N. Hassanin - Academia.edu (original) (raw)

Papers by N. Hassanin

Veterinary and human toxicology, 1994

A total of 103 Aspergillus flavus isolates from 150 agricultural commodities and animal feed samp... more A total of 103 Aspergillus flavus isolates from 150 agricultural commodities and animal feed samples were tested for aflatoxin production by growing on a semisynthetic liquid medium for 1 w followed by chromatography and spectrophotometric quantification for aflatoxin. All isolates produced 1 or more aflatoxins; 41 isolates (40%) produced all 4 aflatoxins (B1, B2, G1 and G2). Aflatoxin G1 was produced by 81 (80%) of the isolates.

NRIAG Journal of Astronomy and Geophysics, 2014

Twilight observations were carried out in the period between 1984 and 1987 in different seasons a... more Twilight observations were carried out in the period between 1984 and 1987 in different seasons at different sites of Egypt (Baharia, Matrouh, Kottamia and Aswan) through a cooperation project between Dar El-Iftaa' and the Egyptian Academy of Scientific Research and Technology. Naked eye observations of the first light of the dawn were done in parallel to the photoelectric measurements of the twilight phenomena. The depression of the sun below the horizon corresponding to the first light was calculated from the time of observations. Our estimates show that the normal eye can just discriminate the dawn (the first white light thread) at a depression of 14.7°with a maximum value of 15.08°and a minimum value of 12.01°. This result agrees with result obtained by our previous photoelectric measurements.

NRIAG Journal of Astronomy and Geophysics, 2013

Twilight observations were carried out in the period «1983-1985» in different seasons at differen... more Twilight observations were carried out in the period «1983-1985» in different seasons at different sites mainly to deduce the beginning and the end of twilight. One of these sites was Matrouh. These observations were gathered using a refractor (an alt-azimuth scanner) of 10 cm diameter associated with a photoelectric tube of the type (u fi Y À 51) using 3-filters namely; the blue, visual and red filters. The photoelectric system was calibrated with the international photoelectric color system. The phenomena were followed in steps each 10°in both altitude and azimuth. The depression of the sun below the horizon was calculated from the local time of each scan. The results indicated (at a = 5°and A = 10°) for the latitude of Matrouh that the end and the beginning of the twilight occurred when the depression of the sun (D o) was between 18°and 20°, while the dawn times were found to be between 14°and 16°. The brightness of the twilight phenomena was followed, however, to different altitudes and azimuths [0°6 a°6 60°and 0°6 A°6 60°].

African Journal of Biotechnology, 2012

Biodiesel production from microalgae depends on the algal biomass and lipid content. Both biomass... more Biodiesel production from microalgae depends on the algal biomass and lipid content. Both biomass production and lipid accumulation are limited by several factors in which nutrients play a key role. We investigated the influences of micronutrients on biomass, and lipid content of Micractinium pusillum GU732425 cultivated in bold basal media (BBM). The average dry biomass of microalgal strain in control medium reached 0.34 ± 0.01 g /L, while doubling (2X) the levels of Mn and Cu concentration increased the dry biomass to 0.38 ± 0.01 and 0.37 ± 0.02 g /L, respectively. M. pusillum cultivated in control medium had a biomass of 0.82 ± 0.05 g/L and a lipid productivity of 0.33 ± 0.02 g/L after 17 day cultivation. The alga cultivated in BBM with 4X Mn or 4X Cu produced more biomass (1.25 ± 0.01 or 1.28 ± 0.04 g dw/L) and lipid productivity (0.45±0.04 or 0.47±0.05 g/L), respectively. M. pusillum cultivated in different growth media had fatty acid compositions mainly comprising linoleic (49-54%), palmitic (24-29%), linolenic (16-22%), and oleic acids (2-5%). These results can be used to maximize the production of microalgal biomass and lipids in optimally designed photobioreactors.

A. S. HAGGAG, 2022

Objective: Multiple variables affect the probability of development of cancer. The present study ... more Objective: Multiple variables affect the probability of development of cancer. The present study aimed to screen Egyptian females for early prognostic cancer markers such as carcinoembryonic antigen (CEA), the soluble form of transmembrane mucin protein (CA15-3), MUC1 and important sex hormones (Progesteron, Oestrogen, and Prolactin) and three germline BRCA1/2 founder mutations. Patients and Methods: Forty-five DNA samples were screened for 185delAG and 5382insC in the BRCA1 and 6174delT in the BRCA2 genes using polymerase chain reaction (PCR)-directed mutagenesis. Each sample of the 185delAG and the 6174delT mutations was confirmed using Restriction Fragment Length Polymorphism (RFLP) analysis. Nine suspected PCR products of 185delAG and the forty-five amplicons of 6174delT mutations were further confirmed using Sanger sequencing. Sex hormones (Progesteron, Oestrogen, and Prolactin) and cancer antigens (CA 15-3 and CEA) concentrations were quantitatively determined in serum samples using ELISA. Results: We found significant associations only for oestrogen (p-value= 0.036), while non-significant (p-value= 0.123) hyperprolactinemia with cancer history. But none of the individuals carried the BRCA1/2 studied mutations while new variants were detected; (delA) in position 93865, deletion (delA) or substitution of A by G (A/G) in position 93858 and (insA) in position 93844 with frequency of 50%, 50%, 25% and 25%, respectively, in subjects with cancer history. Conclusions: The serum level of oestrogen could be a useful non-invasive cancer marker while significant association of hyperprolactinemia and the new BRCA1/2 variants with cancer needs extra study.

