Nadia Shaban - Academia.edu (original) (raw)
Papers by Nadia Shaban
Environmental Science and Pollution Research
The present study was done to evaluate the protective and therapeutic role of mango pulp (M), epr... more The present study was done to evaluate the protective and therapeutic role of mango pulp (M), eprosartan drug (E), and their co-administration (EM) against hepatotoxicity induced by thioacetamide (T). Seven groups of rats were prepared as follows: the control (C) group (normal rats), T group (the rats were injected with T), T-M group (the rats were injected with T, and then treated with M), T-E group (the rats were injected with T, and then treated with E), T-EM group (the rats were injected with T, and then treated with E and M), M-TM-M group (the rats were administered with M before, during, and after T injection), and M group (the healthy rats were administered with M only). Firstly, the characterizations of M were determined. Also, the markers of hepatic oxidative stress [malondialdehyde (MDA) and glutathione (GSH) levels and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GSR)], inflammation and fibrosis [(tumor necrosis fa...
Many manuscripts and historical books contain a form of deterioration known as foxing or fox spot... more Many manuscripts and historical books contain a form of deterioration known as foxing or fox spots, a brownish stain which has the effect of altering aesthetic and visual appeal. The aim of this work is to study the role of the extracts of Water Cantaloupe (CE) and Water melon (WE) separately as natural products in removing foxing spots in various modern and old papers. Old papers and three types of modern papers made from cotton, linen and a mixture of cotton, linen and wood (1:1:1) were used for this purpose. Each type was divided into two groups, one of them was infected with foxing and the other was left as control (uninfected). Infected papers were treated with CE, WE and 2% sodium hypochlorite (as a traditional chemical bleaching) separately. Fourier Transform Infrared Spectroscopy (FTIR), Atomic Absorption Spectroscopy, Scanning Electron Microscopy equipped with Energy-Dispersive X-ray spectroscopy (SEM-EDX) in addition to some optical and mechanical properties were carried o...
Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In... more Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In our previous studies, dithiophenolato titanium (IV) Complex "DBT" and its chitosan nanocomposite "DBT-CSNPs" were prepared and we showed that these compounds have antibacterial activities, cytotoxic, and have abilities to bind with DNA helixes. Therefore, in this study, we evaluated the LD50 values of dithiophenolato titanium (IV)-complex (DBT) and its high thermal stable chitosan nanoparticles (DBT-CSNPs). Then their therapeutic effects against liver injuries induced by carbon tetrachloride (CCl4) were assessed and compared with cisplatin treatment. Additionally, the anti-proliferative activity of DBT and DBT-CSNPs against human liver cancer (HepG2) cell lines through the analysis of the cell cycle was evaluated. Methods Nine groups of rats were prepared: normal, DBT, DBT-CSNPs, CSNPs, CCl4, CCl4-DBT, CCl4-DBT-CSNPs, CCl4-CSNPs and CCl4-cisplatin. Liver histopatholo...
Asian Pacific Journal of Cancer Prevention, 2015
The effect of Eruca sativa seed extract (SE) on nuclear factor kappa B (NF-κB), cyclooxygenase-2 ... more The effect of Eruca sativa seed extract (SE) on nuclear factor kappa B (NF-κB), cyclooxygenase-2 (COX-2) and B-cell lymphoma-2 (Bcl-2) gene expression levels was investigated in rat mammary gland carcinogenesis induced by 7,12 dimethylbenz(α)anthracene (DMBA). DMBA increased NF-κB, COX-2 and Bcl-2 gene expression levels and lipid peroxidation (LP), while, decreased glutathione-S-transferase (GST) and superoxide dismutase (SOD) activities and total antioxidant concentration (TAC) compared to the control group. After DMBA administration, SE treatment reduced NF-κB, COX-2 and Bcl-2 gene expression levels and LP. Hence, SE treatment reduced inflammation and cell proliferation, while increasing apoptosis, GST and SOD activities and TAC. Analysis revealed that SE has high concentrations of total flavonoids, triterpenoids, alkaloids and polyphenolic compounds such as gallic, chlorogenic, caffeic, 3,4-dicaffeoyl quinic, 3,5-dicaffeoyl quinic, tannic, cinnamic acids, catechin and phloridzin....
Abstract: The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzot... more Abstract: The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-carboxamide-1,1-dioxide.) on lipoxygenase enzyme (LOX) in different tissues of rabbits in vitro and in vivo were studied. MLX (117 nM) inhibited trachea, skin, stomach and serum LOX enzyme activities in vitro with varying inhibitory degrees. The administration of MLX (0.214 mg/kg body mass/day for 7days) inhibited trachea, skin and stomach LOX activities. However, MLX had no effect on serum LOX activity. Linweaver-Burk plots showed that MLX inhibited LOX enzyme in vitro and in vivo by competitive inhibition manner. The values of Km and Vmax in the absence and presence of MLX were determined.
