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Papers by Nasrollah Sohrabi

Comparative Immunology, Microbiology and Infectious Diseases

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Asian Journal of Pharmaceutics, Sep 14, 2018

Background and Objective: As Cytomegalovirus (CMV) has been distributed widely and great number o... more Background and Objective: As Cytomegalovirus (CMV) has been distributed widely and great number of people are affected by it, such as hemophilic patients, and given the prevalence and complications of CMV, hepatitis B, hepatitis C, and HIV and lack of study in Kurdistan province in this regard, this study was conducted to evaluate the prevalence of CMV infection and other viruses among the hemophilic patients. This study can be used to reduce and solve the problem of hemophilic patients and health system plans. The objective of this study was to evaluate the prevalence of mentioned infections among hemophilic patients in Besat Hospital in Sanandaj in 2017. Materials and Methods: This is a descriptive study in which its population included all 121 hemophilic patients, diagnosed in Besat Hospital of Kurdistan province. They were examined in terms of infection of four viruses, including hepatitis C, hepatitis B, CMV, and HIV. It should be noted that the definition of infection was based on global criteria. Results: Of 121 patients, 95 cases were tested for hepatitis C, which 22 of them (23.1%) were positive in terms of HCV-Ab, and 13 people (13.6%) were suspected. In addition, 88 of them were tested for CMV, which 70 of them (79.5%) were positive in terms of CMV-Ab. In terms of HIV and HBV, all tested patients were seronegative. Among these patients, 48 patients had severe hemophilia, who recurrently received coagulation factor. Among them, 14 people (29. 1%) were seronegative in terms of HCV-Ab, and 43 people (89.5%) were seronegative in terms of CMV-Ab. In addition, nine of them (18.7%) were suspected in terms of HCV-Ab.Conclusion: The recent st u dy showed that the prevalence of hepatitis C among hemophilic patients is higher than that in other people of the community. In addition, among hemophilic patients, the prevalence of hepatitis C is higher in patients who received coagulation factor recurrently than that in other patients. The prevalence of CMV is also more in patients receiving factor recurrently than that in rest of the patients. Therefore, patients receiving blood and blood products should be periodically evaluated for virology.

Journal of Research in Medical and Dental Science, 2018

Microbial resistance is considered as one of the challenges in coping with infectious diseases. T... more Microbial resistance is considered as one of the challenges in coping with infectious diseases. Thus, to find or synthesize new antimicrobial substances is very important. Copper oxide (CuO) has drawn the attention of the researchers due to its antibacterial effect in coping with microbial resistance. In this research, the antibacterial effects of copper oxide nanoparticles on the standard and clinical strains of Pseudomonas aeruginosa, which its new strains have been associated with microbial resistance, were examined and compared. In this experimental-interventional research, a copper oxide nanoparticle was synthesized at a size of 33 nm using copper sulfate chemical reduction method. Then, the antibacterial effects of copper oxide nanoparticle on standard strain (ATCC 27853) and clinical strain of Pseudomonas aeruginosa were examined using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bacteria death kinetics. Data derived from investigating...

Metallo-b-lactamase (MBL)-producing Acinetobacter baumannii has become a growing therapeutic conc... more Metallo-b-lactamase (MBL)-producing Acinetobacter baumannii has become a growing therapeutic concern worldwide. The aims of this study were to evaluate the antimicrobial susceptibility of A. baumannii isolates and to determine the prevalence of MBL genes among carbapenem nonsusceptible isolates. During a period of 16 months (March 2008-June 2009), 100 isolates of A. baumannii were collected from different clinical specimens of inpatients admitted to the largest teaching hospital in the northwest of Iran. All isolates were tested for antimicrobial susceptibility by Kirby-Bauer disk diffusion method. Carbapenem non-susceptible isolates were further screened for production of MBL by Etest and were then subjected to PCR for detection of MBL genes of types bla IMP and bla VIM. Among 63 carbapenem (imipenem and meropenem) non-susceptible isolates of A. baumannii, 31 (49z) were found to be MBL producers. Of 31 MBL-producing isolates, 19 (61z) carried the bla IMP gene and 9 (29z) carried the bla VIM gene. All MBL-producing isolates were multidrug resistant. This is the first report of IMP and VIM types among MBL-producing A. baumannii in Iran.

