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Papers by Natalia Villate
Ophthalmology, Feb 1, 2001
PurposeTo evaluate the quality of reporting of all the new randomized clinical trials published d... more PurposeTo evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in Ophthalmology using the CONSORT statement parameters.
Investigative Ophthalmology & Visual Science, 2003
Investigative Ophthalmology & Visual Science, May 1, 2003
Investigative Ophthalmology & Visual Science, Dec 1, 2002
Investigative Ophthalmology & Visual Science, 2003
Investigative Ophthalmology & Visual Science, 2004
Investigative Ophthalmology & Visual Science, Dec 1, 2002
American Journal of Ophthalmology Case Reports
JAMA Ophthalmology, 2019
The presubiculum plays a key role in processing and integrating spatial and head-directional info... more The presubiculum plays a key role in processing and integrating spatial and head-directional information. Layer III neurons of the presubiculum provide strong projections to the superficial layers of the medial entorhinal cortex (MEC) in the rat. Our previous study revealed that the terminal distribution of efferents from layer III cells of the presubiculum was organized in a band-like fashion within the MEC, and the transverse axis of these zones ran parallel to the rhinal fissure. Identifying axonal branching patterns of layer III neurons of the presubiculum is important to further elucidate the functional roles of the presubiculum. In the present study, we visualized all axonal processes and terminal distributions of single presubicular layer III neurons in the rat, using in vivo injection of a viral vector expressing membrane-targeted palmitoylation site-attached green fluorescent protein (GFP). We found that layer III of the rat presubiculum comprised multiple types of neurons (n = 12) with characteristic patterns of axonal collateralization, including cortical projection neurons (n = 6) and several types of intrinsic connectional neurons (n = 6). Two of six cortical projection neurons provided two or three major axonal branches to the MEC and formed elaborate terminal arbors within the superficial layers of the MEC. The width and axis of the area of their terminal distribution resembled that of the band-like terminal field seen in our massive-scale observation. Two of the other four cortical projection neurons gave off axonal branches to the MEC and also to the subiculum, and each of the other two neurons sent axons to the subiculum or parasubiculum. Patterns of axonal arborization of six intrinsic connectional neurons were distinct from each other, with four neurons sending many axonal branches to both superficial and deep layers of the presubiculum and the other two neurons showing sparse axonal branches with terminations confined to layers III-V of the presubiculum. These data demonstrate that layer III of the rat presubiculum consists of multiple types of cortical projection neurons and interneurons, and also suggest that inputs from a single presubicular layer III neuron can directly affect a band-like zone of the MEC.
Cornea, 2005
To report an eye-threatening complication associated with amniotic membrane grafting. Interventio... more To report an eye-threatening complication associated with amniotic membrane grafting. Interventional case report. Retrospective review of the clinical and surgical records of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Melting of a corneal graft after amniotic membrane transplantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tarsorraphy and systemic steroids. To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.