Saudi Journal of Medical and Pharmaceutical Sciences
This study highlights the characterization and biological activities (in vitro) of root extracts ... more This study highlights the characterization and biological activities (in vitro) of root extracts of Indigofera hirsuta (Ih-E) and Afrormosia laxiflora (Al-E). The results showed that total phenolic and flavonoid contents in dry Ih-E were 54.38 mg and 116.03 mg/gram, respectively, while in AlE were 170.46 mg and 80.23 mg/gram. Both extracts exhibited antioxidant activities since they decreased lipid peroxidation, but increased antioxidant activities and anti-inflammatory activities as shown from inhibition of RBC haemolysis and inhibition of albumin denaturation. Also, they have cytotoxic effect against normal BHK-21 and HepG2 where IC 50 for Ih-E were 309 µg/ml and 95µg/ml, respectively (selectivity index 1.58), while for AlE 57.50µg/ml and 144.50 µg/ml, respectively (selectivity index 0.40). Ih-E has antimicrobial activities towards Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio species and E-coli, while AlE showed no antimicrobial activities. Conclusion: Both extracts possessed antioxidant, anti-inflammatory and anticancer activities, thus, can be used as pharmacological tools.
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](Background: Oxidative stress (OS) and inflammation are the central pathogenic events in liver dis... more Background: Oxidative stress (OS) and inflammation are the central pathogenic events in liver diseases. In this study, the protective and therapeutic role of Carica Papaya Linn. seeds extract (SE) was evaluated against the hepatotoxicity induced by carbon tetrachloride (CCl 4) in rats. Methods: The air-dried papaya seeds were powdered and extracted with distilled water. The phytochemical ingredients, minerals, and antioxidant potentials were studied. For determination of the biological role of SE against hepatotoxicity induced by CCl 4 , five groups of adult male Sprague-Dawley rats were prepared (8 rats per each): C: control; SE: rats were administered with SE alone; CCl 4 : rats were injected subcutaneously with CCl 4 ; SE-CCl 4 group: rats were administered with SE orally for 2 weeks before and 8 weeks during CCl 4 injection; SE-CCl 4-SE group: Rats were administered with SE and CCl 4 as mentioned in SE-CCl 4 group with a prolonged administration with SE for 4 weeks after the stopping of CCl 4 injection. Then, the markers of OS [lipid peroxidation (LP) and antioxidant parameters; glutathione (GSH), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GPx)], inflammation [nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α, interleukin (IL)-6], fibrosis [transforming growth factor (TGF)-β], apoptosis [tumor suppressor gene (p53)], liver and kidney functions beside liver histopathology were determined. Results: The phytochemical analyses revealed that SE contains different concentrations of phenolics, flavonoids, terpenoids, and minerals so it has potent antioxidant activities. Therefore, the treatment with SE pre, during, and/or after CCl 4 administration attenuated the OS induced by CCl 4 where the LP was reduced, but the antioxidants (GSH, SOD, GST, and GPx) were increased. Additionally, these treatments reduced the inflammation, fibrosis, and apoptosis induced by CCl 4 , since the levels of NF-κB, TNF-α, IL-6, TGF-β, and p53 were declined. Accordingly, liver and kidney functions were improved. These results were confirmed by the histopathological results. Conclusions: SE has protective and treatment roles against hepatotoxicity caused by CCl 4 administration through the reduction of OS, inflammation, fibrosis, and apoptosis induced by CCl 4 and its metabolites in the liver tissues. Administration of SE for healthy rats for 12 weeks had no adverse effects. Thus, SE can be utilized in pharmacological tools as anti-hepatotoxicity.
Biochemistry Letters
Background: Ginger, the rhizome of Zingiber officinale, a commom constituent of diet worldwide, h... more Background: Ginger, the rhizome of Zingiber officinale, a commom constituent of diet worldwide, has some pharmacological activities. Objectives: The present study was undertaken to investigate the antiinflammatory effect of ginger extract against carrageenan-induced inflammation in the rat paw. Methods: One kilogram of air-dried rhizomes of zingiber officinale was extracted with 6L ethyl alcohol (96%). Thirty-six male Wister rats were divided into 6 groups (6 per each); the control group (C-Group): rats were injected intraperitoneally by DMSO (0.1 ml/kg). Ca-Group: rats were injected intraperitoneally with DMSO followed by subcutaneous injection with carrageenan solution [0.1 ml (1% w/v)]. Ce + Ca Group: rats were injected intraperitoneally with single dose of CeleCOXib (25 mg/kg) as NSAID. After that, it were injected subcutaneously by carrageenan solution [0.1 ml (1% w/v)]. G 300 +Ca Group: rats were injected intraperitoneally by ethanolic ginger extract (300 mg/kg) followed by a subcutaneous injection with carrageenan solution. G 600 +Ca Group: rats were injected intraperitoneally with ethanolic ginger extract (600 mg/kg) followed by a subcutaneous injection with carrageenan solution. G 900 +Ca Group: rats were injected intraperitoneally with ethanolic ginger extract (900 mg/kg) followed by a subcutaneous injection with carrageenan solution. Results: Carrageenan treatment induced significant increases in the activities of COX-2 and LOX as well as the MDA level while caused significant decline in GSH level compared to the control group. The rats injected with ginger extract followed by carrageenan showed significant decline in the activities of COX-2 and LOX as well as the MDA level compared to the carrageenan-treated group. Our results indicated that the ginger at the higher doses were more effective. Our results concluded that the ginger extract causes inhibition of the edema in paw especially at the higher doses compared to the reference drug.
