Natalia Mitina - Academia.edu (original) (raw)

Papers by Natalia Mitina

Biopolymers and Cell, 2021

Antisense-DNA technologies are new strategy for the treatment of prion infections. This strate gy... more Antisense-DNA technologies are new strategy for the treatment of prion infections. This strate gy requires prolonged administrations of the drugs, which are likely to alter cell redox processes. Aim. The evaluation of cell survival and intensity of oxidative processes in vitro under the influence of antisense-oligodeoxynucleotides (asODNs) as cell prion inhibitors (PrP C) complexed with cationic polyelectrolyte. Methods. Free diffusion in agarose gel, study of cytotoxic action on model cells (bull semen), polarography and potentiometric measurement of oxygen uptake, statistical analysis. Results. Poly(dimethylaminoethyl methacrylate)[-]based surfactants form complexes with asODNs. Polyethylene glycol containing surfactants increase oxygen uptake by cells: by 18 % (VI), by 37 % (IV) and 2.6-fold for V. An addition of the IV-asODNs complex into [the] cell medium did not affect the oxygen absorption; however, it increased reduction processes. Interpolyelectrolyte complex V-asODNs increased the cell respiration by 1.95 times. VI separately increased the cell absorption of oxygen by 18 % and in the complex with asODNs-by 36 %. VI possessed the lowest cytotoxicity. Conclusions. New cationic polyelectrolytes form complexes with asODNs. VI causes the smallest effect on the RedOx processes of model cells and possesses the lowest cytotoxic effect. K e y w o r d s: antisense oligodeoxynucleotides, polyelectrolyte complexes, cytotoxicity, redox processes, prion.

The development of targeted drug delivery using conjugated nanoparticles brings more drug molecul... more The development of targeted drug delivery using conjugated nanoparticles brings more drug molecules to diseased sites, at the same time reducing the negative side effects of systemic drug exposure. In the present study, the binding capability of the newly developed biocompatible PEG-containing polymeric nanocarrier (PNC) was demonstrated. The uptake and cytotoxicity of nanocarrierimmobilized anticancer drugs were enhanced compared to the free drugs. Approximately 10 times lower doses of the PNC complexes achieved similar effects as the free form of the drug on cell cycle arrest, DNA damage, and apoptotic cell death (caspase 7 and PARP cleavage). We investigated anticancer effects of the compounds ID3882, ID3288 and ID3833, the drugs Doxorubicin (Dox) and Temozolomide (TMZ), and PNC complexes containing the compounds ID3882, ID3288 and ID3833 and Dox. PNC complexes demonstrated reduced general toxicity, and enhanced anticancer effects in drugsensitive and drug-resistant tumor cells, ...

2017 IEEE 7th International Conference Nanomaterials: Application & Properties (NAP), 2017

Nanofiber based polymer-inorganic composites filled with superparamagnetic nanoparticles were syn... more Nanofiber based polymer-inorganic composites filled with superparamagnetic nanoparticles were synthesized via electrospinning technique from the suspension of γ-Fe<inf>2</inf>O<inf>3</inf> nanoparticles in dimethylformamide solution of poly(vinylpyrrolidone) (PVP). On the basis of the magnetometric analysis, it was shown that γ-Fe<inf>2</inf>O<inf>3</inf> nanoparticles were in a superparamagnetic state in the nonmagnetic PVP matrix and its content was 10% weight. TEM, EDX analysis and impedance spectroscopy were conducted. It was found that the applied magnetic field (MF) of 2.75 kOe caused a negative giant magnetoresistive effect (GMRE) in the infra-frequency range. For both under normal conditions, and in MF nanofiber (NFM) composite, filled with functionalized maghemite γ-Fe<inf>2</inf>O<inf>3</inf> nanoparticles, NFM/γ-Fe<inf>2</inf>O<inf>3</inf>, the phenomenon of “negative” capacity was detected In addition, the polarization properties of the synthesized material were analyzed. It was proved that applied MF during the measurements increased the dielectric constant ∊ of the material and the magneto-capacitive effect reached 4000% in the infra-frequency range at room temperature. Spin electromotive force (EMF) and 〈spin-battery〉 effect were detected. The possibility of using the synthesized material as high-quality magneto varicaps (in the frequency range of 50÷106 Hz) and quantum batteries (in the frequency range 10−3÷210−2 Hz) was proved.

The development of novel nanoparticlesand nanocomposites with polymeric shellwhich provides their... more The development of novel nanoparticlesand nanocomposites with polymeric shellwhich provides their colloidal stability withbiocompatibility, chemical targeted functionality and reactivity as well as the methods oftheir synthesis are of a significant interest forthe obtaining nanoscale carriers and labels ofbiomedical application [1–3]. A variety of synthetic technologies for the preparation ofnanoparticles of biomedical application withnarrowed size distribution and tailored functionality and reactivity are available [1, 2]. Atthe same time nanoparticle targeted irreversible surface functionalization is one of themain problems of the particle synthesis andbiomedical application. As it is referred in [4]«The ability to assemble nanostructuresrequires precise control of the particle’s surface chemistry, where molecules can be coatedonto the surface to direct the assemblyprocess. Strategies have been developed toreadily permit the modification of a nanoparticle’s surface chemistry. In p...