Ophthalmology, 2001
To evaluate the quality of reporting of all the new randomized clinical trials published during 1... more To evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in OPHTHALMOLOGY: using the CONSORT statement parameters. Descriptive series of published studies. Randomized clinical trials. Eligible studies were all randomized clinical trials published in Volume 106 (1999) of OPHTHALMOLOGY: that reported the results of prospectively conducted studies in humans comparing two or more therapeutic options with regard to their effect on one or various outcomes, in which the authors stated that a process of randomization or quasi-randomization was performed before the interventions. Each study was evaluated by two independent observers establishing the specific report in the published manuscript of 57 CONSORT statement descriptors, and consensus was obtained between evaluators. Overall presence of CONSORT statement descriptors in published reports. The mean number of positive CONSORT descriptors for the 24 eligible randomized clinical trials was 33.42 (standard error of the mean [SEM] 1.57) of 57 possible. This score was significantly different (P: < 0.001) from the score of 16.6 (standard error of the mean 0.6) reported for OPHTHALMOLOGY: in the 1991 to 1994 study by Scherer and Crawley. Of the nine descriptors measuring the quality of abstract reporting, a mean of 6.25 (SEM 0.3) was included in the 24 study abstracts evaluated, significantly different from the Scherer and Crawley score (5.2, SEM 0.2. P: < 0.001). Four and six descriptors increased and decreased, respectively, their reporting in more than 20%. Key descriptors such as hypothesis postulation, primary outcome determination, sample size calculations, and a discussion of the limitations of the study such as bias, imprecision, and internal validity are still reported in less than 42% of clinical trials. This study describes for the first time after the adoption of the CONSORT statement recommendations the status on the quality of reporting of recently published randomized clinical trials in OPHTHALMOLOGY: Although not a direct measure of the intrinsic quality of a study, reporting quality provides the reader with useful tools for the evaluation of its validity. The quality of reporting of randomized clinical trials shows an overall improvement when compared with the period between 1991 and 1994. However, there is still significant room for improvement, especially in descriptors essential for the validation of clinical trial results that are still widely underreported.
A 45-year-old man presented with floaters in his left eye and headaches for 2 months. He experien... more A 45-year-old man presented with floaters in his left eye and headaches for 2 months. He experienced left temporal field loss when lifting heavy objects. He took no medications and denied prior surgery, eye trauma, or pain. His best-corrected visual acuity was 20/25 OD and 20/30 OS. The only abnormalities on bilateral eye examination were 1+ levels of anterior vitreous cells and trace levels of posterior vitreous cells in the left eye, without vitreous haze, and a left retinal detachment extending from 1:30 to 10:00 o'clock, with macular involvement and without visible retinal tears. There were 360° left choroidal detachments. Optical coherence tomography showed choroidal thickening in both maculae. Fluo-rescein angiography (FA) of the right eye gave normal results. Figure 1A depicts the FA image of the left eye. On B-scan ultrasonography, there was a subclinical choroidal detachment superonasally in the right eye. An ultrasonographic image confirmed the detachments in the left eye (Figure 1B). The findings of a prior magnetic resonance image of the brain, chest radiographic image, and computed tomographic image of the chest were unremark-able. Test results for syphilis, tuberculosis, bartonella, and toxoplasma were negative, as were test results for inflammatory markers, quantitative serum proteins, and immunoglobulins. The patient started receiving 60 mg of oral prednisone daily. Three weeks later, his best-corrected visual acuity worsened to 20/50 OS. A posterior sub-Tenon injection of 40 mg of triamcinolone acetonide was given, along with tapering doses of oral prednisone for 6 more weeks. There was further decline in visual acuity to 20/60 OS and worsening subretinal fluid. Diagnosis Type 3 uveal effusion syndrome What to Do Next B. Surgery to create scleral windows Discussion Diagnostic vitrectomy with cytologic testing (choice A) would be appropriate for suspected primary vitreoretinal lymphoma with so-called leopard-spot lymphomatous infiltration under the retinal pigment epithelium, but there were few vitreous cells seen in this Fluorescein angiographic image A B-scan ultrasonographic image B Figure 1. Images of the left eye at baseline. A, Midphase fluorescein angiography of the left eye. There are multiple peripheral areas of punctate hyperfluorescence. Leakage is more pronounced posteriorly around the nerve. There is scattered blockage of background fluorescence owing to retinal pigment epithelium mottling, which creates a leopard-spot pattern. A retinal detachment is seen inferiorly with tortuous vessels and nonfluorescent subretinal fluid. B, A B-scan ultrasonographic image of the left eye in the longitudinal 6 o'clock meridian demonstrates retinal detachment and a nonserous choroidal detachment superiorly. The optic nerve shadow is seen inferiorly. There is no fluid in the Tenon capsule. The detachment was mapped to extend from 1:30 to 10:00 o'clock and involve the macula. The maximum height of the choroidal detachment was 3.4 mm. No retinal tear was visualized. WHAT WOULD YOU DO NEXT?