Response letter It is with excitement that I resubmit to you again a revised version of manuscrip... more Response letter It is with excitement that I resubmit to you again a revised version of manuscript BCAM-D-1900193 " The synergistic hepatoprotective potential of Beta vulgaris juice and 2,3dimercaptosuccinic acid in lead-intoxicated rats via improving the hepatic oxidative and inflammatory stress ", BMC Complementary & Alternative Medicine. Authors thank again journal editor and reviewers for their efforts in getting the paper out in the best form. The manuscript has certainly benefited from these perceptive revision recommendations. Revisions in the text are shown with a green highlight. The following are our point-by-point responses to each of the reviewers' comments. All line numbers here refer to the revised manuscript.
Background: Altered microRNAs (miRNAs) expression has an impact on cancer initiation and progress... more Background: Altered microRNAs (miRNAs) expression has an impact on cancer initiation and progression. There is an emerging evidence that circulating miR21 and miR34a may have a potential role in breast cancer (BC) diagnosis and prognosis. Aim of the work: The current study aimed at characterizing the expression pattern of miR21 and miR34a in BC female patients preand post chemotherapy and delineating their correlation with clinicopathological subtypes and other molecular biomarkers. Patients and Methods: Real time quantitative polymerase chain reaction (RQ PCR) was used to assess the relative expression of miR21 and miR34a in sera of 179 BC female patients in relation to 58 healthy females. Results: Circulating miR21 and miR34a showed significant upregulation of 5.1 fold change (p<0.001) and downregulation of -5.63 fold change, respectively (p<0.001). Data showed higher levels of miR21 in triple negative (TN), basal like subtype and stage III BC patients (p<0.001). Higher m...
Persistent hepatitis B virus (HBV) infection often leads to the development of chronic hepatitis,... more Persistent hepatitis B virus (HBV) infection often leads to the development of chronic hepatitis, cirrhosis and hepatocellular carcinoma. The aim of this study was to estimate the oxidant/antioxidant status and liver function tests in different stages of HBV infection to investigate the role of oxidative stress in the pathogenesis of chronic liver diseases. The study was carried out on 150 control healthy persons (C) and 320 patients with HBV infection which included normal carriers (N), acute (A), chronic (CH), cirrhosis (Cl) and hepatocellular carcinoma (HCC). The study focused on the determination of malondialdehyde (MDA), non enzymatic and enzymatic antioxidant parameters and liver function profile tests. The results showed that MDA and glutathione reductase (GSR) were increased, while reduced glutathione (GSH), total glutathione s-transferase (GST), total glutathione peroxidase (GPx), superoxide dismutase (SOD) were decreased in N, A, CH, CI and HCC groups as compared to the C ...
The kidney possesses most of the common xenobiotic metabolizing enzymes, and is thus able to make... more The kidney possesses most of the common xenobiotic metabolizing enzymes, and is thus able to make an important contribution to the body's metabolism of drugs and foreign compounds. The effect of pyrimidine derivatives 6-amino-2-thiouracil (ATU), 2-thiouracil (TU) and 5-flurouracil (5FU), and pomegranate juice (PJ) on kidney nitric oxide (NO), malondialdehyde (MDA), DNA fragmentation (DNAF), caspase-3 levels and kidney function tests in rats treated with diethyl nitrosamine (DEN) and carbon tetra chloride CCl4 was studied. The effect of PJ on rat not treated with DEN and CCl4 was studied also. Administration of rats with DEN and CCl4 caused an elevation in the levels of NO, MDA, DNAF, caspase-3 and kidney function tests, compared to the control. Treatment of rats with PJ pre, during, and post DEN and CCl4 administration improved kidney function and decreased the levels of NO, MDA, DNAF, and caspase-3 activities better than that in DEN-5FU, DENATU, DEN-TU groups compared to the DE...
