Nasserdine Sabaou - Academia.edu (original) (raw)
Papers by Nasserdine Sabaou
Chemistry & biodiversity, 2017
In the present work, the Brassica rapa var. rapifera parts essential oils and their antioxidant a... more In the present work, the Brassica rapa var. rapifera parts essential oils and their antioxidant and antimicrobial activities were investigated for the first time depending on geographic origin and extraction technique. Gas-chromatography (GC) and GC/mass spectrometry (MS) analyses showed several constituents, including alcohols, aldehydes, esters, ketones, norisoprenoids, terpenic, nitrogen and sulphur compounds, totalizing 38 and 41 compounds in leaves and root essential oils, respectively. Nitrogen compounds were the main volatiles in leaves essential oils and sulphur compounds were the main volatiles in root essential oils. Qualitative and quantitative differences were found among B. rapa var. rapifera parts essential oils collected from different locations and extracted by hydrodistillation and microwave-assisted hydrodistillation techniques. Furthermore, our findings showed a high variability for both antioxidant and antimicrobial activities. The highlighted variability reflect...
African Journal of Microbiology Research, 2013
A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution... more A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution agar plating method using a chitin-vitamins B medium supplemented with different antibiotics as selective agents. This actinomycete produced antimicrobial substances and exhibited strong antifungal activity against mycotoxigenic fungi including Aspergillus carbonarius (M333) and Penicillium expansum, and also against phytopathogenic fungi such as Fusarium oxysporum f. sp. lini (Foln 3-5) and F. culmorum. In addition, the strain showed strong activity against the yeast Saccharomyces cerevisiae (ATCC 4226). On the other hand, the strain exhibited an interesting antibacterial activity against bacteria including multi-resistant Klebsiella pneumoniae (E40). The highest antimicrobial activities were obtained on ISP2 medium. The n-butanol extract contained three bioactive spots detected on thin layer chromatography (TLC) plates. Strain PP14 was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Streptomyces. The 16S rRNA gene sequence similarities showed that strain PP14 is closely associated with members of the Streptomyces violaceoruber species group (S. violaceoruber, Streptomyces violaceolatus, Streptomyces tricolor, Streptomyces humiferus, Streptomyces coelescens and Streptomyces anthocyanicus). Furthermore, the comparison of physiological characteristics of strain PP14 with the S. violaceoruber species group showed significant differences. Our results showed that strain PP14 represents a distinct phyletic line suggesting a new genomic species.
Chemistry & Biodiversity, 2016
The impact of phenological stages (vegetative, flowering and fruiting stages) on chemical composi... more The impact of phenological stages (vegetative, flowering and fruiting stages) on chemical composition, antioxidant, and antimicrobial activities of Tetraclinis articulata (Vahl) Mast. parts essential oils were investigated for the first time. GC and GC/MS analyses pointed to a quantitative variability of components; terpene hydrocarbons derivatives, represented by α-pinene (16.38 ± 0.19 - 31.78 ± 0.35%) and limonene (3.41 ± 0.07 - 9.49 ± 0.14%) as major components, predominate at the vegetative stage, whereas oxygenated derivatives, represented by camphor (16.11 ± 0.23 - 26.17 ± 0.29%) and bornyl acetate (15.21 ± 0.21 - 27.33 ± 0.33%) as major components, predominate at the fruiting stage. Furthermore, our findings showed that the plant parts collected at the fruiting stage possess the highest antioxidant activity and the best antimicrobial activity against the tested microorganisms, than plant parts collected at the vegetative and flowering stages. This highlighted variability reflects the high impact of phenological cycle on chemical composition and biological activities, which led to conclude that we should select essential oils to be investigated carefully depending on phenological stage, in order to have the highest effectiveness of essential oil in terms of biological activities for human health purposes.