Asian Journal of Pharmaceutics, 2018

Background: One of the main causes of gastric ulcer and inflammation is Helicobacter pylori (H. p... more Background: One of the main causes of gastric ulcer and inflammation is Helicobacter pylori (H. pylori). This bacterium has a global spread. The objective of the present research is to evaluate the genotype relationship between manganese superoxide dismutase (MnSOD) Ala16Val and the risk of the gastric ulcer. Materials and Methods: This case–control research was conducted on 75 patients with gastric ulcer and 60 healthy individuals as a control. By using the kit, DNA was extracted from gastric paraffin blocks and control group blood samples. Polymerase chain reaction was implemented to detect glmM and MnSOD genes, and restriction fragment length polymorphism was used to examine MnSOD Ala16Val polymorphism. Results: The frequency of glmM gene in patients with gastric ulcer was positive and was 74.66%. Analyzing MnSOD Ala16Val polymorphism indicated that the A/V frequency was higher in both patient and control groups. In addition, the allelic analysis indicated that the allele A was s...

Iranian Journal of Public Health, 2014

Pharmacology & Therapeutics, 2020

HMG-CoA reductase inhibitors, or statins, are potent plasma LDL-cholesterol (LDL-c) lowering agen... more HMG-CoA reductase inhibitors, or statins, are potent plasma LDL-cholesterol (LDL-c) lowering agents. Since the introduction of the first statin, lovastatin, in 1987, accumulating evidence showed that non-cholesterol lowering effects play an important role in their efficacy to reduce atherosclerotic cardiovascular disease (ASCVD). Thus, these non-LDL-c lowering properties could benefit patients with immune-mediated diseases. Statins and their associated immune-modulating roles have recently received much attention. Different statins have been administered in various experimental and clinical studies focused on autoimmunity. The results indicate that statins can modulate immune responses through mevalonate pathway-dependent and-independent mechanisms. The antiinflammatory and immune-modulating effects include cell adhesion, migration of antigen presenting cells, and differentiation, as well as activation, of T-cells. In various autoimmune diseases (e.g. rheumatoid arthritis, lupus, and multiple sclerosis), promising results have been obtained to date.

Jundishapur Journal of Microbiology, 2020

Background: Methicillin-resistant Staphylococcus aureus (MRSA) isolates are important agents of h... more Background: Methicillin-resistant Staphylococcus aureus (MRSA) isolates are important agents of human bacterial infections. The mecA gene as the main cause of resistance against beta-lactams is located in genetic elements which are known as staphylococcal cassette chromosome mec (SCCmec). Objectives: The research aimed to evaluate the antibiotic susceptibility pattern and SCCmec typing of MRSA isolates in Kermanshah Province, West of Iran. Methods: Identification of MRSA isolates were done using phenotypic and genotypic methods. Antimicrobial susceptibility patterns were determined using the disk diffusion method and minimum inhibitory concentration (MIC) testing by agar dilution method. The SCCmec types of isolates were identified using PCR method. The results of the research were analyzed using SPSS.V16 software. Results: In this research, of 146 isolates, 126 isolates were confirmed as S. aureus using phenotypic methods and PCR analysis of femB gene. All isolates were sensitive to vancomycin by both methods: disk agar diffusion and MIC testing by agar dilution method. The highest resistance rate was related to erythromycin (75.4%) and ciprofloxacin (73%). Of 126 S. aureus isolates, 83 cases (65.9%) and 81 cases (64.3%) were MRSA based on the existence of mecA gene and cefoxitin diffusion disk test, respectively. There was a statistically significant difference in antibiotic susceptibility pattern of MRSA and methicillin susceptible Staphylococcus aureus (MSSA) isolates for some antibiotics such as gentamicin, amikacin, erythromycin, ciprofloxacin, and linezolid (P < 0.05). SCCmec types were detected as 20 cases (24.1%) type I, 5 cases (6%) type II, 37 cases (44.6%) were type III (the most prevalent type), 6 cases (7.2%) type IVa, and 3 cases (3.6%) type IV. The prevalence of HA-MRSA (types I, II, and III) and CA-MRSA (types IV and V) in this study were 74.7% and 10.8%, respectively. Conclusions: The prevalence of MRSA isolates is high in Kermanshah Province, West of Iran. The cefoxitin diffusion disk testing could be considered a simple, cheap and reliable test for identification of MRSA isolates in all laboratories. The most frequent type of SCCmec is type III. These findings could be due to an increase in antibiotic consumption and insufficient infection control systems.