The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-... more The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3- carboxamide-1,1-dioxide.) on lipoxygenase enzyme (LOX) in different tissues of rabbits in vitro and in vivo were studied. MLX (117 nM) inhibited trachea, skin, stomach and serum LOX enzyme activities in vitro with varying inhibitory degrees. The administration of MLX (0.214 mg/kg body mass/day for 7days) inhibited trachea, skin and stomach LOX activities. However, MLX had no effect on serum LOX activity. Linweaver-Burk plots showed that MLX inhibited LOX enzyme in vitro and in vivo by competitive inhibition manner. The values of Km and Vmax in the absence and presence of MLX were determined. (Nadia Z. Shabanand Ahmed A. Abdul-Aziz. Effect of Meloxicam on Lipoxygenase Activity in Trachea, Skin, Stomach and Serum of Rabbits (in Vivo and in Vitro). Life Sci J 2014;11(6):48-55). (ISSN:1097-8135). http://www.lifesciencesite.com . 7
Pharmacy & Pharmacology International Journal, Apr 13, 2018
Agar wood has been applied to traditional medicine and used as perfumery and food ingredients esp... more Agar wood has been applied to traditional medicine and used as perfumery and food ingredients especially in Saudi Arabia. It contains bio molecules component that shows various pharmacological, anti-mutagenic, anti-tumor and anti-helminthic, antioxidant and other advantages effects. Reactive oxygen species (ROS) produced during the detoxification of xenobiotics and drugs that generated oxidative stress and apoptosis. Caspase-3 is the central executioners, a cysteine protease, during apoptosis and cell death. The effect of Agar wood hexane extract on liver death through studied of nitric oxide (NO), malondialdehyde (MDA), caspase-3 levels and liver function tests in rats treat and untreated with an intra peritoneal dose of 3g/kg methanol was studied in this investigation. Injected of rats with methanol-induced an elevation in the levels of NO, MDA, caspase-3, and liver function tests, compared to the control. Treatment of animals with Agar wood extracts pre, during, and post methanol administration improved liver function and decreased the levels of NO, MDA, and caspase-3 activities compared to the methanol group, indicates that Agar wood diminished the oxidative stress and apoptosis induced by methanol, this may be due to its Phytochemical compositions.
Vaccine Research, 2017
Introduction: Dendritic cells (DCs) have been recently employed as carriers for vaccines against ... more Introduction: Dendritic cells (DCs) have been recently employed as carriers for vaccines against several viral infections. The present study was designed to develop a prophylactic vaccine against hepatitis C virus (HCV) using DCs treated with Berberis vulgaris root extract (BRE), as a preclinical study. Methods: BRE was prepared and injected to female BALB/c mice for DCs expansion. The expanded splenocyte cells (EDC) were purified and efficiently loaded ex vivo with HCV-nonstructural protein 3, NS3, (EDC-NS3). Mice were subcutaneously inoculated with EDC-NS3 vaccine candidate thrice with 4-week-intervals and IL-12, IFN-γ, IL-4, IL-10, MHC class II, CD3, CD16, indoleamine 2, 3 dioxygenase (IDO) and total protein levels were measured, post-vaccination by PCR and flow cytometry. Moreover, cytotoxic T lymphocyte and humoral immune responses were examined. Results: Our data revealed that immunization with EDC-NS3 vaccine elevated IL-12, IFN-γ and IL-4 expressions as well as MHC II and CD...
Hydrobiologia, 1984
Urea is an excellent nitrogen source for the growth of many algae (Syrett 1962; Naylor 1970). In ... more Urea is an excellent nitrogen source for the growth of many algae (Syrett 1962; Naylor 1970). In algae, the enzymes which degrade urea are urease and ATP: urea amidolyase (U ALase) (Leftley & Syrett 1973). The properties of urease and U ALase are obviously different; consequently, they can be easily distinguished in extracts. U ALase requires ATP, Mg2+, K+ and is inhibited by avidin (Roon & Levenberg, 1968; Rognes et al. 1969). The purified enzyme has an absolute requirement for CO2 (Roon & Levenberg 1968). Urease does not require cations and is not stimulated by ATP or inhibited by avidin.
Scientific Reports
Lead (Pb) toxicity is one of the most prevalent causes of human neurotoxicity. The available chel... more Lead (Pb) toxicity is one of the most prevalent causes of human neurotoxicity. The available chelator drugs used now have many adverse effects. So, in this study, the protective role of Betavulgaris juice (BVJ) on rat neurotoxicity induced by Pb was evaluated and the results were compared with the results of dimercaptosuccinic acid (DMSA, as used drug). Additionally, the synergistic effect of BVJ and DMSA against Pb-induced neurotoxicity was assessed. The study focused on the determination of the antioxidant, anti-inflammatory, and neurological potential of BVJ (alone, and with DMSA) towards lead-induced neurotoxicity. Also, the characterization of BVJ was studied. The results showed that BVJ contains considerable quantities of polyphenols, triterpenoids, and betalains which play an important role as antioxidants and anti-inflammatory. BVJ exhibited a protective effect against neurotoxicity via the reduction of Pb levels in blood and brain. Moreover, BVJ decreased the oxidative stre...
BMC Complementary Medicine and Therapies
Background Lead (Pb) is observed in all areas of the environment, mainly derived from human opera... more Background Lead (Pb) is observed in all areas of the environment, mainly derived from human operations such as mining, processing, and burning fossil fuels. Pb toxicity is one of the most prevalent causes of human hepatotoxicity. The available chelator drugs used now have many adverse effects and therefore the world is looking for natural and secure alternatives. Methods Here, we evaluated the hepatoprotective role of the oral administration (1 g/kg b.w.) of the lyophilized Beta vulgaris juice (BVJ) against Pb-induced rat hepatotoxicity. We also examined the possible synergistic hepatoprotective impact of the combination between BVJ and 2,3- dimercaptosuccinic acid (DMSA, the currently approved drug for Pb-toxicity). The evaluation depends on the ability of BVJ, DMSA, or their combination (BVJ-DMSA) to reduce serum and hepatic Pb level and to avoid oxidative stress and inflammation caused by Pb. The level of lipid peroxidation, reduced glutathione (GSH), total antioxidant capacity, ...
