Nisha Patro - Academia.edu (original) (raw)

Papers by Nisha Patro

Research paper thumbnail of Cumulative Exposure to Multiple Early Life Stressors Act as Risk Factors for Neurodevelopmental Abnormalities in the F1 Wistar Rats

Cumulative exposure to multiple early life stressors are expected to affect behavioral developmen... more Cumulative exposure to multiple early life stressors are expected to affect behavioral development causing increased susceptibility towards neuropsychiatric disorders. The present study was designed to mimic such conditions in rat model to study behavioral impairments during adolescence and adulthood. Female Wistar rats (n=32; 140-150 gm) were switched to low protein (LP; 8% protein) or Control (20% protein) diet, 15 days prior to conception and then diet regime was maintained throughout the experimental period. Pups born to Control and LP dams were intraperitoneally injected with deltamethrin (DLT-pyrethroid insecticide; 0.7mg/kg body weight; PND 1 to 7) or lipopolysaccharide (LPS-bacterial endotoxin; 0.3mg/kg body weight; PND 3 and 5) or DLT+LPS, on designated days forming 8 experimental groups (Control, LP, Control+LPS, LP+LPS, Control+DLT, LP+DLT, Control+DLT+LPS and LP+DLT+LPS). Neurobehavioral assessments were performed in F1 rats (1, 3, 6 months) by Open field, Elevated plus maze, Light and Dark box, and Rotarod tests. LP rats were found to be more susceptible to either singular or cumulative exposure as compared to their age matched control counterparts, showing significantly severe behavioural abnormalities, as hyperactivity, attention deficits, impulsiveness and low anxiety, the hallmark symptoms of neuropsychiatric disorders like schizophrenia and ADHD, suggesting thereby that early life multi-hit exposure may predispose individuals to developmental disorders.

Research paper thumbnail of Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat

Journal of Chemical Neuroanatomy, Nov 1, 2022

Research paper thumbnail of Astrogliosis and associated CSPG upregulation adversely affect dendritogenesis, spinogenesis and synaptic activity in the cerebellum of a double-hit rat model of protein malnutrition (PMN) and lipopolysaccharide (LPS) induced bacterial infection

Journal of Chemical Neuroanatomy, Sep 1, 2023

Research paper thumbnail of Study on the cognitive changes in STZ-induced diabetes with special reference to glial changes and immune activation in rat hippocampus

Research paper thumbnail of Multiple Early Life Stressors as Risk Factors for Neurodevelopmental Abnormalities in the F1 Wistar Rats

Brain Sciences

Cumulative exposure to multiple early life stressors is expected to affect behavioral development... more Cumulative exposure to multiple early life stressors is expected to affect behavioral development, causing increased susceptibility to neuropsychiatric disorders. The present study was designed to mimic such conditions in a rat model to study behavioral impairments during adolescence and adulthood. Female Wistar rats (n = 32; 140–150 gm) were switched to a low protein (LP; 8% protein) or control (20% protein) diet 15 days prior to conception, and then the diet regime was maintained throughout the experimental period. Pups born to control and LP dams were intraperitoneally injected with deltamethrin (DLT—pyrethroid insecticide; 0.7 mg/kg body weight; PND 1 to 7), lipopolysaccharide (LPS—bacterial endotoxin; 0.3 mg/kg body weight; PND 3 and 5), or DLT+LPS, on designated days forming eight experimental groups (Control, LP, Control+LPS, LP+LPS, Control+DLT, LP+DLT, Control+DLT+LPS and LP+DLT+LPS). Neurobehavioral assessments were performed in F1 rats (1, 3, 6 months) by open field, elev...

