Natacha Teissier - Academia.edu (original) (raw)

Papers by Natacha Teissier

Journal of Neuropathology & Experimental Neurology, 2014

Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public... more Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public health concern. The pathogenesis of cerebral lesions remains unclear. We report the neuropathologic substrates, the immune response, and the cellular targets of CMV in 16 infected human fetal brains aged 23 to 28.5 gestational weeks. Nine cases were microcephalic, 10 had extensive cortical lesions, 8 had hippocampal abnormalities, and 5 cases showed infection of the olfactory bulb. The density of CMV-immunolabeled cells correlated with the presence of microcephaly and the extent of brain abnormalities. Innate and adaptive immune responses were present but did not react against all CMV-infected cells. Cytomegalovirus infected all cell types but showed higher tropism for stem cells/radial glial cells. The results indicate that 2 main factors influence the neuropathologic outcome at this stage: the density of CMV-positive cells and the tropism of CMV for stem/progenitor cells. This suggests that the large spectrum of CMV-induced brain abnormalities is caused not only by tissue destruction but also by the particular vulnerability of stem cells during early brain development. Florid infection of the hippocampus and the olfactory bulb may expose these patients to the risk of neurocognitive and sensorineural handicap even in cases of infection at late stages of gestation.

Journal of Neuropathology & Experimental Neurology, 2014

Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public... more Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public health concern. The pathogenesis of cerebral lesions remains unclear. We report the neuropathologic substrates, the immune response, and the cellular targets of CMV in 16 infected human fetal brains aged 23 to 28.5 gestational weeks. Nine cases were microcephalic, 10 had extensive cortical lesions, 8 had hippocampal abnormalities, and 5 cases showed infection of the olfactory bulb. The density of CMV-immunolabeled cells correlated with the presence of microcephaly and the extent of brain abnormalities. Innate and adaptive immune responses were present but did not react against all CMV-infected cells. Cytomegalovirus infected all cell types but showed higher tropism for stem cells/radial glial cells. The results indicate that 2 main factors influence the neuropathologic outcome at this stage: the density of CMV-positive cells and the tropism of CMV for stem/ progenitor cells. This suggests that the large spectrum of CMV-induced brain abnormalities is caused not only by tissue destruction but also by the particular vulnerability of stem cells during early brain development. Florid infection of the hippocampus and the olfactory bulb may expose these patients to the risk of neurocognitive and sensorineural handicap even in cases of infection at late stages of gestation.

Acta Neuropathologica, 2011

Congenital cytomegalovirus (CMV) infection is the leading cause of non-hereditary congenital sens... more Congenital cytomegalovirus (CMV) infection is the leading cause of non-hereditary congenital sensorineural hearing loss (SNHL). The natural course and the pathophysiology of inner ear lesions during human fetal CMV infection have not yet been reported. Inner ear lesions were investigated in six CMV-infected fetuses aged 19-35 postconceptional weeks and correlated with central nervous system (CNS) lesions. All the fetuses had high viral loads in the amniotic fluid and severe visceral and CNS lesions visible by ultrasound. Diffuse lesions consisting of both cytomegalic cells containing inclusion bodies and inflammation were found within all studied structures including the inner ear, brain, other organs, and placenta, suggesting hematogenous dissemination. Cochlear infection was consistently present and predominated in the stria vascularis (5/6), whereas the supporting cells in the organ of Corti were less often involved (2/6). Vestibular infection, found in 4/6 cases, was florid; the Electronic supplementary material The online version of this article (non-sensory epithelia, including the dark cells, were extensively infected. The endolymphatic sac was infected in 1 of 3 cases. The severity of inner ear infection was correlated with the CNS lesions, confirming the neurotropism of CMV. This study documenting infection of the structures involved in endolymph secretion and potassium homeostasis in fetuses with high amniotic fluid viral loads suggests that potassium dysregulation in the endolymphatic compartment of the inner ear may lead to secondary degeneration of the sensory structures. In addition, the occurrence of SNHL depends on the intensity and duration of the viral infection and inflammation.

