JU NA - Academia.edu (original) (raw)

Papers by JU NA

Pulmonary Pharmacology & Therapeutics, 2006

Study objective: To study whether there is any difference in thfe clinical characteristics betwee... more Study objective: To study whether there is any difference in thfe clinical characteristics between the two patterns of perfusion lung scan of idiopathic pulmonary arterial hypertension (normal vs. diffuse, multiple ill-defined defects) and whether the perfusion lung scan patterns of these patients would predict the effect of long-term use of beraprost sodium. Methods: We evaluated 27 patients who used beraprost sodium for over 3 months, and noted a diffuse patchy pattern in 13 cases and a normal pattern in the remaining 14 cases. We judged that beraprost sodium was effective when at least two of the following conditions were met: improvement in symptom of dyspnea, more than 10% decrease in peak velocity of tricuspid valve regurgitation by echocardiography (V max ), or more than 10% increase in 6-min walking distance. Results: At baseline there was no difference between the two groups in dyspnea, hemodynamic parameters, and 6-min walking distance. After the use of beraprost sodium, the normal group showed improvement in dyspnea, 6-min walking distance, and V max . But the diffuse patchy group showed no improvement. The use of beraprost sodium in the normal group was effective in 10 out of 14 cases, but was effective in only two out of 13 cases in the diffuse patchy group. Conclusion: Perfusion lung scan pattern in patients with idiopathic pulmonary arterial hypertension is a useful prognostic indicator of the response to beraprost sodium. q Abbreviations IPAH, idiopathic pulmonary arterial hypertension; NYHA, New York Heart Association; V max , peak velocity through tricuspid valve; MPAP, mean pulmonary arterial pressure; PVR, pulmonary vascular resistance; PCW, pulmonary capillary wedge pressure; CO, cardiac output; CI, cardiac; S V O 2 , mixed venous oxygen saturation.

International Journal of Systematic and Evolutionary Microbiology, 2007

A Gram-positive, non-spore-forming, rod-shaped and non-motile bacterium, strain Gsoil 355 T , was... more A Gram-positive, non-spore-forming, rod-shaped and non-motile bacterium, strain Gsoil 355 T , was isolated from soil of a ginseng field in South Korea. In phylogenetic analyses based on 16S rRNA gene sequences, strain Gsoil 355 T showed the highest levels of sequence similarity with respect to Solirubrobacter pauli B33D1 T (97.4 %), Conexibacter woesei DSM 14684 T (94.2 %) and Patulibacter minatonensis KV-614 T (91.8 %). The strain possesses menaquinone MK-7(H 4 ) and contains C 16 : 0 and C 18 : 0 v9c as the predominant fatty acids. The DNA G+C content is 71.5 mol%. On the basis of genotypic and phenotypic characteristics, strain Gsoil 355 T represents a novel species of the genus Solirubrobacter, for which the name Solirubrobacter soli sp. nov. is proposed. The type strain is Gsoil 355 T (=KCTC 12628 T =LMG 23485 T ).

International Journal of Systematic and Evolutionary Microbiology, 2007

Strain Jip14 T , a Gram-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolat... more Strain Jip14 T , a Gram-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from dried rice straw and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Jip14 T belongs to the family Sphingobacteriaceae, and the highest degree of sequence similarity was determined to be to Pedobacter saltans DSM 12145 T (88.5 %), Pedobacter africanus DSM 12126 T (87.6 %), Pedobacter heparinus DSM 2366 T (87.1 %) and Pedobacter caeni LMG 22862 T (86.9 %). Chemotaxonomic data revealed that strain Jip14 T possesses menaquinone MK-7 and the predominant fatty acids C 15 : 0 iso, C 16 : 0 , C 16 : 0 10-methyl, C 17 : 0 iso 3-OH and summed feature 3 (C 15 : 0 iso 2-OH/C 16 : 1 v7c). The results of physiological and biochemical tests clearly demonstrated that strain Jip14 T represents a distinct species. Based on these data, Jip14 T should be classified within a novel genus and species, for which the name Parapedobacter koreensis gen. nov., sp. nov. is proposed. The type strain of Parapedobacter koreensis is Jip14 T (=KCTC 12643 T =LMG 23493 T ).

