Nadya Galeva - Academia.edu (original) (raw)

Papers by Nadya Galeva

Research paper thumbnail of Two-dimensional separation of the membrane protein sarcoplasmic reticulum Ca–ATPase for high-performance liquid chromatography–tandem mass spectrometry analysis of posttranslational protein modifications

Analytical Biochemistry, 2002

For the characterization of posttranslational modifications of the sarcoplasmic/endoplasmic retic... more For the characterization of posttranslational modifications of the sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA), we developed a two-dimensional separation protocol based on reversed-phase HPLC followed by SDS-PAGE and LC-MS/MS analysis of in-gel tryptic digests. Representative experiments are shown for the rabbit fast-twitch skeletal muscle isoform SERCA1. Matrixassisted laser desorption-ionization and electrospray ionization-mass spectrometry analyses of SERCA1 tryptic digests revealed ca. 66% coverage of the protein sequence. This approach was used for the detection and quantitation of nitrotyrosine formation after exposure of SERCA1 to peroxynitrite in vitro. At molar ratios of nitrotyrosine to protein of 0.23 we confirmed by LC-MS/MS the nitration of predominantly Tyr 122 in the SERCA1 sequence. Ó

Research paper thumbnail of Synthesis of C60F48 in the reaction of C60 with molecular fluorine

Research paper thumbnail of Reactions of Fullerenes with Difluorine

Russian Journal of Inorganic Chemistry

ABSTRACT

Research paper thumbnail of Structure of C60F48 by solid state MAS19F, 13C NMR

Research paper thumbnail of Cytochrome P450 Isozymes Proteomic Approach to the Profiling of Cytochrome P450 Isozymes

Research paper thumbnail of The low temperature heat capacities of fluorofullerenes

The Journal of Chemical Thermodynamics, 1999

ABSTRACT

Research paper thumbnail of ChemInform Abstract: A Mass Spectrometric Study of C60F48

ChemInform, 1998

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of ChemInform Abstract: Reactions of Fullerenes with Molecular Fluorine

ChemInform, 2000

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of ChemInform Abstract: Direct Fluorination of Fullerenes

ChemInform, 2000

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of SUMOylation and Ubiquitylation Circuitry Controls Pregnane X Receptor Biology in Hepatocytes

Drug metabolism and disposition: the biological fate of chemicals, Jan 10, 2015

Several nuclear receptor (NR) superfamily members are known to be the molecular target of either ... more Several nuclear receptor (NR) superfamily members are known to be the molecular target of either the SUMO- or ubiquitin-signaling pathways. However, little is currently known regarding how these two post-translational modifications interact to control NR biology. We show that the SUMO and ubiquitin circuitry coordinately modifies pregnane x receptor (PXR, NR1I2) to play a key role in regulating PXR protein stability, transactivation capacity, and transcriptional repression. The SUMOylation and ubiquitylation of PXR is increased in a ligand- and tumor necrosis factor-alpha (TNFα)-dependent manner in hepatocytes. The SUMO-E3 ligase enzymes PIAS1 and PIASy drive high levels of PXR SUMOylation. Expression of PIAS1 selectively increases SUMO(3)ylation, as well as PXR-mediated induction of CYP3A and the xenobiotic response. The PIASy-mediated SUMO(1)ylation imparts a transcriptionally repressive function by ameliorating interaction of PXR with co-activator protein peroxisome proliferator-...

Research paper thumbnail of bi047367rsi20050124 043140

Research paper thumbnail of Venom variation and chemoreception of the viperid Agkistrodon contortrix: evidence for adaptation?

