Navnath Kashid - Academia.edu (original) (raw)
Papers by Navnath Kashid
Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is... more Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is valued immensely as a food, fodder for its role in biological nitrogen fixation and as an industrial raw material. Among the pulse crops, it stands first and has a great biological value. Looking at the immense applications of chickpea, the improvement in its yield potential and protein quality has become necessary. The present programme has been formulated for getting desirable plant types carrying beneficial alterations in plant habit, maturity characters, high yielding traits and increased level of seed proteins, in different mutant lines of that plant. For the present mutation breeding programme, BDN 9-3 and PG-5 cultivars of chickpea were used for induction of the mutations. By using different concentrations of two chemical mutagens EMS and SA mutations were induced in chickpea. The programme of mutation breeding was spread over three generations, viz. Mr, M, and Mr.The different t...
Asian Journal of Biochemistry, Genetics and Molecular Biology, 2021
This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphyloco... more This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, obtained from Microbiology laboratory, Al-Hikmah University Ilorin. Phytochemical analysis reveals the presence of tannins in all the three extracts (Aqueous, ethanolic and N-hexane), while. flavonoids, alkaloids and Saponins were only present in the Aqueous and ethanolic extracts. The leaves extracts were screened for antibacterial activity by agar well diffusion method, employing five different extracts concentrations (100mg/ml, 80mg/ml, 60mg/ml, 40mg/ml and 20mg/ml). The aqueous extracts had a mean activity of 11.50±0.70mm, 7.50±0.70mm and 8.5±0.70mm for S. aureus, E. coli and P. aeruginosa respectively. The ethanol extracts had a mean activity of 12.00±1.41mm, 10.00±1.41mm and 8.00±1.21mm for S. aureus, E. coli and P. aeruginosa respectively, while the N-hexane extracts exert no any activity. The antibiotics discs exert more i...
BIOINFOLET - A Quarterly Journal of Life Sciences, 2019
![Research paper thumbnail of Mutagenic Effect of Gamma Ray, Ethyl Methane Sulphonate and Sodium Azide on Seedling Height and Survival of Sunflower [Helianthus annus L.]](https://attachments.academia-assets.com/82905053/thumbnails/1.jpg)
](Seeds of sunflower [Helianthus annus L.] Bhanu& 55-56 were treated with various concentration of ... more Seeds of sunflower [Helianthus annus L.] Bhanu& 55-56 were treated with various concentration of Gammaray [10kR, 20kR, 30kR] Ems [0.05%, 0.10%, 0.15] & [0.01%,0.02%,0.03%] respectively, For studying seedling height [Shoot & root] and survival percentage of plants. In this investigation found that when the increasing concentration of mutagenes the decrease seedling height in compair to control. The data on plant survival in the present studies indicate that the survival percentage decreases with increase the mutagenic treatment
Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is... more Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is valued immensely as a food, fodder for its role in biological nitrogen fixation and as an industrial raw material. Among the pulse crops, it stands first and has a great biological value. Looking at the immense applications of chickpea, the improvement in its yield potential and protein quality has become necessary. The present programme has been formulated for getting desirable plant types carrying beneficial alterations in plant habit, maturity characters, high yielding traits and increased level of seed proteins, in different mutant lines of that plant. For the present mutation breeding programme, BDN 9-3 and PG-5 cultivars of chickpea were used for induction of the mutations. By using different concentrations of two chemical mutagens EMS and SA mutations were induced in chickpea. The programme of mutation breeding was spread over three generations, viz. Mr, M, and Mr.The different t...
