Nazaré Bissoli - Academia.edu (original) (raw)

Papers by Nazaré Bissoli

Nutrition, Metabolism and Cardiovascular Diseases, 2019

NS, Chronic metformin reduces systemic and local inflammatory proteins and improves hypertension-... more NS, Chronic metformin reduces systemic and local inflammatory proteins and improves hypertension-related cardiac autonomic dysfunction, Nutrition, Metabolism and Cardiovascular Diseases,

Life Sciences, 2018

Nandrolone alter left ventricular contractility and promotes remodelling involving calcium-handli... more Nandrolone alter left ventricular contractility and promotes remodelling involving calcium-handling proteins and renin-angiotensin system in male SHR. Lfs (2018),

Life Sciences, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

The FASEB Journal, 2015

Introduction: In the present study, we compared telmisartan, an angiotensin AT1 receptor blocker ... more Introduction: In the present study, we compared telmisartan, an angiotensin AT1 receptor blocker (ARB), and treadmill running exercise training on bone mineral density (BMD) and biomechanical prope...

Anais Brasileiros de Dermatologia, 2005

FUNDAMENTOS: A difusão do ultra-som (US) como método terapêutico corroborou o perfil de segurança... more FUNDAMENTOS: A difusão do ultra-som (US) como método terapêutico corroborou o perfil de segurança observado na prática médica, porém nem sempre baseado em estudos sistemáticos, sugerindo medidas de cautela e otimização da técnica. OBJETIVO: Avaliar o risco potencial da utilização do ultra-som de baixa intensidade em terapias dermatológicas e estéticas e suas implicações no sistema cardiovascular. MÉTODOS: Utilizaram-se (n=10) ratas Wistar, pesando +300g e divididas em dois grupos: controle-Sham e terapia ultra-sônica (TUS). Após anestesia induzida por halotano, as fêmeas foram submetidas à TUS modo de pulso, com potência de 1W/cm² e 3MHz freqüência, na região inguinal em área de 3cm², durante três minutos por 10 dias. Ao final do tratamento, após anestesia com (cloral hidratado 10% / 0,4ml/100g), foram cateterizadas em artéria femoral, e mensuradas a pressão arterial média (PAM) e a freqüência cardíaca (FC); foi coletado sangue para dosagem do perfil lipídico e glicêmico. A seguir, ...

Research, Society and Development

Background: Juçara açaí is a Brazilian berry with high anthocyanidin content and high antioxidant... more Background: Juçara açaí is a Brazilian berry with high anthocyanidin content and high antioxidant properties. A correlation between oxidative stress, hypertension and baroreflex impairment is known, however, no previous studies investigate the effect of juçara fruit pulp on hypertension. Aim: The aim of this study was to investigate antihypertensive effect of juçara (Euterpe edulis) fruit pulp in SHR animals, its antioxidant capacity and influence on baroreflex sensitivity. Methods: Chemical profile was obtained by ESI(-)-FT-ICR-MS. For biological analysis, male SHR were separated into groups: control (S), which received water; captopril (SC), which received captopril (30 mg/kg), and juçara (SJ), which received juçara pulp (4.4mL/kg). Treatments were daily performed by gavage and remained for 8 weeks. Baroreflex evaluation was made by in bolus injection of phenylephrine and sodium nitroprusside, after the analysis the heart was frozen (-80ºC) for biomolecular evaluations (AOPP, TBAR...

Research, Society and Development, 2022

Stanozolol is a steroid that causes lipid deposition in LDLr-/- mice, although the mechanism by w... more Stanozolol is a steroid that causes lipid deposition in LDLr-/- mice, although the mechanism by which this dyslipidemia results in cardiac dysfunction is little understood. The aim of this study was to evaluate the effect of stanozolol on cardiac contractility and the participation of myocardial phospholamban (pPLB) phosphorylation in an atherosclerosis mouse model. LDL receptor knockout mice (LDLr-/-) were fed a standard chow diet and received weekly subcutaneous injections of either saline (control, C group) or 20 mg/kg stanozolol (S group). After 8 weeks, hemodynamic parameters were assessed in the left ventricle. The heart was collected, weighted for hypertrophy evaluation, and kept in formalin buffer for morphometric analysis (H&E) and collagen quantification (Picrossirius). Protein expression of PLB and its phosphorylated form (p-PLB) in the left ventricle was determined by western blot. We observed that stanozolol treatment favored cardiac hypertrophy and collagen deposition ...

