Nelson Tsuno - Academia.edu (original) (raw)

Papers by Nelson Tsuno

Diagnostics, 2021

Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically signific... more Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mia, anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mia (377T) binds to 46DXHKRDTYA54, 48HKRDTYAAHT57 peptides, and anti-Mia (367T) binds to 43QTNDXHKRD51 peptides (X indicates T, M, or K). Anti-Mur is reactive with 49KRDTYPAHTA58 peptides. Anti-MUT is reactive with 47KHKRDTYA54...

Nippon Daicho Komonbyo Gakkai Zasshi

ISBT Science Series

ABSTRACT Platelet antigens/antibodies play an important role in immune mediated-thrombocytopenia.... more ABSTRACT Platelet antigens/antibodies play an important role in immune mediated-thrombocytopenia. Here, we review the clinical conditions associated with anti-platelet alloantibodies, giving special emphasis to the differences in human platelet antigen (HPA) frequency distribution between Caucasian and Asian populations. Also, we describe the presently available methods for the detection of anti-platelet alloantibodies, and the activities of the International Society of Blood Transfusion (ISBT) Platelet Immunology Working Parties, which play an active role in the exchange of information and materials among the labs involved in platelet immunobiology research worldwide, and have significantly contributed for the development of this field.

European Respiratory Journal, Sep 1, 2012

Hepato Gastroenterology, 2003

ABSTRACT

Hepato Gastroenterology, 2003

DPD (dihydropyrimidine dehydrogenase) activity shows a correlation with 5-fluorouracil chemosensi... more DPD (dihydropyrimidine dehydrogenase) activity shows a correlation with 5-fluorouracil chemosensitivity. To quantify DPD activity is important for selection of chemosensitive cases of not only sporadic colorectal cancer but also rectal cancer with preoperative radiotherapy. However, it is not cost-effective. We investigated the relation between the immunohistochemical expression pattern of DPD and its activity in rectal cancer treated with radiotherapy, and compared the immunohistochemical DPD expression pattern of preradiation biopsy specimens with that of resected tissues. DPD expression pattern of preradiation biopsy specimens were compared with that of resected tissues. Eighteen colorectal cancer tissue samples were obtained after surgery from October 2000 to January 2001. DPD activity was quantified by sandwich enzyme-linked immunosorbent assay. The streptoavidin-biotin peroxidase complex technique was used for the immunohistochemical expression pattern. DPD was stained in the cytoplasm of cancer cells. There was a significant correlation between the immunohistochemical expression pattern of DPD and its activity in tumor tissue treated with or without radiotherapy. The immunohistochemical expression pattern of preradiation biopsy specimens was almost the same as that of resected tissues. The immunohistochemical expression pattern of DPD correlated with its activity in tumor tissue treated with preoperative radiotherapy. Immunohistochemical evaluation is considered to be an effective method of predicting the sensitivity to 5-fluorouracil of rectal cancer treated with preoperative radiotherapy.

Texas Heart Institute Journal from the Texas Heart Institute of St Luke S Episcopal Hospital Texas Children S Hospital, 2011

The KW39 stent is a balloon-expandable, stainless-steel, slotted-tube stent, newly designed to ad... more The KW39 stent is a balloon-expandable, stainless-steel, slotted-tube stent, newly designed to adjust to the shape of the coronary arteries. We evaluated the clinical efficacy and safety of KW39 stent-based percutaneous coronary interventions in human native coronary arteries. A total of 105 patients (110 lesions), with a diagnosis of stable angina, acute coronary syndrome, or asymptomatic myocardial ischemia, were included in this prospective study. The primary endpoint was the target-lesion revascularization rate at the conclusion of a 6-month follow-up period. The secondary endpoints were the rates of technical and procedural success and the rate of major adverse cardiac events (defined as cardiac death, myocardial infarction, and target-lesion revascularization) in the course of the 6 months after stent placement. The 6-month target-lesion revascularization rate was 8.6%. The KW39 stent was highly satisfactory in regard to all secondary endpoint comparisons. Binary (>50%) in-stent restenosis was observed in 22 of 110 lesions (20%). The mean diameter stenosis at 6 months after percutaneous coronary intervention was 35.1% ± 14.4%, and the mean late lumen loss was 1.06 ± 0.48 mm. Stepwise multivariate analysis showed probable causal associations between adverse local environments for stent implantation and the subsequent need for target-lesion revascularization. We conclude that KW39 stent implantation was technically feasible and clinically safe in the patient population that we studied. The results of the safety endpoints, including cardiac death and acute myocardial infarction, were acceptable.

