Nazli Saeedi - Academia.edu (original) (raw)

Papers by Nazli Saeedi

African Journal of Microbiology Research, 2011

Helicobacter pylori is a major gastroduodenal pathogen and its seropositivity is associated with ... more Helicobacter pylori is a major gastroduodenal pathogen and its seropositivity is associated with increased risk of development of human active chronic gastritis, peptic and duodenal ulcer and gastric cancer. The aim of this study was to determine the seroprevalence of H. pylori infection among subjects with gastrointestinal problems. A total of 339 serum samples collected from 114 male and 225 female were screened for detection of anti H. pylori IgG, IgA and IgM using commercial ELISA tests. The overall seropositivity rates were as follows: anti H. pylori IgG 73%; anti H. pylori IgM 43% and anti H. pylori IgA 25%. Seropositivity of anti H. pylori IgG increased markedly with age and highest infection rate (96%) was seen in individuals 40 to 50 years old. Anti H. pylori IgA was also correlated with increasing age. No association was detected between H. pylori seropositivity, gender and inflammatory laboratory parameters. This study revealed the high prevalence of H. pylori infection a...

Science and Research Branch, Islamic Azad University of Tehran, Tehran, Iran. Biotechnology Resea... more Science and Research Branch, Islamic Azad University of Tehran, Tehran, Iran. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Immunology Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Pharmaceutical Nanotechnology Research Center, Tabriz University of Medical sciences, Tabriz, Iran.

In the present study, we evaluated the impact of animal-origin food consumption on the recent pan... more In the present study, we evaluated the impact of animal-origin food consumption on the recent pandemic of Coronavirus-19 (COVID-19). Thus, the relationship among animal-origin food supply as independent factor and total cases of COVID-19 as a dependent variable was assessed. In this regard, the relevance between the consumption quantity of foods (n = 20) and TC of COVID-19 in worldwide countries (n = 215) was evaluated. For more details, we studied the number of total case (TC) as a dependent variable and food supply as an independent variable. Food supply (kg/capita/yr.) was estimated in each country based on the latest available data of FAO. The results showed an association between a group of animal-origin foods and TC. Regression, Bayes, and Lasso s findings demonstrated that eggs and freshwater fish have a high positive correlation with TC. We suppose an important role for animal-origin foods concerning COVID-19 as a cross-contamination pathway. In conclusion, a noticeable vehi...

Objectives: Brucellosis is a worldwide zoonotic disease with high morbidity in the absence of tre... more Objectives: Brucellosis is a worldwide zoonotic disease with high morbidity in the absence of treatment. The early diagnosis of brucellosis is efficient to prevent chronic infections. The aim of this study is evaluation of nested PCR efficiency in comparison with conventional methods for diagnosis of human brucellosis. A total of 120 patients with brucellosis symptoms were included in this study. Serological and microbiological tests and nested PCR were used for detection of Brucella bacteria. Results: Based on serological tests, 60.83% (73/120) of individuals were positive for brucellosis which only 8.33% of cases were confirmed by blood culture. Among them, 55% of cases were positive in serum agglutination test (SAT≥1:160) and Coombs (C-SAT≥1:160) tests. Furthermore, 7 negative SAT cases were positive in C-SAT as evidence for chronic brucellosis. Also, 68.18% and 56.06% of SAT positive samples were positive in blood nested PCR and serum nested PCR respectively. The sensitivity of ...

In recent months, nearly all countries tried to decrease human-to-human contact as the principal ... more In recent months, nearly all countries tried to decrease human-to-human contact as the principal mode of transmission of SARS-CoV-2. However, other modes of transmission also need to be clarified in more depth, especially, the foodborne transmission. We assessed the effect of animal origin foods consumption on the pandemic of COVID-19. For this purpose, we studied the relationship among 20 food supply as independent variables, and the parameter of Total Cases as dependent variable. Here we show a relationship between a group of animal origin foods and total cases. Regression, Bayes, and Lasso results showed that eggs and fresh water fish have positive coefficient. So, among the transmission ways of COVID_19, the role of foodborne transmission should be more significant than previously thought. The possibility of animal origin foodborne transmission should be taken into more consideration. The perspective is to expand the surveillance of SARS-Cov-2 during the food production chain. I...