Environmental Science and Pollution Research
The present study was done to evaluate the protective and therapeutic role of mango pulp (M), epr... more The present study was done to evaluate the protective and therapeutic role of mango pulp (M), eprosartan drug (E), and their co-administration (EM) against hepatotoxicity induced by thioacetamide (T). Seven groups of rats were prepared as follows: the control (C) group (normal rats), T group (the rats were injected with T), T-M group (the rats were injected with T, and then treated with M), T-E group (the rats were injected with T, and then treated with E), T-EM group (the rats were injected with T, and then treated with E and M), M-TM-M group (the rats were administered with M before, during, and after T injection), and M group (the healthy rats were administered with M only). Firstly, the characterizations of M were determined. Also, the markers of hepatic oxidative stress [malondialdehyde (MDA) and glutathione (GSH) levels and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GSR)], inflammation and fibrosis [(tumor necrosis fa...
Many manuscripts and historical books contain a form of deterioration known as foxing or fox spot... more Many manuscripts and historical books contain a form of deterioration known as foxing or fox spots, a brownish stain which has the effect of altering aesthetic and visual appeal. The aim of this work is to study the role of the extracts of Water Cantaloupe (CE) and Water melon (WE) separately as natural products in removing foxing spots in various modern and old papers. Old papers and three types of modern papers made from cotton, linen and a mixture of cotton, linen and wood (1:1:1) were used for this purpose. Each type was divided into two groups, one of them was infected with foxing and the other was left as control (uninfected). Infected papers were treated with CE, WE and 2% sodium hypochlorite (as a traditional chemical bleaching) separately. Fourier Transform Infrared Spectroscopy (FTIR), Atomic Absorption Spectroscopy, Scanning Electron Microscopy equipped with Energy-Dispersive X-ray spectroscopy (SEM-EDX) in addition to some optical and mechanical properties were carried o...
Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In... more Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In our previous studies, dithiophenolato titanium (IV) Complex "DBT" and its chitosan nanocomposite "DBT-CSNPs" were prepared and we showed that these compounds have antibacterial activities, cytotoxic, and have abilities to bind with DNA helixes. Therefore, in this study, we evaluated the LD50 values of dithiophenolato titanium (IV)-complex (DBT) and its high thermal stable chitosan nanoparticles (DBT-CSNPs). Then their therapeutic effects against liver injuries induced by carbon tetrachloride (CCl4) were assessed and compared with cisplatin treatment. Additionally, the anti-proliferative activity of DBT and DBT-CSNPs against human liver cancer (HepG2) cell lines through the analysis of the cell cycle was evaluated. Methods Nine groups of rats were prepared: normal, DBT, DBT-CSNPs, CSNPs, CCl4, CCl4-DBT, CCl4-DBT-CSNPs, CCl4-CSNPs and CCl4-cisplatin. Liver histopatholo...
Asian Pacific Journal of Cancer Prevention, 2015
The effect of Eruca sativa seed extract (SE) on nuclear factor kappa B (NF-κB), cyclooxygenase-2 ... more The effect of Eruca sativa seed extract (SE) on nuclear factor kappa B (NF-κB), cyclooxygenase-2 (COX-2) and B-cell lymphoma-2 (Bcl-2) gene expression levels was investigated in rat mammary gland carcinogenesis induced by 7,12 dimethylbenz(α)anthracene (DMBA). DMBA increased NF-κB, COX-2 and Bcl-2 gene expression levels and lipid peroxidation (LP), while, decreased glutathione-S-transferase (GST) and superoxide dismutase (SOD) activities and total antioxidant concentration (TAC) compared to the control group. After DMBA administration, SE treatment reduced NF-κB, COX-2 and Bcl-2 gene expression levels and LP. Hence, SE treatment reduced inflammation and cell proliferation, while increasing apoptosis, GST and SOD activities and TAC. Analysis revealed that SE has high concentrations of total flavonoids, triterpenoids, alkaloids and polyphenolic compounds such as gallic, chlorogenic, caffeic, 3,4-dicaffeoyl quinic, 3,5-dicaffeoyl quinic, tannic, cinnamic acids, catechin and phloridzin....
Abstract: The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzot... more Abstract: The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-carboxamide-1,1-dioxide.) on lipoxygenase enzyme (LOX) in different tissues of rabbits in vitro and in vivo were studied. MLX (117 nM) inhibited trachea, skin, stomach and serum LOX enzyme activities in vitro with varying inhibitory degrees. The administration of MLX (0.214 mg/kg body mass/day for 7days) inhibited trachea, skin and stomach LOX activities. However, MLX had no effect on serum LOX activity. Linweaver-Burk plots showed that MLX inhibited LOX enzyme in vitro and in vivo by competitive inhibition manner. The values of Km and Vmax in the absence and presence of MLX were determined.