Microbial Pathogenesis, 2017

Pseudomonas aeruginosa is an opportunistic human pathogen especially in patients with underlying ... more Pseudomonas aeruginosa is an opportunistic human pathogen especially in patients with underlying diseases such as cyctic fibrosis and has been established as a model organism to study bacterial biofilm formation. The aim of this study was to compare the biofilm formation and antibiotic resistance in human and environmental P. aeruginosa isolates. Numbers of positive samples for algD and algU genes in human samples were 98% and the positive samples for algD and algU genes in the environmental samples were 80% and 70%, respectively. Ability to create biofilms by the human and environmental samples were 70% and 28%, respectively. The incidences of various antibiotic resistance genes in human samples including bla TEM and bla SHV were 92% and 16%, respectively but antibiotic resistance genes in environmental samples including bla TEM and bla SHV were 20% and 6%, respectively. High resistance to gentamicin (74%) and meropenem (70%), were found in the human samples, were as in the environmental samples high level of resistance were observed to ceftazidime (30%), gentamicin and meropenem (28%). According to findings of this study, differences in genes involve in biofilm synthesis between human and environmental isolates are highly significant and the environmental isolates of P. aeruginosa stile are sensitive to most antibiotics because they lacks the antibiotic resistance genes. But after transfer to human and isolation from diseased people have been taken the antibiotic resistance genes that would be resistant to many antibiotics.

The First International Congress of Medical Bacteriology, Sep 6, 2011

Background and Objectives: In recent years, multidrug-resistant (MDR) A. baumannii has become a m... more Background and Objectives: In recent years, multidrug-resistant (MDR) A. baumannii has become a major clinical concern because of its different resistance patterns and clonal spread of resistant isolates. The aim of this study was to evaluate possible clonal relationship of MDR A. baumannii isolates by repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) and Sequence-type multiplex-PCR in Imam Reza hospital, Tabriz. Materials & Methods: A total of 80 MDR A. baumannii isolates were collected from different clinical specimens during a 1-year period. Species identification was confirmed by detection of blaOXA-51-like gene. Three sets sequence-type multiplex-PCR assays were performed to assign multidrug-resistant A. baumannii belonging to particular genotypes of sequence groups (European clone I, II and III).Genetic relationship of MDR strains was also evaluated by REP-PCR and then using cluster analysis method. Results: Sequence-type multiplex-PCR showed that 63 (79%) of multidrug resistant isolates belonged to group 1(European clone II), 14 isolates (17.5%) belonged to group 2 (European clone I) and 3 isolates (4%) were belonged to non European clones I, II and III. None were belonged to group3 (European clone 3). REP-PCR revealed that multidrug-resistant isolates belonged to three distinct main genotypes which confirmed this clustering. Conclusion: We found clonal spread and circulation of MDR A. baumannii isolates (more importantly corresponding to EU clone II) in different wards of our hospital. European clone II seems to be particularly successful in its spread in non-European countries. Early recognition of strains belonging to clones with high transmissibility potential would be beneficial by leading to the implementation of infection control measures in order to prevent further spread in hospital.

Journal of Research in Medical and Dental Science, 2018

With ongoing use of preservative chemical compounds in the food industry, in addition to the chal... more With ongoing use of preservative chemical compounds in the food industry, in addition to the challenge of microbial resistance, also there is growing concerns over side effects of these compounds. So, the use of herbal essential oils in food preservatives with much less side effects and sometimes even positive effects, has been considered. Therefore, in the present study, the antibacterial effects of Nisin and Satureja montana essential oil have been studied separately and in combination on standard strains of Escherichia coli and Staphylococcus aureus, that are important bacteria in food microbial contamination. Constituents analysis of Satureja essential oil was performed by GC/MS. Antimicrobial susceptibility testing as Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was performed by Broth micro dilution method and evaluation of interactions between the compounds, was performed by calculating FIC index (Fractional Inhibitory Concentration) meth...