Journal of Cellular Physiology

BioImpacts

Introduction: Cardiovascular diseases (CVDs) are recognized as the leading cause of mortality wor... more Introduction: Cardiovascular diseases (CVDs) are recognized as the leading cause of mortality worldwide. The increasing prevalence of such disease demand novel therapeutic and diagnostic approaches to overcome associated clinical/social issues. Recent advances in nanotechnology and biological sciences have provided intriguing insights to employ targeted nanosystems (NSs) to the desired location as multifunctional system for diagnosis, imaging and therapy of various diseases, including CVDs. Nanomedicines, as novel tool for improved drug delivery, imaging, and diagnosis, have shown great promise in combating the CVDs. Methods: In the current study, we intend to review the most recent studies on the nano-based strategies for improved management of the CVDs. Results: A cascade of biological events can result in the formation of atheromatous plaque and arterial stenosis, which need to be controlled. Recent studies have shown that nanomedicines can provide unique therapeutic benefits in the diagnosis and treatment of atherosclerosis. Conclusion: Despite some limitations, nanomedicines hold considerable potentials in the prevention, diagnosis, and treatment of various formidable diseases such as atherosclerosis. They offer various benefits, including maximal therapeutic effects with minimal side effects, amenable physicochemical properties such as surface modifications, and multifunctionality. Therefore, it is strongly envisioned that by the targeted drug delivery to the atherosclerotic lesions and plaque using nanomedicines, the onset and progression of CVDs can be efficiently managedin comparsion with the currently used classical treatment modalities.

Gastric Cancer

Helicobacter pylori affect around 50% of the population worldwide. More importantly, the gastric ... more Helicobacter pylori affect around 50% of the population worldwide. More importantly, the gastric infection induced by this bacterium is deemed to be associated with the progression of distal gastric carcinoma and gastric mucosal lymphoma in the human. H. pylori infection and its prevalent genotype significantly differ across various geographical regions. Based on numerous virulence factors, H. pylori can target different cellular proteins to modulate the variety of inflammatory responses and initiate numerous "hits" on the gastric mucosa. Such reactions lead to serious complications, including gastritis and peptic ulceration, gastric cancer and gastric mucosa-associated lymphoid structure lymphoma. Therefore, H. pylori have been considered as the type I carcinogen by the Global Firm for Research on Cancer. During the two past decades, different reports revealed that H. pylori possess oncogenic potentials in the gastric mucosa through a complicated interplay between the bacterial factors, various facets, and the environmental factors. Accordingly, numerous signaling pathways could be triggered in the development of gastrointestinal diseases (e.g., gastric cancer). Therefore, the main strategy for the treatment of gastric cancer is controlling the disease far before its onset using preventive/curative vaccination. Increasing the efficiency of vaccines may be achieved by new trials of vaccine modalities, which is used to optimize the cellular immunity. Taken all, H. pylori infection may impose severe complications, for resolving of which extensive researches are essential in terms of immune responses to H. pylori. We envision that H. pylori-mediated diseases can be controlled by advanced vaccines and immunotherapies.