Research paper thumbnail of The Glial Perspective of Energy Homeostasis, Neuroinflammation, and Neuro-nutraceuticals

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of A Sequential Study of Age-Related Lipofuscin Accumulation in Hippocampus and Striate Cortex of Rats

Annals of Neurosciences, 2018

Background: The age-pigment, lipofuscin that accumulates in cells intrinsically and progressively... more Background: The age-pigment, lipofuscin that accumulates in cells intrinsically and progressively with age is considered as the hallmark of aging. This accumulation is more prominent in post-mitotic cells like neurons and also appears in glia. Purpose: The aim of the present study was to assess the age-associated occurrence and distribution pattern of lipofuscin both in neurons and microglia in various regions of hippocampus and striate cortex. Methods: The accumulation pattern of lipofuscin in hippocampus and striate cortex was observed in the female Wistar rats of 6 age groups, that is, 3, 6, 12, 18, 24, and 30 months using the autofluorescent property of lipofuscin and its specific localization in neurons and microglia by dual immunolabeling with NeuN and Iba-1 antibodies respectively. Cytoarchitectural and the morphological age-related changes were observed with cresyl violet staining. Results: Lipofuscin pigments accumulate progressively through the normal aging process in hippocampus and striate cortex. However, in hippocampus, lipofuscin accumulates in a region-specific manner with the highest accumulation observed in cornu ammonis (CA) 1 and 3 subregions. Furthermore, the lipofuscin accumulation was also observed in microglia in the senile brains both in the hippocampus and striate cortex. Conclusion: The progressive deposition of lipofuscin could result in cellular dysfunction. This encouraged us to forward the idea that microglia not only participate in the removal of the pigment from the neurons but also accumulate these waste products in itself. Such senescent microglia may contribute to age-related neurodegeneration as they lose the neuroprotective potential due to oxidative stress and thus may be unable to effectively phagocytose the age pigment.

Research paper thumbnail of Perineuronal nets: Cruise from a honeycomb to the safety nets

Research paper thumbnail of <i>Spirulina platensis</i> protects neurons via suppression of glial activation and peripheral sensitization leading to restoration of motor function in collagen-induced arthritic rats

739-748Spirulina platensis treatment (400 mg kg-1 for 25 days) effectively suppressed peripheral ... more 739-748Spirulina platensis treatment (400 mg kg-1 for 25 days) effectively suppressed peripheral sensitization via modulation of glial activation and improved motor coordination and restoration of functional motor activity in collagen-induced arthritic rats. Spirulina treatment also resulted in an appreciable reduction of the NF200 accumulation in the spinal cord neurons of arthritic rats. This is indicative of neuroprotective action of S. platensis against glutamate excitotoxicity-induced central sensitization produced by the peripheral joint inflammation in the collagen-induced arthritis. The results suggest that effects of S. platensis may be due to its counter regulation of spinal glial activation and could be a potential strategy for the treatment of arthritis

Research paper thumbnail of Generation and Maturation of Macroglia in the Central Nervous System

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Intra-generational Protein Malnutrition

Research paper thumbnail of Effect of postnatal viral infection on the sensitivity of neurotransmitter receptors and expression of neuronal immediate early genes in developing rat brain

F1000Research, 2013

ABSTRACT Background / Purpose: Early life infection is known to activate immune responses in the ... more ABSTRACT Background / Purpose: Early life infection is known to activate immune responses in the brain that may result in the development of various psychiatric and neurodegenerative disorders in later life. We aim to investigate the molecular underpinnings of early life infection on synaptic plasticity and neurotransmitter receptor sensitivity. Main conclusion: Overexpression of NARP, Homer1a and Egr1 have implications in impairment in maintenance of LTP, poor spatial working memory and imbalance of protein degradation and synthesis, respectively.Decreased (p less than 0.05) sensitivity of striatal DA-D2R are underlying factors for developing addiction-promoting behaviours.

Research paper thumbnail of Perinatal exposure to synergistic multiple stressors lead to cellular and behavioral deficits mimicking Schizophrenia like pathology

Biology Open, 2022

Protein malnourishment and immune stress are potent perinatal stressors, encountered by children ... more Protein malnourishment and immune stress are potent perinatal stressors, encountered by children born under poor socioeconomic condition. Thus, it is necessary to investigate how such stressors synergistically contribute towards developing neurological disorders in affected individuals. Pups from Wistar females, maintained on normal (high protein:HP:20%) and low protein (LP:8%) diets were used. Single and combined exposures of Poly I:C (viral mimetic:5mg/kg body wt.) and LPS (bacterial endotoxin:0.3 mg/kg body wt.) were injected to both HP and LP pups at postnatal day (PND) 3 and 9 respectively, creating 8 groups: HP (control), HP+Poly I:C, HP+LPS, HP+Poly I:C+LPS, LP, LP+Poly I:C, LP+LPS, LP+Poly I:C+LPS (multi-hit). The effects of stressors on hippocampal cytoarchitecture and behavioral abilities were studied at PND 180. LP animals were found vulnerable to immune stressors then HP animals and symptoms like neuronal damage, spine loss, downregulation of Egr 1 and Arc proteins, glio...