Antonie van Leeuwenhoek, 2016
A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample ... more A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3(T). The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C17:1ω9c (38.6%) and C17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3(T) shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632(T) (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177(T) (99.2%) and P. parontospora subsp. antibiotica DSM 43869(T) (99.0%). DNA-DNA hybridization values between strain PM3(T) and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3(T) represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3(T) (=DSM 46752(T) = CECT 9047(T)).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterial strain, designated MB27T, was isolated from a Saharan soil sample collect... more A novel actinobacterial strain, designated MB27T, was isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria). Strain MB27T was characterized following a polyphasic taxonomic approach. This strain produced a branched and fragmented substrate mycelium, which was found to have a yellowish orange colour. A white scanty aerial mycelium was produced on most media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB27T belongs to the family Pseudonocardiaceae and is closely related to the genus Saccharothrix. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose, ribose and small amounts of mannose and rhamnose. The detected phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Mycolic acids were not detected while the predominant fatty acid was iso-branched hexadecanoate (iso-C16 : 0). The major menaquinone was MK-9(H4). Results of 16S rRNA gene sequence comparisons revealed that strain MB27T shairs the highest degree of similarity with Saccharothrix ecbatanensis DSM 45486T (99.8%), Saccharothrix hoggarensis DSM 45457T (99.3 %), Saccharothrix longispora DSM 43749T (98.6 %) and Saccharothrix yanglingensis DSM 45665T (98.6 %). However, it exhibited only 11-42 % DNA-DNA relatedness to the neighbouring Saccharothrixspecies. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB27T is shown to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix isguenensis sp. nov. (type strain MB27T=DSM 46885T=CECT 9045T) is proposed.
Biocontrol Science and Technology, 2016
Canadian Journal of Microbiology, 1987
Abstract.-This study aims to demonstrate the therapeutic advantage of traditional date vinegar. W... more Abstract.-This study aims to demonstrate the therapeutic advantage of traditional date vinegar. We carried out an ethno-pharmacological survey on the therapeutic use of date vinegar by the population of Ghardaïa. After we verified the quality of two vinegars made from Deglet-Nour and Temjouhart date varieties, we tested their antimicrobial activity on Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. These microorganisms are involved in skin infections treated with date vinegar. The ethno-pharmacological survey showed that the traditional date vinegar is used in the treatment of 30 afflictions. The treatment seems to be effective (75% of only positive effects compared to 14% of positive and also adverse effects). The antimicrobial activity was present, in spite of the weak concentration of acetic acid in the tested vinegars. The highest antimicrobial effect was observed on S. aureus. The inhibition diameter of this microorganism was 49 mm in Deglet-Nour vinegar and 33 mm in Temjouhart vinegar. In conclusion, date vinegar is used by the population of Ghardaïa to treat various illnesses and it was proven that it is effective in the treatment of skin infections caused by microorganisms mentioned previously. Résumé.-Le but de cette étude est de démontrer l'intérêt thérapeutique du vinaigre traditionnel de dattes. Nous avons mené une étude ethno-pharmacologique sur le traitement par le vinaigre de deux variétés de dattes auprès de la population de Ghardaïa. En suite, nous avons vérifié la qualité de deux vinaigres fabriqués à partir des dattes de Deglet-Nour et de Temjouhart, puis testé leur activité antimicrobienne contre Staphylococcus aureus, Pseudomonas aeruginosa et Candida albicans. Ces germes sont impliqués dans les infections de la peau traitées par le vinaigre de dattes. Les résultats ont montré que le vinaigre traditionnel de dattes est utilisé dans le traitement de 30 affections. Le traitement semble être efficace (75% de résultats positif et sans aucun effet indésirable par rapport à 14% de résultats positifs mais avec des effets indésirables). L'activité antimicrobienne était présente, malgré la concentration faible en acide acétique dans les deux vinaigres. L'effet antimicrobien le plus fort a été observé contre S. aureus avec un diamètre d'inhibition de 49 mm chez le vinaigre de Deglet-Nour et de 33 mm chez le vinaigre de Temjouhart. On a conclu que le vinaigre de dattes est utilisé par la population de Ghardaïa pour traiter diverses maladies et il s'est avéré efficace dans le traitement des infections de la peau causées par les germes précités. Mots clés: vinaigre de dattes, ethno-pharmacologie, activité antimicrobienne, infections de la peau, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans. Ethno-pharmocological use and antimicrobial activity of traditional date vinegar of Ghardaïa
Journal of Plant Diseases and Protection, 2015
International Journal of Systematic and Evolutionary Microbiology, 2016
Throughout our investigations for new actinobacterial taxa, a new actinobacterial strain, designa... more Throughout our investigations for new actinobacterial taxa, a new actinobacterial strain, designated MB20T, was isolated from a Saharan soil sample, collected in Mzab region (Ghardaïa province, South Algeria). In order to reveal its taxonomic position, the novel strain was characterized following a polyphasic taxonomic approach. It was noticed that strain MB20T produce white branched and fragmented substrate mycelium with no aerial mycelium on the most tested media. Chemotaxonomic and phylogenetic studies clearly demonstrated that the strain MB20T belonged to the family Pseudonocardiaceae and was closely related to the genus Actinophytocola. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose and ribose. The diagnostic phospholipid was phosphatidylethanolamine. Mycolic acids were not detected while the predominant fatty acid was found to be iso-branched hexadecanoate (iso-C16 : 0). The major menaquinone was MK-9(H4). Results of the 16S rRNA gene sequence comparison revealed that strain MB20T shared the highest degree of similarity with Actinophytocola gilvus DSM 45828T (98.5 %), Actinophytocola corallina DSM 45659T (98.0 %) and Actinophytocola timorensis DSM 45660T (97.5 %). However, DNA-DNA hybridization studies showed only 32.9 % similarity with A. timorensis, 23.7 % similarity with A. gilvus and 17.9 % similarity with A. corallina. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB20T revealed to be a novel species of the genus Actinophytocola, for which the name Actinophytocola algeriensis sp. nov. (type strain MB20T =DSM 46746T = CECT 8960T) is proposed.
