Natividad Sebastià - Academia.edu (original) (raw)
Papers by Natividad Sebastià
According to the International Agency for Research on Cancer, smoking tobacco is a major cause of... more According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n = 48) and non-smokers (n = 53), aged from 18 to 49 years, was studied using SCE levels in peripheral lymphocytes. Heavy smoking (⩾10 cigarettes per day) increased significantly the SCE frequency and the HFC parameters. Neither age nor sex significantly influenced the frequencies in the groups studied.
Radiation Measurements, 2011
Many natural substances have been studied in recent past to be used as radioprotectors to mitigat... more Many natural substances have been studied in recent past to be used as radioprotectors to mitigate ionizing radiation-induced damage in mammalian systems due to its effectiveness given both pre- and post-irradiation and for long time with out drug-related toxicity. Curcumin and trans-resveratrol are both natural occurring polyphenols, obtained from the root of Curcuma longa and from grapes and other berries,
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](Nutrición hospitalaria, 2014
dietotherapeutical softwares are now a basic tool in the dietary management of patients, either f... more dietotherapeutical softwares are now a basic tool in the dietary management of patients, either from a physiological point of view and / or pathological. New technologies and research in this regard, have favored the emergence of new applications for the dietary and nutritional management that facilitate the management of the dietotherapeutical company. To comparatively study the main dietotherapeutical applications on the market to give criteria to the professional users of diet and nutrition in the selection of one of the main tools for these. Dietopro.com is, from our point of view, one of the most comprehensive management of patients dietotherapeutical applications. Based on the need of the user, it has different dietary sofwares choice.We conclude that there is no better or worse than another application, but applications roughly adapted to the needs of professionals.
Journal of the Science of Food and Agriculture, 2012
Resveratrol is a polyphenol with health properties being mainly present in the skins of several f... more Resveratrol is a polyphenol with health properties being mainly present in the skins of several foods. However, any study has been carried out to analyze the presence of this stilbene in the plum fruit from the genus Prunus in European and Japanese cultivars. The analysis of resveratrol from the skin in different cultivars of plums from Spanish markets with liquid chromatography coupled to ultraviolet (LC-UV) detector with subsequent confirmation by LC-MS/MS has been demonstrated that contents of this compound in plums ranged from 0.1 to 6.2 µg/g. Values of resveratrol in European plum cultivars is higher than in Japanese cultivars.
Journal of Agricultural and Food Chemistry, 2010
A method based on matrix solid phase dispersion extraction was applied to determine aflatoxins B(... more A method based on matrix solid phase dispersion extraction was applied to determine aflatoxins B(1), B(2), G(1), and G(2) from tigernuts and tigernut beverages. Recoveries of each aflatoxin from tigernut (spiked at 10 microg/kg level) and from tigernut beverages (spiked at 10 microg/L level) ranged from 72.3 to 82.1% and from 74.0 to 86.3%, respectively. The limits of quantification ranged from 0.21 to 1.49 microg/kg (for tigernuts) and from 0.13 to 0.57 microg/L (for tigernut beverages) studied using liquid chromatography with fluorescence detection. The proposed extraction method followed by liquid chromatography-fluorescence detection determination was applied to 37 and 25 samples of tigernuts and tigernut beverages, respectively, 3 positives being found in each category.
International Journal of Radiation Biology, 2013
Purpose : To survey the possible presence of chromosomal damage and internal contamination in a g... more Purpose : To survey the possible presence of chromosomal damage and internal contamination in a group of Ukrainian children and adolescents, 20 years after the Chernobyl accident at the Nuclear Power Plant. Materials and methods: Cytogenetical procedures were performed according to dicentric assay in 55 Ukrainian children and adolescents (29 boys and 26 girls), living near Chernobyl. In addition, a whole body detector and urinalysis were used to detect internal contamination. Results : 36 dicentrics were found in a total of 53,477 metaphases scored in these children, which refl ected a frequency of dicentrics below the background level. On the other hand, internal contamination was not detected in any subject studied. Conclusions : Since the estimated absorbed dose is below the detection limit, according to both biological and physical dosimetry, radiation overexposure during the last 3 -5 years has not been detected in the considered subjects.
