Nemanja Jezdimirovic - Academia.edu (original) (raw)

Papers by Nemanja Jezdimirovic

Zenodo (CERN European Organization for Nuclear Research), Jun 15, 2013

Универзитет у Београду, Jul 11, 2019

Veliku zahvalnost za pomoć pri izradi ove disertacije dugujem: • svom mentoru profesoru dr Milija... more Veliku zahvalnost za pomoć pri izradi ove disertacije dugujem: • svom mentoru profesoru dr Milijanu Jovanoviću, na poverenju, znanju i iskustvu koje mi je preneo, strpljenju da me sasluša, na svim savetima i trudu da ova doktorska disertacija ugleda svetlo dana. • profesoru dr Dejanu Krnjaiću, na ukazanom poverenju i nesebičnoj podršci pri izradi ove disertacije. • dr Branislavu Kureljušiću, bih se zahvalio na bezuslovnoj pomoći, na strpljenju, nesebičnim savetima i sugestijama u izradi ove doktorske disertacije. • kolektivu Naučnog instituta za veterinarstvo Srbije, direktorki dr Dobrili Jakić Dimić, dr Oliveru Radanoviću, svim zaposlenima, tehničkom saradniku Milošu Raduloviću, na ukazanom poverenju i interesovanju sa kojim su pratili moj rad. • Mojoj porodici, supruzi Jeleni, mom sinu Božidaru, mojoj ćerki Sofiji, na svim osmesima, pažnji, poljupcima i bezuslovnoj ljubavi koju su mi pružili i koji mi nisu dali da odustanem. • Mojim roditeljima, mojoj majci, ocu, sestri Ani, što su verovali u mene, što su uvek bili uz mene, što su mi pružali ljubav i podršku svih ovih godina. Majci, ocu i mojoj sestri Ani koji su me učinili dobrim čovekom, dobrim sinom i dobrim bratom. Na kraju bih želelo da izrazim posebno veliku zahvanost mom dragom učitelju, dr Vojinu Ivetiću na nesebičnoj velikoj pomoći i ličnom i profesionalnom zalaganju u izradi doktorske disertacije, na svim prijateljskim savetima, razumevanju, stručnim sugestijama, na ogromnoj podršci, na očinskim savetima i na kraju što mi nije dao da se predam nego da nastavim... Mojim anđelima, zbog kojih postojim, Boži i Sofiji... Dvadesetčetiri sata od aplikacije fitohemaglutinina prosečna vrednost debljine kože (DK) kod inficiranih i deksametazonom tretiranih ćurića (O-2) bila je statistički veoma značajno manja (p<0,001) u odnosu na prosečnu vrednost DK kod inficiranih ćurića (O-1). Rezultati hematoloških ispitivanja pokazali su da je infekcija ćurića gljivicom A. fumigatus prouzrokovala slabu anemiju, leukocitozu, limfocitozu i heterofiliju, posle druge i treće nedelje. PCR metodom A. fumigatus iz zbirnih uzoraka pluća i vazdušnih kesa, srca, jetre i mozga ćurića posle 1, 3, 7, 14. i 21. dana od veštačkog inficiranja ustanovljen je veći broj pozitivnih uzoraka na prisustvo DNK A. fumigatus kod ćurića ogledne grupe 2.

Acta Veterinaria-beograd, Sep 23, 2017

Infectious arthritides in goats is mainly caused by mycoplasmas and Caprine Arthritis-Encephaliti... more Infectious arthritides in goats is mainly caused by mycoplasmas and Caprine Arthritis-Encephalitis virus (CAEV). Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) is a member of Lancefi eld group C beta-hemolytic streptococcus that commonly colonizes the mucous membranes of healthy equids which is also capable to induce different pathological conditions in many animal species. In this paper we described a case of polyarthritis in four Alpine goat-kids caused by S. zooepidemicus. Goat-kids originated from a farm where sheep, llamas, shepherd dogs, cows and horses were kept with goats. During the external examination of carcasses, crusts and discrete hyperemia were seen on the earlobes beneath the ear tag, as well as swollen joints. Necropsy fi ndings included severe fi brinous polyarthritis in all four examined kids. Shoulder, knee and hip joints were affected. From all affected joints S. zooepidemicus was isolated. Neither Mycoplasma spp. nor CAEV genome were detected from the affected joints. Most likely the goatkids were infected with S. zooepidemicus through the lesions on earlobes which were made after the tagging. As a possible source of bacteria, we recognized the horses and dogs. Due to the lack of any signs of illness in other animal species on the farm, as well as negative pathomorphological fi ndings in other organs, we assumed that the isolated strain is highly adapted to goat-kids. Our report is the fi rst described case of polyarthritis in goat-kids caused by S. zooepidemicus.

