Naim Saglam - Academia.edu (original) (raw)

Papers by Naim Saglam

Engineering sciences, Jan 25, 2024

KAFESLERDE YETİŞTİRİLEN GÖKKUŞAĞI ALABALIĞINDA (Oncorhynchus mykiss) MYXOSPOREAN (MYXOZOA) TÜRLER... more KAFESLERDE YETİŞTİRİLEN GÖKKUŞAĞI ALABALIĞINDA (Oncorhynchus mykiss) MYXOSPOREAN (MYXOZOA) TÜRLERİNİN ARAŞTIRILMASI ÖZ Bu çalışma Elazığ Keban ve Karakaya Baraj göllerinde kafeslerde yetiştiriciliği yapılan gökkuşağı alabalığında (Oncorhynchus mykiss) Myxosporean parazitlerin enfeksiyon ve histopatolojik durumunu belirlemek amacıyla yapılmıştır. Alabalık örnekleri kapasitesi 250 ton ve üzerinde olan ve farklı bölgeleri temsil eden altı kafes işletmesinden Ocak-Aralık 2014 ve Ocak-Aralık 2016 tarihleri arasında iki yıl süreyle toplanmıştır. Parazitolojik ve histopatolojik incelemesi yapılan 1080 gökkuşağı alabalığının 29'unun (%2.69) solungaç lamellalarında Myxobolus sp. tespit edilmiştir. En yüksek enfeksiyon prevalansı (%10.00) Keban baraj gölünün 6. Bölgesinde yer alan alabalık çiftliklerinde belirlenirken, en düşük enfeksiyon prevalansı (%1.11) Keban baraj gölünün 3. Bölgesinde gözlenmiştir. Keban Baraj gölünde yer alan alabalık kafes işletmelerde Myxobolus sp'nin prevalansı (%4.07), Karakaya Baraj Gölünden (%1.30) daha yüksek tespit edilmiştir. Myxobolus sp kistlerinin alabalığın sekonder solungaç lamellalardaki kapillar damarlara yerleştiği, hipertrofi ve hiperplazi gibi patolojik bozukluklar oluşturduğu belirlenmiştir. Myxosporean parazit türlerinin kafes işletmelerinde moleküler olarak rutin takibinin başlatılması kesin tür teşhisi ve salgınlara dönüşmeden önlemlerin alınması açısından önem taşımaktadır. Anahtar Kelimeler: Gökkuşağı Alabalığı, Protozoa, Myxosporean, Myxobolus, Histopatoloji THE STUDY OF MYXOSPOREAN (MYXOZOA) SPECIES IN RAINBOW TROUT (Oncorhynchus mykiss) FARMED IN CAGES ABSTRACT This study was conducted to determine the infection and histopathological status of Myxosporean parasites in rainbow trout (Oncorhynchus mykiss) cultured in cages in Elazığ Keban and Karakaya reservoirs. Rainbow trout samples were collected from six cage farms with a capacity of 250 tons or more and representing different regions for two years between January-December 2014 and January-December 2016. Myxobolus sp. was detected in the gill lamellae of 29 (2.69%) out of 1080 rainbow trout that were parasitologically and histopathologically examined. The highest infection prevalence (10.00%) was determined in trout farms located in the 6th region of the Keban reservoir. In comparison, the lowest infection prevalence (1.11%) was observed in the 3rd region of the Keban reservoir. The prevalence of Myxobolus sp was higher in trout cage farms in the Keban Dam lake (4.07%) than in the Karakaya reservoir (1.30%). It was determined that Myxobolus sp cysts settle in the capillaries in the secondary gill lamellae of trout and cause pathological disorders such as hypertrophy and hyperplasia. It is important to start routine molecular monitoring of Myxosporean parasite species in cage farms in order to diagnose the exact species and to take precautions before they turn into epidemics.

