Nathalie Verbruggen - Academia.edu (original) (raw)
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Papers by Nathalie Verbruggen
Plant and Cell Physiology, Jul 7, 2022
Plant Cell and Environment, Jul 13, 2018
The molecular analysis of metal hyperaccumulation in species such as Arabidopsis halleri offers t... more The molecular analysis of metal hyperaccumulation in species such as Arabidopsis halleri offers the chance to gain insights into metal homeostasis and into the evolution of adaptation to extreme habitats. A prerequisite of metal hyperaccumulation is metal hypertolerance. Genetic analysis of a backcross population derived from Arabidopsis lyrata × A. halleri crosses revealed three quantitative trait loci for Cd hypertolerance. A candidate gene for Cdtol2 is AhCAX1, encoding a vacuolar Ca /H antiporter. We developed a method for the transformation of vegetatively propagated A. halleri plants and generated AhCAX1-silenced lines. Upon Cd exposure, several-fold higher accumulation of reactive oxygen species (ROS) was detectable in roots of AhCAX1-silenced plants. In accordance with the dependence of Cdtol2 on external Ca concentration, this phenotype was exclusively observed in low Ca conditions. The effects of external Ca on Cd accumulation cannot explain the phenotype as they were not influenced by the genotype. Our data strongly support the hypothesis that higher expression of CAX1 in A. halleri relative to other Arabidopsis species represents a Cd hypertolerance factor. We propose a function of AhCAX1 in preventing a positive feedback loop of Cd-elicited ROS production triggering further Ca -dependent ROS accumulation.
Frontiers in Plant Science, 2013
Proceedings of the National Academy of Sciences of the United States of America, Mar 26, 2018
Journal of Hazardous Materials
Journal of Experimental Botany, Apr 6, 2015
New Biotechnology, Nov 1, 2012
Phytoremediation consists in treating environmental pollutions through the use of plants and thei... more Phytoremediation consists in treating environmental pollutions through the use of plants and their associated microbes. Phytoremediation can be used for pollutant stabilization, extraction, degradation or volatilization. Cadmium is one of the most toxic trace metallic elements for living organisms and its accumulation in the environment is recognized as a worldwide concern. Plants suitable for efficient pollutant extraction from the soil should combine different characteristics like fast growth, high biomass, high tolerance and high accumulation capacities in harvestable parts. A rare class of plants called hyperaccumulators combines extremely high tolerance degrees and foliar accumulation of trace elements. With regard to cadmium, none of the Cd hyperaccumulators identified has met the criteria for efficient phytoextraction so far. By virtue of genetic engineering it is possible to transfer genes involved in Cd tolerance or accumulation in high biomass plants. Nevertheless, the genetic determinants of Cd hyperaccumulation are far from being understood. It is thus indispensable to acquire more knowledge about these processes. Among Cd hyperaccumulators, Arabidopsis halleri (some populations can hyperaccumulate Cd) is considered as a model species for the study of metal homeostasis and detoxification. This review will summarize our knowledge about Cd tolerance and accumulation acquired in A. halleri and how this knowledge may be used in phytoextraction.
Book of abstractinfo:eu-repo/semantics/publishe
Journal of Experimental Botany, Nov 20, 2021
Plant Physiology, Dec 9, 2009
Journal of Experimental Botany, Mar 15, 2022
FEBS Letters, Sep 10, 1999
Proceedings of the National Academy of Sciences of the United States of America, Aug 6, 1996
COST 837 meeting du WG3, 2001
info:eu-repo/semantics/publishe
Archives of Physiology and Biochemistry, 2001
info:eu-repo/semantics/publishe
Book of Abstractinfo:eu-repo/semantics/publishe
Plant and Cell Physiology, Jul 7, 2022
Plant Cell and Environment, Jul 13, 2018
The molecular analysis of metal hyperaccumulation in species such as Arabidopsis halleri offers t... more The molecular analysis of metal hyperaccumulation in species such as Arabidopsis halleri offers the chance to gain insights into metal homeostasis and into the evolution of adaptation to extreme habitats. A prerequisite of metal hyperaccumulation is metal hypertolerance. Genetic analysis of a backcross population derived from Arabidopsis lyrata × A. halleri crosses revealed three quantitative trait loci for Cd hypertolerance. A candidate gene for Cdtol2 is AhCAX1, encoding a vacuolar Ca /H antiporter. We developed a method for the transformation of vegetatively propagated A. halleri plants and generated AhCAX1-silenced lines. Upon Cd exposure, several-fold higher accumulation of reactive oxygen species (ROS) was detectable in roots of AhCAX1-silenced plants. In accordance with the dependence of Cdtol2 on external Ca concentration, this phenotype was exclusively observed in low Ca conditions. The effects of external Ca on Cd accumulation cannot explain the phenotype as they were not influenced by the genotype. Our data strongly support the hypothesis that higher expression of CAX1 in A. halleri relative to other Arabidopsis species represents a Cd hypertolerance factor. We propose a function of AhCAX1 in preventing a positive feedback loop of Cd-elicited ROS production triggering further Ca -dependent ROS accumulation.
Frontiers in Plant Science, 2013
Proceedings of the National Academy of Sciences of the United States of America, Mar 26, 2018
Journal of Hazardous Materials
Journal of Experimental Botany, Apr 6, 2015
New Biotechnology, Nov 1, 2012
Phytoremediation consists in treating environmental pollutions through the use of plants and thei... more Phytoremediation consists in treating environmental pollutions through the use of plants and their associated microbes. Phytoremediation can be used for pollutant stabilization, extraction, degradation or volatilization. Cadmium is one of the most toxic trace metallic elements for living organisms and its accumulation in the environment is recognized as a worldwide concern. Plants suitable for efficient pollutant extraction from the soil should combine different characteristics like fast growth, high biomass, high tolerance and high accumulation capacities in harvestable parts. A rare class of plants called hyperaccumulators combines extremely high tolerance degrees and foliar accumulation of trace elements. With regard to cadmium, none of the Cd hyperaccumulators identified has met the criteria for efficient phytoextraction so far. By virtue of genetic engineering it is possible to transfer genes involved in Cd tolerance or accumulation in high biomass plants. Nevertheless, the genetic determinants of Cd hyperaccumulation are far from being understood. It is thus indispensable to acquire more knowledge about these processes. Among Cd hyperaccumulators, Arabidopsis halleri (some populations can hyperaccumulate Cd) is considered as a model species for the study of metal homeostasis and detoxification. This review will summarize our knowledge about Cd tolerance and accumulation acquired in A. halleri and how this knowledge may be used in phytoextraction.
Book of abstractinfo:eu-repo/semantics/publishe
Journal of Experimental Botany, Nov 20, 2021
Plant Physiology, Dec 9, 2009
Journal of Experimental Botany, Mar 15, 2022
FEBS Letters, Sep 10, 1999
Proceedings of the National Academy of Sciences of the United States of America, Aug 6, 1996
COST 837 meeting du WG3, 2001
info:eu-repo/semantics/publishe
Archives of Physiology and Biochemistry, 2001
info:eu-repo/semantics/publishe
Book of Abstractinfo:eu-repo/semantics/publishe