International Journal of Systematic and Evolutionary Microbiology, 2016
The taxonomic position of a novel actinobacterium, strain SG1T, isolated from a Saharan soil samp... more The taxonomic position of a novel actinobacterium, strain SG1T, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria) was established by using a polyphasic approach. The microorganism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones was MK-9(H2) and MK-9(H4). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C17 : 1 ω8c, iso-C16 : 0, 10-methyl C17 : 0, C18 : 1 ω9c and C17 : 0. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was most closely related to 'Streptosporangium subfuscum' DSM 46724T (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181T (98.7 %), Streptosporangium fragile DSM 43847T (98.6 %) and Streptosporangium sandarakinum DSM 45763T (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1T formed a cluster with its closest relative 'S. subfuscum' DSM 46724T. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1T could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1T should be classified as the type strain of a novel species in the genus Streptosporangium, for which the name Streptosporangium becharense sp. nov., is proposed. The type strain is SG1T (=DSM 46887T =CECT 8961T).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collec... more A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collected from Adrar province, South of Algeria. A polyphasic study was carried out to establish the taxonomic position of this strain. Strain ACD12T was observed to form extensively branched substrate mycelia. Aerial mycelium was absent or weakly produced on all tested media, while spore chains are short with hooked and irregular spirals forms (2-3 turns). The dominant diaminopimelic acid isomer in the cell wall was meso-diaminopimelic acid. Glucose, ribose, galactose, mannose and madurose occur in whole-cell hydrolysates. The major phospholipid was diphosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H6). The fatty acid profile was characterized by the presence of C16:0, C17:0, C15:0, C18:0, C18:1 cis9 and iso-C16:0. Results of 16S rRNA gene sequence comparison revealed that strain ACD12T shared the highest degree of 16S rRNA gene sequence similarity with Actinomadura sputi DSM 45233T (98.3 %) and Actinomadura hallensis DSM 45043T (97.8 %). All tree-making algorithms used also supported the position that strain ACD12T formed a distinct clade with its most closely related species. In addition, DNA-DNA hybridization revealed only 39.8 % relatedness with A. sputi DSM 45233T and 18.7 % relatedness with A. hallensis DSM 45043T. The combined phenotypic and genotypic data showed that the isolated strain represents a novel species of the genus Actinomadura, for which the name Actinomadura adrarensis sp. nov., is proposed, with the type strain ACD12T (=DSM 46745T = CECT 8842T).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collect... more A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collected from Béni-isguen (Mzab), Ghardaïa province, southern Algeria. The micro-organism developed small roundish sporangia on aerial mycelium that were sessile or carried by very short sporangiophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised glucose, ribose and mannose, but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The major fatty acids were iso-C16 : 0 and C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and unknown lipids. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of recognized members of the genus Streptosporangium. Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relative as Streptosporangium jomthongense BCC 53154T (98.5 % similarity), which produces single spores on aerial mycelium, but no sporangia. In hybridization experiments, the DNA-DNA relatedness values recorded between strain SG20T and S. jomthongense DSM 46822T fell well below 70 %. On the basis of phenotypic and genotypic data, strain SG20T can be distinguished as representing a novel species of the genus Streptosporangium, for which the name Streptosporangium saharense sp. nov. is proposed. The type strain is SG20T ( = DSM 46743T = CECT 8840T).