IL-17A is a T cell-derived proinflammatory cytokine that contributes to the pathogenesis of rheum... more IL-17A is a T cell-derived proinflammatory cytokine that contributes to the pathogenesis of rheumatoid arthritis. Recently, six related molecules have been identified to form the IL-17 family, as follows: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F. Whereas IL-17A and IL-17F up-regulate IL-6 in synovial fibroblasts, IL-17B and IL-17C are reported to stimulate the release of TNF- and IL-1 from the

Exposure of cells to extremely low frequency (ELF) electromagnetic fields which reportedly induce... more Exposure of cells to extremely low frequency (ELF) electromagnetic fields which reportedly induced the transcription of heat shock protein 70 (HSP70) 1,2, was recently reputed by other studies. HSP is a group of proteins produced by cells in response to stress. We hypothesized that repetitive pulse magnetic stimulation, a much stronger electromagnetic stimuli than those used in the past studies, could induce the stress response of cells.

ISBT Science Series, 2014

ABSTRACT Alloantibodies to human neutrophil antigens (HNA) are involved in clinical conditions su... more ABSTRACT Alloantibodies to human neutrophil antigens (HNA) are involved in clinical conditions such as neonatal alloimmune neutropenia (NAN), autoimmune neutropenia (AIN) and transfusion-related acute lung injury (TRALI). For the diagnosis of these conditions, the detection of the causative antibody is essential. Presently, the use of a combination of granulocyte agglutination assay (GAT) and granulocyte immunofluorescence test (GIFT) for the screening of granulocyte antibodies, followed by the determination of antibody specificity by the monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) is recommended by the ISBT Working Party on Granulocyte Immunobiology. In Japan, the mixed-passive hemagglutination (MPHA) assay, a method originally developed for the detection of anti-platelet antibodies, was adapted for the testing of anti-granulocyte antibodies. Although various technologies are available, presently, no one of the methods alone is able to detect all clinically relevant antibodies, thus their combination is important, and the development of new methodologies is desired. In this review, we describe the presently available classical methods for anti-granulocyte antibody detection, including GIFT, GAT, MAIGA and MPHA, and also the new technologies, especially focusing on the advantages and problems of the different methods.

Abcd Arq Bras Cir Dig, Sep 1, 1993

Critical Care Medicine, 1998

Recent Advances in Management of Digestive Cancers, 1993

In an attempt to clarify the role of lateral node dissection in preventing the local recurrence o... more In an attempt to clarify the role of lateral node dissection in preventing the local recurrence of rectal cancer, 203 patients undergoing surgery for advanced lower rectal cancer in our surgical department from 1963 to 1991 were studied retrospectively. The incidence of lateral lymphnode metastases was higher in patients with tumors invading beyond the subserosa and a higher recurrence rate was observed in patients with lymphnode metastasis diagnosed at or before operation. Lymphnode metastases were found in 18.2% of the patients undergoing lateral node dissection, and surgery was succeeded in 66.7% of these patients, although 50% of the cases had tumors invading beyond the subserosal layer. The recurrence rate was 33.3%. We concluded that the lateral node dissection plays an important role in preventing local recurrence of rectal cancer, and should be indicated for all patients with either lymphnode metastases or tumors invading beyond the subserosal layer.