Saudi Journal of Medical and Pharmaceutical Sciences
This study highlights the characterization and biological activities (in vitro) of root extracts ... more This study highlights the characterization and biological activities (in vitro) of root extracts of Indigofera hirsuta (Ih-E) and Afrormosia laxiflora (Al-E). The results showed that total phenolic and flavonoid contents in dry Ih-E were 54.38 mg and 116.03 mg/gram, respectively, while in AlE were 170.46 mg and 80.23 mg/gram. Both extracts exhibited antioxidant activities since they decreased lipid peroxidation, but increased antioxidant activities and anti-inflammatory activities as shown from inhibition of RBC haemolysis and inhibition of albumin denaturation. Also, they have cytotoxic effect against normal BHK-21 and HepG2 where IC 50 for Ih-E were 309 µg/ml and 95µg/ml, respectively (selectivity index 1.58), while for AlE 57.50µg/ml and 144.50 µg/ml, respectively (selectivity index 0.40). Ih-E has antimicrobial activities towards Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio species and E-coli, while AlE showed no antimicrobial activities. Conclusion: Both extracts possessed antioxidant, anti-inflammatory and anticancer activities, thus, can be used as pharmacological tools.
Background: Oxidative stress (OS) and inflammation are the central pathogenic events in liver dis... more Background: Oxidative stress (OS) and inflammation are the central pathogenic events in liver diseases. In this study, the protective and therapeutic role of Carica Papaya Linn. seeds extract (SE) was evaluated against the hepatotoxicity induced by carbon tetrachloride (CCl 4) in rats. Methods: The air-dried papaya seeds were powdered and extracted with distilled water. The phytochemical ingredients, minerals, and antioxidant potentials were studied. For determination of the biological role of SE against hepatotoxicity induced by CCl 4 , five groups of adult male Sprague-Dawley rats were prepared (8 rats per each): C: control; SE: rats were administered with SE alone; CCl 4 : rats were injected subcutaneously with CCl 4 ; SE-CCl 4 group: rats were administered with SE orally for 2 weeks before and 8 weeks during CCl 4 injection; SE-CCl 4-SE group: Rats were administered with SE and CCl 4 as mentioned in SE-CCl 4 group with a prolonged administration with SE for 4 weeks after the stopping of CCl 4 injection. Then, the markers of OS [lipid peroxidation (LP) and antioxidant parameters; glutathione (GSH), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GPx)], inflammation [nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α, interleukin (IL)-6], fibrosis [transforming growth factor (TGF)-β], apoptosis [tumor suppressor gene (p53)], liver and kidney functions beside liver histopathology were determined. Results: The phytochemical analyses revealed that SE contains different concentrations of phenolics, flavonoids, terpenoids, and minerals so it has potent antioxidant activities. Therefore, the treatment with SE pre, during, and/or after CCl 4 administration attenuated the OS induced by CCl 4 where the LP was reduced, but the antioxidants (GSH, SOD, GST, and GPx) were increased. Additionally, these treatments reduced the inflammation, fibrosis, and apoptosis induced by CCl 4 , since the levels of NF-κB, TNF-α, IL-6, TGF-β, and p53 were declined. Accordingly, liver and kidney functions were improved. These results were confirmed by the histopathological results. Conclusions: SE has protective and treatment roles against hepatotoxicity caused by CCl 4 administration through the reduction of OS, inflammation, fibrosis, and apoptosis induced by CCl 4 and its metabolites in the liver tissues. Administration of SE for healthy rats for 12 weeks had no adverse effects. Thus, SE can be utilized in pharmacological tools as anti-hepatotoxicity.
Biochemistry Letters
Background: Ginger, the rhizome of Zingiber officinale, a commom constituent of diet worldwide, h... more Background: Ginger, the rhizome of Zingiber officinale, a commom constituent of diet worldwide, has some pharmacological activities. Objectives: The present study was undertaken to investigate the antiinflammatory effect of ginger extract against carrageenan-induced inflammation in the rat paw. Methods: One kilogram of air-dried rhizomes of zingiber officinale was extracted with 6L ethyl alcohol (96%). Thirty-six male Wister rats were divided into 6 groups (6 per each); the control group (C-Group): rats were injected intraperitoneally by DMSO (0.1 ml/kg). Ca-Group: rats were injected intraperitoneally with DMSO followed by subcutaneous injection with carrageenan solution [0.1 ml (1% w/v)]. Ce + Ca Group: rats were injected intraperitoneally with single dose of CeleCOXib (25 mg/kg) as NSAID. After that, it were injected subcutaneously by carrageenan solution [0.1 ml (1% w/v)]. G 300 +Ca Group: rats were injected intraperitoneally by ethanolic ginger extract (300 mg/kg) followed by a subcutaneous injection with carrageenan solution. G 600 +Ca Group: rats were injected intraperitoneally with ethanolic ginger extract (600 mg/kg) followed by a subcutaneous injection with carrageenan solution. G 900 +Ca Group: rats were injected intraperitoneally with ethanolic ginger extract (900 mg/kg) followed by a subcutaneous injection with carrageenan solution. Results: Carrageenan treatment induced significant increases in the activities of COX-2 and LOX as well as the MDA level while caused significant decline in GSH level compared to the control group. The rats injected with ginger extract followed by carrageenan showed significant decline in the activities of COX-2 and LOX as well as the MDA level compared to the carrageenan-treated group. Our results indicated that the ginger at the higher doses were more effective. Our results concluded that the ginger extract causes inhibition of the edema in paw especially at the higher doses compared to the reference drug.