Food Chemistry, 2011
A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ES... more A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB 1 , AFB 2 , AFG 1 and AFG 2 ) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C 18 , while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010. A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB 1 , AFB 2 and AFG 2 .
Food and Chemical Toxicology, 2014
According to the International Agency for Research on Cancer, smoking tobacco is a major cause of... more According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n = 48) and non-smokers (n = 53), aged from 18 to 49 years, was studied using SCE levels in peripheral lymphocytes. Heavy smoking (P10 cigarettes per day) increased significantly the SCE frequency and the HFC parameters. Neither age nor sex significantly influenced the frequencies in the groups studied.
Food and Chemical Toxicology, 2013
Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This... more Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This compound has shown antioxidant, anti-inflammatory, immunostimulant or anti-carcinogenic properties. Our aim was to evaluate the radioprotective efficacy, in vitro, of trans-resveratrol against radiationinduced chromosomal damage and to study the genotoxicity and cytotoxicity of this polyphenol in cell cultures without irradiation. The study was carried out by the pre-treatment of human lymphocytes at concentrations from 0 to 219 lM of trans-resveratrol. The results showed that all concentrations tested reduced radiation-induced chromosomal damage compared with cells with any treatment. Maximum damage protection was observed at the concentration of 2.19 lM. Concerning genotoxic results, all tested trans-resveratrol concentrations increased the sister chromatid exchange (SCE) index compared with no trans-resveratrol treatment. Cytotoxic indexes (Mitotic and Proliferation Index) showed that the lowest concentrations could enhance the cell proliferation rates and the highest ones could negatively affect to human peripheral lymphocytes growth.
Food and Chemical Toxicology, 2012
We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocyt... more We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000 lg ml À1 ). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.
Food and Chemical Toxicology, 2012
Curcumin has shown a wide range of properties such as anti-inflammatory and anti-carcinogenic pro... more Curcumin has shown a wide range of properties such as anti-inflammatory and anti-carcinogenic properties. Many of these effects, mainly the anti-carcinogenic effect, could be linked to its anti-oxidant effects. Nevertheless, some studies suggest that this natural compound possesses both pro-and antioxidative effects and that curcumin could be a genotoxic agent for some cell lines. We evaluated the genetic damage induced by curcumin to human lymphocytes exposed to increasing concentrations (0-50 lg/ml) of curcumin. Biomarkers such as chromosome aberrations (CAs) and sister chromatid exchange (SCE) were analyzed. In addition to the cytogenetic analysis, the effect of curcumin in the cell proliferation kinetics (CPK) by the proliferation index (PI) was also analyzed. The results indicated that high concentrations of curcumin induced CAs, mainly acentric fragments. SCEs rate was not statistically different from the control group in any curcumin treated cell group. The PI of cells treated with 2 and 5 lg/ml of curcumin were statistically significant from the control group and finally, the MI showed a tendency to increase in all the concentrations of curcumin tested. In conclusion, it can be assumed that the higher concentrations of curcumin evaluated have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.
Food and Chemical Toxicology, 2011
Enniatins (ENs) are N-methylated cyclohexadepsipeptides, secondary metabolites produced by variou... more Enniatins (ENs) are N-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus Fusarium. They are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. In this study we evaluated for the first time the antibiotic effect of pure fractions of EN J(1) and J(3) on several pathogenic strains and lactic acid bacteria. The ENs J(1) and J(3) were purified from the fermentation extract of Fusarium solani growth on solid medium of wheat kamut, using the technique of the low pressure liquid chromatography (LPLC) followed by a semipreparative liquid chromatography (LC). The purity and the structure of the isolated compound were confirmed by electrospray ionization-mass spectrometry study-linear ion trap (ESI-MS-LIT). The use of both chromatographic techniques have permitted to produce and purify 47mg of the En J(1) and 50mg of the EN J(3) with a mean purity of 98% completely characterized with the technique of the ESI-MS-LIT. Microbial bioassay analyses were carried out by incubation in MRSA and TSA for acid lactic and pathogenic bacteria, respectively during 24h at 37°C. None of the tested strains were inhibited by a 1ng dose of EN J(1) and J(3). These compounds were only not effective against Listeria monocytogenes, Pseudomonas aeruginosa and Salmonella enteric. This study highlight ENs J(1) and J(3) could be potentially effective antibacterial agents against several pathogenic and lactic acid bacteria.