Engineering sciences, Jul 25, 2022

Although medicinal leeches are used for many years in Azerbaijan for therapeutic purposes, relati... more Although medicinal leeches are used for many years in Azerbaijan for therapeutic purposes, relatively little is still known about their distribution, economic Importance, and general status. The medicinal leech species of Azerbaijan was named Hirudo medicinalis or H. medicinalisorientalis and is still exported under the same names in CITES records. However, within the scope of the study, this medicinal leech species was found to be 100% similar to H. orientalis according to the COI mtDNA gene region. In this study, it was determined that a person could collect an average of 24.19±11.14 medicinal leeches per unit of time and there was 0.60±0.28 leech/m 2 medicinal leech per unit area in the wetlands of Lankaran-Astara region of Azerbaijan. The trade and culture of medicinal leeches in Azerbaijan is a sector that has started in recent years. The country's leech exports in 2021 exceeded the exports of all countries and have increased to around 1498500 units (~1500kg).

The aim study, it was determined the level of hyaluronidase that having a broad range of applicat... more The aim study, it was determined the level of hyaluronidase that having a broad range of applications in medicine and pharmacology. Therefore the level and monthly changes of hyaluronidase were spectrophotometrically investigated in the muscle of medicine leech (Hirudo medicinalis) reared in Cip Fish Reproduction Unit of Fırat University Fisheries Faculty. During the study, the muscle samples from both anterior and posterior parts of total 168 of leeches were dissected and then they were extracted for determination of hyaluronidase levels. While the highest hyaluronidase level was found as 1692.69±40.65µmol/L (1190.49-2270.07) in march, the lowest hyaluronidase activites was found as 322.98±13.41 µmol/L (207.31-824.21) in september. In conclusion, it was determined that the monthly changes of hyaluronidase level in the muscle of medicine leech (Hirudo medicinalis) were found statistically significant (P ≤0.05). As a result after hibernation, gathered leeches are more appropriate than the other periods as did in point of obtaining hyaluronidase from H. medicinalis.

Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 2003

Journal of Fisheriessciences.com, 2008

Türkiye parazitoloji dergisi, 2011

Leeches have been used in the treatment of certain diseases since ancient times. There are a few ... more Leeches have been used in the treatment of certain diseases since ancient times. There are a few species of medicinal leeches. However, H. medicinalis and H. verbana are known in Turkey. Currently, Turkey is one of the world’s most important leech-exporting countries. Secretion of the salivary glands of medical leeches contains more than 100 bioactive substances. These secretions include vasodilators, bacteriostatic, analgesic, anti-inflammatory and anticoagulants, anti-edematous, which eliminate microcirculatory disorders, restore the damaged vascular permeability of tissues and organs, eliminate hypoxia, reduce blood pressure, increase immune system activity, resolving the cause of pain and improve the bioenergetic status of the organism. U.S. Food and Drug Administration (Food and Drug Authority of USA-FDA) have allowed the sale of leeches in this country, the use for plastic surgery, the general purposes and microsurgery in 2004. Turkey is the richest source in terms of the medical leech. Better evaluation of this valuable commodity and more effective use of hirudotherapy in modern medical practice as supportive treatment is very important. (Turkiye Parazitol Derg 2011; 35: 234-9)

Biology Letters, Apr 1, 2023

Parasites in which freshwater snails are intermediate hosts pose a serious threat to human health... more Parasites in which freshwater snails are intermediate hosts pose a serious threat to human health worldwide. We show here that freshwater snails can potentially be controlled by leech predation; in principle, this approach could significantly reduce snail-borne parasitic diseases (SBPDs). Specifically, glossiphoniid leeches, Helobdella austinensis and congener species , consume freshwater snails indiscriminately, while other common leeches do not. A single adult H. austenensis , for example, can consume up to its weight in snails, e.g. Physella acuta, per day. Our predator–prey models suggest that snail populations could be eliminated in relatively short time periods (approximately six months) using a leech biocontrol approach. This could have considerable impact on global SBPDs by breaking the intermediate host life cycle.

