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Papers by Nathrada Khantavee

Research paper thumbnail of Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Veterinary Dermatology, Nov 6, 2019

Research paper thumbnail of Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis

Veterinary Dermatology, May 17, 2021

BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (... more BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. OBJECTIVES We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further diagnostic and therapeutic applications. ANIMALS Serum samples were collected from five healthy, nine Der f-allergic atopic and seven non-Der f-allergic atopic dogs identified based on an intradermal skin test. METHODS AND MATERIALS We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE-binding components in both groups of atopic dogs were analysed by mass spectrometry. RESULTS The majority of Der f-allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1-alpha (EF1-α), gelsolin-like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha-enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. CONCLUSIONS AND CLINICAL IMPORTANCE Multiple IgE-binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1-α, gelsolin-like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.

Research paper thumbnail of Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

BMC Veterinary Research, Feb 5, 2021

Research paper thumbnail of Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis

Veterinary Dermatology, 2021

BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (... more BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. OBJECTIVES We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further diagnostic and therapeutic applications. ANIMALS Serum samples were collected from five healthy, nine Der f-allergic atopic and seven non-Der f-allergic atopic dogs identified based on an intradermal skin test. METHODS AND MATERIALS We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE-binding components in both groups of atopic dogs were analysed by mass spectrometry. RESULTS The majority of Der f-allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1-alpha (EF1-α), gelsolin-like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha-enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. CONCLUSIONS AND CLINICAL IMPORTANCE Multiple IgE-binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1-α, gelsolin-like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.

Research paper thumbnail of Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

BMC Veterinary Research, 2021

Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE ser... more Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy ( n = 33) and atopic dogs (AD) ( n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p...

Research paper thumbnail of Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Veterinary Dermatology, 2019

BackgroundElevated immunoglobulin E (IgE) levels to Malassezia or Staphylococcus species in human... more BackgroundElevated immunoglobulin E (IgE) levels to Malassezia or Staphylococcus species in human atopic dermatitis are related to the skin severity index; a similar association has not been reported in atopic dogs.ObjectivesTo investigate serum levels of allergen‐specific IgE, total specific IgG and IgG subclasses (IgG1 and IgG2) for M. pachydermatis and S. pseudintermedius, and to correlate them with the severity of dermatitis in dogs.AnimalsSerum samples were collected from dogs categorized by age and disease status. Groups 1 and 2: <3‐year‐old healthy (n = 9) and atopic dogs (n = 9), respectively; and groups 3 and 4: ≥3‐year‐old healthy (n = 11) and atopic dogs (n = 14), respectively.Methods and materialsAntibody levels were measured by ELISA. The Canine Atopic Dermatitis Lesion Index (CADLI) was analyzed in relation to antibody levels.ResultsSpecific IgE and total IgG against M. pachydermatis and S. pseudintermedius were significantly increased in atopic dogs of all ages. Al...

Research paper thumbnail of Correlation of allergen specific immunoglobulin to house dust mites and commensal microbes in atopic dogs and identification of major allergen in dermatophagoides farinae

To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) ... more To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus pseudintermedius �bacteria. The results was found that high IgE levels to house dust mites could be used to differentiate atopic dog with allergy to house dust mites and atopic dogs without allergy to house dust mites. Moreover, IgG1 levels could also use to differentiate house dust mite allergy and non-house dust mite al...

Research paper thumbnail of Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Veterinary Dermatology, Nov 6, 2019

Research paper thumbnail of Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis

Veterinary Dermatology, May 17, 2021

BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (... more BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. OBJECTIVES We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further diagnostic and therapeutic applications. ANIMALS Serum samples were collected from five healthy, nine Der f-allergic atopic and seven non-Der f-allergic atopic dogs identified based on an intradermal skin test. METHODS AND MATERIALS We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE-binding components in both groups of atopic dogs were analysed by mass spectrometry. RESULTS The majority of Der f-allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1-alpha (EF1-α), gelsolin-like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha-enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. CONCLUSIONS AND CLINICAL IMPORTANCE Multiple IgE-binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1-α, gelsolin-like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.

Research paper thumbnail of Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

BMC Veterinary Research, Feb 5, 2021

Research paper thumbnail of Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis

Veterinary Dermatology, 2021

BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (... more BACKGROUND Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. OBJECTIVES We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further diagnostic and therapeutic applications. ANIMALS Serum samples were collected from five healthy, nine Der f-allergic atopic and seven non-Der f-allergic atopic dogs identified based on an intradermal skin test. METHODS AND MATERIALS We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE-binding components in both groups of atopic dogs were analysed by mass spectrometry. RESULTS The majority of Der f-allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1-alpha (EF1-α), gelsolin-like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha-enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. CONCLUSIONS AND CLINICAL IMPORTANCE Multiple IgE-binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1-α, gelsolin-like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.

Research paper thumbnail of Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

BMC Veterinary Research, 2021

Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE ser... more Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy ( n = 33) and atopic dogs (AD) ( n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p...

Research paper thumbnail of Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores

Veterinary Dermatology, 2019

BackgroundElevated immunoglobulin E (IgE) levels to Malassezia or Staphylococcus species in human... more BackgroundElevated immunoglobulin E (IgE) levels to Malassezia or Staphylococcus species in human atopic dermatitis are related to the skin severity index; a similar association has not been reported in atopic dogs.ObjectivesTo investigate serum levels of allergen‐specific IgE, total specific IgG and IgG subclasses (IgG1 and IgG2) for M. pachydermatis and S. pseudintermedius, and to correlate them with the severity of dermatitis in dogs.AnimalsSerum samples were collected from dogs categorized by age and disease status. Groups 1 and 2: <3‐year‐old healthy (n = 9) and atopic dogs (n = 9), respectively; and groups 3 and 4: ≥3‐year‐old healthy (n = 11) and atopic dogs (n = 14), respectively.Methods and materialsAntibody levels were measured by ELISA. The Canine Atopic Dermatitis Lesion Index (CADLI) was analyzed in relation to antibody levels.ResultsSpecific IgE and total IgG against M. pachydermatis and S. pseudintermedius were significantly increased in atopic dogs of all ages. Al...

Research paper thumbnail of Correlation of allergen specific immunoglobulin to house dust mites and commensal microbes in atopic dogs and identification of major allergen in dermatophagoides farinae

To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) ... more To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus pseudintermedius �bacteria. The results was found that high IgE levels to house dust mites could be used to differentiate atopic dog with allergy to house dust mites and atopic dogs without allergy to house dust mites. Moreover, IgG1 levels could also use to differentiate house dust mite allergy and non-house dust mite al...