According to the International Agency for Research on Cancer, smoking tobacco is a major cause of... more According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n = 48) and non-smokers (n = 53), aged from 18 to 49 years, was studied using SCE levels in peripheral lymphocytes. Heavy smoking (⩾10 cigarettes per day) increased significantly the SCE frequency and the HFC parameters. Neither age nor sex significantly influenced the frequencies in the groups studied.
Radiation Measurements, 2011
Many natural substances have been studied in recent past to be used as radioprotectors to mitigat... more Many natural substances have been studied in recent past to be used as radioprotectors to mitigate ionizing radiation-induced damage in mammalian systems due to its effectiveness given both pre- and post-irradiation and for long time with out drug-related toxicity. Curcumin and trans-resveratrol are both natural occurring polyphenols, obtained from the root of Curcuma longa and from grapes and other berries,
Nutrición hospitalaria, 2014
dietotherapeutical softwares are now a basic tool in the dietary management of patients, either f... more dietotherapeutical softwares are now a basic tool in the dietary management of patients, either from a physiological point of view and / or pathological. New technologies and research in this regard, have favored the emergence of new applications for the dietary and nutritional management that facilitate the management of the dietotherapeutical company. To comparatively study the main dietotherapeutical applications on the market to give criteria to the professional users of diet and nutrition in the selection of one of the main tools for these. Dietopro.com is, from our point of view, one of the most comprehensive management of patients dietotherapeutical applications. Based on the need of the user, it has different dietary sofwares choice.We conclude that there is no better or worse than another application, but applications roughly adapted to the needs of professionals.
Journal of the Science of Food and Agriculture, 2012
Resveratrol is a polyphenol with health properties being mainly present in the skins of several f... more Resveratrol is a polyphenol with health properties being mainly present in the skins of several foods. However, any study has been carried out to analyze the presence of this stilbene in the plum fruit from the genus Prunus in European and Japanese cultivars. The analysis of resveratrol from the skin in different cultivars of plums from Spanish markets with liquid chromatography coupled to ultraviolet (LC-UV) detector with subsequent confirmation by LC-MS/MS has been demonstrated that contents of this compound in plums ranged from 0.1 to 6.2 µg/g. Values of resveratrol in European plum cultivars is higher than in Japanese cultivars.
Journal of Agricultural and Food Chemistry, 2010
A method based on matrix solid phase dispersion extraction was applied to determine aflatoxins B(... more A method based on matrix solid phase dispersion extraction was applied to determine aflatoxins B(1), B(2), G(1), and G(2) from tigernuts and tigernut beverages. Recoveries of each aflatoxin from tigernut (spiked at 10 microg/kg level) and from tigernut beverages (spiked at 10 microg/L level) ranged from 72.3 to 82.1% and from 74.0 to 86.3%, respectively. The limits of quantification ranged from 0.21 to 1.49 microg/kg (for tigernuts) and from 0.13 to 0.57 microg/L (for tigernut beverages) studied using liquid chromatography with fluorescence detection. The proposed extraction method followed by liquid chromatography-fluorescence detection determination was applied to 37 and 25 samples of tigernuts and tigernut beverages, respectively, 3 positives being found in each category.
International Journal of Radiation Biology, 2013
Purpose : To survey the possible presence of chromosomal damage and internal contamination in a g... more Purpose : To survey the possible presence of chromosomal damage and internal contamination in a group of Ukrainian children and adolescents, 20 years after the Chernobyl accident at the Nuclear Power Plant. Materials and methods: Cytogenetical procedures were performed according to dicentric assay in 55 Ukrainian children and adolescents (29 boys and 26 girls), living near Chernobyl. In addition, a whole body detector and urinalysis were used to detect internal contamination. Results : 36 dicentrics were found in a total of 53,477 metaphases scored in these children, which refl ected a frequency of dicentrics below the background level. On the other hand, internal contamination was not detected in any subject studied. Conclusions : Since the estimated absorbed dose is below the detection limit, according to both biological and physical dosimetry, radiation overexposure during the last 3 -5 years has not been detected in the considered subjects.
Food Chemistry, 2011
A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ES... more A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB 1 , AFB 2 , AFG 1 and AFG 2 ) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C 18 , while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010. A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB 1 , AFB 2 and AFG 2 .