DergiPark (Istanbul University), May 3, 2023

The aim of this study was to investigate effects of sulfamerazine on oxidative stress of rainbow ... more The aim of this study was to investigate effects of sulfamerazine on oxidative stress of rainbow trout (Oncorhynchus mykiss). The fish were divided into four groups. The first group was fed with diets containing no sulfamerazine; the second group was supplemented with sulfamerazine at 100 mg kg-1 for 21 days; the third group was treated with sulfamerazine at 200 mg kg-1 for 21 days; fourth group were supplemented with sulfamerazine at 400 kg-1 for 21days. Blood samples were taken to determine of the malondialdehyde (MDA) levels and catalase (CAT) activities from fish on the 3 rd , 7 th , 14 th and 21 st days of feeding. The levels of MDA were lower in the groups exposed to three different doses of sulfamerazine treatment than in the control group on day 3 rd. MDA levels began to increase depending on time at group supplemented with sulfamerazine. MDA levels were found to be higher at fish exposed to 200 mg kg-1 dose than those observed in fish exposed to 100 and 400 mg kg-1 dose of sulfamerazine (P<0.05). CAT activities increased in rainbow trout after application of three different doses of sulfamerazine on day 3 rd of exposure, while they decreased after application of sulfamerazine on day 14 th , 21 st of exposure. CAT activities were found to be higher at fish exposed to 200 mg kg-1 dose of sulfamerazine than those observed in fish exposed to 100 and 400 mg kg-1 dose (P<0.05). It was observed that oxidant-antioxidant status changed in rainbow trout after application of different doses of sulfamerazine.

Etlik veteriner mikrobiyoloji dergisi, Dec 23, 2022

This study was performed to investigate the parasites and pathology in the three different fish s... more This study was performed to investigate the parasites and pathology in the three different fish species, Barbus lacerta, Capoeta trutta and C. umbla, caught in wildlife in Turkey. The fish caught for this study were transported to the laboratory under ethical rules and investigated for parasites. According to the results, two different parasite species (Myxobolus cyprini and Lamproglena pulchella) were morphologically identified. Myxobolus cyprini was found in the soft tissue of the pectoral fins of C. trutta as an off-white, oval cyst. In the histopathological examinations of the cysts, the spores of M. cyprini were observed. Lamproglena pulchella was seen in the gills of B. lacerta. No parasite was also encountered in C. umbla. These parasite species are the first records for wildlife fishes of Uzuncayır Dam Lake of Turkey.

Kemoterapötikler, enfeksiyöz hastalıklarda en önemli sağaltım aracıdır. Sülfonamidler bakteri enf... more Kemoterapötikler, enfeksiyöz hastalıklarda en önemli sağaltım aracıdır. Sülfonamidler bakteri enfeksiyonlarının tedavisinde kullanılan ilk kemoterapötiklerdir. Bu derlemede sülfonamidlerin genel özellikleri ve etki mekanizması, balık hastalıklarında kullanılan sülfonamidlerin türevleri, dozları ve uygulama şekilleriyle ilgili bilgilerin bir araya getirilmesi amaçlanmıştsır.

Aquaculture Reports, Mar 1, 2020

Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and i... more Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg −1) group, TCF-I (11 mg L −1) group, TCF-I (11 mg L −1) plus lycopene (10 mg kg −1) group, TCF-II (22 mg L −1) group and TCF-II (22 mg L −1) plus lycopene (10 mg kg −1) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.

NWSA Academic Journals

The leech samples were collected and identified in some wetlands of Türkiye between January 2009 ... more The leech samples were collected and identified in some wetlands of Türkiye between January 2009 and September 2016. During the study, leech samples were taken from nine wetlands. In the study, seven species belonging to Erpobdellidae (Erpobdella octoculata, Erpobdella testacea), Glossiphonidae (Helobdella stagnalis, Placobdella costata), Hirudinidae (Hirudo sulukii, Hirudo verbana) and Haemopidae (Haemopis sanguisuga) were determined. E. octoculata and E. testacea from Cip Dam Lake and Behramaz stream, from Durusu (Terkos) Lake and Kaledibi Marsh, and H. verbana from Iron H. sanguisuga Marsh and Sülüklü Lake are the first record. H. sulukii and H. verbana leech species are the most important medicinal leech species used in traditional and integrative medicine. Extensive international trade in H. verbana was identified as a major threat to natural populations, so it has been listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES).