Purpose: To report an eye-threatening complication associated with amniotic membrane grafting. of... more Purpose: To report an eye-threatening complication associated with amniotic membrane grafting. of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Results: Melting of a corneal graft after amniotic membrane trans-plantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tar-sorraphy and systemic steroids. Conclusions: To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.
Data Revues 00029394 V136i4 S0002939403004264, Aug 26, 2011
Ophthalmology, 2001
To evaluate the quality of reporting of all the new randomized clinical trials published during 1... more To evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in OPHTHALMOLOGY: using the CONSORT statement parameters. Descriptive series of published studies. Randomized clinical trials. Eligible studies were all randomized clinical trials published in Volume 106 (1999) of OPHTHALMOLOGY: that reported the results of prospectively conducted studies in humans comparing two or more therapeutic options with regard to their effect on one or various outcomes, in which the authors stated that a process of randomization or quasi-randomization was performed before the interventions. Each study was evaluated by two independent observers establishing the specific report in the published manuscript of 57 CONSORT statement descriptors, and consensus was obtained between evaluators. Overall presence of CONSORT statement descriptors in published reports. The mean number of positive CONSORT descriptors for the 24 eligible randomized clinical trials was 33.42...
American Journal of Ophthalmology, 2003
Cornea, 2005
To report an eye-threatening complication associated with amniotic membrane grafting. Interventio... more To report an eye-threatening complication associated with amniotic membrane grafting. Interventional case report. Retrospective review of the clinical and surgical records of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Melting of a corneal graft after amniotic membrane transplantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tarsorraphy and systemic steroids. To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.
Ophthalmology, Feb 1, 2001
PurposeTo evaluate the quality of reporting of all the new randomized clinical trials published d... more PurposeTo evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in Ophthalmology using the CONSORT statement parameters.
Investigative Ophthalmology & Visual Science, 2003
Investigative Ophthalmology & Visual Science, May 1, 2003
Investigative Ophthalmology & Visual Science, Dec 1, 2002
Investigative Ophthalmology & Visual Science, 2003
Investigative Ophthalmology & Visual Science, 2004
Investigative Ophthalmology & Visual Science, Dec 1, 2002
American Journal of Ophthalmology Case Reports
JAMA Ophthalmology, 2019
The presubiculum plays a key role in processing and integrating spatial and head-directional info... more The presubiculum plays a key role in processing and integrating spatial and head-directional information. Layer III neurons of the presubiculum provide strong projections to the superficial layers of the medial entorhinal cortex (MEC) in the rat. Our previous study revealed that the terminal distribution of efferents from layer III cells of the presubiculum was organized in a band-like fashion within the MEC, and the transverse axis of these zones ran parallel to the rhinal fissure. Identifying axonal branching patterns of layer III neurons of the presubiculum is important to further elucidate the functional roles of the presubiculum. In the present study, we visualized all axonal processes and terminal distributions of single presubicular layer III neurons in the rat, using in vivo injection of a viral vector expressing membrane-targeted palmitoylation site-attached green fluorescent protein (GFP). We found that layer III of the rat presubiculum comprised multiple types of neurons (n = 12) with characteristic patterns of axonal collateralization, including cortical projection neurons (n = 6) and several types of intrinsic connectional neurons (n = 6). Two of six cortical projection neurons provided two or three major axonal branches to the MEC and formed elaborate terminal arbors within the superficial layers of the MEC. The width and axis of the area of their terminal distribution resembled that of the band-like terminal field seen in our massive-scale observation. Two of the other four cortical projection neurons gave off axonal branches to the MEC and also to the subiculum, and each of the other two neurons sent axons to the subiculum or parasubiculum. Patterns of axonal arborization of six intrinsic connectional neurons were distinct from each other, with four neurons sending many axonal branches to both superficial and deep layers of the presubiculum and the other two neurons showing sparse axonal branches with terminations confined to layers III-V of the presubiculum. These data demonstrate that layer III of the rat presubiculum consists of multiple types of cortical projection neurons and interneurons, and also suggest that inputs from a single presubicular layer III neuron can directly affect a band-like zone of the MEC.