Response letter It is with excitement that I resubmit to you again a revised version of manuscrip... more Response letter It is with excitement that I resubmit to you again a revised version of manuscript BCAM-D-1900193 " The synergistic hepatoprotective potential of Beta vulgaris juice and 2,3dimercaptosuccinic acid in lead-intoxicated rats via improving the hepatic oxidative and inflammatory stress ", BMC Complementary & Alternative Medicine. Authors thank again journal editor and reviewers for their efforts in getting the paper out in the best form. The manuscript has certainly benefited from these perceptive revision recommendations. Revisions in the text are shown with a green highlight. The following are our point-by-point responses to each of the reviewers' comments. All line numbers here refer to the revised manuscript.
Background: Altered microRNAs (miRNAs) expression has an impact on cancer initiation and progress... more Background: Altered microRNAs (miRNAs) expression has an impact on cancer initiation and progression. There is an emerging evidence that circulating miR21 and miR34a may have a potential role in breast cancer (BC) diagnosis and prognosis. Aim of the work: The current study aimed at characterizing the expression pattern of miR21 and miR34a in BC female patients preand post chemotherapy and delineating their correlation with clinicopathological subtypes and other molecular biomarkers. Patients and Methods: Real time quantitative polymerase chain reaction (RQ PCR) was used to assess the relative expression of miR21 and miR34a in sera of 179 BC female patients in relation to 58 healthy females. Results: Circulating miR21 and miR34a showed significant upregulation of 5.1 fold change (p<0.001) and downregulation of -5.63 fold change, respectively (p<0.001). Data showed higher levels of miR21 in triple negative (TN), basal like subtype and stage III BC patients (p<0.001). Higher m...
Persistent hepatitis B virus (HBV) infection often leads to the development of chronic hepatitis,... more Persistent hepatitis B virus (HBV) infection often leads to the development of chronic hepatitis, cirrhosis and hepatocellular carcinoma. The aim of this study was to estimate the oxidant/antioxidant status and liver function tests in different stages of HBV infection to investigate the role of oxidative stress in the pathogenesis of chronic liver diseases. The study was carried out on 150 control healthy persons (C) and 320 patients with HBV infection which included normal carriers (N), acute (A), chronic (CH), cirrhosis (Cl) and hepatocellular carcinoma (HCC). The study focused on the determination of malondialdehyde (MDA), non enzymatic and enzymatic antioxidant parameters and liver function profile tests. The results showed that MDA and glutathione reductase (GSR) were increased, while reduced glutathione (GSH), total glutathione s-transferase (GST), total glutathione peroxidase (GPx), superoxide dismutase (SOD) were decreased in N, A, CH, CI and HCC groups as compared to the C ...
The kidney possesses most of the common xenobiotic metabolizing enzymes, and is thus able to make... more The kidney possesses most of the common xenobiotic metabolizing enzymes, and is thus able to make an important contribution to the body's metabolism of drugs and foreign compounds. The effect of pyrimidine derivatives 6-amino-2-thiouracil (ATU), 2-thiouracil (TU) and 5-flurouracil (5FU), and pomegranate juice (PJ) on kidney nitric oxide (NO), malondialdehyde (MDA), DNA fragmentation (DNAF), caspase-3 levels and kidney function tests in rats treated with diethyl nitrosamine (DEN) and carbon tetra chloride CCl4 was studied. The effect of PJ on rat not treated with DEN and CCl4 was studied also. Administration of rats with DEN and CCl4 caused an elevation in the levels of NO, MDA, DNAF, caspase-3 and kidney function tests, compared to the control. Treatment of rats with PJ pre, during, and post DEN and CCl4 administration improved kidney function and decreased the levels of NO, MDA, DNAF, and caspase-3 activities better than that in DEN-5FU, DENATU, DEN-TU groups compared to the DE...
The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-... more The effect of meloxicam (MLX: 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3- carboxamide-1,1-dioxide.) on lipoxygenase enzyme (LOX) in different tissues of rabbits in vitro and in vivo were studied. MLX (117 nM) inhibited trachea, skin, stomach and serum LOX enzyme activities in vitro with varying inhibitory degrees. The administration of MLX (0.214 mg/kg body mass/day for 7days) inhibited trachea, skin and stomach LOX activities. However, MLX had no effect on serum LOX activity. Linweaver-Burk plots showed that MLX inhibited LOX enzyme in vitro and in vivo by competitive inhibition manner. The values of Km and Vmax in the absence and presence of MLX were determined. (Nadia Z. Shabanand Ahmed A. Abdul-Aziz. Effect of Meloxicam on Lipoxygenase Activity in Trachea, Skin, Stomach and Serum of Rabbits (in Vivo and in Vitro). Life Sci J 2014;11(6):48-55). (ISSN:1097-8135). http://www.lifesciencesite.com . 7
Pharmacy & Pharmacology International Journal, Apr 13, 2018
Agar wood has been applied to traditional medicine and used as perfumery and food ingredients esp... more Agar wood has been applied to traditional medicine and used as perfumery and food ingredients especially in Saudi Arabia. It contains bio molecules component that shows various pharmacological, anti-mutagenic, anti-tumor and anti-helminthic, antioxidant and other advantages effects. Reactive oxygen species (ROS) produced during the detoxification of xenobiotics and drugs that generated oxidative stress and apoptosis. Caspase-3 is the central executioners, a cysteine protease, during apoptosis and cell death. The effect of Agar wood hexane extract on liver death through studied of nitric oxide (NO), malondialdehyde (MDA), caspase-3 levels and liver function tests in rats treat and untreated with an intra peritoneal dose of 3g/kg methanol was studied in this investigation. Injected of rats with methanol-induced an elevation in the levels of NO, MDA, caspase-3, and liver function tests, compared to the control. Treatment of animals with Agar wood extracts pre, during, and post methanol administration improved liver function and decreased the levels of NO, MDA, and caspase-3 activities compared to the methanol group, indicates that Agar wood diminished the oxidative stress and apoptosis induced by methanol, this may be due to its Phytochemical compositions.