NWSA Academic Journals

The article describes the results of the study of leeches in the Fergana Valley, located in the e... more The article describes the results of the study of leeches in the Fergana Valley, located in the eastern part of Uzbekistan. For the first time, the blood-feeding leech Limnatis paluda (Tennent, 1859) was recorded from the study area. It was found that small populations of this species are distributed in ten different biotopes of the Fergana Valley. Distribution coordinates of collected leech samples were determined, and ecological features were studied. Some external and internal morphologies of leeches were compared with species recorded from other regions, and similarities and differences were analyzed. It has been determined that L. paluda feeds on frogs in the wild, and the presence of more than two in a frog is fatal. 107 L. paluda were collected in the study, and it was determined that an average of 10.70±8.22 (range, 2-26) leeches could be collected per unit time. The density of this leech species per unit area in the Fergana Valley region of Uzbekistan was calculated as 0.27±0.21 (range, 0.05-0.65) leech/m 2 .

Türkiye parazitoloji dergisi, Sep 12, 2022

Objective: Leeches are important and reliable indicators of water quality and biodiversity in the... more Objective: Leeches are important and reliable indicators of water quality and biodiversity in the ecosystem, so the presence of specific leech species is often closely related to basic water conditions and the presence of certain animals. This study was carried out in 2017 and 2018 in order to determine the Hirudinea fauna of some wetlands in Bingöl province. The investigation was conducted on a total of 13 stations. Methods: The water parameters of the stations were measured and recorded in situ. The collected specimens were brought alive to the Zoology Laboratory of Bingöl University Biology Department and kept alive under room temperature conditions. The diagnosis of leech samples was made through the living samples, and they were identified at the level of family, genus, and species. Results: During the study, seven species, belonging to six genera and in four families were recorded. These are; Hirudo verbana

Turkish Journal of Fisheries and Aquatic Sciences

Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomi... more Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomid larval stages are significant pathogens that may exert grave effects on both natural fish and aquaculture populations. Diplostomum species, which use fish as a second intermediate host, is one of the most common trematode species, which affects the fish welfare negatively with the formation of cataracts by settling in the eye. This study is determined the molecular characterization of Diplostomum parasite in Cyprinus carpio and Capoeta umbla for the first time from Turkey, based on the mitochondrial COX1 sequence data. Diplostomum samples were determined as Diplostomum spathaceum according to the phylogenetic trees created in the light of the morphological and COX1 sequence data. Sequence results matched ~98-100% with D. spathaceum as a result of blast analysis. It was defined that this parasite was represented by three different haplotypes in Turkey. As a result of haplotype analysis ...

Journal of Limnology and Freshwater Fisheries Research

Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi... more Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp...

Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 2002

FIGURE 9. Maximum Parsimony phylogenetic analysis of COI mtDNA haplotypes (574 total positions). ... more FIGURE 9. Maximum Parsimony phylogenetic analysis of COI mtDNA haplotypes (574 total positions). Tree #1 out of 5 most parsimonious trees (length = 820) is shown. The percentage of replicate trees inwhich the associated taxa clustered in the bootstrap test (500 replicates) are shown next to the branches (Felsenstein 1985). Described species in this study are shown in color, previously unreported sequences are indicated by red diamonds.Remaining sequences were retrieved from GenBank.

FIGURE 8. Representative scanning electron micrographs of the chitonous plate (scute) of Helobdel... more FIGURE 8. Representative scanning electron micrographs of the chitonous plate (scute) of Helobdella stagnalis-like species. A, of Helobdella modesta. B, Helobdella serendipitous n.sp. C, Helobdella n. sp. (Montana).D, Helobdella n. sp. (Oregon).