Research paper thumbnail of Origin and Development of Microglia

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Oligodendrocyte: Structure, Function and Pathology

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Reviewed by

doi: 10.3389/fncel.2014.00355 Experimentally induced diabetes causes glial activation, glutamate ... more doi: 10.3389/fncel.2014.00355 Experimentally induced diabetes causes glial activation, glutamate toxicity and cellular damage leading to changes in motor function

Research paper thumbnail of Amelioration of neurobehavioral and cognitive abilities of F1 progeny following dietary supplementation with Spirulina to protein malnourished mothers

Brain, Behavior, and Immunity, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Lipofuscin accumulation in ageing myocardium & its removal by meclophenoxate

The Indian journal of medical research, 1992

A study was undertaken on the age-associated histochemical changes in the ventricular myocardium ... more A study was undertaken on the age-associated histochemical changes in the ventricular myocardium and the influence of meclophenoxate hydrochloride (MPH) on the age pigment lipofuscin. Sixty Wistar albino rats in three age-groups (3, 15 and 30 months old) were treated with meclophenoxate hydrochloride (100 mg/kg body wt/day, ip) for a period of 2-8 wk. Five animals each from the three age-groups served as controls. Various histochemical and micromorphometric studies were carried out on the myocardial tissue. A linear increase in the myocardial volume occupied by the pigment was observed with advancing age. As a result of meclophenoxate treatment, a gradual decrease in the myocardial volume occupied by the pigment was noted. After 4-6 wk treatment, the pigment bodies were found lodged into the capillary endothelium and the lumen, facilitating the removal of the pigment via blood stream. Histochemical and micromorphometric analyses of ventricular myocardium of albino rats have shown th...

Research paper thumbnail of Microglia Aging

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Developmental Changes in Oligodendrocyte Genesis, Myelination, and Associated Behavioral Dysfunction in a Rat Model of Intra-generational Protein Malnutrition

Molecular Neurobiology, 2018

Impairments in oligodendrocyte development and resultant myelination deficits appear as a common ... more Impairments in oligodendrocyte development and resultant myelination deficits appear as a common denominator to all neurological diseases. An optimal in utero environment is obligatory for normal fetal brain development and later life brain functioning. Late embryonic and early postnatal brains from F1 rat born to protein malnourished mothers were studied through a combination of immunocytochemical and quantitative PCR assay for analyzing the relative expression of platelet-derived growth factor receptor-α (PDGFRα), myelin-associated glycoprotein (MAG), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG) to determine oligodendrocyte genesis, differentiation, maturation, and myelination. Myelin integrity and corpus callosum caliber was assessed by Luxol fast blue (LFB) staining, whereas grip strength test and open field activity monitoring for behavioral evaluation in F1 rats. We demonstrate that intra-generational protein deprivation results in drastically low PDGFRα+ oligodendrocyte precursor (OPC) population and significantly reduced expression of myelin protein genes resulting in poor pre-myelinating and mature myelinating oligodendrocyte number, hypo-myelination, and misaligned myelinated fibers. LFB staining and MOG immunolabeling precisely revealed long-term changes in corpus callosum (CC) caliber and demyelination lesions in LP brain supporting the behavioral and cognitive changes at early adolescence and adulthood following maternal protein malnutrition (PMN). Thus, intra-generational PMN negatively affects the oligodendrocyte development and maturation resulting in myelination impairments and associated with behavioral deficits typically mimicking clinical hallmarks of neuropsychiatric disorders. Our results further strengthen and augment the hypothesis “Impaired gliogenesis is a big hit for neuropsychiatric phenotype.”