Head & Neck

Localized pediatric parameningeal sarcomas are usually treated exclusively by chemotherapy and ra... more Localized pediatric parameningeal sarcomas are usually treated exclusively by chemotherapy and radiotherapy. In this location, surgery is complicated but sometimes attempted to improve local control. A retrospective bicentric study was conducted to examine its place with particular reference to acute and long-term morbidity. Fifteen patients under the age of 20 years with parameningeal sarcoma underwent surgery between 2000 and 2007. Surgery was performed for 8 primary sarcomas and 7 radiation-induced sarcomas, mainly in infratemporal fossa. Three children had intracranial extension, 3 had metastases, and 1 had both. Median follow-up was 46 months (16-154 months). Five children experienced local relapse. Eight presented sequelae. Eleven children are alive with no evidence of disease, and 4 died. Skull base surgery should be considered as a possible treatment in pediatric parameningeal sarcomas. Surgery is the only option in radiation-induced sarcoma. Larger studies are necessary to ...

International Journal of Pediatric Otorhinolaryngology, 2010

Sialoblastoma is a very rare congenital salivary gland tumor. No consensus has been reached conce... more Sialoblastoma is a very rare congenital salivary gland tumor. No consensus has been reached concerning the treatment of this tumor due to its rarity. The treatment of reference is surgery, which can be mutilating, in the case of a locally invasive tumor. The treatment of metastatic disease is also controversial. The authors report a new case of a 6-year-old girl

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2013

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

EMC - Otorrinolaringología, 2008

ABSTRACT Las malformaciones del oído externo y medio son relativamente infrecuentes y en la mayor... more ABSTRACT Las malformaciones del oído externo y medio son relativamente infrecuentes y en la mayoría de los casos aparecen de forma aislada y unilateral. Sin embargo, pueden integrarse en asociaciones sindrómicas que conviene detectar para buscar malformaciones asociadas y aportar un posible consejo genético. En la mayoría de las ocasiones es necesario un tratamiento multidisciplinario en el que participen pediatras, genetistas, audiólogos y cirujanos, para proponer a los padres un programa terapéutico adecuado.

EMC - Oto-rhino-laryngologie, 2008

But de la présentation L’infection congénitale par le cytomégalovirus (CMV) est responsable de 20... more But de la présentation L’infection congénitale par le cytomégalovirus (CMV) est responsable de 20 à 30 % des surdités neurosensorielles chez l’enfant. Certaines nécessiteront une implantation cochléaire. Le but de cette étude est d’évaluer les résultats des enfants implantés avec et sans anomalies cérébrales dans un contexte d’infection congénitale par le CMV. Matériel et méthodes Parmi les 333 patients implantés entre 1998 et 2013, vingt-six patients présentaient une surdité profonde bilatérale dans un contexte d’infection congénitale par le CMV. Le diagnostic a été confirmé dans 16 cas par PCR, 7 patients présentaient des images cérébrales évocatrices à l’IRM et 3 patients avaient une histoire compatible avec une infection congénitale par le CMV. Pour chaque enfant, le niveau de langage et les index APCEI (dont le score global est réparti en 5 classes K de 1 à 5 des moins bons aux meilleurs résultats) ont été évalués. Résultats L’âge moyen à l’implantation était de 3,7 ans (1,3 à ...