Engineering sciences, Jan 25, 2024

KAFESLERDE YETİŞTİRİLEN GÖKKUŞAĞI ALABALIĞINDA (Oncorhynchus mykiss) MYXOSPOREAN (MYXOZOA) TÜRLER... more KAFESLERDE YETİŞTİRİLEN GÖKKUŞAĞI ALABALIĞINDA (Oncorhynchus mykiss) MYXOSPOREAN (MYXOZOA) TÜRLERİNİN ARAŞTIRILMASI ÖZ Bu çalışma Elazığ Keban ve Karakaya Baraj göllerinde kafeslerde yetiştiriciliği yapılan gökkuşağı alabalığında (Oncorhynchus mykiss) Myxosporean parazitlerin enfeksiyon ve histopatolojik durumunu belirlemek amacıyla yapılmıştır. Alabalık örnekleri kapasitesi 250 ton ve üzerinde olan ve farklı bölgeleri temsil eden altı kafes işletmesinden Ocak-Aralık 2014 ve Ocak-Aralık 2016 tarihleri arasında iki yıl süreyle toplanmıştır. Parazitolojik ve histopatolojik incelemesi yapılan 1080 gökkuşağı alabalığının 29'unun (%2.69) solungaç lamellalarında Myxobolus sp. tespit edilmiştir. En yüksek enfeksiyon prevalansı (%10.00) Keban baraj gölünün 6. Bölgesinde yer alan alabalık çiftliklerinde belirlenirken, en düşük enfeksiyon prevalansı (%1.11) Keban baraj gölünün 3. Bölgesinde gözlenmiştir. Keban Baraj gölünde yer alan alabalık kafes işletmelerde Myxobolus sp'nin prevalansı (%4.07), Karakaya Baraj Gölünden (%1.30) daha yüksek tespit edilmiştir. Myxobolus sp kistlerinin alabalığın sekonder solungaç lamellalardaki kapillar damarlara yerleştiği, hipertrofi ve hiperplazi gibi patolojik bozukluklar oluşturduğu belirlenmiştir. Myxosporean parazit türlerinin kafes işletmelerinde moleküler olarak rutin takibinin başlatılması kesin tür teşhisi ve salgınlara dönüşmeden önlemlerin alınması açısından önem taşımaktadır. Anahtar Kelimeler: Gökkuşağı Alabalığı, Protozoa, Myxosporean, Myxobolus, Histopatoloji THE STUDY OF MYXOSPOREAN (MYXOZOA) SPECIES IN RAINBOW TROUT (Oncorhynchus mykiss) FARMED IN CAGES ABSTRACT This study was conducted to determine the infection and histopathological status of Myxosporean parasites in rainbow trout (Oncorhynchus mykiss) cultured in cages in Elazığ Keban and Karakaya reservoirs. Rainbow trout samples were collected from six cage farms with a capacity of 250 tons or more and representing different regions for two years between January-December 2014 and January-December 2016. Myxobolus sp. was detected in the gill lamellae of 29 (2.69%) out of 1080 rainbow trout that were parasitologically and histopathologically examined. The highest infection prevalence (10.00%) was determined in trout farms located in the 6th region of the Keban reservoir. In comparison, the lowest infection prevalence (1.11%) was observed in the 3rd region of the Keban reservoir. The prevalence of Myxobolus sp was higher in trout cage farms in the Keban Dam lake (4.07%) than in the Karakaya reservoir (1.30%). It was determined that Myxobolus sp cysts settle in the capillaries in the secondary gill lamellae of trout and cause pathological disorders such as hypertrophy and hyperplasia. It is important to start routine molecular monitoring of Myxosporean parasite species in cage farms in order to diagnose the exact species and to take precautions before they turn into epidemics.

Engineering sciences, Jul 25, 2022

Although medicinal leeches are used for many years in Azerbaijan for therapeutic purposes, relati... more Although medicinal leeches are used for many years in Azerbaijan for therapeutic purposes, relatively little is still known about their distribution, economic Importance, and general status. The medicinal leech species of Azerbaijan was named Hirudo medicinalis or H. medicinalisorientalis and is still exported under the same names in CITES records. However, within the scope of the study, this medicinal leech species was found to be 100% similar to H. orientalis according to the COI mtDNA gene region. In this study, it was determined that a person could collect an average of 24.19±11.14 medicinal leeches per unit of time and there was 0.60±0.28 leech/m 2 medicinal leech per unit area in the wetlands of Lankaran-Astara region of Azerbaijan. The trade and culture of medicinal leeches in Azerbaijan is a sector that has started in recent years. The country's leech exports in 2021 exceeded the exports of all countries and have increased to around 1498500 units (~1500kg).