Cornea, 2005
To report an eye-threatening complication associated with amniotic membrane grafting. Interventio... more To report an eye-threatening complication associated with amniotic membrane grafting. Interventional case report. Retrospective review of the clinical and surgical records of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Melting of a corneal graft after amniotic membrane transplantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tarsorraphy and systemic steroids. To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.
Ophthalmology, 2001
To evaluate the quality of reporting of all the new randomized clinical trials published during 1... more To evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in OPHTHALMOLOGY: using the CONSORT statement parameters. Descriptive series of published studies. Randomized clinical trials. Eligible studies were all randomized clinical trials published in Volume 106 (1999) of OPHTHALMOLOGY: that reported the results of prospectively conducted studies in humans comparing two or more therapeutic options with regard to their effect on one or various outcomes, in which the authors stated that a process of randomization or quasi-randomization was performed before the interventions. Each study was evaluated by two independent observers establishing the specific report in the published manuscript of 57 CONSORT statement descriptors, and consensus was obtained between evaluators. Overall presence of CONSORT statement descriptors in published reports. The mean number of positive CONSORT descriptors for the 24 eligible randomized clinical trials was 33.42 (standard error of the mean [SEM] 1.57) of 57 possible. This score was significantly different (P: < 0.001) from the score of 16.6 (standard error of the mean 0.6) reported for OPHTHALMOLOGY: in the 1991 to 1994 study by Scherer and Crawley. Of the nine descriptors measuring the quality of abstract reporting, a mean of 6.25 (SEM 0.3) was included in the 24 study abstracts evaluated, significantly different from the Scherer and Crawley score (5.2, SEM 0.2. P: < 0.001). Four and six descriptors increased and decreased, respectively, their reporting in more than 20%. Key descriptors such as hypothesis postulation, primary outcome determination, sample size calculations, and a discussion of the limitations of the study such as bias, imprecision, and internal validity are still reported in less than 42% of clinical trials. This study describes for the first time after the adoption of the CONSORT statement recommendations the status on the quality of reporting of recently published randomized clinical trials in OPHTHALMOLOGY: Although not a direct measure of the intrinsic quality of a study, reporting quality provides the reader with useful tools for the evaluation of its validity. The quality of reporting of randomized clinical trials shows an overall improvement when compared with the period between 1991 and 1994. However, there is still significant room for improvement, especially in descriptors essential for the validation of clinical trial results that are still widely underreported.