The FASEB Journal, 2015

There are several evidences of cardiovascular damage by use anabolic steroids androgenic (AAS). H... more There are several evidences of cardiovascular damage by use anabolic steroids androgenic (AAS). However, little is known about the cardiac effects of abusive use of AAS by women. Aim of this study ...

Toxicology and applied pharmacology, Jun 10, 2016

Anabolic-androgenic steroids are misused, including by women, but little is known about the cardi... more Anabolic-androgenic steroids are misused, including by women, but little is known about the cardiovascular effects of these drugs on women. To evaluated the effects of nandrolone decanoate (ND) and resistive physical exercise on cardiac contractility in young female rats. Female Wistar rats were separated into 4 groups: C (untrained animals); E (animals were submitted to resistance exercise by jumping in water 5 times per week); ND (animals were treated with ND, 20mg/kg/week for 4weeks); and NDE (trained and treated). The haemodynamic parameters (+dP/dtmax, -dP/dtmin and Tau) were assessed in the left ventricle. The heart was collected for histological analyses and collagen deposition. The gastrocnemius muscle was weighed, and hypertrophy was assessed by the ratio of their weights to gastrocnemius/tibia length. The expression of calcium handling proteins was measured by western blot analysis. ND treatment and physical exercise increased cardiac contractility and relaxation. In addit...

Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1995

Heart tissue contains large amounts of the Ca(2+)-activated proteinase calpain which has been ass... more Heart tissue contains large amounts of the Ca(2+)-activated proteinase calpain which has been assigned a specific function in the turnover of muscle protein. The objective of the present study was to determine calpain (E.C. 3.4.22.17)-like activity in homogenates of left ventricle from hypertensive rats that developed ventricular hypertrophy. Calpain activity was assayed using heat-denatured azocasein as a substrate in the presence of 1 mM calcium and corrected by subtraction of the Ca(2+)-independent activities. The latter were measured in the presence of 1 mM EGTA and the products read at 440 nm. Male Wistar rats (225 g) were assigned to control (N = 8, normal drinking water), salt (N = 6, drinking water containing 1% NaCl) and DOCA-salt (N = 6, deoxycorticosterone acetate, 8 mg/kg, sc, twice a week for 20 days plus drinking water containing 1% NaCl) groups. SHR (N = 6, spontaneously hypertensive rats) were also used. The calpain activity of the control group was at 3.90 +/- 0.22 ...

Sleep and Breathing, 2013

The aim of this study was to investigate the efficacy of continuous positive airway pressure (CPA... more The aim of this study was to investigate the efficacy of continuous positive airway pressure (CPAP) therapy following uvulopalatopharyngoplasty (UPPP) to prevent blood pressure (BP) elevation during sleep. Sixteen normotensive patients with OSA were subjected to UPPP with or without septoplasty. These patients were instrumented for 24 h of ambulatory BP recording, polysomnography, nocturnal urinary catecholamine and pain evaluation using a visual analogue scale in the day prior to surgery (D-1), following the surgery (D+1) and 30 days later (D+30). For the D+1, the patients were divided into two groups: the without CPAP therapy group and the with CPAP therapy group. The apnoea-hypopnoea index (AHI) significantly increased in the patients without CPAP therapy compared with the D-1 (74 ± 23 vs. 35 ± 6 times/h, p < 0.05), and in the CPAP group, there was a significant reduction in the average AHI value to 14 ± 6 times/h, p < 0.01. During D+1, we observed an increase in the nocturnal systolic BP (10 %), diastolic BP (12 %) and heart rate (14 %) in the group without CPAP. These metrics were re-established in the CPAP group to values that were similar to those that were observed on the D-1. The absence of nocturnal dipping in the group without CPAP was followed by a significant increase in nocturnal norepinephrine (42 ± 12 μg/l/12 h) and epinephrine (8 ± 2 μg/l/12 h) levels compared with the D-1 (norepinephrine 17 ± 3; epinephrine 2 ± 0.3 μg/l/12 h, p < 0.001). In the patients who used the CPAP treatment, the nocturnal catecholamine levels were similar to D-1. The…