The aim study, it was determined the level of hyaluronidase that having a broad range of applicat... more The aim study, it was determined the level of hyaluronidase that having a broad range of applications in medicine and pharmacology. Therefore the level and monthly changes of hyaluronidase were spectrophotometrically investigated in the muscle of medicine leech (Hirudo medicinalis) reared in Cip Fish Reproduction Unit of Fırat University Fisheries Faculty. During the study, the muscle samples from both anterior and posterior parts of total 168 of leeches were dissected and then they were extracted for determination of hyaluronidase levels. While the highest hyaluronidase level was found as 1692.69±40.65µmol/L (1190.49-2270.07) in march, the lowest hyaluronidase activites was found as 322.98±13.41 µmol/L (207.31-824.21) in september. In conclusion, it was determined that the monthly changes of hyaluronidase level in the muscle of medicine leech (Hirudo medicinalis) were found statistically significant (P ≤0.05). As a result after hibernation, gathered leeches are more appropriate than the other periods as did in point of obtaining hyaluronidase from H. medicinalis.

Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 2003

Journal of Fisheriessciences.com, 2008

Türkiye parazitoloji dergisi, 2011

Leeches have been used in the treatment of certain diseases since ancient times. There are a few ... more Leeches have been used in the treatment of certain diseases since ancient times. There are a few species of medicinal leeches. However, H. medicinalis and H. verbana are known in Turkey. Currently, Turkey is one of the world’s most important leech-exporting countries. Secretion of the salivary glands of medical leeches contains more than 100 bioactive substances. These secretions include vasodilators, bacteriostatic, analgesic, anti-inflammatory and anticoagulants, anti-edematous, which eliminate microcirculatory disorders, restore the damaged vascular permeability of tissues and organs, eliminate hypoxia, reduce blood pressure, increase immune system activity, resolving the cause of pain and improve the bioenergetic status of the organism. U.S. Food and Drug Administration (Food and Drug Authority of USA-FDA) have allowed the sale of leeches in this country, the use for plastic surgery, the general purposes and microsurgery in 2004. Turkey is the richest source in terms of the medical leech. Better evaluation of this valuable commodity and more effective use of hirudotherapy in modern medical practice as supportive treatment is very important. (Turkiye Parazitol Derg 2011; 35: 234-9)

Biology Letters, Apr 1, 2023

Parasites in which freshwater snails are intermediate hosts pose a serious threat to human health... more Parasites in which freshwater snails are intermediate hosts pose a serious threat to human health worldwide. We show here that freshwater snails can potentially be controlled by leech predation; in principle, this approach could significantly reduce snail-borne parasitic diseases (SBPDs). Specifically, glossiphoniid leeches, Helobdella austinensis and congener species , consume freshwater snails indiscriminately, while other common leeches do not. A single adult H. austenensis , for example, can consume up to its weight in snails, e.g. Physella acuta, per day. Our predator–prey models suggest that snail populations could be eliminated in relatively short time periods (approximately six months) using a leech biocontrol approach. This could have considerable impact on global SBPDs by breaking the intermediate host life cycle.