Research paper thumbnail of Cumulative Exposure to Multiple Early Life Stressors Act as Risk Factors for Neurodevelopmental Abnormalities in the F1 Wistar Rats

Cumulative exposure to multiple early life stressors are expected to affect behavioral developmen... more Cumulative exposure to multiple early life stressors are expected to affect behavioral development causing increased susceptibility towards neuropsychiatric disorders. The present study was designed to mimic such conditions in rat model to study behavioral impairments during adolescence and adulthood. Female Wistar rats (n=32; 140-150 gm) were switched to low protein (LP; 8% protein) or Control (20% protein) diet, 15 days prior to conception and then diet regime was maintained throughout the experimental period. Pups born to Control and LP dams were intraperitoneally injected with deltamethrin (DLT-pyrethroid insecticide; 0.7mg/kg body weight; PND 1 to 7) or lipopolysaccharide (LPS-bacterial endotoxin; 0.3mg/kg body weight; PND 3 and 5) or DLT+LPS, on designated days forming 8 experimental groups (Control, LP, Control+LPS, LP+LPS, Control+DLT, LP+DLT, Control+DLT+LPS and LP+DLT+LPS). Neurobehavioral assessments were performed in F1 rats (1, 3, 6 months) by Open field, Elevated plus maze, Light and Dark box, and Rotarod tests. LP rats were found to be more susceptible to either singular or cumulative exposure as compared to their age matched control counterparts, showing significantly severe behavioural abnormalities, as hyperactivity, attention deficits, impulsiveness and low anxiety, the hallmark symptoms of neuropsychiatric disorders like schizophrenia and ADHD, suggesting thereby that early life multi-hit exposure may predispose individuals to developmental disorders.

Research paper thumbnail of Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat

Journal of Chemical Neuroanatomy, Nov 1, 2022

Research paper thumbnail of Astrogliosis and associated CSPG upregulation adversely affect dendritogenesis, spinogenesis and synaptic activity in the cerebellum of a double-hit rat model of protein malnutrition (PMN) and lipopolysaccharide (LPS) induced bacterial infection

Journal of Chemical Neuroanatomy, Sep 1, 2023

Research paper thumbnail of Study on the cognitive changes in STZ-induced diabetes with special reference to glial changes and immune activation in rat hippocampus

Research paper thumbnail of Multiple Early Life Stressors as Risk Factors for Neurodevelopmental Abnormalities in the F1 Wistar Rats

Brain Sciences

Cumulative exposure to multiple early life stressors is expected to affect behavioral development... more Cumulative exposure to multiple early life stressors is expected to affect behavioral development, causing increased susceptibility to neuropsychiatric disorders. The present study was designed to mimic such conditions in a rat model to study behavioral impairments during adolescence and adulthood. Female Wistar rats (n = 32; 140–150 gm) were switched to a low protein (LP; 8% protein) or control (20% protein) diet 15 days prior to conception, and then the diet regime was maintained throughout the experimental period. Pups born to control and LP dams were intraperitoneally injected with deltamethrin (DLT—pyrethroid insecticide; 0.7 mg/kg body weight; PND 1 to 7), lipopolysaccharide (LPS—bacterial endotoxin; 0.3 mg/kg body weight; PND 3 and 5), or DLT+LPS, on designated days forming eight experimental groups (Control, LP, Control+LPS, LP+LPS, Control+DLT, LP+DLT, Control+DLT+LPS and LP+DLT+LPS). Neurobehavioral assessments were performed in F1 rats (1, 3, 6 months) by open field, elev...