Colloids and surfaces. B, Biointerfaces, 2018

Oxidative stress possesses a key role in the onset and development of cardiovascular diseases (CV... more Oxidative stress possesses a key role in the onset and development of cardiovascular diseases (CVDs), thus it can be an efficient target to tackle such ailment. Marrubiin, a bioactive diterpene, is a potent antioxidant against oxidative stress. Herein, we aimed to formulate marrubiin loaded solid lipid nanoparticles (SLNs) to improve its pharmacokinetics and bioavailability and also to investigate free drug and formulation's protective impact against intracellular reactive oxygen species (ROS) generation in HUVECs. Marrubiin-SLNs were formulated using hot homogenization/solidification method and then were subjected to physicochemical characterizations, i.e. size, zeta potential, morphology, polydispersity index (PDI), encapsulation efficiency (% EE), drug loading/content and physical stability assessments. MTT assay was performed to study the cytotoxicity of the intact and SLN incorporated marrubiin on HUVECs. Further, the antioxidant property of marrubiin and formulations was e...

Computational biology and chemistry, Jan 23, 2017

EG95 oncospheral antigen plays a crucial role in Echinococcus granulosus pathogenicity. Consideri... more EG95 oncospheral antigen plays a crucial role in Echinococcus granulosus pathogenicity. Considering the diversity of antigen among different EG95 isolates, it seems to be an ideal antigen for designing a universal multivalent minigene vaccine, so-called multi-epitope vaccine. This is the first in silico study to design a construct for the development of global EG95-based hydatid vaccine against E. granulosus in intermediate hosts. After antigen sequence selection, the three-dimensional structure of EG95 was modeled and multilaterally validated. The preliminary parameters for B-cell epitope prediction were implemented such as the possible transmembrane helix, signal peptide, post-translational modifications and allergenicity. The high ranked linear and conformational B-cell epitopes derived from several online web-servers (e.g., ElliPro, BepiPred v1.0, BcePred, ABCpred, SVMTrip, IEDB algorithms, SEPPA v2.0 and Discotope v2.0) were utilized for multiple sequence alignment and then for...

DNA and cell biology, 2016

In recent years, many researchers have focused on native plants to search for a new source of nat... more In recent years, many researchers have focused on native plants to search for a new source of natural components with medical approach, especially by means of anticancer potential. One of these natural components is Saqez, the resin of Pistacia atlantica sub-kurdica with the local name of Baneh. It has been reported as an anticancer and apoptosis inducer component; therefore, in this research, we aimed to evaluate the solvated resin's possible cyto/genotoxic effects. The cell viability was assessed using MTT assay. Flow cytometry analysis was performed to distinguish the role of apoptosis and necrosis in cell toxicity, which was further confirmed by Comet and DNA ladder assay, and 4,6-diamidino2-phenylindole (DAPI) staining. Pistacia atlantica's resin decreased the growth of the treated cells in a dose- and time-dependent manner, and single-strand DNA breaks have been observed through comet assay. Moreover, morphological changes of DAPI-stained cells showed fragmentation in ...

Http Dx Doi Org 10 4161 Hv 29032, May 8, 2014

Despite worldwide vaccination against devastating diseases for decades, millions of children in r... more Despite worldwide vaccination against devastating diseases for decades, millions of children in remote and impoverished regions of the globe die every year from vaccine-preventable infectious diseases. The reasons for incomplete coverage of vaccination programs are based in part on the relatively high costs of conventional vaccinations, including mass production, refrigeration, transportation, and training as well as funding personnel for their administration. Plant-based edible vaccines (PEVs) have been introduced as a revolutionary cost-effective vaccination modality. However, they suffer from major deficiencies that have restricted their application to bench-scale. This article discusses the deficiencies of PEVs and also provides concise overview on the health-promoting, biological and biotechnological features of spirulina (Arthrospira). In short, we envision that spirulina could be considered as a potential alternative biofactory system to the plants toward the production of edible vaccines in high-yield with low-costs that other hosts cannot yet offer.

African Journal of Biotechnology, Aug 9, 2013

Escherichia coli BL21 (DE3) and recombinant protein expression was analyzed by sodium dodecyl sul... more Escherichia coli BL21 (DE3) and recombinant protein expression was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Analyzing the expression of produced recombinant protein showed that the recombinant L2 can be highly expressed by this expression system. This recombinant protein can be used for the production of specific mAb, screening for specific ligands and competitive inhibition of the EGFR.