DergiPark (Istanbul University), May 3, 2023

The aim of this study was to investigate effects of sulfamerazine on oxidative stress of rainbow ... more The aim of this study was to investigate effects of sulfamerazine on oxidative stress of rainbow trout (Oncorhynchus mykiss). The fish were divided into four groups. The first group was fed with diets containing no sulfamerazine; the second group was supplemented with sulfamerazine at 100 mg kg-1 for 21 days; the third group was treated with sulfamerazine at 200 mg kg-1 for 21 days; fourth group were supplemented with sulfamerazine at 400 kg-1 for 21days. Blood samples were taken to determine of the malondialdehyde (MDA) levels and catalase (CAT) activities from fish on the 3 rd , 7 th , 14 th and 21 st days of feeding. The levels of MDA were lower in the groups exposed to three different doses of sulfamerazine treatment than in the control group on day 3 rd. MDA levels began to increase depending on time at group supplemented with sulfamerazine. MDA levels were found to be higher at fish exposed to 200 mg kg-1 dose than those observed in fish exposed to 100 and 400 mg kg-1 dose of sulfamerazine (P<0.05). CAT activities increased in rainbow trout after application of three different doses of sulfamerazine on day 3 rd of exposure, while they decreased after application of sulfamerazine on day 14 th , 21 st of exposure. CAT activities were found to be higher at fish exposed to 200 mg kg-1 dose of sulfamerazine than those observed in fish exposed to 100 and 400 mg kg-1 dose (P<0.05). It was observed that oxidant-antioxidant status changed in rainbow trout after application of different doses of sulfamerazine.

Etlik veteriner mikrobiyoloji dergisi, Dec 23, 2022

This study was performed to investigate the parasites and pathology in the three different fish s... more This study was performed to investigate the parasites and pathology in the three different fish species, Barbus lacerta, Capoeta trutta and C. umbla, caught in wildlife in Turkey. The fish caught for this study were transported to the laboratory under ethical rules and investigated for parasites. According to the results, two different parasite species (Myxobolus cyprini and Lamproglena pulchella) were morphologically identified. Myxobolus cyprini was found in the soft tissue of the pectoral fins of C. trutta as an off-white, oval cyst. In the histopathological examinations of the cysts, the spores of M. cyprini were observed. Lamproglena pulchella was seen in the gills of B. lacerta. No parasite was also encountered in C. umbla. These parasite species are the first records for wildlife fishes of Uzuncayır Dam Lake of Turkey.

Kemoterapötikler, enfeksiyöz hastalıklarda en önemli sağaltım aracıdır. Sülfonamidler bakteri enf... more Kemoterapötikler, enfeksiyöz hastalıklarda en önemli sağaltım aracıdır. Sülfonamidler bakteri enfeksiyonlarının tedavisinde kullanılan ilk kemoterapötiklerdir. Bu derlemede sülfonamidlerin genel özellikleri ve etki mekanizması, balık hastalıklarında kullanılan sülfonamidlerin türevleri, dozları ve uygulama şekilleriyle ilgili bilgilerin bir araya getirilmesi amaçlanmıştsır.

Aquaculture Reports, Mar 1, 2020

Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and i... more Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg −1) group, TCF-I (11 mg L −1) group, TCF-I (11 mg L −1) plus lycopene (10 mg kg −1) group, TCF-II (22 mg L −1) group and TCF-II (22 mg L −1) plus lycopene (10 mg kg −1) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.

NWSA Academic Journals

The leech samples were collected and identified in some wetlands of Türkiye between January 2009 ... more The leech samples were collected and identified in some wetlands of Türkiye between January 2009 and September 2016. During the study, leech samples were taken from nine wetlands. In the study, seven species belonging to Erpobdellidae (Erpobdella octoculata, Erpobdella testacea), Glossiphonidae (Helobdella stagnalis, Placobdella costata), Hirudinidae (Hirudo sulukii, Hirudo verbana) and Haemopidae (Haemopis sanguisuga) were determined. E. octoculata and E. testacea from Cip Dam Lake and Behramaz stream, from Durusu (Terkos) Lake and Kaledibi Marsh, and H. verbana from Iron H. sanguisuga Marsh and Sülüklü Lake are the first record. H. sulukii and H. verbana leech species are the most important medicinal leech species used in traditional and integrative medicine. Extensive international trade in H. verbana was identified as a major threat to natural populations, so it has been listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES).

NWSA Academic Journals

The article describes the results of the study of leeches in the Fergana Valley, located in the e... more The article describes the results of the study of leeches in the Fergana Valley, located in the eastern part of Uzbekistan. For the first time, the blood-feeding leech Limnatis paluda (Tennent, 1859) was recorded from the study area. It was found that small populations of this species are distributed in ten different biotopes of the Fergana Valley. Distribution coordinates of collected leech samples were determined, and ecological features were studied. Some external and internal morphologies of leeches were compared with species recorded from other regions, and similarities and differences were analyzed. It has been determined that L. paluda feeds on frogs in the wild, and the presence of more than two in a frog is fatal. 107 L. paluda were collected in the study, and it was determined that an average of 10.70±8.22 (range, 2-26) leeches could be collected per unit time. The density of this leech species per unit area in the Fergana Valley region of Uzbekistan was calculated as 0.27±0.21 (range, 0.05-0.65) leech/m 2 .