Research paper thumbnail of The Glial Perspective of Energy Homeostasis, Neuroinflammation, and Neuro-nutraceuticals

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of A Sequential Study of Age-Related Lipofuscin Accumulation in Hippocampus and Striate Cortex of Rats

Annals of Neurosciences, 2018

Background: The age-pigment, lipofuscin that accumulates in cells intrinsically and progressively... more Background: The age-pigment, lipofuscin that accumulates in cells intrinsically and progressively with age is considered as the hallmark of aging. This accumulation is more prominent in post-mitotic cells like neurons and also appears in glia. Purpose: The aim of the present study was to assess the age-associated occurrence and distribution pattern of lipofuscin both in neurons and microglia in various regions of hippocampus and striate cortex. Methods: The accumulation pattern of lipofuscin in hippocampus and striate cortex was observed in the female Wistar rats of 6 age groups, that is, 3, 6, 12, 18, 24, and 30 months using the autofluorescent property of lipofuscin and its specific localization in neurons and microglia by dual immunolabeling with NeuN and Iba-1 antibodies respectively. Cytoarchitectural and the morphological age-related changes were observed with cresyl violet staining. Results: Lipofuscin pigments accumulate progressively through the normal aging process in hippocampus and striate cortex. However, in hippocampus, lipofuscin accumulates in a region-specific manner with the highest accumulation observed in cornu ammonis (CA) 1 and 3 subregions. Furthermore, the lipofuscin accumulation was also observed in microglia in the senile brains both in the hippocampus and striate cortex. Conclusion: The progressive deposition of lipofuscin could result in cellular dysfunction. This encouraged us to forward the idea that microglia not only participate in the removal of the pigment from the neurons but also accumulate these waste products in itself. Such senescent microglia may contribute to age-related neurodegeneration as they lose the neuroprotective potential due to oxidative stress and thus may be unable to effectively phagocytose the age pigment.

Research paper thumbnail of Perineuronal nets: Cruise from a honeycomb to the safety nets

Research paper thumbnail of <i>Spirulina platensis</i> protects neurons via suppression of glial activation and peripheral sensitization leading to restoration of motor function in collagen-induced arthritic rats

739-748Spirulina platensis treatment (400 mg kg-1 for 25 days) effectively suppressed peripheral ... more 739-748Spirulina platensis treatment (400 mg kg-1 for 25 days) effectively suppressed peripheral sensitization via modulation of glial activation and improved motor coordination and restoration of functional motor activity in collagen-induced arthritic rats. Spirulina treatment also resulted in an appreciable reduction of the NF200 accumulation in the spinal cord neurons of arthritic rats. This is indicative of neuroprotective action of S. platensis against glutamate excitotoxicity-induced central sensitization produced by the peripheral joint inflammation in the collagen-induced arthritis. The results suggest that effects of S. platensis may be due to its counter regulation of spinal glial activation and could be a potential strategy for the treatment of arthritis

Research paper thumbnail of Generation and Maturation of Macroglia in the Central Nervous System

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Intra-generational Protein Malnutrition

Research paper thumbnail of Effect of postnatal viral infection on the sensitivity of neurotransmitter receptors and expression of neuronal immediate early genes in developing rat brain

F1000Research, 2013

ABSTRACT Background / Purpose: Early life infection is known to activate immune responses in the ... more ABSTRACT Background / Purpose: Early life infection is known to activate immune responses in the brain that may result in the development of various psychiatric and neurodegenerative disorders in later life. We aim to investigate the molecular underpinnings of early life infection on synaptic plasticity and neurotransmitter receptor sensitivity. Main conclusion: Overexpression of NARP, Homer1a and Egr1 have implications in impairment in maintenance of LTP, poor spatial working memory and imbalance of protein degradation and synthesis, respectively.Decreased (p less than 0.05) sensitivity of striatal DA-D2R are underlying factors for developing addiction-promoting behaviours.

Research paper thumbnail of Perinatal exposure to synergistic multiple stressors lead to cellular and behavioral deficits mimicking Schizophrenia like pathology

Biology Open, 2022

Protein malnourishment and immune stress are potent perinatal stressors, encountered by children ... more Protein malnourishment and immune stress are potent perinatal stressors, encountered by children born under poor socioeconomic condition. Thus, it is necessary to investigate how such stressors synergistically contribute towards developing neurological disorders in affected individuals. Pups from Wistar females, maintained on normal (high protein:HP:20%) and low protein (LP:8%) diets were used. Single and combined exposures of Poly I:C (viral mimetic:5mg/kg body wt.) and LPS (bacterial endotoxin:0.3 mg/kg body wt.) were injected to both HP and LP pups at postnatal day (PND) 3 and 9 respectively, creating 8 groups: HP (control), HP+Poly I:C, HP+LPS, HP+Poly I:C+LPS, LP, LP+Poly I:C, LP+LPS, LP+Poly I:C+LPS (multi-hit). The effects of stressors on hippocampal cytoarchitecture and behavioral abilities were studied at PND 180. LP animals were found vulnerable to immune stressors then HP animals and symptoms like neuronal damage, spine loss, downregulation of Egr 1 and Arc proteins, glio...