Tropical Plant Research, 2016
In the present investigation of induced mutation in chickpea (Cicer arietinum L.) both the chemic... more In the present investigation of induced mutation in chickpea (Cicer arietinum L.) both the chemical mutagens (EMS and SA) succeeded in inducing variability in days to flowering of plants in both the cultivars. In case of SA treatments, an increasing trend with increasing concentrations was observable in both the cultivars of chickpea in M2 and M3 generations as regards days to flowering. The frequency of viable mutant showed the highest value at the 0.10% EMS and 0.02% SA concentration in both the cultivars of chickpea. Most of the viable mutants have been observed as true breeding in the subsequent M3 generation. They can be very well utilized on a commercial scale in view of the varied positive attributes carried by them.
BIOINFOLET - A Quarterly Journal of Life Sciences, 2019
It has been observed that the spectrum and frequency of viable mutations reduce with increasing c... more It has been observed that the spectrum and frequency of viable mutations reduce with increasing concentrations/doses of EMS, SAand gamma rays. This could be due to differential mode of action of the mutagens on different base sequences in various genes. A wide spectrum of viable mutants with varying morphological traits was observed in M2 generation of both the cultivars of Chickpea. The various types of viable mutants obtained in the two cultivars of Chickpea were Tall, Dwarf, Early maturing, Late maturing, High yielding, Anthoseed, Early flowering, Large pod, White seed and Small seed.
In the present investigation of Seeds Chickpea (Cicer arientinum L.) Were treated with different ... more In the present investigation of Seeds Chickpea (Cicer arientinum L.) Were treated with different doses of gamma radiation (10KR, 20KR, 30KR, ) concentration of sodium azide (0.02%, 0.04% &0.06%) and concentration of Ethyl methane sulphonate (0.05%, 0.10%&0.15%) For studying seed germination. The seed germination percentage was decreased with increased in the conc/doses when campared to control. The decrease in seed germination was more prominent with gamma rays than that at of EMS.
The present study aimed at evaluating the in vitro Phytochemical evaluation and antibacterial act... more The present study aimed at evaluating the in vitro Phytochemical evaluation and antibacterial activity of Aqueous, Chloroform, Ethanol, Methanol, Petrolium Ether extracts of Pongamia pinnata against Escherichia coli,(ATCC 25922),Salmonella Typhimurium,(734 MTCC).,Staphylococcus aureus (25923ATCC) Klebsella pneumonia (MTCC) and Pseudomonas aeruginosa(ATCC 27853), The various phytochemicals Alkaloid. Flavenod, Steroid, Phenol Glyceroids,Triglyceroids, Amino acid and Proteins are present in tested plant, which showed Antibacterial Activity against selected human pathogens..The Ethanolic extract showed the highest anti-S. typhiactivity and waseffective against all bacterial strains tested.Methanol, Chloroform, Aqueous extracts showed moderate activity and Petrolium Ether showed minimum inhibitory activity.
The present study aim is identification of maximum phytochemicals and its antibacterial effectaga... more The present study aim is identification of maximum phytochemicals and its antibacterial effectagainst some selected multidrug resistant human pathogens. The selected plant Dolichandronefalcata Seem. Showedpresence of alkaloids, phenolic, Flavonoids, terpenoids, tannins, steroidsbut absence of mucilage, Tryosine and Tryptophan in all solvent extracts. The plant extracts provedto be very potent against selected human pathogens, which are resistant to multidrugs. The resultsobtained by antibacterial activity showed equivalent antibacterial zone of inhibition to standardantibiotic Amoxicillin.
The induced mutations display a pleiotropic effect due to which several features are changed only... more The induced mutations display a pleiotropic effect due to which several features are changed only one of which however is desired by the breeder. The cross of two mutants can occasionally break undesired pleiotropic effect and valuable transgressions in other features can be achieved. Since majority of point mutations are recessive, the search for deviating phenotypes does not begin until the M 2 generation. The determination as to whether a detected variant is a mutant or non-heritable modification can only take place in M 2 generation after testing its progeny. Datta (1994) has indicated the utility of mutation technique in producing large number of new promising varieties in different ornamentals like chrysanthemums and roses. . The major objective in employing induced mutations has been to increase variability within shortest possible period and to develop some such genotypes which could carry attributes of immense economic value. Due to mutation breeding okra varieties increase...