2021 IEEE 11th International Conference Nanomaterials: Applications & Properties (NAP), 2021

This work develops a new approach to the synthesis of nanocomposites and their study as effective... more This work develops a new approach to the synthesis of nanocomposites and their study as effective materials for lithium current sources. This paper proposes a unique method that allows obtaining stable composite materials in which nanosized MoS2 particles are fixed in the pores of SiO2. To obtain nanocomposites with preset morphological characteristics, the method of hydrothermal synthesis is used, followed by modification of the surface of nanoparticles with functional polymers via polymerization initiated from the particle surface. TEM, impedance spectroscopy, and computer simulation of an equivalent electrical schema are used to characterize such materials before and after grafting a functional polymer shell onto their surface. The prospects of using synthesized composites as cathode materials of lithium current sources, as well as the possibility of controlling the structure and size of the inorganic nucleus and a functional polymer shell, are proved. The kinetics and thermodynamics of Li+-intercalation current generation are studied.

Journal of Nanoscience and Nanotechnology, 2019

A novel flexible electrospun nanofiber/-Fe 2 O 3 composite has been obtained from suspension of-F... more A novel flexible electrospun nanofiber/-Fe 2 O 3 composite has been obtained from suspension of-Fe 2 O 3 nanoparticles in polyvinylpyrolidone solution in dimethylformamide. The impedance spectroscopy of the synthesized nanofiber/-Fe 2 O 3 composite was carried out. Negative magnetoresistance and giant magnetocapacitance effects, as well as phenomenon of a "negative capacitance" at room temperature were observed in magnetic field (2.75 Oe) in infra-low frequency range. The polarization properties and volt-ampere characteristics of the nanocomposite in the applied magnetic field indicate the increase in the dielectric permittivity and the emergence of spin electromotive force, which enables us to accumulate of electric energy at quantum level. A quantum-mechanical model, which explained the non-monotonous behaviour of the volt-ampere characteristic of the novel nanofiber based composite, has been suggested.

Plant Cell, Tissue and Organ Culture (PCTOC), 2017

and plasmid DNA, as well as the DNA protection by the PDMAEM polymers against nuclease degradatio... more and plasmid DNA, as well as the DNA protection by the PDMAEM polymers against nuclease degradation were confirmed by electrophoresis in agarose gel. In addition, model organisms Allium cepa and Nicotiana tabacum L. were studied to evaluate cytotoxic effect of the PDMAEM carriers. The created PDMAEM-based carriers were effective in delivery of plasmid DNA into moss and tabacco protoplasts (obtaining stable transformants of Ceratodon purpureus moss, as well as in transient expression of the reporter yfp gene product in N. tabacum protoplasts). Thus, novel PDMAEM-based polymers were shown to be promising carriers for delivery of DNA into plant cells, and carriers possess high potential for further applications in this field.

Encyclopedia of Surface and Colloid Science, Third Edition, 2015

Cytology and Genetics, 2014

There is much progress in application of genetic engineering for improving the biological proper ... more There is much progress in application of genetic engineering for improving the biological proper ties of different organisms. Viral and nonviral carriers are used for delivery of genetic material into target cells. Polymeric materials of natural and synthetic origin are the most promising gene delivery agents. These poly mers demonstrated high efficiency of DNA delivery into animal cells, although they were not very effective in plant cells. Here, the procedure for genetic transformation of Ceratodon purpureus (Hedw.) Brid. moss pro toplasts is described. The method is based on the application of surface active polymeric carriers of the poly DMAEM structure and controlled length and charge. This allows obtaining more transient and stable moss transformants per microgram of plasmid DNA when compared with known protocol based on using polyeth yleneglycol. It is easier, more convenient, and cheaper than the "gene gun" method. Prospects for further improvement of structure and functional characteristics of new polymeric carriers are considered for delivery of genetic material into plant cells.

