due to the ability of the bacterium to survive in infected plants, in symptomless plants as an epiphyte (Araujo 2001), in crop debris (Silva et al. 2012), in alternative hosts (Peixoto et al. 2007), in grapevine cutting tools and in water (Naue et al. 2014). Consequently, the disease continues to constrain the productivity of Valley vineyards, limit the sale of grapes in both national and international markets, and prevent the transfer of grapevine propagating material from the Brazilian states where the bacterium has been detected to other areas (Naue et al. 2014). Due to the economic damage caused by bacterial canker of grapevine, there has been a major effort to generate more information about X. campestris pv. viticola. Several bacterial species that affect different host plants and cause diverse disease phenotypes have the ability to adhere and form biofilms (Lorite et al. 2013; Zimaro et al. 2013;

Revista Ciência Agronômica, 2014

Óleos essenciais vegetais para a redução da intensidade da podridão mole em couvechinesa

African Journal of Biotechnology, Aug 20, 2014

Two-dim v. vitic Myrzânia de Ram epartamento Departamento An efficien proteomics phytopatho the p... more Two-dim v. vitic Myrzânia de Ram epartamento Departamento An efficien proteomics phytopatho the phytob compared, centrifugati suitable me a significan concentrati larger numb quality of th option for t

Proteome Science, 2015

Background: Finding the best extraction method of proteins from lysed cells is the key step for d... more Background: Finding the best extraction method of proteins from lysed cells is the key step for detection and identification in all proteomics applications. These are important to complement the knowledge about the mechanisms of interaction between plants and phytopathogens causing major economic losses. To develop an optimized extraction protocol, strains of Acidovorax citrulli, Pectobacterium carotovorum subsp. carotovorum and Ralstonia solanacearum were used as representative cells in the study of phytopathogenic bacteria. This study aims to compare four different protein extraction methods, including: Trizol, Phenol, Centrifugation and Lysis in order to determine which are more suitable for proteomic studies using as parameters the quantity and quality of extracted proteins observed in two-dimensional gels. Results: The bacteria studied showed different results among the tested methods. The Lysis method was more efficient for P. carotovorum subsp. carotovorum and R. solanacearum phytobacteria, as well as simple and fast, while for A. citrulli, the Centrifugation method was the best. This evaluation is based on results obtained in polyacrylamide gels that presented a greater abundance of spots and clearer and more consistent strips as detected by two-dimensional gels. Conclusions: These results attest to the adequacy of these proteins extraction methods for proteomic studies.

Xanthomonas campestris pv. viticola (Xcv) e o agente causal do cancro bacteriano da videira. No S... more Xanthomonas campestris pv. viticola (Xcv) e o agente causal do cancro bacteriano da videira. No Submedio do Vale Sao Francisco, no nordeste do Brasil, o cancro bacteriano e uma das doencas mais importantes dessa cultura, sendo responsavel por grandes prejuizos, o que representa um serio risco para a viticultura brasileira. Os objetivos deste estudo foram: a) avaliar a capacidade de sete isolados de Xcv para aderir e formar biofilmes in vitro e determinar a influencia do meio de cultura e das superficies na formacao de biofilme, alem de investigar a arquitetura do biofilme e motilidade swarming; e b) otimizar um protocolo para extracao de proteinas para analise da proteomica de Xcv, comparando a eficiencia de quatro metodos com base no perfil de eletroforese em gel bidimensional (2D-PAGE). Todos os isolados aderiram aos pocos da placa de microtitulacao de poliestireno e formaram biofilmes nos meios de cultura liquidos testados (caldo nutriente-dextrose-extrato de levedura, extrato de...

Revista em Agronegócio e Meio Ambiente, 2019

O objetivo desse trabalho foi avaliar seis genótipos de milho com características promissoras par... more O objetivo desse trabalho foi avaliar seis genótipos de milho com características promissoras para a produção de minimilho e adaptados à região da Zona da Mata de Pernambuco, região Nordeste do Brasil. O experimento foi conduzido no campus experimental da Fitotecnia na UFRPE, entre 2014/2015. Foram avaliados seis genótipos, quanto a dez variáveis agronômicas. Na análise de variância pode ser observado que as variáveis significativas para produção do minimilho foram: número de espigas, início para a diferenciação da panícula, altura da planta aos 30 dias após a emergência, peso da espiga e peso da parte aérea. As correlações genotípicas foram mais elevadas do que as fenotípicas, porém de mesmo sinal, indicando menor influência do ambiente na expressão dos caracteres. Na análise de trilha ficou demonstrado que mais de 96% (R2) de influência das variáveis sobre número de espigas e com um baixo efeito residual 0,17, confirmando que as variáveis analisadas influenciam na produção do núme...

A couve-chinesa (Brassica pekinensis L.) destaca-se na olericultura brasileira pelo valor nutrici... more A couve-chinesa (Brassica pekinensis L.) destaca-se na olericultura brasileira pelo valor nutricional e aumento da produtividade. Nas regioes da Zona da Mata e do Agreste Pernambucano esta hortalica e intensamente cultivada no sistema convencional tendo sua producao limitada entre outros fatores pela ocorrencia da podridao mole. Avaliou-se a acao de oleos essenciais no controle da podridao mole em couve-chinesa e sua influencia na colorimetria e caracteristicas fisico-quimicas da hortalica. Em testes preliminares de fitotoxidez foram selecionados 11 oleos. Em casa de vegetacao, plantas da cv. Natsume foram pulverizadas com os oleos de bergamota, capim limao, copaiba, eucalipto citriodora, eucalipto globulus, funcho de erva-doce, gengibre, hortela, laranja doce, limao e salvia esclareia (0,5%) e o antibiotico agricola Mycoshield® (3g L-1), sendo inoculadas com Pectobacterium carotovorum subsp. carotovorum (Pcc-c) apos 72 h. Avaliou-se a severidade da doenca a cada seis horas ate 48 h...