n present investigation thin layer chromatography proved presence of different biomolecules. TheM... more n present investigation thin layer chromatography proved presence of different biomolecules. TheMethanol extract showed most potent activity among all, so it is tested by using GCMS analysis. Ten major compounds found in methanol extract. In our GCMS analysis observed that 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2), showed 18.68 % peak area which washighest in all identified ten compounds. The p-Cresol, 2-ehtyl (C9H12O) showed leastconcentration having peak area 1.45 and retention time was 17.3. The 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2) might responsible for effective antibacterial activity.Keywords: Dolichandrone falcata, TLC, GCMS analysis
The seeds of Okra varieties Parbhani Kranti and Arka Anamika were treated with physical and chemi... more The seeds of Okra varieties Parbhani Kranti and Arka Anamika were treated with physical and chemical mutagen to observe the pod length. In the present investigation different mutagens induced long pod mutants in both the varieties of okra viz. Parbhani Kranti and Arka Anamika. The mean values in regard to pod length demonstrated positive shift in mean values at 0.10% and 0.15% EMS treatments in Parbhani Kranti, while at remaining treatments the negative shift in mean values could be seen as compared with control. In Arka Anamika variety the mean values reduced at EMS and SA treatments and increased at gamma ray treatment.
![Research paper thumbnail of Effect of Physical and Chemical Mutagenesis in Sunflower [Helianthus Annus L.] on Seed Germination through Induced Mutation](https://attachments.academia-assets.com/77135124/thumbnails/1.jpg)
](International Journal of Science and Research (IJSR)
Sunflower [Helianthus annus L.] is an annual plant belongs to the compositae (Asteraceae) family.... more Sunflower [Helianthus annus L.] is an annual plant belongs to the compositae (Asteraceae) family. During present studies, the seed of sunflower varieties "Bhanu"; and "SS-56" were treated with different concentrations of chemical mutagens (0.05%, 0.10% & 0.15% for EMS and 0.01%, 0.02% & 0.03% for SA). The physical Mutagens like Gamma rays were used @ 10KR, 20R & 30 KR. After sowing in Petridis it was found that both varieties of sunflower responded differentially to EMS and Gamma rays treatments in respect of seed germination.
International Journal of Science and Research (IJSR)
Biochemical analysis of 11 different chickpea germplasm, viable mutants and about 200 micro mutan... more Biochemical analysis of 11 different chickpea germplasm, viable mutants and about 200 micro mutants was carried out for knowing the soluble seed protein content. The data regarding soluble seed protein content revealed substantial variability in all the germplasm lines and also different viable and micro mutants of both the cultivars of chickpea. Among 11 germplasm lines, the highest values for soluble seed protein content were recorded in chaffa and BG-256. The viable mutants in both the cultivars showed the variability in soluble seed protein content. In BDN 9-3, the highest value was observed in large leaf mutant while in PG-5, the late maturing mutant indicated highest value for soluble seed content. Thus, from the above observation, it can be said that mutagenic treatments tried in the present study have very much succeeded including genetic variability with significance alterations in growth and metabolism of the plant body. The result obtained decisively demonstrated the effective potential of the induced mutational approach in the genetic improvement of chickpea and recovering superior mutant types to helps obtain better desirable traits.
International Journal of Science and Research (IJSR)
In the present investigation the different quantitative characters in M2 and M3 generations were ... more In the present investigation the different quantitative characters in M2 and M3 generations were studied to estimate the variability induced by mutagenic treatments. A through statistical analysis of the data on individual quantitative character was done to analyses the effect of mutations in shifting the mean and variance in either direction. The effect of chemical mutagens on pod characters, the productivity in terms pods per plant as compared with control/ normal plants was found to be better in some of the viable mutants like tall, early maturing (in BDN 9-3) high yielding and late maturity. Elongated pod mutant was recorded only in BDN 9-3, in this mutant, the pods were found to be slightly elongated in shape as compared to control. The seeds of this mutant were larger, elongated and more weighted than the control.