Life sciences, Jan 23, 2015

Endothelial dysfunction is considered a premature indication of atherosclerosis and vessel damage... more Endothelial dysfunction is considered a premature indication of atherosclerosis and vessel damage and is present in the postmenopausal period. This study compares the influence of estrogen, raloxifene and tamoxifen on factors that affect endothelial function in ovariectomized (OVX) rats. The rats were divided into: SHAM; OVX; OVX+Estrogen (0.5μg/Kg/day); OVX+Raloxifene (2mg/Kg/day) and OVX+Tamoxifen (1mg/Kg/day) groups. The acetylcholine vasorelaxation response was evaluated in the mesenteric vascular bed. The vascular oxidative stress and serum inflammatory cytokines levels were monitored, and analyses of eNOS and iNOS were performed. The acetylcholine-induced responses obtained in the OVX were lower than those obtained in the SHAM, and all treatments restored this response. L-NAME reduced and equalized the acetylcholine-induced response in all groups. The attenuation of the acetylcholine-induced responses by aminoguanidine was greater in the OVX. Endothelial dysfunction in OVX was...

Pharmacological Research, 2000

Pharmacological Research, 1996

This study was carried out to test the effect of chlorthalidone (Chlor) treatment on the vascular... more This study was carried out to test the effect of chlorthalidone (Chlor) treatment on the vascular responsiveness to acetylcholine (Ach) and adenosine disphosphate (ADP) under different extracellular calcium concentrations (Cae2+) in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Half of the DOCA-salt hypertensive rats were simultaneously given Chlor (8 mg day-1, p.o.), after the twentieth day of DOCA, until the fortieth day. Mean arterial pressure (MAP) was reduced (P < 0.01) in DOCA-salt-Chlor (121 +/- 3 mmHg) compared to DOCA-salt (175 +/- 3 mmHg) rats. Chlor reduced the high plasma sodium of DOCA-salt to levels of the normotensive groups. The basal perfusion pressure of the isolated mesenteric vascular bed (MVB) was not modified by Cae2+ changing or previous addition of ouabain. At normal and low Cae2+ the vasodilator effect of Ach in the MVB was reduced (P < 0.01) in DOCA-salt and less (P < 0.05) in the salt-control rats. Chlor shifted the curve of DOCA-salt backward to control group curves. In high Cae2+ the vasodilator responses to Ach were reduced (P < 0.01) both in normal-control and DOCA-salt+Chlor but not in salt-control but not in salt-control and DOCA-salt group. The vasodilator effect of ADP to different Cae2+ was similar in presence of normal and low Cae2+ in normal-control rats and it was reduced (P < 0.01) in presence of high Cae2+ in the DOCA-salt group. Chlor restored the responses to ADP to normal-control level. Under ouabain-treatment, the vasodilation duration was reduced (P < 0.05) in all groups. It was shorter (P < 0.01) in DOCA-salt than in the other ones. Chlor completely abolished this difference. The MAP reduction in DOCA-salt rats by chlor could also be associated with a restored normal responsiveness of endothelial cells to endogenous vasodilator, for example Ach and ADP, since sodium nitroprusside did not exhibit differences among the various treatments. This diuretic agent seems to elicit its effect through a reduction in plasma sodium content of DOCA-salt hypertensive rats.

Hypertension, 1994

The present study examined the time course of changes in baroreceptor reflex function by means of... more The present study examined the time course of changes in baroreceptor reflex function by means of sigmoidal curve-fitting analysis in conscious, unrestrained renovascular one-kidney, one clip (1K1C) rats at 1, 3, 7, 15, 30, and 60 days after renal artery clipping. The reflex heart rate responses were elicited by alternate intravenous bolus injections of phenylephrine (change, +5 to +50 mm Hg) and sodium nitroprusside (change, -5 to -50 mmHg). Atropine methylnitrate and atenolol were given to evaluate the responses mediated by the cardiac sympathetic or vagal component, respectively. The average baroreceptor reflex gain (sensitivity) decreased progressively (day 1, 3.35 +/- 0.3 beats per minute [bpm] per millimeter of mercury), reaching a maximal attenuation in the 30-day 1K1C group (1.83 +/- 0.5 bpm/mm Hg) compared with sham rats (approximately 4.60 bpm/mm Hg). The data showed a decreased vagal activity contributing to the attenuation of the baroreceptor gain only in the 30-day 1K1C...