Food and Chemical Toxicology, 2014
According to the International Agency for Research on Cancer, smoking tobacco is a major cause of... more According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n = 48) and non-smokers (n = 53), aged from 18 to 49 years, was studied using SCE levels in peripheral lymphocytes. Heavy smoking (P10 cigarettes per day) increased significantly the SCE frequency and the HFC parameters. Neither age nor sex significantly influenced the frequencies in the groups studied.
Food and Chemical Toxicology, 2013
Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This... more Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This compound has shown antioxidant, anti-inflammatory, immunostimulant or anti-carcinogenic properties. Our aim was to evaluate the radioprotective efficacy, in vitro, of trans-resveratrol against radiationinduced chromosomal damage and to study the genotoxicity and cytotoxicity of this polyphenol in cell cultures without irradiation. The study was carried out by the pre-treatment of human lymphocytes at concentrations from 0 to 219 lM of trans-resveratrol. The results showed that all concentrations tested reduced radiation-induced chromosomal damage compared with cells with any treatment. Maximum damage protection was observed at the concentration of 2.19 lM. Concerning genotoxic results, all tested trans-resveratrol concentrations increased the sister chromatid exchange (SCE) index compared with no trans-resveratrol treatment. Cytotoxic indexes (Mitotic and Proliferation Index) showed that the lowest concentrations could enhance the cell proliferation rates and the highest ones could negatively affect to human peripheral lymphocytes growth.
Food and Chemical Toxicology, 2012
We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocyt... more We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000 lg ml À1 ). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.
Food and Chemical Toxicology, 2012
Curcumin has shown a wide range of properties such as anti-inflammatory and anti-carcinogenic pro... more Curcumin has shown a wide range of properties such as anti-inflammatory and anti-carcinogenic properties. Many of these effects, mainly the anti-carcinogenic effect, could be linked to its anti-oxidant effects. Nevertheless, some studies suggest that this natural compound possesses both pro-and antioxidative effects and that curcumin could be a genotoxic agent for some cell lines. We evaluated the genetic damage induced by curcumin to human lymphocytes exposed to increasing concentrations (0-50 lg/ml) of curcumin. Biomarkers such as chromosome aberrations (CAs) and sister chromatid exchange (SCE) were analyzed. In addition to the cytogenetic analysis, the effect of curcumin in the cell proliferation kinetics (CPK) by the proliferation index (PI) was also analyzed. The results indicated that high concentrations of curcumin induced CAs, mainly acentric fragments. SCEs rate was not statistically different from the control group in any curcumin treated cell group. The PI of cells treated with 2 and 5 lg/ml of curcumin were statistically significant from the control group and finally, the MI showed a tendency to increase in all the concentrations of curcumin tested. In conclusion, it can be assumed that the higher concentrations of curcumin evaluated have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.
Food and Chemical Toxicology, 2011
Enniatins (ENs) are N-methylated cyclohexadepsipeptides, secondary metabolites produced by variou... more Enniatins (ENs) are N-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus Fusarium. They are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. In this study we evaluated for the first time the antibiotic effect of pure fractions of EN J(1) and J(3) on several pathogenic strains and lactic acid bacteria. The ENs J(1) and J(3) were purified from the fermentation extract of Fusarium solani growth on solid medium of wheat kamut, using the technique of the low pressure liquid chromatography (LPLC) followed by a semipreparative liquid chromatography (LC). The purity and the structure of the isolated compound were confirmed by electrospray ionization-mass spectrometry study-linear ion trap (ESI-MS-LIT). The use of both chromatographic techniques have permitted to produce and purify 47mg of the En J(1) and 50mg of the EN J(3) with a mean purity of 98% completely characterized with the technique of the ESI-MS-LIT. Microbial bioassay analyses were carried out by incubation in MRSA and TSA for acid lactic and pathogenic bacteria, respectively during 24h at 37°C. None of the tested strains were inhibited by a 1ng dose of EN J(1) and J(3). These compounds were only not effective against Listeria monocytogenes, Pseudomonas aeruginosa and Salmonella enteric. This study highlight ENs J(1) and J(3) could be potentially effective antibacterial agents against several pathogenic and lactic acid bacteria.