Diseases of the Colon & Rectum

ABSTRACT

Journal of Gastroenterology

World journal of surgical oncology, Jan 7, 2015

Although neoadjuvant chemoradiotherapy (CRT) has become a standard procedure to downstage locally... more Although neoadjuvant chemoradiotherapy (CRT) has become a standard procedure to downstage locally advanced rectal cancer prior to surgery, markers to predict the response to CRT have not been fully identified. The aim of this study was to identify predictive factors of response to CRT, especially focusing on peripheral blood leukocyte subsets. A total of 45 consecutive patients diagnosed with primary rectal cancer were prospectively enrolled and received CRT followed by curative resection. The numbers of each lymphocyte subset in peripheral blood pre- and post-CRT were analyzed using flow cytometry. According to the pathological response to CRT, patients were classified into high (Hi-R) and low (Lo-R) response groups. Hi-R cases had significantly higher numbers of pre-CRT lymphocytes (p = 0.018), T lymphocytes (p = 0.009) and helper T lymphocytes (Th lymphocytes, p = 0.015) compared to the Lo-R cases. With the receiver-operating characteristic curve for numbers of pre-CRT T lymphocy...

International surgery, 2015

Allogeneic blood transfusion (ABT) has been reported as a major risk factor for surgical site inf... more Allogeneic blood transfusion (ABT) has been reported as a major risk factor for surgical site infection (SSI) in patients undergoing colorectal surgery. However, the association of ABT with SSI in patients undergoing abdominoperineal resection (APR) and total pelvic exenteration (TPE) still remains to be evaluated. Here, we aim to elucidate this association. The medical records of all patients undergoing APR and TPE at our institution in the period between January 2000 and December 2012 were reviewed. Patients without SSI (no SSI group) were compared with patients who developed SSI (SSI group), in terms of clinicopathologic features, including ABT. In addition, data for 262 patients who underwent transabdominal rectal resection at our institution in the same period were also enrolled, and their data on differential leukocyte counts were evaluated. Multivariate analysis showed that intraoperative transfusion was an independent predictive factor for SSI after APR and TPE (P = 0.004). ...

Nippon Daicho Komonbyo Gakkai Zasshi

Diagnostics, 2021

Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically signific... more Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mia, anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mia (377T) binds to 46DXHKRDTYA54, 48HKRDTYAAHT57 peptides, and anti-Mia (367T) binds to 43QTNDXHKRD51 peptides (X indicates T, M, or K). Anti-Mur is reactive with 49KRDTYPAHTA58 peptides. Anti-MUT is reactive with 47KHKRDTYA54...

Nippon Daicho Komonbyo Gakkai Zasshi

ISBT Science Series

ABSTRACT Platelet antigens/antibodies play an important role in immune mediated-thrombocytopenia.... more ABSTRACT Platelet antigens/antibodies play an important role in immune mediated-thrombocytopenia. Here, we review the clinical conditions associated with anti-platelet alloantibodies, giving special emphasis to the differences in human platelet antigen (HPA) frequency distribution between Caucasian and Asian populations. Also, we describe the presently available methods for the detection of anti-platelet alloantibodies, and the activities of the International Society of Blood Transfusion (ISBT) Platelet Immunology Working Parties, which play an active role in the exchange of information and materials among the labs involved in platelet immunobiology research worldwide, and have significantly contributed for the development of this field.