Dermatologic Surgery, 2009

Clinical and Experimental Hypertension, 2010

The role of renal nerve in excretion was investigated during acute obstructive apnea (OA) episode... more The role of renal nerve in excretion was investigated during acute obstructive apnea (OA) episodes in SHR. The animals (SHR and control, C) were presented for renal denervation (D; CD; SHRD) or undenervation (U; CU; SHRU). Tracheal catheterization was performed to induce OA via its total occlusion. Urine samples were collected every 2 min after 20 s of OA. Obstructive apnea resulted in bradycardia, hypotension, and induced elevations in the urinary measurements in SHRU, but not in CU. Conversely, the denervation increased in CD, but not in the SHRD. Urinary excretion was dependent of renal nerve in SHR during OA.

Archives of Medical Research, 2008

Journal of International Medical Research, 1993

Chronic ethanol administration causes hypertension and alterations of vascular reactivity in rats... more Chronic ethanol administration causes hypertension and alterations of vascular reactivity in rats. In several models of hypertension, alterations of vascular reactivity are believed to be secondary to the sustained increase in blood pressure. The present study investigated the effects of serotonin (5-hydroxytryptamine [5-HT]), vasopressin and acetylcholine (ACh) in the isolated perfused mesenteric arteries from Wistar rats submitted to an 8-week course of chronic ethanol intake (8 g/kg day). No significant differences were observed in the dose – response curves with regard to: pressor effect of 0.04 — 10.0 nmole 5-HT; relaxant effect of 0.05 — 50.0 nmole ACh; or the pressor effects of two 1.5-nmole doses of vasopressin between control rats and ethanol-fed rats. These results suggest that modifications in arterial reactivity to endogenous vasoactive substances (observed in other studies involving more prolonged ethanol treatment in rats) may be, in part, secondary to the increase in ...

Nutrition, Metabolism and Cardiovascular Diseases, 2019

NS, Chronic metformin reduces systemic and local inflammatory proteins and improves hypertension-... more NS, Chronic metformin reduces systemic and local inflammatory proteins and improves hypertension-related cardiac autonomic dysfunction, Nutrition, Metabolism and Cardiovascular Diseases,

Life Sciences, 2018

Nandrolone alter left ventricular contractility and promotes remodelling involving calcium-handli... more Nandrolone alter left ventricular contractility and promotes remodelling involving calcium-handling proteins and renin-angiotensin system in male SHR. Lfs (2018),

Life Sciences, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

The FASEB Journal, 2015

Introduction: In the present study, we compared telmisartan, an angiotensin AT1 receptor blocker ... more Introduction: In the present study, we compared telmisartan, an angiotensin AT1 receptor blocker (ARB), and treadmill running exercise training on bone mineral density (BMD) and biomechanical prope...

Anais Brasileiros de Dermatologia, 2005

FUNDAMENTOS: A difusão do ultra-som (US) como método terapêutico corroborou o perfil de segurança... more FUNDAMENTOS: A difusão do ultra-som (US) como método terapêutico corroborou o perfil de segurança observado na prática médica, porém nem sempre baseado em estudos sistemáticos, sugerindo medidas de cautela e otimização da técnica. OBJETIVO: Avaliar o risco potencial da utilização do ultra-som de baixa intensidade em terapias dermatológicas e estéticas e suas implicações no sistema cardiovascular. MÉTODOS: Utilizaram-se (n=10) ratas Wistar, pesando +300g e divididas em dois grupos: controle-Sham e terapia ultra-sônica (TUS). Após anestesia induzida por halotano, as fêmeas foram submetidas à TUS modo de pulso, com potência de 1W/cm² e 3MHz freqüência, na região inguinal em área de 3cm², durante três minutos por 10 dias. Ao final do tratamento, após anestesia com (cloral hidratado 10% / 0,4ml/100g), foram cateterizadas em artéria femoral, e mensuradas a pressão arterial média (PAM) e a freqüência cardíaca (FC); foi coletado sangue para dosagem do perfil lipídico e glicêmico. A seguir, ...