Journal of Antibiotics, 2004
Antonie van Leeuwenhoek, 2015
A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sedi... more A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36(T) was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36(T) was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37-55 °C and 7.0-9.0, respectively and the optimum NaCl concentration for growth was found to be 0-7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36(T) was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36(T) are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36(T) and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36(T) should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36(T) (=DSM 45951(T) = CECT 8579(T)).
Extremophiles, Aug 8, 2012
A halophilic actinomycete strain designated H19(T), was isolated from a Saharan soil in the Bamen... more A halophilic actinomycete strain designated H19(T), was isolated from a Saharan soil in the Bamendil region (Ouargla province, South Algeria) and was characterized taxonomically by using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain were consistent with those of members of the genus Actinopolyspora, and 16S rRNA gene sequence analysis confirmed that strain H19(T) was a novel species of the genus Actinopolyspora. DNA-DNA hybridization value between strain H19(T) and the nearest Actinopolyspora species, A. halophila, was clearly below the 70 % threshold. The genotypic and phenotypic data showed that the organism represents a novel species of the genus Actinopolyspora for which the name Actinopolyspora algeriensis sp. nov. is proposed, with the type strain H19(T) (= DSM 45476(T) = CCUG 62415(T)).
International Journal of Systematic and Evolutionary Microbiology, 2015
The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algeria... more The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produces non-motile, round to oval shaped spores, with a smooth surface, sessile or carried by short sporophores. Chemotaxonomically, the isolate 169T showed the same results as members of the genus Streptosporangium, but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169T was a member of the genus Streptosporangium. Strain 169T was most closely related to Streptosporangium jomthongense DSM 46822T (99.3 %), which is the only non-sporangia-forming species reported among the genus. However, DNA-DNA hybridization studies with this species showed 60 % similarity. Based upon genotypic and phenotypic data, a novel species, Streptosporangium algeriense sp. nov., is proposed, with 169T (=DSM 45455T =MTCC 11561T =CCUG 62974T) as the type strain.
Chemistry & biodiversity, 2017
In the present work, the Brassica rapa var. rapifera parts essential oils and their antioxidant a... more In the present work, the Brassica rapa var. rapifera parts essential oils and their antioxidant and antimicrobial activities were investigated for the first time depending on geographic origin and extraction technique. Gas-chromatography (GC) and GC/mass spectrometry (MS) analyses showed several constituents, including alcohols, aldehydes, esters, ketones, norisoprenoids, terpenic, nitrogen and sulphur compounds, totalizing 38 and 41 compounds in leaves and root essential oils, respectively. Nitrogen compounds were the main volatiles in leaves essential oils and sulphur compounds were the main volatiles in root essential oils. Qualitative and quantitative differences were found among B. rapa var. rapifera parts essential oils collected from different locations and extracted by hydrodistillation and microwave-assisted hydrodistillation techniques. Furthermore, our findings showed a high variability for both antioxidant and antimicrobial activities. The highlighted variability reflect...
African Journal of Microbiology Research, 2013
A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution... more A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution agar plating method using a chitin-vitamins B medium supplemented with different antibiotics as selective agents. This actinomycete produced antimicrobial substances and exhibited strong antifungal activity against mycotoxigenic fungi including Aspergillus carbonarius (M333) and Penicillium expansum, and also against phytopathogenic fungi such as Fusarium oxysporum f. sp. lini (Foln 3-5) and F. culmorum. In addition, the strain showed strong activity against the yeast Saccharomyces cerevisiae (ATCC 4226). On the other hand, the strain exhibited an interesting antibacterial activity against bacteria including multi-resistant Klebsiella pneumoniae (E40). The highest antimicrobial activities were obtained on ISP2 medium. The n-butanol extract contained three bioactive spots detected on thin layer chromatography (TLC) plates. Strain PP14 was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Streptomyces. The 16S rRNA gene sequence similarities showed that strain PP14 is closely associated with members of the Streptomyces violaceoruber species group (S. violaceoruber, Streptomyces violaceolatus, Streptomyces tricolor, Streptomyces humiferus, Streptomyces coelescens and Streptomyces anthocyanicus). Furthermore, the comparison of physiological characteristics of strain PP14 with the S. violaceoruber species group showed significant differences. Our results showed that strain PP14 represents a distinct phyletic line suggesting a new genomic species.