Journal of chemical ecology, 2003

Previous studies of chemoreceptive behavior in vipers suggest that snakes focus on the scent of e... more Previous studies of chemoreceptive behavior in vipers suggest that snakes focus on the scent of envenomated tissue to track their prey following envenomation. Other studies have indicated a correlation between qualitative differences in venom biochemistry and geographic variation in diet. The North American copperhead (Agkistrodon contortrix) varies geographically in diet and venom biochemistry; snakes were collected from three populations (Kansas, Texas, and Louisiana) that are known to have different prey preferences. Behavioral experiments were conducted to assess whether copperheads preferred envenomated prey more than nonenvenomated prey, as do other species of vipers studied thus far. Additional experiments tested the ability of copperheads to distinguish between envenomated prey from different geographic populations, and between geographic populations of copperheads and two other species of viper. Results indicated that copperheads prefer envenomated prey to nonenvenomated pr...

Research paper thumbnail of The host outer membrane proteins OmpA and OmpC are associated with the Shigella phage Sf6 virion

Virology, 2011

Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell c... more Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell components in phage assembly haven't been well understood. It was previously reported that two unidentified proteins were present in bacteriophage Sf6 virion (Casjens et al, 2004, J.Mol.Biol. 339, 379-394, Fig. 2A). Using tandem mass spectrometry, we have identified the two proteins as outer membrane proteins (OMPs) OmpA and OmpC from its host Shigella flexneri. The transmission electron cryo-microscopy structure of Sf6 shows significant density at specific sites at the phage capsid inner surface. This density fit well with the characteristic beta-barrel domains of OMPs, thus may be due to the two host proteins. Locations of this density suggest a role in Sf6 morphogenesis reminiscent of phage-encoded cementing proteins. These data indicate a new, OMP-related phage:host linkage, adding to previous knowledge that some lambdoid bacteriophage genomes contain OmpC-like genes that express phage-encoded porins in the lysogenic state.

Research paper thumbnail of The standard molar enthalpy of formation of fluorofullereneC60F48

The Journal of Chemical Thermodynamics, 1999

The standard molar enthalpy of combustion of fluorinated fullereneC60F48 was determined by rotati... more The standard molar enthalpy of combustion of fluorinated fullereneC60F48 was determined by rotating-bomb calorimetry,ΔcHmo=−(24638 ± 163)kJ · mol−1. Using this result and the standard molar enthalpy of sublimation,ΔsubHmo= (109 ± 7)kJ · mol−1, the values of the standard molar enthalpies of formation of C60F48in the crystalline and gaseous states were found to be −(7563 ± 166)kJ · mol−1and −(7454 ±

Research paper thumbnail of Saturated vapor pressure and sublimation enthalpy of C60F 18

The Journal of Chemical Thermodynamics, 2002

... Cited By in Scopus (8). Permissions & Reprints. Regular Article. Saturated vapor pressure... more ... Cited By in Scopus (8). Permissions & Reprints. Regular Article. Saturated vapor pressure and sublimation enthalpy of C 60 F 18. Guido Gigli, a , 1 , Giovanni Balducci, a Vitaliy Yu. ... References. 1. AA Gakh, AA Tuinman, JL Adcock and RN Compton. Tetrahedron Lett. 34 (1993), pp ...

Research paper thumbnail of Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria

Research paper thumbnail of Direct fluorination of fullerenes

Russian Chemical Reviews, 2000

... According to mass spectroscopic data, the main products, viz., white powders, were mixtures o... more ... According to mass spectroscopic data, the main products, viz., white powders, were mixtures of fluorofuller-enes C60F38 ± 46 (Fig. 2); however, no perfluorinated fullerene C60F60 was detected, the maximum degree of fluorination being 48 (the C60F48 molecule). ...

Research paper thumbnail of Direct Infusion Mass Spectrometry of Oxylipin-Containing Arabidopsis Membrane Lipids Reveals Varied Patterns in Different Stress Responses