Research, Society and Development

Background: Juçara açaí is a Brazilian berry with high anthocyanidin content and high antioxidant... more Background: Juçara açaí is a Brazilian berry with high anthocyanidin content and high antioxidant properties. A correlation between oxidative stress, hypertension and baroreflex impairment is known, however, no previous studies investigate the effect of juçara fruit pulp on hypertension. Aim: The aim of this study was to investigate antihypertensive effect of juçara (Euterpe edulis) fruit pulp in SHR animals, its antioxidant capacity and influence on baroreflex sensitivity. Methods: Chemical profile was obtained by ESI(-)-FT-ICR-MS. For biological analysis, male SHR were separated into groups: control (S), which received water; captopril (SC), which received captopril (30 mg/kg), and juçara (SJ), which received juçara pulp (4.4mL/kg). Treatments were daily performed by gavage and remained for 8 weeks. Baroreflex evaluation was made by in bolus injection of phenylephrine and sodium nitroprusside, after the analysis the heart was frozen (-80ºC) for biomolecular evaluations (AOPP, TBAR...

Research, Society and Development, 2022

Stanozolol is a steroid that causes lipid deposition in LDLr-/- mice, although the mechanism by w... more Stanozolol is a steroid that causes lipid deposition in LDLr-/- mice, although the mechanism by which this dyslipidemia results in cardiac dysfunction is little understood. The aim of this study was to evaluate the effect of stanozolol on cardiac contractility and the participation of myocardial phospholamban (pPLB) phosphorylation in an atherosclerosis mouse model. LDL receptor knockout mice (LDLr-/-) were fed a standard chow diet and received weekly subcutaneous injections of either saline (control, C group) or 20 mg/kg stanozolol (S group). After 8 weeks, hemodynamic parameters were assessed in the left ventricle. The heart was collected, weighted for hypertrophy evaluation, and kept in formalin buffer for morphometric analysis (H&E) and collagen quantification (Picrossirius). Protein expression of PLB and its phosphorylated form (p-PLB) in the left ventricle was determined by western blot. We observed that stanozolol treatment favored cardiac hypertrophy and collagen deposition ...

The FASEB Journal, 2015

There are several evidences of cardiovascular damage by use anabolic steroids androgenic (AAS). H... more There are several evidences of cardiovascular damage by use anabolic steroids androgenic (AAS). However, little is known about the cardiac effects of abusive use of AAS by women. Aim of this study ...

Toxicology and applied pharmacology, Jun 10, 2016

Anabolic-androgenic steroids are misused, including by women, but little is known about the cardi... more Anabolic-androgenic steroids are misused, including by women, but little is known about the cardiovascular effects of these drugs on women. To evaluated the effects of nandrolone decanoate (ND) and resistive physical exercise on cardiac contractility in young female rats. Female Wistar rats were separated into 4 groups: C (untrained animals); E (animals were submitted to resistance exercise by jumping in water 5 times per week); ND (animals were treated with ND, 20mg/kg/week for 4weeks); and NDE (trained and treated). The haemodynamic parameters (+dP/dtmax, -dP/dtmin and Tau) were assessed in the left ventricle. The heart was collected for histological analyses and collagen deposition. The gastrocnemius muscle was weighed, and hypertrophy was assessed by the ratio of their weights to gastrocnemius/tibia length. The expression of calcium handling proteins was measured by western blot analysis. ND treatment and physical exercise increased cardiac contractility and relaxation. In addit...

Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1995

Heart tissue contains large amounts of the Ca(2+)-activated proteinase calpain which has been ass... more Heart tissue contains large amounts of the Ca(2+)-activated proteinase calpain which has been assigned a specific function in the turnover of muscle protein. The objective of the present study was to determine calpain (E.C. 3.4.22.17)-like activity in homogenates of left ventricle from hypertensive rats that developed ventricular hypertrophy. Calpain activity was assayed using heat-denatured azocasein as a substrate in the presence of 1 mM calcium and corrected by subtraction of the Ca(2+)-independent activities. The latter were measured in the presence of 1 mM EGTA and the products read at 440 nm. Male Wistar rats (225 g) were assigned to control (N = 8, normal drinking water), salt (N = 6, drinking water containing 1% NaCl) and DOCA-salt (N = 6, deoxycorticosterone acetate, 8 mg/kg, sc, twice a week for 20 days plus drinking water containing 1% NaCl) groups. SHR (N = 6, spontaneously hypertensive rats) were also used. The calpain activity of the control group was at 3.90 +/- 0.22 ...