European Respiratory Journal, Sep 1, 2012

Hepato Gastroenterology, 2003

ABSTRACT

Hepato Gastroenterology, 2003

DPD (dihydropyrimidine dehydrogenase) activity shows a correlation with 5-fluorouracil chemosensi... more DPD (dihydropyrimidine dehydrogenase) activity shows a correlation with 5-fluorouracil chemosensitivity. To quantify DPD activity is important for selection of chemosensitive cases of not only sporadic colorectal cancer but also rectal cancer with preoperative radiotherapy. However, it is not cost-effective. We investigated the relation between the immunohistochemical expression pattern of DPD and its activity in rectal cancer treated with radiotherapy, and compared the immunohistochemical DPD expression pattern of preradiation biopsy specimens with that of resected tissues. DPD expression pattern of preradiation biopsy specimens were compared with that of resected tissues. Eighteen colorectal cancer tissue samples were obtained after surgery from October 2000 to January 2001. DPD activity was quantified by sandwich enzyme-linked immunosorbent assay. The streptoavidin-biotin peroxidase complex technique was used for the immunohistochemical expression pattern. DPD was stained in the cytoplasm of cancer cells. There was a significant correlation between the immunohistochemical expression pattern of DPD and its activity in tumor tissue treated with or without radiotherapy. The immunohistochemical expression pattern of preradiation biopsy specimens was almost the same as that of resected tissues. The immunohistochemical expression pattern of DPD correlated with its activity in tumor tissue treated with preoperative radiotherapy. Immunohistochemical evaluation is considered to be an effective method of predicting the sensitivity to 5-fluorouracil of rectal cancer treated with preoperative radiotherapy.

Texas Heart Institute Journal from the Texas Heart Institute of St Luke S Episcopal Hospital Texas Children S Hospital, 2011

The KW39 stent is a balloon-expandable, stainless-steel, slotted-tube stent, newly designed to ad... more The KW39 stent is a balloon-expandable, stainless-steel, slotted-tube stent, newly designed to adjust to the shape of the coronary arteries. We evaluated the clinical efficacy and safety of KW39 stent-based percutaneous coronary interventions in human native coronary arteries. A total of 105 patients (110 lesions), with a diagnosis of stable angina, acute coronary syndrome, or asymptomatic myocardial ischemia, were included in this prospective study. The primary endpoint was the target-lesion revascularization rate at the conclusion of a 6-month follow-up period. The secondary endpoints were the rates of technical and procedural success and the rate of major adverse cardiac events (defined as cardiac death, myocardial infarction, and target-lesion revascularization) in the course of the 6 months after stent placement. The 6-month target-lesion revascularization rate was 8.6%. The KW39 stent was highly satisfactory in regard to all secondary endpoint comparisons. Binary (>50%) in-stent restenosis was observed in 22 of 110 lesions (20%). The mean diameter stenosis at 6 months after percutaneous coronary intervention was 35.1% ± 14.4%, and the mean late lumen loss was 1.06 ± 0.48 mm. Stepwise multivariate analysis showed probable causal associations between adverse local environments for stent implantation and the subsequent need for target-lesion revascularization. We conclude that KW39 stent implantation was technically feasible and clinically safe in the patient population that we studied. The results of the safety endpoints, including cardiac death and acute myocardial infarction, were acceptable.

IL-17A is a T cell-derived proinflammatory cytokine that contributes to the pathogenesis of rheum... more IL-17A is a T cell-derived proinflammatory cytokine that contributes to the pathogenesis of rheumatoid arthritis. Recently, six related molecules have been identified to form the IL-17 family, as follows: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F. Whereas IL-17A and IL-17F up-regulate IL-6 in synovial fibroblasts, IL-17B and IL-17C are reported to stimulate the release of TNF- and IL-1 from the

Exposure of cells to extremely low frequency (ELF) electromagnetic fields which reportedly induce... more Exposure of cells to extremely low frequency (ELF) electromagnetic fields which reportedly induced the transcription of heat shock protein 70 (HSP70) 1,2, was recently reputed by other studies. HSP is a group of proteins produced by cells in response to stress. We hypothesized that repetitive pulse magnetic stimulation, a much stronger electromagnetic stimuli than those used in the past studies, could induce the stress response of cells.