International Journal of Systematic and Evolutionary Microbiology, 2007

Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga-Flexibacter-Bacte... more Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga-Flexibacter-Bacteroides (CFB) group], designated Gsoil 040 T and Gsoil 052 T , were isolated from the soil of a ginseng field in Pocheon province, South Korea. A polyphasic approach was used to characterize the taxonomic position of the novel strains. Both strains were Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Chitinophaga but are clearly separated from the recognized species of this genus; gene sequence similarities between the novel isolates and type strains of recognized species ranged from 91.2 to 96.5 %. One exception was found; strain Gsoil 052 T and the type strain of Chitinophaga filiformis had a gene sequence similarity of 99.6 % but had a DNA-DNA relatedness value of 38 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C 15 : 0 and C 16 : 1 v5c; major hydroxy fatty acid, iso-C 17 : 0 3-OH and major polyamine, homospermidine) supported the affiliation of both strains Gsoil 040 T and Gsoil 052 T to the genus Chitinophaga. The results of physiological and biochemical tests enabled the genotypic and phenotypic differentiation of the novel strains from the other recognized species of the genus Chitinophaga. Therefore, it is suggested that the new isolates represent two novel species, for which the names Chitinophaga ginsengisegetis sp. nov. [type strain Gsoil 040 T (=KCTC 12654 T =DSM 18108 T )] and Chitinophaga ginsengisoli sp. nov. [type strain Gsoil 052 T (=KCTC 12592 T =DSM 18017 T )] are proposed.

Phytochemistry, 2008

Ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological ... more Ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological activities. Many studies have been directed towards converting major ginsenosides to the more active minor ginsenoside, Rg3. Due to the difficulty in preparing ginsenoside Rg3 enzymatically, the compound has been mainly produced by either acid treatment or heating. A microbial strain GS514 was isolated from soil around ginseng roots in a field and used for enzymatic preparation of the ginsenoside Rg3. Blast results of the 16S rRNA gene sequence of the strain GS514 established that the strain GS514 belonged to the genus Microbacterium. Its 16S rRNA gene sequence showed 98.7%, 98.4% and 96.1% identity with those of M. esteraromaticum, M. arabinogalactanolyticum and M. lacticum. Strain GS514 showed a strong ability to convert ginsenoside Rb1 or Rd into Rg3. Enzymatic production of Rg3 occurred by consecutive hydrolyses of the terminal and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1 showing the biotransformation pathway: Rb1 ! Rd ! Rg3.

Association of http://www.chestjournal.org/content/135/5/1173.full.html and services can be found... more Association of http://www.chestjournal.org/content/135/5/1173.full.html and services can be found online on the World Wide Web at: The online version of this article, along with updated information ) ISSN:0012-3692 Background: Interleukin (IL)-17E is a member of the IL-17 family, which induces IL-4, IL-5, IL-13, and eotaxin in experimental animals via IL-17 receptor B (IL-17RB). The activation of IL-17RB amplifies allergic-type inflammatory responses by inducing Jun kinase (or JNK), p38 mitogen-activated protein kinase (or MAPK), and nuclear factor-B. Objectives: We examined the association of polymorphisms in the IL-17RB gene with asthma susceptibility and investigated the effects of those polymorphisms on the transcription of various IL-17RB isoforms. Methods: In total, 954 asthmatic patients or 265 healthy control subjects were screened for polymorphisms in IL-17RB by single-base extension. The messenger RNA expression IL-17RB in B-cell lines derived from patients was also measured by reverse transcription-polymerase chain reaction. Results: Direct sequencing of 24 unrelated Korean DNA samples revealed 18 genetic variants, including four insertion/deletions and 14 single-nucleotide polymorphisms (SNPs). Six of the SNPs (؊1465G>A, ؉5661G>A, ؉6297T>C ͓Y123Y͔, ؉13797C>T, ؉18661C>T, and ؉18965G>A) were used to screen a larger group of subjects. Intronic polymorphism ؉5661G>A was significantly associated with the development of asthma (p ‫؍‬ 0.001); moreover, a minor allele of IL-17RB ؉5661G>A appeared at a lower frequency in the asthmatic patients than in the healthy control subjects (0.13 vs 0.19, respectively). The IL-17RB messenger RNA expression in B cells homozygous for IL-17RB؉ 5661GG was significantly higher than that in B cells homozygous for IL-17RB؉5661AA (p ‫؍‬ 0.002). Conclusions: A rare allele of IL-17RB ؉5661G>A may have a protective role against the development of asthma via regulation at the level of transcription. The SNPs identified in this study may be used to develop markers to assess the risk of asthma. (CHEST 2009; 135:1173-1180)

Tuberculosis and Respiratory Diseases, 2008

Abstract It is difficult to distinguish a lung cancer from a pulmonary tuberculoma or other benig... more Abstract It is difficult to distinguish a lung cancer from a pulmonary tuberculoma or other benign nodule. It is even more difficult to identify the type of lesion if the mass shows no change in size or demonstrates slow growth. Only a pathological confirmation can ...