Sleep and Breathing, 2013

The aim of this study was to investigate the efficacy of continuous positive airway pressure (CPA... more The aim of this study was to investigate the efficacy of continuous positive airway pressure (CPAP) therapy following uvulopalatopharyngoplasty (UPPP) to prevent blood pressure (BP) elevation during sleep. Sixteen normotensive patients with OSA were subjected to UPPP with or without septoplasty. These patients were instrumented for 24 h of ambulatory BP recording, polysomnography, nocturnal urinary catecholamine and pain evaluation using a visual analogue scale in the day prior to surgery (D-1), following the surgery (D+1) and 30 days later (D+30). For the D+1, the patients were divided into two groups: the without CPAP therapy group and the with CPAP therapy group. The apnoea-hypopnoea index (AHI) significantly increased in the patients without CPAP therapy compared with the D-1 (74 ± 23 vs. 35 ± 6 times/h, p < 0.05), and in the CPAP group, there was a significant reduction in the average AHI value to 14 ± 6 times/h, p < 0.01. During D+1, we observed an increase in the nocturnal systolic BP (10 %), diastolic BP (12 %) and heart rate (14 %) in the group without CPAP. These metrics were re-established in the CPAP group to values that were similar to those that were observed on the D-1. The absence of nocturnal dipping in the group without CPAP was followed by a significant increase in nocturnal norepinephrine (42 ± 12 μg/l/12 h) and epinephrine (8 ± 2 μg/l/12 h) levels compared with the D-1 (norepinephrine 17 ± 3; epinephrine 2 ± 0.3 μg/l/12 h, p < 0.001). In the patients who used the CPAP treatment, the nocturnal catecholamine levels were similar to D-1. The…

Life sciences, Jan 23, 2015

Endothelial dysfunction is considered a premature indication of atherosclerosis and vessel damage... more Endothelial dysfunction is considered a premature indication of atherosclerosis and vessel damage and is present in the postmenopausal period. This study compares the influence of estrogen, raloxifene and tamoxifen on factors that affect endothelial function in ovariectomized (OVX) rats. The rats were divided into: SHAM; OVX; OVX+Estrogen (0.5μg/Kg/day); OVX+Raloxifene (2mg/Kg/day) and OVX+Tamoxifen (1mg/Kg/day) groups. The acetylcholine vasorelaxation response was evaluated in the mesenteric vascular bed. The vascular oxidative stress and serum inflammatory cytokines levels were monitored, and analyses of eNOS and iNOS were performed. The acetylcholine-induced responses obtained in the OVX were lower than those obtained in the SHAM, and all treatments restored this response. L-NAME reduced and equalized the acetylcholine-induced response in all groups. The attenuation of the acetylcholine-induced responses by aminoguanidine was greater in the OVX. Endothelial dysfunction in OVX was...