Research Journal of Pharmacognosy and Phytochemistry
Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is... more Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is valued immensely as a food, fodder for its role in biological nitrogen fixation and as an industrial raw material. Among the pulse crops, it stands first and has a great biological value. Looking at the immense applications of chickpea, the improvement in its yield potential and protein quality has become necessary. The present programme has been formulated for getting desirable plant types carrying beneficial alterations in plant habit, maturity characters, high yielding traits and increased level of seed proteins, in different mutant lines of that plant. For the present mutation breeding programme, BDN 9-3 and PG-5 cultivars of chickpea were used for induction of the mutations. By using different concentrations of two chemical mutagens EMS and SA mutations were induced in chickpea. The programme of mutation breeding was spread over three generations, viz. Mr, M, and Mr.The different t...
Asian Journal of Biochemistry, Genetics and Molecular Biology, 2021
This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphyloco... more This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, obtained from Microbiology laboratory, Al-Hikmah University Ilorin. Phytochemical analysis reveals the presence of tannins in all the three extracts (Aqueous, ethanolic and N-hexane), while. flavonoids, alkaloids and Saponins were only present in the Aqueous and ethanolic extracts. The leaves extracts were screened for antibacterial activity by agar well diffusion method, employing five different extracts concentrations (100mg/ml, 80mg/ml, 60mg/ml, 40mg/ml and 20mg/ml). The aqueous extracts had a mean activity of 11.50±0.70mm, 7.50±0.70mm and 8.5±0.70mm for S. aureus, E. coli and P. aeruginosa respectively. The ethanol extracts had a mean activity of 12.00±1.41mm, 10.00±1.41mm and 8.00±1.21mm for S. aureus, E. coli and P. aeruginosa respectively, while the N-hexane extracts exert no any activity. The antibiotics discs exert more i...
BIOINFOLET - A Quarterly Journal of Life Sciences, 2019
Seeds of sunflower [Helianthus annus L.] Bhanu& 55-56 were treated with various concentration of ... more Seeds of sunflower [Helianthus annus L.] Bhanu& 55-56 were treated with various concentration of Gammaray [10kR, 20kR, 30kR] Ems [0.05%, 0.10%, 0.15] & [0.01%,0.02%,0.03%] respectively, For studying seedling height [Shoot & root] and survival percentage of plants. In this investigation found that when the increasing concentration of mutagenes the decrease seedling height in compair to control. The data on plant survival in the present studies indicate that the survival percentage decreases with increase the mutagenic treatment
Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is... more Chickpea, (Cicer orietinum L.) is an important grain legume plant having multifarious uses. lt is valued immensely as a food, fodder for its role in biological nitrogen fixation and as an industrial raw material. Among the pulse crops, it stands first and has a great biological value. Looking at the immense applications of chickpea, the improvement in its yield potential and protein quality has become necessary. The present programme has been formulated for getting desirable plant types carrying beneficial alterations in plant habit, maturity characters, high yielding traits and increased level of seed proteins, in different mutant lines of that plant. For the present mutation breeding programme, BDN 9-3 and PG-5 cultivars of chickpea were used for induction of the mutations. By using different concentrations of two chemical mutagens EMS and SA mutations were induced in chickpea. The programme of mutation breeding was spread over three generations, viz. Mr, M, and Mr.The different t...
Tropical Plant Research, 2016
In the present investigation of induced mutation in chickpea (Cicer arietinum L.) both the chemic... more In the present investigation of induced mutation in chickpea (Cicer arietinum L.) both the chemical mutagens (EMS and SA) succeeded in inducing variability in days to flowering of plants in both the cultivars. In case of SA treatments, an increasing trend with increasing concentrations was observable in both the cultivars of chickpea in M2 and M3 generations as regards days to flowering. The frequency of viable mutant showed the highest value at the 0.10% EMS and 0.02% SA concentration in both the cultivars of chickpea. Most of the viable mutants have been observed as true breeding in the subsequent M3 generation. They can be very well utilized on a commercial scale in view of the varied positive attributes carried by them.