Journal of Veterinary Diagnostic Investigation, Jul 8, 2021

Salmonella enterica subspecies enterica serovar Choleraesuis is rarely detected in Europe, but th... more Salmonella enterica subspecies enterica serovar Choleraesuis is rarely detected in Europe, but the clinical disease has been reported in wild boars. We describe here the clinical findings, pathologic changes, and microbiologic features of swine salmonellosis caused by S. enterica serovar Choleraesuis in weaned piglets in Serbia. In April 2019, on a large farrow-to-finish pig farm, increased mortality was reported in weaned piglets, marked by lethargy, anorexia, pyrexia, and respiratory distress. Gross pathology revealed dermal cyanosis, mesenteric lymphadenopathy, splenomegaly, hepatomegaly, interstitial pneumonia, and colitis. By direct culturing of lung, liver, spleen, and lymph nodes, S. enterica ser. Choleraesuis variant Kunzendorf was isolated after years of absence of the disease in pig farms in Europe. The source of this salmonellosis outbreak caused by S. enterica ser. Choleraesuis remains unknown.

Polish Journal of Veterinary Sciences, Dec 1, 2016

Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postparta... more Bovine herpesvirus type 4 (BHV-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. In this study a retrospective PCR examination of 100 extracted DNA samples from aborting cows was performed in order to determine: prevalence of BHV-4 in abortive cattle, whether coinfections BHV-4 with other abortifacient pathogens are present in the same sample and to determine the month of gestation when BHV-4 associated abortions were detected. Out of 100 examined samples, the BHV-4 genome was detected in 21 samples (21%). In two samples we detected coinfection of BHV-4 with bovine viral diarrhea virus (BVDV) and in one with Neospora caninum. Most of the BHV-4-associated abortions were detected during the seventh month of gestation. It was concluded that an active BHV-4 infection was present among cows that aborted on the farms examined. The high prevalence of the BHV-4 genome in abortion material suggests that this virus may have cause the abortions. Further studies and examinations are needed to establish causative connection between presence of BHV-4 and abortion.

Contrary to pig farming in developed western countries, in a large part of the world, pigs are st... more Contrary to pig farming in developed western countries, in a large part of the world, pigs are still traditionally kept, in small backyard farms, usually for family needs. Their main characteristics are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from 69 backyard holdings were randomly selected and tested for PRRS, Aujeszky`s disease, PPV infection and Swine influenza by ELISA. The herd-level seroprevalence of PRRS, Aujeszky`s disease and PPV infection was 2.9%, 27.5% and 37.7%, respectively. Swine influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed PPV and AD in backyard farms in Serbia, almost...

Journal of the Hellenic Veterinary Medical Society

A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Instit... more A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Institute of Veterinary Medicine of Serbia for determining the potential cause of death. Necropsy revealed massive hemorrhages in subcutaneous and intermuscular tissue and on papillary muscle. Petechiae and ecchymoses were found on the omentum, mesentery and adipose tissue of heart, kidney and costal pleura. Haemorrhagic-necrotic enteritis in duodenum and jejunum was characterized by catarrhal hemorrhagic inflammation with the presence of mucous and bloody content, whereas gas bubbles in the submucosa have also been confirmed. Bacterial cultures from sampled organs were identified as Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli. Based on the established pathological and histological changes and the results of the bacteriological, biochemical, and molecular examination, the state of septic shock and toxemia with disseminated massive bleeding was the immediate ...

Zenodo (CERN European Organization for Nuclear Research), Jun 15, 2012

Veterinaria Italiana, Dec 31, 2021

Spirocerosis is a parasitic disease caused by the nematode Spirocerca lupi, which has often fatal... more Spirocerosis is a parasitic disease caused by the nematode Spirocerca lupi, which has often fatal outcome in dogs. The disease is rarely diagnosed due to nonspecific clinical signs and the lack of awareness of the presence of the agent among practitioners. The first case of spirocercosis in dogs in Serbia was recorded in Belgrade in 2014 and in the later period until 2019, eighteen more cases in the wider area of Belgrade were discovered.