PLANT PHYSIOLOGY, 2012

Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized f... more Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized fatty acyl anions revealed 86 mass spectral peaks representing polar membrane lipids in extracts from Arabidopsis (Arabidopsis thaliana) infected with Pseudomonas syringae pv tomato DC3000 expressing AvrRpt2 (PstAvr). Quadrupole time-of-flight and Fourier transform ion cyclotron resonance mass spectrometry provided evidence for the presence of membrane lipids containing one or more oxidized acyl chains. The membrane lipids included molecular species of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyldiacylglycerol, monogalactosyldiacylglycerol, and acylated monogalactosyldiacylglycerol. The oxidized chains were identified at the level of chemical formula and included C 18 H 27 O 3 (abbreviated 18:4-O, to indicate four double bond equivalents and one oxygen beyond the carbonyl group), C 18 H 29 O 3 (18:3-O), C 18 H 31 O 3 (18:2-O), C 18 H 29 O 4 (18:3-2O), C 18 H 31 O 4 (18:2-2O), and C 16 H 23 O 3 (16:4-O)

Research paper thumbnail of Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis

Neurobiology of Aging, 2010

Brain aging is associated with a progressive decline in cognitive function though the molecular m... more Brain aging is associated with a progressive decline in cognitive function though the molecular mechanisms remain unknown. Functional changes in brain neurons could be due to age-related alterations in levels of specific proteins critical for information processing. Specialized membrane microdomains known as 'lipid rafts' contain protein complexes involved in many signal transduction processes. This study was undertaken to determine if two-dimensional fluorescence difference gel electrophoresis (2D DIGE) analysis of proteins in synaptic membrane lipid rafts revealed agedependent alterations in levels of raft proteins. Five pairs of young and aged rat synaptic membrane rafts were subjected to DIGE separation, followed by image analysis and identification of significantly altered proteins. Of 1046 matched spots on DIGE gels, 94 showed statistically significant differences in levels between old and young rafts, and 87 of these were decreased in aged rafts. The 41 most significantly altered (p < 0.03) proteins included several synaptic proteins involved in energy metabolism, redox homeostasis, and cytoskeletal structure. This may indicate a disruption in bioenergetic balance and redox homeostasis in synaptic rafts with brain aging. Differential levels of representative identified proteins were confirmed by immunoblot analysis. Our findings provide novel pathways in investigations of mechanisms that may contribute to altered neuronal function in aging brain.

Research paper thumbnail of A mass spectrometric study of C60F48

Mendeleev Communications, 1997

ABSTRACT

Research paper thumbnail of Two-dimensional separation of the membrane protein sarcoplasmic reticulum Ca–ATPase for high-performance liquid chromatography–tandem mass spectrometry analysis of posttranslational protein modifications

Analytical Biochemistry, 2002

For the characterization of posttranslational modifications of the sarcoplasmic/endoplasmic retic... more For the characterization of posttranslational modifications of the sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA), we developed a two-dimensional separation protocol based on reversed-phase HPLC followed by SDS-PAGE and LC-MS/MS analysis of in-gel tryptic digests. Representative experiments are shown for the rabbit fast-twitch skeletal muscle isoform SERCA1. Matrixassisted laser desorption-ionization and electrospray ionization-mass spectrometry analyses of SERCA1 tryptic digests revealed ca. 66% coverage of the protein sequence. This approach was used for the detection and quantitation of nitrotyrosine formation after exposure of SERCA1 to peroxynitrite in vitro. At molar ratios of nitrotyrosine to protein of 0.23 we confirmed by LC-MS/MS the nitration of predominantly Tyr 122 in the SERCA1 sequence. Ó

Research paper thumbnail of Synthesis of C60F48 in the reaction of C60 with molecular fluorine

Research paper thumbnail of Reactions of Fullerenes with Difluorine

Russian Journal of Inorganic Chemistry

ABSTRACT

Research paper thumbnail of Structure of C60F48 by solid state MAS19F, 13C NMR

Research paper thumbnail of Cytochrome P450 Isozymes Proteomic Approach to the Profiling of Cytochrome P450 Isozymes

Research paper thumbnail of The low temperature heat capacities of fluorofullerenes

The Journal of Chemical Thermodynamics, 1999

ABSTRACT

Research paper thumbnail of ChemInform Abstract: A Mass Spectrometric Study of C60F48

ChemInform, 1998

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of ChemInform Abstract: Reactions of Fullerenes with Molecular Fluorine