International Journal of Pediatric Otorhinolaryngology, 2015

To investigate predictive factors of surgical management of subperiosteal orbital abscess in chil... more To investigate predictive factors of surgical management of subperiosteal orbital abscess in children. A retrospective monocentric study was conducted between 2000 and 2011 with children hospitalized for acute pediatric orbital cellulitis (APOC). Clinical, biological and radiological data as well as medical and surgical management were collected and analyzed. All patients received intravenous antibiotics and underwent a CT-scan. Orbit and subperiosteal intraorbital abscess dimensions were measured on axial and coronal planes and the abscess volume was calculated using a spheroid model. Eighty-three children with APOC (mean age: 4.5 years) were included, 53 were boys (63.9%). Thirty-two children (38.6%) presented with a subperiosteal orbital abscess. Mean abscess volume was 570mm(3) and mean exophthalmos was 4.7mm. Twenty patients were treated surgically, 11 of which by an endoscopic approach. A positive correlation was observed between the volume of the abscess or exophthalmos and surgical drainage: 57.9% of patients underwent surgery when exophthalmos was >4mm, 29.4% between 2 and 4mm, and none when <2mm. All patients with an abscess volume >500mm(3) or >5% of orbital volume were operated on whereas only 30% or 39% of patients, respectively, in case of smaller volumes (P<0.05). Surgery for subperiosteal orbital abscess is usually performed in case of visual complications or unfavorable medical outcome. The importance of the exophthalmos and the volume of the abscess measured on the CT-scan are predictive factors of surgery in children with subperiosteal orbital abscess without visual complications.

Otology & Neurotology, 2014

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

International Journal of Pediatric Otorhinolaryngology, 2010

EMC - Otorinolaringoiatria, 2008

ABSTRACT Le deformità dell’orecchio esterno e medio sono relativamente rare, abitualmente isolate... more ABSTRACT Le deformità dell’orecchio esterno e medio sono relativamente rare, abitualmente isolate e monolaterali. Possono, tuttavia, integrarsi in alcune associazioni sindromiche che è opportuno individuare in modo da ricercare malformazioni associate e in modo da fornire un’eventuale consulenza genetica. È necessaria, il più delle volte, una gestione multidisciplinare che associa pediatri, genetisti, audiologi e chirurghi in modo da proporre ai genitori un programma terapeutico adatto.

Journal de Radiologie, 2007

Journal of Thoracic and Cardiovascular Surgery, 2006

Figure 1. The hypoplasia started at the cricoid level and extended to the carina.

Journal de Radiologie, 2009

Pediatric Radiology, 2010

Journal of Neuropathology & Experimental Neurology, 2014

Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public... more Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public health concern. The pathogenesis of cerebral lesions remains unclear. We report the neuropathologic substrates, the immune response, and the cellular targets of CMV in 16 infected human fetal brains aged 23 to 28.5 gestational weeks. Nine cases were microcephalic, 10 had extensive cortical lesions, 8 had hippocampal abnormalities, and 5 cases showed infection of the olfactory bulb. The density of CMV-immunolabeled cells correlated with the presence of microcephaly and the extent of brain abnormalities. Innate and adaptive immune responses were present but did not react against all CMV-infected cells. Cytomegalovirus infected all cell types but showed higher tropism for stem cells/radial glial cells. The results indicate that 2 main factors influence the neuropathologic outcome at this stage: the density of CMV-positive cells and the tropism of CMV for stem/progenitor cells. This suggests that the large spectrum of CMV-induced brain abnormalities is caused not only by tissue destruction but also by the particular vulnerability of stem cells during early brain development. Florid infection of the hippocampus and the olfactory bulb may expose these patients to the risk of neurocognitive and sensorineural handicap even in cases of infection at late stages of gestation.

Journal of Neuropathology & Experimental Neurology, 2014

Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public... more Neurologic morbidity associated with congenital cytomegalovirus (CMV) infection is a major public health concern. The pathogenesis of cerebral lesions remains unclear. We report the neuropathologic substrates, the immune response, and the cellular targets of CMV in 16 infected human fetal brains aged 23 to 28.5 gestational weeks. Nine cases were microcephalic, 10 had extensive cortical lesions, 8 had hippocampal abnormalities, and 5 cases showed infection of the olfactory bulb. The density of CMV-immunolabeled cells correlated with the presence of microcephaly and the extent of brain abnormalities. Innate and adaptive immune responses were present but did not react against all CMV-infected cells. Cytomegalovirus infected all cell types but showed higher tropism for stem cells/radial glial cells. The results indicate that 2 main factors influence the neuropathologic outcome at this stage: the density of CMV-positive cells and the tropism of CMV for stem/ progenitor cells. This suggests that the large spectrum of CMV-induced brain abnormalities is caused not only by tissue destruction but also by the particular vulnerability of stem cells during early brain development. Florid infection of the hippocampus and the olfactory bulb may expose these patients to the risk of neurocognitive and sensorineural handicap even in cases of infection at late stages of gestation.