Chemistry & Biodiversity, 2016
The impact of phenological stages (vegetative, flowering and fruiting stages) on chemical composi... more The impact of phenological stages (vegetative, flowering and fruiting stages) on chemical composition, antioxidant, and antimicrobial activities of Tetraclinis articulata (Vahl) Mast. parts essential oils were investigated for the first time. GC and GC/MS analyses pointed to a quantitative variability of components; terpene hydrocarbons derivatives, represented by α-pinene (16.38 ± 0.19 - 31.78 ± 0.35%) and limonene (3.41 ± 0.07 - 9.49 ± 0.14%) as major components, predominate at the vegetative stage, whereas oxygenated derivatives, represented by camphor (16.11 ± 0.23 - 26.17 ± 0.29%) and bornyl acetate (15.21 ± 0.21 - 27.33 ± 0.33%) as major components, predominate at the fruiting stage. Furthermore, our findings showed that the plant parts collected at the fruiting stage possess the highest antioxidant activity and the best antimicrobial activity against the tested microorganisms, than plant parts collected at the vegetative and flowering stages. This highlighted variability reflects the high impact of phenological cycle on chemical composition and biological activities, which led to conclude that we should select essential oils to be investigated carefully depending on phenological stage, in order to have the highest effectiveness of essential oil in terms of biological activities for human health purposes.
Antonie van Leeuwenhoek, 2016
A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample ... more A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3(T). The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C17:1ω9c (38.6%) and C17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3(T) shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632(T) (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177(T) (99.2%) and P. parontospora subsp. antibiotica DSM 43869(T) (99.0%). DNA-DNA hybridization values between strain PM3(T) and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3(T) represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3(T) (=DSM 46752(T) = CECT 9047(T)).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterial strain, designated MB27T, was isolated from a Saharan soil sample collect... more A novel actinobacterial strain, designated MB27T, was isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria). Strain MB27T was characterized following a polyphasic taxonomic approach. This strain produced a branched and fragmented substrate mycelium, which was found to have a yellowish orange colour. A white scanty aerial mycelium was produced on most media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB27T belongs to the family Pseudonocardiaceae and is closely related to the genus Saccharothrix. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose, ribose and small amounts of mannose and rhamnose. The detected phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Mycolic acids were not detected while the predominant fatty acid was iso-branched hexadecanoate (iso-C16 : 0). The major menaquinone was MK-9(H4). Results of 16S rRNA gene sequence comparisons revealed that strain MB27T shairs the highest degree of similarity with Saccharothrix ecbatanensis DSM 45486T (99.8%), Saccharothrix hoggarensis DSM 45457T (99.3 %), Saccharothrix longispora DSM 43749T (98.6 %) and Saccharothrix yanglingensis DSM 45665T (98.6 %). However, it exhibited only 11-42 % DNA-DNA relatedness to the neighbouring Saccharothrixspecies. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB27T is shown to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix isguenensis sp. nov. (type strain MB27T=DSM 46885T=CECT 9045T) is proposed.