BIOINFOLET - A Quarterly Journal of Life Sciences, 2019
It has been observed that the spectrum and frequency of viable mutations reduce with increasing c... more It has been observed that the spectrum and frequency of viable mutations reduce with increasing concentrations/doses of EMS, SAand gamma rays. This could be due to differential mode of action of the mutagens on different base sequences in various genes. A wide spectrum of viable mutants with varying morphological traits was observed in M2 generation of both the cultivars of Chickpea. The various types of viable mutants obtained in the two cultivars of Chickpea were Tall, Dwarf, Early maturing, Late maturing, High yielding, Anthoseed, Early flowering, Large pod, White seed and Small seed.
In the present investigation of Seeds Chickpea (Cicer arientinum L.) Were treated with different ... more In the present investigation of Seeds Chickpea (Cicer arientinum L.) Were treated with different doses of gamma radiation (10KR, 20KR, 30KR, ) concentration of sodium azide (0.02%, 0.04% &0.06%) and concentration of Ethyl methane sulphonate (0.05%, 0.10%&0.15%) For studying seed germination. The seed germination percentage was decreased with increased in the conc/doses when campared to control. The decrease in seed germination was more prominent with gamma rays than that at of EMS.
The present study aimed at evaluating the in vitro Phytochemical evaluation and antibacterial act... more The present study aimed at evaluating the in vitro Phytochemical evaluation and antibacterial activity of Aqueous, Chloroform, Ethanol, Methanol, Petrolium Ether extracts of Pongamia pinnata against Escherichia coli,(ATCC 25922),Salmonella Typhimurium,(734 MTCC).,Staphylococcus aureus (25923ATCC) Klebsella pneumonia (MTCC) and Pseudomonas aeruginosa(ATCC 27853), The various phytochemicals Alkaloid. Flavenod, Steroid, Phenol Glyceroids,Triglyceroids, Amino acid and Proteins are present in tested plant, which showed Antibacterial Activity against selected human pathogens..The Ethanolic extract showed the highest anti-S. typhiactivity and waseffective against all bacterial strains tested.Methanol, Chloroform, Aqueous extracts showed moderate activity and Petrolium Ether showed minimum inhibitory activity.
The present study aim is identification of maximum phytochemicals and its antibacterial effectaga... more The present study aim is identification of maximum phytochemicals and its antibacterial effectagainst some selected multidrug resistant human pathogens. The selected plant Dolichandronefalcata Seem. Showedpresence of alkaloids, phenolic, Flavonoids, terpenoids, tannins, steroidsbut absence of mucilage, Tryosine and Tryptophan in all solvent extracts. The plant extracts provedto be very potent against selected human pathogens, which are resistant to multidrugs. The resultsobtained by antibacterial activity showed equivalent antibacterial zone of inhibition to standardantibiotic Amoxicillin.
The induced mutations display a pleiotropic effect due to which several features are changed only... more The induced mutations display a pleiotropic effect due to which several features are changed only one of which however is desired by the breeder. The cross of two mutants can occasionally break undesired pleiotropic effect and valuable transgressions in other features can be achieved. Since majority of point mutations are recessive, the search for deviating phenotypes does not begin until the M 2 generation. The determination as to whether a detected variant is a mutant or non-heritable modification can only take place in M 2 generation after testing its progeny. Datta (1994) has indicated the utility of mutation technique in producing large number of new promising varieties in different ornamentals like chrysanthemums and roses. . The major objective in employing induced mutations has been to increase variability within shortest possible period and to develop some such genotypes which could carry attributes of immense economic value. Due to mutation breeding okra varieties increase...