Pulmonary Pharmacology & Therapeutics, 2006

Study objective: To study whether there is any difference in thfe clinical characteristics betwee... more Study objective: To study whether there is any difference in thfe clinical characteristics between the two patterns of perfusion lung scan of idiopathic pulmonary arterial hypertension (normal vs. diffuse, multiple ill-defined defects) and whether the perfusion lung scan patterns of these patients would predict the effect of long-term use of beraprost sodium. Methods: We evaluated 27 patients who used beraprost sodium for over 3 months, and noted a diffuse patchy pattern in 13 cases and a normal pattern in the remaining 14 cases. We judged that beraprost sodium was effective when at least two of the following conditions were met: improvement in symptom of dyspnea, more than 10% decrease in peak velocity of tricuspid valve regurgitation by echocardiography (V max ), or more than 10% increase in 6-min walking distance. Results: At baseline there was no difference between the two groups in dyspnea, hemodynamic parameters, and 6-min walking distance. After the use of beraprost sodium, the normal group showed improvement in dyspnea, 6-min walking distance, and V max . But the diffuse patchy group showed no improvement. The use of beraprost sodium in the normal group was effective in 10 out of 14 cases, but was effective in only two out of 13 cases in the diffuse patchy group. Conclusion: Perfusion lung scan pattern in patients with idiopathic pulmonary arterial hypertension is a useful prognostic indicator of the response to beraprost sodium. q Abbreviations IPAH, idiopathic pulmonary arterial hypertension; NYHA, New York Heart Association; V max , peak velocity through tricuspid valve; MPAP, mean pulmonary arterial pressure; PVR, pulmonary vascular resistance; PCW, pulmonary capillary wedge pressure; CO, cardiac output; CI, cardiac; S V O 2 , mixed venous oxygen saturation.

International Journal of Systematic and Evolutionary Microbiology, 2007

A Gram-positive, non-spore-forming, rod-shaped and non-motile bacterium, strain Gsoil 355 T , was... more A Gram-positive, non-spore-forming, rod-shaped and non-motile bacterium, strain Gsoil 355 T , was isolated from soil of a ginseng field in South Korea. In phylogenetic analyses based on 16S rRNA gene sequences, strain Gsoil 355 T showed the highest levels of sequence similarity with respect to Solirubrobacter pauli B33D1 T (97.4 %), Conexibacter woesei DSM 14684 T (94.2 %) and Patulibacter minatonensis KV-614 T (91.8 %). The strain possesses menaquinone MK-7(H 4 ) and contains C 16 : 0 and C 18 : 0 v9c as the predominant fatty acids. The DNA G+C content is 71.5 mol%. On the basis of genotypic and phenotypic characteristics, strain Gsoil 355 T represents a novel species of the genus Solirubrobacter, for which the name Solirubrobacter soli sp. nov. is proposed. The type strain is Gsoil 355 T (=KCTC 12628 T =LMG 23485 T ).

International Journal of Systematic and Evolutionary Microbiology, 2007

Strain Jip14 T , a Gram-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolat... more Strain Jip14 T , a Gram-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from dried rice straw and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Jip14 T belongs to the family Sphingobacteriaceae, and the highest degree of sequence similarity was determined to be to Pedobacter saltans DSM 12145 T (88.5 %), Pedobacter africanus DSM 12126 T (87.6 %), Pedobacter heparinus DSM 2366 T (87.1 %) and Pedobacter caeni LMG 22862 T (86.9 %). Chemotaxonomic data revealed that strain Jip14 T possesses menaquinone MK-7 and the predominant fatty acids C 15 : 0 iso, C 16 : 0 , C 16 : 0 10-methyl, C 17 : 0 iso 3-OH and summed feature 3 (C 15 : 0 iso 2-OH/C 16 : 1 v7c). The results of physiological and biochemical tests clearly demonstrated that strain Jip14 T represents a distinct species. Based on these data, Jip14 T should be classified within a novel genus and species, for which the name Parapedobacter koreensis gen. nov., sp. nov. is proposed. The type strain of Parapedobacter koreensis is Jip14 T (=KCTC 12643 T =LMG 23493 T ).