Biocontrol Science and Technology, 2016
Canadian Journal of Microbiology, 1987
Abstract.-This study aims to demonstrate the therapeutic advantage of traditional date vinegar. W... more Abstract.-This study aims to demonstrate the therapeutic advantage of traditional date vinegar. We carried out an ethno-pharmacological survey on the therapeutic use of date vinegar by the population of Ghardaïa. After we verified the quality of two vinegars made from Deglet-Nour and Temjouhart date varieties, we tested their antimicrobial activity on Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. These microorganisms are involved in skin infections treated with date vinegar. The ethno-pharmacological survey showed that the traditional date vinegar is used in the treatment of 30 afflictions. The treatment seems to be effective (75% of only positive effects compared to 14% of positive and also adverse effects). The antimicrobial activity was present, in spite of the weak concentration of acetic acid in the tested vinegars. The highest antimicrobial effect was observed on S. aureus. The inhibition diameter of this microorganism was 49 mm in Deglet-Nour vinegar and 33 mm in Temjouhart vinegar. In conclusion, date vinegar is used by the population of Ghardaïa to treat various illnesses and it was proven that it is effective in the treatment of skin infections caused by microorganisms mentioned previously. Résumé.-Le but de cette étude est de démontrer l'intérêt thérapeutique du vinaigre traditionnel de dattes. Nous avons mené une étude ethno-pharmacologique sur le traitement par le vinaigre de deux variétés de dattes auprès de la population de Ghardaïa. En suite, nous avons vérifié la qualité de deux vinaigres fabriqués à partir des dattes de Deglet-Nour et de Temjouhart, puis testé leur activité antimicrobienne contre Staphylococcus aureus, Pseudomonas aeruginosa et Candida albicans. Ces germes sont impliqués dans les infections de la peau traitées par le vinaigre de dattes. Les résultats ont montré que le vinaigre traditionnel de dattes est utilisé dans le traitement de 30 affections. Le traitement semble être efficace (75% de résultats positif et sans aucun effet indésirable par rapport à 14% de résultats positifs mais avec des effets indésirables). L'activité antimicrobienne était présente, malgré la concentration faible en acide acétique dans les deux vinaigres. L'effet antimicrobien le plus fort a été observé contre S. aureus avec un diamètre d'inhibition de 49 mm chez le vinaigre de Deglet-Nour et de 33 mm chez le vinaigre de Temjouhart. On a conclu que le vinaigre de dattes est utilisé par la population de Ghardaïa pour traiter diverses maladies et il s'est avéré efficace dans le traitement des infections de la peau causées par les germes précités. Mots clés: vinaigre de dattes, ethno-pharmacologie, activité antimicrobienne, infections de la peau, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans. Ethno-pharmocological use and antimicrobial activity of traditional date vinegar of Ghardaïa
Journal of Plant Diseases and Protection, 2015
International Journal of Systematic and Evolutionary Microbiology, 2016
Throughout our investigations for new actinobacterial taxa, a new actinobacterial strain, designa... more Throughout our investigations for new actinobacterial taxa, a new actinobacterial strain, designated MB20T, was isolated from a Saharan soil sample, collected in Mzab region (Ghardaïa province, South Algeria). In order to reveal its taxonomic position, the novel strain was characterized following a polyphasic taxonomic approach. It was noticed that strain MB20T produce white branched and fragmented substrate mycelium with no aerial mycelium on the most tested media. Chemotaxonomic and phylogenetic studies clearly demonstrated that the strain MB20T belonged to the family Pseudonocardiaceae and was closely related to the genus Actinophytocola. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose and ribose. The diagnostic phospholipid was phosphatidylethanolamine. Mycolic acids were not detected while the predominant fatty acid was found to be iso-branched hexadecanoate (iso-C16 : 0). The major menaquinone was MK-9(H4). Results of the 16S rRNA gene sequence comparison revealed that strain MB20T shared the highest degree of similarity with Actinophytocola gilvus DSM 45828T (98.5 %), Actinophytocola corallina DSM 45659T (98.0 %) and Actinophytocola timorensis DSM 45660T (97.5 %). However, DNA-DNA hybridization studies showed only 32.9 % similarity with A. timorensis, 23.7 % similarity with A. gilvus and 17.9 % similarity with A. corallina. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB20T revealed to be a novel species of the genus Actinophytocola, for which the name Actinophytocola algeriensis sp. nov. (type strain MB20T =DSM 46746T = CECT 8960T) is proposed.