ISBT Science Series, 2014

ABSTRACT Alloantibodies to human neutrophil antigens (HNA) are involved in clinical conditions su... more ABSTRACT Alloantibodies to human neutrophil antigens (HNA) are involved in clinical conditions such as neonatal alloimmune neutropenia (NAN), autoimmune neutropenia (AIN) and transfusion-related acute lung injury (TRALI). For the diagnosis of these conditions, the detection of the causative antibody is essential. Presently, the use of a combination of granulocyte agglutination assay (GAT) and granulocyte immunofluorescence test (GIFT) for the screening of granulocyte antibodies, followed by the determination of antibody specificity by the monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) is recommended by the ISBT Working Party on Granulocyte Immunobiology. In Japan, the mixed-passive hemagglutination (MPHA) assay, a method originally developed for the detection of anti-platelet antibodies, was adapted for the testing of anti-granulocyte antibodies. Although various technologies are available, presently, no one of the methods alone is able to detect all clinically relevant antibodies, thus their combination is important, and the development of new methodologies is desired. In this review, we describe the presently available classical methods for anti-granulocyte antibody detection, including GIFT, GAT, MAIGA and MPHA, and also the new technologies, especially focusing on the advantages and problems of the different methods.

Abcd Arq Bras Cir Dig, Sep 1, 1993

Critical Care Medicine, 1998

Recent Advances in Management of Digestive Cancers, 1993

In an attempt to clarify the role of lateral node dissection in preventing the local recurrence o... more In an attempt to clarify the role of lateral node dissection in preventing the local recurrence of rectal cancer, 203 patients undergoing surgery for advanced lower rectal cancer in our surgical department from 1963 to 1991 were studied retrospectively. The incidence of lateral lymphnode metastases was higher in patients with tumors invading beyond the subserosa and a higher recurrence rate was observed in patients with lymphnode metastasis diagnosed at or before operation. Lymphnode metastases were found in 18.2% of the patients undergoing lateral node dissection, and surgery was succeeded in 66.7% of these patients, although 50% of the cases had tumors invading beyond the subserosal layer. The recurrence rate was 33.3%. We concluded that the lateral node dissection plays an important role in preventing local recurrence of rectal cancer, and should be indicated for all patients with either lymphnode metastases or tumors invading beyond the subserosal layer.

Diseases of the Colon & Rectum

ABSTRACT

Journal of Gastroenterology

World journal of surgical oncology, Jan 7, 2015

Although neoadjuvant chemoradiotherapy (CRT) has become a standard procedure to downstage locally... more Although neoadjuvant chemoradiotherapy (CRT) has become a standard procedure to downstage locally advanced rectal cancer prior to surgery, markers to predict the response to CRT have not been fully identified. The aim of this study was to identify predictive factors of response to CRT, especially focusing on peripheral blood leukocyte subsets. A total of 45 consecutive patients diagnosed with primary rectal cancer were prospectively enrolled and received CRT followed by curative resection. The numbers of each lymphocyte subset in peripheral blood pre- and post-CRT were analyzed using flow cytometry. According to the pathological response to CRT, patients were classified into high (Hi-R) and low (Lo-R) response groups. Hi-R cases had significantly higher numbers of pre-CRT lymphocytes (p = 0.018), T lymphocytes (p = 0.009) and helper T lymphocytes (Th lymphocytes, p = 0.015) compared to the Lo-R cases. With the receiver-operating characteristic curve for numbers of pre-CRT T lymphocy...

International surgery, 2015

Allogeneic blood transfusion (ABT) has been reported as a major risk factor for surgical site inf... more Allogeneic blood transfusion (ABT) has been reported as a major risk factor for surgical site infection (SSI) in patients undergoing colorectal surgery. However, the association of ABT with SSI in patients undergoing abdominoperineal resection (APR) and total pelvic exenteration (TPE) still remains to be evaluated. Here, we aim to elucidate this association. The medical records of all patients undergoing APR and TPE at our institution in the period between January 2000 and December 2012 were reviewed. Patients without SSI (no SSI group) were compared with patients who developed SSI (SSI group), in terms of clinicopathologic features, including ABT. In addition, data for 262 patients who underwent transabdominal rectal resection at our institution in the same period were also enrolled, and their data on differential leukocyte counts were evaluated. Multivariate analysis showed that intraoperative transfusion was an independent predictive factor for SSI after APR and TPE (P = 0.004). ...

Nippon Daicho Komonbyo Gakkai Zasshi