ChemInform, 2000

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of ChemInform Abstract: Direct Fluorination of Fullerenes

ChemInform, 2000

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Research paper thumbnail of SUMOylation and Ubiquitylation Circuitry Controls Pregnane X Receptor Biology in Hepatocytes

Drug metabolism and disposition: the biological fate of chemicals, Jan 10, 2015

Several nuclear receptor (NR) superfamily members are known to be the molecular target of either ... more Several nuclear receptor (NR) superfamily members are known to be the molecular target of either the SUMO- or ubiquitin-signaling pathways. However, little is currently known regarding how these two post-translational modifications interact to control NR biology. We show that the SUMO and ubiquitin circuitry coordinately modifies pregnane x receptor (PXR, NR1I2) to play a key role in regulating PXR protein stability, transactivation capacity, and transcriptional repression. The SUMOylation and ubiquitylation of PXR is increased in a ligand- and tumor necrosis factor-alpha (TNFα)-dependent manner in hepatocytes. The SUMO-E3 ligase enzymes PIAS1 and PIASy drive high levels of PXR SUMOylation. Expression of PIAS1 selectively increases SUMO(3)ylation, as well as PXR-mediated induction of CYP3A and the xenobiotic response. The PIASy-mediated SUMO(1)ylation imparts a transcriptionally repressive function by ameliorating interaction of PXR with co-activator protein peroxisome proliferator-...

Research paper thumbnail of bi047367rsi20050124 043140

Research paper thumbnail of Venom variation and chemoreception of the viperid Agkistrodon contortrix: evidence for adaptation?

Journal of chemical ecology, 2003

Previous studies of chemoreceptive behavior in vipers suggest that snakes focus on the scent of e... more Previous studies of chemoreceptive behavior in vipers suggest that snakes focus on the scent of envenomated tissue to track their prey following envenomation. Other studies have indicated a correlation between qualitative differences in venom biochemistry and geographic variation in diet. The North American copperhead (Agkistrodon contortrix) varies geographically in diet and venom biochemistry; snakes were collected from three populations (Kansas, Texas, and Louisiana) that are known to have different prey preferences. Behavioral experiments were conducted to assess whether copperheads preferred envenomated prey more than nonenvenomated prey, as do other species of vipers studied thus far. Additional experiments tested the ability of copperheads to distinguish between envenomated prey from different geographic populations, and between geographic populations of copperheads and two other species of viper. Results indicated that copperheads prefer envenomated prey to nonenvenomated pr...

Research paper thumbnail of The host outer membrane proteins OmpA and OmpC are associated with the Shigella phage Sf6 virion

Virology, 2011

Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell c... more Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell components in phage assembly haven&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;t been well understood. It was previously reported that two unidentified proteins were present in bacteriophage Sf6 virion (Casjens et al, 2004, J.Mol.Biol. 339, 379-394, Fig. 2A). Using tandem mass spectrometry, we have identified the two proteins as outer membrane proteins (OMPs) OmpA and OmpC from its host Shigella flexneri. The transmission electron cryo-microscopy structure of Sf6 shows significant density at specific sites at the phage capsid inner surface. This density fit well with the characteristic beta-barrel domains of OMPs, thus may be due to the two host proteins. Locations of this density suggest a role in Sf6 morphogenesis reminiscent of phage-encoded cementing proteins. These data indicate a new, OMP-related phage:host linkage, adding to previous knowledge that some lambdoid bacteriophage genomes contain OmpC-like genes that express phage-encoded porins in the lysogenic state.

