Naif Al-HArbi - Academia.edu (original) (raw)

Papers by Naif Al-HArbi

Background Liver diseases are major global health problems. Ginseng extract has antioxidant, immu... more Background Liver diseases are major global health problems. Ginseng extract has antioxidant, immune-modulatory and anti-inflammatory activities. This study investigated the effect of ginseng extract on carbon tetrachloride (CCl4)-induced liver fibrosis in rats. Methods Male Wistar rats were divided into four groups: control group, ginseng group, CCl 4 group and CCl 4 \u2009+\u2009ginseng group. Liver injury was induced by the intraperitoneal (I.P) injection of 3\ua0ml/kg CCl 4 (30% in olive oil) weekly for 8\ua0weeks. The control group was I.P injected with olive oil. The expression of genes encoding transforming growth factor beta ( TGF-\u3b2 ), type I TGF-\u3b2 receptor ( T\u3b2R-1 ), type II TGF-\u3b2 receptor ( T\u3b2R-II ), mothers against decapentaplegic homolog 2 ( Smad2 ), Smad3 , Smad4 , matrix metalloproteinase 2 ( MMP2 ), MMP9 , tissue inhibitor matrix metalloproteinase-1 ( TIMP-1 ), Collagen 1a2 ( Col1a2 ), Collagen 3a1 ( Col3a1 ), interleukin-8 ( IL-8 ) and interleukin ...

Journal of Medicinal Plants Research, Oct 23, 2011

Marjoram (Origanum majorana L., Family: Lamiaceae) is a common spicy medicinal herb, used as a ho... more Marjoram (Origanum majorana L., Family: Lamiaceae) is a common spicy medicinal herb, used as a home remedy for the treatment of different ailments. It is well recognized with its popular name "Marjoram". It is also used worldwide in food for better flavor, both in dry form and as fresh vegetable. Marjoram is a rich source of polyphenols which are known natural antioxidants. Marjoram ethyl alcohol extract treatment was evaluated for its effect on bone marrow, micronucleated polychromatic erythrocytes and selected biochemical parameters in mice. In addition, its protective role against the side effects of cyclophosphamide (CP, an established anticancer drug) was also studied. Marjoram treatment 125 mg/kg was found to be the minimum effective dose which could induce changes in some of the parameters investigated. However, CP treatment in mice brought highly significant changes in cytological as well as biological parameters included in the present study. Marjoram was added to CP treatment in order to evaluate the effects of CP and Marjoram combined treatment in mice. Marjoram was found to protect any changes occurring in RNA, DNA, and protein contents in the liver and testes of treated mice as compared with the control. It was interesting to notice that marjoram treatment did not interfere with the desired cytotoxic activity of CP.

BMC Biomedical Engineering, 2019

In the original publication of this article [1] the figures and captions were linked incorrectly.... more In the original publication of this article [1] the figures and captions were linked incorrectly. In this correction article the figures & captions are correctly published.

African Journal of Biotechnology, 2010

A study was undertaken to detect and monitor the degradation of hexadecane by three potential deg... more A study was undertaken to detect and monitor the degradation of hexadecane by three potential degrading bacteria (Pseudomonas putida, Rhodococcus erythroplolis and Bacillus thermoleovorans) isolated from contaminated soils in Riyadh, Saudi Arabia. The extraction of the bacterial populations from these polluted soils were 5.25 x 10 5 , 1.76 x 10 6 and 5.11 x 10 5 cells/ml, respectively with three different colony types of bacterial strains. Microbial population diversity studies were carried out by microbial enumeration identification and determination of growth responses of bacterial isolates in different concentrations of hexadecane. Phenotypic examination of the heterotrophic bacteria belonged mainly to the genus Pseudomonas, Rhodococcus and Bacillus. The mixed populations were capable of degrading hexadecane up to 120 ppm. The biodegradation of hexadecane by P. putida, R. erythroplolis and B. thermoleovorans together, was fast when compared to the biodegradation of hexadecane by each strain separately. This study reported on how to change contaminated soils to a non contaminated state. Healthy soils are essential not only to sustain production of food and fibber for citizens of the world, but also to provide a good quality life.

The Journal of Immunology, 2009

Topical application of imiquimod (IMQ), a TLR7/8 ligand and potent immune activator, can induce a... more Topical application of imiquimod (IMQ), a TLR7/8 ligand and potent immune activator, can induce and exacerbate psoriasis, a chronic inflammatory skin disorder. Recently, a crucial role was proposed for the IL-23/IL-17 axis in psoriasis. We hypothesized that IMQ-induced dermatitis in mice can serve as a model for the analysis of pathogenic mechanisms in psoriasis-like dermatitis and assessed its IL-23/IL-17 axis dependency. Daily application of IMQ on mouse back skin induced inflamed scaly skin lesions resembling plaque type psoriasis. These lesions showed increased epidermal proliferation, abnormal differentiation, epidermal accumulation of neutrophils in microabcesses, neoangiogenesis, and infiltrates consisting of CD4 ؉ T cells, CD11c ؉ dendritic cells, and plasmacytoid dendritic cells. IMQ induced epidermal expression of IL-23, IL-17A, and IL-17F, as well as an increase in splenic Th17 cells. IMQ-induced dermatitis was partially dependent on the presence of T cells, whereas disease development was almost completely blocked in mice deficient for IL-23 or the IL-17 receptor, demonstrating a pivotal role of the IL-23/IL-17 axis. In conclusion, the sole application of the innate TLR7/8 ligand IMQ rapidly induces a dermatitis closely resembling human psoriasis, critically dependent on the IL-23/IL-17 axis. This rapid and convenient model allows further elucidation of pathogenic mechanisms and evaluation of new therapies in psoriasis.