BioImpacts : BI, 2012

Essentially, people's diet and nutritional status has been changed substantially worldwide an... more Essentially, people's diet and nutritional status has been changed substantially worldwide and several lines of evidence suggest that these changes are to the detriment of their health. Additionally, it has been well documented that unhealthy diet especially the fast foods, untraditional foods or bad-eating-habits influence the human gut microbiome. The gut microbiota shapes immune responses during human life and affects his/her metabolomic profiles. Furthermore, many studies highlight the molecular pathways that mediate host and symbiont interactions that regulate proper immune function and prevention of cancer in the body. Intriguingly, if cancer forms in a human body due to the weakness of immune system in detriment of microbiome, the removal of cancer stem cells can be carried out through early Calories Restriction with Annual Fasting (AF) before tumor development or progress. Besides, fasting can balance the gut microbiome for enhancement of immune system against cancer for...

Human Vaccines & Immunotherapeutics, 2014

Enzyme Research, 2011

Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotid... more Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized in Leishmania infantum (L. infantum). The aim of this study was to clone and characterize the gene encoding class I nuclease in L. infantum. DNA extracted from L. infantum was used for amplification of P4 nuclease gene (Li-P4) by PCR. The product was cloned, sequenced, and expressed in E. coli for further characterization. Analysis of the sequence of Li-P4 revealed that the gene consists of an ORF of 951 bp. Sequence similarity analysis indicated that Li-P4 has a high homology to relevant enzymes of other kintoplastids with the highest homology (88%) to p1/s1 class I nuclease from L. donovani. Western blotting of antirecombinant Li-P4 with promastigote and amastigote stages of L. infantum showed that this nuclease is present in both stages of parasite with higher expression in amastigote stage. The highly conserved nature of this essential enzyme in Leishmania parasites suggests it as a promising drug target for leishmaniasis.

Future Microbiology, 2014

The Human Microbiome Project (HMP) revealed the significance of the gut microbiome in promoting h... more The Human Microbiome Project (HMP) revealed the significance of the gut microbiome in promoting health. Disruptions in microbiome composition are associated with the pathogenesis of numerous diseases. The indigenous microflora has co-evolved with humans for millions of years and humans have preserved the inherited microbiomes through consumption of fermented foods and interactions with environmental microbes. Through modernization, traditional foods were abandoned, native food starters were substituted with industrial products, vaccines and antibiotics were used, extreme hygiene measures were taken, the rate of cesarean section increased, and breast feeding changed into formula. These factors have reduced human exposure to microbial symbionts and led to shrinkage of the core microbiome. Reduction in microbiome biodiversity can compromise the human immune system and predispose individuals to several modern diseases. This article suggests launching microbiome biobanks for archiving native microbiomes, supervising antibiotic use, probiotic design and native starter production, as well as advertising a revisit to native lifestyles.

Journal of Cancer Research and Therapeutics, 2013

Sphingosine-1 phosphate (S1P) is a bioactive lipid that mediates diverse cellular responses. Sign... more Sphingosine-1 phosphate (S1P) is a bioactive lipid that mediates diverse cellular responses. Signaling of S1P is carried out by a family of G-protein coupled receptors (GPCRs), which show differential expression patterns depending on tissue and cell types. Activation of S1P receptors induces signaling pathway, which can subsequently lead to physiological process. Intercellular S1P concentration is regulated and determined by several enzymes including S1P lyase, S1P kinase and S1P phosphatase. Numerous studies showed the role of S1P in malignant behavior of cancer cells including breast, lung, colon, and leukemia cell lines. In the past decade, extensive research activities have focused on elucidating S1P signaling pathway, its receptors, enzymes involved in S1P metabolism, and its performance in cancer biology. In this review, we will explain the function of S1P in tumor progression that demonstrated in past research articles and we will express its importance as a target for designing futuristic anticancer drug.