Acta Neuropathologica, 2011

Congenital cytomegalovirus (CMV) infection is the leading cause of non-hereditary congenital sens... more Congenital cytomegalovirus (CMV) infection is the leading cause of non-hereditary congenital sensorineural hearing loss (SNHL). The natural course and the pathophysiology of inner ear lesions during human fetal CMV infection have not yet been reported. Inner ear lesions were investigated in six CMV-infected fetuses aged 19-35 postconceptional weeks and correlated with central nervous system (CNS) lesions. All the fetuses had high viral loads in the amniotic fluid and severe visceral and CNS lesions visible by ultrasound. Diffuse lesions consisting of both cytomegalic cells containing inclusion bodies and inflammation were found within all studied structures including the inner ear, brain, other organs, and placenta, suggesting hematogenous dissemination. Cochlear infection was consistently present and predominated in the stria vascularis (5/6), whereas the supporting cells in the organ of Corti were less often involved (2/6). Vestibular infection, found in 4/6 cases, was florid; the Electronic supplementary material The online version of this article (non-sensory epithelia, including the dark cells, were extensively infected. The endolymphatic sac was infected in 1 of 3 cases. The severity of inner ear infection was correlated with the CNS lesions, confirming the neurotropism of CMV. This study documenting infection of the structures involved in endolymph secretion and potassium homeostasis in fetuses with high amniotic fluid viral loads suggests that potassium dysregulation in the endolymphatic compartment of the inner ear may lead to secondary degeneration of the sensory structures. In addition, the occurrence of SNHL depends on the intensity and duration of the viral infection and inflammation.

Head & Neck

Localized pediatric parameningeal sarcomas are usually treated exclusively by chemotherapy and ra... more Localized pediatric parameningeal sarcomas are usually treated exclusively by chemotherapy and radiotherapy. In this location, surgery is complicated but sometimes attempted to improve local control. A retrospective bicentric study was conducted to examine its place with particular reference to acute and long-term morbidity. Fifteen patients under the age of 20 years with parameningeal sarcoma underwent surgery between 2000 and 2007. Surgery was performed for 8 primary sarcomas and 7 radiation-induced sarcomas, mainly in infratemporal fossa. Three children had intracranial extension, 3 had metastases, and 1 had both. Median follow-up was 46 months (16-154 months). Five children experienced local relapse. Eight presented sequelae. Eleven children are alive with no evidence of disease, and 4 died. Skull base surgery should be considered as a possible treatment in pediatric parameningeal sarcomas. Surgery is the only option in radiation-induced sarcoma. Larger studies are necessary to ...

International Journal of Pediatric Otorhinolaryngology, 2010

Sialoblastoma is a very rare congenital salivary gland tumor. No consensus has been reached conce... more Sialoblastoma is a very rare congenital salivary gland tumor. No consensus has been reached concerning the treatment of this tumor due to its rarity. The treatment of reference is surgery, which can be mutilating, in the case of a locally invasive tumor. The treatment of metastatic disease is also controversial. The authors report a new case of a 6-year-old girl

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2013

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

EMC - Otorrinolaringología, 2008

ABSTRACT Las malformaciones del oído externo y medio son relativamente infrecuentes y en la mayor... more ABSTRACT Las malformaciones del oído externo y medio son relativamente infrecuentes y en la mayoría de los casos aparecen de forma aislada y unilateral. Sin embargo, pueden integrarse en asociaciones sindrómicas que conviene detectar para buscar malformaciones asociadas y aportar un posible consejo genético. En la mayoría de las ocasiones es necesario un tratamiento multidisciplinario en el que participen pediatras, genetistas, audiólogos y cirujanos, para proponer a los padres un programa terapéutico adecuado.