Research paper thumbnail of Origin and Development of Microglia

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Oligodendrocyte: Structure, Function and Pathology

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Reviewed by

doi: 10.3389/fncel.2014.00355 Experimentally induced diabetes causes glial activation, glutamate ... more doi: 10.3389/fncel.2014.00355 Experimentally induced diabetes causes glial activation, glutamate toxicity and cellular damage leading to changes in motor function

Research paper thumbnail of Amelioration of neurobehavioral and cognitive abilities of F1 progeny following dietary supplementation with Spirulina to protein malnourished mothers

Brain, Behavior, and Immunity, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Lipofuscin accumulation in ageing myocardium & its removal by meclophenoxate

The Indian journal of medical research, 1992

A study was undertaken on the age-associated histochemical changes in the ventricular myocardium ... more A study was undertaken on the age-associated histochemical changes in the ventricular myocardium and the influence of meclophenoxate hydrochloride (MPH) on the age pigment lipofuscin. Sixty Wistar albino rats in three age-groups (3, 15 and 30 months old) were treated with meclophenoxate hydrochloride (100 mg/kg body wt/day, ip) for a period of 2-8 wk. Five animals each from the three age-groups served as controls. Various histochemical and micromorphometric studies were carried out on the myocardial tissue. A linear increase in the myocardial volume occupied by the pigment was observed with advancing age. As a result of meclophenoxate treatment, a gradual decrease in the myocardial volume occupied by the pigment was noted. After 4-6 wk treatment, the pigment bodies were found lodged into the capillary endothelium and the lumen, facilitating the removal of the pigment via blood stream. Histochemical and micromorphometric analyses of ventricular myocardium of albino rats have shown th...

Research paper thumbnail of Microglia Aging

The Biology of Glial Cells: Recent Advances

Research paper thumbnail of Developmental Changes in Oligodendrocyte Genesis, Myelination, and Associated Behavioral Dysfunction in a Rat Model of Intra-generational Protein Malnutrition

Molecular Neurobiology, 2018

Impairments in oligodendrocyte development and resultant myelination deficits appear as a common ... more Impairments in oligodendrocyte development and resultant myelination deficits appear as a common denominator to all neurological diseases. An optimal in utero environment is obligatory for normal fetal brain development and later life brain functioning. Late embryonic and early postnatal brains from F1 rat born to protein malnourished mothers were studied through a combination of immunocytochemical and quantitative PCR assay for analyzing the relative expression of platelet-derived growth factor receptor-α (PDGFRα), myelin-associated glycoprotein (MAG), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG) to determine oligodendrocyte genesis, differentiation, maturation, and myelination. Myelin integrity and corpus callosum caliber was assessed by Luxol fast blue (LFB) staining, whereas grip strength test and open field activity monitoring for behavioral evaluation in F1 rats. We demonstrate that intra-generational protein deprivation results in drastically low PDGFRα+ oligodendrocyte precursor (OPC) population and significantly reduced expression of myelin protein genes resulting in poor pre-myelinating and mature myelinating oligodendrocyte number, hypo-myelination, and misaligned myelinated fibers. LFB staining and MOG immunolabeling precisely revealed long-term changes in corpus callosum (CC) caliber and demyelination lesions in LP brain supporting the behavioral and cognitive changes at early adolescence and adulthood following maternal protein malnutrition (PMN). Thus, intra-generational PMN negatively affects the oligodendrocyte development and maturation resulting in myelination impairments and associated with behavioral deficits typically mimicking clinical hallmarks of neuropsychiatric disorders. Our results further strengthen and augment the hypothesis “Impaired gliogenesis is a big hit for neuropsychiatric phenotype.”