Acta Veterinaria Hungarica, 2021

At a commercial farrow-to-feeder pig system with 2,100 sows in Serbia, lesions resembling porcine... more At a commercial farrow-to-feeder pig system with 2,100 sows in Serbia, lesions resembling porcine ear necrosis syndrome were observed in 80% of the weaned pigs at 45–50 days of age. Pathomorphological examinations were carried out on 10 pigs that had been found dead. The gross lesions ranged from mild, superficial dermatitis to severe, deep inflammation with exudation, ulceration and necrosis. Histopathological examination revealed erosive and ulcerative dermatitis of the pinna with neutrophilic and lymphocytic infiltration and bacterial colonies in the crusts. Staphylococcus aureus (MRSA strain), Staphylococcus hyicus and Streptococcus group C were cultivated from eight, S. hyicus from two ear tissue scraping samples. All 10 samples were positive for treponemes and phylogenetic analysis of two polymerase chain reaction products confirmed the relationship to Treponema (T.) medium/vincentii and Treponema pedis. Treponemes were also detected in seven oral swabs that were analysed to o...

Archives of Veterinary Medicine, 2018

Vaccines are a very effective tool for the prevention and eradication of infective diseases in bo... more Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse rea...

The Journal of Infection in Developing Countries, 2020

Introduction: Hepatitis E is considered an emerging human viral disease with many evidences of zo... more Introduction: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. Methodology: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. Results: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaug...

Acta Veterinaria, 2019

The aim of this study was to determine the pathological, mycological and molecular findings in tu... more The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056×107 spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the...

Acta veterinaria, 2013

The aim of this paper was to investigate the morphology, localization and typization of abomasal ... more The aim of this paper was to investigate the morphology, localization and typization of abomasal ulcers in high-yielding dairy cows, as a contribution to the existing knowledge on the subject. A total of ten high-yielding dairy cows which died during the period of early puerperium and two Holstein heifers which died in late pregnancy after transport to a new location were investigated in this study. Samples of altered abomasal tissue were taken at necropsy for further histopathology and mycology investigations. The disclosed ulcerations of the abomasums in twelve investigated animals differed among them not only as far as localization and morphology are concerned, but also in the degree of intramural penetration. Such differences distinguished them into four types. Ulcerations of type one and type two, recorded in two heifers and six cows, as well as in one cow with lymphoma of the abomasum. Perforating ulcer of type three, with circumscripted peritonitis was diagnosed in one cow in the corpus of the abomasum close to the curvatura major. An interesting finding was the simultaneous occurrence of type four perforating ulcer and ulcer-type one, which were located next to each other. Histopathological examination of the structure of the ulcerated abomasum dyed with the hematoxylin-eosin method showed that coagulation necrosis in the area of the ulceration revealed a moderate inflammatory infiltrate in the lamina propria. In the cow affected with lymphoma, a copious tumorous lymphoid infiltrate was concurrent. Mycotic ulcerative abomasitis was described in a cow seven days after calving. In these lesions Aspergillus fumigatus and Mucor spp. were isolated. Histopathological analysis of tissue samples dyed with the Grocott method, described septed and nonsepted hyphae in the blood vessels and surrounding tissue of the tunica mucosa and submucosa.

Animals

Contrary to pig farming in developed Western countries, in a large part of the world, pigs are st... more Contrary to pig farming in developed Western countries, in a large part of the world, pigs are still traditionally kept in small backyard farms, usually for family needs. Their main characteristics are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from 69 backyard holdings were randomly selected and tested for antibodies to Porcine Reproductive and Respiratory Syndrome virus (PRRSV), Aujeszky’s disease virus (ADV), Porcine Parvovirus (PPV) and Swine influenza Virus (SIV) by enzyme-linked immunosorbent assay (ELISA). The herd-level seroprevalence of PRRS, Aujeszky’s disease and PPV was 2.9%, 27.5% and 37.7%, respectively. Swine influenza seroconv...

West Nile fever is a vector borne viral disease that can affect humans, horses, birds and sometim... more West Nile fever is a vector borne viral disease that can affect humans, horses, birds and sometimes other species of animals. Every year West Nile fever is detected in the human population in Serbia. The disease often occurs in a subclinical form, but most clinically evident cases occur in horses. Therefore, horses are recommended as a sentinel species for monitoring the general incidence of West Nile fever in a specific territory. Our goal was to determine the prevalence of antibodies against West Nile fever virus in horses in the Belgrade epizootiological area. We examined serum samples from 77 horses to determine the seroprevalence of West Nile fever virus in horses throughout the city of Belgrade. Sera were tested by commercial ELISA tests for detection of specific IgG-class antibodies to West Nile fever virus and for the detection of specific IgM-class antibodies to confirm the presence of old and acute (recent) infections in horses. The results confirmed that West Nile fever v...

Slovenian Veterinary Research, 2014