Türkiye parazitoloji dergisi, Sep 12, 2022

Objective: Leeches are important and reliable indicators of water quality and biodiversity in the... more Objective: Leeches are important and reliable indicators of water quality and biodiversity in the ecosystem, so the presence of specific leech species is often closely related to basic water conditions and the presence of certain animals. This study was carried out in 2017 and 2018 in order to determine the Hirudinea fauna of some wetlands in Bingöl province. The investigation was conducted on a total of 13 stations. Methods: The water parameters of the stations were measured and recorded in situ. The collected specimens were brought alive to the Zoology Laboratory of Bingöl University Biology Department and kept alive under room temperature conditions. The diagnosis of leech samples was made through the living samples, and they were identified at the level of family, genus, and species. Results: During the study, seven species, belonging to six genera and in four families were recorded. These are; Hirudo verbana

Turkish Journal of Fisheries and Aquatic Sciences

Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomi... more Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomid larval stages are significant pathogens that may exert grave effects on both natural fish and aquaculture populations. Diplostomum species, which use fish as a second intermediate host, is one of the most common trematode species, which affects the fish welfare negatively with the formation of cataracts by settling in the eye. This study is determined the molecular characterization of Diplostomum parasite in Cyprinus carpio and Capoeta umbla for the first time from Turkey, based on the mitochondrial COX1 sequence data. Diplostomum samples were determined as Diplostomum spathaceum according to the phylogenetic trees created in the light of the morphological and COX1 sequence data. Sequence results matched ~98-100% with D. spathaceum as a result of blast analysis. It was defined that this parasite was represented by three different haplotypes in Turkey. As a result of haplotype analysis ...

Journal of Limnology and Freshwater Fisheries Research

Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi... more Bu çalışma, Ocak 2012- Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine göre birim zaman, birim çerçeve (Sülük toplama çerçevesi) ve Ekman kepçesi yöntemiyle toplandı. Toplam 169 sulak alanın 53’ünde sülük tespit edildi. Çalışma süresince toplanan sülük örnekleri su bulunan 0,5-1,0 L’lik pet bidonlarla laboratuvara taşındı. Sülük örnekleri önce % 10 etanol içinde bayıltıldı, sonra %70 alkol veya %4 formalin içinde tespit edildi. Toplanmış olan sülükler morfolojik özelliklerine göre stereo mikroskop altında teşhisi yapıldı. Bu çalışma sonunda , Güneydoğu Anadolu Bölgesi sulak alanlarında Albolossiphonia heteroclita, Albolossiphonia sp., Dina apathyi, Dina sp., Erpobdella octoculata, E. testacea, Erpobdella sp...

Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 2002

FIGURE 9. Maximum Parsimony phylogenetic analysis of COI mtDNA haplotypes (574 total positions). ... more FIGURE 9. Maximum Parsimony phylogenetic analysis of COI mtDNA haplotypes (574 total positions). Tree #1 out of 5 most parsimonious trees (length = 820) is shown. The percentage of replicate trees inwhich the associated taxa clustered in the bootstrap test (500 replicates) are shown next to the branches (Felsenstein 1985). Described species in this study are shown in color, previously unreported sequences are indicated by red diamonds.Remaining sequences were retrieved from GenBank.

FIGURE 8. Representative scanning electron micrographs of the chitonous plate (scute) of Helobdel... more FIGURE 8. Representative scanning electron micrographs of the chitonous plate (scute) of Helobdella stagnalis-like species. A, of Helobdella modesta. B, Helobdella serendipitous n.sp. C, Helobdella n. sp. (Montana).D, Helobdella n. sp. (Oregon).