A 45-year-old man presented with floaters in his left eye and headaches for 2 months. He experien... more A 45-year-old man presented with floaters in his left eye and headaches for 2 months. He experienced left temporal field loss when lifting heavy objects. He took no medications and denied prior surgery, eye trauma, or pain. His best-corrected visual acuity was 20/25 OD and 20/30 OS. The only abnormalities on bilateral eye examination were 1+ levels of anterior vitreous cells and trace levels of posterior vitreous cells in the left eye, without vitreous haze, and a left retinal detachment extending from 1:30 to 10:00 o'clock, with macular involvement and without visible retinal tears. There were 360° left choroidal detachments. Optical coherence tomography showed choroidal thickening in both maculae. Fluo-rescein angiography (FA) of the right eye gave normal results. Figure 1A depicts the FA image of the left eye. On B-scan ultrasonography, there was a subclinical choroidal detachment superonasally in the right eye. An ultrasonographic image confirmed the detachments in the left eye (Figure 1B). The findings of a prior magnetic resonance image of the brain, chest radiographic image, and computed tomographic image of the chest were unremark-able. Test results for syphilis, tuberculosis, bartonella, and toxoplasma were negative, as were test results for inflammatory markers, quantitative serum proteins, and immunoglobulins. The patient started receiving 60 mg of oral prednisone daily. Three weeks later, his best-corrected visual acuity worsened to 20/50 OS. A posterior sub-Tenon injection of 40 mg of triamcinolone acetonide was given, along with tapering doses of oral prednisone for 6 more weeks. There was further decline in visual acuity to 20/60 OS and worsening subretinal fluid. Diagnosis Type 3 uveal effusion syndrome What to Do Next B. Surgery to create scleral windows Discussion Diagnostic vitrectomy with cytologic testing (choice A) would be appropriate for suspected primary vitreoretinal lymphoma with so-called leopard-spot lymphomatous infiltration under the retinal pigment epithelium, but there were few vitreous cells seen in this Fluorescein angiographic image A B-scan ultrasonographic image B Figure 1. Images of the left eye at baseline. A, Midphase fluorescein angiography of the left eye. There are multiple peripheral areas of punctate hyperfluorescence. Leakage is more pronounced posteriorly around the nerve. There is scattered blockage of background fluorescence owing to retinal pigment epithelium mottling, which creates a leopard-spot pattern. A retinal detachment is seen inferiorly with tortuous vessels and nonfluorescent subretinal fluid. B, A B-scan ultrasonographic image of the left eye in the longitudinal 6 o'clock meridian demonstrates retinal detachment and a nonserous choroidal detachment superiorly. The optic nerve shadow is seen inferiorly. There is no fluid in the Tenon capsule. The detachment was mapped to extend from 1:30 to 10:00 o'clock and involve the macula. The maximum height of the choroidal detachment was 3.4 mm. No retinal tear was visualized. WHAT WOULD YOU DO NEXT?
Purpose: To report an eye-threatening complication associated with amniotic membrane grafting. of... more Purpose: To report an eye-threatening complication associated with amniotic membrane grafting. of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Results: Melting of a corneal graft after amniotic membrane trans-plantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tar-sorraphy and systemic steroids. Conclusions: To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.
Data Revues 00029394 V136i4 S0002939403004264, Aug 26, 2011
Ophthalmology, 2001
To evaluate the quality of reporting of all the new randomized clinical trials published during 1... more To evaluate the quality of reporting of all the new randomized clinical trials published during 1999 in OPHTHALMOLOGY: using the CONSORT statement parameters. Descriptive series of published studies. Randomized clinical trials. Eligible studies were all randomized clinical trials published in Volume 106 (1999) of OPHTHALMOLOGY: that reported the results of prospectively conducted studies in humans comparing two or more therapeutic options with regard to their effect on one or various outcomes, in which the authors stated that a process of randomization or quasi-randomization was performed before the interventions. Each study was evaluated by two independent observers establishing the specific report in the published manuscript of 57 CONSORT statement descriptors, and consensus was obtained between evaluators. Overall presence of CONSORT statement descriptors in published reports. The mean number of positive CONSORT descriptors for the 24 eligible randomized clinical trials was 33.42...
American Journal of Ophthalmology, 2003
Cornea, 2005
To report an eye-threatening complication associated with amniotic membrane grafting. Interventio... more To report an eye-threatening complication associated with amniotic membrane grafting. Interventional case report. Retrospective review of the clinical and surgical records of a 43-year-old man who developed melting of his corneal graft after amniotic membrane transplantation. Melting of a corneal graft after amniotic membrane transplantation was arrested by utilizing a tectonic, full-thickness corneal button denuded of endothelium in conjunction with a temporary tarsorraphy and systemic steroids. To the best of our knowledge, this is the first report of an eye-threatening complication associated with amniotic membrane grafting. Caution should be exercised in utilizing amniotic membrane in patients who have undergone multiple ophthalmologic surgical procedures, which may sensitize the ocular immune system or lead to localized ischemia.