International Journal of Systematic and Evolutionary Microbiology, 2016
The taxonomic position of a novel actinobacterium, strain SG1T, isolated from a Saharan soil samp... more The taxonomic position of a novel actinobacterium, strain SG1T, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria) was established by using a polyphasic approach. The microorganism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones was MK-9(H2) and MK-9(H4). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C17 : 1 ω8c, iso-C16 : 0, 10-methyl C17 : 0, C18 : 1 ω9c and C17 : 0. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was most closely related to 'Streptosporangium subfuscum' DSM 46724T (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181T (98.7 %), Streptosporangium fragile DSM 43847T (98.6 %) and Streptosporangium sandarakinum DSM 45763T (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1T formed a cluster with its closest relative 'S. subfuscum' DSM 46724T. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1T could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1T should be classified as the type strain of a novel species in the genus Streptosporangium, for which the name Streptosporangium becharense sp. nov., is proposed. The type strain is SG1T (=DSM 46887T =CECT 8961T).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collec... more A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collected from Adrar province, South of Algeria. A polyphasic study was carried out to establish the taxonomic position of this strain. Strain ACD12T was observed to form extensively branched substrate mycelia. Aerial mycelium was absent or weakly produced on all tested media, while spore chains are short with hooked and irregular spirals forms (2-3 turns). The dominant diaminopimelic acid isomer in the cell wall was meso-diaminopimelic acid. Glucose, ribose, galactose, mannose and madurose occur in whole-cell hydrolysates. The major phospholipid was diphosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H6). The fatty acid profile was characterized by the presence of C16:0, C17:0, C15:0, C18:0, C18:1 cis9 and iso-C16:0. Results of 16S rRNA gene sequence comparison revealed that strain ACD12T shared the highest degree of 16S rRNA gene sequence similarity with Actinomadura sputi DSM 45233T (98.3 %) and Actinomadura hallensis DSM 45043T (97.8 %). All tree-making algorithms used also supported the position that strain ACD12T formed a distinct clade with its most closely related species. In addition, DNA-DNA hybridization revealed only 39.8 % relatedness with A. sputi DSM 45233T and 18.7 % relatedness with A. hallensis DSM 45043T. The combined phenotypic and genotypic data showed that the isolated strain represents a novel species of the genus Actinomadura, for which the name Actinomadura adrarensis sp. nov., is proposed, with the type strain ACD12T (=DSM 46745T = CECT 8842T).
International Journal of Systematic and Evolutionary Microbiology, 2016
A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collect... more A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collected from Béni-isguen (Mzab), Ghardaïa province, southern Algeria. The micro-organism developed small roundish sporangia on aerial mycelium that were sessile or carried by very short sporangiophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised glucose, ribose and mannose, but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The major fatty acids were iso-C16 : 0 and C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and unknown lipids. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of recognized members of the genus Streptosporangium. Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relative as Streptosporangium jomthongense BCC 53154T (98.5 % similarity), which produces single spores on aerial mycelium, but no sporangia. In hybridization experiments, the DNA-DNA relatedness values recorded between strain SG20T and S. jomthongense DSM 46822T fell well below 70 %. On the basis of phenotypic and genotypic data, strain SG20T can be distinguished as representing a novel species of the genus Streptosporangium, for which the name Streptosporangium saharense sp. nov. is proposed. The type strain is SG20T ( = DSM 46743T = CECT 8840T).
Journal of Antibiotics, 2004
Antonie van Leeuwenhoek, 2015
A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sedi... more A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36(T) was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36(T) was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37-55 °C and 7.0-9.0, respectively and the optimum NaCl concentration for growth was found to be 0-7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36(T) was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36(T) are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36(T) and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36(T) should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36(T) (=DSM 45951(T) = CECT 8579(T)).
Extremophiles, Aug 8, 2012
A halophilic actinomycete strain designated H19(T), was isolated from a Saharan soil in the Bamen... more A halophilic actinomycete strain designated H19(T), was isolated from a Saharan soil in the Bamendil region (Ouargla province, South Algeria) and was characterized taxonomically by using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain were consistent with those of members of the genus Actinopolyspora, and 16S rRNA gene sequence analysis confirmed that strain H19(T) was a novel species of the genus Actinopolyspora. DNA-DNA hybridization value between strain H19(T) and the nearest Actinopolyspora species, A. halophila, was clearly below the 70 % threshold. The genotypic and phenotypic data showed that the organism represents a novel species of the genus Actinopolyspora for which the name Actinopolyspora algeriensis sp. nov. is proposed, with the type strain H19(T) (= DSM 45476(T) = CCUG 62415(T)).
International Journal of Systematic and Evolutionary Microbiology, 2015
The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algeria... more The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produces non-motile, round to oval shaped spores, with a smooth surface, sessile or carried by short sporophores. Chemotaxonomically, the isolate 169T showed the same results as members of the genus Streptosporangium, but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169T was a member of the genus Streptosporangium. Strain 169T was most closely related to Streptosporangium jomthongense DSM 46822T (99.3 %), which is the only non-sporangia-forming species reported among the genus. However, DNA-DNA hybridization studies with this species showed 60 % similarity. Based upon genotypic and phenotypic data, a novel species, Streptosporangium algeriense sp. nov., is proposed, with 169T (=DSM 45455T =MTCC 11561T =CCUG 62974T) as the type strain.