Research paper thumbnail of The standard molar enthalpy of formation of fluorofullereneC60F48

The Journal of Chemical Thermodynamics, 1999

The standard molar enthalpy of combustion of fluorinated fullereneC60F48 was determined by rotati... more The standard molar enthalpy of combustion of fluorinated fullereneC60F48 was determined by rotating-bomb calorimetry,ΔcHmo=−(24638 ± 163)kJ · mol−1. Using this result and the standard molar enthalpy of sublimation,ΔsubHmo= (109 ± 7)kJ · mol−1, the values of the standard molar enthalpies of formation of C60F48in the crystalline and gaseous states were found to be −(7563 ± 166)kJ · mol−1and −(7454 ±

Research paper thumbnail of Saturated vapor pressure and sublimation enthalpy of C60F 18

The Journal of Chemical Thermodynamics, 2002

... Cited By in Scopus (8). Permissions & Reprints. Regular Article. Saturated vapor pressure... more ... Cited By in Scopus (8). Permissions & Reprints. Regular Article. Saturated vapor pressure and sublimation enthalpy of C 60 F 18. Guido Gigli, a , 1 , Giovanni Balducci, a Vitaliy Yu. ... References. 1. AA Gakh, AA Tuinman, JL Adcock and RN Compton. Tetrahedron Lett. 34 (1993), pp ...

Research paper thumbnail of Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria

Research paper thumbnail of Direct fluorination of fullerenes

Russian Chemical Reviews, 2000

... According to mass spectroscopic data, the main products, viz., white powders, were mixtures o... more ... According to mass spectroscopic data, the main products, viz., white powders, were mixtures of fluorofuller-enes C60F38 ± 46 (Fig. 2); however, no perfluorinated fullerene C60F60 was detected, the maximum degree of fluorination being 48 (the C60F48 molecule). ...

Research paper thumbnail of Direct Infusion Mass Spectrometry of Oxylipin-Containing Arabidopsis Membrane Lipids Reveals Varied Patterns in Different Stress Responses

PLANT PHYSIOLOGY, 2012

Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized f... more Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized fatty acyl anions revealed 86 mass spectral peaks representing polar membrane lipids in extracts from Arabidopsis (Arabidopsis thaliana) infected with Pseudomonas syringae pv tomato DC3000 expressing AvrRpt2 (PstAvr). Quadrupole time-of-flight and Fourier transform ion cyclotron resonance mass spectrometry provided evidence for the presence of membrane lipids containing one or more oxidized acyl chains. The membrane lipids included molecular species of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyldiacylglycerol, monogalactosyldiacylglycerol, and acylated monogalactosyldiacylglycerol. The oxidized chains were identified at the level of chemical formula and included C 18 H 27 O 3 (abbreviated 18:4-O, to indicate four double bond equivalents and one oxygen beyond the carbonyl group), C 18 H 29 O 3 (18:3-O), C 18 H 31 O 3 (18:2-O), C 18 H 29 O 4 (18:3-2O), C 18 H 31 O 4 (18:2-2O), and C 16 H 23 O 3 (16:4-O)

Research paper thumbnail of Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis

Neurobiology of Aging, 2010

Brain aging is associated with a progressive decline in cognitive function though the molecular m... more Brain aging is associated with a progressive decline in cognitive function though the molecular mechanisms remain unknown. Functional changes in brain neurons could be due to age-related alterations in levels of specific proteins critical for information processing. Specialized membrane microdomains known as 'lipid rafts' contain protein complexes involved in many signal transduction processes. This study was undertaken to determine if two-dimensional fluorescence difference gel electrophoresis (2D DIGE) analysis of proteins in synaptic membrane lipid rafts revealed agedependent alterations in levels of raft proteins. Five pairs of young and aged rat synaptic membrane rafts were subjected to DIGE separation, followed by image analysis and identification of significantly altered proteins. Of 1046 matched spots on DIGE gels, 94 showed statistically significant differences in levels between old and young rafts, and 87 of these were decreased in aged rafts. The 41 most significantly altered (p < 0.03) proteins included several synaptic proteins involved in energy metabolism, redox homeostasis, and cytoskeletal structure. This may indicate a disruption in bioenergetic balance and redox homeostasis in synaptic rafts with brain aging. Differential levels of representative identified proteins were confirmed by immunoblot analysis. Our findings provide novel pathways in investigations of mechanisms that may contribute to altered neuronal function in aging brain.

Research paper thumbnail of A mass spectrometric study of C60F48

Mendeleev Communications, 1997

ABSTRACT