EMC - Oto-rhino-laryngologie, 2008

But de la présentation L’infection congénitale par le cytomégalovirus (CMV) est responsable de 20... more But de la présentation L’infection congénitale par le cytomégalovirus (CMV) est responsable de 20 à 30 % des surdités neurosensorielles chez l’enfant. Certaines nécessiteront une implantation cochléaire. Le but de cette étude est d’évaluer les résultats des enfants implantés avec et sans anomalies cérébrales dans un contexte d’infection congénitale par le CMV. Matériel et méthodes Parmi les 333 patients implantés entre 1998 et 2013, vingt-six patients présentaient une surdité profonde bilatérale dans un contexte d’infection congénitale par le CMV. Le diagnostic a été confirmé dans 16 cas par PCR, 7 patients présentaient des images cérébrales évocatrices à l’IRM et 3 patients avaient une histoire compatible avec une infection congénitale par le CMV. Pour chaque enfant, le niveau de langage et les index APCEI (dont le score global est réparti en 5 classes K de 1 à 5 des moins bons aux meilleurs résultats) ont été évalués. Résultats L’âge moyen à l’implantation était de 3,7 ans (1,3 à ...

International Journal of Pediatric Otorhinolaryngology, 2015

To investigate predictive factors of surgical management of subperiosteal orbital abscess in chil... more To investigate predictive factors of surgical management of subperiosteal orbital abscess in children. A retrospective monocentric study was conducted between 2000 and 2011 with children hospitalized for acute pediatric orbital cellulitis (APOC). Clinical, biological and radiological data as well as medical and surgical management were collected and analyzed. All patients received intravenous antibiotics and underwent a CT-scan. Orbit and subperiosteal intraorbital abscess dimensions were measured on axial and coronal planes and the abscess volume was calculated using a spheroid model. Eighty-three children with APOC (mean age: 4.5 years) were included, 53 were boys (63.9%). Thirty-two children (38.6%) presented with a subperiosteal orbital abscess. Mean abscess volume was 570mm(3) and mean exophthalmos was 4.7mm. Twenty patients were treated surgically, 11 of which by an endoscopic approach. A positive correlation was observed between the volume of the abscess or exophthalmos and surgical drainage: 57.9% of patients underwent surgery when exophthalmos was >4mm, 29.4% between 2 and 4mm, and none when <2mm. All patients with an abscess volume >500mm(3) or >5% of orbital volume were operated on whereas only 30% or 39% of patients, respectively, in case of smaller volumes (P<0.05). Surgery for subperiosteal orbital abscess is usually performed in case of visual complications or unfavorable medical outcome. The importance of the exophthalmos and the volume of the abscess measured on the CT-scan are predictive factors of surgery in children with subperiosteal orbital abscess without visual complications.

Otology & Neurotology, 2014

Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale, 2012

International Journal of Pediatric Otorhinolaryngology, 2010

EMC - Otorinolaringoiatria, 2008

ABSTRACT Le deformità dell’orecchio esterno e medio sono relativamente rare, abitualmente isolate... more ABSTRACT Le deformità dell’orecchio esterno e medio sono relativamente rare, abitualmente isolate e monolaterali. Possono, tuttavia, integrarsi in alcune associazioni sindromiche che è opportuno individuare in modo da ricercare malformazioni associate e in modo da fornire un’eventuale consulenza genetica. È necessaria, il più delle volte, una gestione multidisciplinare che associa pediatri, genetisti, audiologi e chirurghi in modo da proporre ai genitori un programma terapeutico adatto.

Journal de Radiologie, 2007

Journal of Thoracic and Cardiovascular Surgery, 2006

Figure 1. The hypoplasia started at the cricoid level and extended to the carina.

Journal de Radiologie, 2009

Pediatric Radiology, 2010