Pak J Med Sci October …, 2010

Pak J Med Sci 2010 Vol. 26 No. 4 www.pjms.com.pk 769 ... Nasrollah Sohrabi1, Mohammad Taghi Akhi2... more Pak J Med Sci 2010 Vol. 26 No. 4 www.pjms.com.pk 769 ... Nasrollah Sohrabi1, Mohammad Taghi Akhi2, Safar Farajnia3, Mohammad Reza Nahaei4, Mohammad Ahangarzadeh Rezaei5 ... ABSTRACT Objective: To determine the prevalence of PER-1 gene type ESBLs ...

Microbial Drug Resistance, 2012

Journal Ni, Feb 24, 2015

Background and Objective: Production of extended-spectrum ßlactamases (ESBLs) is the major cause ... more Background and Objective: Production of extended-spectrum ßlactamases (ESBLs) is the major cause of resistance to β-lactam antibiotics in Escherichia coli. In this study, the antibiotic susceptibility pattern and the prevalence of ESBL-producing E. coli isolates were studied in a hospital in Kermanshah, western Iran. Methods: E. coli isolates were identified using microbiological and biochemical standard tests. Antibiotic susceptibility testing and the detection of ESBL-producing E. coli isolates were done according to Clinical and Laboratory Standards Institute (CLSI) criteria. Results: The most frequent isolation source of E. coli clinical isolates was urine (94.1%). Most of the isolates were resistant against ampicillin (88.2%). Imipenem and amikacin were the most active antibiotics having 81.2% and 78.8% activity, respectively. Results of antimicrobial susceptibility showed that 57.6% of isolates were multidrug resistant (MDR). Results of Combined Disk Test (CDT) showed that 56.5% of isolates were ESBL positive, and among MDR isolates, 79.6% were ESBL producers. There was a significant difference in ESBL MDR patterns between ESBL producers and non-producer ESBL E.coli isolates (p<0.05).

International Journal of Hematology Oncology and Stem Cell Research, Feb 12, 2013

Introduction: ITP is an autoimmune blood disorder in which platelet destruction is mediated by an... more Introduction: ITP is an autoimmune blood disorder in which platelet destruction is mediated by antiplatelet antibodies. The mechanisms of anti-platelet antibodies development are still a little known. The rate of some bacterial or viral agents in cause of ITP is well known. Recently, some study proposed that H pylori infection may be associated with ITP and H pylori eradication can improves platelet counts in infected ITP patients. Material and method: A baseline platelet count <50×103 µL for 4 weeks prior to study entry were required. These patients were tested for H. pylori infection by Urea Breath. All positive H pylori patients received triple therapy for 7 or 14 days to eradicate H pylori infection. These patients followed for six months. Results: Of 92 patients with ITP, H pylori infection was found in 59.7% (55/92). After excluding patients with confounding factors, 41 patients were remained. After H pylori eradication, CR wasn't obtained in any patients. Partial response were obtained only in 3 (7.3%) of the 41 patients and no response in 38 (92.6%) patients. There is a significant difference between the platelet counts of PR and NR groups (P<0.001). Conclusion: the results of this study and our previously study showed H pylori eradication therapy has beneficial effect for patients with mild thrombocytopenia but the chance of obtaining a response by H pylori treatment is lower in patients with severe thrombocytopenia.