Background Liver diseases are major global health problems. Ginseng extract has antioxidant, immu... more Background Liver diseases are major global health problems. Ginseng extract has antioxidant, immune-modulatory and anti-inflammatory activities. This study investigated the effect of ginseng extract on carbon tetrachloride (CCl4)-induced liver fibrosis in rats. Methods Male Wistar rats were divided into four groups: control group, ginseng group, CCl 4 group and CCl 4 \u2009+\u2009ginseng group. Liver injury was induced by the intraperitoneal (I.P) injection of 3\ua0ml/kg CCl 4 (30% in olive oil) weekly for 8\ua0weeks. The control group was I.P injected with olive oil. The expression of genes encoding transforming growth factor beta ( TGF-\u3b2 ), type I TGF-\u3b2 receptor ( T\u3b2R-1 ), type II TGF-\u3b2 receptor ( T\u3b2R-II ), mothers against decapentaplegic homolog 2 ( Smad2 ), Smad3 , Smad4 , matrix metalloproteinase 2 ( MMP2 ), MMP9 , tissue inhibitor matrix metalloproteinase-1 ( TIMP-1 ), Collagen 1a2 ( Col1a2 ), Collagen 3a1 ( Col3a1 ), interleukin-8 ( IL-8 ) and interleukin ...

Journal of Medicinal Plants Research, Oct 23, 2011

Marjoram (Origanum majorana L., Family: Lamiaceae) is a common spicy medicinal herb, used as a ho... more Marjoram (Origanum majorana L., Family: Lamiaceae) is a common spicy medicinal herb, used as a home remedy for the treatment of different ailments. It is well recognized with its popular name "Marjoram". It is also used worldwide in food for better flavor, both in dry form and as fresh vegetable. Marjoram is a rich source of polyphenols which are known natural antioxidants. Marjoram ethyl alcohol extract treatment was evaluated for its effect on bone marrow, micronucleated polychromatic erythrocytes and selected biochemical parameters in mice. In addition, its protective role against the side effects of cyclophosphamide (CP, an established anticancer drug) was also studied. Marjoram treatment 125 mg/kg was found to be the minimum effective dose which could induce changes in some of the parameters investigated. However, CP treatment in mice brought highly significant changes in cytological as well as biological parameters included in the present study. Marjoram was added to CP treatment in order to evaluate the effects of CP and Marjoram combined treatment in mice. Marjoram was found to protect any changes occurring in RNA, DNA, and protein contents in the liver and testes of treated mice as compared with the control. It was interesting to notice that marjoram treatment did not interfere with the desired cytotoxic activity of CP.

BMC Biomedical Engineering, 2019

In the original publication of this article [1] the figures and captions were linked incorrectly.... more In the original publication of this article [1] the figures and captions were linked incorrectly. In this correction article the figures & captions are correctly published.

African Journal of Biotechnology, 2010

A study was undertaken to detect and monitor the degradation of hexadecane by three potential deg... more A study was undertaken to detect and monitor the degradation of hexadecane by three potential degrading bacteria (Pseudomonas putida, Rhodococcus erythroplolis and Bacillus thermoleovorans) isolated from contaminated soils in Riyadh, Saudi Arabia. The extraction of the bacterial populations from these polluted soils were 5.25 x 10 5 , 1.76 x 10 6 and 5.11 x 10 5 cells/ml, respectively with three different colony types of bacterial strains. Microbial population diversity studies were carried out by microbial enumeration identification and determination of growth responses of bacterial isolates in different concentrations of hexadecane. Phenotypic examination of the heterotrophic bacteria belonged mainly to the genus Pseudomonas, Rhodococcus and Bacillus. The mixed populations were capable of degrading hexadecane up to 120 ppm. The biodegradation of hexadecane by P. putida, R. erythroplolis and B. thermoleovorans together, was fast when compared to the biodegradation of hexadecane by each strain separately. This study reported on how to change contaminated soils to a non contaminated state. Healthy soils are essential not only to sustain production of food and fibber for citizens of the world, but also to provide a good quality life.

The Journal of Immunology, 2009

Topical application of imiquimod (IMQ), a TLR7/8 ligand and potent immune activator, can induce a... more Topical application of imiquimod (IMQ), a TLR7/8 ligand and potent immune activator, can induce and exacerbate psoriasis, a chronic inflammatory skin disorder. Recently, a crucial role was proposed for the IL-23/IL-17 axis in psoriasis. We hypothesized that IMQ-induced dermatitis in mice can serve as a model for the analysis of pathogenic mechanisms in psoriasis-like dermatitis and assessed its IL-23/IL-17 axis dependency. Daily application of IMQ on mouse back skin induced inflamed scaly skin lesions resembling plaque type psoriasis. These lesions showed increased epidermal proliferation, abnormal differentiation, epidermal accumulation of neutrophils in microabcesses, neoangiogenesis, and infiltrates consisting of CD4 ؉ T cells, CD11c ؉ dendritic cells, and plasmacytoid dendritic cells. IMQ induced epidermal expression of IL-23, IL-17A, and IL-17F, as well as an increase in splenic Th17 cells. IMQ-induced dermatitis was partially dependent on the presence of T cells, whereas disease development was almost completely blocked in mice deficient for IL-23 or the IL-17 receptor, demonstrating a pivotal role of the IL-23/IL-17 axis. In conclusion, the sole application of the innate TLR7/8 ligand IMQ rapidly induces a dermatitis closely resembling human psoriasis, critically dependent on the IL-23/IL-17 axis. This rapid and convenient model allows further elucidation of pathogenic mechanisms and evaluation of new therapies in psoriasis.