Journal of Environmental Analysis and Progress, 2018

Phytopathogenic bacteria are responsible for causing several losses in agriculture economic world... more Phytopathogenic bacteria are responsible for causing several losses in agriculture economic world. Biofilm formation presents itself as an important structure related to bacterial virulence. The objective of this study was to investigate the biofilm formation by Ralstonia solanacearum and Acidovorax citrulli isolates. Quantification of biofilm formation was performed by the crystal violet method, using NYD as the standard medium for both bacteria, TZC as a specific medium for R. solanacearum and YDC for A. citrulli. The biofilm was observed in SEM and CLSM. Under the tested conditions, B5-5, CGH8, CGM10 and CGH26 R. solanacearum isolates and Aac1.43 and Aac1.73 A. citrulli isolate formed moderately or strongly biofilm on both media tested. However, the amount of biofilm produced by R. solanacearum was higher than those produced by A. citrulli. The SEM and CLSM revealed structurally distinct biofilms among isolates of R. solanacearum, which did not occur for A. citrulli isolates. We ...

Tropical Plant Pathology, 2017

Biofilms are dense surface-associated communities formed by microorganisms. Formation of these st... more Biofilms are dense surface-associated communities formed by microorganisms. Formation of these structures by the plant pathogenic bacterium Xanthomonas campestris pv. viticola (bacterial canker of grapevine) had not previously been studied. The ability of seven strains of this bacterium to adhere to abiotic surfaces and to form biofilms in vitro was evaluated under different conditions. The surfaces tested were polystyrene and glass using microtiter plates and tubes, respectively. Four liquid culture media were used, nutrientdextrose-yeast extract (NYD), yeast extract-dextrose-calcium carbonate (YDC), Kado 523 (KADO) and Luria-Bertani (LB). The biofilm architecture was examined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Seven strains adhered to polystyrene in the microtiter plates and formed biofilms in all culture media at weak, moderate, and strong levels. In glass tubes, only strains Xcv229 and Xcv158 formed biofilms. SEM of Xcv229 and Xcv158 revealed typical biofilm architectures. CLSM showed that only Xcv229 formed an initial matrix structure characteristic of biofilms. The X. campestris pv. viticola strains exhibited different levels of biofilm formation in different culture media, of which LB and KADO were the best. Therefore, bacterial growth in polystyrene microtiter plates using LB and KADO media is a good qualitative method for the detection of biofilms of this pathogen. Keywords Vitis vinifera. Bacterial canker disease. CLSM. SEM Xanthomonas campestris pv. viticola (Nayudu) Dye is the causal agent of bacterial canker of grapevine, which is the main grapevine bacteriosis in irrigated areas of the São Francisco river in the Pernambuco and Bahia states located in the Brazilian northeastern region. These states account for 99% of annual exports of the Brazilian table grape to Holland, Germany, England, France, Spain and the United States (Lazzarotto and Fioravanço 2013). Because there is no single most efficient method for the control of bacterial canker of grapevine, several preventive and eradication measures are recommended, making the disease control more difficult. This is mainly due to the ability of the bacterium to survive in infected plants, in symptomless plants as an epiphyte (Araujo 2001), in crop debris (Silva et al. 2012), in alternative hosts (Peixoto et al. 2007), in grapevine cutting tools and in water (Naue et al. 2014). Consequently, the disease continues to constrain the productivity of Valley vineyards, limit the sale of grapes in both national and international markets, and prevent the transfer of grapevine propagating material from the Brazilian states where the bacterium has been detected to other areas (Naue et al. 2014). Due to the economic damage caused by bacterial canker of grapevine, there has been a major effort to generate more information about X. campestris pv. viticola. Several bacterial species that affect different host plants and cause diverse disease phenotypes have the ability to adhere and form biofilms (Lorite et al. 2013; Zimaro et al. 2013;

Revista Ciência Agronômica, 2014

Óleos essenciais vegetais para a redução da intensidade da podridão mole em couvechinesa

African Journal of Biotechnology, Aug 20, 2014

Two-dim v. vitic Myrzânia de Ram epartamento Departamento An efficien proteomics phytopatho the p... more Two-dim v. vitic Myrzânia de Ram epartamento Departamento An efficien proteomics phytopatho the phytob compared, centrifugati suitable me a significan concentrati larger numb quality of th option for t

Proteome Science, 2015

Background: Finding the best extraction method of proteins from lysed cells is the key step for d... more Background: Finding the best extraction method of proteins from lysed cells is the key step for detection and identification in all proteomics applications. These are important to complement the knowledge about the mechanisms of interaction between plants and phytopathogens causing major economic losses. To develop an optimized extraction protocol, strains of Acidovorax citrulli, Pectobacterium carotovorum subsp. carotovorum and Ralstonia solanacearum were used as representative cells in the study of phytopathogenic bacteria. This study aims to compare four different protein extraction methods, including: Trizol, Phenol, Centrifugation and Lysis in order to determine which are more suitable for proteomic studies using as parameters the quantity and quality of extracted proteins observed in two-dimensional gels. Results: The bacteria studied showed different results among the tested methods. The Lysis method was more efficient for P. carotovorum subsp. carotovorum and R. solanacearum phytobacteria, as well as simple and fast, while for A. citrulli, the Centrifugation method was the best. This evaluation is based on results obtained in polyacrylamide gels that presented a greater abundance of spots and clearer and more consistent strips as detected by two-dimensional gels. Conclusions: These results attest to the adequacy of these proteins extraction methods for proteomic studies.