Journal Ni, Apr 23, 2014

In hospital facilities the quality of indoor air is a critical factor in the prevention of infect... more In hospital facilities the quality of indoor air is a critical factor in the prevention of infections. Poor quality air inside hospitals may lead to hospital-acquired infections, sick building syndrome, and various occupational risks. The aim of this study was to determine the level of airborne fungal contamination in a referral burn center in Western Iran, affiliated to Kermanshah University in Medical Sciences. Methods: This study was performed in the burn ward of Imam Khomeini Hospital of Kermanshah University of Medical Sciences, in July 2013. Samples were collected from four different parts of the ward, including; public rooms (31 samples), corridor (10 samples), toilet (9 samples), and dressing room (8 samples). Open lid petri dishes, containing Sabouraud dextrose agar medium, were held approximately 1.5 meters from the floor for 15 minutes. The airborne fungi were identified using mycological methods. Descriptive analysis of data was performed using SPSS software (version 16). Results: Out of a total of 58 culture samples, 38 were positive for fungi. There were 54 colonies from six fungal genera. The most prevalent fungal species were Penicillium spp. with 18 colonies (33.3%), followed by Aspergillus spp. with 13 (24%) colonies. Public rooms were the most contaminated areas (93.5%), while the dressing room (25%) was the cleanest area. The rate of contamination in the dressing room and other areas of the burn ward were statistically significant (P<0.01).

Japanese Journal of Infectious Diseases, 2011

Metallo-beta-lactamase (MBL)-producing Acinetobacter baumannii has become a growing therapeutic c... more Metallo-beta-lactamase (MBL)-producing Acinetobacter baumannii has become a growing therapeutic concern worldwide. The aims of this study were to evaluate the antimicrobial susceptibility of A. baumannii isolates and to determine the prevalence of MBL genes among carbapenem non-susceptible isolates. During a period of 16 months (March 2008-June 2009), 100 isolates of A. baumannii were collected from different clinical specimens of inpatients admitted to the largest teaching hospital in the northwest of Iran. All isolates were tested for antimicrobial susceptibility by Kirby-Bauer disk diffusion method. Carbapenem non-susceptible isolates were further screened for production of MBL by Etest and were then subjected to PCR for detection of MBL genes of types bla(IMP) and bla(VIM). Among 63 carbapenem (imipenem and meropenem) non-susceptible isolates of A. baumannii, 31 (49%) were found to be MBL producers. Of 31 MBL-producing isolates, 19 (61%) carried the bla(IMP) gene and 9 (29%) carried the bla(VIM) gene. All MBL-producing isolates were multidrug resistant. This is the first report of IMP and VIM types among MBL-producing A. baumannii in Iran.

Polish Journal of Microbiology Polskie Towarzystwo Mikrobiologow the Polish Society of Microbiologists, 2012

Integrons are associated with a variety of gene cassettes, which confer resistance to multiple cl... more Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibacterial drugs. In this study we tested the frequency of class 1 and 2 integrons among multidrug-resistant Acinetobacter baumannii (MDRAB) clinical isolates. One hundred clinical isolates of A. baumannii were screened for carriage of class 1 and 2 integrons by PCR method. Results showed that seventy four (92.5%) of 80 MDRAB carried class 1 integron. Integron-positive isolates were statistically more resistant to aminoglycoside, quinolone and beta-lactam compounds except for cefepime. This is the first report of class 1 integrons in MDRAB isolates in northwest Iran.

Japanese journal of infectious diseases, 2011

Comparative Immunology, Microbiology and Infectious Diseases

Asian Journal of Pharmaceutics, Sep 14, 2018

Journal of Research in Medical and Dental Science, 2018

Asian Journal of Pharmaceutics, 2018

Iranian Journal of Public Health, 2014

Pharmacology & Therapeutics, 2020

Jundishapur Journal of Microbiology, 2020

Microbial Pathogenesis, 2017

The First International Congress of Medical Bacteriology, Sep 6, 2011

Journal of Research in Medical and Dental Science, 2018

Pak J Med Sci October …, 2010

Microbial Drug Resistance, 2012

Journal Ni, Feb 24, 2015

International Journal of Hematology Oncology and Stem Cell Research, Feb 12, 2013

Journal Ni, Apr 23, 2014

Japanese Journal of Infectious Diseases, 2011

Polish Journal of Microbiology Polskie Towarzystwo Mikrobiologow the Polish Society of Microbiologists, 2012

Japanese journal of infectious diseases, 2011