Macromolecular Symposia, 2001

Journal of Polymer Science Part A: Polymer Chemistry, 2000

Carbon-chain heterofunctional oligoperoxides (HFOs) with ditertiary peroxidic, carboxy, or hydrox... more Carbon-chain heterofunctional oligoperoxides (HFOs) with ditertiary peroxidic, carboxy, or hydroxy groups formed stable coordinated complexes through their interaction with transition-metal salts in organic solutions. The oligomeric metal complexes (OMCs) were separated from the reaction mixture and investigated through elemental analysis, voltammetry, and atom-adsorption, IR, and UV spectroscopy. From these investigations and the calculated values of the mole fraction and formation constants of the stable complexes of the copper cations and HFOs, the probable cooperative mechanism of the complex formation by such oligomers was suggested. The existence of two levels of HFO molecule ordering, namely HFO microstructure and the structure of the hydrogen-bonded complexes formed by HFOs, explained the cooperative mechanism and some characteristics of the OMC formation. The mole fraction and formation constants of the OMCs were interrelated with the disposition of the complexforming groups in the chain of HFOs. From the experimental data, it can be concluded that the complex-forming groups of the HFOs possessed different reactivities both in the complex formation with metal cations and in the reactions involving the resulting OMCs. The shift in the half-wave potential of the OO:OO bond reduction during the transition from the HFO to a mixture of HFO and Cu 2ϩ and to OMC indicated an increase in its reactivity.

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2014

Chemosphere, 2012

Cobalt (Co 2+) is present in many nanoscaled materials created for various applications. The key ... more Cobalt (Co 2+) is present in many nanoscaled materials created for various applications. The key goal of our study was to develop sensitive approaches for assessing the bio-risks associated with using novel Co 2+containing nanoscaled polymeric complex (Co-NC). Freshwater bivalve mollusk Anodonta cygnea (Unionidae) was subjected to 14 d action of the developed Co-NC, as well as of Co 2+ applied in the corresponding concentration (50 lg L À1) or polymeric substance (PS). All experimental groups under study have demonstrated signs of toxic targeting, notably changes in DNA characteristics, oxidative stress (with particularities in each exposed group) and activation of anaerobiosis (Co 2+ and Co-NC). However, the group exposed to Co-NC showed some advantages that can be related to the activation of metallothionein (MT) function (increase in the level of MT-related SH-groups (MT-SH)): low level of oxyradical formation, no increase in protein carbonylation and vitellogenin-like proteins concentration unlike in Co 2+ and PS exposed groups. On the other hand, Co 2+ increased metal (Co, Cu, Zn and Cd) binding to MT (MT-Me) without changes in MT-SH level jointly with activation of oxyradical formation and apoptosis and decreasing of lysosomal membrane stability. PS per se initiated unbalanced changes in activities of the biotransformation enzymes ethoxyresorufin-O-deethylase and glutathione-S-transferase. Thus, Co 2+ complexing with the developed PS prevented bio-toxic effects of free Co 2+ ions and PS per se, at least in the studied hydrobiont. The MT-SH was the main distinguishing index of Co-NC group selected by classification and regression tree analysis.

Principal experimental approaches based on tailored synthesis of oligoperoxide surfaceactive subs... more Principal experimental approaches based on tailored synthesis of oligoperoxide surfaceactive substances and their application for obtaining polymeric and hybrid nanoscale carriers possessing targeted functionality and biocompatibility are presented. Molecular ...

Cancer Nanotechnology, 2018

The synthesis of block-copolymers of controlled chain length and content of end peroxide groups v... more The synthesis of block-copolymers of controlled chain length and content of end peroxide groups via polymerization of dimethylaminoethyl methacrylate initiated by Ce 4+-PEG system using a functional peroxidecontaining chain transfer agent was developed. Rheological and colloidal-chemical characteristics of novel polyampholitic surfactants were studied.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology

Despite numerous studies are suggesting a dramatic decline of amphibians, the biochemical mechani... more Despite numerous studies are suggesting a dramatic decline of amphibians, the biochemical mechanisms of adaptation in these animals to polluted environment are poorly studied. The aim of this study was to elucidate the ability to release cobalt (Co) and zinc (Zn) from their nanoscale complexes (NC) derived from the polymeric substance of N-vinylpyrrolidone (PS) in the liver of amphibian (Rana ridibunda). Frog males were subjected to 14 day exposure to waterborne Co(2+) (50 μg/L), Zn(2+) (100 μg/L), as well as corresponding concentrations of Co-NC, Zn-NC or PS. Main attention was paid to MT's interrelations with indices of stress and toxicity. Only Co(2+) and Zn(2+) caused elevation of the correspondent metal in MTs. Co(2+) caused down-regulation of cathepsin D activity, while Zn(2+), Zn-NC and the PS up-regulated this activity. Zn(2+) provoked 1.6 times increase of metal-bounded form of the MT (MT-Me), while all other exposures caused the elevation of the ratio of MT total prote...

Macromolecular …, 2001

Controlled surface activity and reactivity of new carbon-chain functional surface-active oligoper... more Controlled surface activity and reactivity of new carbon-chain functional surface-active oligoperoxides (FSAP) and derived metal complexes (OMC) with side and end di-tertiary and tert-alkyl (aryl) peroxidic, respectively, and other active functional groups cause the possibility of their ...