n present investigation thin layer chromatography proved presence of different biomolecules. TheM... more n present investigation thin layer chromatography proved presence of different biomolecules. TheMethanol extract showed most potent activity among all, so it is tested by using GCMS analysis. Ten major compounds found in methanol extract. In our GCMS analysis observed that 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2), showed 18.68 % peak area which washighest in all identified ten compounds. The p-Cresol, 2-ehtyl (C9H12O) showed leastconcentration having peak area 1.45 and retention time was 17.3. The 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2) might responsible for effective antibacterial activity.Keywords: Dolichandrone falcata, TLC, GCMS analysis
The seeds of Okra varieties Parbhani Kranti and Arka Anamika were treated with physical and chemi... more The seeds of Okra varieties Parbhani Kranti and Arka Anamika were treated with physical and chemical mutagen to observe the pod length. In the present investigation different mutagens induced long pod mutants in both the varieties of okra viz. Parbhani Kranti and Arka Anamika. The mean values in regard to pod length demonstrated positive shift in mean values at 0.10% and 0.15% EMS treatments in Parbhani Kranti, while at remaining treatments the negative shift in mean values could be seen as compared with control. In Arka Anamika variety the mean values reduced at EMS and SA treatments and increased at gamma ray treatment.
International Journal of Science and Research (IJSR)
Sunflower [Helianthus annus L.] is an annual plant belongs to the compositae (Asteraceae) family.... more Sunflower [Helianthus annus L.] is an annual plant belongs to the compositae (Asteraceae) family. During present studies, the seed of sunflower varieties "Bhanu"; and "SS-56" were treated with different concentrations of chemical mutagens (0.05%, 0.10% & 0.15% for EMS and 0.01%, 0.02% & 0.03% for SA). The physical Mutagens like Gamma rays were used @ 10KR, 20R & 30 KR. After sowing in Petridis it was found that both varieties of sunflower responded differentially to EMS and Gamma rays treatments in respect of seed germination.
International Journal of Science and Research (IJSR)
Biochemical analysis of 11 different chickpea germplasm, viable mutants and about 200 micro mutan... more Biochemical analysis of 11 different chickpea germplasm, viable mutants and about 200 micro mutants was carried out for knowing the soluble seed protein content. The data regarding soluble seed protein content revealed substantial variability in all the germplasm lines and also different viable and micro mutants of both the cultivars of chickpea. Among 11 germplasm lines, the highest values for soluble seed protein content were recorded in chaffa and BG-256. The viable mutants in both the cultivars showed the variability in soluble seed protein content. In BDN 9-3, the highest value was observed in large leaf mutant while in PG-5, the late maturing mutant indicated highest value for soluble seed content. Thus, from the above observation, it can be said that mutagenic treatments tried in the present study have very much succeeded including genetic variability with significance alterations in growth and metabolism of the plant body. The result obtained decisively demonstrated the effective potential of the induced mutational approach in the genetic improvement of chickpea and recovering superior mutant types to helps obtain better desirable traits.
International Journal of Science and Research (IJSR)
In the present investigation the different quantitative characters in M2 and M3 generations were ... more In the present investigation the different quantitative characters in M2 and M3 generations were studied to estimate the variability induced by mutagenic treatments. A through statistical analysis of the data on individual quantitative character was done to analyses the effect of mutations in shifting the mean and variance in either direction. The effect of chemical mutagens on pod characters, the productivity in terms pods per plant as compared with control/ normal plants was found to be better in some of the viable mutants like tall, early maturing (in BDN 9-3) high yielding and late maturity. Elongated pod mutant was recorded only in BDN 9-3, in this mutant, the pods were found to be slightly elongated in shape as compared to control. The seeds of this mutant were larger, elongated and more weighted than the control.
Research Journal of Pharmacognosy and Phytochemistry