Veterinary and human toxicology, 1994

A total of 103 Aspergillus flavus isolates from 150 agricultural commodities and animal feed samp... more A total of 103 Aspergillus flavus isolates from 150 agricultural commodities and animal feed samples were tested for aflatoxin production by growing on a semisynthetic liquid medium for 1 w followed by chromatography and spectrophotometric quantification for aflatoxin. All isolates produced 1 or more aflatoxins; 41 isolates (40%) produced all 4 aflatoxins (B1, B2, G1 and G2). Aflatoxin G1 was produced by 81 (80%) of the isolates.

NRIAG Journal of Astronomy and Geophysics, 2014

Twilight observations were carried out in the period between 1984 and 1987 in different seasons a... more Twilight observations were carried out in the period between 1984 and 1987 in different seasons at different sites of Egypt (Baharia, Matrouh, Kottamia and Aswan) through a cooperation project between Dar El-Iftaa' and the Egyptian Academy of Scientific Research and Technology. Naked eye observations of the first light of the dawn were done in parallel to the photoelectric measurements of the twilight phenomena. The depression of the sun below the horizon corresponding to the first light was calculated from the time of observations. Our estimates show that the normal eye can just discriminate the dawn (the first white light thread) at a depression of 14.7°with a maximum value of 15.08°and a minimum value of 12.01°. This result agrees with result obtained by our previous photoelectric measurements.

NRIAG Journal of Astronomy and Geophysics, 2013

Twilight observations were carried out in the period «1983-1985» in different seasons at differen... more Twilight observations were carried out in the period «1983-1985» in different seasons at different sites mainly to deduce the beginning and the end of twilight. One of these sites was Matrouh. These observations were gathered using a refractor (an alt-azimuth scanner) of 10 cm diameter associated with a photoelectric tube of the type (u fi Y À 51) using 3-filters namely; the blue, visual and red filters. The photoelectric system was calibrated with the international photoelectric color system. The phenomena were followed in steps each 10°in both altitude and azimuth. The depression of the sun below the horizon was calculated from the local time of each scan. The results indicated (at a = 5°and A = 10°) for the latitude of Matrouh that the end and the beginning of the twilight occurred when the depression of the sun (D o) was between 18°and 20°, while the dawn times were found to be between 14°and 16°. The brightness of the twilight phenomena was followed, however, to different altitudes and azimuths [0°6 a°6 60°and 0°6 A°6 60°].

African Journal of Biotechnology, 2012

Biodiesel production from microalgae depends on the algal biomass and lipid content. Both biomass... more Biodiesel production from microalgae depends on the algal biomass and lipid content. Both biomass production and lipid accumulation are limited by several factors in which nutrients play a key role. We investigated the influences of micronutrients on biomass, and lipid content of Micractinium pusillum GU732425 cultivated in bold basal media (BBM). The average dry biomass of microalgal strain in control medium reached 0.34 ± 0.01 g /L, while doubling (2X) the levels of Mn and Cu concentration increased the dry biomass to 0.38 ± 0.01 and 0.37 ± 0.02 g /L, respectively. M. pusillum cultivated in control medium had a biomass of 0.82 ± 0.05 g/L and a lipid productivity of 0.33 ± 0.02 g/L after 17 day cultivation. The alga cultivated in BBM with 4X Mn or 4X Cu produced more biomass (1.25 ± 0.01 or 1.28 ± 0.04 g dw/L) and lipid productivity (0.45±0.04 or 0.47±0.05 g/L), respectively. M. pusillum cultivated in different growth media had fatty acid compositions mainly comprising linoleic (49-54%), palmitic (24-29%), linolenic (16-22%), and oleic acids (2-5%). These results can be used to maximize the production of microalgal biomass and lipids in optimally designed photobioreactors.

A. S. HAGGAG, 2022

Objective: Multiple variables affect the probability of development of cancer. The present study ... more Objective: Multiple variables affect the probability of development of cancer. The present study aimed to screen Egyptian females for early prognostic cancer markers such as carcinoembryonic antigen (CEA), the soluble form of transmembrane mucin protein (CA15-3), MUC1 and important sex hormones (Progesteron, Oestrogen, and Prolactin) and three germline BRCA1/2 founder mutations. Patients and Methods: Forty-five DNA samples were screened for 185delAG and 5382insC in the BRCA1 and 6174delT in the BRCA2 genes using polymerase chain reaction (PCR)-directed mutagenesis. Each sample of the 185delAG and the 6174delT mutations was confirmed using Restriction Fragment Length Polymorphism (RFLP) analysis. Nine suspected PCR products of 185delAG and the forty-five amplicons of 6174delT mutations were further confirmed using Sanger sequencing. Sex hormones (Progesteron, Oestrogen, and Prolactin) and cancer antigens (CA 15-3 and CEA) concentrations were quantitatively determined in serum samples using ELISA. Results: We found significant associations only for oestrogen (p-value= 0.036), while non-significant (p-value= 0.123) hyperprolactinemia with cancer history. But none of the individuals carried the BRCA1/2 studied mutations while new variants were detected; (delA) in position 93865, deletion (delA) or substitution of A by G (A/G) in position 93858 and (insA) in position 93844 with frequency of 50%, 50%, 25% and 25%, respectively, in subjects with cancer history. Conclusions: The serum level of oestrogen could be a useful non-invasive cancer marker while significant association of hyperprolactinemia and the new BRCA1/2 variants with cancer needs extra study.