Pharmacological Research, 2000

Pharmacological Research, 1996

This study was carried out to test the effect of chlorthalidone (Chlor) treatment on the vascular... more This study was carried out to test the effect of chlorthalidone (Chlor) treatment on the vascular responsiveness to acetylcholine (Ach) and adenosine disphosphate (ADP) under different extracellular calcium concentrations (Cae2+) in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Half of the DOCA-salt hypertensive rats were simultaneously given Chlor (8 mg day-1, p.o.), after the twentieth day of DOCA, until the fortieth day. Mean arterial pressure (MAP) was reduced (P < 0.01) in DOCA-salt-Chlor (121 +/- 3 mmHg) compared to DOCA-salt (175 +/- 3 mmHg) rats. Chlor reduced the high plasma sodium of DOCA-salt to levels of the normotensive groups. The basal perfusion pressure of the isolated mesenteric vascular bed (MVB) was not modified by Cae2+ changing or previous addition of ouabain. At normal and low Cae2+ the vasodilator effect of Ach in the MVB was reduced (P < 0.01) in DOCA-salt and less (P < 0.05) in the salt-control rats. Chlor shifted the curve of DOCA-salt backward to control group curves. In high Cae2+ the vasodilator responses to Ach were reduced (P < 0.01) both in normal-control and DOCA-salt+Chlor but not in salt-control but not in salt-control and DOCA-salt group. The vasodilator effect of ADP to different Cae2+ was similar in presence of normal and low Cae2+ in normal-control rats and it was reduced (P < 0.01) in presence of high Cae2+ in the DOCA-salt group. Chlor restored the responses to ADP to normal-control level. Under ouabain-treatment, the vasodilation duration was reduced (P < 0.05) in all groups. It was shorter (P < 0.01) in DOCA-salt than in the other ones. Chlor completely abolished this difference. The MAP reduction in DOCA-salt rats by chlor could also be associated with a restored normal responsiveness of endothelial cells to endogenous vasodilator, for example Ach and ADP, since sodium nitroprusside did not exhibit differences among the various treatments. This diuretic agent seems to elicit its effect through a reduction in plasma sodium content of DOCA-salt hypertensive rats.

Hypertension, 1994

The present study examined the time course of changes in baroreceptor reflex function by means of... more The present study examined the time course of changes in baroreceptor reflex function by means of sigmoidal curve-fitting analysis in conscious, unrestrained renovascular one-kidney, one clip (1K1C) rats at 1, 3, 7, 15, 30, and 60 days after renal artery clipping. The reflex heart rate responses were elicited by alternate intravenous bolus injections of phenylephrine (change, +5 to +50 mm Hg) and sodium nitroprusside (change, -5 to -50 mmHg). Atropine methylnitrate and atenolol were given to evaluate the responses mediated by the cardiac sympathetic or vagal component, respectively. The average baroreceptor reflex gain (sensitivity) decreased progressively (day 1, 3.35 +/- 0.3 beats per minute [bpm] per millimeter of mercury), reaching a maximal attenuation in the 30-day 1K1C group (1.83 +/- 0.5 bpm/mm Hg) compared with sham rats (approximately 4.60 bpm/mm Hg). The data showed a decreased vagal activity contributing to the attenuation of the baroreceptor gain only in the 30-day 1K1C...

Dermatologic Surgery, 2009

Clinical and Experimental Hypertension, 2010

The role of renal nerve in excretion was investigated during acute obstructive apnea (OA) episode... more The role of renal nerve in excretion was investigated during acute obstructive apnea (OA) episodes in SHR. The animals (SHR and control, C) were presented for renal denervation (D; CD; SHRD) or undenervation (U; CU; SHRU). Tracheal catheterization was performed to induce OA via its total occlusion. Urine samples were collected every 2 min after 20 s of OA. Obstructive apnea resulted in bradycardia, hypotension, and induced elevations in the urinary measurements in SHRU, but not in CU. Conversely, the denervation increased in CD, but not in the SHRD. Urinary excretion was dependent of renal nerve in SHR during OA.

Archives of Medical Research, 2008

Journal of International Medical Research, 1993

Chronic ethanol administration causes hypertension and alterations of vascular reactivity in rats... more Chronic ethanol administration causes hypertension and alterations of vascular reactivity in rats. In several models of hypertension, alterations of vascular reactivity are believed to be secondary to the sustained increase in blood pressure. The present study investigated the effects of serotonin (5-hydroxytryptamine [5-HT]), vasopressin and acetylcholine (ACh) in the isolated perfused mesenteric arteries from Wistar rats submitted to an 8-week course of chronic ethanol intake (8 g/kg day). No significant differences were observed in the dose – response curves with regard to: pressor effect of 0.04 — 10.0 nmole 5-HT; relaxant effect of 0.05 — 50.0 nmole ACh; or the pressor effects of two 1.5-nmole doses of vasopressin between control rats and ethanol-fed rats. These results suggest that modifications in arterial reactivity to endogenous vasoactive substances (observed in other studies involving more prolonged ethanol treatment in rats) may be, in part, secondary to the increase in ...