Neha Sharma - Academia.edu (original) (raw)
Papers by Neha Sharma
Bioinorganic Research Laboratories, Department of Chemistry, Dr. H. S. Gour University,<br>... more Bioinorganic Research Laboratories, Department of Chemistry, Dr. H. S. Gour University,<br> Sagar-470 003, Madhya Pradesh, India<br> E-mail : apm19@rediffmail.com<br> Mauuscript received 15 June 2010, revised 31 January 2011, accepted 02 February 2011 The chemistry of metal complexes with tailor made Schiff base ligands and their applications, have arou~ed<br> considerable interest mainly because of preparative accessibility, diverse reactivity and structural variability. Complexes<br> of Nill and Cu11 with the Schiff bases viz. 3,4-dichloroaniline with 2-furfuraldehyde/4-dimethylaminobenzaldehyde and<br> 3-chloro-4-fluoroaniline with 4-dimethylaminobenzaldehyde have been synthesized. Physico-chemical characterization of<br> the complexes have been done with the help of micro-analytical data, UV-Vis, FT-IR, ESR spectroscopy, molar conductance,<br> thermal analysis, F AB-mass and magnetic susceptibility measurements. F AB-mass and therm...
2014 IEEE International Advance Computing Conference (IACC), 2014
ABSTRACT
Stem cell reports, Jan 11, 2014
Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TS... more Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic line...
Bioinorganic Research Laboratories, Department of Chemistry, Dr. H. S. Gour University,<br>... more Bioinorganic Research Laboratories, Department of Chemistry, Dr. H. S. Gour University,<br> Sagar-470 003, Madhya Pradesh, India<br> E-mail : apm19@rediffmail.com<br> Mauuscript received 15 June 2010, revised 31 January 2011, accepted 02 February 2011 The chemistry of metal complexes with tailor made Schiff base ligands and their applications, have arou~ed<br> considerable interest mainly because of preparative accessibility, diverse reactivity and structural variability. Complexes<br> of Nill and Cu11 with the Schiff bases viz. 3,4-dichloroaniline with 2-furfuraldehyde/4-dimethylaminobenzaldehyde and<br> 3-chloro-4-fluoroaniline with 4-dimethylaminobenzaldehyde have been synthesized. Physico-chemical characterization of<br> the complexes have been done with the help of micro-analytical data, UV-Vis, FT-IR, ESR spectroscopy, molar conductance,<br> thermal analysis, F AB-mass and magnetic susceptibility measurements. F AB-mass and therm...
2014 IEEE International Advance Computing Conference (IACC), 2014
ABSTRACT
Stem cell reports, Jan 11, 2014
Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TS... more Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic line...