Vaccine Research, 2017
Introduction: Dendritic cells (DCs) have been recently employed as carriers for vaccines against ... more Introduction: Dendritic cells (DCs) have been recently employed as carriers for vaccines against several viral infections. The present study was designed to develop a prophylactic vaccine against hepatitis C virus (HCV) using DCs treated with Berberis vulgaris root extract (BRE), as a preclinical study. Methods: BRE was prepared and injected to female BALB/c mice for DCs expansion. The expanded splenocyte cells (EDC) were purified and efficiently loaded ex vivo with HCV-nonstructural protein 3, NS3, (EDC-NS3). Mice were subcutaneously inoculated with EDC-NS3 vaccine candidate thrice with 4-week-intervals and IL-12, IFN-γ, IL-4, IL-10, MHC class II, CD3, CD16, indoleamine 2, 3 dioxygenase (IDO) and total protein levels were measured, post-vaccination by PCR and flow cytometry. Moreover, cytotoxic T lymphocyte and humoral immune responses were examined. Results: Our data revealed that immunization with EDC-NS3 vaccine elevated IL-12, IFN-γ and IL-4 expressions as well as MHC II and CD...
Hydrobiologia, 1984
Urea is an excellent nitrogen source for the growth of many algae (Syrett 1962; Naylor 1970). In ... more Urea is an excellent nitrogen source for the growth of many algae (Syrett 1962; Naylor 1970). In algae, the enzymes which degrade urea are urease and ATP: urea amidolyase (U ALase) (Leftley & Syrett 1973). The properties of urease and U ALase are obviously different; consequently, they can be easily distinguished in extracts. U ALase requires ATP, Mg2+, K+ and is inhibited by avidin (Roon & Levenberg, 1968; Rognes et al. 1969). The purified enzyme has an absolute requirement for CO2 (Roon & Levenberg 1968). Urease does not require cations and is not stimulated by ATP or inhibited by avidin.
Scientific Reports
Lead (Pb) toxicity is one of the most prevalent causes of human neurotoxicity. The available chel... more Lead (Pb) toxicity is one of the most prevalent causes of human neurotoxicity. The available chelator drugs used now have many adverse effects. So, in this study, the protective role of Betavulgaris juice (BVJ) on rat neurotoxicity induced by Pb was evaluated and the results were compared with the results of dimercaptosuccinic acid (DMSA, as used drug). Additionally, the synergistic effect of BVJ and DMSA against Pb-induced neurotoxicity was assessed. The study focused on the determination of the antioxidant, anti-inflammatory, and neurological potential of BVJ (alone, and with DMSA) towards lead-induced neurotoxicity. Also, the characterization of BVJ was studied. The results showed that BVJ contains considerable quantities of polyphenols, triterpenoids, and betalains which play an important role as antioxidants and anti-inflammatory. BVJ exhibited a protective effect against neurotoxicity via the reduction of Pb levels in blood and brain. Moreover, BVJ decreased the oxidative stre...
BMC Complementary Medicine and Therapies
Background Lead (Pb) is observed in all areas of the environment, mainly derived from human opera... more Background Lead (Pb) is observed in all areas of the environment, mainly derived from human operations such as mining, processing, and burning fossil fuels. Pb toxicity is one of the most prevalent causes of human hepatotoxicity. The available chelator drugs used now have many adverse effects and therefore the world is looking for natural and secure alternatives. Methods Here, we evaluated the hepatoprotective role of the oral administration (1 g/kg b.w.) of the lyophilized Beta vulgaris juice (BVJ) against Pb-induced rat hepatotoxicity. We also examined the possible synergistic hepatoprotective impact of the combination between BVJ and 2,3- dimercaptosuccinic acid (DMSA, the currently approved drug for Pb-toxicity). The evaluation depends on the ability of BVJ, DMSA, or their combination (BVJ-DMSA) to reduce serum and hepatic Pb level and to avoid oxidative stress and inflammation caused by Pb. The level of lipid peroxidation, reduced glutathione (GSH), total antioxidant capacity, ...