International Journal of Systematic and Evolutionary Microbiology, 2007

Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga-Flexibacter-Bacte... more Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga-Flexibacter-Bacteroides (CFB) group], designated Gsoil 040 T and Gsoil 052 T , were isolated from the soil of a ginseng field in Pocheon province, South Korea. A polyphasic approach was used to characterize the taxonomic position of the novel strains. Both strains were Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Chitinophaga but are clearly separated from the recognized species of this genus; gene sequence similarities between the novel isolates and type strains of recognized species ranged from 91.2 to 96.5 %. One exception was found; strain Gsoil 052 T and the type strain of Chitinophaga filiformis had a gene sequence similarity of 99.6 % but had a DNA-DNA relatedness value of 38 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C 15 : 0 and C 16 : 1 v5c; major hydroxy fatty acid, iso-C 17 : 0 3-OH and major polyamine, homospermidine) supported the affiliation of both strains Gsoil 040 T and Gsoil 052 T to the genus Chitinophaga. The results of physiological and biochemical tests enabled the genotypic and phenotypic differentiation of the novel strains from the other recognized species of the genus Chitinophaga. Therefore, it is suggested that the new isolates represent two novel species, for which the names Chitinophaga ginsengisegetis sp. nov. [type strain Gsoil 040 T (=KCTC 12654 T =DSM 18108 T )] and Chitinophaga ginsengisoli sp. nov. [type strain Gsoil 052 T (=KCTC 12592 T =DSM 18017 T )] are proposed.

Phytochemistry, 2008

Ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological ... more Ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological activities. Many studies have been directed towards converting major ginsenosides to the more active minor ginsenoside, Rg3. Due to the difficulty in preparing ginsenoside Rg3 enzymatically, the compound has been mainly produced by either acid treatment or heating. A microbial strain GS514 was isolated from soil around ginseng roots in a field and used for enzymatic preparation of the ginsenoside Rg3. Blast results of the 16S rRNA gene sequence of the strain GS514 established that the strain GS514 belonged to the genus Microbacterium. Its 16S rRNA gene sequence showed 98.7%, 98.4% and 96.1% identity with those of M. esteraromaticum, M. arabinogalactanolyticum and M. lacticum. Strain GS514 showed a strong ability to convert ginsenoside Rb1 or Rd into Rg3. Enzymatic production of Rg3 occurred by consecutive hydrolyses of the terminal and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1 showing the biotransformation pathway: Rb1 ! Rd ! Rg3.

Association of http://www.chestjournal.org/content/135/5/1173.full.html and services can be found... more Association of http://www.chestjournal.org/content/135/5/1173.full.html and services can be found online on the World Wide Web at: The online version of this article, along with updated information ) ISSN:0012-3692 Background: Interleukin (IL)-17E is a member of the IL-17 family, which induces IL-4, IL-5, IL-13, and eotaxin in experimental animals via IL-17 receptor B (IL-17RB). The activation of IL-17RB amplifies allergic-type inflammatory responses by inducing Jun kinase (or JNK), p38 mitogen-activated protein kinase (or MAPK), and nuclear factor-B. Objectives: We examined the association of polymorphisms in the IL-17RB gene with asthma susceptibility and investigated the effects of those polymorphisms on the transcription of various IL-17RB isoforms. Methods: In total, 954 asthmatic patients or 265 healthy control subjects were screened for polymorphisms in IL-17RB by single-base extension. The messenger RNA expression IL-17RB in B-cell lines derived from patients was also measured by reverse transcription-polymerase chain reaction. Results: Direct sequencing of 24 unrelated Korean DNA samples revealed 18 genetic variants, including four insertion/deletions and 14 single-nucleotide polymorphisms (SNPs). Six of the SNPs (؊1465G>A, ؉5661G>A, ؉6297T>C ͓Y123Y͔, ؉13797C>T, ؉18661C>T, and ؉18965G>A) were used to screen a larger group of subjects. Intronic polymorphism ؉5661G>A was significantly associated with the development of asthma (p ‫؍‬ 0.001); moreover, a minor allele of IL-17RB ؉5661G>A appeared at a lower frequency in the asthmatic patients than in the healthy control subjects (0.13 vs 0.19, respectively). The IL-17RB messenger RNA expression in B cells homozygous for IL-17RB؉ 5661GG was significantly higher than that in B cells homozygous for IL-17RB؉5661AA (p ‫؍‬ 0.002). Conclusions: A rare allele of IL-17RB ؉5661G>A may have a protective role against the development of asthma via regulation at the level of transcription. The SNPs identified in this study may be used to develop markers to assess the risk of asthma. (CHEST 2009; 135:1173-1180)

Tuberculosis and Respiratory Diseases, 2008

Abstract It is difficult to distinguish a lung cancer from a pulmonary tuberculoma or other benig... more Abstract It is difficult to distinguish a lung cancer from a pulmonary tuberculoma or other benign nodule. It is even more difficult to identify the type of lesion if the mass shows no change in size or demonstrates slow growth. Only a pathological confirmation can ...