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Research paper thumbnail of Indicadores de salud perinatal: diferencias entre la información registrada por el INE y la de los hospitales donde se atienden los nacimientos

Revista Española de Salud Pública, 2015

Ariadna Ayerza Casas (1,2) and Néstor Herraiz Esteban (3).

Research paper thumbnail of La rata Wistar como modelo animal experimental, para el adiestramiento laparoscópico de residentes con entrenadores pélvicos

Progresos de Obstetricia y Ginecología, 2011

Entrenador pélvico ginecológico; Rata Wistar; Ejercicios de entrenamiento; Endoscopia ginecológic... more Entrenador pélvico ginecológico; Rata Wistar; Ejercicios de entrenamiento; Endoscopia ginecológica Resumen Presentamos nuevos ejercicios de entrenamiento endoscópico ginecológico en modelo animal (rata Wistar) para formación de los residentes en esta disciplina quirúrgica. Las intervenciones y los ejercicios de adiestramiento se efectuarán en una rata Wistar viva y anestesiada, introducida en un entrenador pélvico endoscópico ginecológico. A través del mismo se realizarán en la rata las prácticas de disección, coagulación, corte, movilización de estructuras de cavidad abdominal, histerectomía subtotal con anexectomía bilateral, extracción de pieza en bolsa y anudado para cerrar la herida abdominal. Con 12 h de trabajo un residente de obstetricia y ginecología en su tercer año conseguiría el dominio de los movimientos necesarios para realizarlos en humanos.

Research paper thumbnail of Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR

Research in Veterinary Science, 2013

Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene... more Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele *1501 has been associated with high proviral load in peripheral blood while allele *0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB3*0902) or susceptibility (BoLA DRB3*1501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR® Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle.

Research paper thumbnail of A rapid and sensitive method of identification of HTLV-II subtypes

Journal of Medical Virology, 1995

There are 2 subtypes of human T-cell lymphoma/ leukemia virus type II (HTLV-11), A and B. HTLV-II... more There are 2 subtypes of human T-cell lymphoma/ leukemia virus type II (HTLV-11), A and B. HTLV-II is increasingly associated with rare forms of lymphocytic neoplasia and a neurodegenerative disorder, characterized by hyperspasticity and ataxia. We have used PCR to amplify, clone and sequence 140 bp of the pol gene from many isolates of HTLV-IIA and HTLV-IIB from around the world. Analysis of these and other published sequences established that all HTLV-IIA sequences contained a unique Hinf I site and all HTLV-IIB sequences a unique Mse I site. A rapid and specific oligomer restriction (OR) assay was developed utilizing the primer pair SKI 10/SK111 and subsequent digestion with these enzymes. Concordance between sequenced and OR-based subtyping of DNA amplified by PCR was absolute among 22 HTLV-II isolates tested. Further OR or sequence analyses on an additional 30 other isolates indicated that the majority of North American non-indian HTLV-I1 isolates were subtype A, while all Paleo-Amerindian samples, including those from the Seminole of Florida; the Guaymi from Panama; and the Toba, Chorote, Wichi, and Chulupe of Argentina, belonged to subtype B. The SKIIO/SKI 11 PCR-OR format should facilitate molecular epidemiology studies of HTLV-I1 infection and allow for subtype stratification in assessing the sensitivity and specificity of HTLV detection formats and HTLV-II disease association. o 1995 Wiley-Liss, tnc.

Research paper thumbnail of Indicadores de salud perinatal: diferencias entre la información registrada por el INE y la de los hospitales donde se atienden los nacimientos

Revista Española de Salud Pública, 2015

Ariadna Ayerza Casas (1,2) and Néstor Herraiz Esteban (3).

Research paper thumbnail of La rata Wistar como modelo animal experimental, para el adiestramiento laparoscópico de residentes con entrenadores pélvicos

Progresos de Obstetricia y Ginecología, 2011

Entrenador pélvico ginecológico; Rata Wistar; Ejercicios de entrenamiento; Endoscopia ginecológic... more Entrenador pélvico ginecológico; Rata Wistar; Ejercicios de entrenamiento; Endoscopia ginecológica Resumen Presentamos nuevos ejercicios de entrenamiento endoscópico ginecológico en modelo animal (rata Wistar) para formación de los residentes en esta disciplina quirúrgica. Las intervenciones y los ejercicios de adiestramiento se efectuarán en una rata Wistar viva y anestesiada, introducida en un entrenador pélvico endoscópico ginecológico. A través del mismo se realizarán en la rata las prácticas de disección, coagulación, corte, movilización de estructuras de cavidad abdominal, histerectomía subtotal con anexectomía bilateral, extracción de pieza en bolsa y anudado para cerrar la herida abdominal. Con 12 h de trabajo un residente de obstetricia y ginecología en su tercer año conseguiría el dominio de los movimientos necesarios para realizarlos en humanos.

Research paper thumbnail of Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR

Research in Veterinary Science, 2013

Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene... more Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele *1501 has been associated with high proviral load in peripheral blood while allele *0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB3*0902) or susceptibility (BoLA DRB3*1501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR® Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle.

Research paper thumbnail of A rapid and sensitive method of identification of HTLV-II subtypes

Journal of Medical Virology, 1995

There are 2 subtypes of human T-cell lymphoma/ leukemia virus type II (HTLV-11), A and B. HTLV-II... more There are 2 subtypes of human T-cell lymphoma/ leukemia virus type II (HTLV-11), A and B. HTLV-II is increasingly associated with rare forms of lymphocytic neoplasia and a neurodegenerative disorder, characterized by hyperspasticity and ataxia. We have used PCR to amplify, clone and sequence 140 bp of the pol gene from many isolates of HTLV-IIA and HTLV-IIB from around the world. Analysis of these and other published sequences established that all HTLV-IIA sequences contained a unique Hinf I site and all HTLV-IIB sequences a unique Mse I site. A rapid and specific oligomer restriction (OR) assay was developed utilizing the primer pair SKI 10/SK111 and subsequent digestion with these enzymes. Concordance between sequenced and OR-based subtyping of DNA amplified by PCR was absolute among 22 HTLV-II isolates tested. Further OR or sequence analyses on an additional 30 other isolates indicated that the majority of North American non-indian HTLV-I1 isolates were subtype A, while all Paleo-Amerindian samples, including those from the Seminole of Florida; the Guaymi from Panama; and the Toba, Chorote, Wichi, and Chulupe of Argentina, belonged to subtype B. The SKIIO/SKI 11 PCR-OR format should facilitate molecular epidemiology studies of HTLV-I1 infection and allow for subtype stratification in assessing the sensitivity and specificity of HTLV detection formats and HTLV-II disease association. o 1995 Wiley-Liss, tnc.

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